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Conserved domains on  [gi|697053314|ref|WP_033145920|]
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MULTISPECIES: DgsA anti-repressor MtfA [Enterobacter]

Protein Classification

DgsA anti-repressor MtfA( domain architecture ID 10015108)

DgsA anti-repressor MtfA is a zinc metallopeptidase that functions as a regulator of the phosphoenolpyruvate (PEP)-dependent:glucose phosphotransferase system (ptsG) by binding and inactivating DgsA (aka Mlc), a repressor of ptsG; belongs to the M90 peptidase family

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PRK15410 PRK15410
DgsA anti-repressor MtfA; Provisional
1-260 0e+00

DgsA anti-repressor MtfA; Provisional


:

Pssm-ID: 185308  Cd Length: 260  Bit Score: 538.17  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   1 MIKWPWKTNEAGRDMALPWDDALTIPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLELG 80
Cdd:PRK15410   1 MIKWPWKAQEPAHQTALPWQEALSIPLLTPLTEQEQQKLVALAERFLQQKRLVPLQGLELDSLMSARIALLFCLPVLELG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  81 IEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSFDASGFNLVIHEVAHKLDTRNGD 160
Cdd:PRK15410  81 IEWLDGFHEVLIYPAPFVVDDEWEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDMRNGD 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 161 RASGVPLIPLREVAGWEHDLHAAMDNIQDEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQ 240
Cdd:PRK15410 161 RASGVPFIPLREVAGWEHDLHAAMNNIQEEIDLVGENAASIDAYAASDPAECFAVLSEYFFSAPELFAPRFPALYQRFCQ 240
                        250       260
                 ....*....|....*....|
gi 697053314 241 FYQQDPLQRLRQNEESGGHS 260
Cdd:PRK15410 241 FYRQDPLQRLHRWNDTDSFS 260
 
Name Accession Description Interval E-value
PRK15410 PRK15410
DgsA anti-repressor MtfA; Provisional
1-260 0e+00

DgsA anti-repressor MtfA; Provisional


Pssm-ID: 185308  Cd Length: 260  Bit Score: 538.17  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   1 MIKWPWKTNEAGRDMALPWDDALTIPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLELG 80
Cdd:PRK15410   1 MIKWPWKAQEPAHQTALPWQEALSIPLLTPLTEQEQQKLVALAERFLQQKRLVPLQGLELDSLMSARIALLFCLPVLELG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  81 IEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSFDASGFNLVIHEVAHKLDTRNGD 160
Cdd:PRK15410  81 IEWLDGFHEVLIYPAPFVVDDEWEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDMRNGD 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 161 RASGVPLIPLREVAGWEHDLHAAMDNIQDEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQ 240
Cdd:PRK15410 161 RASGVPFIPLREVAGWEHDLHAAMNNIQEEIDLVGENAASIDAYAASDPAECFAVLSEYFFSAPELFAPRFPALYQRFCQ 240
                        250       260
                 ....*....|....*....|
gi 697053314 241 FYQQDPLQRLRQNEESGGHS 260
Cdd:PRK15410 241 FYRQDPLQRLHRWNDTDSFS 260
MtfA COG3228
Mlc titration factor MtfA, regulates ptsG expression [Signal transduction mechanisms];
1-248 3.44e-108

Mlc titration factor MtfA, regulates ptsG expression [Signal transduction mechanisms];


Pssm-ID: 442461  Cd Length: 253  Bit Score: 312.87  E-value: 3.44e-108
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   1 MIKWP--WKTNEAGRDMALP--WDDALT-IPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLP 75
Cdd:COG3228    1 MLRWLrrWRRRRRLARAPFPalWRAILArVPFLAGLSAEERARLRELAQLFLAEKRFEGVGGLEVTDEMRLLIAAQACLP 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  76 VLELGIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSFD--ASGFNLVIHEVAHK 153
Cdd:COG3228   81 LLGLGLDWYDGLHEILVYPGAFVVRREEQDEAGVVHEGDEVLSGEAWERGPVVLSWPDVQAGFRnpRDGHNVVIHEFAHK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 154 LDTRNGDrASGVPLIPLRE-VAGWEHDLHAAMDNIQDEIDLVGESaaSIDAYAATDPAECFAVLSEYFFSAPELFAPRFP 232
Cdd:COG3228  161 LDMLDGA-ADGVPPLHPRMdYAPWAQVFSAAYEALQRRVDAGEET--LIDPYAATNPAEFFAVASEYFFEAPELLAEKHP 237
                        250
                 ....*....|....*.
gi 697053314 233 ALWQRFCQFYQQDPLQ 248
Cdd:COG3228  238 ALYELLARFYRQDPAA 253
Peptidase_M90_mtfA cd20169
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily ...
35-242 1.18e-79

Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily includes the Mlc Titration Factor A (MtfA; also known as YeeI or DgsA anti-repressor MtfA) which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). It can cleave synthetic substrates of both carboxypeptidases and aminopeptidases, with strongest activity towards the latter. Its biologically relevant substrate has yet to be identified. Although it interacts with the transcription repressor Mlc, it does not cleave it. However, Mlc seems to activate the peptidase activity of MtfA. MtfA is related to the catalytic domain of the anthrax lethal factor which is a zinc-dependent metalloprotease, targeting mitogen-activated protein kinase kinases (MAPKKs), and resulting in apoptosis, as well as the Mop (modulation of pathogenesis) protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.


Pssm-ID: 380912  Cd Length: 208  Bit Score: 238.97  E-value: 1.18e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  35 EQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLELGIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQR 114
Cdd:cd20169    1 ERARLERLAQLFLAEKRFEGAGGLELTDEMRLLIAAQACLLVLGLGLDWYDGLREILVYPDAFVARREEEDEAGVVHEGR 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 115 VVQSGQSWQQGPIILNWLDIQDSF--DASGFNLVIHEVAHKLDTRNGDRASGVPLIPLREVAGWEHDLHAAMDNIQDEID 192
Cdd:cd20169   81 GVLGGEAWEQGPVVLSWPDVEAGFrnPGDGHNVVIHEFAHKLDMLDGDADGVPPLLSGMDARRWARVFQAAYERLRRGVS 160
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 697053314 193 LVGESAasIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQFY 242
Cdd:cd20169  161 RGRETL--IDPYAATNPAEFFAVASEYFFERPALLRREHPELYALLARFY 208
Peptidase_M90 pfam06167
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription ...
4-243 8.75e-75

Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription factor A that binds Mlc (make large colonies), itself a repressor of glucose and hence a protein important in regulation of the phosphoenolpyruvate:glucose-phosphotransferase (ptsG) system, the major glucose transporter in E.coli. Mlc is a repressor of ptsG, and MtfA is found to bind and inactivate Mlc with high affinity. The membrane-bound protein EIICBGlc encoded by the ptsG gene is the major glucose transporter in Escherichia coli. MtfA is found to be a glucose-regulated peptidase, whose activity is regulated by binding to Mlc available in the cytoplasm, which in turn has been released from EIICBGlc during times when no glucose is taken up. A physiologically relevant target for this peptidase is not yet known.


Pssm-ID: 428801  Cd Length: 243  Bit Score: 227.79  E-value: 8.75e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314    4 WPWKTNEAGRDMALP---WDDALT-IPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLEL 79
Cdd:pfam06167   2 RRWRRRRILARHPFPpawWAILERrLPFYRRLPAEERARLRELIQLFLAEKEFTGAGGLEVTDEMRLLIAAQACLPILGL 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   80 GIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSF--DASGFNLVIHEVAHKLDTR 157
Cdd:pfam06167  82 GLDYYPGLREILVYPDAFVARREVMDEDGVVHEEDEVLLGEAWERGPVILSWPDVLAGGenPGDGHNVVIHEFAHKLDML 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  158 NGDrASGVP-LIPLREVAGWEHDLHAAMDNIQDEIDlvgesAAS-IDAYAATDPAECFAVLSEYFFSAPELFAPRFPALW 235
Cdd:pfam06167 162 DGA-ADGVPpLHRGMDYRPWAKVFSAEYERLCARVD-----AGTlIDPYGATNPAEFFAVASEYFFEKPDLLQEEHPELY 235

                  ....*...
gi 697053314  236 QRFCQFYQ 243
Cdd:pfam06167 236 RQLRLFYR 243
 
Name Accession Description Interval E-value
PRK15410 PRK15410
DgsA anti-repressor MtfA; Provisional
1-260 0e+00

DgsA anti-repressor MtfA; Provisional


Pssm-ID: 185308  Cd Length: 260  Bit Score: 538.17  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   1 MIKWPWKTNEAGRDMALPWDDALTIPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLELG 80
Cdd:PRK15410   1 MIKWPWKAQEPAHQTALPWQEALSIPLLTPLTEQEQQKLVALAERFLQQKRLVPLQGLELDSLMSARIALLFCLPVLELG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  81 IEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSFDASGFNLVIHEVAHKLDTRNGD 160
Cdd:PRK15410  81 IEWLDGFHEVLIYPAPFVVDDEWEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDMRNGD 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 161 RASGVPLIPLREVAGWEHDLHAAMDNIQDEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQ 240
Cdd:PRK15410 161 RASGVPFIPLREVAGWEHDLHAAMNNIQEEIDLVGENAASIDAYAASDPAECFAVLSEYFFSAPELFAPRFPALYQRFCQ 240
                        250       260
                 ....*....|....*....|
gi 697053314 241 FYQQDPLQRLRQNEESGGHS 260
Cdd:PRK15410 241 FYRQDPLQRLHRWNDTDSFS 260
MtfA COG3228
Mlc titration factor MtfA, regulates ptsG expression [Signal transduction mechanisms];
1-248 3.44e-108

Mlc titration factor MtfA, regulates ptsG expression [Signal transduction mechanisms];


Pssm-ID: 442461  Cd Length: 253  Bit Score: 312.87  E-value: 3.44e-108
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   1 MIKWP--WKTNEAGRDMALP--WDDALT-IPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLP 75
Cdd:COG3228    1 MLRWLrrWRRRRRLARAPFPalWRAILArVPFLAGLSAEERARLRELAQLFLAEKRFEGVGGLEVTDEMRLLIAAQACLP 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  76 VLELGIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSFD--ASGFNLVIHEVAHK 153
Cdd:COG3228   81 LLGLGLDWYDGLHEILVYPGAFVVRREEQDEAGVVHEGDEVLSGEAWERGPVVLSWPDVQAGFRnpRDGHNVVIHEFAHK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 154 LDTRNGDrASGVPLIPLRE-VAGWEHDLHAAMDNIQDEIDLVGESaaSIDAYAATDPAECFAVLSEYFFSAPELFAPRFP 232
Cdd:COG3228  161 LDMLDGA-ADGVPPLHPRMdYAPWAQVFSAAYEALQRRVDAGEET--LIDPYAATNPAEFFAVASEYFFEAPELLAEKHP 237
                        250
                 ....*....|....*.
gi 697053314 233 ALWQRFCQFYQQDPLQ 248
Cdd:COG3228  238 ALYELLARFYRQDPAA 253
Peptidase_M90_mtfA cd20169
Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily ...
35-242 1.18e-79

Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase); This subfamily includes the Mlc Titration Factor A (MtfA; also known as YeeI or DgsA anti-repressor MtfA) which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). It can cleave synthetic substrates of both carboxypeptidases and aminopeptidases, with strongest activity towards the latter. Its biologically relevant substrate has yet to be identified. Although it interacts with the transcription repressor Mlc, it does not cleave it. However, Mlc seems to activate the peptidase activity of MtfA. MtfA is related to the catalytic domain of the anthrax lethal factor which is a zinc-dependent metalloprotease, targeting mitogen-activated protein kinase kinases (MAPKKs), and resulting in apoptosis, as well as the Mop (modulation of pathogenesis) protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.


Pssm-ID: 380912  Cd Length: 208  Bit Score: 238.97  E-value: 1.18e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  35 EQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLELGIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQR 114
Cdd:cd20169    1 ERARLERLAQLFLAEKRFEGAGGLELTDEMRLLIAAQACLLVLGLGLDWYDGLREILVYPDAFVARREEEDEAGVVHEGR 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 115 VVQSGQSWQQGPIILNWLDIQDSF--DASGFNLVIHEVAHKLDTRNGDRASGVPLIPLREVAGWEHDLHAAMDNIQDEID 192
Cdd:cd20169   81 GVLGGEAWEQGPVVLSWPDVEAGFrnPGDGHNVVIHEFAHKLDMLDGDADGVPPLLSGMDARRWARVFQAAYERLRRGVS 160
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 697053314 193 LVGESAasIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQFY 242
Cdd:cd20169  161 RGRETL--IDPYAATNPAEFFAVASEYFFERPALLRREHPELYALLARFY 208
Peptidase_M90 pfam06167
Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription ...
4-243 8.75e-75

Glucose-regulated metallo-peptidase M90; MtfA (earlier known as YeeI) is a transcription factor A that binds Mlc (make large colonies), itself a repressor of glucose and hence a protein important in regulation of the phosphoenolpyruvate:glucose-phosphotransferase (ptsG) system, the major glucose transporter in E.coli. Mlc is a repressor of ptsG, and MtfA is found to bind and inactivate Mlc with high affinity. The membrane-bound protein EIICBGlc encoded by the ptsG gene is the major glucose transporter in Escherichia coli. MtfA is found to be a glucose-regulated peptidase, whose activity is regulated by binding to Mlc available in the cytoplasm, which in turn has been released from EIICBGlc during times when no glucose is taken up. A physiologically relevant target for this peptidase is not yet known.


Pssm-ID: 428801  Cd Length: 243  Bit Score: 227.79  E-value: 8.75e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314    4 WPWKTNEAGRDMALP---WDDALT-IPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIALLFCLPVLEL 79
Cdd:pfam06167   2 RRWRRRRILARHPFPpawWAILERrLPFYRRLPAEERARLRELIQLFLAEKEFTGAGGLEVTDEMRLLIAAQACLPILGL 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314   80 GIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSGQSWQQGPIILNWLDIQDSF--DASGFNLVIHEVAHKLDTR 157
Cdd:pfam06167  82 GLDYYPGLREILVYPDAFVARREVMDEDGVVHEEDEVLLGEAWERGPVILSWPDVLAGGenPGDGHNVVIHEFAHKLDML 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  158 NGDrASGVP-LIPLREVAGWEHDLHAAMDNIQDEIDlvgesAAS-IDAYAATDPAECFAVLSEYFFSAPELFAPRFPALW 235
Cdd:pfam06167 162 DGA-ADGVPpLHRGMDYRPWAKVFSAEYERLCARVD-----AGTlIDPYGATNPAEFFAVASEYFFEKPDLLQEEHPELY 235

                  ....*...
gi 697053314  236 QRFCQFYQ 243
Cdd:pfam06167 236 RQLRLFYR 243
Peptidase_M90-like cd20170
uncharacterized M90 peptidase family-like proteins; This subfamily contains uncharacterized ...
25-240 2.36e-17

uncharacterized M90 peptidase family-like proteins; This subfamily contains uncharacterized M90 peptidase-like domains, similar to the Mlc Titration Factor A (MtfA) peptidase from Escherichia coli, also known as the YeeI gene product, which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). E. coli MtfA has been shown to have aminopeptidase activity with the presence of a single zinc ion in the active site ligated by two histidines in an HEXXH motif. MtfA is related to the catalytic domain of the anthrax lethal factor and the Mop protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.


Pssm-ID: 380913  Cd Length: 210  Bit Score: 77.92  E-value: 2.36e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  25 IPVLANLKPDEQAKLVQLADRFLQQKRLVPLQGFELDPLKNARIA-----LLFCLPVLelgieWLDGFHEVLIYPAPFIV 99
Cdd:cd20170    6 FPYYNKLSPKQKKRFEHRVARFINSKKFIGREGLEVTEEMKVLIAatavqLTFGLRNY-----LLSHFDTILIYPDAYYS 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 100 DDEWEDDIGLVhNQRVvqsgqswqqGPIILNWLD----IQDSFDasGFNLVIHEVAH--KLDTRNGDRASgvpliplrev 173
Cdd:cd20170   81 TINKQYHKGEV-NPRL---------KAIVLSWKDflegYAIPND--GLNLGLHEMAHalHLENLISNDES---------- 138
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 697053314 174 agwEHDLHAAMDNIQ----DEIDLVGESAASI-DAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFCQ 240
Cdd:cd20170  139 ---AFFFRKGFKKFKklakDEFEKIKLGESSYfRDYAFTNIHEFFAVAVENFFETPEEFKAEFPELYEALKK 207
Peptidase_M90-like cd20167
M90 peptidase is a zinc-metallopeptidase; The M90 peptidase family includes the MtfA (Mlc ...
35-236 5.33e-10

M90 peptidase is a zinc-metallopeptidase; The M90 peptidase family includes the MtfA (Mlc Titration Factor A) peptidase from Escherichia coli, also known as the YeeI gene product, which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). E. coli MtfA has been shown to have aminopeptidase activity with the presence of a single zinc ion in the active site ligated by two histidines in a HEXXH motif. This family also includes uncharacterized proteins similar to MtfA peptidase.


Pssm-ID: 380911  Cd Length: 208  Bit Score: 57.91  E-value: 5.33e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314  35 EQAKLVQLADRFLQQKRLVPlQGFEL--DPLK----NARIALLFCLPVLelgieWLDGFHEVLIYPAPFIVDDEWEDDIG 108
Cdd:cd20167    1 EQHKLTQ*AARFLQQKRLVA-LQGLEltPLHQaria*LFCLPVLELGIE-----WLDGFHEVLIYPAPFIVDDEWEDDIG 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 697053314 109 LVHNQRVVQSGQSWQQGPIILNWLDIQDSFDASGFNLVIHEVAHKLDTRNGDRASGVPLIPLREVAGWEHDLHAAMDNIQ 188
Cdd:cd20167   75 LVHNQRVVQSGQSWQQGPVVLNWLDIQDSFDASGFNLVVHEVAHKLDTRNGDRASGVPLIPLREVAGWEHDLHAA*NNIQ 154
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 697053314 189 DEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQ 236
Cdd:cd20167  155 DEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQ 202
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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