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Conserved domains on  [gi|723054953|ref|WP_033550757|]
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MULTISPECIES: type IV secretion system protein [Enterobacteriaceae]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
VirB5_like super family cl02193
VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog ...
 21-201 6.11e-25

VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog encoded by the pKM101 plasmid, and similar proteins. VirB5 is one of 11 conserved proteins (VirB1-VirB11) in Agrobacterium tumefaciens, the causative agent of crown gall disease, that span the inner and the outer membrane, and is involved in type IV DNA secretion systems (T4SS) which mediate the translocation of virulence factors (proteins and/or DNA) from Gram-negative bacteria into eukaryotic cells. VirB5 assembles extracellular pili by interacting with several essential proteins. VirB2-VirB5 complex formation precedes incorporation into pili; it depends on the inner membrane protein VirB4 to interact directly with and stabilize VirB8 in order for VirB5 to bind to VirB8 and VirB10. Mutagenesis studies show that VirB5 proteins participate in protein-protein interactions important for pilus assembly and function.


The actual alignment was detected with superfamily member pfam07996:

Pssm-ID: 445702 [Multi-domain]  Cd Length: 189  Bit Score: 97.03  E-value: 6.11e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   21 GIPVaIDASpewAIEATrwTERLKQWSETARHYQSQIQAYKDQLATATGIRNIAAFTNE--LSN-LQSELANIYKQGNSY 97
Cdd:pfam07996   1 GIPV-IDAA---AIAQA--IAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNpaLRNyLPADWQDIYDLVKSG 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   98 ISDftsnpeGALSSQAKNLFSKYGAFDMCNTGieSSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQSLSSRIESSKD 177
Cdd:pfam07996  75 GSY------GSLSSAAQSLRDANKLYDVCTDD--DRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATD 146

                         170       180
                  ....*....|....*....|....
gi 723054953  178 MKESQDLANALQAQSLKIQAIKMQ 201
Cdd:pfam07996 147 PKAIADLQARIQAEQAMLQNEQTR 170
 
Name Accession Description Interval E-value
T4SS pfam07996
Type IV secretion system proteins; Members of this family are components of the type IV ...
 21-201 6.11e-25

Type IV secretion system proteins; Members of this family are components of the type IV secretion system. They mediate intracellular transfer of macromolecules via a mechanism ancestrally related to that of bacterial conjugation machineries.


Pssm-ID: 429777 [Multi-domain]  Cd Length: 189  Bit Score: 97.03  E-value: 6.11e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   21 GIPVaIDASpewAIEATrwTERLKQWSETARHYQSQIQAYKDQLATATGIRNIAAFTNE--LSN-LQSELANIYKQGNSY 97
Cdd:pfam07996   1 GIPV-IDAA---AIAQA--IAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNpaLRNyLPADWQDIYDLVKSG 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   98 ISDftsnpeGALSSQAKNLFSKYGAFDMCNTGieSSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQSLSSRIESSKD 177
Cdd:pfam07996  75 GSY------GSLSSAAQSLRDANKLYDVCTDD--DRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATD 146

                         170       180
                  ....*....|....*....|....
gi 723054953  178 MKESQDLANALQAQSLKIQAIKMQ 201
Cdd:pfam07996 147 PKAIADLQARIQAEQAMLQNEQTR 170
VirB5_like cd14262
VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog ...
 41-201 1.70e-08

VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog encoded by the pKM101 plasmid, and similar proteins. VirB5 is one of 11 conserved proteins (VirB1-VirB11) in Agrobacterium tumefaciens, the causative agent of crown gall disease, that span the inner and the outer membrane, and is involved in type IV DNA secretion systems (T4SS) which mediate the translocation of virulence factors (proteins and/or DNA) from Gram-negative bacteria into eukaryotic cells. VirB5 assembles extracellular pili by interacting with several essential proteins. VirB2-VirB5 complex formation precedes incorporation into pili; it depends on the inner membrane protein VirB4 to interact directly with and stabilize VirB8 in order for VirB5 to bind to VirB8 and VirB10. Mutagenesis studies show that VirB5 proteins participate in protein-protein interactions important for pilus assembly and function.


Pssm-ID: 271354 [Multi-domain]  Cd Length: 173  Bit Score: 52.35  E-value: 1.70e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953  41 ERLKQWSETARHYQSQIQAYKDQLATATGIRNIAAF---TNELSNLQSELANIYKQGNSYisdftsnpEGALSSQAKNLF 117
Cdd:cd14262    1 QQLAQLKQQLEQLKQQLEQAKKQYDSITGSRGLGDLlnnPNLRSLLPLDPQSIYSAAKGG--------GSSGSSGSLAAI 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953 118 SKYGAFDMCNTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQSLSSRIESSKDMKESQDLANALQAQSLKIQ- 196
Cdd:cd14262   73 ISQNEEERRLDSVGEAREAIDKRSQYAAQDKAVSLQAYEEAEQRLKQIQSLLQQINTTQDPKAIADLQARIQAEQAMIQn 152


                 ....*.
gi 723054953 197 -AIKMQ 201
Cdd:cd14262  153 eQTKLQ 158
 
Name Accession Description Interval E-value
T4SS pfam07996
Type IV secretion system proteins; Members of this family are components of the type IV ...
 21-201 6.11e-25

Type IV secretion system proteins; Members of this family are components of the type IV secretion system. They mediate intracellular transfer of macromolecules via a mechanism ancestrally related to that of bacterial conjugation machineries.


Pssm-ID: 429777 [Multi-domain]  Cd Length: 189  Bit Score: 97.03  E-value: 6.11e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   21 GIPVaIDASpewAIEATrwTERLKQWSETARHYQSQIQAYKDQLATATGIRNIAAFTNE--LSN-LQSELANIYKQGNSY 97
Cdd:pfam07996   1 GIPV-IDAA---AIAQA--IAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNpaLRNyLPADWQDIYDLVKSG 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953   98 ISDftsnpeGALSSQAKNLFSKYGAFDMCNTGieSSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQSLSSRIESSKD 177
Cdd:pfam07996  75 GSY------GSLSSAAQSLRDANKLYDVCTDD--DRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATD 146

                         170       180
                  ....*....|....*....|....
gi 723054953  178 MKESQDLANALQAQSLKIQAIKMQ 201
Cdd:pfam07996 147 PKAIADLQARIQAEQAMLQNEQTR 170
VirB5_like cd14262
VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog ...
 41-201 1.70e-08

VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog encoded by the pKM101 plasmid, and similar proteins. VirB5 is one of 11 conserved proteins (VirB1-VirB11) in Agrobacterium tumefaciens, the causative agent of crown gall disease, that span the inner and the outer membrane, and is involved in type IV DNA secretion systems (T4SS) which mediate the translocation of virulence factors (proteins and/or DNA) from Gram-negative bacteria into eukaryotic cells. VirB5 assembles extracellular pili by interacting with several essential proteins. VirB2-VirB5 complex formation precedes incorporation into pili; it depends on the inner membrane protein VirB4 to interact directly with and stabilize VirB8 in order for VirB5 to bind to VirB8 and VirB10. Mutagenesis studies show that VirB5 proteins participate in protein-protein interactions important for pilus assembly and function.


Pssm-ID: 271354 [Multi-domain]  Cd Length: 173  Bit Score: 52.35  E-value: 1.70e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953  41 ERLKQWSETARHYQSQIQAYKDQLATATGIRNIAAF---TNELSNLQSELANIYKQGNSYisdftsnpEGALSSQAKNLF 117
Cdd:cd14262    1 QQLAQLKQQLEQLKQQLEQAKKQYDSITGSRGLGDLlnnPNLRSLLPLDPQSIYSAAKGG--------GSSGSSGSLAAI 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 723054953 118 SKYGAFDMCNTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQSLSSRIESSKDMKESQDLANALQAQSLKIQ- 196
Cdd:cd14262   73 ISQNEEERRLDSVGEAREAIDKRSQYAAQDKAVSLQAYEEAEQRLKQIQSLLQQINTTQDPKAIADLQARIQAEQAMIQn 152


                 ....*.
gi 723054953 197 -AIKMQ 201
Cdd:cd14262  153 eQTKLQ 158
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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