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Conserved domains on  [gi|727184181|ref|WP_033646365|]
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MULTISPECIES: GNAT family N-acetyltransferase [Serratia]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-124 3.64e-13

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 62.70  E-value: 3.64e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIAL--------DSVAQNAPQRAAQIRAWCAQ-----GICFLAEEQGMVMGYGVLHYHFFGCGF--- 64
Cdd:COG1247    2 MTIRPATPEDAPAIAAIyneaiaegTATFETEPPSEEEREAWFAAilapgRPVLVAEEDGEVVGFASLGPFRPRPAYrgt 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 727184181  65 -IEMLMVGERYRRRGVGQALIAVLKSHCR---HPKLFTSTNRSNLPMQRLLLNAGFVASGQIDN 124
Cdd:COG1247   82 aEESIYVDPDARGRGIGRALLEALIERARargYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-124 3.64e-13

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 62.70  E-value: 3.64e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIAL--------DSVAQNAPQRAAQIRAWCAQ-----GICFLAEEQGMVMGYGVLHYHFFGCGF--- 64
Cdd:COG1247    2 MTIRPATPEDAPAIAAIyneaiaegTATFETEPPSEEEREAWFAAilapgRPVLVAEEDGEVVGFASLGPFRPRPAYrgt 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 727184181  65 -IEMLMVGERYRRRGVGQALIAVLKSHCR---HPKLFTSTNRSNLPMQRLLLNAGFVASGQIDN 124
Cdd:COG1247   82 aEESIYVDPDARGRGIGRALLEALIERARargYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 40-116 3.93e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 56.37  E-value: 3.93e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   40 CFLAEEQGMVMGYGVLH--YHFFGCGFIEMLMVGERYRRRGVGQALIAVLKSHCRH---PKLFTSTNRSNLPMQRLLLNA 114
Cdd:pfam00583  35 FFVAEEDGELVGFASLSiiDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARErgcERIFLEVAADNLAAIALYEKL 114


                  ..
gi 727184181  115 GF 116
Cdd:pfam00583 115 GF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 40-92 1.11e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 43.42  E-value: 1.11e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 727184181  40 CFLAEEQGMVMGYGVLHYHFF--GCGFIEMLMVGERYRRRGVGQALIAVLKSHCR 92
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSggDTAYIGDLAVLPEYRGKGIGSALLEAAEEEAR 55
PRK07757 PRK07757
N-acetyltransferase;
1-84 4.17e-04

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 38.25  E-value: 4.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRlaaetdaaaliALDSVAQNapqraaqIRAWcaqgicFLAEEQGMVMGYGVLHYHFFGCGFIEMLMVGERYRRRGVG 80
Cdd:PRK07757  28 MLPR-----------SLDELYEN-------IRDF------YVAEEEGEIVGCCALHILWEDLAEIRSLAVSEDYRGQGIG 83


                 ....
gi 727184181  81 QALI 84
Cdd:PRK07757  84 RMLV 87
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-124 3.64e-13

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 62.70  E-value: 3.64e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIAL--------DSVAQNAPQRAAQIRAWCAQ-----GICFLAEEQGMVMGYGVLHYHFFGCGF--- 64
Cdd:COG1247    2 MTIRPATPEDAPAIAAIyneaiaegTATFETEPPSEEEREAWFAAilapgRPVLVAEEDGEVVGFASLGPFRPRPAYrgt 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 727184181  65 -IEMLMVGERYRRRGVGQALIAVLKSHCR---HPKLFTSTNRSNLPMQRLLLNAGFVASGQIDN 124
Cdd:COG1247   82 aEESIYVDPDARGRGIGRALLEALIERARargYRRLVAVVLADNEASIALYEKLGFEEVGTLPE 145
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 40-116 3.93e-11

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 56.37  E-value: 3.93e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   40 CFLAEEQGMVMGYGVLH--YHFFGCGFIEMLMVGERYRRRGVGQALIAVLKSHCRH---PKLFTSTNRSNLPMQRLLLNA 114
Cdd:pfam00583  35 FFVAEEDGELVGFASLSiiDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARErgcERIFLEVAADNLAAIALYEKL 114


                  ..
gi 727184181  115 GF 116
Cdd:pfam00583 115 GF 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 1-117 1.80e-10

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 55.00  E-value: 1.80e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIALdsvaqnapqraaqIRAWCAQ---GICFLAEEQGMVMGYGVLHYHFFGCGFIEMLMVGERYRRR 77
Cdd:COG1246    1 MTIRPATPDDVPAILEL-------------IRPYALEeeiGEFWVAEEDGEIVGCAALHPLDEDLAELRSLAVHPDYRGR 67

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 727184181  78 GVGQALIAVLKSHCRH---PKLFTSTNRsnlPMQRLLLNAGFV 117
Cdd:COG1246   68 GIGRRLLEALLAEARElglKRLFLLTTS---AAIHFYEKLGFE 107
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-136 4.27e-10

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 53.94  E-value: 4.27e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   3 VRLAAETDAAALIALDSVA---QNAPQRAAQIRAWCAQGICFLAEEQGMVMGYGVLH----YHFFGCGFIEMLMVGERYR 75
Cdd:COG3153    1 IRPATPEDAEAIAALLRAAfgpGREAELVDRLREDPAAGLSLVAEDDGEIVGHVALSpvdiDGEGPALLLGPLAVDPEYR 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 727184181  76 RRGVGQALIAVLKSHCR---HPKLFTSTNRSNLPM-QRLllnaGFVASGQIDnlDDGDPEQVFFI 136
Cdd:COG3153   81 GQGIGRALMRAALEAARergARAVVLLGDPSLLPFyERF----GFRPAGELG--LTLGPDEVFLA 139
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 51-124 6.82e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 49.65  E-value: 6.82e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 727184181  51 GYGVLHYHFFG-CGFIEMLMVGERYRRRGVGQALIAVLKSHCRH---PKLFTSTNRSNLPMQRLLLNAGFVASGQIDN 124
Cdd:COG0456    1 GFALLGLVDGGdEAEIEDLAVDPEYRGRGIGRALLEAALERARErgaRRLRLEVREDNEAAIALYEKLGFEEVGERPN 78
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 1-122 9.79e-08

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 47.74  E-value: 9.79e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIALDSVAQNAPQRAAQIRawcaQGICFLAEEQGMVMGYGVLHYHFFGCGFIEMLMVGERYRRRGVG 80
Cdd:COG0454    1 MSIRKATPEDINFILLIEALDAELKAMEGSLA----GAEFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVLPEYRGKGIG 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 727184181  81 QALIAVLKSHCRH--PKLFTS-TNRSNLPMQRLLLNAGFVASGQI 122
Cdd:COG0454   77 KALLEALLEWARErgCTALELdTLDGNPAAIRFYERLGFKEIERY 121
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 40-92 1.11e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 43.42  E-value: 1.11e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 727184181  40 CFLAEEQGMVMGYGVLHYHFF--GCGFIEMLMVGERYRRRGVGQALIAVLKSHCR 92
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSggDTAYIGDLAVLPEYRGKGIGSALLEAAEEEAR 55
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 38-117 1.30e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 43.60  E-value: 1.30e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   38 GICFLAEEQGMVMGYGVLHYHFFGCGFIE-MLMVGERYRRRGVGQALIAVLKSHCRHPKLFTSTNRSNLPMQRLLLNAGF 116
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPLDDEGALAElRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKLGF 82


                  .
gi 727184181  117 V 117
Cdd:pfam13508  83 E 83
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 1-124 1.39e-06

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 44.99  E-value: 1.39e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRLAAETDAAALIALDSVAQ------NAPQRAAQIRAWCAQ--------GICFLA---EEQGMVMGYGVLHYHFFGCG 63
Cdd:COG1670    8 LRLRPLRPEDAEALAELLNDPEvarylpGPPYSLEEARAWLERlladwadgGALPFAiedKEDGELIGVVGLYDIDRANR 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 727184181  64 FIEM-LMVGERYRRRGVG----QALIAVLKSHCRHPKLFTSTNRSNLPMQRLLLNAGFVASGQIDN 124
Cdd:COG1670   88 SAEIgYWLAPAYWGKGYAtealRALLDYAFEELGLHRVEAEVDPDNTASIRVLEKLGFRLEGTLRD 153
Acetyltransf_3 pfam13302
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 2-117 5.95e-05

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 379112 [Multi-domain]  Cd Length: 139  Bit Score: 40.41  E-value: 5.95e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181    2 LVRLAAETDAAALIALDS------VAQNAPQRAAQIRAWCA---------QGICFLAEEQG-MVMGYGVLHYHFFGCGFI 65
Cdd:pfam13302   3 LLRPLTEEDAEALFELLSdpevmrYGVPWPLTLEEAREWLAriwaadeaeRGYGWAIELKDtGFIGSIGLYDIDGEPERA 82

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 727184181   66 EM-LMVGERYRRRGVG----QALIAVLKSHCRHPKLFTSTNRSNLPMQRLLLNAGFV 117
Cdd:pfam13302  83 ELgYWLGPDYWGKGYAteavRALLEYAFEELGLPRLVARIDPENTASRRVLEKLGFK 139
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
41-128 3.55e-04

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 37.86  E-value: 3.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181  41 FLAEEQGMVMGYGVLHYHFFGCGFIEMLMVGERYRRRGVGQALIAVLKSHCRhpklftstnrsNLPMQRLLLNA------ 114
Cdd:COG2153   37 LLAYDDGELVATARLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEAR-----------ERGARRIVLSAqahavg 105

                         90
                 ....*....|....*....
gi 727184181 115 -----GFVASGQIDnLDDG 128
Cdd:COG2153  106 fyeklGFVPVGEEF-LEAG 123
PRK07757 PRK07757
N-acetyltransferase;
1-84 4.17e-04

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 38.25  E-value: 4.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   1 MLVRlaaetdaaaliALDSVAQNapqraaqIRAWcaqgicFLAEEQGMVMGYGVLHYHFFGCGFIEMLMVGERYRRRGVG 80
Cdd:PRK07757  28 MLPR-----------SLDELYEN-------IRDF------YVAEEEGEIVGCCALHILWEDLAEIRSLAVSEDYRGQGIG 83


                 ....
gi 727184181  81 QALI 84
Cdd:PRK07757  84 RMLV 87
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
61-122 6.23e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 36.43  E-value: 6.23e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 727184181  61 GCGFIEMLMVGERYRRRGVGQALIAVLKSHCR---HPKLFTSTNRSNLPMQRLLLNAGFVASGQI 122
Cdd:COG3393   14 GVAEISGVYTHPEYRGRGLASALVAALAREALargARTPFLYVDADNPAARRLYERLGFRPVGEY 78
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 28-137 3.51e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 35.32  E-value: 3.51e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727184181   28 AAQIRAWCAQGICFL--AEEQGMVMGYGVLHyhffGCGFIEMLMVGERYRRRGVGQALIAVLKSHCR--HPKLFTSTNRS 103
Cdd:pfam13673  19 PEALRERIDQGEYFFfvAFEGGQIVGVIALR----DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEkdGIKLSELTVNA 94

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 727184181  104 NLPMQRLLLNAGFVASGqidnlddgdPEQVF----FIP 137
Cdd:pfam13673  95 SPYAVPFYEKLGFRATG---------PEQEFngirFVP 123
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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