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Conserved domains on  [gi|727824003|ref|WP_033907186|]
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MULTISPECIES: thiaminase II [Vibrio]

Protein Classification

TenA family protein( domain architecture ID 14443675)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-217 1.42e-103

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


:

Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 297.50  E-value: 1.42e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  13 DWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDsEIAHHVTYCEQW 92
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCAEW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  93 GLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTVIEGNPYASWINLYGGEEFQS 172
Cdd:cd19367   81 GISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQE 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 727824003 173 GVAKGAEHFNQLLAEIdINSQRGQNLIQVFKTATRMEVAFWQQGL 217
Cdd:cd19367  161 AVREGIELLEKLAAER-PSPARLEELVKIFATATRLEIGFWDMGL 204
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-217 1.42e-103

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 297.50  E-value: 1.42e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  13 DWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDsEIAHHVTYCEQW 92
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCAEW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  93 GLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTVIEGNPYASWINLYGGEEFQS 172
Cdd:cd19367   81 GISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQE 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 727824003 173 GVAKGAEHFNQLLAEIdINSQRGQNLIQVFKTATRMEVAFWQQGL 217
Cdd:cd19367  161 AVREGIELLEKLAAER-PSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 3-221 2.62e-84

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 249.41  E-value: 2.62e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   3 YQDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEI 82
Cdd:COG0819    4 SERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVEL 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  83 AHHVTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtvIEGNPYASWI 162
Cdd:COG0819   84 ALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPL--PPDHPYAEWI 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 727824003 163 NLYGGEEFQSGVAKGAEHFNQLLAEIDinSQRGQNLIQVFKTATRMEVAFWQQGLNAQQ 221
Cdd:COG0819  162 ETYASEEFQELVEWLIALLDRLAATAS--EAERERLEEAFRTASRLEYAFWDMAYRLER 218
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-219 9.82e-43

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 143.27  E-value: 9.82e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   94 LTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEdENTVIEGNPYASWINLYGGEEFQSG 173
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGE-KIRALEGPEYYEWVKTYASEYFRAA 166

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 727824003  174 VAKGAEHFNQLLAeiDINSQRGQNLIQVFKTATRMEVAFWQQGLNA 219
Cdd:pfam03070 167 VEEAKRLLDRLLA--YVSDEQLEELQEIFLKALEFEYSFWTMALDA 210
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 14-213 9.04e-37

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 128.08  E-value: 9.04e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:TIGR04306   5 WDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAEVLG 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   94 LTESD-LENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtVIEGNPYASWINLYGGEEFQS 172
Cdd:TIGR04306  85 ISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHT-APEHSVYHKWIETYSSSWFRE 163

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 727824003  173 GVAKGAEHFNQlLAEIDINSQRGQnLIQVFKTATRMEVAFW 213
Cdd:TIGR04306 164 AVNEGENLLNH-LAETSSPEELEK-LKTIFAESCEYEYNFW 202
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
6-216 1.26e-22

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 95.24  E-value: 1.26e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   6 LIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHH 85
Cdd:PRK14713 324 LWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELH 403

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  86 vtycEQW-GLTESDLENEPEdfgTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLiEDENTVIEGNPYASWINL 164
Cdd:PRK14713 404 ----RSWlGDRDADTAPSPV---TLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAEL-HARAGNPDDHPYAEWLQT 475

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 727824003 165 YGGEEFQSGVAKgAEHFNQLLAEIDINSQRgQNLIQVFKTATRMEVAFWQQG 216
Cdd:PRK14713 476 YADPEFAAATRR-AIAFVDRAFRAASPAER-AAMARAFLTACRYELEFFDQA 525
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-217 1.42e-103

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 297.50  E-value: 1.42e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  13 DWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDsEIAHHVTYCEQW 92
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCAEW 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  93 GLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTVIEGNPYASWINLYGGEEFQS 172
Cdd:cd19367   81 GISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQE 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 727824003 173 GVAKGAEHFNQLLAEIdINSQRGQNLIQVFKTATRMEVAFWQQGL 217
Cdd:cd19367  161 AVREGIELLEKLAAER-PSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 3-221 2.62e-84

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 249.41  E-value: 2.62e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   3 YQDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEI 82
Cdd:COG0819    4 SERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVEL 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  83 AHHVTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtvIEGNPYASWI 162
Cdd:COG0819   84 ALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPL--PPDHPYAEWI 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 727824003 163 NLYGGEEFQSGVAKGAEHFNQLLAEIDinSQRGQNLIQVFKTATRMEVAFWQQGLNAQQ 221
Cdd:COG0819  162 ETYASEEFQELVEWLIALLDRLAATAS--EAERERLEEAFRTASRLEYAFWDMAYRLER 218
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 14-213 1.01e-54

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 173.90  E-value: 1.01e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:cd19366   13 WEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEMDLHRQYAAEFG 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  94 LTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTvIEGNPYASWINLYGGEEFQSG 173
Cdd:cd19366   93 ITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGA-LEHNPYREWIEMYSSDEFTEL 171

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 727824003 174 VAKGAEHFNQLLAeiDINSQRGQNLIQVFKTATRMEVAFW 213
Cdd:cd19366  172 ADWLIDLLDELAE--GKSEAELERLEEIFLTSSRYEYMFW 209
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 14-214 4.50e-51

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 164.32  E-value: 4.50e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:cd19369    3 WEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKEFG 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  94 LTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTVIEGNPYASWINLYGGEEFQSG 173
Cdd:cd19369   83 LTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDNLESNPYKDWIEDYASEEYAEY 162

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 727824003 174 VAKGAEHFNQLLAEIDINSQrgQNLIQVFKTATRMEVAFWQ 214
Cdd:cd19369  163 CKEWIDFADRLCENLSEEEK--EKLKEIFRKASLYELKFWD 201
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 4-213 3.95e-45

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 149.27  E-value: 3.95e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   4 QDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIA 83
Cdd:cd19361    3 ERLYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEEMD 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  84 HHVTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTVIEGNPYASWIN 163
Cdd:cd19361   83 IHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERYPAALEHEFYGEWVK 162

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 727824003 164 LYGGEEFQSGVAKGAEHFNQLLAeiDINSQRGQNLIQVFKTATRMEVAFW 213
Cdd:cd19361  163 GYSSEEYAEANQELIDLLDRLTE--DISEEQIEKLEEIFVNCSRYELKFW 210
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-219 9.82e-43

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 143.27  E-value: 9.82e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   94 LTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEdENTVIEGNPYASWINLYGGEEFQSG 173
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGE-KIRALEGPEYYEWVKTYASEYFRAA 166

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 727824003  174 VAKGAEHFNQLLAeiDINSQRGQNLIQVFKTATRMEVAFWQQGLNA 219
Cdd:pfam03070 167 VEEAKRLLDRLLA--YVSDEQLEELQEIFLKALEFEYSFWTMALDA 210
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 13-215 1.45e-39

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 134.79  E-value: 1.45e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  13 DWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDsEIAHHVTYCEQW 92
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFLAELINVLDD-ELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  93 GLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtviEGNPYASWINLYGGEEFQS 172
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASLP---EHPPYRFWIDFYASDEYQE 156

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 727824003 173 GVAKGAEHFNQLLAEIDINSQRgqnLIQVFKTATRMEVAFWQQ 215
Cdd:cd16099  157 LVEELLQLLDQLAAAGEEEKEE---LKEIFLTSLRYELMFWDA 196
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 4-215 7.70e-39

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 133.02  E-value: 7.70e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   4 QDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIA 83
Cdd:cd19365    2 EALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVERE 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  84 HHVTYCEQWGLTEsDLENEPedfGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEdenTVIEGNPYASWIN 163
Cdd:cd19365   82 LHRSFLGEFGIDA-AAEPSP---VTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAA---AASPNHPYQDWID 154

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 727824003 164 LYGGEEFQSGVAKGAEHFNQLLAEIDiNSQRGQnLIQVFKTATRMEVAFWQQ 215
Cdd:cd19365  155 TYSDEEFAEAVRRAIAIVDRLAAEAS-PEERAR-MLEAFLRASRLEWMFWDA 204
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 14-213 9.04e-37

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 128.08  E-value: 9.04e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:TIGR04306   5 WDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAEVLG 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   94 LTESD-LENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtVIEGNPYASWINLYGGEEFQS 172
Cdd:TIGR04306  85 ISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKHT-APEHSVYHKWIETYSSSWFRE 163

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 727824003  173 GVAKGAEHFNQlLAEIDINSQRGQnLIQVFKTATRMEVAFW 213
Cdd:TIGR04306 164 AVNEGENLLNH-LAETSSPEELEK-LKTIFAESCEYEYNFW 202
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 2-212 8.26e-34

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 120.42  E-value: 8.26e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   2 KYQDLIDACRqdwqeytEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRralpSVHALLDS- 80
Cdd:cd19368    8 KNRDEALFAL-------YHPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKK----AIRELRKGv 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  81 --EIAHHVTYCEQWGLtesDLENEPE-DFGTVAYTRYVLDAGMTG--DLVDLYAALAPCSIGYAVIGKMLIEDENTViEG 155
Cdd:cd19368   77 leELKLHDSYAEEWGV---DLPKEVTpDPATRKYTDFLLATASGKvkVAAYTLAAMAPCMRLYAFLGQELARALDDT-ED 152

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 727824003 156 NPYASWINLYGGEEFQSgVAKGAEhfnQLLAEIDINSQRG--QNLIQVFKTATRMEVAF 212
Cdd:cd19368  153 HPYKKWIDTYSSQEFEA-LALQLE---DLLDKLSASLTGEelEALEKLYRRAMKLEVEF 207
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 4-215 2.36e-33

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 119.23  E-value: 2.36e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   4 QDLIDACRQdwqeyteHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIA 83
Cdd:cd19360   10 QPILEAIYA-------HPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGESH 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  84 HHVTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEdENTVIEGNPYASWIN 163
Cdd:cd19360   83 AHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIE-EGKPTPDNPFYEWID 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 727824003 164 LYGGEEFQSGVAKGAEHFNQlLAEIDINSQRGQnLIQVFKTATRMEVAFWQQ 215
Cdd:cd19360  162 FYADDEMDGLTDQLFARLDR-LAEKASEEERER-AKQAFLKSCQLEWRFWEM 211
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 14-214 6.06e-31

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 113.05  E-value: 6.06e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  14 WQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQWG 93
Cdd:cd19364   13 WQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGELALRETFFKELG 92

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  94 LTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEdentVIEGNP-YASWINLYGGEEFQS 172
Cdd:cd19364   93 ITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLAD----AGSPVPlYQRWIDTYASDEFTE 168

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 727824003 173 GVAKGAEHFNQLLAEIDINSQrgQNLIQVFKTATRMEVAFWQ 214
Cdd:cd19364  169 SVQQQIDLVDRLAEEASEEER--EKMKQAFLISSYYELQFWE 208
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 4-214 4.19e-23

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 92.39  E-value: 4.19e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   4 QDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIA 83
Cdd:cd19363    3 GKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEFA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  84 HHVTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVI----GKMLIEDENTViegnpYA 159
Cdd:cd19363   83 NYEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIaeyhKDKLSENPNDI-----YR 157

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 727824003 160 SWINLYGGEEFQSGVAKGAEHFNQLLAEIDINSqrgqnLIQVFKTATRMEVAFWQ 214
Cdd:cd19363  158 DWASVYLSKEYKELVERLRRIVDKYGEGEPFEK-----LKRIFKTASKYEYMFWD 207
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
6-216 1.26e-22

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 95.24  E-value: 1.26e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   6 LIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHH 85
Cdd:PRK14713 324 LWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELH 403

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  86 vtycEQW-GLTESDLENEPEdfgTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLiEDENTVIEGNPYASWINL 164
Cdd:PRK14713 404 ----RSWlGDRDADTAPSPV---TLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAEL-HARAGNPDDHPYAEWLQT 475

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 727824003 165 YGGEEFQSGVAKgAEHFNQLLAEIDINSQRgQNLIQVFKTATRMEVAFWQQG 216
Cdd:PRK14713 476 YADPEFAAATRR-AIAFVDRAFRAASPAER-AAMARAFLTACRYELEFFDQA 525
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 6-213 8.69e-22

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 88.78  E-value: 8.69e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   6 LIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHH 85
Cdd:cd19358    5 LRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARFLGFLANDENDYF 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  86 VTYCEQWGLTESDLENEPEDFGTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKmliEDENTVIEGNPYASWINLY 165
Cdd:cd19358   85 ERAFAALGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWAS---RAAAAAPLRFKHQEWIDLH 161

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 727824003 166 GGEEFQSGVAkgaehFnqLLAEID---INSQRGQNLIQVFKTATRMEVAFW 213
Cdd:cd19358  162 SGPEFEAWVD-----F--LRDEVDrvgPTEEERERLEAVFARAVELEIAFF 205
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 16-215 1.52e-19

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 86.57  E-value: 1.52e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  16 EYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYceqwgLT 95
Cdd:PRK09517 560 EINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAECIVVEAELHRSY-----LS 634

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  96 ESDLENEPEDFgTVAYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENtviEGNPYASWINLYGGEEFQSGVA 175
Cdd:PRK09517 635 GKEAPSAPSPV-TMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLAEQNH---DEHPYKDWLNTYSGEEFIAGTR 710

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 727824003 176 KGAEHFNQllAEIDINSQRGQNLIQVFKTATRMEVAFWQQ 215
Cdd:PRK09517 711 AAIARVEK--ALENAGPEQRVDAARAFLSASVHEREFFDQ 748
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 3-213 3.03e-19

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 82.01  E-value: 3.03e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   3 YQDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMrralpsvhALLDSEI 82
Cdd:cd19359    1 IDKLWNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLL--------AFLAAKI 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  83 AHHVTYCEQW-GLTESDLENEPEDFGTV------AYTRYVLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDeNTVIEG 155
Cdd:cd19359   73 KSYLDYAEDFlKTCHIKLGIPDVVDGVKpspalkAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNED-PSDKNT 151

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 727824003 156 NPYASWINLYGGEefqsgvAKGAEHFNQLLAEIDINSQRGQNL-IQVFKTATRMEVAFW 213
Cdd:cd19359  152 NFYKTWIEPNLPD------PSSAKQLEFFLNANAAWSKIDREKaNEIFRQAMQLEINFF 204
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 6-217 5.84e-19

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 81.34  E-value: 5.84e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   6 LIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKArTLDDMRRALPSVHALLDSEIAHH 85
Cdd:cd19362    4 LKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLRALLRASSKA-PLDKAIKILNSVFSGRDKGMEVH 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  86 VTYCEQWGLTESDLENEPEDFGTVAYTRYvLDAGMTGDLVDLYAALAPCSIGYAVIGKMLIEDENTViegnpYASWINLY 165
Cdd:cd19362   83 KFLYSELGITEDEIRRTGYNLVNYAYTRH-LYYYSTLGWPQFLAAWAPCMWGYSEIGKYVLNSPNEL-----YKTWASFY 156

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 727824003 166 GGEEFQSGVakgaEHFNQLLAEIDINsqrgQNLIQVFKTATRMEVAFWQQGL 217
Cdd:cd19362  157 ASKDYKKRV----EAILEALDSIEDT----EDIKNIFRNSVNFEIMFWDAAL 200
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 20-213 2.42e-16

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 76.92  E-value: 2.42e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  20 HAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKARTLDDMRRALPSVHALLDSEIAHHVTYCEQwgltesdl 99
Cdd:PTZ00347  31 LPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELKNCHHHYIDN-------- 102

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003 100 enePEDFGTVA----YTRYVLDAGMTGDLVDL--YAALAPCSIGYAVIGKMLIeDENTVIEGNPYASWINLYGGEEFQsg 173
Cdd:PTZ00347 103 ---PDAAGPEAacrkYVDFLLASGNADTLGPSvvIAAVIPCARLYAWVGQELT-NEVELTESHPFRRWLLSYSDEPIN-- 176

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 727824003 174 vaKGAEHFNQLLaEIDINSQRGQNLIQVFKTATRMEVAFW 213
Cdd:PTZ00347 177 --TSVEQLESLL-DKYIRPGEFSEVAQAYRRAMELEYDFF 213
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 4-213 4.23e-13

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 65.80  E-value: 4.23e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003   4 QDLIDACRQDWQEYTEHAFVQQLAQGTLAQPCFLHYLKQDFLFLKQYARAYALAIYKAR--TLDDMRRALPS-VHAL--L 78
Cdd:cd19357    2 SHLLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLLARAPlpSSSLNQRLLDVlLGALanL 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 727824003  79 DSEIAHHVTYCEQWGLTESDLENEPEDfGTVAYTRYVLDAGMTG-DLVDLYAALapcsigYAV----------IGKMLIE 147
Cdd:cd19357   82 RRELAFFEETAAEYGLDLPGLGVPPSP-ATRAYVDFLASLASEGvSYLEGLVVL------WATekvyldawsyARSFLPS 154

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 727824003 148 DENTvieGNPYASWINLYGGEEFQSGVAKGAEHFNQLLAEIDinSQRGQNLIQVFKTATRMEVAFW 213
Cdd:cd19357  155 DADG---GALYREFIPNWTSPEFAAFVDRLGDLVDEALEQAG--EEVLERAEEVWRRVLELEEAFW 215
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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