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Conserved domains on  [gi|730340747|ref|WP_033990916|]
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MULTISPECIES: IS91 family transposase [Gammaproteobacteria]

Protein Classification

IS91 family transposase( domain architecture ID 1750273)

IS91 family transposase is the prototype element of a family of bacterial insertion sequences that transpose by a rolling-circle mechanism.

Gene Ontology:  GO:0003677|GO:0004803|GO:0006313
PubMed:  19709290|20215432

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
transpos_IS91 super family cl41310
IS91 family transposase;
21-461 7.74e-69

IS91 family transposase;


The actual alignment was detected with superfamily member NF033538:

Pssm-ID: 468069 [Multi-domain]  Cd Length: 376  Bit Score: 224.91  E-value: 7.74e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  21 YALVEAHYPDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGfCPSCGARRMAESAR 100
Cdd:NF033538   1 LKDIFEANQHWDAFLAQHGGRLRPVQLEAVTKMLACRTSALGGHHYRCDNCGHVKYVYNSCKSRH-CPSCGAKATEQWIA 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 101 HLVEEVFgPRPVRQWVLSFPYPLRFLFASKPEaigPVLGIVQRVIAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIH 180
Cdd:NF033538  80 KQQAELL-PCPYRHVVFTLPDELWPLARHNRA---WLLGALFRSAADTLLTFAKNRRHLLEIGAFAVLHTWGRRLNWHPH 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 181 FHMLWLDGVYVEATelprrelRLHRARAPTTAQLTQLAATIAHRVCRHLTRKgWLEGEGESAFLAdsaagddsmdglrms 260
Cdd:NF033538 156 LHLLVTAGGLDEDG-------RWKKCKGGILFPAKALSKVWRAKFLTLLRQA-YDAGELFPAELA--------------- 212
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 261 sityriatgrdagckvvtLQTLPGdAGSLEGEAGKVGGFSLHAGVaAEAHESHKLEKLCRYITRPAISEKRLSIALQGRV 340
Cdd:NF033538 213 ------------------HIKLPS-AWNKFLLGLYPRGWVVHAKK-KTGNGRQVLEYLGRYLKRPAISNSRLRHYDGGEV 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 341 RYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNAALRAQltpsgrgrrhdaavepadASANDAPRS 420
Cdd:NF033538 273 TFRYKDHRTKGTKTLTLSPDEFIRRFLQHVPPKGFRRIRYYGFLANRSRGRAL------------------PTVYAALPE 334
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 730340747 421 PEEKRRS-MSWAQRLKRVFSIDVTACVHCGGTVRIVASIEEP 461
Cdd:NF033538 335 PPPKKAApLVFAQMLKRLLKVDPPECPLCGGRMVFIAFIRGP 376
 
Name Accession Description Interval E-value
transpos_IS91 NF033538
IS91 family transposase;
21-461 7.74e-69

IS91 family transposase;


Pssm-ID: 468069 [Multi-domain]  Cd Length: 376  Bit Score: 224.91  E-value: 7.74e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  21 YALVEAHYPDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGfCPSCGARRMAESAR 100
Cdd:NF033538   1 LKDIFEANQHWDAFLAQHGGRLRPVQLEAVTKMLACRTSALGGHHYRCDNCGHVKYVYNSCKSRH-CPSCGAKATEQWIA 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 101 HLVEEVFgPRPVRQWVLSFPYPLRFLFASKPEaigPVLGIVQRVIAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIH 180
Cdd:NF033538  80 KQQAELL-PCPYRHVVFTLPDELWPLARHNRA---WLLGALFRSAADTLLTFAKNRRHLLEIGAFAVLHTWGRRLNWHPH 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 181 FHMLWLDGVYVEATelprrelRLHRARAPTTAQLTQLAATIAHRVCRHLTRKgWLEGEGESAFLAdsaagddsmdglrms 260
Cdd:NF033538 156 LHLLVTAGGLDEDG-------RWKKCKGGILFPAKALSKVWRAKFLTLLRQA-YDAGELFPAELA--------------- 212
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 261 sityriatgrdagckvvtLQTLPGdAGSLEGEAGKVGGFSLHAGVaAEAHESHKLEKLCRYITRPAISEKRLSIALQGRV 340
Cdd:NF033538 213 ------------------HIKLPS-AWNKFLLGLYPRGWVVHAKK-KTGNGRQVLEYLGRYLKRPAISNSRLRHYDGGEV 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 341 RYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNAALRAQltpsgrgrrhdaavepadASANDAPRS 420
Cdd:NF033538 273 TFRYKDHRTKGTKTLTLSPDEFIRRFLQHVPPKGFRRIRYYGFLANRSRGRAL------------------PTVYAALPE 334
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 730340747 421 PEEKRRS-MSWAQRLKRVFSIDVTACVHCGGTVRIVASIEEP 461
Cdd:NF033538 335 PPPKKAApLVFAQMLKRLLKVDPPECPLCGGRMVFIAFIRGP 376
Y2_Tnp pfam04986
Putative transposase; Transposases are needed for efficient transposition of the insertion ...
163-386 8.94e-38

Putative transposase; Transposases are needed for efficient transposition of the insertion sequence or transposon DNA. This family includes transposases IS1294 and IS801. This is a rolling-circle transposase.


Pssm-ID: 428238  Cd Length: 183  Bit Score: 136.64  E-value: 8.94e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  163 GAVTLIQRFGSALNLNIHFHMLWLDGVYVE-ATELPRRELRLHRARAPTTAQLTQLAATIAHRVCRHLTRKGWLEGEGES 241
Cdd:pfam04986   1 GFIAVLHTWGRALNLHPHVHCLVPGGGLDEdGQWWASRGGFFLPVKALSRLFRGKLLETLRKAILRGKLKLGLLERPSAF 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  242 AFLADSAAGDdsmdglrmssityriatgrdagckvvtlqtlpgdagslegeagkvgGFSLHAGVAAeAHESHKLEKLCRY 321
Cdd:pfam04986  81 AYLLLKAWDK----------------------------------------------GWVVDAKVPF-GGGRAVLEYLGRY 113
                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 730340747  322 ITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAP 386
Cdd:pfam04986 114 IHRPAISNSRLASTDDGQVTFRYKDYRGGRTKTMTLPPLEFIRRFLQHVPPPGFHRIRYYGFLAN 178
 
Name Accession Description Interval E-value
transpos_IS91 NF033538
IS91 family transposase;
21-461 7.74e-69

IS91 family transposase;


Pssm-ID: 468069 [Multi-domain]  Cd Length: 376  Bit Score: 224.91  E-value: 7.74e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  21 YALVEAHYPDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGfCPSCGARRMAESAR 100
Cdd:NF033538   1 LKDIFEANQHWDAFLAQHGGRLRPVQLEAVTKMLACRTSALGGHHYRCDNCGHVKYVYNSCKSRH-CPSCGAKATEQWIA 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 101 HLVEEVFgPRPVRQWVLSFPYPLRFLFASKPEaigPVLGIVQRVIAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIH 180
Cdd:NF033538  80 KQQAELL-PCPYRHVVFTLPDELWPLARHNRA---WLLGALFRSAADTLLTFAKNRRHLLEIGAFAVLHTWGRRLNWHPH 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 181 FHMLWLDGVYVEATelprrelRLHRARAPTTAQLTQLAATIAHRVCRHLTRKgWLEGEGESAFLAdsaagddsmdglrms 260
Cdd:NF033538 156 LHLLVTAGGLDEDG-------RWKKCKGGILFPAKALSKVWRAKFLTLLRQA-YDAGELFPAELA--------------- 212
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 261 sityriatgrdagckvvtLQTLPGdAGSLEGEAGKVGGFSLHAGVaAEAHESHKLEKLCRYITRPAISEKRLSIALQGRV 340
Cdd:NF033538 213 ------------------HIKLPS-AWNKFLLGLYPRGWVVHAKK-KTGNGRQVLEYLGRYLKRPAISNSRLRHYDGGEV 272
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747 341 RYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNAALRAQltpsgrgrrhdaavepadASANDAPRS 420
Cdd:NF033538 273 TFRYKDHRTKGTKTLTLSPDEFIRRFLQHVPPKGFRRIRYYGFLANRSRGRAL------------------PTVYAALPE 334
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 730340747 421 PEEKRRS-MSWAQRLKRVFSIDVTACVHCGGTVRIVASIEEP 461
Cdd:NF033538 335 PPPKKAApLVFAQMLKRLLKVDPPECPLCGGRMVFIAFIRGP 376
Y2_Tnp pfam04986
Putative transposase; Transposases are needed for efficient transposition of the insertion ...
163-386 8.94e-38

Putative transposase; Transposases are needed for efficient transposition of the insertion sequence or transposon DNA. This family includes transposases IS1294 and IS801. This is a rolling-circle transposase.


Pssm-ID: 428238  Cd Length: 183  Bit Score: 136.64  E-value: 8.94e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  163 GAVTLIQRFGSALNLNIHFHMLWLDGVYVE-ATELPRRELRLHRARAPTTAQLTQLAATIAHRVCRHLTRKGWLEGEGES 241
Cdd:pfam04986   1 GFIAVLHTWGRALNLHPHVHCLVPGGGLDEdGQWWASRGGFFLPVKALSRLFRGKLLETLRKAILRGKLKLGLLERPSAF 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747  242 AFLADSAAGDdsmdglrmssityriatgrdagckvvtlqtlpgdagslegeagkvgGFSLHAGVAAeAHESHKLEKLCRY 321
Cdd:pfam04986  81 AYLLLKAWDK----------------------------------------------GWVVDAKVPF-GGGRAVLEYLGRY 113
                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 730340747  322 ITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAP 386
Cdd:pfam04986 114 IHRPAISNSRLASTDDGQVTFRYKDYRGGRTKTMTLPPLEFIRRFLQHVPPPGFHRIRYYGFLAN 178
Zn_Tnp_IS91 pfam14319
Transposase zinc-binding domain; This domain is likely to be a zinc-binding domain. It is ...
23-119 4.63e-23

Transposase zinc-binding domain; This domain is likely to be a zinc-binding domain. It is found at the N-terminus of transposases belonging to the IS91 family.


Pssm-ID: 464134  Cd Length: 90  Bit Score: 93.01  E-value: 4.63e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 730340747   23 LVEAHYPDFIARieaegRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRgFCPSCGARRMAESARHL 102
Cdd:pfam14319   1 IFRRHWDDYLAK-----HSLSPHQRKALEAILNCRTAALGGHVYRCEDCGHERIVYNSCRNR-HCPSCQAKATEQWLERQ 74
                          90
                  ....*....|....*..
gi 730340747  103 VEEVFgPRPVRQWVLSF 119
Cdd:pfam14319  75 QAELL-PVPYFHVVFTL 90
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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