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Conserved domains on  [gi|730624698|ref|WP_034055994|]
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23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, partial [Pseudomonas aeruginosa]

Protein Classification

RNA methyltransferase( domain architecture ID 1000317)

SpoU family RNA methyltransferase catalyzes the methylation of rRNA or tRNA at specific sites in a S-adenosyl-L-methionine (SAM)-dependent manner

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
SPOUT_MTase super family cl38912
SPOUT superfamily of SAM-dependent RNA methyltransferases; The SPOUT (SpoU-TrmD) ...
1-69 6.44e-23

SPOUT superfamily of SAM-dependent RNA methyltransferases; The SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, also known as class IV methyltransferase family, is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot. Members of the SPOUT superfamily that have been characterized functionally are involved in post-transcriptional RNA modification by catalyzing methylation of the 2-OH group of ribose, the N-1 atom of guanosine 37 in tRNA, or the N-3 atom of uridine 1498 in 16S rRNA.


The actual alignment was detected with superfamily member TIGR00246:

Pssm-ID: 476814  Cd Length: 153  Bit Score: 84.98  E-value: 6.44e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 730624698    1 ADVARLIRQEGEAMLARVQPGeRVVTLEVEGRPWSTEQLARELDRWRLDARTVNLMVGGPEGLAPEVCA 69
Cdd:TIGR00246  46 ADIKRILDKEGDRILAAIGKA-HVVTLDIPGKPWTTPQLADTLEKWKTDGRDVTLLIGGPEGLSPTCKA 113
 
Name Accession Description Interval E-value
tRNA_RlmH_YbeA TIGR00246
rRNA large subunit m3Psi methyltransferase RlmH; This protein, in the SPOUT methyltransferase ...
1-69 6.44e-23

rRNA large subunit m3Psi methyltransferase RlmH; This protein, in the SPOUT methyltransferase family, previously designated YbeA in E. coli, was shown to be responsible for a further modification, a methylation, to a pseudouridine base in ribosomal large subunit RNA. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129349  Cd Length: 153  Bit Score: 84.98  E-value: 6.44e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 730624698    1 ADVARLIRQEGEAMLARVQPGeRVVTLEVEGRPWSTEQLARELDRWRLDARTVNLMVGGPEGLAPEVCA 69
Cdd:TIGR00246  46 ADIKRILDKEGDRILAAIGKA-HVVTLDIPGKPWTTPQLADTLEKWKTDGRDVTLLIGGPEGLSPTCKA 113
 
Name Accession Description Interval E-value
tRNA_RlmH_YbeA TIGR00246
rRNA large subunit m3Psi methyltransferase RlmH; This protein, in the SPOUT methyltransferase ...
1-69 6.44e-23

rRNA large subunit m3Psi methyltransferase RlmH; This protein, in the SPOUT methyltransferase family, previously designated YbeA in E. coli, was shown to be responsible for a further modification, a methylation, to a pseudouridine base in ribosomal large subunit RNA. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129349  Cd Length: 153  Bit Score: 84.98  E-value: 6.44e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 730624698    1 ADVARLIRQEGEAMLARVQPGeRVVTLEVEGRPWSTEQLARELDRWRLDARTVNLMVGGPEGLAPEVCA 69
Cdd:TIGR00246  46 ADIKRILDKEGDRILAAIGKA-HVVTLDIPGKPWTTPQLADTLEKWKTDGRDVTLLIGGPEGLSPTCKA 113
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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