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Conserved domains on  [gi|739227149|ref|WP_037090448|]
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MULTISPECIES: four-carbon acid sugar kinase family protein [Rhizobium/Agrobacterium group]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
OtnK super family cl34601
D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family ...
1-340 1.79e-35

D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family [Carbohydrate transport and metabolism];


The actual alignment was detected with superfamily member COG3395:

Pssm-ID: 442622 [Multi-domain]  Cd Length: 415  Bit Score: 133.40  E-value: 1.79e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149   1 MLLIFADDLTGALDAAAPFAGRGMVTEVATGLEgvHAALAAAPAVLSVNLGCRDGKADEARRRTQELLALVPAD--TILF 78
Cdd:COG3395    1 KLGVIADDFTGATDVAVQLARAGLRTVLLLGVP--TLALADDADAVVIATKSRSLPPEEAVARVREALAWLKAAgaRLVY 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149  79 KKIDSRLKGHIAAEMDAI----SFRRALVVPAIPDFGRIVSGGAV---------SGF----------------------- 122
Cdd:COG3395   79 KKFDSTLRGNIGAETDALldalGADAAVVVPAFPENGRTTVGGHLfvggvplheTEMardpvtpmtesdlprllaeqtkg 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 123 ------------GVETAIAIDEKLGRHALRCSIPDTRTTDDIRQ-ALSAAQAEGVDLFIGARGLAETLASvmaarvLPDV 189
Cdd:COG3395  159 pvglvdladvraGAEALRAALAALAAEGARIVVVDAVTDADLDAiAEALADLAERVLVVGSSGLAAALAA------APAA 232
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 190 ATVPPGKGIFVIGSRDPITLEQIDVLKSADLVSIIEAPNGAVPRDEEED---------------------------VDLS 242
Cdd:COG3395  233 LPPAGGPVLVVVGSCSPVTRRQLAALLAEPGVPVVELDVERLLDGEAEAeveralawalaalaagrtvliytsrdpEDVA 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 243 LIQATQGDEYLSSLeVSDRLAE---GVVPHftAGAGRLLLSGGATAEAVLRAMGILRLRLAGECLPGLGVGWAGNQC--- 316
Cdd:COG3395  313 DAQERLGRLAAGER-IEAALAEiarRLLEE--AGVRRLIVAGGDTSGAVLKALGIRGLRILGEIAPGVPLGRAIGGDfdg 389
                        410       420
                 ....*....|....*....|....*.
gi 739227149 317 --IIAKSGGFGQADTLKRIAKMMLGA 340
Cdd:COG3395  390 lpVVLKGGNFGDEDFFARALEGLEGK 415
 
Name Accession Description Interval E-value
OtnK COG3395
D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family ...
1-340 1.79e-35

D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family [Carbohydrate transport and metabolism];


Pssm-ID: 442622 [Multi-domain]  Cd Length: 415  Bit Score: 133.40  E-value: 1.79e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149   1 MLLIFADDLTGALDAAAPFAGRGMVTEVATGLEgvHAALAAAPAVLSVNLGCRDGKADEARRRTQELLALVPAD--TILF 78
Cdd:COG3395    1 KLGVIADDFTGATDVAVQLARAGLRTVLLLGVP--TLALADDADAVVIATKSRSLPPEEAVARVREALAWLKAAgaRLVY 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149  79 KKIDSRLKGHIAAEMDAI----SFRRALVVPAIPDFGRIVSGGAV---------SGF----------------------- 122
Cdd:COG3395   79 KKFDSTLRGNIGAETDALldalGADAAVVVPAFPENGRTTVGGHLfvggvplheTEMardpvtpmtesdlprllaeqtkg 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 123 ------------GVETAIAIDEKLGRHALRCSIPDTRTTDDIRQ-ALSAAQAEGVDLFIGARGLAETLASvmaarvLPDV 189
Cdd:COG3395  159 pvglvdladvraGAEALRAALAALAAEGARIVVVDAVTDADLDAiAEALADLAERVLVVGSSGLAAALAA------APAA 232
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 190 ATVPPGKGIFVIGSRDPITLEQIDVLKSADLVSIIEAPNGAVPRDEEED---------------------------VDLS 242
Cdd:COG3395  233 LPPAGGPVLVVVGSCSPVTRRQLAALLAEPGVPVVELDVERLLDGEAEAeveralawalaalaagrtvliytsrdpEDVA 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 243 LIQATQGDEYLSSLeVSDRLAE---GVVPHftAGAGRLLLSGGATAEAVLRAMGILRLRLAGECLPGLGVGWAGNQC--- 316
Cdd:COG3395  313 DAQERLGRLAAGER-IEAALAEiarRLLEE--AGVRRLIVAGGDTSGAVLKALGIRGLRILGEIAPGVPLGRAIGGDfdg 389
                        410       420
                 ....*....|....*....|....*.
gi 739227149 317 --IIAKSGGFGQADTLKRIAKMMLGA 340
Cdd:COG3395  390 lpVVLKGGNFGDEDFFARALEGLEGK 415
SBD_N pfam07005
Sugar-binding N-terminal domain; This is the N-terminal domain found in proteins in a range of ...
2-117 9.99e-15

Sugar-binding N-terminal domain; This is the N-terminal domain found in proteins in a range of Proteobacteria as well as the Gram-positive Oceanobacillus iheyensis. Structural analysis of the whole protein indicates the N- and C-termini act together to produce a surface into which a threonate-ADP complex is bound, demonstrating that a sugar binding site is on the N-terminal domain, and a nucleotide binding site is in the C-terminal domain. There is a critical motif, DDXTG, at approximately residues 22-25. Proteins containing this domain have been predicted as kinases. Some members are associated with PdxA2 by physical clustering and gene fusion with PdxA2. Some members that are fused with PdxA2 have been shown to be involved in L-4-hydroxythreonine (4HT) phosphorylation, part of the alternative pathway to make PLP (pyridoxal 5'-phosphate) out of a toxic metabolite, 4HT. However, 4HT phosphorylation might not be the main function of this group of proteins. Moreover, some members that are not associated with pdxA2, and even one that is associated with pdxA2, have lost 4HT kinase activity. Functional analysis demonstrate that family members include D-Threonate kinases (DtnK), D-Erythronate kinases (DenK) and 3-Oxo-tetronate kinases (OtnK).


Pssm-ID: 462065  Cd Length: 229  Bit Score: 72.57  E-value: 9.99e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149    2 LLIFADDLTGALDAAAPFAGRGMvtEVATGLEGVHAALAAAPAVLSVNLGCRDGKADEARRRTQELLALVPAD--TILFK 79
Cdd:pfam07005   1 LGVIADDFTGAQDVGVQLAKHGL--RTLVFLGVPDAARLPDADAVVIATNSRSLPPEEAVARVREALKWLAALgaRLYYK 78
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 739227149   80 ---KIDSRLKGHIAAEMDAI-----SFRRALVVPAIPDFGRIVSGG 117
Cdd:pfam07005  79 vcsRFDSTLRGNIGAETDALldalgAFDAAVVAPAFPEGGRTTIGG 124
 
Name Accession Description Interval E-value
OtnK COG3395
D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family ...
1-340 1.79e-35

D-threonate/D-erythronate kinase OtnK and related C4-acid sugar kinases, YgbK/DUF1537 family [Carbohydrate transport and metabolism];


Pssm-ID: 442622 [Multi-domain]  Cd Length: 415  Bit Score: 133.40  E-value: 1.79e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149   1 MLLIFADDLTGALDAAAPFAGRGMVTEVATGLEgvHAALAAAPAVLSVNLGCRDGKADEARRRTQELLALVPAD--TILF 78
Cdd:COG3395    1 KLGVIADDFTGATDVAVQLARAGLRTVLLLGVP--TLALADDADAVVIATKSRSLPPEEAVARVREALAWLKAAgaRLVY 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149  79 KKIDSRLKGHIAAEMDAI----SFRRALVVPAIPDFGRIVSGGAV---------SGF----------------------- 122
Cdd:COG3395   79 KKFDSTLRGNIGAETDALldalGADAAVVVPAFPENGRTTVGGHLfvggvplheTEMardpvtpmtesdlprllaeqtkg 158
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 123 ------------GVETAIAIDEKLGRHALRCSIPDTRTTDDIRQ-ALSAAQAEGVDLFIGARGLAETLASvmaarvLPDV 189
Cdd:COG3395  159 pvglvdladvraGAEALRAALAALAAEGARIVVVDAVTDADLDAiAEALADLAERVLVVGSSGLAAALAA------APAA 232
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 190 ATVPPGKGIFVIGSRDPITLEQIDVLKSADLVSIIEAPNGAVPRDEEED---------------------------VDLS 242
Cdd:COG3395  233 LPPAGGPVLVVVGSCSPVTRRQLAALLAEPGVPVVELDVERLLDGEAEAeveralawalaalaagrtvliytsrdpEDVA 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149 243 LIQATQGDEYLSSLeVSDRLAE---GVVPHftAGAGRLLLSGGATAEAVLRAMGILRLRLAGECLPGLGVGWAGNQC--- 316
Cdd:COG3395  313 DAQERLGRLAAGER-IEAALAEiarRLLEE--AGVRRLIVAGGDTSGAVLKALGIRGLRILGEIAPGVPLGRAIGGDfdg 389
                        410       420
                 ....*....|....*....|....*.
gi 739227149 317 --IIAKSGGFGQADTLKRIAKMMLGA 340
Cdd:COG3395  390 lpVVLKGGNFGDEDFFARALEGLEGK 415
SBD_N pfam07005
Sugar-binding N-terminal domain; This is the N-terminal domain found in proteins in a range of ...
2-117 9.99e-15

Sugar-binding N-terminal domain; This is the N-terminal domain found in proteins in a range of Proteobacteria as well as the Gram-positive Oceanobacillus iheyensis. Structural analysis of the whole protein indicates the N- and C-termini act together to produce a surface into which a threonate-ADP complex is bound, demonstrating that a sugar binding site is on the N-terminal domain, and a nucleotide binding site is in the C-terminal domain. There is a critical motif, DDXTG, at approximately residues 22-25. Proteins containing this domain have been predicted as kinases. Some members are associated with PdxA2 by physical clustering and gene fusion with PdxA2. Some members that are fused with PdxA2 have been shown to be involved in L-4-hydroxythreonine (4HT) phosphorylation, part of the alternative pathway to make PLP (pyridoxal 5'-phosphate) out of a toxic metabolite, 4HT. However, 4HT phosphorylation might not be the main function of this group of proteins. Moreover, some members that are not associated with pdxA2, and even one that is associated with pdxA2, have lost 4HT kinase activity. Functional analysis demonstrate that family members include D-Threonate kinases (DtnK), D-Erythronate kinases (DenK) and 3-Oxo-tetronate kinases (OtnK).


Pssm-ID: 462065  Cd Length: 229  Bit Score: 72.57  E-value: 9.99e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149    2 LLIFADDLTGALDAAAPFAGRGMvtEVATGLEGVHAALAAAPAVLSVNLGCRDGKADEARRRTQELLALVPAD--TILFK 79
Cdd:pfam07005   1 LGVIADDFTGAQDVGVQLAKHGL--RTLVFLGVPDAARLPDADAVVIATNSRSLPPEEAVARVREALKWLAALgaRLYYK 78
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 739227149   80 ---KIDSRLKGHIAAEMDAI-----SFRRALVVPAIPDFGRIVSGG 117
Cdd:pfam07005  79 vcsRFDSTLRGNIGAETDALldalgAFDAAVVAPAFPEGGRTTIGG 124
NBD_C pfam17042
Nucleotide-binding C-terminal domain; This is the C-terminal domain found in proteins in a ...
200-330 5.40e-09

Nucleotide-binding C-terminal domain; This is the C-terminal domain found in proteins in a range of Proteobacteria as well as the Gram-positive Oceanobacillus iheyensis. Structural analysis of the whole protein indicates the N- and C-termini act together to produce a surface into which a threonate-ADP complex is bound, demonstrating that a sugar binding site is on the N-terminal domain, and a nucleotide binding site is in the C-terminal domain. There is a critical motif, DDXTG, at approximately residues 22-25. Proteins containing this domain have been predicted as kinases. Some members are associated with PdxA2 by physical clustering and gene fusion with PdxA2. Some members that are fused with PdxA2 have been shown to be involved in L-4-hydroxythreonine (4HT) phosphorylation, part of the alternative pathway to make PLP (pyridoxal 5'-phosphate) out of a toxic metabolite, 4HT. However, 4HT phosphorylation might not be the main function of this group of proteins. Moreover, some members that are not associated with pdxA2, and even one that is associated with pdxA2, have lost 4HT kinase activity. Functional analysis demonstrate that family members include D-Threonate kinases (DtnK), D-Erythronate kinases (DenK) and 3-Oxo-tetronate kinases (OtnK).


Pssm-ID: 465337  Cd Length: 166  Bit Score: 54.50  E-value: 5.40e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149  200 VIGSRDPITLEQIDVLKSADLVSIIE--------------------------APNGAVP-----RDEEEDVDLSLIQATQ 248
Cdd:pfam17042   3 VVGSCSPKTTAQLAALLAERGVVVVEldvealldeeareeeieralaealaaLASGKDVvvytsRGPEDVAALDSLQAAL 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 739227149  249 GDEYLSSLeVSDRLAEGVVPHFTAGAGRLLLSGGATAEAVLRAMGILRLRLAGECLPG--LGVGWAGNQC-IIAKSGGFG 325
Cdd:pfam17042  83 GLSRAGAR-ISAALAEIARGLLARGVRGLVVAGGDTSGAVLKALGIRGLRVLGEIAPGvpLGRLIGAPGLpVVLKGGNFG 161

                  ....*
gi 739227149  326 QADTL 330
Cdd:pfam17042 162 DEDAL 166
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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