Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; Molybdopterin synthase sulfur carrier subunit MoaD is part of the Pathway/BioSystem: Molybdopterin biosynthesis

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746011283  1 MVEVRFFGPIKE------ENFFVKAG-DLKELRAILQEK-EGLKEWL--GVCAIALNDHLIDnLDTPLKDGDVVSLLPPV 70
Cdd:COG1977   1 KVTVRYFAALREaagkseEEVELPEGaTVGDLLEALAARyPGLAERLlrRRVRVAVNGEDVR-LDTPLKDGDEVALFPPV 79

                ...
gi 746011283 71 CGG 73
Cdd:COG1977  80 AGG 82

Molybdopterin synthase sulfur carrier subunit

                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*....
gi 746011283 25 LRAILQEKEGLKEWLGVCAIALNDHLIDNlDTPLKDGDVVSLLPPVCGG 73
Cdd:PLN02799 35 LAELVAKFPSLEEVRSCCVLALNEEYTTE-SAALKDGDELAIIPPISGG 82

Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; Molybdopterin synthase sulfur carrier subunit MoaD is part of the Pathway/BioSystem: Molybdopterin biosynthesis

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746011283  1 MVEVRFFGPIKE------ENFFVKAG-DLKELRAILQEK-EGLKEWL--GVCAIALNDHLIDnLDTPLKDGDVVSLLPPV 70
Cdd:COG1977   1 KVTVRYFAALREaagkseEEVELPEGaTVGDLLEALAARyPGLAERLlrRRVRVAVNGEDVR-LDTPLKDGDEVALFPPV 79

                ...
gi 746011283 71 CGG 73
Cdd:COG1977  80 AGG 82

ubiquitin-like (Ubl) domain found in a group of small sulfide carrier proteins; Ubiquitin-like (Ubl) domain found in a group of small sulfide carrier proteins This family includes ThiS, MoaD, CysO, QbsE, and their homologs, which are structurally homologous to ubiquitin (Ub) and may function as the sulfide donor for the biosynthesis of thiamin, molybdopterin, cysteine, thioquinolobactin, and other sulfur-containing natural products. Ub is a protein modifier in eukaryotes that is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. Ubiquitination is comprised of a cascade of E1, E2 and E3 enzymes that results in a covalent bond between the C-terminus of Ub and the epsilon-amino group of a substrate lysine. Like Ub, small sulfide carrier proteins in this family are adenylated at a diglycyl C-terminus by specific activating proteins. The adenylated C-terminus is subsequently converted to a thiocarboxylate, serving as the sulfide source. Those activating proteins are diverse and show little sequence similarity. This family also includes the small archaeal modifier protein (SAMP), including SAMP1, SAMP2 and SAMP3, which are Ub-like proteins that function as protein modifiers and are required for the production of sulfur-containing biomolecules in the archaeon Haloferax volcanii. SAMP1 and SAMP2 are involved in sulfur transfer during molybdenum cofactor biosynthesis and tRNA thiolation much like MoaD and Urm1, respectively. They can form covalent conjugates with their protein targets through an isopeptide linkage via their C-terminal diglycine motif in a streamlined archaeal E1-dependent pathway. SAMP2 also forms homo-conjugates through the intermolecular isopeptide bond between the C-terminal Gly and the Lys58 side chain, a feature that likely resembles polyubiquitination. SAMP3 conjugates are dependent on the Ub-activating E1 enzyme homolog of archaea (UbaA) for synthesis and are cleaved by the JAMM/MPN+ domain metalloprotease HvJAMM1.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746011283  2 VEVRFFGPIK-----EENFFVKAG-DLKE-LRAILQEKEGLKEWLG-------VCAIALNDHLI--DNLDTPLKDGDVVS 65
Cdd:cd17040   1 VKVRLFGALReagagEEEIEVEGGtTVRDlLDALSERYPGLFEALDedgelrpFILVFVNGRDVrlDDGLTPLKDGDEVD 80

                ....*...
gi 746011283 66 LLPPVCGG 73
Cdd:cd17040  81 ILPPVAGG 88

MoaD family protein, archaeal; Members of this family appear to be archaeal versions of MoaD, subunit 1 of molybdopterin converting factor. This model has been split from the bacterial/eukaryotic equivalog model TIGR01682 because the presence of two members of this family in a substantial number of archaeal species suggests that roles might not be interchangeable. [Biosynthesis of cofactors, prosthetic groups, and carriers, Molybdopterin]

                         10        20        30
                 ....*....|....*....|....*....|.
gi 746011283  44 IALNDHLIDNLD-TPLKDGDVVSLLPPVCGG 73
Cdd:TIGR01687 58 ILVNGRNVDWGLgTELKDGDVVAIFPPVSGG 88

ubiquitin-like (Ubl) domain found in Mycobacterium tuberculosis CysO and similar proteins; CysO, also termed 9.5 kDa culture filtrate antigen cfp10A, together with CysM (Cysteine synthase M), forms a protein complex CysM-CysO that represents a new cysteine biosynthetic pathway in Mycobacterium tuberculosis. The replacement of the acetyl group of O-acetylserine by CysO thiocarboxylate to generate a protein-bound cysteine is catalyzed by CysM in a pyridoxal 5?-phosphate (PLP)-dependent manner. The family also includes QbsE that functions as the sulfide donor for the biosynthesis of thioquinolobactin in Pseudomonas fluorescens. A JAMM motif protein QbsD catalyzes removal of the carboxy-terminal dipeptide from QbsE. Both CysO and QbsE are similar to prokaryotic sulfur carrier proteins such as ThiS and MoaD, containing the beta-grasp ubiquitin-like fold.

                        10        20        30
                ....*....|....*....|....*....|...
gi 746011283 44 IALNDHLI---DNLDTPLKDGDVVSLLPPVCGG 73
Cdd:cd17074  57 IYVNDEDIrflQGLDTALKDGDEISIVPAIAGG 89

ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine biosynthesis protein (ThiaminS), is a sulfur carrier protein involved in thiamin biosynthesis in prokaryotes. It has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub), and is activated in an ATP-dependent manner by sulfurtransferases, similar to the activation mechanism of Ub-activating enzyme E1. ThiS has common evolutionary origin with Ub-related protein modifiers in eukaryotes, a beta-grasp fold as Ub, and is closely related to proteins MoaD and Urm1.

                        10        20        30
                ....*....|....*....|....*....|....*
gi 746011283 42 CAIALNDHLI---DNLDTPLKDGDVVSLLPPVCGG 73
Cdd:cd00565  30 VAVELNGEIVprsEWAETILKDGDRIEIVTFVGGG 64

thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like thiamine biosynthesis protein related to MoaD, a molybdenum cofactor biosynthesis protein. Both proteins are involved in sulfur transfer. ThiS has a conserved Gly-Gly C-terminus that is modified, in reactions requiring ThiI, ThiF, IscS, and a sulfur atom from Cys, into the thiocarboxylate that provides the sulfur for thiazole biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Thiamine]

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 746011283  27 AILQEKEGLKEwlGVCAIALNDHLIDNL---DTPLKDGDVVSLLPPVCGG 73
Cdd:TIGR01683 17 AALLESLGLDP--RRVAVAVNGEIVPRSewdDTILKEGDRIEIVTFVGGG 64