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Conserved domains on  [gi|748697987|ref|WP_039956108|]
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MULTISPECIES: heteromeric transposase endonuclease subunit TnsA [Xanthomonas]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TnsA_like_Actin super family cl40323
TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 ...
 6-194 1.14e-23

TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 contains multiple subunit. Members of this family are largely restricted to the Actinobacteria, resemble the endonuclease subunit TsnA of the multimeric transposase of Tn7 and its relatives, and occur in genomic neighborhoods that suggest a similar role in transposition.


The actual alignment was detected with superfamily member NF033179:

Pssm-ID: 380186 [Multi-domain]  Cd Length: 212  Bit Score: 93.36  E-value: 1.14e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987   6 GGVAAFESSLERDWLLALDFDPRVQAIQVQPFSLHYEHRGTTRRYTPDVRAeyRLESGMATVVyEVKPSEELrlnwsAYR 85
Cdd:NF033179  37 GRHVGYESWLERDRLMLLDFDPDVVGIASQPFWLLWRDGGRVRRHVPDYFA--RLADGTAVVV-DVRPADRI-----GPR 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  86 PRFKAAV--RYCRDHGWRFKLVTErhIRTALLDNARFLRRYRalpeQDLYV-QQLLYTLRALgTTTPQALLAAAYWAEEs 162
Cdd:NF033179 109 DAEAFAWtrAACEAVGWRYRVVGP--PDPVLLANVRWLAGYR----HPRCAgPGLAAALREA-FAEPRPLMEGARAVGD- 180

                        170       180       190
                 ....*....|....*....|....*....|..
gi 748697987 163 RMAALPMLWKLIATGRLGADLHVPLTMSAPIW 194
Cdd:NF033179 181 PLAVLPVLFHLLWRGELTADLSRPLSERTLVR 212
 
Name Accession Description Interval E-value
TnsA_like_Actin NF033179
TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 ...
 6-194 1.14e-23

TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 contains multiple subunit. Members of this family are largely restricted to the Actinobacteria, resemble the endonuclease subunit TsnA of the multimeric transposase of Tn7 and its relatives, and occur in genomic neighborhoods that suggest a similar role in transposition.


Pssm-ID: 380186 [Multi-domain]  Cd Length: 212  Bit Score: 93.36  E-value: 1.14e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987   6 GGVAAFESSLERDWLLALDFDPRVQAIQVQPFSLHYEHRGTTRRYTPDVRAeyRLESGMATVVyEVKPSEELrlnwsAYR 85
Cdd:NF033179  37 GRHVGYESWLERDRLMLLDFDPDVVGIASQPFWLLWRDGGRVRRHVPDYFA--RLADGTAVVV-DVRPADRI-----GPR 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  86 PRFKAAV--RYCRDHGWRFKLVTErhIRTALLDNARFLRRYRalpeQDLYV-QQLLYTLRALgTTTPQALLAAAYWAEEs 162
Cdd:NF033179 109 DAEAFAWtrAACEAVGWRYRVVGP--PDPVLLANVRWLAGYR----HPRCAgPGLAAALREA-FAEPRPLMEGARAVGD- 180

                        170       180       190
                 ....*....|....*....|....*....|..
gi 748697987 163 RMAALPMLWKLIATGRLGADLHVPLTMSAPIW 194
Cdd:NF033179 181 PLAVLPVLFHLLWRGELTADLSRPLSERTLVR 212
Tn7_TnsA-like_N pfam08722
TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. ...
 27-107 1.22e-20

TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The N terminal domain of TnsA is catalytic. This domain is also found in Bacteriophage T4 gp4-like head completion protein whose nuclease activity is required for packaging. This domain contains the catalytic triad of one basic and two acidic residues that form a restriction endonuclease-like active site and conserved between TnsA with gp4 proteins.


Pssm-ID: 462579 [Multi-domain]  Cd Length: 83  Bit Score: 81.50  E-value: 1.22e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987   27 PRVQAIQVQPFSLHYEHR--GTTRRYTPDVRAEYRLESGMATVVYEVKPSEELRLNWSAYRPRFKAAVRYCRDHGWRFKL 104
Cdd:pfam08722   1 PDVIDIREQPLTIPYASElgIKTRVYTPDFLVTYKDGDGKPYEAIEVKPSKDLEDWLLRTLEKLEAAREYWEERGIPFRI 80


                  ...
gi 748697987  105 VTE 107
Cdd:pfam08722  81 ITE 83
TnsA_endonuclease-like cd22362
Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic ...
 13-193 4.74e-13

Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic element working together with TnsB, TnsC, and TnsD to facilitate insertion of the transposon. TnsA catalyzes cleavage at the transposon 5' ends, and TnsC is the activator of the composite TnsAB transposase. TnsA belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411766 [Multi-domain]  Cd Length: 234  Bit Score: 65.43  E-value: 4.74e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  13 SSLERDWLLALDFDPRVQAIQVQ-P-----------FSLHYEHRGTTRRYTPDV-----RAEYRLESGMATVVYEVKPSE 75
Cdd:cd22362   33 SDLERAAFLLLEWDPNVIDIREQyPldplegtleiaKELGIKHPRYVGTGEPYVmttdfLVTLRDPGKGRLVAISVKPSD 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  76 ELrlNWSAYRPRFKAAVRYCRDHGWRFKLVTERHIRTALLDNARFLRRYRALPEQDL-----YVQQLLYTLRALGTTTPQ 150
Cdd:cd22362  113 DL--KDPRTLEKLEIERRYWERRGIPWRLITESELPKVLARNLEWLRGARRDRLEDSseledFLDAFLRALRRNKDLPLS 190

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
gi 748697987 151 ALLAAAYWAEE-SRMAALPMLWKLIATGRLGADLHVPLTMSAPI 193
Cdd:cd22362  191 DLLERLDKALGlDLGTALRAFRHLIWTGRLDVDLSQDIELEKPL 234
4 PHA02552
head completion protein; Provisional
 11-114 6.13e-09

head completion protein; Provisional


Pssm-ID: 222870  Cd Length: 151  Bit Score: 52.74  E-value: 6.13e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  11 FESSLERDWLLALDFDPRVQA-----IQVQPFSlhyEHRGTTRRYTPDVRAEYRlesGMATVVYEVKPSEELR------- 78
Cdd:PHA02552  24 YRSSWERWFMKWLDKNPSVIKwgseeVVIPYFS---NADGKRRRYFMDFYVKVD---NGQKFLIEVKPKKETQppkkpak 97

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 748697987  79 -------------LNWSAYRPRFKAAVRYCRDHGWRFKLVTERHIRTAL 114
Cdd:PHA02552  98 mttaakkrfinevYTWSVNTDKWKAARALCEKKGWKFKIITEDELKKVF 146
 
Name Accession Description Interval E-value
TnsA_like_Actin NF033179
TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 ...
 6-194 1.14e-23

TnsA-like heteromeric transposase endonuclease subunit; The transposase of transposon Tn7 contains multiple subunit. Members of this family are largely restricted to the Actinobacteria, resemble the endonuclease subunit TsnA of the multimeric transposase of Tn7 and its relatives, and occur in genomic neighborhoods that suggest a similar role in transposition.


Pssm-ID: 380186 [Multi-domain]  Cd Length: 212  Bit Score: 93.36  E-value: 1.14e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987   6 GGVAAFESSLERDWLLALDFDPRVQAIQVQPFSLHYEHRGTTRRYTPDVRAeyRLESGMATVVyEVKPSEELrlnwsAYR 85
Cdd:NF033179  37 GRHVGYESWLERDRLMLLDFDPDVVGIASQPFWLLWRDGGRVRRHVPDYFA--RLADGTAVVV-DVRPADRI-----GPR 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  86 PRFKAAV--RYCRDHGWRFKLVTErhIRTALLDNARFLRRYRalpeQDLYV-QQLLYTLRALgTTTPQALLAAAYWAEEs 162
Cdd:NF033179 109 DAEAFAWtrAACEAVGWRYRVVGP--PDPVLLANVRWLAGYR----HPRCAgPGLAAALREA-FAEPRPLMEGARAVGD- 180

                        170       180       190
                 ....*....|....*....|....*....|..
gi 748697987 163 RMAALPMLWKLIATGRLGADLHVPLTMSAPIW 194
Cdd:NF033179 181 PLAVLPVLFHLLWRGELTADLSRPLSERTLVR 212
Tn7_TnsA-like_N pfam08722
TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. ...
 27-107 1.22e-20

TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The N terminal domain of TnsA is catalytic. This domain is also found in Bacteriophage T4 gp4-like head completion protein whose nuclease activity is required for packaging. This domain contains the catalytic triad of one basic and two acidic residues that form a restriction endonuclease-like active site and conserved between TnsA with gp4 proteins.


Pssm-ID: 462579 [Multi-domain]  Cd Length: 83  Bit Score: 81.50  E-value: 1.22e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987   27 PRVQAIQVQPFSLHYEHR--GTTRRYTPDVRAEYRLESGMATVVYEVKPSEELRLNWSAYRPRFKAAVRYCRDHGWRFKL 104
Cdd:pfam08722   1 PDVIDIREQPLTIPYASElgIKTRVYTPDFLVTYKDGDGKPYEAIEVKPSKDLEDWLLRTLEKLEAAREYWEERGIPFRI 80


                  ...
gi 748697987  105 VTE 107
Cdd:pfam08722  81 ITE 83
TnsA_endonuclease-like cd22362
Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic ...
 13-193 4.74e-13

Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic element working together with TnsB, TnsC, and TnsD to facilitate insertion of the transposon. TnsA catalyzes cleavage at the transposon 5' ends, and TnsC is the activator of the composite TnsAB transposase. TnsA belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411766 [Multi-domain]  Cd Length: 234  Bit Score: 65.43  E-value: 4.74e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  13 SSLERDWLLALDFDPRVQAIQVQ-P-----------FSLHYEHRGTTRRYTPDV-----RAEYRLESGMATVVYEVKPSE 75
Cdd:cd22362   33 SDLERAAFLLLEWDPNVIDIREQyPldplegtleiaKELGIKHPRYVGTGEPYVmttdfLVTLRDPGKGRLVAISVKPSD 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  76 ELrlNWSAYRPRFKAAVRYCRDHGWRFKLVTERHIRTALLDNARFLRRYRALPEQDL-----YVQQLLYTLRALGTTTPQ 150
Cdd:cd22362  113 DL--KDPRTLEKLEIERRYWERRGIPWRLITESELPKVLARNLEWLRGARRDRLEDSseledFLDAFLRALRRNKDLPLS 190

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
gi 748697987 151 ALLAAAYWAEE-SRMAALPMLWKLIATGRLGADLHVPLTMSAPI 193
Cdd:cd22362  191 DLLERLDKALGlDLGTALRAFRHLIWTGRLDVDLSQDIELEKPL 234
4 PHA02552
head completion protein; Provisional
 11-114 6.13e-09

head completion protein; Provisional


Pssm-ID: 222870  Cd Length: 151  Bit Score: 52.74  E-value: 6.13e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  11 FESSLERDWLLALDFDPRVQA-----IQVQPFSlhyEHRGTTRRYTPDVRAEYRlesGMATVVYEVKPSEELR------- 78
Cdd:PHA02552  24 YRSSWERWFMKWLDKNPSVIKwgseeVVIPYFS---NADGKRRRYFMDFYVKVD---NGQKFLIEVKPKKETQppkkpak 97

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 748697987  79 -------------LNWSAYRPRFKAAVRYCRDHGWRFKLVTERHIRTAL 114
Cdd:PHA02552  98 mttaakkrfinevYTWSVNTDKWKAARALCEKKGWKFKIITEDELKKVF 146
Tn7_Tnp_TnsA_C pfam08721
TnsA endonuclease C terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA ...
 110-183 1.91e-05

TnsA endonuclease C terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The C terminal domain of TnsA binds DNA.


Pssm-ID: 462578  Cd Length: 83  Bit Score: 41.51  E-value: 1.91e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 748697987  110 IRTALLDNARFLRRYRALPEQDL-------YVQQLLYTLRALGTTTPQALLAAAywAEESRMA---ALPMLWKLIATGRL 179
Cdd:pfam08721   2 IPKVLAKNIEWLHPYYRIEDSDLseeeleeLAELLLERLRENPDTTLRSLLKEL--DAEYSLEpgtSLSLIRHLVATKEI 79


                  ....
gi 748697987  180 GADL 183
Cdd:pfam08721  80 LVDL 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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