|
Name |
Accession |
Description |
Interval |
E-value |
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
1-562 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 1262.04 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 1 MQADFWNDKRPAGVPSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAV 80
Cdd:PRK12492 1 MQPDFWNDKRPAGVPSTIDLAAYKSVVEVFERSCKKFADRPAFSNLGVTLSYAELERHSAAFAAYLQQHTDLVPGDRIAV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 81 QMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMA 160
Cdd:PRK12492 81 QMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVYLNMFGKLVQEVLPDTGIEYLIEAKMGDLLPAA 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 161 KGWLINTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVL 240
Cdd:PRK12492 161 KGWLVNTVVDKVKKMVPAYHLPQAVPFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVR 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 241 ACFSQHGPDGQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVA 320
Cdd:PRK12492 241 ACLSQLGPDGQPLMKEGQEVMIAPLPLYHIYAFTANCMCMMVSGNHNVLITNPRDIPGFIKELGKWRFSALLGLNTLFVA 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 321 LMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVI 400
Cdd:PRK12492 321 LMDHPGFKDLDFSALKLTNSGGTALVKATAERWEQLTGCTIVEGYGLTETSPVASTNPYGELARLGTVGIPVPGTALKVI 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 401 DDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PRK12492 401 DDDGNELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIE 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 481 DVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK12492 481 DVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRD 560
|
..
gi 820875961 561 IA 562
Cdd:PRK12492 561 IA 562
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
1-560 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 923.39 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 1 MQADFWNDKRPAGVPSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAV 80
Cdd:PRK05677 1 MIENFWKDKYPAGIAAEINPDEYPNIQAVLKQSCQRFADKPAFSNLGKTLTYGELYKLSGAFAAWLQQHTDLKPGDRIAV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 81 QMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMA 160
Cdd:PRK05677 81 QLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLANMAHLAEKVLPKTGVKHVIVTEVADMLPPL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 161 KGWLINTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVL 240
Cdd:PRK05677 161 KRLLINAVVKHVKKMVPAYHLPQAVKFNDALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCR 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 241 ACFSQhgpdgqkLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVA 320
Cdd:PRK05677 241 ALMGS-------NLNEGCEILIAPLPLYHIYAFTFHCMAMMLIGNHNILISNPRDLPAMVKELGKWKFSGFVGLNTLFVA 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 321 LMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAFKVI 400
Cdd:PRK05677 314 LCNNEAFRKLDFSALKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETSPVVSVNPSQAI-QVGTIGIPVPSTLCKVI 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 401 DDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PRK05677 393 DDDGNELPLGEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELE 472
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 481 DVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK05677 473 DVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGeTLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
.
gi 820875961 560 D 560
Cdd:PRK05677 553 D 553
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
6-560 |
0e+00 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 806.20 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKRPAGVPSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNV 85
Cdd:PRK08974 5 WLNRYPADVPAEINPDRYQSLVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGLGLKKGDRVALMMPNL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMAKGWLI 165
Cdd:PRK08974 85 LQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILTRMGDQLSTAKGTLV 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 NTVVDKVKKMVPAYHLPQAISFKQALREGAALSH-KPvTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFS 244
Cdd:PRK08974 165 NFVVKYIKRLVPKYHLPDAISFRSALHKGRRMQYvKP-ELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQAKAAYG 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 245 qhgpdgqKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDH 324
Cdd:PRK08974 244 -------PLLHPGKELVVTALPLYHIFALTVNCLLFIELGGQNLLITNPRDIPGFVKELKKYPFTAITGVNTLFNALLNN 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 325 PGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEG 404
Cdd:PRK08974 317 EEFQELDFSSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSVNPYDLDYYSGSIGLPVPSTEIKLVDDDG 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 405 NELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIM 484
Cdd:PRK08974 397 NEVPPGEPGELWVKGPQVMLGYWQRPEATDEVI-KDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVM 475
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 485 GHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK08974 476 LHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELRD 551
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
6-560 |
0e+00 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 806.17 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKRPAGVPSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNV 85
Cdd:PRK07059 5 WLKSYPPGVPAEIDASQYPSLADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQS-RGLAKGARVAIMMPNV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMaKGWLI 165
Cdd:PRK07059 84 LQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDLLGF-KGHIV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 NTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsQ 245
Cdd:PRK07059 163 NFVVRRVKKMVPAWSLPGHVRFNDALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQMEA---W 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 246 HGPDGQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHP 325
Cdd:PRK07059 240 LQPAFEKKPRPDQLNFVCALPLYHIFALTVCGLLGMRTGGRNILIPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNNP 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 326 GFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGN 405
Cdd:PRK07059 320 DFDKLDFSKLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETSPVATCNPVDATEFSGTIGLPLPSTEVSIRDDDGN 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMG 485
Cdd:PRK07059 400 DLPLGEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVAS 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 486 HPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07059 480 HPGVLEVAAVGVPDEHSGEAVKLFVVKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELRD 554
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
2-562 |
0e+00 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 702.02 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 2 QADFWNDKRPAGVPSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQ 81
Cdd:PRK08751 3 QARPWLQSYPAGVAAEIDLEQFRTVAEVFATSVAKFADRPAYHSFGKTITYREADQLVEQFAAYLLGELQLKKGDRVALM 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 82 MPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMAK 161
Cdd:PRK08751 83 MPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPVKQVITTGLGDMLGFPK 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 162 GWLINTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLA 241
Cdd:PRK08751 163 AALVNFVVKYVKKLVPEYRINGAIRFREALALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQ 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 242 CFSQHGPdgqklIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVAL 321
Cdd:PRK08751 243 WLAGTGK-----LEEGCEVVITALPLYHIFALTANGLVFMKIGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGL 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 322 MDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVID 401
Cdd:PRK08751 318 LNTPGFDQIDFSSLKMTLGGGMAVQRSVAERWKQVTGLTLVEAYGLTETSPAACINPLTLKEYNGSIGLPIPSTDACIKD 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 402 DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIED 481
Cdd:PRK08751 398 DAGTVLAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIED 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 482 VIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK08751 478 VIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDA 557
|
.
gi 820875961 562 A 562
Cdd:PRK08751 558 A 558
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
26-559 |
0e+00 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 675.82 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 26 VVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNT 105
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQN-LGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 NPLYTEREMRHQFKDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpQAI 185
Cdd:cd05936 80 NPLYTPRELEHILNDSGAKALI-------------------------------------------------------VAV 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 186 SFKQALREGAALShKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsqhgpdgqKLIKEGQEVMIAPL 265
Cdd:cd05936 105 SFTDLLAAGAPLG-ERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWL--------EDLLEGDDVVLAAL 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 266 PLYHIYAFTANCMCMMVTGNHNVLITNPRDIpGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTAL 345
Cdd:cd05936 176 PLFHVFGLTVALLLPLALGATIVLIPRFRPI-GVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGAPL 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKG 425
Cdd:cd05936 255 PVEVAERFEELTGVPIVEGYGLTETSPVVAVNPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKG 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 426 YWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEA 505
Cdd:cd05936 335 YWNRPEETAEAFV-DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEA 413
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 506 VKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05936 414 VKAFVVLKEGAsLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
26-562 |
0e+00 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 552.11 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 26 VVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNT 105
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRAL-GVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 NPLYTEREMRHQFKDAGARALVYlnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqai 185
Cdd:COG0318 80 NPRLTAEELAYILEDSGARALVT--------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 186 sfkqalregaalshkpvtlslddiAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPL 265
Cdd:COG0318 103 ------------------------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGD----------VVLVAL 148
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 266 PLYHIYAFTANCMCMMVTGNHNVLITNpRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTAL 345
Cdd:COG0318 149 PLFHVFGLTVGLLAPLLAGATLVLLPR-FDPERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVSGGAPL 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWESLTGCRIVEGYGLTETSPVASTNPY-GELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMK 424
Cdd:COG0318 228 PPELLERFEERFGVRIVEGYGLTETSPVVTVNPEdPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVVRGPNVMK 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 425 GYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGE 504
Cdd:COG0318 308 GYWNDPEATAEAF-RDGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGE 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 820875961 505 AVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:COG0318 387 RVVAFVVLRPGAeLDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERY 445
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
4-560 |
1.83e-166 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 484.89 E-value: 1.83e-166
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 4 DFWNDKR-----PAGVPSTIDIHAyTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRI 78
Cdd:PRK05605 8 SAFADKPwlqsyAPWTPHDLDYGD-TTLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRA-LGVRPGDRV 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 79 AVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLP 158
Cdd:PRK05605 86 AIVLPNCPQHIVAFYAVLRLGAVVVEHNPLYTAHELEHPFEDHGARVAIVWDKVAPTVERLRRTTPLETIVSVNMIAAMP 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 159 MAKGWLINTVVDKVKKMVPAYH--LPQAISFKQALR-----EGAALSHKPVTLslDDIAVLQYTGGTTGLAKGAMLTHGN 231
Cdd:PRK05605 166 LLQRLALRLPIPALRKARAALTgpAPGTVPWETLVDaaiggDGSDVSHPRPTP--DDVALILYTSGTTGKPKGAQLTHRN 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 232 LVANMLQvlacfSQHGPDGqklIKEGQEVMIAPLPLYHIYAFTancMCM---MVTGNHNVLITNPrDIPGFIKELGKWRF 308
Cdd:PRK05605 244 LFANAAQ-----GKAWVPG---LGDGPERVLAALPMFHAYGLT---LCLtlaVSIGGELVLLPAP-DIDLILDAMKKHPP 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 309 TALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTV 388
Cdd:PRK05605 312 TWLPGVPPLYEKIAEAAEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSPIIVGNPMSDDRRPGYV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 389 GIPVADTAFKVID--DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDM 466
Cdd:PRK05605 392 GVPFPDTEVRIVDpeDPDETMPDGEEGELLVRGPQVFKGYWNRPEETAKSF-LDGWFRTGDVVVMEEDGFIRIVDRIKEL 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 467 IIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESL 545
Cdd:PRK05605 471 IITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAaLDPEGLRAYCREHLTRYKVPRRFYHVDEL 550
|
570
....*....|....*
gi 820875961 546 PMTPVGKILRRELRD 560
Cdd:PRK05605 551 PRDQLGKVRRREVRE 565
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
25-560 |
1.98e-138 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 411.22 E-value: 1.98e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVN 104
Cdd:PRK07656 6 TLPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAAL-GIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 105 TNPLYTEREMRHQFKDAGARALVYLNMFgkrvqevlpdTGIEYlieaKMGDLLPmakgwLINTVVdKVKKMVPAYHLPQA 184
Cdd:PRK07656 85 LNTRYTADEAAYILARGDAKALFVLGLF----------LGVDY----SATTRLP-----ALEHVV-ICETEEDDPHTEKM 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 185 ISFKQALREGAAlSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSqhgpdgqklIKEGQEVMIAp 264
Cdd:PRK07656 145 KTFTDFLAAGDP-AERAPEVDPDDVADILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLG---------LTEGDRYLAA- 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 265 LPLYHIYAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTA 344
Cdd:PRK07656 214 NPFFHVFGYKAGVNAPLMRGA-TILPLPVFDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTGAAS 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 345 LVKATAERWESLTGCRIV-EGYGLTETSPVASTNPYGELARL--GTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQ 421
Cdd:PRK07656 293 MPVALLERFESELGVDIVlTGYGLSEASGVTTFNRLDDDRKTvaGTIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPN 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 422 VMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDER 501
Cdd:PRK07656 373 VMKGYYDDPEATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDER 452
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 502 SGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07656 453 LGEVGKAYVVLKPGaELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
30-470 |
2.74e-134 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 397.07 E-value: 2.74e-134
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPAFSN-LGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPL 108
Cdd:pfam00501 1 LERQAARTPDKTALEVgEGRRLTYRELDERANRLAAGLRAL-GVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 109 YTEREMRHQFKDAGARALVYLNMFgkRVQEVLPDTGIEYLIEAK-MGDLLPMAKGWLINTVVDKVKKMVPayhlpqaisf 187
Cdd:pfam00501 80 LPAEELAYILEDSGAKVLITDDAL--KLEELLEALGKLEVVKLVlVLDRDPVLKEEPLPEEAKPADVPPP---------- 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 kqalregaalshKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGqklikeGQEVMIAPLPL 267
Cdd:pfam00501 148 ------------PPPPPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIKRVRPRGFGLG------PDDRVLSTLPL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIYAFTANCMCMMVTGNHNVLI--TNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTAL 345
Cdd:pfam00501 210 FHDFGLSLGLLGPLLAGATVVLPpgFPALDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPL 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWESLTGCRIVEGYGLTETSPVASTNPYGE--LARLGTVGIPVADTAFKVIDDE-GNELPLGERGELCIKGPQV 422
Cdd:pfam00501 290 PPELARRFRELFGGALVNGYGLTETTGVVTTPLPLDedLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGV 369
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 820875961 423 MKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS 470
Cdd:pfam00501 370 MKGYLNDPELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
23-560 |
1.12e-132 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 396.48 E-value: 1.12e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 23 YTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:PRK06187 5 PLTIGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRA-LGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDtgieylieakmgdlLPMAKGWLINTVVDKVKKMVPAYHLp 182
Cdd:PRK06187 84 HPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQ--------------LPTVRTVIVEGDGPAAPLAPEVGEY- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 qaisfkQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMI 262
Cdd:PRK06187 149 ------EELLAAASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLSHRNLFLHSLAVCAWLKLSRDD----------VYL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHIYAFTANCMCMMVtGNHNVLItnPRDIPGFIKEL-GKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSG 341
Cdd:PRK06187 213 VIVPMFHVHAWGLPYLALMA-GAKQVIP--RRFDPENLLDLiETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIYG 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPY-----GELARLGTVGIPVADTAFKVIDDEGNELP--LGERGE 414
Cdd:PRK06187 290 GAALPPALLREFKEKFGIDLVQGYGMTETSPVVSVLPPedqlpGQWTKRRSAGRPLPGVEARIVDDDGDELPpdGGEVGE 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 415 LCIKGPQVMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:PRK06187 370 IIVRGPWLMQGYWNRPEATAETID-GGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAV 448
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 495 IGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK06187 449 IGVPDEKWGERPVAVVVLKPGAtLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLRE 515
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
6-558 |
1.26e-132 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 398.25 E-value: 1.26e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKRPAGVPSTID-----IHAYtsvvevFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAV 80
Cdd:PRK06710 7 WLKSYPEEIPSTISydiqpLHKY------VEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQK-LGVEKGDRVAI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 81 QMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMA 160
Cdd:PRK06710 80 MLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLDLVFPRVTNVQSATKIEHVIVTRIADFLPFP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 161 KGWLINTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVL 240
Cdd:PRK06710 160 KNLLYPFVQKKQSNLVVKVSESETIHLWNSVEKEVNTGVEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLVSNTLMGV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 241 ACFSQhgpdgqklIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLItnPR-DIPGFIKELGKWRFTALLGLNTLFV 319
Cdd:PRK06710 240 QWLYN--------CKEGEEVVLGVLPFFHVYGMTAVMNLSIMQGYKMVLI--PKfDMKMVFEAIKKHKVTLFPGAPTIYI 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 320 ALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKV 399
Cdd:PRK06710 310 ALLNSPLLKEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSPVTHSNFLWEKRVPGSIGVPWPDTEAMI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 400 IDDE-GNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNE 478
Cdd:PRK06710 390 MSLEtGEALPPGEIGEIVVKGPQIMKGYWNKPEETAAVLQ-DGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPRE 468
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 479 IEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGL-SVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRE 557
Cdd:PRK06710 469 VEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTEcSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRV 548
|
.
gi 820875961 558 L 558
Cdd:PRK06710 549 L 549
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
14-560 |
2.30e-131 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 394.33 E-value: 2.30e-131
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 14 VPSTIDIHAyTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVF 93
Cdd:PRK08314 1 LPKSLTLPE-TSLFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQECGVRKGDRVLLYMQNSPQFVIAYY 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 94 GALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMAKG-----WLintv 168
Cdd:PRK08314 80 AILRANAVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYLPAEPEiavpaWL---- 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 169 vdKVKKMVPAYHLPQAISFKQALREGAALshKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVlACFSQHGP 248
Cdd:PRK08314 156 --RAEPPLQALAPGGVVAWKEALAAGLAP--PPHTAGPDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGS-VLWSNSTP 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 249 DGqklikegqeVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITN-PRDIPGfiKELGKWRFTALLGLNTLFVALMDHPGF 327
Cdd:PRK08314 231 ES---------VVLAVLPLFHVTGMVHSMNAPIYAGATVVLMPRwDREAAA--RLIERYRVTHWTNIPTMVVDFLASPGL 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 328 RQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVID-DEGNE 406
Cdd:PRK08314 300 AERDLSSLRYIGGGGAAMPEAVAERLKELTGLDYVEGYGLTETMAQTHSNPPDR-PKLQCLGIPTFGVDARVIDpETLEE 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 407 LPLGERGELCIKGPQVMKGYWQQPEATAQ---TLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVI 483
Cdd:PRK08314 379 LPPGEVGEIVVHGPQVFKGYWNRPEATAEafiEIDGKRFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLL 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 484 MGHPKVANCAAIGVPDERSGEAVKLFVVAR---EGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK08314 459 YKHPAIQEACVIATPDPRRGETVKAVVVLRpeaRGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQE 538
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
30-555 |
9.91e-129 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 383.50 E-value: 9.91e-129
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLY 109
Cdd:cd17631 1 LRRRARRHPDRTALVFGGRSLTYAELDERVNRLAHALR-ALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 110 TEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkq 189
Cdd:cd17631 80 TPPEVAYILADSGAKVL--------------------------------------------------------------- 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 190 alregaalshkpvtlsLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhGPDgqklikegqEVMIAPLPLYH 269
Cdd:cd17631 97 ----------------FDDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDL-GPD---------DVLLVVAPLFH 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 IYAftANCMCMMVT--GNHNVLITNPRdiPG-FIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALV 346
Cdd:cd17631 151 IGG--LGVFTLPTLlrGGTVVILRKFD--PEtVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGAPMP 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 347 KATAERWESlTGCRIVEGYGLTETSPVASTN-PYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKG 425
Cdd:cd17631 227 ERLLRALQA-RGVKFVQGYGMTETSPGVTFLsPEDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRGPHVMAG 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 426 YWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEA 505
Cdd:cd17631 306 YWNRPEATAAAF-RDGWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEA 384
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 820875961 506 VKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd17631 385 VVAVVVPRPGAeLDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
47-554 |
3.39e-125 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 376.17 E-value: 3.39e-125
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARAL 126
Cdd:cd05911 8 GKELTYAQLRTLSRRLAAGLRKL-GLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VylnmfgkrvqeVLPDtGIEYLIEAkmgdllpmAKGWL----INTVVDKVKKMVPAYHLPQAisfkqalREGAALSHKPV 202
Cdd:cd05911 87 F-----------TDPD-GLEKVKEA--------AKELGpkdkIIVLDDKPDGVLSIEDLLSP-------TLGEEDEDLPP 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 203 TL--SLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHgpdgqkliKEGQEVMIAPLPLYHIYAFTAnCMCM 280
Cdd:cd05911 140 PLkdGKDDTAAILYSSGTTGLPKGVCLSHRNLIANLSQVQTFLYGN--------DGSNDVILGFLPLYHIYGLFT-TLAS 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 281 MVTGnHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESL-TGC 359
Cdd:cd05911 211 LLNG-ATVIIMPKFDSELFLDLIEKYKITFLYLVPPIAAALAKSPLLDKYDLSSLRVILSGGAPLSKELQELLAKRfPNA 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 360 RIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIDDEGNE-LPLGERGELCIKGPQVMKGYWQQPEATAQTLD 438
Cdd:cd05911 290 TIKQGYGMTETGGILTVNPDGD-DKPGSVGRLLPNVEAKIVDDDGKDsLGPNEPGEICVRGPQVMKGYYNNPEATKETFD 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 439 ADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-L 517
Cdd:cd05911 369 EDGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEkL 448
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 820875961 518 SVDELKAYCKANFTGYKvpKH---IVLRESLPMTPVGKIL 554
Cdd:cd05911 449 TEKEVKDYVAKKVASYK--QLrggVVFVDEIPKSASGKIL 486
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
2-558 |
1.37e-123 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 374.76 E-value: 1.37e-123
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 2 QADFWndkrPAGVPSTIDI-HAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAV 80
Cdd:PRK06178 14 QQAAW----PAGIPREPEYpHGERPLTEYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQR-GVGAGDRVAV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 81 QMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMA 160
Cdd:PRK06178 89 FLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAEVLLALDQLAPVVEQVRAETSLRHVIVTSLADVLPAE 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 161 KgwlinTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVL 240
Cdd:PRK06178 169 P-----TLPLPDSLRAPRLAAAGAIDLLPALRACTAPVPLPPP-ALDALAALNYTGGTTGMPKGCEHTQRDMVYTAAAAY 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 241 ACFSQHGPDgqklikegqEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITnpR-DIPGFIKELGKWRFTALLGLNTLFV 319
Cdd:PRK06178 243 AVAVVGGED---------SVFLSFLPEFWIAGENFGLLFPLFSGATLVLLA--RwDAVAFMAAVERYRVTRTVMLVDNAV 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 320 ALMDHPGFRQLDFSALKVTN--SGGTALVKATAERWESLTGCRIVEG-YGLTETSPVAS------TNPYGELARLGTVGI 390
Cdd:PRK06178 312 ELMDHPRFAEYDLSSLRQVRvvSFVKKLNPDYRQRWRALTGSVLAEAaWGMTETHTCDTftagfqDDDFDLLSQPVFVGL 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 391 PVADTAFKVID-DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIV 469
Cdd:PRK06178 392 PVPGTEFKICDfETGELLPLGAEGEIVVRTPSLLKGYWNKPEATAEAL-RDGWLHTGDIGKIDEQGFLHYLGRRKEMLKV 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 470 SGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKhIVLRESLPMT 548
Cdd:PRK06178 471 NGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGaDLTAAALQAWCRENMAVYKVPE-IRIVDALPMT 549
|
570
....*....|
gi 820875961 549 PVGKILRREL 558
Cdd:PRK06178 550 ATGKVRKQDL 559
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
208-554 |
1.05e-119 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 356.60 E-value: 1.05e-119
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANmlqvLACFSQHGPDGQklikegQEVMIAPLPLYHIYAFTANCMCMMVTGNhn 287
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAA----AAALAASGGLTE------GDVFLSTLPLFHIGGLFGLLGALLAGGT-- 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGL 367
Cdd:cd04433 69 VVLLPKFDPEAALELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGL 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 368 TETS-PVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTlDADGWLKTG 446
Cdd:cd04433 149 TETGgTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAV-DEDGWYRTG 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 447 DIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAY 525
Cdd:cd04433 228 DLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGaDLDAEELRAH 307
|
330 340
....*....|....*....|....*....
gi 820875961 526 CKANFTGYKVPKHIVLRESLPMTPVGKIL 554
Cdd:cd04433 308 VRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
29-558 |
3.08e-119 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 361.55 E-value: 3.08e-119
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 29 VFERScKRFADRPAF--SNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTN 106
Cdd:cd05904 11 SFLFA-SAHPSRPALidAATGRALTYAELERRVRRLAAGLA-KRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTAN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 107 PLYTEREMRHQFKDAGARalvylnmfgkrvqevlpdtgieylieakmgdllpmakgwLINTVVDKVKKmVPAYHLP---- 182
Cdd:cd05904 89 PLSTPAEIAKQVKDSGAK---------------------------------------LAFTTAELAEK-LASLALPvvll 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 -----QAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsqhgpdGQKLIKEG 257
Cdd:cd05904 129 dsaefDSLSFSDLLFEADEAEPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVA--------GEGSNSDS 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 258 QEVMIAPLPLYHIYAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKV 337
Cdd:cd05904 201 EDVFLCVLPMFHIYGLSSFALGLLRLGA-TVVVMPRFDLEELLAAIERYKVTHLPVVPPIVLALVKSPIVDKYDLSSLRQ 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 338 TNSGGTALVKATAERW-ESLTGCRIVEGYGLTETSPVAST--NPYGELARLGTVGIPVADTAFKVID-DEGNELPLGERG 413
Cdd:cd05904 280 IMSGAAPLGKELIEAFrAKFPNVDLGQGYGMTESTGVVAMcfAPEKDRAKYGSVGRLVPNVEAKIVDpETGESLPPNQTG 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 414 ELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd05904 360 ELWIRGPSIMKGYLNNPEATAATIDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAA 439
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 494 AIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05904 440 VIPYPDEEAGEVPMAFVVRKPGSsLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
30-562 |
4.58e-108 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 334.47 E-value: 4.58e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPA--FSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:PRK08315 22 LDRTAARYPDREAlvYRDQGLRWTYREFNEEVDALAKGLLAL-GIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALV---------YLNMfgkrVQEVLPDtgieyLIEAKMGDL----LPMAKgWLINTVVDKVKK 174
Cdd:PRK08315 101 AYRLSELEYALNQSGCKALIaadgfkdsdYVAM----LYELAPE-----LATCEPGQLqsarLPELR-RVIFLGDEKHPG 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 175 MvpayhlpqaISFKQALREGAALSHKPV-----TLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACfsqhgpd 249
Cdd:PRK08315 171 M---------LNFDELLALGRAVDDAELaarqaTLDPDDPINIQYTSGTTGFPKGATLTHRNILNNGYFIGEA------- 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 250 gQKLiKEGQEVMIaPLPLYHiyaftancmCM-MVTGN-----HNVLITNPrdIPGF-----IKELGKWRFTALLGLNTLF 318
Cdd:PRK08315 235 -MKL-TEEDRLCI-PVPLYH---------CFgMVLGNlacvtHGATMVYP--GEGFdplatLAAVEEERCTALYGVPTMF 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 319 VALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCR-IVEGYGLTETSPV----ASTNPYgELaRLGTVGIPVA 393
Cdd:PRK08315 301 IAELDHPDFARFDLSSLRTGIMAGSPCPIEVMKRVIDKMHMSeVTIAYGMTETSPVstqtRTDDPL-EK-RVTTVGRALP 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 394 DTAFKVIDDE-GNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGF 472
Cdd:PRK08315 379 HLEVKIVDPEtGETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGE 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 473 NVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVG 551
Cdd:PRK08315 459 NIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGAtLTEEDVRDFCRGKIAHYKIPRYIRFVDEFPMTVTG 538
|
570
....*....|.
gi 820875961 552 KILRRELRDIA 562
Cdd:PRK08315 539 KIQKFKMREMM 549
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
25-560 |
8.34e-106 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 327.66 E-value: 8.34e-106
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVN 104
Cdd:PRK08316 12 TIGDILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLD-LGLKKGDRVAALGHNSDAYALLWLACARAGAVHVP 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 105 TNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAKMGDLLPMAKGWLintvvdkvkkmvpayhlpqa 184
Cdd:PRK08316 91 VNFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLGGREAPGGWL-------------------- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 185 iSFKQALREGAAlSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQ-VLAC-FSQHgpdgqklikegqEVMI 262
Cdd:PRK08316 151 -DFADWAEAGSV-AEPDVELADDDLAQILYTSGTESLPKGAMLTHRALIAEYVScIVAGdMSAD------------DIPL 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHiyafTANCMCMMVT----GNHNVLITNPrDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVT 338
Cdd:PRK08316 217 HALPLYH----CAQLDVFLGPylyvGATNVILDAP-DPELILRTIEAERITSFFAPPTVWISLLRHPDFDTRDLSSLRKG 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 339 NSGGTAL-VKATAERWESLTGCRIVEGYGLTETSPVAST-NPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELC 416
Cdd:PRK08316 292 YYGASIMpVEVLKELRERLPGLRFYNCYGQTEIAPLATVlGPEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEIV 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIG 496
Cdd:PRK08316 372 HRSPQLMLGYWDDPEKTAEAF-RGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIG 450
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 497 VPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK08316 451 LPDPKWIEAVTAVVVPKAGAtVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
28-562 |
1.09e-104 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 325.96 E-value: 1.09e-104
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPA--FSNLGKTLTYGEL----DRLSAAFAAWLQQhtdlvPGDRIAVQMPNVLQYPIAVFGALRAGLI 101
Cdd:PRK12583 22 DAFDATVARFPDREAlvVRHQALRYTWRQLadavDRLARGLLALGVQ-----PGDRVGIWAPNCAEWLLTQFATARIGAI 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 102 VVNTNPLYTEREMRHQFKDAGARALV---------YLNMFGKRVQEvLPDTGIEYLIEAKmgdlLPMAKGwlintVVDKV 172
Cdd:PRK12583 97 LVNINPAYRASELEYALGQSGVRWVIcadafktsdYHAMLQELLPG-LAEGQPGALACER----LPELRG-----VVSLA 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 173 KKMVPAYHLPQAIsfkQALREG---AALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVlacfsqhgpd 249
Cdd:PRK12583 167 PAPPPGFLAWHEL---QARGETvsrEALAERQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFV---------- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 250 GQKLIKEGQEVMIAPLPLYHIYAFT-ANCMCMMVtGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFR 328
Cdd:PRK12583 234 AESLGLTEHDRLCVPVPLYHCFGMVlANLGCMTV-GACLVYPNEAFDPLATLQAVEEERCTALYGVPTMFIAELDHPQRG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 329 QLDFSALKVTNSGGTALVKATAER-WESLTGCRIVEGYGLTETSPVA-STNPYGELA-RLGTVGIPVADTAFKVIDDEGN 405
Cdd:PRK12583 313 NFDLSSLRTGIMAGAPCPIEVMRRvMDEMHMAEVQIAYGMTETSPVSlQTTAADDLErRVETVGRTQPHLEVKVVDPDGA 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMG 485
Cdd:PRK12583 393 TVPRGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFT 472
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 486 HPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK12583 473 HPAVADVQVFGVPDEKYGEEIVAWVRLHPGhAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREIS 550
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
50-558 |
5.20e-103 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 317.11 E-value: 5.20e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVyl 129
Cdd:cd05935 2 LTYLELLEVVKKLASFLSN-KGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAV-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 nmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpVTLSLDDI 209
Cdd:cd05935 79 ------------------------------------------------------------------------VGSELDDL 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 210 AVLQYTGGTTGLAKGAMLTHGNLVANMLQvLACFSQHGPdgqklikegQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVL 289
Cdd:cd05935 87 ALIPYTSGTTGLPKGCMHTHFSAAANALQ-SAVWTGLTP---------SDVILACLPLFHVTGFVGSLNTAVYVGGTYVL 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 290 IT--NPRDIPGFIKelgKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGL 367
Cdd:cd05935 157 MArwDRETALELIE---KYKVTFWTNIPTMLVDLLATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGL 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 368 TETSPVASTNPYGELaRLGTVGIPVADTAFKVIDDE-GNELPLGERGELCIKGPQVMKGYWQQPEATAQ---TLDADGWL 443
Cdd:cd05935 234 TETMSQTHTNPPLRP-KLQCLGIP*FGVDARVIDIEtGRELPPNEVGEIVVRGPQIFKGYWNRPEETEEsfiEIKGRRFF 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 444 KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVARE---GGLSVD 520
Cdd:cd05935 313 RTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPeyrGKVTEE 392
|
490 500 510
....*....|....*....|....*....|....*...
gi 820875961 521 ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05935 393 DIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
28-562 |
5.24e-101 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 316.28 E-value: 5.24e-101
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAF-----SNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:COG0365 13 NCLDRHAEGRGDKVALiwegeDGEERTLTYAELRREVNRFANALRAL-GVKKGDRVAIYLPNIPEAVIAMLACARIGAVH 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARALVylnmfgkrVQEVLPDTGIEYLIEAKMGDLLPMAKGwLINTVVdkVKKMVPAYHLP 182
Cdd:COG0365 92 SPVFPGFGAEALADRIEDAEAKVLI--------TADGGLRGGKVIDLKEKVDEALEELPS-LEHVIV--VGRTGADVPME 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 QAISFKQALREGAAlSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqvLACFSQHGPDgqklIKEGqEVMI 262
Cdd:COG0365 161 GDLDWDELLAAASA-EFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVH----AATTAKYVLD----LKPG-DVFW 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHIYAFTANCMCMMVTGNHNVL---ITNPRDIPGFIKELGKWRFTALLGLNTLFVALM---DHPGfRQLDFSALK 336
Cdd:COG0365 231 CTADIGWATGHSYIVYGPLLNGATVVLyegRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMkagDEPL-KKYDLSSLR 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALVKATAERWESLTGCRIVEGYGLTET-SPVASTNPYGELaRLGTVGIPVADTAFKVIDDEGNELPLGERGEL 415
Cdd:COG0365 310 LLGSAGEPLNPEVWEWWYEAVGVPIVDGWGQTETgGIFISNLPGLPV-KPGSMGKPVPGYDVAVVDEDGNPVPPGEEGEL 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 416 CIKGPQ--VMKGYWQQPEATAQTL--DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVAN 491
Cdd:COG0365 389 VIKGPWpgMFRGYWNDPERYRETYfgRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAE 468
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 492 CAAIGVPDERSGEAVKLFVVAREG-----GLsVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:COG0365 469 AAVVGVPDEIRGQVVKAFVVLKPGvepsdEL-AKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIA 543
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
38-560 |
4.67e-99 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 309.24 E-value: 4.67e-99
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAF--SNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMR 115
Cdd:cd05926 1 PDAPALvvPGSTPALTYADLAELVDDLARQLAAL-GIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 116 HQFKDAGARALVylnmfgkrvqevLPDTGIEYLIEAKMGDLLPmakgwLINTVVDKVKKMVPayhlPQAISFKQALREGA 195
Cdd:cd05926 80 FYLADLGSKLVL------------TPKGELGPASRAASKLGLA-----ILELALDVGVLIRA----PSAESLSNLLADKK 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 196 ALSHKPVTLSlDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsQHGPDGQKLIkegqeVMiaplPLYHIYAFTA 275
Cdd:cd05926 139 NAKSEGVPLP-DDLALILHTSGTTGRPKGVPLTHRNLAASATNITNTY-KLTPDDRTLV-----VM----PLFHVHGLVA 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 276 NCMCMMVTGNhNVLITnprdiPGF--------IKELGKWRFTALLGLNTLFvaLMDHPGFRQLDFSALKVTNSGGTALVK 347
Cdd:cd05926 208 SLLSTLAAGG-SVVLP-----PRFsastfwpdVRDYNATWYTAVPTIHQIL--LNRPEPNPESPPPKLRFIRSCSASLPP 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 348 ATAERWESLTGCRIVEGYGLTETSPVASTNPYGELAR-LGTVGIPVAdTAFKVIDDEGNELPLGERGELCIKGPQVMKGY 426
Cdd:cd05926 280 AVLEALEATFGAPVLEAYGMTEAAHQMTSNPLPPGPRkPGSVGKPVG-VEVRILDEDGEILPPGVVGEICLRGPNVTRGY 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:cd05926 359 LNNPEANAEAAFKDGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEV 438
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 507 KLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05926 439 AAAVVLREGAsVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
15-559 |
1.87e-97 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 306.14 E-value: 1.87e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 15 PSTIDIHAYTSVVEVFERSCKRFADRPAFSNLGKTLTYGEL-DRLS---AAFAAWlqqhtDLVPGDRIAVQMPNVLQYPI 90
Cdd:PRK06188 3 TMADLLHSGATYGHLLVSALKRYPDRPALVLGDTRLTYGQLaDRISryiQAFEAL-----GLGTGDAVALLSLNRPEVLM 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 91 AVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVY-LNMFGKRVQEVLpdtgieylieakmgdllpmakgwlinTVV 169
Cdd:PRK06188 78 AIGAAQLAGLRRTALHPLGSLDDHAYVLEDAGISTLIVdPAPFVERALALL--------------------------ARV 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 170 DKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSlDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhgPD 249
Cdd:PRK06188 132 PSLKHVLTLGPVPDGVDLLAAAAKFGPAPLVAAALP-PDIAGLAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEW--PA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 250 GQKlikegqevMIAPLPLYHiyAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQ 329
Cdd:PRK06188 209 DPR--------FLMCTPLSH--AGGAFFLPTLLRGG-TVIVLAKFDPAEVLRAIEEQRITATFLVPTMIYALLDHPDLRT 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 330 LDFSALKVTNSGGTALVKAtaerwesltgcRIVEG-----------YGLTETSPVASTNPYGE-----LARLGTVGIPVA 393
Cdd:PRK06188 278 RDLSSLETVYYGASPMSPV-----------RLAEAierfgpifaqyYGQTEAPMVITYLRKRDhdpddPKRLTSCGRPTP 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 394 DTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFN 473
Cdd:PRK06188 347 GLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAF-RDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFN 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 474 VYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGK 552
Cdd:PRK06188 426 VFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAaVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGK 505
|
....*..
gi 820875961 553 ILRRELR 559
Cdd:PRK06188 506 PDKKALR 512
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
207-559 |
2.56e-96 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 297.27 E-value: 2.56e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVlacfsqhgpdGQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNH 286
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIVNNGYFI----------GERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGAT 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCR-IVEGY 365
Cdd:cd05917 72 MVFPSPSFDPLAVLEAIEKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMKdVTIAY 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 366 GLTETSPV-ASTNPYGEL-ARLGTVGIPVADTAFKVIDDEGNELP-LGERGELCIKGPQVMKGYWQQPEATAQTLDADGW 442
Cdd:cd05917 152 GMTETSPVsTQTRTDDSIeKRVNTVGRIMPHTEAKIVDPEGGIVPpVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDGW 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 443 LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDE 521
Cdd:cd05917 232 LHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAeLTEED 311
|
330 340 350
....*....|....*....|....*....|....*...
gi 820875961 522 LKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05917 312 IKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
39-560 |
3.83e-94 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 294.58 E-value: 3.83e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaals 198
Cdd:cd05941 81 TDSEPSLVL----------------------------------------------------------------------- 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hkpvtlsldDIAVLQYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQHGPDgqklikegqEVMIAPLPLYHIYAFTANCM 278
Cdd:cd05941 90 ---------DPALILYTSGTTGRPKGVVLTHANL-AANVRALVDAWRWTED---------DVLLHVLPLHHVHGLVNALL 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLITNPRDIPGFIKELGKwRFTALLGLNTLFVALMDHP--------GFRQLDFSALKVTNSGGTALVKATA 350
Cdd:cd05941 151 CPLFAGASVEFLPKFDPKEVAISRLMP-SITVFMGVPTIYTRLLQYYeahftdpqFARAAAAERLRLMVSGSAALPVPTL 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 351 ERWESLTGCRIVEGYGLTETSpVASTNPYGELARLGTVGIPVADTAFKVIDDEGNE-LPLGERGELCIKGPQVMKGYWQQ 429
Cdd:cd05941 230 EEWEAITGHTLLERYGMTEIG-MALSNPLDGERRPGTVGMPLPGVQARIVDEETGEpLPRGEVGEIQVRGPSVFKEYWNK 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 430 PEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKL 508
Cdd:cd05941 309 PEATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSSVDIIKSgGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVA 388
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 820875961 509 FVVAREGG--LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05941 389 VVVLRAGAaaLSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
19-527 |
8.45e-94 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 298.94 E-value: 8.45e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 19 DIHAYTSVVEVFERSCKRFADRPAFSNLG----KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFG 94
Cdd:COG1022 6 DVPPADTLPDLLRRRAARFPDRVALREKEdgiwQSLTWAEFAERVRALAAGLLAL-GVKPGDRVAILSDNRPEWVIADLA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 95 ALRAGLIVVntnPLY---TEREMRHQFKDAGARALV------YlnmfgKRVQEVLPDT-GIEYLIeakmgdllpmakgwl 164
Cdd:COG1022 85 ILAAGAVTV---PIYptsSAEEVAYILNDSGAKVLFvedqeqL-----DKLLEVRDELpSLRHIV--------------- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 165 intVVDKVkkmvPAYHLPQAISFKQALREGAALSHK------PVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQ 238
Cdd:COG1022 142 ---VLDPR----GLRDDPRLLSLDELLALGREVADPaelearRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARA 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 239 VLACFSqhgpdgqklIKEGqEVMIAPLPLYHIYAFTANCMCMM--VTGNH----------------NVLITNPR------ 294
Cdd:COG1022 215 LLERLP---------LGPG-DRTLSFLPLAHVFERTVSYYALAagATVAFaespdtlaedlrevkpTFMLAVPRvwekvy 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 295 --------DIPGFIK-------ELGKWRFTALLGLNTlfVALMDHPGFRQLD---FSALK--------VTNSGGTALVKA 348
Cdd:COG1022 285 agiqakaeEAGGLKRklfrwalAVGRRYARARLAGKS--PSLLLRLKHALADklvFSKLRealggrlrFAVSGGAALGPE 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 349 TAERWESLtGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAFKviddegnelpLGERGELCIKGPQVMKGYWQ 428
Cdd:COG1022 363 LARFFRAL-GIPVLEGYGLTETSPVITVNRPGDN-RIGTVGPPLPGVEVK----------IAEDGEILVRGPNVMKGYYK 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 429 QPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANCAAIG----------V 497
Cdd:COG1022 431 NPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVVGdgrpflaaliV 510
|
570 580 590
....*....|....*....|....*....|
gi 820875961 498 PDErsgEAVKLFvvAREGGLSVDELKAYCK 527
Cdd:COG1022 511 PDF---EALGEW--AEENGLPYTSYAELAQ 535
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
24-559 |
7.78e-92 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 294.56 E-value: 7.78e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFSNL--------GKTLTYGELDRlSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGA 95
Cdd:PRK07529 25 ASTYELLSRAAARHPDAPALSFLldadpldrPETWTYAELLA-DVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGG 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 96 LRAGlIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGK-----RVQEVLPD-TGIEYLIEAKMGDLLPMAKGWLINTVV 169
Cdd:PRK07529 104 EAAG-IANPINPLLEPEQIAELLRAAGAKVLVTLGPFPGtdiwqKVAEVLAAlPELRTVVEVDLARYLPGPKRLAVPLIR 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 170 DKVKKMVPAYhlpqaisfkqalreGAALSHKPVTL-------SLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMlQVLAC 242
Cdd:PRK07529 183 RKAHARILDF--------------DAELARQPGDRlfsgrpiGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANA-WLGAL 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 243 FSQHGPDgqklikegqEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLIT-----NPRDIPGFIKELGKWRFTALLGLNTL 317
Cdd:PRK07529 248 LLGLGPG---------DTVFCGLPLFHVNALLVTGLAPLARGAHVVLATpqgyrGPGVIANFWKIVERYRINFLSGVPTV 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 318 FVALMDHPGFRQlDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAF 397
Cdd:PRK07529 319 YAALLQVPVDGH-DISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEATCVSSVNPPDGERRIGSVGLRLPYQRV 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 398 KVI--DDEGN---ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGF 472
Cdd:PRK07529 398 RVVilDDAGRylrDCAVDEVGVLCIAGPNVFSGYLEAAHNKGLWLE-DGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGH 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 473 NVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTG-YKVPKHIVLRESLPMTPV 550
Cdd:PRK07529 477 NIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGaSATEAELLAFARDHIAErAAVPKHVRILDALPKTAV 556
|
....*....
gi 820875961 551 GKILRRELR 559
Cdd:PRK07529 557 GKIFKPALR 565
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
24-559 |
8.24e-90 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 287.03 E-value: 8.24e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFS-NLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:PRK06087 23 ASLADYWQQTARAMPDKIAVVdNHGASYTYSALDHAASRLANWLLA-KGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVS 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARALVYLNMFGKR--VQEVLPdtgieylieakMGDLLPMAKGWLIntvVDKVKKMVPAyh 180
Cdd:PRK06087 102 VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTrpVDLILP-----------LQNQLPQLQQIVG---VDKLAPATSS-- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 181 lpqaISFKQALREGAALSHkPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsqhgpdgqKLIKEGQEV 260
Cdd:PRK06087 166 ----LSLSQIIADYEPLTT-AITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASERAYCA----------RLNLTWQDV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 261 MIAPLPLYHIYAFTANCMCMMVTGNHNVL--ITNPRDIpgfIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVT 338
Cdd:PRK06087 231 FMMPAPLGHATGFLHGVTAPFLIGARSVLldIFTPDAC---LALLEQQRCTCMLGATPFIYDLLNLLEKQPADLSALRFF 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 339 NSGGTALVKATAER-WESltGCRIVEGYGLTETSPVASTNPYGELARLG-TVGIPVADTAFKVIDDEGNELPLGERGELC 416
Cdd:PRK06087 308 LCGGTTIPKKVAREcQQR--GIKLLSVYGSTESSPHAVVNLDDPLSRFMhTDGYAAAGVEIKVVDEARKTLPPGCEGEEA 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIG 496
Cdd:PRK06087 386 SRGPNVFMGYLDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVA 465
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 497 VPDERSGEAVKLFVV--AREGGLSVDELKAY-CKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK06087 466 MPDERLGERSCAYVVlkAPHHSLTLEEVVAFfSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLR 531
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
28-559 |
4.82e-89 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 284.35 E-value: 4.82e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:COG1021 29 DLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLAL-GLRPGDRVVVQLPNVAEFVIVFFALFRAGAIPVFALP 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALVYLNMFGKRvqevlpdtgieylieakmgDLLPMAkgwlintvvDKVKKMVPayHLPQAISF 187
Cdd:COG1021 108 AHRRAEISHFAEQSEAVAYIIPDRHRGF-------------------DYRALA---------RELQAEVP--SLRHVLVV 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 KQAlREGAALSH---KPVTLSL-----DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQ-VLAC-FSQHgpdgqklikeg 257
Cdd:COG1021 158 GDA-GEFTSLDAllaAPADLSEprpdpDDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRAsAEICgLDAD----------- 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 258 qEVMIAPLPLYHIYAFTANCM--CMMVtGNHNVLITNPRDIPGF--I-KElgkwRFTALLGLNTLFVALMDHPGFRQLDF 332
Cdd:COG1021 226 -TVYLAALPAAHNFPLSSPGVlgVLYA-GGTVVLAPDPSPDTAFplIeRE----RVTVTALVPPLALLWLDAAERSRYDL 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 333 SALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTEtSPVASTNPY-GELARLGTVGIPV-ADTAFKVIDDEGNELPLG 410
Cdd:COG1021 300 SSLRVLQVGGAKLSPELARRVRPALGCTLQQVFGMAE-GLVNYTRLDdPEEVILTTQGRPIsPDDEVRIVDEDGNPVPPG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 411 ERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVA 490
Cdd:COG1021 379 EVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVH 458
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 491 NCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKA-NFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:COG1021 459 DAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFLRErGLAAFKLPDRLEFVDALPLTAVGKIDKKALR 528
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
30-560 |
9.11e-89 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 282.92 E-value: 9.11e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPA---FSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQmpnVLQYPIAVF---GALRAGLIVV 103
Cdd:PRK07514 6 FDALRAAFADRDApfiETPDGLRYTYGDLDAASARLANLLVA-LGVKPGDRVAVQ---VEKSPEALAlylATLRAGAVFL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 104 NTNPLYTEREMRHQFKDAGARALVYL-NMFG--KRVQEVLPDTGIEYLIEAKMGDLLPMAkgwlintvvdkvkkmvpayh 180
Cdd:PRK07514 82 PLNTAYTLAELDYFIGDAEPALVVCDpANFAwlSKIAAAAGAPHVETLDADGTGSLLEAA-------------------- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 181 lpqaisfkqalrEGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmLQVLACFSQHGPDgqklikegqEV 260
Cdd:PRK07514 142 ------------AAAPDDFETVPRGADDLAAILYTSGTTGRSKGAMLSHGNLLSN-ALTLVDYWRFTPD---------DV 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 261 MIAPLPLYHIYA-FTA-NCMCMmvtgNHNVLITNPR-DIPGFIKELGkwRFTALLGLNTLFVALMDHPGFRQLDFSALKV 337
Cdd:PRK07514 200 LIHALPIFHTHGlFVAtNVALL----AGASMIFLPKfDPDAVLALMP--RATVMMGVPTFYTRLLQEPRLTREAAAHMRL 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 338 TNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVAStNPYGELARLGTVGIPVADTAFKVID-DEGNELPLGERGELC 416
Cdd:PRK07514 274 FISGSAPLLAETHREFQERTGHAILERYGMTETNMNTS-NPYDGERRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIE 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIG 496
Cdd:PRK07514 353 VKGPNVFKGYWRMPEKTAEEFRADGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIG 432
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 497 VPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07514 433 VPHPDFGEGVTAVVVPKPGAaLDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLRE 497
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
51-559 |
2.58e-87 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 276.48 E-value: 2.58e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAAWLQQHTDlVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYln 130
Cdd:cd05934 5 TYAELLRESARIAAALAALGI-RPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV-- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 131 mfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvtlsldDIA 210
Cdd:cd05934 82 -----------------------------------------------------------------------------DPA 84
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 211 VLQYTGGTTGLAKGAMLTHgnlvANMLQVLACFSQHGPDGQKlikegqEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLI 290
Cdd:cd05934 85 SILYTSGTTGPPKGVVITH----ANLTFAGYYSARRFGLGED------DVYLTVLPLFHINAQAVSVLAALSVGATLVLL 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 291 tnPRDIP-GFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTE 369
Cdd:cd05934 155 --PRFSAsRFWSDVRRYGATVTNYLGAMLSYLLAQP--PSPDDRAHRLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTE 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 370 TSpVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIK---GPQVMKGYWQQPEATAQTLdADGWLKTG 446
Cdd:cd05934 231 TI-VGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRglrGWGFFKGYYNMPEATAEAM-RNGWFHTG 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 447 DIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAY 525
Cdd:cd05934 309 DLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGEtLDPEELFAF 388
|
490 500 510
....*....|....*....|....*....|....
gi 820875961 526 CKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05934 389 CEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
14-559 |
2.87e-86 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 277.25 E-value: 2.87e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 14 VPSTIDIHAYtsvveVFERSCKrFADRPAFSN--LGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIA 91
Cdd:PLN02246 19 IPNHLPLHDY-----CFERLSE-FSDRPCLIDgaTGRVYTYADVELLSRRVAAGLHKL-GIRQGDVVMLLLPNCPEFVLA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 92 VFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEylieakmgdllpmakgwlINTVVDK 171
Cdd:PLN02246 92 FLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVT------------------VVTIDDP 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 172 VKKMVPAYHLPQAisfkqalrEGAALShkPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLacfsqhgpDGQ 251
Cdd:PLN02246 154 PEGCLHFSELTQA--------DENELP--EVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQV--------DGE 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 252 --KLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQ 329
Cdd:PLN02246 216 npNLYFHSDDVILCVLPMFHIYSLNSVLLCGLRVGA-AILIMPKFEIGALLELIQRHKVTIAPFVPPIVLAIAKSPVVEK 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 330 LDFSALKVTNSGGTALVKATAERWES-LTGCRIVEGYGLTETSPVASTNpygeLA--------RLGTVGIPVADTAFKVI 400
Cdd:PLN02246 295 YDLSSIRMVLSGAAPLGKELEDAFRAkLPNAVLGQGYGMTEAGPVLAMC----LAfakepfpvKSGSCGTVVRNAELKIV 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 401 D-DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEI 479
Cdd:PLN02246 371 DpETGASLPRNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAEL 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 480 EDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PLN02246 451 EALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGsEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDL 530
|
.
gi 820875961 559 R 559
Cdd:PLN02246 531 R 531
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
49-559 |
2.74e-82 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 263.86 E-value: 2.74e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 49 TLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVY 128
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAA-LGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 129 LNMFGKRVQEVLPDtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvtlsldD 208
Cdd:cd05903 80 PERFRQFDPAAMPD-----------------------------------------------------------------A 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 209 IAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAFTANCMCMMVTGNHNV 288
Cdd:cd05903 95 VALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGLGPGD----------VFLVASPMAHQTGFVYGFTLPLLLGAPVV 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 289 L--ITNPRDIPGFIKELgkwRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYG 366
Cdd:cd05903 165 LqdIWDPDKALALMREH---GVTFMMGATPFLTDLLNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSAYG 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 367 LTET-SPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDaDGWLKT 445
Cdd:cd05903 242 STECpGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRGPSVFLGYLDRPDLTADAAP-EGWFRT 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 446 GDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKA 524
Cdd:cd05903 321 GDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGAlLTFDELVA 400
|
490 500 510
....*....|....*....|....*....|....*.
gi 820875961 525 YC-KANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05903 401 YLdRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLR 436
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
23-559 |
7.68e-82 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 265.00 E-value: 7.68e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 23 YTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:cd05959 3 YNAATLVDLNLNEGRGDKTAFIDDAGSLTYAELEAEARRVAGALRA-LGVKREERVLLIMLDTVDFPTAFLGAIRAGIVP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLpdtgieylieAKMGDLLPMakgwLIntVVDkvkkmvPAYHLP 182
Cdd:cd05959 82 VPVNTLLTPDDYAYYLEDSRARVVVVSGELAPVLAAAL----------TKSEHTLVV----LI--VSG------GAGPEA 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 QAISFKQALREGAAlSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNL--VANML--QVLAcfsqhgpdgqklIKEGQ 258
Cdd:cd05959 140 GALLLAELVAAEAE-QLKPAATHADDPAFWLYSSGSTGRPKGVVHLHADIywTAELYarNVLG------------IREDD 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 259 EVMIAPlPLYHIYAFTANCMCMMVTGNHNVLITNpRDIPG-FIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKV 337
Cdd:cd05959 207 VCFSAA-KLFFAYGLGNSLTFPLSVGATTVLMPE-RPTPAaVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSRDLSSLRL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 338 TNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAFKVIDDEGNELPLGERGELCI 417
Cdd:cd05959 285 CVSAGEALPAEVGERWKARFGLDILDGIGSTEMLHIFLSNRPGRV-RYGTTGKPVPGYEVELRDEDGGDVADGEPGELYV 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 418 KGPQVMKGYWQQPEATAQTLDAdGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGV 497
Cdd:cd05959 364 RGPSSATMYWNNRDKTRDTFQG-EWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGV 442
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 498 PDERSGEAVKLFVVAREG----GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05959 443 EDEDGLTKPKAFVVLRPGyedsEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
39-561 |
2.80e-79 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 257.87 E-value: 2.80e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:PRK06839 17 DRIAIITEEEEMTYKQLHEYVSKVAAYLIYELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVYLNMFGKRVQEVLPDTGIEYLIeakmgdllpmakgWLintvvdkvkkmvpayhlpqaisfkQALREgaALS 198
Cdd:PRK06839 97 KDSGTTVLFVEKTFQNMALSMQKVSYVQRVI-------------SI------------------------TSLKE--IED 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTL---SLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAFTA 275
Cdd:PRK06839 138 RKIDNFvekNESASFIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHD----------RSIVLLPLFHIGGIGL 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 276 NCMCMMVTGNHnVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTA----LVKATAE 351
Cdd:PRK06839 208 FAFPTLFAGGV-IIVPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPcpeeLMREFID 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 352 RwesltGCRIVEGYGLTETSPVASTNPYGELAR-LGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQP 430
Cdd:PRK06839 287 R-----GFLFGQGFGMTETSPTVFMLSEEDARRkVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRP 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 431 EATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV 510
Cdd:PRK06839 362 DATEETIQ-DGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFI 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 820875961 511 VAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK06839 441 VKKSSSvLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
208-555 |
6.30e-77 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 246.26 E-value: 6.30e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHgnlvanmLQVLACFSQHGPDGQklIKEGQEVMIAPlPLYHIYAFTANCMCMMVTGNhN 287
Cdd:cd17638 1 DVSDIMFTSGTTGRSKGVMCAH-------RQTLRAAAAWADCAD--LTEDDRYLIIN-PFFHTFGYKAGIVACLLTGA-T 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIV-EGYG 366
Cdd:cd17638 70 VVPVAVFDVDAILEAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFETVlTAYG 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 367 LTETSPVAstnpygeLARLGTVGIPVADTAFKVIDdeGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTG 446
Cdd:cd17638 150 LTEAGVAT-------MCRPGDDAETVATTCGRACP--GFEVRIADDGEVLVRGYNVMQGYLDDPEATAEAIDADGWLHTG 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 447 DIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAY 525
Cdd:cd17638 221 DVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGvTLTEEDVIAW 300
|
330 340 350
....*....|....*....|....*....|
gi 820875961 526 CKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd17638 301 CRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
35-560 |
9.15e-77 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 252.27 E-value: 9.15e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 35 KRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREM 114
Cdd:PRK07470 18 RRFPDRIALVWGDRSWTWREIDARVDALAAALA-ARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPDEV 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 115 RHQFKDAGARALVYLNMF---GKRVQEVLPDTGIEYLIEAKmgdllpmakgwlintvvdkvkkmvpayhlPQAISFKQAL 191
Cdd:PRK07470 97 AYLAEASGARAMICHADFpehAAAVRAASPDLTHVVAIGGA-----------------------------RAGLDYEALV 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 192 REGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNL---VANMLQVLAcfsqhgPDgqkliKEGQEVMIAPLPLY 268
Cdd:PRK07470 148 ARHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGQMafvITNHLADLM------PG-----TTEQDASLVVAPLS 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 269 H---IYAftancMCMMVTGNHNVLITNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTA 344
Cdd:PRK07470 217 HgagIHQ-----LCQVARGAATVLLPSERfDPAEVWALVERHRVTNLFTVPTILKMLVEHPAVDRYDHSSLRYVIYAGAP 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 345 LVKATAERWESLTGCRIVEGYGLTE-TSPVASTNPY------GELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCI 417
Cdd:PRK07470 292 MYRADQKRALAKLGKVLVQYFGLGEvTGNITVLPPAlhdaedGPDARIGTCGFERTGMEVQIQDDEGRELPPGETGEICV 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 418 KGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGV 497
Cdd:PRK07470 372 IGPAVFAGYYNNPEANAKAF-RDGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGV 450
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 498 PDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07470 451 PDPVWGEVGVAVCVARDGApVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
51-560 |
9.41e-76 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 249.08 E-value: 9.41e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPN-----VLQYPIAVFGAlragliVVNT-NPLYTEREMRHQFKDAGAR 124
Cdd:cd12119 27 TYAEVAERARRLANALRR-LGVKPGDRVATLAWNthrhlELYYAVPGMGA------VLHTiNPRLFPEQIAYIINHAEDR 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 125 ALVYLNMFGKRVQEVLPD-TGIEYLIEAKMGDLLPMAkgwlintvvdkvkkmvpayHLPQAISFKQALREGAALSHKPVt 203
Cdd:cd12119 100 VVFVDRDFLPLLEAIAPRlPTVEHVVVMTDDAAMPEP-------------------AGVGVLAYEELLAAESPEYDWPD- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 204 LSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsqhgPDGQKLikEGQEVMIAPLPLYHIYAFTANCMCMMvT 283
Cdd:cd12119 160 FDENTAAAICYTSGTTGNPKGVVYSHRSLVLHAMAALL------TDGLGL--SESDVVLPVVPMFHVNAWGLPYAAAM-V 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 284 GNHNVLiTNPRDIPGFIKEL-GKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLtGCRIV 362
Cdd:cd12119 231 GAKLVL-PGPYLDPASLAELiEREGVTFAAGVPTVWQGLLDHLEANGRDLSSLRRVVIGGSAVPRSLIEAFEER-GVRVI 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 363 EGYGLTETSPVASTN---------PYGE-LARLGTVGIPVADTAFKVIDDEGNELPL--GERGELCIKGPQVMKGYWQQP 430
Cdd:cd12119 309 HAWGMTETSPLGTVArppsehsnlSEDEqLALRAKQGRPVPGVELRIVDDDGRELPWdgKAVGELQVRGPWVTKSYYKND 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 431 EATAQtLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV 510
Cdd:cd12119 389 EESEA-LTEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVV 467
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 820875961 511 VAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd12119 468 VLKEGAtVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
48-512 |
1.40e-75 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 246.74 E-value: 1.40e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLY---TEREMRHQFKDAGAR 124
Cdd:cd05907 4 QPITWAEFAEEVRALAKGLI-ALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPV---PIYptsSAEQIAYILNDSEAK 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 125 ALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvVDKvkkmvpayhlpqaisfkqalregaalshkpvtl 204
Cdd:cd05907 80 ALF-----------------------------------------VED--------------------------------- 85
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 sLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqVLACFSQHGPDGQklikegqEVMIAPLPLYHIYAFTANCMCMMVTG 284
Cdd:cd05907 86 -PDDLATIIYTSGTTGRPKGVMLSHRNILSN---ALALAERLPATEG-------DRHLSFLPLAHVFERRAGLYVPLLAG 154
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 285 NHNVLITNPRDIPGFIKELgkwRFTALLGLNTLF------VALMDHPGFRQLDF-----SALKVTNSGGTALVKATAERW 353
Cdd:cd05907 155 ARIYFASSAETLLDDLSEV---RPTVFLAVPRVWekvyaaIKVKAVPGLKRKLFdlavgGRLRFAASGGAPLPAELLHFF 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 354 ESLtGCRIVEGYGLTETSPVASTNPyGELARLGTVGIPVadtafkviddEGNELPLGERGELCIKGPQVMKGYWQQPEAT 433
Cdd:cd05907 232 RAL-GIPVYEGYGLTETSAVVTLNP-PGDNRIGTVGKPL----------PGVEVRIADDGEILVRGPNVMLGYYKNPEAT 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 434 AQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANCAAIGvpDERSgeavklFVVA 512
Cdd:cd05907 300 AEALDADGWLHTGDLGEIDEDGFLHITGRKKDLIITSgGKNISPEPIENALKASPLISQAVVIG--DGRP------FLVA 371
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
39-560 |
3.33e-74 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 244.33 E-value: 3.33e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNL--GKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRH 116
Cdd:PRK09088 10 QRLAAVDLalGRRWTYAELDALVGRLAAVLRRR-GCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDA 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 117 QFKDAGARALVylnmfgkrvqevlpdtgieylieakmGDLlPMAKGWLINTVVDKVkkmvpayhlpqaisfkQALREGAA 196
Cdd:PRK09088 89 LLQDAEPRLLL--------------------------GDD-AVAAGRTDVEDLAAF----------------IASADALE 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 197 LSHKPvTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLvanmLQVLACFSQHGPdgqkliKEGQEVMIAPLPLYHIYAFTAN 276
Cdd:PRK09088 126 PADTP-SIPPERVSLILFTSGTSGQPKGVMLSERNL----QQTAHNFGVLGR------VDAHSSFLCDAPMFHIIGLITS 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 277 CMCMMVTGNhNVLITNPRDIPGFIKELGKWRF--TALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGtALVKATAERWE 354
Cdd:PRK09088 195 VRPVLAVGG-SILVSNGFEPKRTLGRLGDPALgiTHYFCVPQMAQAFRAQPGFDAAALRHLTALFTGG-APHAAEDILGW 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 SLTGCRIVEGYGLTETSPV--ASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEA 432
Cdd:PRK09088 273 LDDGIPMVDGFGMSEAGTVfgMSVDCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQA 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 433 TAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVA 512
Cdd:PRK09088 353 TARAFTGDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVP 432
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 820875961 513 REG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK09088 433 ADGaPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRD 481
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
39-559 |
1.28e-73 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 244.30 E-value: 1.28e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:PRK07786 32 DAPALRFLGNTTTWRELDDRVAALAGALSRR-GVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLV 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrVQEVLPDtgieylIEAKMGDLLPmakgwLINTVVdkvkkMVPAYHLPQAISFKQALREGAAlS 198
Cdd:PRK07786 111 SDCGAHVVV--------TEAALAP------VATAVRDIVP-----LLSTVV-----VAGGSSDDSVLGYEDLLAEAGP-A 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVAnmlQVLACFSQHGPDgqklikEGQEVMIAPLPLYHIyAFTANCM 278
Cdd:PRK07786 166 HAPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTG---QAMTCLRTNGAD------INSDVGFVGVPLFHI-AGIGSML 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLI-TNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFsALKVTNSGGT----ALVKATAErw 353
Cdd:PRK07786 236 PGLLLGAPTVIYpLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQARPRDL-ALRVLSWGAApasdTLLRQMAA-- 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 354 eSLTGCRIVEGYGLTETSPVASTnPYGE--LARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPE 431
Cdd:PRK07786 313 -TFPEAQILAAFGQTEMSPVTCM-LLGEdaIRKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPE 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 432 ATAQTLDAdGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVV 511
Cdd:PRK07786 391 ATAEAFAG-GWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAA 469
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 820875961 512 AREGG--LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK07786 470 VRNDDaaLTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELR 519
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
40-559 |
1.46e-73 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 241.23 E-value: 1.46e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 40 RPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMrhqfk 119
Cdd:cd05958 1 RTCLRSPEREWTYRDLLALANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKEL----- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 120 dagaralvylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpAYHLPQAisfkqalREGAALSH 199
Cdd:cd05958 76 ----------------------------------------------------------AYILDKA-------RITVALCA 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 200 KPVTLSlDDIAVLQYTGGTTGLAKGAMLTHgnlvANMLQVLACFSQHgpdgqKLIKEGQEVMIAPLPLYHIYAFTANCMC 279
Cdd:cd05958 91 HALTAS-DDICILAFTSGTTGAPKATMHFH----RDPLASADRYAVN-----VLRLREDDRFVGSPPLAFTFGLGGVLLF 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 280 MMVTGNHNVLI--TNPRDIPGFIkelGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLT 357
Cdd:cd05958 161 PFGVGASGVLLeeATPDLLLSAI---ARYKPTVLFTAPTAYRAMLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEAT 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 358 GCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQvmkGYWQQPEATAQTL 437
Cdd:cd05958 238 GIPIIDGIGSTEMFHIFISARPGD-ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRGPT---GCRYLADKRQRTY 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 438 DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG- 516
Cdd:cd05958 314 VQGGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVi 393
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 820875961 517 ---LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05958 394 pgpVLARELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
37-560 |
9.56e-73 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 242.19 E-value: 9.56e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 37 FADRPAF--SNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREM 114
Cdd:PLN02330 41 YADKVAFveAVTGKAVTYGEVVRDTRRFAKALRS-LGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEI 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 115 RHQFKDAGARALVYLNMFGKRVQevlpdtGIEylieakmgdlLPMakgwlinTVVDKVKkmvpayhLPQAISFKQAL--- 191
Cdd:PLN02330 120 KKQAEAAGAKLIVTNDTNYGKVK------GLG----------LPV-------IVLGEEK-------IEGAVNWKELLeaa 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 192 -REGAALSHKPVTLSldDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLacFSQhgpdGQKLIkeGQEVMIAPLPLYHI 270
Cdd:PLN02330 170 dRAGDTSDNEEILQT--DLCALPFSSGTTGISKGVMLTHRNLVANLCSSL--FSV----GPEMI--GQVVTLGLIPFFHI 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 271 YAFTANCmCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTN--SGGTALVKA 348
Cdd:PLN02330 240 YGITGIC-CATLRNKGKVVVMSRFELRTFLNALITQEVSFAPIVPPIILNLVKNPIVEEFDLSKLKLQAimTAAAPLAPE 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 349 TAERWES-LTGCRIVEGYGLTETSPVASTNPYGE----LARLGTVGIPVADTAFKVID-DEGNELPLGERGELCIKGPQV 422
Cdd:PLN02330 319 LLTAFEAkFPGVQVQEAYGLTEHSCITLTHGDPEkghgIAKKNSVGFILPNLEVKFIDpDTGRSLPKNTPGELCVRSQCV 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 423 MKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERS 502
Cdd:PLN02330 399 MQGYYNNKEETDRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEA 478
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 820875961 503 GE-AVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PLN02330 479 GEiPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
47-560 |
1.41e-71 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 237.49 E-value: 1.41e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARAL 126
Cdd:PRK08276 9 GEVVTYGELEARSNRLAHGLRAL-GLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VylnmfgkrVQEVLPDTGIEYLIEAKMG-DLLPMAKGwlintvvdkvkkmvpayHLPQAISFKQALREGAALSHKPVTLS 205
Cdd:PRK08276 88 I--------VSAALADTAAELAAELPAGvPLLLVVAG-----------------PVPGFRSYEEALAAQPDTPIADETAG 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 206 lddiAVLQYTGGTTGLAKGAM--LTHGNL--VANMLQVLACFSQHGPDGQklikegqeVMIAPLPLYHiyafTA-NCMCM 280
Cdd:PRK08276 143 ----ADMLYSSGTTGRPKGIKrpLPGLDPdeAPGMMLALLGFGMYGGPDS--------VYLSPAPLYH----TApLRFGM 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 281 MV-TGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHP-GFRQ-LDFSALKVTNSGGT----ALVKATAERW 353
Cdd:PRK08276 207 SAlALGGTVVVMEKFDAEEALALIERYRVTHSQLVPTMFVRMLKLPeEVRArYDVSSLRVAIHAAApcpvEVKRAMIDWW 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 354 esltGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVaDTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEAT 433
Cdd:PRK08276 287 ----GPIIHEYYASSEGGGVTVITSEDWLAHPGSVGKAV-LGEVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKT 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 434 AQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAR 513
Cdd:PRK08276 362 AAARNPHGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPA 441
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 820875961 514 EGGLSVD----ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK08276 442 DGADAGDalaaELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLRD 492
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
39-562 |
9.84e-71 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 236.87 E-value: 9.84e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAF------SNLGKTLTYGELDRLSAAFAAWLqqhTDL--VPGDRIAVQMPNVLQYPIAVFGALRAGLIvvnTNPL-- 108
Cdd:PRK13295 39 DKTAVtavrlgTGAPRRFTYRELAALVDRVAVGL---ARLgvGRGDVVSCQLPNWWEFTVLYLACSRIGAV---LNPLmp 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 109 -YTEREMRHQFKDAGARALVYLNMF--------GKRVQEVLPDtgIEYLIeakmgdllpmakgwlintVVDKVKKMVPAY 179
Cdd:PRK13295 113 iFRERELSFMLKHAESKVLVVPKTFrgfdhaamARRLRPELPA--LRHVV------------------VVGGDGADSFEA 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 180 HLPQAiSFKQALREGAAL-SHKPvtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLqvlacfsqhgPDGQKLIKEGQ 258
Cdd:PRK13295 173 LLITP-AWEQEPDAPAILaRLRP---GPDDVTQLIYTSGTTGEPKGVMHTANTLMANIV----------PYAERLGLGAD 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 259 EVMIAPLPLYHIYAFTANCMCMMVTGNHNVL--ITNPRDIPGFIKELGkwrFTALLGLNTLFVALMDHPGFRQLDFSALK 336
Cdd:PRK13295 239 DVILMASPMAHQTGFMYGLMMPVMLGATAVLqdIWDPARAAELIRTEG---VTFTMASTPFLTDLTRAVKESGRPVSSLR 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTV-GIPVADTAFKVIDDEGNELPLGERGEL 415
Cdd:PRK13295 316 TFLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTLTKLDDPDERASTTdGCPLPGVEVRVVDADGAPLPAGQIGRL 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 416 CIKGPQVMKGYWQQPEATAQtlDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAI 495
Cdd:PRK13295 396 QVRGCSNFGGYLKRPQLNGT--DADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIV 473
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 496 GVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANftgyKV-----PKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK13295 474 AYPDERLGERACAFVVPRPGqSLDFEEMVEFLKAQ----KVakqyiPERLVVRDALPRTPSGKIQKFRLREML 542
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
47-561 |
9.91e-71 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 237.05 E-value: 9.91e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGArAL 126
Cdd:PLN02574 64 GFSISYSELQPLVKSMAAGLYHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSV-GL 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VYlnmfgkrvqevlpdtgieylieakmgdllpmakgwlinTVVDKVKKM----VPAYHLPQAISFKQALREGAALSH--- 199
Cdd:PLN02574 143 AF--------------------------------------TSPENVEKLsplgVPVIGVPENYDFDSKRIEFPKFYElik 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 200 -------KPVtLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVAnMLQVLACF--SQHGPDGQklikegQEVMIAPLPLYHI 270
Cdd:PLN02574 185 edfdfvpKPV-IKQDDVAAIMYSSGTTGASKGVVLTHRNLIA-MVELFVRFeaSQYEYPGS------DNVYLAALPMFHI 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 271 YAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHP-GFRQLDFSALKVTNSGGTALVKAT 349
Cdd:PLN02574 257 YGLSLFVVGLLSLGS-TIVVMRRFDASDMVKVIDRFKVTHFPVVPPILMALTKKAkGVCGEVLKSLKQVSCGAAPLSGKF 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 350 AERW-ESLTGCRIVEGYGLTETSPVASTNPYGE-LARLGTVGIPVADTAFKVID-DEGNELPLGERGELCIKGPQVMKGY 426
Cdd:PLN02574 336 IQDFvQTLPHVDFIQGYGMTESTAVGTRGFNTEkLSKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQGPGVMKGY 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:PLN02574 416 LNNPKATQSTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIP 495
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 507 KLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PLN02574 496 VAFVVRRQGsTLSQEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRS 551
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
75-559 |
1.88e-70 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 234.96 E-value: 1.88e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 75 GDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIeylieakmg 154
Cdd:PRK08008 62 GDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDAT--------- 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 155 dllPMAKGWLINTVVDKVkkmvpayhlPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVa 234
Cdd:PRK08008 133 ---PLRHICLTRVALPAD---------DGVSSFTQLKAQQPATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLR- 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 235 nmlqvlacFSQHGPDGQKLIKEgQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLIT--NPRdipGFIKELGKWRFTALL 312
Cdd:PRK08008 200 --------FAGYYSAWQCALRD-DDVYLTVMPAFHIDCQCTAAMAAFSAGATFVLLEkySAR---AFWGQVCKYRATITE 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 313 GLNTLFVALMDHPG--------FRQLDFSaLKVTNsggtalvkATAERWESLTGCRIVEGYGLTETSPVASTNPYGELAR 384
Cdd:PRK08008 268 CIPMMIRTLMVQPPsandrqhcLREVMFY-LNLSD--------QEKDAFEERFGVRLLTSYGMTETIVGIIGDRPGDKRR 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 385 LGTVGIPVADTAFKVIDDEGNELPLGERGELCIKG---PQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVD 461
Cdd:PRK08008 339 WPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGvpgKTIFKEYYLDPKATAKVLEADGWLHTGDTGYVDEEGFFYFVD 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 462 RKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIV 540
Cdd:PRK08008 419 RRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGeTLSEEEFFAFCEQNMAKFKVPSYLE 498
|
490
....*....|....*....
gi 820875961 541 LRESLPMTPVGKILRRELR 559
Cdd:PRK08008 499 IRKDLPRNCSGKIIKKNLK 517
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
50-560 |
7.14e-70 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 231.07 E-value: 7.14e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYl 129
Cdd:cd05972 1 WSFRELKRESAKAANVLAKL-GLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 nmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvtlSLDDI 209
Cdd:cd05972 79 ---------------------------------------------------------------------------DAEDP 83
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 210 AVLQYTGGTTGLAKGAMLTHGNLVAnmlqvlacfsqHGPDGQKL--IKEGqEVMIAPLPLYHIYAFTANCMCMMVTGNHN 287
Cdd:cd05972 84 ALIYFTSGTTGLPKGVLHTHSYPLG-----------HIPTAAYWlgLRPD-DIHWNIADPGWAKGAWSSFFGPWLLGATV 151
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDhPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYG 366
Cdd:cd05972 152 FVYEGPRfDAERILELLERYGVTSFCGPPTAYRMLIK-QDLSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYG 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 367 LTETSPVASTNPYGELaRLGTVGIPVADTAFKVIDDEGNELPLGERGELCIK--GPQVMKGYWQQPEATAQTLdADGWLK 444
Cdd:cd05972 231 QTETGLTVGNFPDMPV-KPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKlpPPGLFLGYVGDPEKTEASI-RGDYYL 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 445 TGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLS----VD 520
Cdd:cd05972 309 TGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPseelAE 388
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 820875961 521 ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05972 389 ELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
40-559 |
1.57e-69 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 230.43 E-value: 1.57e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 40 RPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFK 119
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLG-VSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIAR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 120 DAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalsh 199
Cdd:cd05919 80 DCEARLVV------------------------------------------------------------------------ 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 200 kpvtLSLDDIAVLQYTGGTTGLAKGAMLTHGNLV----ANMLQVLAcfsqhgpdgqklIKEGQEVMIAPlPLYHIYAFTA 275
Cdd:cd05919 88 ----TSADDIAYLLYSSGTTGPPKGVMHAHRDPLlfadAMAREALG------------LTPGDRVFSSA-KMFFGYGLGN 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 276 NCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWES 355
Cdd:cd05919 151 SLWFPLAVGASAVLNPGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVSAGEALPRGLGERWME 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LTGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQ 435
Cdd:cd05919 231 HFGGPILDGIGATEVGHIFLSNRPGA-WRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRA 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 436 TLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG 515
Cdd:cd05919 310 TF-NGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSP 388
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 820875961 516 ----GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05919 389 aapqESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
43-562 |
2.15e-69 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 231.45 E-value: 2.15e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 43 FSNLGKTLTYGELDRLSAAFAAWLQQHTdlVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAG 122
Cdd:cd05909 1 EDTLGTSLTYRKLLTGAIALARKLAKMT--KEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 123 ARALVYLNMFgkrvqevlpdtgIEYLIEAKMGDLLPMAKgwliNTVVDKVKKmvpayhlpqAISFKQALREGAALSHKP- 201
Cdd:cd05909 79 IKTVLTSKQF------------IEKLKLHHLFDVEYDAR----IVYLEDLRA---------KISKADKCKAFLAGKFPPk 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 202 --------VTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAF 273
Cdd:cd05909 134 wllrifgvAPVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPED----------VVFGALPFFHSFGL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 274 TANCMCMMVTGNHNVLITNP---RDIPGFIKELGKwrfTALLGLNTLFVALMD--HPGfrqlDFSALKVTNSGGTALVKA 348
Cdd:cd05909 204 TGCLWLPLLSGIKVVFHPNPldyKKIPELIYDKKA---TILLGTPTFLRGYARaaHPE----DFSSLRLVVAGAEKLKDT 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 349 TAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGN-ELPLGERGELCIKGPQVMKGYW 427
Cdd:cd05909 277 LRQEFQEKFGIRILEGYGTTECSPVISVNTPQSPNKEGTVGRPLPGMEVKIVSVETHeEVPIGEGGLLLVRGPNVMLGYL 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 428 QQPEATaQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGH-PKVANCAAIGVPDERSGEAV 506
Cdd:cd05909 357 NEPELT-SFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKI 435
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 507 KLFVVAREggLSVDELKAYCKA----NFTgykVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05909 436 VLLTTTTD--TDPSSLNDILKNagisNLA---KPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
29-561 |
5.73e-68 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 229.26 E-value: 5.73e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 29 VFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPL 108
Cdd:PRK06155 26 MLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAA-GVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTA 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 109 YTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDtgieylieakmgdLLPMAKGWLINTVvdkvkkmvPAYHLPQAISFK 188
Cdd:PRK06155 105 LRGPQLEHILRNSGARLLVVEAALLAALEAADPG-------------DLPLPAVWLLDAP--------ASVSVPAGWSTA 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 189 QALREGAALShkPVTLSLDDIAVLQYTGGTTGLAKGAMLTH------GNLVANMLQVlacfsqhGPDgqklikegqEVMI 262
Cdd:PRK06155 164 PLPPLDAPAP--AAAVQPGDTAAILYTSGTTGPSKGVCCPHaqfywwGRNSAEDLEI-------GAD---------DVLY 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHIYAFTAnCMCMMVTGNHNVLitNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSG 341
Cdd:PRK06155 226 TTLPLFHTNALNA-FFQALLAGATYVL--EPRfSASGFWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHRVRVALGP 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GT--ALVKATAERweslTGCRIVEGYGLTET-SPVASTNPYgelARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIK 418
Cdd:PRK06155 303 GVpaALHAAFRER----FGVDLLDGYGSTETnFVIAVTHGS---QRPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLR 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 419 GPQ---VMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAI 495
Cdd:PRK06155 376 ADEpfaFATGYFGMPEKTVEAWR-NLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVF 454
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 496 GVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK06155 455 PVPSELGEDEVMAAVVLRDGtALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
207-562 |
1.13e-67 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 223.13 E-value: 1.13e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVAN--MLQVLACFsqhGPDgqklikegqEVMIAPLPLYHIYAFTANCMCMMVTG 284
Cdd:cd05944 2 DDVAAYFHTGGTTGTPKLAQHTHSNEVYNawMLALNSLF---DPD---------DVLLCGLPLFHVNGSVVTLLTPLASG 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 285 NHNVLIT-----NPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRqlDFSALKVTNSGGTALVKATAERWESLTGC 359
Cdd:cd05944 70 AHVVLAGpagyrNPGLFDNFWKLVERYRITSLSTVPTVYAALLQVPVNA--DISSLRFAMSGAAPLPVELRARFEDATGL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 360 RIVEGYGLTETSPVASTNPYGELARLGTVGIPV--ADTAFKVIDDEGNEL---PLGERGELCIKGPQVMKGYWQQpEATA 434
Cdd:cd05944 148 PVVEGYGLTEATCLVAVNPPDGPKRPGSVGLRLpyARVRIKVLDGVGRLLrdcAPDEVGEICVAGPGVFGGYLYT-EGNK 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 435 QTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV-VAR 513
Cdd:cd05944 227 NAFVADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVqLKP 306
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 820875961 514 EGGLSVDELKAYCKANFTGY-KVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05944 307 GAVVEEEELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPALRADA 356
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
35-561 |
1.68e-67 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 227.79 E-value: 1.68e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 35 KRFADRPAFSN--LGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTER 112
Cdd:cd17642 28 ASVPGTIAFTDahTGVNYSYAEYLEMSVRLAEALKKY-GLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNER 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 113 EMRHQFKDAGARaLVYLNMfgKRVQEVLpdtgieylieaKMGDLLPMAKGWLINTVVDKVKKMVPAY-----HLPQAISF 187
Cdd:cd17642 107 ELDHSLNISKPT-IVFCSK--KGLQKVL-----------NVQKKLKIIKTIIILDSKEDYKGYQCLYtfitqNLPPGFNE 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 KqalregaalSHKPVTLSLD-DIAVLQYTGGTTGLAKGAMLTHGNLVANMlqvlacFSQHGPDGQKLIKEGQEVMIApLP 266
Cdd:cd17642 173 Y---------DFKPPSFDRDeQVALIMNSSGSTGLPKGVQLTHKNIVARF------SHARDPIFGNQIIPDTAILTV-IP 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 LYHIYAFTANcMCMMVTGNHNVLITNpRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALV 346
Cdd:cd17642 237 FHHGFGMFTT-LGYLICGFRVVLMYK-FEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDKYDLSNLHEIASGGAPLS 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 347 K----ATAERWEsLTGCRivEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVID-DEGNELPLGERGELCIKGPQ 421
Cdd:cd17642 315 KevgeAVAKRFK-LPGIR--QGYGLTETTSAILITPEGD-DKPGAVGKVVPFFYAKVVDlDTGKTLGPNERGELCVKGPM 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 422 VMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDER 501
Cdd:cd17642 391 IMKGYVNNPEATKALIDKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDED 470
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 502 SGEAVKLFVVAREG-GLSVDELKAYCKANFTGYK-VPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:cd17642 471 AGELPAAVVVLEAGkTMTEKEVMDYVASQVSTAKrLRGGVKFVDEVPKGLTGKIDRRKIREI 532
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
208-555 |
3.79e-67 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 220.99 E-value: 3.79e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAFTANcMCMMVTGNHN 287
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEAD----------VYLNMLPLFHIAGLNLA-LATFHAGGAN 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITnprdipgfikelgkwRFTALLGLN-------TLFV-------ALMDHPGFRQLDFSALKVTnsggTAL-VKATAER 352
Cdd:cd17637 70 VVME---------------KFDPAEALElieeekvTLMGsfppilsNLLDAAEKSGVDLSSLRHV----LGLdAPETIQR 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 353 WESLTGCRIVEGYGLTETSPVASTNPYGElaRLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEA 432
Cdd:cd17637 131 FEETTGATFWSLYGQTETSGLVTLSPYRE--RPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPEL 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 433 TAQTLDaDGWLKTGDIAVIDPDGFTRIVDRK--KDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV 510
Cdd:cd17637 209 TAYTFR-NGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVC 287
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 820875961 511 VAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd17637 288 VLKPGAtLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
49-560 |
6.31e-67 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 222.61 E-value: 6.31e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 49 TLTYGELDRLSAAFAAWLQQHTDlVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGAralvy 128
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGV-RKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDV----- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 129 lnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvtlSLDD 208
Cdd:cd05912 75 ----------------------------------------------------------------------------KLDD 78
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 209 IAVLQYTGGTTGLAKGAMLTHGNLVANmlqVLACFSQHGPDGQklikegqEVMIAPLPLYHIYAFTAncmcMMVTGNHNV 288
Cdd:cd05912 79 IATIMYTSGTTGKPKGVQQTFGNHWWS---AIGSALNLGLTED-------DNWLCALPLFHISGLSI----LMRSVIYGM 144
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 289 LIT-----NPRDIPGFIKElgkWRFTALLGLNTLFVALMD------HPGFRqldfsalKVTNSGGTA---LVKATAERwe 354
Cdd:cd05912 145 TVYlvdkfDAEQVLHLINS---GKVTIISVVPTMLQRLLEilgegyPNNLR-------CILLGGGPApkpLLEQCKEK-- 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 sltGCRIVEGYGLTET-SPVASTNPYGELARLGTVGIPVADTAFKVIDDEGnelPLGERGELCIKGPQVMKGYWQQPEAT 433
Cdd:cd05912 213 ---GIPVYQSYGMTETcSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQ---PPYEVGEILLKGPNVTKGYLNRPDAT 286
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 434 AQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAr 513
Cdd:cd05912 287 EESF-ENGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVS- 364
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 820875961 514 EGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05912 365 ERPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
35-560 |
1.41e-66 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 224.38 E-value: 1.41e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 35 KRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREM 114
Cdd:PRK06145 13 RRTPDRAALVYRDQEISYAEFHQRILQAAGMLHA-RGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 115 RHQFKDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvVDKVKKMVPAYHLPQAISFKQALREG 194
Cdd:PRK06145 92 AYILGDAGAKLLL-----------------------------------------VDEEFDAIVALETPKIVIDAAAQADS 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 AALS--HKPVT----LSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqKLIKEGqevmiaplPLY 268
Cdd:PRK06145 131 RRLAqgGLEIPpqaaVAPTDLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASE--RLLVVG--------PLY 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 269 HIYAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKA 348
Cdd:PRK06145 201 HVGAFDLPGIAVLWVGG-TLRIHREFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGGGEKTPES 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 349 TAERWESL-TGCRIVEGYGLTETSPVASTNPYG-ELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGY 426
Cdd:PRK06145 280 RIRDFTRVfTRARYIDAYGLTETCSGDTLMEAGrEIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGY 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:PRK06145 360 WKDPEKTAEAF-YGDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERI 438
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 507 KLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK06145 439 TAVVVLNPGAtLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLRD 493
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
32-560 |
4.25e-66 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 224.42 E-value: 4.25e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 32 RSCKRFADRPAF------SNLGKTLTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQYPIAVFGALRAGLIVVnt 105
Cdd:cd05931 1 RRAAARPDRPAYtflddeGGREETLTYAELDRRARAIAARLQAVGK--PGDRVLLLAPPGLDFVAAFLGCLYAGAIAV-- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 nPLYTEREMRHQ------FKDAGARAlvylnmfgkrvqeVLPDTGIEYLIEAKMGDLLPMAKGWLIntVVDkvkkmvpay 179
Cdd:cd05931 77 -PLPPPTPGRHAerlaaiLADAGPRV-------------VLTTAAALAAVRAFAASRPAAGTPRLL--VVD--------- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 180 hlpqaisfkQALREGAALSHkPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsQHGPDgqklikegqE 259
Cdd:cd05931 132 ---------LLPDTSAADWP-PPSPDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAY-GLDPG---------D 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 260 VMIAPLPLYHIYAFTANCMCMMVTGNHNVLITnPRDipgFIKELGKW-----RFTAllglnTLFVAlmdhPGF------- 327
Cdd:cd05931 192 VVVSWLPLYHDMGLIGGLLTPLYSGGPSVLMS-PAA---FLRRPLRWlrlisRYRA-----TISAA----PNFaydlcvr 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 328 -------RQLDFSALKVTNSGG--------TALVKATAE---RWESLTGCrivegYGLTE-----------TSPVASTNP 378
Cdd:cd05931 259 rvrdedlEGLDLSSWRVALNGAepvrpatlRRFAEAFAPfgfRPEAFRPS-----YGLAEatlfvsggppgTGPVVLRVD 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 379 YGELA--------------RLGTVGIPVADTAFKVIDDEGN-ELPLGERGELCIKGPQVMKGYWQQPEATAQT------L 437
Cdd:cd05931 334 RDALAgravavaaddpaarELVSCGRPLPDQEVRIVDPETGrELPDGEVGEIWVRGPSVASGYWGRPEATAETfgalaaT 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 438 DADGWLKTGDIAVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKV---ANCAAIGVPDERSGEAVklFVVARE 514
Cdd:cd05931 414 DEGGWLRTGDLGFLH-DGELYITGRLKDLIIVRGRNHYPQDIEATAEEAHPAlrpGCVAAFSVPDDGEERLV--VVAEVE 490
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|...
gi 820875961 515 GGLSVDELKAYCKANF----TGYKVPKHIVL---RESLPMTPVGKILRRELRD 560
Cdd:cd05931 491 RGADPADLAAIAAAIRaavaREHGVAPADVVlvrPGSIPRTSSGKIQRRACRA 543
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
38-559 |
4.31e-66 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 222.17 E-value: 4.31e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAFSNLGKTLTYGELDRLSAAFAAwlqqhtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQ 117
Cdd:PRK07787 14 DIADAVRIGGRVLSRSDLAGAATAVAE------RVAGARRVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHI 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 118 FKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDL-LPmakgwlintvvdkvkkmvpayHLPqaisfkqaLREGAA 196
Cdd:PRK07787 88 LADSGAQAW---------------------LGPAPDDPAgLP---------------------HVP--------VRLHAR 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 197 LSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmLQVLACFSQHGPDgqklikegqEVMIAPLPLYHIYAFTAN 276
Cdd:PRK07787 118 SWHRYPEPDPDAPALIVYTSGTTGPPKGVVLSRRAIAAD-LDALAEAWQWTAD---------DVLVHGLPLFHVHGLVLG 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 277 CMCMMVTGNHNVLITNPRdiPGFIKELGKWRFTALLGLNTLFVALMDHPGF-RQLDFSALKVtnSGGTALVKATAERWES 355
Cdd:PRK07787 188 VLGPLRIGNRFVHTGRPT--PEAYAQALSEGGTLYFGVPTVWSRIAADPEAaRALRGARLLV--SGSAALPVPVFDRLAA 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LTGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIDDEGNELPL-GER-GELCIKGPQVMKGYWQQPEAT 433
Cdd:PRK07787 264 LTGHRPVERYGMTETLITLSTRADGE-RRPGWVGLPLAGVETRLVDEDGGPVPHdGETvGELQVRGPTLFDGYLNRPDAT 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 434 AQTLDADGWLKTGDIAVIDPDGFTRIVDRKK-DMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVA 512
Cdd:PRK07787 343 AAAFTADGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVG 422
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 820875961 513 REgGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK07787 423 AD-DVAADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
39-561 |
2.91e-65 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 220.60 E-value: 2.91e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:PRK03640 17 DRTAIEFEEKKVTFMELHEAVVSVAGKLAALG-VKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVYLNMFGKRVQEVlpdtgieylIEAKMGDLLPMAKgwlintvvdkvkkmvpayhlpQAISFKQalregaals 198
Cdd:PRK03640 96 DDAEVKCLITDDDFEAKLIPG---------ISVKFAELMNGPK---------------------EEAEIQE--------- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hkpvTLSLDDIAVLQYTGGTTGLAKGAMLTHGN----LVANMLQVlacfsqhgpdGqklIKEgQEVMIAPLPLYHIYAFT 274
Cdd:PRK03640 137 ----EFDLDEVATIMYTSGTTGKPKGVIQTYGNhwwsAVGSALNL----------G---LTE-DDCWLAAVPIFHISGLS 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 275 AncmcMM---VTGNHNVLIT--NPRDIPGFIKElgkWRFTAL----LGLNTLFVALMD---HPGFRQLDFsalkvtnsGG 342
Cdd:PRK03640 199 I----LMrsvIYGMRVVLVEkfDAEKINKLLQT---GGVTIIsvvsTMLQRLLERLGEgtyPSSFRCMLL--------GG 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 343 TALVKATAERWEsLTGCRIVEGYGLTET-SPVASTNPYGELARLGTVGIPVADTAFKvIDDEGNELPLGERGELCIKGPQ 421
Cdd:PRK03640 264 GPAPKPLLEQCK-EKGIPVYQSYGMTETaSQIVTLSPEDALTKLGSAGKPLFPCELK-IEKDGVVVPPFEEGEIVVKGPN 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 422 VMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDER 501
Cdd:PRK03640 342 VTKGYLNREDATRETFQ-DGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDK 420
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 502 SGEAVKLFVVArEGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK03640 421 WGQVPVAFVVK-SGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
39-560 |
3.48e-65 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 221.10 E-value: 3.48e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAF--SNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRH 116
Cdd:PRK13391 12 DKPAVimASTGEVVTYRELDERSNRLAHLFRSL-GLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAY 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 117 QFKDAGARALVylnmfgkrvqevlpDTGIEYLIEAKMGDLLPMAKGWLintVVDKVKKMVPAYHLPQAIsfkqalregAA 196
Cdd:PRK13391 91 IVDDSGARALI--------------TSAAKLDVARALLKQCPGVRHRL---VLDGDGELEGFVGYAEAV---------AG 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 197 LSHKPvtlsLDDIAV---LQYTGGTTGLAKG--AMLTHGNLVANmLQVLACFsqhgpdgQKLIKEGQE-VMIAPLPLYHI 270
Cdd:PRK13391 145 LPATP----IADESLgtdMLYSSGTTGRPKGikRPLPEQPPDTP-LPLTAFL-------QRLWGFRSDmVYLSPAPLYHS 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 271 yAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHP-GFRQ-LDFSALKVTNSGGT---AL 345
Cdd:PRK13391 213 -APQRAVMLVIRLGG-TVIVMEHFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPeEVRDkYDLSSLEVAIHAAApcpPQ 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWeslTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTaFKVIDDEGNELPLGERGELCIKGPQVMKg 425
Cdd:PRK13391 291 VKEQMIDW---WGPIIHEYYAATEGLGFTACDSEEWLAHPGTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEGGRPFE- 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 426 YWQQPEATAQTLDADG-WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGE 504
Cdd:PRK13391 366 YLNDPAKTAEARHPDGtWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGE 445
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 505 AVKLFVVAREGGLSVD----ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK13391 446 EVKAVVQPVDGVDPGPalaaELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRD 505
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
39-552 |
1.22e-63 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 217.45 E-value: 1.22e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:PRK07798 18 DRVALVCGDRRLTYAELEERANRLAHYLIAQ-GLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELRYLL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVYLNMFGKRVQEVLPDT-GIEYLIEAKMGDLLPMakgwlintvvdkvkkmvpayhLPQAISFKQALREGAAl 197
Cdd:PRK07798 97 DDSDAVALVYEREFAPRVAEVLPRLpKLRTLVVVEDGSGNDL---------------------LPGAVDYEDALAAGSP- 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 198 SHKPVTLSLDDIAVLqYTGGTTGLAKGAMLTHGNL-VANMLQVLACFSQHGPDGQKLIKEGQE-----VMIAPlPLYHIY 271
Cdd:PRK07798 155 ERDFGERSPDDLYLL-YTGGTTGMPKGVMWRQEDIfRVLLGGRDFATGEPIEDEEELAKRAAAgpgmrRFPAP-PLMHGA 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 272 AFTANCMCMMvTGNHNVLITNPRDIPgfiKELgkWRFTALLGLNTLFV-----------ALmDHPGfrQLDFSALKVTNS 340
Cdd:PRK07798 233 GQWAAFAALF-SGQTVVLLPDVRFDA---DEV--WRTIEREKVNVITIvgdamarplldAL-EARG--PYDLSSLFAIAS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 341 GGTALVKATAERW-ESLTGCRIVEGYGLTETSPVAS-TNPYGELARLGTVGIPVADTafKVIDDEGNELPLG--ERGELC 416
Cdd:PRK07798 304 GGALFSPSVKEALlELLPNVVLTDSIGSSETGFGGSgTVAKGAVHTGGPRFTIGPRT--VVLDEDGNPVEPGsgEIGWIA 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPqVMKGYWQQPEATAQTL-DADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:PRK07798 382 RRGH-IPLGYYKDPEKTAETFpTIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADAL 460
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 494 AIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGK 552
Cdd:PRK07798 461 VVGVPDERWGQEVVAVVQLREGArPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
57-559 |
2.83e-63 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 214.61 E-value: 2.83e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 57 RLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQYPIAVFGALRAG----LIVVNTNPLYTEREMRHQFKDAGARAlvylnmf 132
Cdd:cd05922 2 GVSAAASALLEAGGV--RGERVVLILPNRFTYIELSFAVAYAGgrlgLVFVPLNPTLKESVLRYLVADAGGRI------- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 133 gkrvqeVLPDTGIeylieakmgdllpmakgwlintvVDKVKKMVPAYHLPQAISFKQALReGAALSHKPVTLSLDDIAVL 212
Cdd:cd05922 73 ------VLADAGA-----------------------ADRLRDALPASPDPGTVLDADGIR-AARASAPAHEVSHEDLALL 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 213 QYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAFTancmcmmVTGNHNV---- 288
Cdd:cd05922 123 LYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADD----------RALTVLPLSYDYGLS-------VLNTHLLrgat 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 289 LITNPRDIP--GFIKELGKWRFTALLGLNTLFvALMDHPGFRQLDFSALK-VTNSGGtALVKATAERW-ESLTGCRIVEG 364
Cdd:cd05922 186 LVLTNDGVLddAFWEDLREHGATGLAGVPSTY-AMLTRLGFDPAKLPSLRyLTQAGG-RLPQETIARLrELLPGAQVYVM 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 365 YGLTETSPVASTNPYGELA-RLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWL 443
Cdd:cd05922 264 YGQTEATRRMTYLPPERILeKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVL 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 444 KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDErSGEAVKLFVVAREgGLSVDELK 523
Cdd:cd05922 344 HTGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDP-LGEKLALFVTAPD-KIDPKDVL 421
|
490 500 510
....*....|....*....|....*....|....*.
gi 820875961 524 AYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05922 422 RSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
32-558 |
7.06e-63 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 214.11 E-value: 7.06e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 32 RSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTE 111
Cdd:cd05920 23 RSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRG-LGIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPSHRR 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 112 REMRHQFKDAGARALVylnmfGKRVQEvlpdtgieylieakmgdllpmakgwlintvvdkvkkmvPAYHLPQAisfkqal 191
Cdd:cd05920 102 SELSAFCAHAEAVAYI-----VPDRHA--------------------------------------GFDHRALA------- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 192 REGAAlshkpvtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANM-LQVLAC-FSQHgpdgqklikegqEVMIAPLPLYH 269
Cdd:cd05920 132 RELAE--------SIPEVALFLLSGGTTGTPKLIPRTHNDYAYNVrASAEVCgLDQD------------TVYLAVLPAAH 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 IYAFTANCMC-MMVTGNHNVLITNPRDIPGFiKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKA 348
Cdd:cd05920 192 NFPLACPGVLgTLLAGGRVVLAPDPSPDAAF-PLIEREGVTVTALVPALVSLWLDAAASRRADLSSLRLLQVGGARLSPA 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 349 TAERWESLTGCRIVEGYGLTEtspvastnpyGEL--ARL--------GTVGIPV-ADTAFKVIDDEGNELPLGERGELCI 417
Cdd:cd05920 271 LARRVPPVLGCTLQQVFGMAE----------GLLnyTRLddpdeviiHTQGRPMsPDDEIRVVDEEGNPVPPGEEGELLT 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 418 KGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGV 497
Cdd:cd05920 341 RGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAM 420
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 498 PDERSGEAVKLFVVAREGGLSVDELKAYCK-ANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05920 421 PDELLGERSCAFVVLRDPPPSAAQLRRFLReRGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
28-562 |
2.12e-62 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 222.49 E-value: 2.12e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSN-LGKTLTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTN 106
Cdd:PRK08633 619 EAWIDTAKRNWSRLAVADsTGGELSYGKALTGALALARLLKRELK--DEENVGILLPPSVAGALANLALLLAGKVPVNLN 696
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 107 plYT--EREMRHQFKDAGARALVYLNMF------GKRVQEVLPDTGIEYL--IEAKMGDllpmakgwlintvVDKVKKMV 176
Cdd:PRK08633 697 --YTasEAALKSAIEQAQIKTVITSRKFleklknKGFDLELPENVKVIYLedLKAKISK-------------VDKLTALL 761
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 177 PAYHLPQAIsFKQALREGAalshkpvtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQV--LACFSQHgpdgqkli 254
Cdd:PRK08633 762 AARLLPARL-LKRLYGPTF---------KPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQIsdVFNLRND-------- 823
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 255 kegqEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSA 334
Cdd:PRK08633 824 ----DVILSSLPFFHSFGLTVTLWLPLLEGIKVVYHPDPTDALGIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMFAS 899
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 335 LKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELA---------RLGTVGIPVADTAFKVID-DEG 404
Cdd:PRK08633 900 LRLVVAGAEKLKPEVADAFEEKFGIRILEGYGATETSPVASVNLPDVLAadfkrqtgsKEGSVGMPLPGVAVRIVDpETF 979
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 405 NELPLGERGELCIKGPQVMKGYWQQPEATAQ---TLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIED 481
Cdd:PRK08633 980 EELPPGEDGLILIGGPQVMKGYLGDPEKTAEvikDIDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEE 1059
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 482 ---VIMGHPKVAnCAAIGVPDERSGEavKLFVVAREGGLSVDELKAYCKANF-TGYKVPKHIVLRESLPMTPVGKILRRE 557
Cdd:PRK08633 1060 elaKALGGEEVV-FAVTAVPDEKKGE--KLVVLHTCGAEDVEELKRAIKESGlPNLWKPSRYFKVEALPLLGSGKLDLKG 1136
|
....*
gi 820875961 558 LRDIA 562
Cdd:PRK08633 1137 LKELA 1141
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
48-560 |
3.14e-61 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 208.44 E-value: 3.14e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALV 127
Cdd:cd05971 5 EKVTFKELKTASNRFANVLKE-IGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 ylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvTLSLD 207
Cdd:cd05971 84 ---------------------------------------------------------------------------TDGSD 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGQKL--------IKEGQEVMIA------PLPLYHIYAF 273
Cdd:cd05971 89 DPALIIYTSGTTGPPKGALHAHRVLLGHLPGVQFPFNLFPRDGDLYwtpadwawIGGLLDVLLPslyfgvPVLAHRMTKF 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 274 TANCMCMMVTgNHNVliTN---PRDIPGFIKELGKWRftallglntlfvalmDHPGFRqldfsaLKVTNSGGTAL-VKAT 349
Cdd:cd05971 169 DPKAALDLMS-RYGV--TTaflPPTALKMMRQQGEQL---------------KHAQVK------LRAIATGGESLgEELL 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 350 AERWESLtGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQ--VMKGYW 427
Cdd:cd05971 225 GWAREQF-GVEVNEFYGQTECNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDpvAFLGYW 303
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 428 QQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVK 507
Cdd:cd05971 304 NNPSATEKKM-AGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVK 382
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 508 LFVVAREGGLSVDELKA----YCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05971 383 AFVVLNPGETPSDALAReiqeLVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
47-560 |
3.49e-61 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 210.33 E-value: 3.49e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARAL 126
Cdd:PRK12406 9 DRRRSFDELAQRAARAAGGLAA-LGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VYLNMFGKRVQEVLPdTGIEYLieakmgdllpmakgwlintVVDKVKKMVPAYHLPQAI----SFKQALREGAALSHKPV 202
Cdd:PRK12406 88 IAHADLLHGLASALP-AGVTVL-------------------SVPTPPEIAAAYRISPALltppAGAIDWEGWLAQQEPYD 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 203 TLSLDDIAVLQYTGGTTGLAKG---AMLTHGNLVANMLQVLACFSQhgpdgqkliKEGQEVMIaPLPLYHiyafTA-NCM 278
Cdd:PRK12406 148 GPPVPQPQSMIYTSGTTGHPKGvrrAAPTPEQAAAAEQMRALIYGL---------KPGIRALL-TGPLYH----SApNAY 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLITNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHPGF--RQLDFSALK-VTNSGGT--ALVK-ATAE 351
Cdd:PRK12406 214 GLRAGRLGGVLVLQPRfDPEELLQLIERHRITHMHMVPTMFIRLLKLPEEvrAKYDVSSLRhVIHAAAPcpADVKrAMIE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 352 RWesltGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMK-GYWQQP 430
Cdd:PRK12406 294 WW----GPVIYEYYGSTESGAVTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKP 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 431 EATAQtLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV 510
Cdd:PRK12406 370 EKRAE-IDRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVV 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 820875961 511 VAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK12406 449 EPQPGAtLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRD 499
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
207-555 |
8.06e-61 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 208.37 E-value: 8.06e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQvLACFSQHGPdGQKlikegqevMIAPLPLYHIY-------AFTANCMC 279
Cdd:cd17640 88 DDLATIIYTSGTTGNPKGVMLTHANLLHQIRS-LSDIVPPQP-GDR--------FLSILPIWHSYersaeyfIFACGCSQ 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 280 MMVTgnhnvlitnprdIPGFIKELGKWRFTALLGLNTLFVAL--------MDHPGFRQLDFSALKVTN------SGGTAL 345
Cdd:cd17640 158 AYTS------------IRTLKDDLKRVKPHYIVSVPRLWESLysgiqkqvSKSSPIKQFLFLFFLSGGifkfgiSGGGAL 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWESlTGCRIVEGYGLTETSPVASTNPYGELARlGTVGIPVADTAFKVIDDEGNE-LPLGERGELCIKGPQVMK 424
Cdd:cd17640 226 PPHVDTFFEA-IGIEVLNGYGLTETSPVVSARRLKCNVR-GSVGRPLPGTEIKIVDPEGNVvLPPGEKGIVWVRGPQVMK 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 425 GYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANCAAIG------- 496
Cdd:cd17640 304 GYYKNPEATSKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVVGqdqkrlg 383
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 497 ---VPD----ERSGEAVKLFVVAREGGL--SVDELKAYCK------ANFTGYK-----VPKHIVLRESLP---MTPVGKI 553
Cdd:cd17640 384 aliVPNfeelEKWAKESGVKLANDRSQLlaSKKVLKLYKNeikdeiSNRPGFKsfeqiAPFALLEEPFIEngeMTQTMKI 463
|
..
gi 820875961 554 LR 555
Cdd:cd17640 464 KR 465
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
38-558 |
3.14e-60 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 205.84 E-value: 3.14e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQ 117
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRER-GVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 118 FKDAGARALVYlnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaal 197
Cdd:cd05930 80 LEDSGAKLVLT--------------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 198 shkpvtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVaNMLQVLACFSQHGPDGQKLikegqevMIAPlplyhiYAFTA-- 275
Cdd:cd05930 91 -------DPDDLAYVIYTSGSTGKPKGVMVEHRGLV-NLLLWMQEAYPLTPGDRVL-------QFTS------FSFDVsv 149
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 276 NCMCMMVTGNHNVLITNP---RDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRqlDFSALKVTNSGGTALVKATAER 352
Cdd:cd05930 150 WEIFGALLAGATLVVLPEevrKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA--ALPSLRLVLVGGEALPPDLVRR 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 353 W-ESLTGCRIVEGYGLTETSpVAST----NPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYW 427
Cdd:cd05930 228 WrELLPGARLVNLYGPTEAT-VDATyyrvPPDDEEDGRVPIGRPIPNTRVYVLDENLRPVPPGVPGELYIGGAGLARGYL 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 428 QQPEATAQTLDADGWL------KTGDIAVIDPDGftRIV--DRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPD 499
Cdd:cd05930 307 NRPELTAERFVPNPFGpgermyRTGDLVRWLPDG--NLEflGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVARED 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 500 ERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05930 385 GDGEKRLVAYVVPDEGGeLDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
36-561 |
2.37e-59 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 206.32 E-value: 2.37e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 36 RFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPN--VLQYPIAVFGALRAGLIVVNTNplyterE 113
Cdd:PRK07788 61 RAPDRAALIDERGTLTYAELDEQSNALARGLLAL-GVRAGDGVAVLARNhrGFVLALYAAGKVGARIILLNTG------F 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 114 MRHQFKDA----GARALVYLNMFGKRVQEVLPDtgieylieakMGDLLpmakgwlinTVVDKVKKMVPAyhLPQAISFkQ 189
Cdd:PRK07788 134 SGPQLAEVaareGVKALVYDDEFTDLLSALPPD----------LGRLR---------AWGGNPDDDEPS--GSTDETL-D 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 190 ALREGAALSHKPVTLSLDDIAVLqyTGGTTGLAKGAMLTHgnlvANMLQVLACFSQHGPDGQKlikegqEVMIAPLPLYH 269
Cdd:PRK07788 192 DLIAGSSTAPLPKPPKPGGIVIL--TSGTTGTPKGAPRPE----PSPLAPLAGLLSRVPFRAG------ETTLLPAPMFH 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 IYAFtANCMCMMVTGNHNVLitnPR--DIPGFIKELGKWRFTALLGLNTLFVALMDHPG--FRQLDFSALKVTNSGGTAL 345
Cdd:PRK07788 260 ATGW-AHLTLAMALGSTVVL---RRrfDPEATLEDIAKHKATALVVVPVMLSRILDLGPevLAKYDTSSLKIIFVSGSAL 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERWESLTGCRIVEGYGLTETSPVASTNPyGELARL-GTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMK 424
Cdd:PRK07788 336 SPELATRALEAFGPVLYNLYGSTEVAFATIATP-EDLAEApGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFE 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 425 GYWQQPeaTAQTldADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGE 504
Cdd:PRK07788 415 GYTDGR--DKQI--IDGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQ 490
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 505 AVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK07788 491 RLRAFVVKAPGaALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
25-558 |
2.75e-59 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 204.67 E-value: 2.75e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPAFSNL--GKTLTYGEL-DRLSAAFAAWLQQhtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI 101
Cdd:cd05923 2 TVFEMLRRAASRAPDACAIADParGLRLTYSELrARIEAVAARLHAR--GLRPGQRVAVVLPNSVEAVIALLALHRLGAV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 102 VVNTNPLYTEREMRHQFKDAGARALVYLNmfGKRVQEVLPDTGIEYLieakmgdllpmakgwlinTVVDKVKKMVPAYHL 181
Cdd:cd05923 80 PALINPRLKAAELAELIERGEMTAAVIAV--DAQVMDAIFQSGVRVL------------------ALSDLVGLGEPESAG 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 182 PqAISFKQALREGAALSHkpvtlslddiavlqYTGGTTGLAKGAMLTHGnlvANMLQVLACFSQHGpdgqkLIKEGQEVM 261
Cdd:cd05923 140 P-LIEDPPREPEQPAFVF--------------YTSGTTGLPKGAVIPQR---AAESRVLFMSTQAG-----LRHGRHNVV 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 262 IAPLPLYHIYAFTANCMCMMVTGNHNVLIT--NPRDIPGFIKELgkwRFTALLGLNTLFVALMDHPGFRQLDFSALKVTN 339
Cdd:cd05923 197 LGLMPLYHVIGFFAVLVAALALDGTYVVVEefDPADALKLIEQE---RVTSLFATPTHLDALAAAAEFAGLKLSSLRHVT 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 340 SGGTALVKATAERWESLTGCRIVEGYGLTEtspvASTNPYGELARLGTVGIPVADTAFKVIDDEGNE---LPLGERGELC 416
Cdd:cd05923 274 FAGATMPDAVLERVNQHLPGEKVNIYGTTE----AMNSLYMRDARTGTEMRPGFFSEVRIVRIGGSPdeaLANGEEGELI 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IK--GPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:cd05923 350 VAaaADAAFTGYLNQPEATAKKL-QDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVV 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 495 IGVPDERSGEAVKLFVVAREGGLSVDELKAYCKAN-FTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05923 429 IGVADERWGQSVTACVVPREGTLSADELDQFCRASeLADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
31-560 |
7.94e-58 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 200.60 E-value: 7.94e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 31 ERSCKRFADRPAFSNLGKTLTYGELD----RLSAAFAAWLQQhtdlvPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTN 106
Cdd:cd12118 11 ERAAAVYPDRTSIVYGDRRYTWRQTYdrcrRLASALAALGIS-----RGDTVAVLAPNTPAMYELHFGVPMAGAVLNALN 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 107 PLYTEREMRHQFKDAGARALvylnmfgkrvqevLPDTGIEYlieakmGDLLPMAKGWlintvvdkvkkmvpayhlPQAIs 186
Cdd:cd12118 86 TRLDAEEIAFILRHSEAKVL-------------FVDREFEY------EDLLAEGDPD------------------FEWI- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 187 fkqalregaalshKPVtlSLDDIAVLQYTGGTTGLAKGAMLTH-GNLVANMLQVLACFSQHGPdgqklikegqeVMIAPL 265
Cdd:cd12118 128 -------------PPA--DEWDPIALNYTSGTTGRPKGVVYHHrGAYLNALANILEWEMKQHP-----------VYLWTL 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 266 PLYHiyaftANCMC----MMVTGNHNVLITNPrDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSG 341
Cdd:cd12118 182 PMFH-----CNGWCfpwtVAAVGGTNVCLRKV-DAKAIYDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPHRVHVMTA 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTALVKATAERWESLtGCRIVEGYGLTETSPVASTN---------PYGELARL-GTVGIP-VADTAFKVIDDEGNE-LPL 409
Cdd:cd12118 256 GAPPPAAVLAKMEEL-GFDVTHVYGLTETYGPATVCawkpewdelPTEERARLkARQGVRyVGLEEVDVLDPETMKpVPR 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 410 -GER-GELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHP 487
Cdd:cd12118 335 dGKTiGEIVFRGNIVMKGYLKNPEATAEAF-RGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHP 413
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 488 KVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLREsLPMTPVGKILRRELRD 560
Cdd:cd12118 414 AVLEAAVVARPDEKWGEVPCAFVELKEGAkVTEEEIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVLRD 486
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
47-555 |
3.30e-57 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 198.44 E-value: 3.30e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARAL 126
Cdd:cd05914 5 GEPLTYKDLADNIAKFALLLK-INGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintVVDKvkkmvpayhlpqaisfkqalregaalshkpvtlsl 206
Cdd:cd05914 84 F----------------------------------------VSDE----------------------------------- 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACfsqhgpdgqKLIKEGqEVMIAPLPLYHIYAFTANCMCMMVTGNH 286
Cdd:cd05914 89 DDVALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEV---------VLLGKG-DKILSILPLHHIYPLTFTLLLPLLNGAH 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLITNPRdiPGFIKELGKWRFTALLGLNTLFV--------------------ALMDHP---GFRQLDFSAL-------- 335
Cdd:cd05914 159 VVFLDKIP--SAKIIALAFAQVTPTLGVPVPLViekifkmdiipkltlkkfkfKLAKKInnrKIRKLAFKKVheafggni 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 336 KVTNSGGTALVKATAERWESLtGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAFKvIDDEGnelPLGERGEL 415
Cdd:cd05914 237 KEFVIGGAKINPDVEEFLRTI-GFPYTIGYGMTETAPIISYSPPNRI-RLGSAGKVIDGVEVR-IDSPD---PATGEGEI 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 416 CIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIV-SGFNVYPNEIEDVIMGHPKVANcAA 494
Cdd:cd05914 311 IVRGPNVMKGYYKNPEATAEAFDKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLsSGKNIYPEEIEAKINNMPFVLE-SL 389
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 495 IGVPDERS-GEAVKLFVVAREGGLSVDELKAYCK-----------ANFTgyKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd05914 390 VVVQEKKLvALAYIDPDFLDVKALKQRNIIDAIKwevrdkvnqkvPNYK--KISKVKIVKEEFEKTPKGKIKR 460
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
35-558 |
1.38e-55 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 193.62 E-value: 1.38e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 35 KRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLyterem 114
Cdd:cd05945 2 AANPDRPAVVEGGRTLTYRELKERADALAAALA-SLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYV---PL------ 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 115 rhqfkDAGARAlvylnmfgKRVQEVLPDTGieylieakmgdllPMAkgwLINTVvdkvkkmvpayhlpqaisfkqalreg 194
Cdd:cd05945 72 -----DASSPA--------ERIREILDAAK-------------PAL---LIADG-------------------------- 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 aalshkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsqhgpdgqkLIKEGQEVM-IAP----LPLYH 269
Cdd:cd05945 97 ------------DDNAYIIFTSGSTGRPKGVQISHDNLVSFTNWMLSDF---------PLGPGDVFLnQAPfsfdLSVMD 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 IYaftancmCMMVTGNhnVLITNPRDIPGFIKELGkwRFTALLGLNTL-----FVA-LMDHPGFRQLDFSALKVTNSGGT 343
Cdd:cd05945 156 LY-------PALASGA--TLVPVPRDATADPKQLF--RFLAEHGITVWvstpsFAAmCLLSPTFTPESLPSLRHFLFCGE 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 344 ALVKATAERW-ESLTGCRIVEGYGLTETSpVAST------NPYGELARLgTVGIPVADTAFKVIDDEGNELPLGERGELC 416
Cdd:cd05945 225 VLPHKTARALqQRFPDARIYNTYGPTEAT-VAVTyievtpEVLDGYDRL-PIGYAKPGAKLVILDEDGRPVPPGEKGELV 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLDAD---GWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd05945 303 ISGPSVSKGYLNNPEKTAAAFFPDegqRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAV 382
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 494 AIGVPDERSGEAVKLFVVAREGGLSVD--ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd05945 383 VVPKYKGEKVTELIAFVVPKPGAEAGLtkAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
54-560 |
3.70e-53 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 189.21 E-value: 3.70e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 54 ELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYLNMFG 133
Cdd:cd05928 46 ELGSLSRKAANVLSGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELA 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 134 KRVQEVL---PDTGIEYLIEAKMGDllpmakGWLintvvdkvkkmvpayhlpqaiSFKQALREgAALSHKPVTLSLDDIA 210
Cdd:cd05928 126 PEVDSVAsecPSLKTKLLVSEKSRD------GWL---------------------NFKELLNE-ASTEHHCVETGSQEPM 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 211 VLQYTGGTTGLAKGAMLTHGNLvanmlqvlacfsQHG--PDGQKLIK-EGQEVMI-------APLPLYHIYAFTANCMCM 280
Cdd:cd05928 178 AIYFTSGTTGSPKMAEHSHSSL------------GLGlkVNGRYWLDlTASDIMWntsdtgwIKSAWSSLFEPWIQGACV 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 281 MVtgnHNVlitnPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHpGFRQLDFSALKVTNSGGTALVKATAERWESLTGC 359
Cdd:cd05928 246 FV---HHL----PRfDPLVILKTLSSYPITTFCGAPTVYRMLVQQ-DLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGL 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 360 RIVEGYGLTETSPVAStNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIK-GPQ----VMKGYWQQPEATA 434
Cdd:cd05928 318 DIYEGYGQTETGLICA-NFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRvKPIrpfgLFSGYVDNPEKTA 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 435 QTLDADGWLkTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVARE 514
Cdd:cd05928 397 ATIRGDFYL-TGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFVVLAP 475
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 820875961 515 GGLSVD------ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05928 476 QFLSHDpeqltkELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
201-560 |
4.39e-53 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 187.58 E-value: 4.39e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 201 PVTLSLDDIA--VLQYTGGTTGLAKGAMLTH-GNLVANMLQVLACF-SQHGPDgqklikegqEVMIAPLPLYHIYAFTAn 276
Cdd:cd05929 117 PETPIEDEAAgwKMLYSGGTTGRPKGIKRGLpGGPPDNDTLMAAALgFGPGAD---------SVYLSPAPLYHAAPFRW- 186
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 277 CMCMMVTGNHNVLITNpRDIPGFIKELGKWRFTALLGLNTLFVALMDHPG--FRQLDFSALKVTNSGGTALVKATAERWE 354
Cdd:cd05929 187 SMTALFMGGTLVLMEK-FDPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEavRNAYDLSSLKRVIHAAAPCPPWVKEQWI 265
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 SLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVAdTAFKVIDDEGNELPLGERGELCIKGPQVmKGYWQQPEATA 434
Cdd:cd05929 266 DWGGPIIWEYYGGTEGQGLTIINGEEWLTHPGSVGRAVL-GKVHILDEDGNEVPPGEIGEVYFANGPG-FEYTNDPEKTA 343
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 435 QTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV---- 510
Cdd:cd05929 344 AARNEGGWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVqpap 423
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 820875961 511 VAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05929 424 GADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
25-556 |
5.27e-53 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 188.94 E-value: 5.27e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPA--FSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:PRK05852 17 RIADLVEVAATRLPEAPAlvVTADRIAISYRDLARLVDDLAGQLTR-SGLLPGDRVALRMGSNAEFVVALLAASRADLVV 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARAlvylnmfgkrvqeVLPDTgieyliEAKMGDLLPMAKGWLINTVVDKVKKMVPAYHLP 182
Cdd:PRK05852 96 VPLDPALPIAEQRVRSQAAGARV-------------VLIDA------DGPHDRAEPTTRWWPLTVNVGGDSGPSGGTLSV 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 QaisfKQALREGAALSHKPVTLSLDDiAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsQHGPdgqklikegQEVMI 262
Cdd:PRK05852 157 H----LDAATEPTPATSTPEGLRPDD-AMIMFTGGTTGLPKMVPWTHANIASSVRAIITGY-RLSP---------RDATV 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHIYAFTANCMCMMVTGNHNVLITNPRdipgF--------IKELGKWRFTALLGLNTLFVALMDHPGFRQLDfSA 334
Cdd:PRK05852 222 AVMPLYHGHGLIAALLATLASGGAVLLPARGR----FsahtfwddIKAVGATWYTAVPTIHQILLERAATEPSGRKP-AA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 335 LKVTNSGGTALVKATAERWESLTGCRIVEGYGLTE-TSPVASTNPYG----ELARLgTVGIPVADTA--FKVIDDEGNEL 407
Cdd:PRK05852 297 LRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEaTHQVTTTQIEGigqtENPVV-STGLVGRSTGaqIRIVGSDGLPL 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 408 PLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHP 487
Cdd:PRK05852 376 PAGAVGEVWLRGTTVVRGYLGDPTITAANF-TDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHP 454
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 488 KVANCAAIGVPDERSGEAVKLFVVARE-GGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRR 556
Cdd:PRK05852 455 NVMEAAVFGVPDQLYGEAVAAVIVPREsAPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
185-560 |
1.52e-52 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 187.81 E-value: 1.52e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 185 ISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqVLACFSQHGpdgqKLIKEGQE-VMIA 263
Cdd:cd05927 92 YSLEEFEKLGKKNKVPPPPPKPEDLATICYTSGTTGNPKGVMLTHGNIVSN---VAGVFKILE----ILNKINPTdVYIS 164
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 264 PLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPGFIKELgkwRFTALLG----LNTLFVALMD---------------- 323
Cdd:cd05927 165 YLPLAHIFERVVEALFLYHGAKIGFYSGDIRLLLDDIKAL---KPTVFPGvprvLNRIYDKIFNkvqakgplkrklfnfa 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 324 ---------------HPGFRQLDFSALK--------VTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYG 380
Cdd:cd05927 242 lnyklaelrsgvvraSPFWDKLVFNKIKqalggnvrLMLTGSAPLSPEVLEFLRVALGCPVLEGYGQTECTAGATLTLPG 321
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 381 ELaRLGTVGIPVADTAFKVID-DEGNELPLGE--RGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFT 457
Cdd:cd05927 322 DT-SVGHVGGPLPCAEVKLVDvPEMNYDAKDPnpRGEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWLPNGTL 400
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 458 RIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANC-----------AAIGVPDErsgEAVKLFVVAREGG--------- 516
Cdd:cd05927 401 KIIDRKKNIFKLSqGEYVAPEKIENIYARSPFVAQIfvygdslksflVAIVVPDP---DVLKEWAASKGGGtgsfeelck 477
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 517 ------LSVDELKAYCKAN-FTGYKVPKHIVLrESLP-------MTPVGKILRRELRD 560
Cdd:cd05927 478 npevkkAILEDLVRLGKENgLKGFEQVKAIHL-EPEPfsvenglLTPTFKLKRPQLKK 534
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
214-551 |
1.74e-52 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 182.12 E-value: 1.74e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 214 YTGGTTGLAKGAMLTHGNLVANMLQVLacfsqhgpDGQKLikEGQEVMIAPLPLYHIyAFTANCMCMMVTGNHNVLI--T 291
Cdd:cd17636 7 YTAAFSGRPNGALLSHQALLAQALVLA--------VLQAI--DEGTVFLNSGPLFHI-GTLMFTLATFHAGGTNVFVrrV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 292 NPRDIPGFI-KELGKWRFtallglntLFVALMDH----PGFRQLDFSALKVTNS--GGTALVKATAERWesltgCRIVEG 364
Cdd:cd17636 76 DAEEVLELIeAERCTHAF--------LLPPTIDQivelNADGLYDLSSLRSSPAapEWNDMATVDTSPW-----GRKPGG 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 365 YGLTETSPVASTNPYGELArLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLK 444
Cdd:cd17636 143 YGQTEVMGLATFAALGGGA-IGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRT-RGGWHH 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 445 TGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELK 523
Cdd:cd17636 221 TNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGaSVTEAELI 300
|
330 340
....*....|....*....|....*...
gi 820875961 524 AYCKANFTGYKVPKHIVLRESLPMTPVG 551
Cdd:cd17636 301 EHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
51-558 |
1.83e-52 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 184.96 E-value: 1.83e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGaralvyln 130
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQG-IRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLD-------- 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 131 mfgkrVQEVLPDTgieyLIEAKmgDLlpmakgwlintvvdkvkkmvpayhlpQAISFKQALREGAALSHKPVTLSLDDIA 210
Cdd:TIGR01923 72 -----VQLLLTDS----LLEEK--DF--------------------------QADSLDRIEAAGRYETSLSASFNMDQIA 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 211 VLQYTGGTTGLAKGAMLTHGNLVANMLQVLacfsqhgpdgQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVtGNHNVLI 290
Cdd:TIGR01923 115 TLMFTSGTTGKPKAVPHTFRNHYASAVGSK----------ENLGFTEDDNWLLSLPLYHISGLSILFRWLIE-GATLRIV 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 291 TNPRDIPGFIKelgKWRFTALLGLNTLFVALMDHPGFRQLdfsaLKVTNSGGTA----LVKATAERwesltGCRIVEGYG 366
Cdd:TIGR01923 184 DKFNQLLEMIA---NERVTHISLVPTQLNRLLDEGGHNEN----LRKILLGGSAipapLIEEAQQY-----GLPIYLSYG 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 367 LTET-SPVASTNPYGELARLGtVGIPVADTAFKVIDDEgnelpLGERGELCIKGPQVMKGYWQQPEATaQTLDADGWLKT 445
Cdd:TIGR01923 252 MTETcSQVTTATPEMLHARPD-VGRPLAGREIKIKVDN-----KEGHGEIMVKGANLMKGYLYQGELT-PAFEQQGWFNT 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 446 GDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVArEGGLSVDELKAY 525
Cdd:TIGR01923 325 GDIGELDGEGFLYVLGRRDDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVS-ESDISQAKLIAY 403
|
490 500 510
....*....|....*....|....*....|...
gi 820875961 526 CKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:TIGR01923 404 LTEKLAKYKVPIAFEKLDELPYNASGKILRNQL 436
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
48-560 |
7.03e-52 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 185.78 E-value: 7.03e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALV 127
Cdd:cd05970 46 RIFTFAELADYSDKTANFFKAM-GIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIV 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 YLNMFG--KRVQEVLPDTGIEYLIeAKMGDLLPmaKGWLinTVVDKVKKMVPAYHLPQAisfkqalregaalshkPVTLS 205
Cdd:cd05970 125 AIAEDNipEEIEKAAPECPSKPKL-VWVGDPVP--EGWI--DFRKLIKNASPDFERPTA----------------NSYPC 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 206 LDDIAVLQYTGGTTGLAKgaMLTHGNLVANMLQVLACFSQH-GPDGQKL-IKE------------GQEVMIAPLPLYHIY 271
Cdd:cd05970 184 GEDILLVYFSSGTTGMPK--MVEHDFTYPLGHIVTAKYWQNvREGGLHLtVADtgwgkavwgkiyGQWIAGAAVFVYDYD 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 272 AFTANCMcmmvtgnhnvlitnprdipgfIKELGKWRFTALLGLNTLFVALMdHPGFRQLDFSALKVTNSGGTALVKATAE 351
Cdd:cd05970 262 KFDPKAL---------------------LEKLSKYGVTTFCAPPTIYRFLI-REDLSRYDLSSLRYCTTAGEALNPEVFN 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 352 RWESLTGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIDDEGNELPLGERGELCI---KGPQV--MKGY 426
Cdd:cd05970 320 TFKEKTGIKLMEGFGQTETTLTIATFPWME-PKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIrtsKGKPVglFGGY 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:cd05970 399 YKDAEKTAEVWH-DGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVV 477
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 507 KLFVVAREGGLSVDELKA----YCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05970 478 KATIVLAKGYEPSEELKKelqdHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIRE 535
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
28-558 |
1.50e-50 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 180.86 E-value: 1.50e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:cd12117 1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAA-GVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALVYLNMFGKRVQEvLPDTGIEYlieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisf 187
Cdd:cd12117 80 ELPAERLAFMLADAGAKVLLTDRSLAGRAGG-LEVAVVID---------------------------------------- 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 kQALREGAALShKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVAnmlqvLACFSQHGPDGqklikeGQEVMI--APL 265
Cdd:cd12117 119 -EALDAGPAGN-PAVPVSPDDLAYVMYTSGSTGRPKGVAVTHRGVVR-----LVKNTNYVTLG------PDDRVLqtSPL 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 266 plyhiyAFTANCMCM---MVTGNHNVLItnPRDIPGFIKELGkwRFTALLGLNTLFV--ALmdhpgFRQLD------FSA 334
Cdd:cd12117 186 ------AFDASTFEIwgaLLNGARLVLA--PKGTLLDPDALG--ALIAEEGVTVLWLtaAL-----FNQLAdedpecFAG 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 335 LKVTNSGGTAL----VKATAERWESLtgcRIVEGYGLTETSPVASTNPYGELARLGT---VGIPVADTAFKVIDDEGNEL 407
Cdd:cd12117 251 LRELLTGGEVVspphVRRVLAACPGL---RLVNGYGPTENTTFTTSHVVTELDEVAGsipIGRPIANTRVYVLDEDGRPV 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 408 PLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIED 481
Cdd:cd12117 328 PPGVPGELYVGGDGLALGYLNRPALTAERFVADPFGpgerlyRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEA 407
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 482 VIMGHPKVANCAAIGVPDERSGEAVKLFVVArEGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd12117 408 ALRAHPGVREAVVVVREDAGGDKRLVAYVVA-EGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
51-494 |
1.62e-49 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 176.30 E-value: 1.62e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVyln 130
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLL--- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 131 mfgkrvqevlpdTGIEYLIEAKMgdllpmakgwlintvvdkvkkmVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIA 210
Cdd:TIGR01733 78 ------------TDSALASRLAG----------------------LVLPVILLDPLELAALDDAPAPPPPDAPSGPDDLA 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 211 VLQYTGGTTGLAKGAMLTHGNLVaNMLQVLAcfsqhgpdgQKLIKEGQEVMIAplplYHIYAF----TANCMCMMVTGNH 286
Cdd:TIGR01733 124 YVIYTSGSTGRPKGVVVTHRSLV-NLLAWLA---------RRYGLDPDDRVLQ----FASLSFdasvEEIFGALLAGATL 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLITNP-RDIPGFIKELGKWRF-TALLGLNTLFVALMDHPGFrqlDFSALKVTNSGGTALVKATAERW-ESLTGCRIVE 363
Cdd:TIGR01733 190 VVPPEDEeRDDAALLAALIAEHPvTVLNLTPSLLALLAAALPP---ALASLRLVILGGEALTPALVDRWrARGPGARLIN 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 364 GYGLTETSPVASTNPY--GELARLGTVGI--PVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQ---- 435
Cdd:TIGR01733 267 LYGPTETTVWSTATLVdpDDAPRESPVPIgrPLANTRLYVLDDDLRPVPVGVVGELYIGGPGVARGYLNRPELTAErfvp 346
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 436 ----TLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:TIGR01733 347 dpfaGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
30-559 |
8.10e-49 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 176.38 E-value: 8.10e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqhTDLV-PGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPL 108
Cdd:cd17651 1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLR--ARGVgPGDLVALCARRSAELVVALLAILKAGAAYVPLDPA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 109 YTEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDLLPMAKGWLINTVVDKVKKMVPAYHlpqaisfk 188
Cdd:cd17651 79 YPAERLAFMLADAGPVLV---------------------LTHPALAGELAVELVAVTLLDQPGAAAGADAEP-------- 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 189 qalregaalshkPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQHGPDGQKLikegQEVMIA-PLPL 267
Cdd:cd17651 130 ------------DPALDADDLAYVIYTSGSTGRPKGVVMPHRSL-ANLVAWQARASSLGPGARTL----QFAGLGfDVSV 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIyaFTANCmcmmvTGNHNVLITNP--RDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTAL 345
Cdd:cd17651 193 QEI--FSTLC-----AGATLVLPPEEvrTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPLGVRLAALRYLLTGGEQL 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAER--WESLTGCRIVEGYGLTETSPV---ASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGP 420
Cdd:cd17651 266 VLTEDLRefCAGLPGLRLHNHYGPTETHVVtalSLPGDPAAWPAPPPIGRPIDNTRVYVLDAALRPVPPGVPGELYIGGA 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 421 QVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:cd17651 346 GLARGYLNRPELTAERFVPDPFVpgarmyRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVV 425
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 495 IGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd17651 426 LAREDRPGEKRLVAYVVGDPEaPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
50-562 |
9.05e-49 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 175.00 E-value: 9.05e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYTeremrhQFkdaGARALVYl 129
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSL-GVGKGDRVFVLSPRSPELYFSMLGIGKIGAVIC---PLFS------AF---GPEAIRD- 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 nmfgkRVQevlpdtgieyLIEAKMgdllpmakgwLINTvvdkvkkmvpayhlpqaisfkQALREgaalshkpvTLSLDDI 209
Cdd:cd05969 67 -----RLE----------NSEAKV----------LITT---------------------EELYE---------RTDPEDP 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 210 AVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqKLIKEGQEVMIAPLpLYHIYAFTANCMCMMVTGNHnvl 289
Cdd:cd05969 92 TLLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDLHPDD--IYWCTADPGWVTGT-VYGIWAPWLNGVTNVVYEGR--- 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 290 iTNPRDIPGFIKELG--KWRF--TALLGLNTLFVALMdhpgfRQLDFSALKVTNSGGTALvKATAERW-ESLTGCRIVEG 364
Cdd:cd05969 166 -FDAESWYGIIERVKvtVWYTapTAIRMLMKEGDELA-----RKYDLSSLRFIHSVGEPL-NPEAIRWgMEVFGVPIHDT 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 365 YGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKG--PQVMKGYWQQPEATAQTLdADGW 442
Cdd:cd05969 239 WWQTETGSIMIANYPCMPIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPgwPSMFRGIWNDEERYKNSF-IDGW 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 443 LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDEL 522
Cdd:cd05969 318 YLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDEL 397
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 820875961 523 K----AYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05969 398 KeeiiNFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
30-560 |
1.10e-48 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 176.87 E-value: 1.10e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 30 FERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPgDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLY 109
Cdd:PRK13382 49 FAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEP-RVVGIMCRNHRGFVEALLAANRIGADILLLNTSF 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 110 TEREMRHQFKDAGARALVYLNMFGKRVQEVLPDTGIEYLIEAkmgdllpmakgWLINTVVDKVKKMVPAyHLPQAIsfKQ 189
Cdd:PRK13382 128 AGPALAEVVTREGVDTVIYDEEFSATVDRALADCPQATRIVA-----------WTDEDHDLTVEVLIAA-HAGQRP--EP 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 190 ALREGaalshkpvtlsldDIAVLqyTGGTTGLAKGAmlthgnlvanmlqvlacfSQHGPDGQKLIK--------EGQEVM 261
Cdd:PRK13382 194 TGRKG-------------RVILL--TSGTTGTPKGA------------------RRSGPGGIGTLKaildrtpwRAEEPT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 262 IAPLPLYHIYAFT-----ANCMCMMVTGNHnvliTNPRDIPGFIKelgKWRFTALLGLNTLFVALMDHP--GFRQLDFSA 334
Cdd:PRK13382 241 VIVAPMFHAWGFSqlvlaASLACTIVTRRR----FDPEATLDLID---RHRATGLAVVPVMFDRIMDLPaeVRNRYSGRS 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 335 LKVTNSGGTAL----VKATAERWesltGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLG 410
Cdd:PRK13382 314 LRFAAASGSRMrpdvVIAFMDQF----GDVIYNNYNATEAGMIATATPADLRAAPDTAGRPAEGTEIRILDQDFREVPTG 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 411 ERGELCIKGPQVMKGYwqQPEATAQTLDadGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVA 490
Cdd:PRK13382 390 EVGTIFVRNDTQFDGY--TSGSTKDFHD--GFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVA 465
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 820875961 491 NCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK13382 466 EAAVIGVDDEQYGQRLAAFVVLKPGaSATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQA 536
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
27-558 |
1.77e-47 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 172.85 E-value: 1.77e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 27 VEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTN 106
Cdd:cd17646 1 HALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRAR-GVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 107 PLYTEREMRHQFKDAGARALVylnmfgkrVQEVLPDTGieylieakmgdllpmAKGWLINTVVDKVKKMVPAyHLPQAIS 186
Cdd:cd17646 80 PGYPADRLAYMLADAGPAVVL--------TTADLAARL---------------PAGGDVALLGDEALAAPPA-TPPLVPP 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 187 fkqalregaalshKPvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsQHGPDGQKLIKE--GQEVMIAP 264
Cdd:cd17646 136 -------------RP-----DNLAYVIYTSGSTGRPKGVMVTHAGIVNRLLWMQDEY-PLGPGDRVLQKTplSFDVSVWE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 265 L--PLyhiyaFTANCMCMMVTGNHnvliTNPRDIPGFIKElgkWRFTALLGLNTLFVALMDHPGFRQLDfsALKVTNSGG 342
Cdd:cd17646 197 LfwPL-----VAGARLVVARPGGH----RDPAYLAALIRE---HGVTTCHFVPSMLRVFLAEPAAGSCA--SLRRVFCSG 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 343 TALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGI--PVADTAFKVIDDEGNELPLGERGELCIKGP 420
Cdd:cd17646 263 EALPPELAARFLALPGAELHNLYGPTEAAIDVTHWPVRGPAETPSVPIgrPVPNTRLYVLDDALRPVPVGVPGELYLGGV 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 421 QVMKGYWQQPEATAQTLDADGW------LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:cd17646 343 QLARGYLGRPALTAERFVPDPFgpgsrmYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVV 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 495 IGVPDERSGEAVKLFVVAREGGLSVD--ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17646 423 VARAAPAGAARLVGYVVPAAGAAGPDtaALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
38-562 |
3.00e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 173.01 E-value: 3.00e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAFSNL---GKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREM 114
Cdd:PRK06164 21 RARPDAVALideDRPLSRAELRALVDRLAAWLAA-QGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEV 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 115 RHQFKDAGARALVYLNMFGKrvqevLPDTGIeylIEAKMGDLLPMAKGWLintVVDKVKKMVPAyHLPQAISFKQALREG 194
Cdd:PRK06164 100 AHILGRGRARWLVVWPGFKG-----IDFAAI---LAAVPPDALPPLRAIA---VVDDAADATPA-PAPGARVQLFALPDP 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 AALSHKPVTLSLDDIAVLQYT-GGTTGLAK------GAMLTHGNLVANMLQVlacfsqhGPDgqklikegqEVMIAPLPL 267
Cdd:PRK06164 168 APPAAAGERAADPDAGALLFTtSGTTSGPKlvlhrqATLLRHARAIARAYGY-------DPG---------AVLLAALPF 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIYAFtaNCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQlDFSALKVTN----SGGT 343
Cdd:PRK06164 232 CGVFGF--STLLGALAGGAPLVCEPVFDAARTARALRRHRVTHTFGNDEMLRRILDTAGERA-DFPSARLFGfasfAPAL 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 344 ALVKATAE-RWESLTGCrivegYGLTETSPVASTNPYG--ELARLGTVGIPVADTA-FKVID-DEGNELPLGERGELCIK 418
Cdd:PRK06164 309 GELAALARaRGVPLTGL-----YGSSEVQALVALQPATdpVSVRIEGGGRPASPEArVRARDpQDGALLPDGESGEIEIR 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 419 GPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVp 498
Cdd:PRK06164 384 APSLMRGYLDNPDATARALTDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA- 462
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 820875961 499 dERSGEAVKLFVVAREGGLSVDE--LKAYCKANFTGYKVPKHIVLRESLPMTPVG---KILRRELRDIA 562
Cdd:PRK06164 463 -TRDGKTVPVAFVIPTDGASPDEagLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMA 530
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
28-562 |
3.06e-47 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 172.87 E-value: 3.06e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKrfADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTnp 107
Cdd:PRK10946 29 DILTRHAA--SDAIAVICGERQFSYRELNQASDNLACSLRRQ-GIKPGDTALVQLGNVAEFYITFFALLKLGVAPVNA-- 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTERemRHQfkdagaralvyLNMFGKRVQEVLpdtgieyLIEAKMGDLLpmAKGWLINTVVDKVK--KMVPAYHLPQAI 185
Cdd:PRK10946 104 LFSHQ--RSE-----------LNAYASQIEPAL-------LIADRQHALF--SDDDFLNTLVAEHSslRVVLLLNDDGEH 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 186 SFKQALREGAALSHKPVTLSlDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQ-VLACfsQHGPDGQKLikegqevmiAP 264
Cdd:PRK10946 162 SLDDAINHPAEDFTATPSPA-DEVAFFQLSGGSTGTPKLIPRTHNDYYYSVRRsVEIC--GFTPQTRYL---------CA 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 265 LPLYHIYAFTA-NCMCMMVTGNHNVLITNPRDIPGF-IKELGKWRFTALL-GLNTLFVALMDHPGFRQlDFSALKVTNSG 341
Cdd:PRK10946 230 LPAAHNYPMSSpGALGVFLAGGTVVLAPDPSATLCFpLIEKHQVNVTALVpPAVSLWLQAIAEGGSRA-QLASLKLLQVG 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTALVKATAERWESLTGCRIVEGYGLTEtSPVASTnpygelaRL--------GTVGIPVA-DTAFKVIDDEGNELPLGER 412
Cdd:PRK10946 309 GARLSETLARRIPAELGCQLQQVFGMAE-GLVNYT-------RLddsderifTTQGRPMSpDDEVWVADADGNPLPQGEV 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 413 GELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANC 492
Cdd:PRK10946 381 GRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHA 460
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 820875961 493 AAIGVPDERSGEAVKLFVVAREgGLSVDELKAYCKA-NFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK10946 461 ALVSMEDELMGEKSCAFLVVKE-PLKAVQLRRFLREqGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWL 530
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
208-562 |
3.61e-47 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 167.51 E-value: 3.61e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGQKLIkegqevmiapLPLYHI--YAFTANCMcmmVTGN 285
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLS----------LPLYHVggLAILVRSL---LAGA 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HNVLITNPRDipgFIKELGKWRFTALLGLNTLFVALMDHPGFRQlDFSALKVTNSGGTALVKATAERWESLtGCRIVEGY 365
Cdd:cd17630 68 ELVLLERNQA---LAEDLAPPGVTHVSLVPTQLQRLLDSGQGPA-ALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTY 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 366 GLTETSPVASTNPYGELARlGTVGIPVadtafkviddEGNELPLGERGELCIKGPQVMKGYWQQPEAtaQTLDADGWLKT 445
Cdd:cd17630 143 GMTETASQVATKRPDGFGR-GGVGVLL----------PGRELRIVEDGEIWVGGASLAMGYLRGQLV--PEFNEDGWFTT 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 446 GDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAReGGLSVDELKAY 525
Cdd:cd17630 210 KDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGR-GPADPAELRAW 288
|
330 340 350
....*....|....*....|....*....|....*..
gi 820875961 526 CKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd17630 289 LKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
39-559 |
3.04e-46 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 169.80 E-value: 3.04e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAF--SNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRH 116
Cdd:PRK13390 12 DRPAVivAETGEQVSYRQLDDDSAALARVLYD-AGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADY 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 117 QFKDAGARALVylnmfgkrvqevlPDTGIEYLIEAKMGDL-LPMAKGWLINTVVDkvkkmvpayhlpqaisFKQALR-EG 194
Cdd:PRK13390 91 IVGDSGARVLV-------------ASAALDGLAAKVGADLpLRLSFGGEIDGFGS----------------FEAALAgAG 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 AALSHKPVTlslddiAVLQYTGGTTGLAKG--------AMLTHGNLVANMLQVLACFSQhgpdgqklikegQEVMIAPLP 266
Cdd:PRK13390 142 PRLTEQPCG------AVMLYSSGTTGFPKGiqpdlpgrDVDAPGDPIVAIARAFYDISE------------SDIYYSSAP 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 LYHIYAFTancMCMMVTGNHNVLITNPR-DIPGFIKELGKWRFTALLGLNTLFVALM--DHPGFRQLDFSALKVTNSGGT 343
Cdd:PRK13390 204 IYHAAPLR---WCSMVHALGGTVVLAKRfDAQATLGHVERYRITVTQMVPTMFVRLLklDADVRTRYDVSSLRAVIHAAA 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 344 AL---VKATAERWeslTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTaFKVIDDEGNELPLGERGELCIKGP 420
Cdd:PRK13390 281 PCpvdVKHAMIDW---LGPIVYEYYSSTEAHGMTFIDSPDWLAHPGSVGRSVLGD-LHICDDDGNELPAGRIGTVYFERD 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 421 QVMKGYWQQPEATA--QTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVP 498
Cdd:PRK13390 357 RLPFRYLNDPEKTAaaQHPAHPFWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVP 436
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 499 DERSGEAVKLFVVAREGGLSVDELKA----YCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK13390 437 DPEMGEQVKAVIQLVEGIRGSDELARelidYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
207-558 |
3.79e-46 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 169.32 E-value: 3.79e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQH-GPDgqklikegqEVMIAPLPLYHIYAFTANCMCMMvtgn 285
Cdd:cd17639 88 DDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRVPELlGPD---------DRYLAYLPLAHIFELAAENVCLY---- 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HNVLI--TNPRDIPGFIK-----ELGKWRFTALLG----LNTLFVALMDH----PGFRQLDFSA---------------- 334
Cdd:cd17639 155 RGGTIgyGSPRTLTDKSKrgckgDLTEFKPTLMVGvpaiWDTIRKGVLAKlnpmGGLKRTLFWTayqsklkalkegpgtp 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 335 -----------------LKVTNSGGTALVKATaERWESLTGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAF 397
Cdd:cd17639 235 lldelvfkkvraalggrLRYMLSGGAPLSADT-QEFLNIVLCPVIQGYGLTETCAGGTVQDPGDL-ETGRVGPPLPCCEI 312
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 398 KVID-DEGNELPLGE--RGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMI------- 467
Cdd:cd17639 313 KLVDwEEGGYSTDKPppRGEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKKDLVklqngey 392
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 468 -------IVSGFNVYPNEIedVIMGHPKVANCAAIGVPDErsgeaVKLFVVAREGGLSVDELKAYCK------------- 527
Cdd:cd17639 393 ialekleSIYRSNPLVNNI--CVYADPDKSYPVAIVVPNE-----KHLTKLAEKHGVINSEWEELCEdkklqkavlksla 465
|
410 420 430 440
....*....|....*....|....*....|....*....|..
gi 820875961 528 -----ANFTGYKVPKHIVLR------ESLPMTPVGKILRREL 558
Cdd:cd17639 466 etaraAGLEKFEIPQGVVLLdeewtpENGLVTAAQKLKRKEI 507
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
39-558 |
6.48e-46 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 167.85 E-value: 6.48e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd12116 2 DATAVRDDDRSLSYAELDERANRLAARLRARG-VGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrVQEVLPDTGIEYLieakmgdllpmakgwlintvvdkvkkMVPAYHLPQAisfkqalreGAALS 198
Cdd:cd12116 81 EDAEPALVL--------TDDALPDRLPAGL--------------------------PVLLLALAAA---------AAAPA 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVaNMLQVLACFSQHGPDGQklikegqevMIAPLPlyhiYAFTANCM 278
Cdd:cd12116 118 APRTPVSPDDLAYVIYTSGSTGRPKGVVVSHRNLV-NFLHSMRERLGLGPGDR---------LLAVTT----YAFDISLL 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CM---MVTGNHNVL-----ITNPRDIPGFIKELG---------KWRftaLLglntLFVALMDHPGFRQLdfsalkvtnSG 341
Cdd:cd12116 184 ELllpLLAGARVVIapretQRDPEALARLIEAHSitvmqatpaTWR---ML----LDAGWQGRAGLTAL---------CG 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTALVKATAERWESlTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQ 421
Cdd:cd12116 248 GEALPPDLAARLLS-RVGSLWNLYGPTETTIWSTAARVTAAAGPIPIGRPLANTQVYVLDAALRPVPPGVPGELYIGGDG 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 422 VMKGYWQQPEATAQTLDADG-------WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:cd12116 327 VAQGYLGRPALTAERFVPDPfagpgsrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAV 406
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 495 IGVPDERSGEAVKlFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd12116 407 VVREDGGDRRLVA-YVVLKAGaAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
38-559 |
1.48e-45 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 168.92 E-value: 1.48e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAFSNLGK----TLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVvntNPLYT--- 110
Cdd:PRK04319 58 KDKVALRYLDAsrkeKYTYKELKELSNKFANVLKEL-GVEKGDRVFIFMPRIPELYFALLGALKNGAIV---GPLFEafm 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 111 EREMRHQFKDAGARALVylnmfgkrvqevlpdTgIEYLIEAKMGDLLPMAKGWLintVVDKVKKMVPAYhlpqaISFKQA 190
Cdd:PRK04319 134 EEAVRDRLEDSEAKVLI---------------T-TPALLERKPADDLPSLKHVL---LVGEDVEEGPGT-----LDFNAL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 191 LREgAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANML---QVLacfsqhgpDgqklIKEGQevmiaplpl 267
Cdd:PRK04319 190 MEQ-ASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNAMLQHYQtgkYVL--------D----LHEDD--------- 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 yhIYAFTANCMcmMVTGN---------HNVliTN--------PRDIPGFIKELGK--WrFTAllglNTLFVALMDHPG-- 326
Cdd:PRK04319 248 --VYWCTADPG--WVTGTsygifapwlNGA--TNvidggrfsPERWYRILEDYKVtvW-YTA----PTAIRMLMGAGDdl 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 327 FRQLDFSALKVTNSGGTALvKATAERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGN 405
Cdd:PRK04319 317 VKKYDLSSLRHILSVGEPL-NPEVVRWgMKVFGLPIHDNWWMTETGGIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQGN 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKG--PQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVI 483
Cdd:PRK04319 396 ELPPNRMGNLAIKKgwPSMMRGIWNNPEKYESYF-AGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKL 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 484 MGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELK----AYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK04319 475 MEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKeeirGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLK 554
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
6-560 |
2.71e-45 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 171.96 E-value: 2.71e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKRPAgVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNV 85
Cdd:COG1020 465 WNATAAP-YPADATLH------ELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRAL-GVGPGDLVGVCLERS 536
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVntnPL---Y-TEReMRHQFKDAGARALVYlnmfGKRVQEVLPDTGIEYLieakmgdllpmak 161
Cdd:COG1020 537 LEMVVALLAVLKAGAAYV---PLdpaYpAER-LAYMLEDAGARLVLT----QSALAARLPELGVPVL------------- 595
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 162 gwlintVVDKvkkmvpayhlpqaisfkQALREGAALSHkPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLA 241
Cdd:COG1020 596 ------ALDA-----------------LALAAEPATNP-PVPVTPDDLAYVIYTSGSTGRPKGVMVEHRALVNLLAWMQR 651
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 242 CFSQHGPDgqklikegQEVMIAPlplyhiYAF---TANCMCMMVTGNHNVLITN--PRDIPGFIKELGKWRFTALLGLNT 316
Cdd:COG1020 652 RYGLGPGD--------RVLQFAS------LSFdasVWEIFGALLSGATLVLAPPeaRRDPAALAELLARHRVTVLNLTPS 717
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 317 LFVALMDHPGfrqLDFSALKVTNSGGTALVKATAERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLG---TVGIPV 392
Cdd:COG1020 718 LLRALLDAAP---EALPSLRLVLVGGEALPPELVRRWrARLPGARLVNLYGPTETTVDSTYYEVTPPDADGgsvPIGRPI 794
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 393 ADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQ-----TLDADG--WLKTGDIAVIDPDGFTRIVDRKKD 465
Cdd:COG1020 795 ANTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAErfvadPFGFPGarLYRTGDLARWLPDGNLEFLGRADD 874
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 466 MIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKAN-FTGYKVPKHIVLRES 544
Cdd:COG1020 875 QVKIRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALlLPPYMVPAAVVLLLP 954
|
570
....*....|....*.
gi 820875961 545 LPMTPVGKILRRELRD 560
Cdd:COG1020 955 LPLTGNGKLDRLALPA 970
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
8-561 |
7.24e-45 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 166.69 E-value: 7.24e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 8 DKRPAGVPSTIDihaytsvvEVFERSCKRFADR-PAFSNL---GKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMP 83
Cdd:cd05906 2 LHRPEGAPRTLL--------ELLLRAAERGPTKgITYIDAdgsEEFQSYQDLLEDARRLAAGLRQLG-LRPGDSVILQFD 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 84 NVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAgaralvylnmfgKRVQEVLPDTGIeyLIEAKM-GDLLPMAKG 162
Cdd:cd05906 73 DNEDFIPAFWACVLAGFVPAPLTVPPTYDEPNARLRKL------------RHIWQLLGSPVV--LTDAELvAEFAGLETL 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 163 WLintvvdkvkkmvpayHLPQAISFKQALREGAALSHKPvTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqvLAC 242
Cdd:cd05906 139 SG---------------LPGIRVLSIEELLDTAADHDLP-QSRPDDLALLMLTSGSTGFPKAVPLTHRNILAR----SAG 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 243 FSQHGPDGQklikegQEVMIAPLPLYHIYAFTancMCM---MVTGNHNV------LITNPRDipgFIKELGKWRFTALLG 313
Cdd:cd05906 199 KIQHNGLTP------QDVFLNWVPLDHVGGLV---ELHlraVYLGCQQVhvpteeILADPLR---WLDLIDRYRVTITWA 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 314 LNTLFVALMDH----PGFRqLDFSALKVTNSGGTALVKATAERWESLT---GCR---IVEGYGLTET------SPVASTN 377
Cdd:cd05906 267 PNFAFALLNDLleeiEDGT-WDLSSLRYLVNAGEAVVAKTIRRLLRLLepyGLPpdaIRPAFGMTETcsgviySRSFPTY 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 378 PYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDpDGFT 457
Cdd:cd05906 346 DHSQALEFVSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGWFRTGDLGFLD-NGNL 424
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 458 RIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVAN---CAAIGVPDERSGEAVKLFVV---AREGGLS--VDELKAYCKAN 529
Cdd:cd05906 425 TITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPsftAAFAVRDPGAETEELAIFFVpeyDLQDALSetLRAIRSVVSRE 504
|
570 580 590
....*....|....*....|....*....|....
gi 820875961 530 FTGykVPKHIV--LRESLPMTPVGKILRRELRDI 561
Cdd:cd05906 505 VGV--SPAYLIplPKEEIPKTSLGKIQRSKLKAA 536
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
31-562 |
6.47e-44 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 163.96 E-value: 6.47e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 31 ERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGlIVVNT-NPLY 109
Cdd:PRK08162 25 ERAAEVYPDRPAVIHGDRRRTWAETYARCRRLASALARR-GIGRGDTVAVLLPNIPAMVEAHFGVPMAG-AVLNTlNTRL 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 110 TEREMRHQFKDAGARALVYLNMFGKRVQEVLpdtgieylieAKMGDLLPMakgwlintVVDKVKKMVPAYHLPQAISFKQ 189
Cdd:PRK08162 103 DAASIAFMLRHGEAKVLIVDTEFAEVAREAL----------ALLPGPKPL--------VIDVDDPEYPGGRFIGALDYEA 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 190 ALREG-AALSHKPVTLSLDDIAvLQYTGGTTGLAKGAMLTH-GNLVANMLQVLAC-FSQHGpdgqklikegqeVMIAPLP 266
Cdd:PRK08162 165 FLASGdPDFAWTLPADEWDAIA-LNYTSGTTGNPKGVVYHHrGAYLNALSNILAWgMPKHP------------VYLWTLP 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 LYHiyaftANCMC----MMVTGNHNVLItnpRDI-PGFIKEL-GKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNS 340
Cdd:PRK08162 232 MFH-----CNGWCfpwtVAARAGTNVCL---RKVdPKLIFDLiREHGVTHYCGAPIVLSALINAPAEWRAGIDHPVHAMV 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 341 GGTALVKATAERWESLtGCRIVEGYGLTET-SPVA--------STNPYGELARL-GTVGIP-VADTAFKVID-DEGNELP 408
Cdd:PRK08162 304 AGAAPPAAVIAKMEEI-GFDLTHVYGLTETyGPATvcawqpewDALPLDERAQLkARQGVRyPLQEGVTVLDpDTMQPVP 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 409 L-GER-GELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGH 486
Cdd:PRK08162 383 AdGETiGEIMFRGNIVMKGYLKNPKATEEAF-AGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRH 461
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 487 PKVANCAAIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLREsLPMTPVGKILRRELRDIA 562
Cdd:PRK08162 462 PAVLVAAVVAKPDPKWGEVPCAFVELKDGaSATEEEIIAHCREHLAGFKVPKAVVFGE-LPKTSTGKIQKFVLREQA 537
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
50-559 |
9.00e-44 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 161.53 E-value: 9.00e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYTE---REMRHQFKDAGARAL 126
Cdd:cd05973 1 LTFGELRALSARFANALQEL-GVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQ---PLFTAfgpKAIEHRLRTSGARLV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VylnmfgkrvqevlpdtgieylieakmgdllpmakgwlinTVVDKVKKMVpayhlpqaisfkqalregaalshkpvtlsl 206
Cdd:cd05973 77 V---------------------------------------TDAANRHKLD------------------------------ 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGamlthgnlVANMLQVLACFSQHGPDGQKLIKEGQEVMIA-PLPLYHIY-AFTANcmcmMVTG 284
Cdd:cd05973 88 SDPFVMMFTSGTTGLPKG--------VPVPLRALAAFGAYLRDAVDLRPEDSFWNAAdPGWAYGLYyAITGP----LALG 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 285 NHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALM-------DHPGFRqldfsaLKVTNSGGTALvkaTAE--RWES 355
Cdd:cd05973 156 HPTILLEGGFSVESTWRVIERLGVTNLAGSPTAYRLLMaagaevpARPKGR------LRRVSSAGEPL---TPEviRWFD 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LT-GCRIVEGYGLTETS-PVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCI---KGPQV-MKGYWQQ 429
Cdd:cd05973 227 AAlGVPIHDHYGQTELGmVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIdiaNSPLMwFRGYQLP 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 430 PEATAqtldADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLF 509
Cdd:cd05973 307 DTPAI----DGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAF 382
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 510 VVAREG-----GLSvDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05973 383 VVLRGGhegtpALA-DELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLR 436
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
207-559 |
1.16e-43 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 163.43 E-value: 1.16e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcFSQHGPDgqklikegqEVMIAPLPLYHIYAFTAnCMCMMVTGNH 286
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIA-IVGYGED---------DVYLHTAPLCHIGGLSS-ALAMLMVGAC 240
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLItnprdiPGF-----IKELGKWRFTALLGLNTLFVAL-------MDHPGFRqldfSALKVTNSGGTALVKATAERWE 354
Cdd:PLN02860 241 HVLL------PKFdakaaLQAIKQHNVTSMITVPAMMADLisltrksMTWKVFP----SVRKILNGGGSLSSRLLPDAKK 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 SLTGCRIVEGYGLTET---------------SPVASTNPYGELARLGT-------VGIPVADTAFKVIDDEGNELplger 412
Cdd:PLN02860 311 LFPNAKLFSAYGMTEAcssltfmtlhdptleSPKQTLQTVNQTKSSSVhqpqgvcVGKPAPHVELKIGLDESSRV----- 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 413 GELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANC 492
Cdd:PLN02860 386 GRILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASV 465
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 493 AAIGVPDERSGEAVKLFVVAREG---------------GLSVDELKAYC-KANFTGYKVPKHIVL-RESLPMTPVGKILR 555
Cdd:PLN02860 466 VVVGVPDSRLTEMVVACVRLRDGwiwsdnekenakknlTLSSETLRHHCrEKNLSRFKIPKLFVQwRKPFPLTTTGKIRR 545
|
....
gi 820875961 556 RELR 559
Cdd:PLN02860 546 DEVR 549
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
26-559 |
1.63e-43 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 163.82 E-value: 1.63e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 26 VVEVFERSCKRFADRPAFSNLG-----KTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGL 100
Cdd:cd05968 63 VEQLLDKWLADTRTRPALRWEGedgtsRTLTYGELLYEVKRLANGLR-ALGVGKGDRVGIYLPMIPEIVPAFLAVARIGG 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 101 IVVNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLP----------DTGIEY-LIEAKMGDLLPMAKGWLIntvv 169
Cdd:cd05968 142 IVVPIFSGFGKEAAATRLQDAEAKALITADGFTRRGREVNLkeeadkacaqCPTVEKvVVVRHLGNDFTPAKGRDL---- 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 170 dkvkkmvpAYHLPQAISFKQALREGAalshkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsqHGPD 249
Cdd:cd05968 218 --------SYDEEKETAGDGAERTES-----------EDPLMIIYTSGTTGKPKGTVHVHAGFPLKAAQDMY----FQFD 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 250 gqklIKEGQEV--------MIAPLPLYHiyAFTANCMCMMVTGnhnvlitnprdIPGFIKELGKWRFTA-----LLGLN- 315
Cdd:cd05968 275 ----LKPGDLLtwftdlgwMMGPWLIFG--GLILGATMVLYDG-----------APDHPKADRLWRMVEdheitHLGLSp 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 316 TLFVALMDH--PGFRQLDFSALKVTNSGGTALvkaTAERWESL------TGCRIVEGYGLTETSP-VASTNPYGELARLG 386
Cdd:cd05968 338 TLIRALKPRgdAPVNAHDLSSLRVLGSTGEPW---NPEPWNWLfetvgkGRNPIINYSGGTEISGgILGNVLIKPIKPSS 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 387 TVGiPVADTAFKVIDDEGNELPlGERGELCIKGPQV--MKGYWQQPEATAQTL--DADGWLKTGDIAVIDPDGFTRIVDR 462
Cdd:cd05968 415 FNG-PVPGMKADVLDESGKPAR-PEVGELVLLAPWPgmTRGFWRDEDRYLETYwsRFDNVWVHGDFAYYDEEGYFYILGR 492
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 463 KKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG----GLSVDELKAYCKANFTGYKVPKH 538
Cdd:cd05968 493 SDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGvtptEALAEELMERVADELGKPLSPER 572
|
570 580
....*....|....*....|.
gi 820875961 539 IVLRESLPMTPVGKILRRELR 559
Cdd:cd05968 573 ILFVKDLPKTRNAKVMRRVIR 593
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
214-555 |
2.58e-43 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 157.18 E-value: 2.58e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 214 YTGGTTGLAKGAMLTHGNLVANMlqvlacfsqhgPDGQKLIK-EGQEVMIAPLPLYHIYAFTAnCMCMMVTGNhNVLITN 292
Cdd:cd17633 7 FTSGTTGLPKAYYRSERSWIESF-----------VCNEDLFNiSGEDAILAPGPLSHSLFLYG-AISALYLGG-TFIGQR 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 293 PRDIPGFIKELGKWRFTALLGLNTLFVALMDHpgfrQLDFSALKVTNSGGTALVKATAERWES-LTGCRIVEGYGLTETS 371
Cdd:cd17633 74 KFNPKSWIRKINQYNATVIYLVPTMLQALART----LEPESKIKSIFSSGQKLFESTKKKLKNiFPKANLIEFYGTSELS 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 372 PVaSTNPYGELARLGTVGIPVADTAFKVIDDEGnelplGERGELCIKGPQVMKGYwqqpeATAQTLDADGWLKTGDIAVI 451
Cdd:cd17633 150 FI-TYNFNQESRPPNSVGRPFPNVEIEIRNADG-----GEIGKIFVKSEMVFSGY-----VRGGFSNPDGWMSVGDIGYV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 452 DPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVARegGLSVDELKAYCKANFT 531
Cdd:cd17633 219 DEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYSGD--KLTYKQLKRFLKQKLS 296
|
330 340
....*....|....*....|....
gi 820875961 532 GYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd17633 297 RYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
22-558 |
1.15e-42 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 164.56 E-value: 1.15e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 22 AYTSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI 101
Cdd:PRK12467 510 APDCVHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAA-GVGPDVLVGIAVERSIEMVVGLLAVLKAGGA 588
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 102 VVNTNPLYTEREMRHQFKDAGARALvyLNMFGKRVQEVLPDtGIEYLIEAKMGDLLpmakgwlintvvdkvkkmvpayhl 181
Cdd:PRK12467 589 YVPLDPEYPQDRLAYMLDDSGVRLL--LTQSHLLAQLPVPA-GLRSLCLDEPADLL------------------------ 641
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 182 pqaisfkqalrEGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQHGPDGQKLikegqevM 261
Cdd:PRK12467 642 -----------CGYSGHNPEVALDPDNLAYVIYTSGSTGQPKGVAISHGAL-ANYVCVIAERLQLAADDSML-------M 702
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 262 IAPLP--LYHIYAFTANCmcmmvTGNhNVLITNP---RDIPGFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALK 336
Cdd:PRK12467 703 VSTFAfdLGVTELFGALA-----SGA-TLHLLPPdcaRDAEAFAALMADQGVTVLKIVPSHLQALLQAS--RVALPRPQR 774
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALVKATAERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLGT---VGIPVADTAFKVIDDEGNELPLGER 412
Cdd:PRK12467 775 ALVCGGEALQVDLLARVrALGPGARLINHYGPTETTVGVSTYELSDEERDFGnvpIGQPLANLGLYILDHYLNPVPVGVV 854
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 413 GELCIKGPQVMKGYWQQPEATAQTLDADGW-------LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMG 485
Cdd:PRK12467 855 GELYIGGAGLARGYHRRPALTAERFVPDPFgadggrlYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLA 934
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 486 HPKVANCAAIGVPDERSGEAVKLFVVAR-----EGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK12467 935 QPGVREAVVLAQPGDAGLQLVAYLVPAAvadgaEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
51-560 |
2.42e-42 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 159.53 E-value: 2.42e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP-LYTERemrhqfkdagaraLVYL 129
Cdd:PRK06018 41 TYAQIHDRALKVSQALDR-DGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPrLFPEQ-------------IAWI 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 -NMFGKRVqeVLPDTGIEYLIEaKMGDLLPMAKGWLINTvvDKvkKMVPAYHLPQAISFKQALrEGAALSHKPVTLSLDD 208
Cdd:PRK06018 107 iNHAEDRV--VITDLTFVPILE-KIADKLPSVERYVVLT--DA--AHMPQTTLKNAVAYEEWI-AEADGDFAWKTFDENT 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 209 IAVLQYTGGTTGLAKGAMLTHGnlvANMLQVLACfsqHGPDGqkLIKEGQEVMIAPLPLYHI----YAFTANCMcmmvtg 284
Cdd:PRK06018 179 AAGMCYTSGTTGDPKGVLYSHR---SNVLHALMA---NNGDA--LGTSAADTMLPVVPLFHAnswgIAFSAPSM------ 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 285 NHNVLITNPRDIPGFIKEL---GKWRFTAllGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLtGCRI 361
Cdd:PRK06018 245 GTKLVMPGAKLDGASVYELldtEKVTFTA--GVPTVWLMLLQYMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDM-GVEV 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 362 VEGYGLTETSPVAS----TNPYGEL---ARLG---TVGIPVADTAFKVIDDEGNELPLGER--GELCIKGPQVMKGYWQq 429
Cdd:PRK06018 322 RHAWGMTEMSPLGTlaalKPPFSKLpgdARLDvlqKQGYPPFGVEMKITDDAGKELPWDGKtfGRLKVRGPAVAAAYYR- 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 430 peATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLF 509
Cdd:PRK06018 401 --VDGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLI 478
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 820875961 510 VVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK06018 479 VQLKPGeTATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
6-558 |
4.88e-42 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 162.82 E-value: 4.88e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKrPAGVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNV 85
Cdd:PRK12316 4540 WNRT-DAGYPATRCVH------QLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIAR-GVGPEVLVGIAMERS 4611
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDLLPMAKGwLI 165
Cdd:PRK12316 4612 AEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALL---------------------LTQSHLLQRLPIPDG-LA 4669
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 NTVVDKVKKMvpayhlpqaisfkqalrEGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVaNMLQVLACFSQ 245
Cdd:PRK12316 4670 SLALDRDEDW-----------------EGFPAHDPAVRLHPDNLAYVIYTSGSTGRPKGVAVSHGSLV-NHLHATGERYE 4731
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 246 HGPDGQKLIKEGQEVMIAPLPLYHIYAfTANCMCMMVTGNHnvlitnprDIPGFIKELGKWRFTALLGLNTLFVALMDHP 325
Cdd:PRK12316 4732 LTPDDRVLQFMSFSFDGSHEGLYHPLI-NGASVVIRDDSLW--------DPERLYAEIHEHRVTVLVFPPVYLQQLAEHA 4802
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 326 GfRQLDFSALKVTNSGGTALVKATAER-WESLTGCRIVEGYGLTETS--PVASTNPYGELARLGTV--GIPVADTAFKVI 400
Cdd:PRK12316 4803 E-RDGEPPSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGPTETTvtVLLWKARDGDACGAAYMpiGTPLGNRSGYVL 4881
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 401 DDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQ-----TLDADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFN 473
Cdd:PRK12316 4882 DGQLNPLPVGVAGELYLGGEGVARGYLERPALTAErfvpdPFGAPGgrLYRTGDLARYRADGVIDYLGRVDHQVKIRGFR 4961
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 474 VYPNEIEDVIMGHPKVANCAAIGVPDErSGEAVKLFVVAREGGLS---------VDELKAYCKANFTGYKVPKHIVLRES 544
Cdd:PRK12316 4962 IELGEIEARLREHPAVREAVVIAQEGA-VGKQLVGYVVPQDPALAdadeaqaelRDELKAALRERLPEYMVPAHLVFLAR 5040
|
570
....*....|....
gi 820875961 545 LPMTPVGKILRREL 558
Cdd:PRK12316 5041 MPLTPNGKLDRKAL 5054
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
39-558 |
4.36e-41 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 153.95 E-value: 4.36e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd17652 2 DAPAVVFGDETLTYAELNARANRLARLLAAR-GVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlinTVVDKvkkmvPAYhlpqaisfkqalregaals 198
Cdd:cd17652 81 ADARPALLL---------------------------------------TTPDN-----LAY------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hkpvtlslddiaVLqYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQHGPDGQKLikegQevmiaplplYHIYAFTANC- 277
Cdd:cd17652 98 ------------VI-YTSGSTGRPKGVVVTHRGL-ANLAAAQIAAFDVGPGSRVL----Q---------FASPSFDASVw 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 278 -MCM-MVTGNHNVLITNPRDIPG--FIKELGKWRFTALL----GLNTLFVAlmDHPGFRQLdfsalkvtNSGGTALVKAT 349
Cdd:cd17652 151 eLLMaLLAGATLVLAPAEELLPGepLADLLREHRITHVTlppaALAALPPD--DLPDLRTL--------VVAGEACPAEL 220
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 350 AERWEslTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQ 429
Cdd:cd17652 221 VDRWA--PGRRMINAYGPTETTVCATMAGPLPGGGVPPIGRPVPGTRVYVLDARLRPVPPGVPGELYIAGAGLARGYLNR 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 430 PEATAQTLDADGW-------LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANcAAIGVPDERS 502
Cdd:cd17652 299 PGLTAERFVADPFgapgsrmYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAE-AVVVVRDDRP 377
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 503 GEA--VKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17652 378 GDKrlVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
48-554 |
9.63e-41 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 155.81 E-value: 9.63e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALV 127
Cdd:cd17634 83 RTISYRELHREVCRFAGTLLD-LGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLI 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 ylnmfgkrvqevlpdTGIEYLIEAKMGDLLPMAKGWL------INTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKP 201
Cdd:cd17634 162 ---------------TADGGVRAGRSVPLKKNVDDALnpnvtsVEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQP 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 202 VTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLA-CFSQHGPDGQKLIKEGQEVMIAPLPLYHIYAF------- 273
Cdd:cd17634 227 EAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKyVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACgattlly 306
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 274 -------TANCMCMMVTgNH--NVLITNPRDIPGFIKELGKWrftallglntlfvalmdhpgFRQLDFSALKVTNSGGTA 344
Cdd:cd17634 307 egvpnwpTPARMWQVVD-KHgvNILYTAPTAIRALMAAGDDA--------------------IEGTDRSSLRILGSVGEP 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 345 LVKATAE-RWESLTG--CRIVEGYGLTETSPVASTN-PYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKG- 419
Cdd:cd17634 366 INPEAYEwYWKKIGKekCPVVDTWWQTETGGFMITPlPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDp 445
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 420 -PQVMKGYWQQPEATAQTLDA--DGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIG 496
Cdd:cd17634 446 wPGQTRTLFGDHERFEQTYFStfKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVG 525
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 497 VPDERSGEAVKLFVVAREG-----GLSvDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKIL 554
Cdd:cd17634 526 IPHAIKGQAPYAYVVLNHGvepspELY-AELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
28-558 |
1.87e-40 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 153.25 E-value: 1.87e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:cd17655 1 ELFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREK-GVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEremrhqfkdagaralvylnmfgKRVQEVLPDTGIEYLIEAKmgdllpmakgwlintvvdkvkkmvpayHLPQAISF 187
Cdd:cd17655 80 DYPE----------------------ERIQYILEDSGADILLTQS---------------------------HLQPPIAF 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 --------KQALREGAALSHKPVTLSlDDIAVLQYTGGTTGLAKGAMLTHGNLVaNMLQVLacfsqhgpdgQKLIKEGQE 259
Cdd:cd17655 111 iglidlldEDTIYHEESENLEPVSKS-DDLAYVIYTSGSTGKPKGVMIEHRGVV-NLVEWA----------NKVIYQGEH 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 260 VMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNPRDIPG--FIKELGKWRFTALLGLNTLFVAL--MDHpgfrqLDFSAL 335
Cdd:cd17655 179 LRVALFASISFDASVTEIFASLLSGNTLYIVRKETVLDGqaLTQYIRQNRITIIDLTPAHLKLLdaADD-----SEGLSL 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 336 KVTNSGGTALVKATAERWESL--TGCRIVEGYGLTETSPVASTNPYGELARLGT---VGIPVADTAFKVIDDEGNELPLG 410
Cdd:cd17655 254 KHLIVGGEALSTELAKKIIELfgTNPTITNAYGPTETTVDASIYQYEPETDQQVsvpIGKPLGNTRIYILDQYGRPQPVG 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 411 ERGELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIM 484
Cdd:cd17655 334 VAGELYIGGEGVARGYLNRPELTAEKFVDDPFVpgermyRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLL 413
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 485 GHPKVANCAAIGVPDERSGEAVKLFVVAREgGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17655 414 QHPDIKEAVVIARKDEQGQNYLCAYIVSEK-ELPVAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
26-562 |
4.68e-40 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 151.93 E-value: 4.68e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 26 VVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVnt 105
Cdd:cd05918 1 VHDLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSL-GVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFV-- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 nPLyteremrhqfkDAGARAlvylnmfgKRVQEVLPDTGIeylieakmgdllpmakgwlinTVVdkvkkmvpayhlpqai 185
Cdd:cd05918 78 -PL-----------DPSHPL--------QRLQEILQDTGA---------------------KVV---------------- 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 186 sfkqalregaaLSHKPvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQ------------VLaCFSQHGPDgqkl 253
Cdd:cd05918 101 -----------LTSSP-----SDAAYVIFTSGSTGKPKGVVIEHRALSTSALAhgralgltsesrVL-QFASYTFD---- 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 254 ikegqeVMIAPlplyHIYAFTAN-CMCmmVTGNHNvlITNprDIPGFIKELGkwrftallgLNTLF----VALMDHPGfr 328
Cdd:cd05918 160 ------VSILE----IFTTLAAGgCLC--IPSEED--RLN--DLAGFINRLR---------VTWAFltpsVARLLDPE-- 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 329 qlDFSALKVTNSGGTALVKATAERWESltGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFkVID--DEGNE 406
Cdd:cd05918 213 --DVPSLRTLVLGGEALTQSDVDTWAD--RVRLINAYGPAECTIAATVSPVVPSTDPRNIGRPLGATCW-VVDpdNHDRL 287
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 407 LPLGERGELCIKGPQVMKGYWQQPEATAQT-LDADGWL------------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFN 473
Cdd:cd05918 288 VPIGAVGELLIEGPILARGYLNDPEKTAAAfIEDPAWLkqegsgrgrrlyRTGDLVRYNPDGSLEYVGRKDTQVKIRGQR 367
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 474 VYPNEIEDVIMGH-PKVANCAAIGVPDERSGEAVKL--FVVAREGGLS------------------VDELKAYCKANFTG 532
Cdd:cd05918 368 VELGEIEHHLRQSlPGAKEVVVEVVKPKDGSSSPQLvaFVVLDGSSSGsgdgdslflepsdefralVAELRSKLRQRLPS 447
|
570 580 590
....*....|....*....|....*....|
gi 820875961 533 YKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05918 448 YMVPSVFLPLSHLPLTASGKIDRRALRELA 477
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
212-560 |
1.77e-39 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 151.40 E-value: 1.77e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 212 LQYTGGTTGLAKGAMLTHGNlvanmlQVLACFSQHGPDGQKLikEGQEVMIAPLPLYHIYAFTANCMCMMvTGNHNVLit 291
Cdd:PRK07008 181 LCYTSGTTGNPKGALYSHRS------TVLHAYGAALPDAMGL--SARDAVLPVVPMFHVNAWGLPYSAPL-TGAKLVL-- 249
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 292 nprdiPGfiKEL-GKW--------RFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIV 362
Cdd:PRK07008 250 -----PG--PDLdGKSlyelieaeRVTFSAGVPTVWLGLLNHMREAGLRFSTLRRTVIGGSACPPAMIRTFEDEYGVEVI 322
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 363 EGYGLTETSPVAST----------NPYGELARLGTVGIPVADTAFKVIDDEGNELPLGER--GELCIKGPQVMKGYWQQp 430
Cdd:PRK07008 323 HAWGMTEMSPLGTLcklkwkhsqlPLDEQRKLLEKQGRVIYGVDMKIVGDDGRELPWDGKafGDLQVRGPWVIDRYFRG- 401
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 431 eaTAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVP----DERSgeav 506
Cdd:PRK07008 402 --DASPLV-DGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAhpkwDERP---- 474
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 507 kLFVVAREGGLSV--DELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07008 475 -LLVVVKRPGAEVtrEELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLRE 529
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
208-555 |
1.94e-39 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 147.02 E-value: 1.94e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMlqvlacfsqhgpdgQKLIKEG-----QEVMIAPLPLYHIYAFTANCMCMM- 281
Cdd:cd17635 2 DPLAVIFTSGTTGEPKAVLLANKTFFAVP--------------DILQKEGlnwvvGDVTYLPLPATHIGGLWWILTCLIh 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 ----VTGNHNVLITNprdipgFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLT 357
Cdd:cd17635 68 gglcVTGGENTTYKS------LFKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRLIGYGGSRAIAADVRFIEATG 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 358 GCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTL 437
Cdd:cd17635 142 LTNTAQVYGLSETGTALCLPTDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVL 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 438 dADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREggl 517
Cdd:cd17635 222 -IDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASA--- 297
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 820875961 518 SVDE-----LKAYCKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd17635 298 ELDEnairaLKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
28-560 |
9.56e-39 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 147.46 E-value: 9.56e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnP 107
Cdd:cd17653 1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLG-VVPGDVVPLLSDRSLEMLVAILAILKAGAAYV---P 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LyteremrhqfkDAGARAlvylnmfgKRVQEVLPDTGIEYLIEAKmgdllpmakgwlintvvdkvkkmvpayhlpqaisf 187
Cdd:cd17653 77 L-----------DAKLPS--------ARIQAILRTSGATLLLTTD----------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 kqalregaalshkpvtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLA-CFSQHGPdgqkliKEGQevMIAPlp 266
Cdd:cd17653 103 -----------------SPDDLAYIIFTSGSTGIPKGVMVPHRGVLNYVSQPPArLDVGPGS------RVAQ--VLSI-- 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 lyhiyAFTANCMCMMVT---GNHNVLITNPRDIPGFIKELGkwrftallglntlfvALMDHPGF----RQLDFSALKVTN 339
Cdd:cd17653 156 -----AFDACIGEIFSTlcnGGTLVLADPSDPFAHVARTVD---------------ALMSTPSIlstlSPQDFPNLKTIF 215
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 340 SGGTALVKATAERWesLTGCRIVEGYGLTETSpVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKG 419
Cdd:cd17653 216 LGGEAVPPSLLDRW--SPGRRLYNAYGPTECT-ISSTMTELLPGQPVTIGKPIPNSTCYILDADLQPVPEGVVGEICISG 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 420 PQVMKGYWQQPEATAQ----TLDADGWL--KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd17653 293 VQVARGYLGNPALTASkfvpDPFWPGSRmyRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQA 372
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 494 AIGVpderSGEAVKLFVVARegGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:cd17653 373 AAIV----VNGRLVAFVTPE--TVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
28-558 |
2.52e-38 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 146.31 E-value: 2.52e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:cd12115 3 DLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLR-AAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisf 187
Cdd:cd12115 82 AYPPERLRFILEDAQARLVL------------------------------------------------------------ 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 kqalregaalshkpvtLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhgpdgqklikegqEVMIAPLpl 267
Cdd:cd12115 102 ----------------TDPDDLAYVIYTSGSTGRPKGVAIEHRNAAAFLQWAAAAFSA-------------EELAGVL-- 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 yhiyAFTANC--------MCMMVTGNHNVLITNPRDIPGFIKELGkwrfTALLglNT---LFVALMDHPGFRQldfsALK 336
Cdd:cd12115 151 ----ASTSICfdlsvfelFGPLATGGKVVLADNVLALPDLPAAAE----VTLI--NTvpsAAAELLRHDALPA----SVR 216
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALVKATAER-WESLTGCRIVEGYGLTETSpVASTNPYGELARLGTVGI--PVADTAFKVIDDEGNELPLGERG 413
Cdd:cd12115 217 VVNLAGEPLPRDLVQRlYARLQVERVVNLYGPSEDT-TYSTVAPVPPGASGEVSIgrPLANTQAYVLDRALQPVPLGVPG 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 414 ELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHP 487
Cdd:cd12115 296 ELYIGGAGVARGYLGRPGLTAERFLPDPFGpgarlyRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIP 375
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 488 KVANCAAIGVPDERSGEAVKLFVVA-REGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd12115 376 GVREAVVVAIGDAAGERRLVAYIVAePGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
39-558 |
6.22e-38 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 145.53 E-value: 6.22e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd17643 2 EAVAVVDEDRRLTYGELDARANRLARTLRAE-GVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrvqeVLPDTgieylieakmgdllpmakgwlintvvdkvkkmvPAYhlpqaisfkqalregaals 198
Cdd:cd17643 81 ADSGPSLLL-----------TDPDD---------------------------------LAY------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hkpvtlslddiaVLqYTGGTTGLAKGAMLTHGNlvanmlqVLACFSQHGPDgqkLIKEGQEVMIaplpLYHIYAFTANCM 278
Cdd:cd17643 98 ------------VI-YTSGSTGRPKGVVVSHAN-------VLALFAATQRW---FGFNEDDVWT----LFHSYAFDFSVW 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CM---MVTGNHNVLITN-----PRDIPGFIKELGkwrFTALLGLNTLFVALM---DHPGFRQLdfsALKVTNSGGTALVK 347
Cdd:cd17643 151 EIwgaLLHGGRLVVVPYevarsPEDFARLLRDEG---VTVLNQTPSAFYQLVeaaDRDGRDPL---ALRYVIFGGEALEA 224
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 348 ATAERW---ESLTGCRIVEGYGLTETSPVAS----TNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGP 420
Cdd:cd17643 225 AMLRPWagrFGLDRPQLVNMYGITETTVHVTfrplDAADLPAAAASPIGRPLPGLRVYVLDADGRPVPPGVVGELYVSGA 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 421 QVMKGYWQQPEATAQ-------TLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd17643 305 GVARGYLGRPELTAErfvanpfGGPGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAA 384
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 494 AIGVPDERSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17643 385 VIVREDEPGDTRLVAYVVADDGaAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
48-562 |
1.13e-37 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 145.31 E-value: 1.13e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHTDLvpGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALV 127
Cdd:PRK07638 25 RVLTYKDWFESVCKVANWLNEKESK--NKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMIV 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 YLNMFGKRVQEVlpDTGIeylieakmgdllpmakgWLIntvvDKVKKMVPAYhlpqaisfKQALREGAALSHKPVTLSld 207
Cdd:PRK07638 103 TERYKLNDLPDE--EGRV-----------------IEI----DEWKRMIEKY--------LPTYAPIENVQNAPFYMG-- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 diavlqYTGGTTGLAKGAMLTHGNLVANmlqvlacFSQHGPDGQklIKEGQEVMIaPLPLYH---IYA-----FTANCMC 279
Cdd:PRK07638 150 ------FTSGSTGKPKAFLRAQQSWLHS-------FDCNVHDFH--MKREDSVLI-AGTLVHslfLYGaistlYVGQTVH 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 280 MMvtgnhnvlitnPRDIPGFIKE-LGKWRFTALLGLNTLFVALMDHPGFRQldfSALKVTNSG---GTALVKATAERWES 355
Cdd:PRK07638 214 LM-----------RKFIPNQVLDkLETENISVMYTVPTMLESLYKENRVIE---NKMKIISSGakwEAEAKEKIKNIFPY 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LtgcRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYwQQPEATAQ 435
Cdd:PRK07638 280 A---KLYEFYGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGY-IIGGVLAR 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 436 TLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVvarEG 515
Cdd:PRK07638 356 ELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII---KG 432
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 820875961 516 GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK07638 433 SATKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
50-496 |
2.20e-37 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 144.92 E-value: 2.20e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYteremrhqfKDAGARALVYl 129
Cdd:cd05932 7 FTWGEVADKARRLAAALR-ALGLEPGSKIALISKNCAEWFITDLAIWMAGHISV---PLY---------PTLNPDTIRY- 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 nmfgkrvqeVLPDTGIEYLIEAKMGDLLPMAKGwlintVVDKVKKMVPAYH--LPQAISFKQALREGAALSHKPVTLSlD 207
Cdd:cd05932 73 ---------VLEHSESKALFVGKLDDWKAMAPG-----VPEGLISISLPPPsaANCQYQWDDLIAQHPPLEERPTRFP-E 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLacfsqhgpdgQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGnhn 287
Cdd:cd05932 138 QLATLIYTSGTTGQPKGVMLTFGSFAWAAQAGI----------EHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGG--- 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPRDIPGFIKELGKWRFTALLGLNTLFV-------------------------ALMDHPGFRQLDFSALKVTNSGG 342
Cdd:cd05932 205 VLVAFAESLDTFVEDVQRARPTLFFSVPRLWTkfqqgvqdkipqqklnlllkipvvnSLVKRKVLKGLGLDQCRLAGCGS 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 343 TALVKATAERWESLtGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVadtafkviddEGNELPLGERGELCIKGPQV 422
Cdd:cd05932 285 APVPPALLEWYRSL-GLNILEAYGMTENFAYSHLNYPGR-DKIGTVGNAG----------PGVEVRISEDGEILVRSPAL 352
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 820875961 423 MKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPNEIEDVIMGHPKVANCAAIG 496
Cdd:cd05932 353 MMGYYKDPEATAEAFTADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
207-553 |
2.62e-37 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 147.80 E-value: 2.62e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIYAFTANCMCMMVTGNH 286
Cdd:PRK06814 793 DDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPED----------KVFNALPVFHSFGLTGGLVLPLLSGVK 862
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLITNP---RDIPGFIKELGKwrfTALLGLNTLFV--ALMDHPgfrqLDFSALKVTNSGGTALVKATAERWESLTGCRI 361
Cdd:PRK06814 863 VFLYPSPlhyRIIPELIYDTNA---TILFGTDTFLNgyARYAHP----YDFRSLRYVFAGAEKVKEETRQTWMEKFGIRI 935
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 362 VEGYGLTETSPVASTN-PYGElaRLGTVG--IPVADTafKVIDDEGNElplgERGELCIKGPQVMKGYWQ-------QPE 431
Cdd:PRK06814 936 LEGYGVTETAPVIALNtPMHN--KAGTVGrlLPGIEY--RLEPVPGID----EGGRLFVRGPNVMLGYLRaenpgvlEPP 1007
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 432 ataqtldADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVV 511
Cdd:PRK06814 1008 -------ADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDARKGERIILLTT 1080
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 820875961 512 ARegGLSVDELKAYCKAN-FTGYKVPKHIVLRESLPMTPVGKI 553
Cdd:PRK06814 1081 AS--DATRAAFLAHAKAAgASELMVPAEIITIDEIPLLGTGKI 1121
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
48-562 |
3.15e-37 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 145.92 E-value: 3.15e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI--VVNTNplYTEREMRHQFKDAGARA 125
Cdd:cd05967 81 RTYTYAELLDEVSRLAGVLRKL-GVVKGDRVIIYMPMIPEAAIAMLACARIGAIhsVVFGG--FAAKELASRIDDAKPKL 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 126 LVYLNmFGKRvqevlPDTGIEYLieaKMGDLLPMAKGWLINTVVDKVKKMVPAYHLPQAISFK-QALREGAAlSHKPVTL 204
Cdd:cd05967 158 IVTAS-CGIE-----PGKVVPYK---PLLDKALELSGHKPHHVLVLNRPQVPADLTKPGRDLDwSELLAKAE-PVDCVPV 227
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 SLDDIAVLQYTGGTTGLAKGAML-THGNLVANMLQVLACFSQHGPD------------GQKLIKEGqevmiaplPLYHiy 271
Cdd:cd05967 228 AATDPLYILYTSGTTGKPKGVVRdNGGHAVALNWSMRNIYGIKPGDvwwaasdvgwvvGHSYIVYG--------PLLH-- 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 272 aftancmcmmvtGNHNVLI----TNPRDIPGFIKELGKWRFTALLGLNTLFVAL-MDHPG---FRQLDFSALKVTNSGGT 343
Cdd:cd05967 298 ------------GATTVLYegkpVGTPDPGAFWRVIEKYQVNALFTAPTAIRAIrKEDPDgkyIKKYDLSSLRTLFLAGE 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 344 ALVKATAERWESLTGCRIVEGYGLTET-SPVAsTNPYGeLA----RLGTVGIPVADTAFKVIDDEGNELPLGERGELCIK 418
Cdd:cd05967 366 RLDPPTLEWAENTLGVPVIDHWWQTETgWPIT-ANPVG-LEplpiKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIK 443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 419 GP---QVMKGYWQQPEATAQTL--DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd05967 444 LPlppGCLLTLWKNDERFKKLYlsKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECA 523
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 494 AIGVPDERSGEAVKLFVVAREGGLSVD-----ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05967 524 VVGVRDELKGQVPLGLVVLKEGVKITAeelekELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIA 597
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
28-527 |
2.42e-36 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 143.70 E-value: 2.42e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAfsnLG------------KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGA 95
Cdd:PLN02736 48 DNFVYAVETFRDYKY---LGtrirvdgtvgeyKWMTYGEAGTARTAIGSGLVQH-GIPKGACVGLYFINRPEWLIVDHAC 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 96 LRAGLIVVntnPLYteremrhqfkDA-GARALVYL-NMFGKRVQEVLPDTgieylIEAKMGDLLPMAKGWLInTVVDKVK 173
Cdd:PLN02736 124 SAYSYVSV---PLY----------DTlGPDAVKFIvNHAEVAAIFCVPQT-----LNTLLSCLSEIPSVRLI-VVVGGAD 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 174 KMVPAyhLPQA-----ISFKQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMlqvlACFSQhgp 248
Cdd:PLN02736 185 EPLPS--LPSGtgveiVTYSKLLAQGRSSPQPFRPPKPEDVATICYTSGTTGTPKGVVLTHGNLIANV----AGSSL--- 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 249 dgqkLIKEG-QEVMIAPLPLYHIYAFTANCMCM-------------------MVTGNHNVLITNPR-------DIPGFIK 301
Cdd:PLN02736 256 ----STKFYpSDVHISYLPLAHIYERVNQIVMLhygvavgfyqgdnlklmddLAALRPTIFCSVPRlynriydGITNAVK 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 302 ELGKWR---FTAllGLNTLFVALMD----HPGFRQLDFSALK--------VTNSGGTALvkaTAERWESLT---GCRIVE 363
Cdd:PLN02736 332 ESGGLKerlFNA--AYNAKKQALENgknpSPMWDRLVFNKIKaklggrvrFMSSGASPL---SPDVMEFLRicfGGRVLE 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 364 GYGLTETSPVASTNPYGELArLGTVGIPVADTAFKVID-------DEGNELPlgeRGELCIKGPQVMKGYWQQPEATAQT 436
Cdd:PLN02736 407 GYGMTETSCVISGMDEGDNL-SGHVGSPNPACEVKLVDvpemnytSEDQPYP---RGEICVRGPIIFKGYYKDEVQTREV 482
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 437 LDADGWLKTGDIAVIDPDGFTRIVDRKKDMI-IVSGFNVYPNEIEDVIMGHPKVANC-----------AAIGVPDERSge 504
Cdd:PLN02736 483 IDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENVYAKCKFVAQCfvygdslnsslVAVVVVDPEV-- 560
|
570 580
....*....|....*....|...
gi 820875961 505 avkLFVVAREGGLSVDELKAYCK 527
Cdd:PLN02736 561 ---LKAWAASEGIKYEDLKQLCN 580
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
39-559 |
2.92e-36 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 140.58 E-value: 2.92e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd17649 2 DAVALVFGDQSLSYAELDARANRLAHRLRAL-GVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALvylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaaLS 198
Cdd:cd17649 81 EDSGAGLL----------------------------------------------------------------------LT 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTLslddiAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGQ-----------------KLIkEGQEVM 261
Cdd:cd17649 91 HHPRQL-----AYVIYTSGSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRElqfasfnfdgaheqllpPLI-CGACVV 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 262 IAPLPLYHiyafTANCMCMMVTgNHNVLITnprDIPgfikelgkwrfTALLglnTLFVALMDHPGFRqlDFSALKVTNSG 341
Cdd:cd17649 165 LRPDELWA----SADELAEMVR-ELGVTVL---DLP-----------PAYL---QQLAEEADRTGDG--RPPSLRLYIFG 220
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTALVKATAERWESlTGCRIVEGYGLTET--SPVASTNPYGElARLGT---VGIPVADTAFKVIDDEGNELPLGERGELC 416
Cdd:cd17649 221 GEALSPELLRRWLK-APVRLFNAYGPTEAtvTPLVWKCEAGA-ARAGAsmpIGRPLGGRSAYILDADLNPVPVGVTGELY 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQTL--DADG-----WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKV 489
Cdd:cd17649 299 IGGEGLARGYLGRPELTAERFvpDPFGapgsrLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGV 378
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 490 ANCAAIGVPDERSGEAVKLFV--VAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd17649 379 REAAVVALDGAGGKQLVAYVVlrAAAAQPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
28-558 |
6.49e-36 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 139.61 E-value: 6.49e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:cd17645 2 QLFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRG-KGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALVylnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisf 187
Cdd:cd17645 81 DYPGERIAYMLADSSAKILL------------------------------------------------------------ 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 KQAlregaalshkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhGPDGQKLIkegqevmiaplpl 267
Cdd:cd17645 101 TNP----------------DDLAYVIYTSGSTGLPKGVMIEHHNLVNLCEWHRPYFGV-TPADKSLV------------- 150
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIYAFTANCMCMM--VTGNHNVLITnPRDIPGFIKELGKWRFTALLGLNTLFVALMDHpgFRQLDFSALKVTNSGGTAL 345
Cdd:cd17645 151 YASFSFDASAWEIFphLTAGAALHVV-PSERRLDLDALNDYFNQEGITISFLPTGAAEQ--FMQLDNQSLRVLLTGGDKL 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAErwesltGCRIVEGYGLTETSPVAST----NPYGELarlgTVGIPVADTAFKVIDdEGNEL-PLGERGELCIKGP 420
Cdd:cd17645 228 KKIERK------GYKLVNNYGPTENTVVATSfeidKPYANI----PIGKPIDNTRVYILD-EALQLqPIGVAGELCIAGE 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 421 QVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAA 494
Cdd:cd17645 297 GLARGYLNRPELTAEKFIVHPFVpgermyRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAV 376
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 495 IGVPDERSGEAVKLFVVAREgGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17645 377 LAKEDADGRKYLVAYVTAPE-EIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
6-558 |
8.23e-36 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 143.94 E-value: 8.23e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNdKRPAGVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNV 85
Cdd:PRK12316 500 WN-ATAAEYPLQRGVH------RLFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIER-GVGPDVLVGVAMERS 571
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEremrhqfkdagaralvylnmfgKRVQEVLPDTGIEYLI-EAKMGDLLPMAKGwL 164
Cdd:PRK12316 572 IEMVVALLAILKAGGAYVPLDPEYPA----------------------ERLAYMLEDSGVQLLLsQSHLGRKLPLAAG-V 628
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 165 INTVVDKVKKMVPAYHlpqaisfkqalregaalSHKPVT-LSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMlqvlaCF 243
Cdd:PRK12316 629 QVLDLDRPAAWLEGYS-----------------EENPGTeLNPENLAYVIYTSGSTGKPKGAGNRHRALSNRL-----CW 686
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 244 SQhgpdgQKLIKEGQEVMIAPLPlyhiYAFTANCMCM---MVTGNHNVLIT--NPRDIPGFIKELGKWRFTALLGLNTLF 318
Cdd:PRK12316 687 MQ-----QAYGLGVGDTVLQKTP----FSFDVSVWEFfwpLMSGARLVVAApgDHRDPAKLVELINREGVDTLHFVPSML 757
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 319 VALMDHPGFRQLDfsALKVTNSGGTALVKATAER-WESLTGCRIVEGYGLTETS-PVASTNPYGELARLGTVGIPVADTA 396
Cdd:PRK12316 758 QAFLQDEDVASCT--SLRRIVCSGEALPADAQEQvFAKLPQAGLYNLYGPTEAAiDVTHWTCVEEGGDSVPIGRPIANLA 835
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 397 FKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVS 470
Cdd:PRK12316 836 CYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFVagermyRTGDLARYRADGVIEYAGRIDHQVKLR 915
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 471 GFNVYPNEIEDVIMGHPKVANCAAIGVpderSGEAVKLFVVAR-EGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTP 549
Cdd:PRK12316 916 GLRIELGEIEARLLEHPWVREAAVLAV----DGKQLVGYVVLEsEGGDWREALKAHLAASLPEYMVPAQWLALERLPLTP 991
|
....*....
gi 820875961 550 VGKILRREL 558
Cdd:PRK12316 992 NGKLDRKAL 1000
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
199-562 |
1.15e-35 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 140.92 E-value: 1.15e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTLSlddiavlqYTGGTTGLAKGAMLTHGNLVANMLQVLAcfsqhgpdgqklikeGQEVMIAP-----LPLYHIYAF 273
Cdd:PLN03102 186 HDPISLN--------YTSGTTADPKGVVISHRGAYLSTLSAII---------------GWEMGTCPvylwtLPMFHCNGW 242
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 274 TANcMCMMVTGNHNVLITNPrDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERW 353
Cdd:PLN03102 243 TFT-WGTAARGGTSVCMRHV-TAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPVHVLTGGSPPPAALVKKV 320
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 354 ESLtGCRIVEGYGLTE-TSPVA-----------STNPYGEL-ARLGTVGIPVADTAFKVIDDEGNELPLGER-GELCIKG 419
Cdd:PLN03102 321 QRL-GFQVMHAYGLTEaTGPVLfcewqdewnrlPENQQMELkARQGVSILGLADVDVKNKETQESVPRDGKTmGEIVIKG 399
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 420 PQVMKGYWQQPEATAQTLDaDGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPD 499
Cdd:PLN03102 400 SSIMKGYLKNPKATSEAFK-HGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPH 478
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 500 ERSGEAVKLFVVAREGGLSVDE-----------LKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PLN03102 479 PTWGETPCAFVVLEKGETTKEDrvdklvtrerdLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIA 552
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
6-558 |
5.45e-35 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 141.45 E-value: 5.45e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNdKRPAGVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNV 85
Cdd:PRK12467 1563 WN-ATHTGYPLARLVH------QLIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIAL-GVGPEVLVGIAVERS 1634
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDLLPMAKGwli 165
Cdd:PRK12467 1635 LEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELL---------------------LTQSHLQARLPLPDG--- 1690
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 ntvvdkvkkmVPAYHLPQAisfkQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVAnmlqvLACFSQ 245
Cdd:PRK12467 1691 ----------LRSLVLDQE----DDWLEGYSDSNPAVNLAPQNLAYVIYTSGSTGRPKGAGNRHGALVN-----RLCATQ 1751
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 246 HG----PDGQKLIKegqevmiaplplyHIYAFTANC---MCMMVTGNhNVLITNP---RDIPGFIKELGKWRFTALLGLN 315
Cdd:PRK12467 1752 EAyqlsAADVVLQF-------------TSFAFDVSVwelFWPLINGA-RLVIAPPgahRDPEQLIQLIERQQVTTLHFVP 1817
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 316 TLFVALMDHPGfRQLDFSALKVTNSGGTAL-VKATAERWESLTGCRIVEGYGLTETS------PVASTNPYGELArlGTV 388
Cdd:PRK12467 1818 SMLQQLLQMDE-QVEHPLSLRRVVCGGEALeVEALRPWLERLPDTGLFNLYGPTETAvdvthwTCRRKDLEGRDS--VPI 1894
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 389 GIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGW-------LKTGDIAVIDPDGFTRIVD 461
Cdd:PRK12467 1895 GQPIANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFgtvgsrlYRTGDLARYRADGVIEYLG 1974
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 462 RKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVpDERSGEAVKLFVVAREGGL---------SVDELKAYCKANFTG 532
Cdd:PRK12467 1975 RIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQ-DGANGKQLVAYVVPTDPGLvdddeaqvaLRAILKNHLKASLPE 2053
|
570 580
....*....|....*....|....*.
gi 820875961 533 YKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK12467 2054 YMVPAHLVFLARMPLTPNGKLDRKAL 2079
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
176-559 |
6.61e-35 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 134.79 E-value: 6.61e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 176 VPAYHLPQAISFKQALREGAALShkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqVLACFSQHGPDGQKLIK 255
Cdd:PRK07824 12 VPAQDERRAALLRDALRVGEPID--------DDVALVVATSGTTGTPKGAMLTAAALTAS---ADATHDRLGGPGQWLLA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 256 egqevmiapLPLYHIYAFTAnCMCMMVTGNHNVLIT-----NPRDIPGFIKEL-GKWRFTALLGLNtLFVALMDHPGFRQ 329
Cdd:PRK07824 81 ---------LPAHHIAGLQV-LVRSVIAGSEPVELDvsagfDPTALPRAVAELgGGRRYTSLVPMQ-LAKALDDPAATAA 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 330 L-DFSALKVtnsGGTALVKATAERWESLtGCRIVEGYGLTETSPvastnpygelarlGTV--GIPVADTAFKVIDdegne 406
Cdd:PRK07824 150 LaELDAVLV---GGGPAPAPVLDAAAAA-GINVVRTYGMSETSG-------------GCVydGVPLDGVRVRVED----- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 407 lplgerGELCIKGPQVMKGYWQQPEATAqtLDADGWLKTGDIAVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGH 486
Cdd:PRK07824 208 ------GRIALGGPTLAKGYRNPVDPDP--FAEPGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATH 278
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 487 PKVANCAAIGVPDERSGEAVKLFVVAREGGL-SVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK07824 279 PAVADCAVFGLPDDRLGQRVVAAVVGDGGPApTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALV 352
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
205-552 |
1.08e-34 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 134.43 E-value: 1.08e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 SLDDIAVLqYTGGTTGLAKGAMLTHGNL------VANMlqVLACFSQHGPDGQKLIKEGQEVMIAPLPLYH----IYAFT 274
Cdd:cd05924 2 SADDLYIL-YTGGTTGMPKGVMWRQEDIfrmlmgGADF--GTGEFTPSEDAHKAAAAAAGTVMFPAPPLMHgtgsWTAFG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 275 AncmcmmVTGNHNVLITNPRDIPgfiKELgkWRFTALLGLNTLFVA--LMDHP------GFRQLDFSALKVTNSGGTALV 346
Cdd:cd05924 79 G------LLGGQTVVLPDDRFDP---EEV--WRTIEKHKVTSMTIVgdAMARPlidalrDAGPYDLSSLFAISSGGALLS 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 347 KATAERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAfkVIDDEGNELPLGE--RGELCIKGpQVM 423
Cdd:cd05924 148 PEVKQGLlELVPNITLVDAFGSSETGFTGSGHSAGSGPETGPFTRANPDTV--VLDDDGRVVPPGSggVGWIARRG-HIP 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 424 KGYWQQPEATAQTL-DADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDE 500
Cdd:cd05924 225 LGYYGDEAKTAETFpEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDE 304
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 820875961 501 RSGEAVKLFVVAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGK 552
Cdd:cd05924 305 RWGQEVVAVVQLREGaGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
191-557 |
1.69e-34 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 137.05 E-value: 1.69e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 191 LREGAALshKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACfSQHGPDgqklikegQEVMIAPLPLYHI 270
Cdd:PRK07768 138 LLAADPI--DPVETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVA-AEFDVE--------TDVMVSWLPLFHD 206
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 271 YAFTANCMCMMVTGNHNVLITnP----RDIPGFIKELGKWRFTALLGLN---TLFVALM---DHPGfrQLDFSALKVTNS 340
Cdd:PRK07768 207 MGMVGFLTVPMYFGAELVKVT-PmdflRDPLLWAELISKYRGTMTAAPNfayALLARRLrrqAKPG--AFDLSSLRFALN 283
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 341 GGTALVKATAERWESlTGCR-------IVEGYGLTETSPVASTNPYGE-----------LA--------------RLGTV 388
Cdd:PRK07768 284 GAEPIDPADVEDLLD-AGARfglrpeaILPAYGMAEATLAVSFSPCGAglvvdevdadlLAalrravpatkgntrRLATL 362
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 389 GIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYwQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMII 468
Cdd:PRK07768 363 GPPLPGLEVRVVDEDGQVLPPRGVGVIELRGESVTPGY-LTMDGFIPAQDADGWLDTGDLGYLTEEGEVVVCGRVKDVII 441
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 469 VSGFNVYPNEIEDVIMGHPKV--ANCAAIGVPDERSGEAvklFVVARE--GGLSVDELKAYCKAnfTGYKV-------PK 537
Cdd:PRK07768 442 MAGRNIYPTDIERAAARVEGVrpGNAVAVRLDAGHSREG---FAVAVEsnAFEDPAEVRRIRHQ--VAHEVvaevgvrPR 516
|
410 420
....*....|....*....|..
gi 820875961 538 HIVLRE--SLPMTPVGKiLRRE 557
Cdd:PRK07768 517 NVVVLGpgSIPKTPSGK-LRRA 537
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
48-557 |
2.46e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 137.00 E-value: 2.46e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYTEREMRHQfkdagaralv 127
Cdd:PRK05850 34 ETLTWSQLYRRTLNVAEELRRHGS--TGDRAVILAPQGLEYIVAFLGALQAGLIAV---PLSVPQGGAHD---------- 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 ylnmfgKRVQEVLPDTGieylieakmgdllPMAkgwLINT--VVDKVKKMVPAYHLPQAISFKQ--ALREGAALSHKPVT 203
Cdd:PRK05850 99 ------ERVSAVLRDTS-------------PSV---VLTTsaVVDDVTEYVAPQPGQSAPPVIEvdLLDLDSPRGSDARP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 204 LSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGQKLikegQEVMIAPLPLYHIYAFTANCMCMMVT 283
Cdd:PRK05850 157 RDLPSTAYLQYTSGSTRTPAGVMVSHRNVIANFEQLMSDYFGDTGGVPPP----DTTVVSWLPFYHDMGLVLGVCAPILG 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 284 GNHNVLiTNPRdipGFIKELGKWrfTALLGLNTL-FVALmdhPGFrQLDFSALKVTNS-------GGTALVKATAERWES 355
Cdd:PRK05850 233 GCPAVL-TSPV---AFLQRPARW--MQLLASNPHaFSAA---PNF-AFELAVRKTSDDdmagldlGGVLGIISGSERVHP 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LTGCRIVE--------------GYGLTE-TSPVASTNP----------YGELA-------------RLGTVGIPvADTAF 397
Cdd:PRK05850 303 ATLKRFADrfapfnlretairpSYGLAEaTVYVATREPgqppesvrfdYEKLSaghakrcetgggtPLVSYGSP-RSPTV 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 398 KVIDDEGN-ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDA----------DG-WLKTGDIAVIDPDGFTrIVDRKKD 465
Cdd:PRK05850 382 RIVDPDTCiECPAGTVGEIWVHGDNVAAGYWQKPEETERTFGAtlvdpspgtpEGpWLRTGDLGFISEGELF-IVGRIKD 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 466 MIIVSGFNVYPNEIEDVIMGHPKvANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYC--KANFTGYKVPKH----- 538
Cdd:PRK05850 461 LLIVDGRNHYPDDIEATIQEITG-GRVAAISVPDDGTEKLVAIIELKKRGDSDEEAMDRLRtvKREVTSAISKSHglsva 539
|
570 580
....*....|....*....|..
gi 820875961 539 ---IVLRESLPMTPVGKILRRE 557
Cdd:PRK05850 540 dlvLVAPGSIPITTSGKIRRAA 561
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
39-558 |
3.18e-34 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 135.48 E-value: 3.18e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLyteremrhqf 118
Cdd:cd12114 2 DATAVICGDGTLTYGELAERARRVAGALKAAG-VRPGDLVAVTLPKGPEQVVAVLGILAAGAAYV---PV---------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 kDAGARALvylnmfgkRVQEVLPDTGIeylieakmgDLLPMAKGWLINTVVdkvkkmvpayhLPQAISFKQALREGAALS 198
Cdd:cd12114 68 -DIDQPAA--------RREAILADAGA---------RLVLTDGPDAQLDVA-----------VFDVLILDLDALAAPAPP 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHG---NLVANMLQ---------VLACFSQH---------GPdgqkLIKEG 257
Cdd:cd12114 119 -PPVDVAPDDLAYVIFTSGSTGTPKGVMISHRaalNTILDINRrfavgpddrVLALSSLSfdlsvydifGA----LSAGA 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 258 QEVMIAPLplyhiYAFTANCMCMMVTgNHNVLITNprDIPgfikelgkwrftALLGLntlfvaLMDHPGFRQLDFSALK- 336
Cdd:cd12114 194 TLVLPDEA-----RRRDPAHWAELIE-RHGVTLWN--SVP------------ALLEM------LLDVLEAAQALLPSLRl 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSG---GTALVKATAERWeslTGCRIVEGYGLTETS------PVASTN------PYGelarlgtvgIPVADTAFKVID 401
Cdd:cd12114 248 VLLSGdwiPLDLPARLRALA---PDARLISLGGATEASiwsiyhPIDEVPpdwrsiPYG---------RPLANQRYRVLD 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 402 DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTL--DADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPN 477
Cdd:cd12114 316 PRGRDCPDWVPGELWIGGRGVALGYLGDPELTAARFvtHPDGerLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELG 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 478 EIEDVIMGHPKVANCAAIGVpDERSGEAVKLFVVAREGG--LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:cd12114 396 EIEAALQAHPGVARAVVVVL-GDPGGKRLAAFVVPDNDGtpIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDR 474
|
...
gi 820875961 556 REL 558
Cdd:cd12114 475 AAL 477
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
24-483 |
7.57e-34 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 137.99 E-value: 7.57e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFSNLGKT------LTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQYPIAVFGALR 97
Cdd:PRK05691 9 LTLVQALQRRAAQTPDRLALRFLADDpgegvvLSYRDLDLRARTIAAALQARAS--FGDRAVLLFPSGPDYVAAFFGCLY 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 98 AGLIVVNTNPLYTEREmRHQ------FKDAGARALVYLNMFGKRVQEvlpdtgieylIEAKMGDLLPmakGWLintVVDk 171
Cdd:PRK05691 87 AGVIAVPAYPPESARR-HHQerllsiIADAEPRLLLTVADLRDSLLQ----------MEELAAANAP---ELL---CVD- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 172 vkkmvpayhlpqaisfkqALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFS-QHGPDg 250
Cdd:PRK05691 149 ------------------TLDPALAEAWQEPALQPDDIAFLQYTSGSTALPKGVQVSHGNLVANEQLIRHGFGiDLNPD- 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 251 qklikegqEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITnprdiPGF--------IKELGKWRFTALLGLN---TLFV 319
Cdd:PRK05691 210 --------DVIVSWLPLYHDMGLIGGLLQPIFSGVPCVLMS-----PAYflerplrwLEAISEYGGTISGGPDfayRLCS 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 320 ALMDHPGFRQLDFSALKVTNSGGTALVKATAERW-ESLTGCRIVE-----GYGLTETSPVASTNPYGE-----------L 382
Cdd:PRK05691 277 ERVSESALERLDLSRWRVAYSGSEPIRQDSLERFaEKFAACGFDPdsffaSYGLAEATLFVSGGRRGQgipaleldaeaL 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 383 AR----------LGTVGIPVADTAFKVID-DEGNELPLGERGELCIKGPQVMKGYWQQPEATAQT---LDADGWLKTGDI 448
Cdd:PRK05691 357 ARnraepgtgsvLMSCGRSQPGHAVLIVDpQSLEVLGDNRVGEIWASGPSIAHGYWRNPEASAKTfveHDGRTWLRTGDL 436
|
490 500 510
....*....|....*....|....*....|....*
gi 820875961 449 AVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVI 483
Cdd:PRK05691 437 GFLR-DGELFVTGRLKDMLIVRGHNLYPQDIEKTV 470
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
207-558 |
1.35e-33 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 132.98 E-value: 1.35e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNlVANMlqVLACFSQHGPDGQklikegqEVMIAPLPLYHIYAFTANCMCMMVTGNh 286
Cdd:cd17650 93 EDLAYVIYTSGTTGKPKGVMVEHRN-VAHA--AHAWRREYELDSF-------PVRLLQMASFSFDVFAGDFARSLLNGG- 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 nVLITNPRDI---PGFIKE-LGKWRFTALLGLNTLFVALMDHPGFRQLDFSALK---VTNSGGTALVKATAERwESLTGC 359
Cdd:cd17650 162 -TLVICPDEVkldPAALYDlILKSRITLMESTPALIRPVMAYVYRNGLDLSAMRlliVGSDGCKAQDFKTLAA-RFGQGM 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 360 RIVEGYGLTETSpVASTNPYGELARLGT-----VGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATA 434
Cdd:cd17650 240 RIINSYGVTEAT-IDSTYYEEGRDPLGDsanvpIGRPLPNTAMYVLDERLQPQPVGVAGELYIGGAGVARGYLNRPELTA 318
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 435 QTLDADGW------LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANcAAIGVPDERSGEA-VK 507
Cdd:cd17650 319 ERFVENPFapgermYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDE-AVVAVREDKGGEArLC 397
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 820875961 508 LFVVAREgGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17650 398 AYVVAAA-TLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
51-562 |
1.44e-33 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 134.59 E-value: 1.44e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 51 TYGELDRLSAAFAawlqqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLiVVNTNPLYTEREMRHQFKDAGARALVYLN 130
Cdd:PLN02479 51 TYQRCRRLASALA-----KRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGA-VVNCVNIRLNAPTIAFLLEHSKSEVVMVD 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 131 mfgkrvQEVLP--DTGIEYLIEAKMGDLLPMakgwLINTVVDKVKKMVP-AYHLPQ-AISFKQALREG-AALSHKPVTLS 205
Cdd:PLN02479 125 ------QEFFTlaEEALKILAEKKKSSFKPP----LLIVIGDPTCDPKSlQYALGKgAIEYEKFLETGdPEFAWKPPADE 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 206 LDDIAvLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhgPDGQklikegqeVMIAPLPLYHI----YAFTANCMCmm 281
Cdd:PLN02479 195 WQSIA-LGYTSGTTASPKGVVLHHRGAYLMALSNALIWGM--NEGA--------VYLWTLPMFHCngwcFTWTLAALC-- 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 vtgNHNVLI--TNPRDIPGFIKELGKWRFTAL-LGLNTLFVALMDHpgfRQLDFSALKVTNSGGTA----LVKATAERwe 354
Cdd:PLN02479 262 ---GTNICLrqVTAKAIYSAIANYGVTHFCAApVVLNTIVNAPKSE---TILPLPRVVHVMTAGAApppsVLFAMSEK-- 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 sltGCRIVEGYGLTET---------SPVASTNPYGELARL---------GTVGIPVADT-AFKVIDDEGNELplgerGEL 415
Cdd:PLN02479 334 ---GFRVTHTYGLSETygpstvcawKPEWDSLPPEEQARLnarqgvryiGLEGLDVVDTkTMKPVPADGKTM-----GEI 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 416 CIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAI 495
Cdd:PLN02479 406 VMRGNMVMKGYLKNPKANEEAF-ANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVV 484
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 496 GVPDERSGEAVKLFVVAREGGLSVDE------LKAYCKANFTGYKVPKHIVLrESLPMTPVGKILRRELRDIA 562
Cdd:PLN02479 485 ARPDERWGESPCAFVTLKPGVDKSDEaalaedIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKHVLRAKA 556
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
48-454 |
3.55e-33 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 133.33 E-value: 3.55e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRH-QFKDAGAR-- 124
Cdd:cd05921 24 RRVTYAEALRQVRAIAQGLLDL-GLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVSPAYSLMSQDLaKLKHLFELlk 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 125 -ALVYLN---MFGKRVQEVLPDtgieylieakmgdllpmakgwlintvvdkvkkmvpayHLPQAISFKQALREG----AA 196
Cdd:cd05921 103 pGLVFAQdaaPFARALAAIFPL-------------------------------------GTPLVVSRNAVAGRGaisfAE 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 197 LSHKPVTLSL---------DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegQEVMIAPLPL 267
Cdd:cd05921 146 LAATPPTAAVdaafaavgpDTVAKFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEE--------PPVLVDWLPW 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIYAFTANCMCMMVTGNhNVLITNPRDIPGFIKELGK--------WRFTALLGLNTLFVALMDHPGFRQLDFSALKVTN 339
Cdd:cd05921 218 NHTFGGNHNFNLVLYNGG-TLYIDDGKPMPGGFEETLRnlreisptVYFNVPAGWEMLVAALEKDEALRRRFFKRLKLMF 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 340 SGGTALVKATAERWESL----TGCRI--VEGYGLTETSPvASTNPYGELARLGTVGIPVADTAFKVIddegnelPLGERG 413
Cdd:cd05921 297 YAGAGLSQDVWDRLQALavatVGERIpmMAGLGATETAP-TATFTHWPTERSGLIGLPAPGTELKLV-------PSGGKY 368
|
410 420 430 440
....*....|....*....|....*....|....*....|..
gi 820875961 414 ELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGD-IAVIDPD 454
Cdd:cd05921 369 EVRVKGPNVTPGYWRQPELTAQAFDEEGFYCLGDaAKLADPD 410
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
18-480 |
5.89e-33 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 133.32 E-value: 5.89e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 18 IDIHAYTSVVEVFERSCKRFADRPAFSNLGKT---------LTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQY 88
Cdd:PRK07769 15 IRFPPNTNLVRHVERWAKVRGDKLAYRFLDFSterdgvardLTWSQFGARNRAVGARLQQVTK--PGDRVAILAPQNLDY 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 89 PIAVFGALRAGLIVVntnPLYTEREMRHqfkdagaralvylnmfGKRVQEVLPD---TGIEYLIEAKMGdllpmakgwli 165
Cdd:PRK07769 93 LIAFFGALYAGRIAV---PLFDPAEPGH----------------VGRLHAVLDDctpSAILTTTDSAEG----------- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 ntvVDKVKKMVPAYHLPQAISFkQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsq 245
Cdd:PRK07769 143 ---VRKFFRARPAKERPRVIAV-DAVPDEVGATWVPPEANEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDAL-- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 246 hgpdgqklikEGQEVM--IAPLPLYHIYAFTANCMCMMvtGNHNVLITNPRdipGFIKELGKW-RFTALLGLNTL--FVA 320
Cdd:PRK07769 217 ----------EGQEGDrgVSWLPFFHDMGLITVLLPAL--LGHYITFMSPA---AFVRRPGRWiRELARKPGGTGgtFSA 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 321 L----MDHPGFR--------QLDFSALKVTNSGGTALVKATAERWES------LTGCRIVEGYGLTETSPVASTNPYGEL 382
Cdd:PRK07769 282 ApnfaFEHAAARglpkdgepPLDLSNVKGLLNGSEPVSPASMRKFNEafapygLPPTAIKPSYGMAEATLFVSTTPMDEE 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 383 ARLGTVG---------IPVADTA----------------FKVIDD--EGNELPLGERGELCIKGPQVMKGYWQQPEATAQ 435
Cdd:PRK07769 362 PTVIYVDrdelnagrfVEVPADApnavaqvsagkvgvseWAVIVDpeTASELPDGQIGEIWLHGNNIGTGYWGKPEETAA 441
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 436 TL-----------------DADGWLKTGDIAVIdPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PRK07769 442 TFqnilksrlseshaegapDDALWVRTGDYGVY-FDGELYITGRVKDLVIIDGRNHYPQDLE 502
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
207-559 |
6.87e-33 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 131.84 E-value: 6.87e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLacfsqhgpdgQKLIKEGQEVMIAPLPLYHIYAFTA-NCMCMMVTGN 285
Cdd:cd05908 106 DELAFIQFSSGSTGDPKGVMLTHENLVHNMFAIL----------NSTEWKTKDRILSWMPLTHDMGLIAfHLAPLIAGMN 175
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HNVLITNP--RDIPGFIKELGKWRFTALLGLN---TLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAErwESLTGCR 360
Cdd:cd05908 176 QYLMPTRLfiRRPILWLKKASEHKATIVSSPNfgyKYFLKTLKPEKANDWDLSSIRMILNGAEPIDYELCH--EFLDHMS 253
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 361 --------IVEGYGLTETSPVASTNPYG---------------------------ELARLGTVGIPVADTAFKVIDDEGN 405
Cdd:cd05908 254 kyglkrnaILPVYGLAEASVGASLPKAQspfktitlgrrhvthgepepevdkkdsECLTFVEVGKPIDETDIRICDEDNK 333
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMG 485
Cdd:cd05908 334 ILPDGYIGHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIR-NGRLVITGREKDIIFVNGQNVYPHDIERIAEE 412
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 486 HPKV--ANCAAIGVPDERS-GEAVKLFVVAREgglSVDELKAYCKA------NFTGYKVPKHIVLREsLPMTPVGKILRR 556
Cdd:cd05908 413 LEGVelGRVVACGVNNSNTrNEEIFCFIEHRK---SEDDFYPLGKKikkhlnKRGGWQINEVLPIRR-IPKTTSGKVKRY 488
|
...
gi 820875961 557 ELR 559
Cdd:cd05908 489 ELA 491
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
49-559 |
1.72e-32 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 131.44 E-value: 1.72e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 49 TLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVY 128
Cdd:PRK05620 38 QTTFAAIGARAAALAHALHDELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVA 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 129 LNMFGKRVQEVLPDT-GIEYLIEAKMGDllpmakgwlintvVDKvkkmvPAYHLPQAI---SFKQALREGAALSHKPVtL 204
Cdd:PRK05620 118 DPRLAEQLGEILKECpCVRAVVFIGPSD-------------ADS-----AAAHMPEGIkvySYEALLDGRSTVYDWPE-L 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 SLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFS---QHGpdgqklikegqEVMIAPLPLYHIYAFTANCMCMM 281
Cdd:PRK05620 179 DETTAAAICYSTGTTGAPKGVVYSHRSLYLQSLSLRTTDSlavTHG-----------ESFLCCVPIYHVLSWGVPLAAFM 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 vTGNHNVLITNPRDIPgfikELGKWRFTAL----LGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESLT 357
Cdd:PRK05620 248 -SGTPLVFPGPDLSAP----TLAKIIATAMprvaHGVPTLWIQLMVHYLKNPPERMSLQEIYVGGSAVPPILIKAWEERY 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 358 GCRIVEGYGLTETSPVastnpygelarlGTVGIPVADTA-------------------FKVIDDeGNELPLGER--GELC 416
Cdd:PRK05620 323 GVDVVHVWGMTETSPV------------GTVARPPSGVSgearwayrvsqgrfpasleYRIVND-GQVMESTDRneGEIQ 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQP----------------EATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PRK05620 390 VRGNWVTASYYHSPteegggaastfrgedvEDANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLE 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 481 DVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG----GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRR 556
Cdd:PRK05620 470 NYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGieptRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKK 549
|
...
gi 820875961 557 ELR 559
Cdd:PRK05620 550 DLR 552
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
36-558 |
2.92e-32 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 130.12 E-value: 2.92e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 36 RFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMR 115
Cdd:PRK13383 47 RWPGRTAIIDDDGALSYRELQRATESLARRLTR-DGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALA 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 116 HQFKDAGARALVYLNMFGKRVqevlpdtgieylieAKMGDLLpmakgwlinTVVDkvkkmvpayhlPQAISFKQAL-REG 194
Cdd:PRK13383 126 AALRAHHISTVVADNEFAERI--------------AGADDAV---------AVID-----------PATAGAEESGgRPA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 AALSHKPVTLslddiavlqyTGGTTGLAKGamLTHGNLVANMLQVLACFSQhgpdgQKLIKEGQEVMIApLPLYHIYAFT 274
Cdd:PRK13383 172 VAAPGRIVLL----------TSGTTGKPKG--VPRAPQLRSAVGVWVTILD-----RTRLRTGSRISVA-MPMFHGLGLG 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 275 ANCMCMMVTGNhnVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPG-FRQLD-FSALKVTNSGGTALVKATAER 352
Cdd:PRK13383 234 MLMLTIALGGT--VLTHRHFDAEAALAQASLHRADAFTAVPVVLARILELPPrVRARNpLPQLRVVMSSGDRLDPTLGQR 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 353 WESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNelPLGER--GELCIKGPQVMKGYwqqp 430
Cdd:PRK13383 312 FMDTYGDILYNGYGSTEVGIGALATPADLRDAPETVGKPVAGCPVRILDRNNR--PVGPRvtGRIFVGGELAGTRY---- 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 431 EATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFV 510
Cdd:PRK13383 386 TDGGGKAVVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFV 465
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 820875961 511 VAREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK13383 466 VLHPGsGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
31-562 |
4.41e-32 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 130.76 E-value: 4.41e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 31 ERSCKRFADRPAF------SNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI--V 102
Cdd:cd05966 60 DRHLKERGDKVAIiwegdePDQSRTITYRELLREVCRFANVLKSL-GVKKGDRVAIYMPMIPELVIAMLACARIGAVhsV 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNplYTEREMRHQFKDAGARALVYLNMF---GKRVQ------EVLPDT-GIEYLIEAK-MGDLLPMAKGwlINTVVDK 171
Cdd:cd05966 139 VFAG--FSAESLADRINDAQCKLVITADGGyrgGKVIPlkeivdEALEKCpSVEKVLVVKrTGGEVPMTEG--RDLWWHD 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 172 VKKMVPAYHLPQAISfkqalregaalshkpvtlSLDDIAVLqYTGGTTGLAKGAMLTHGnlvANMLQVLACFsqhgpdgq 251
Cdd:cd05966 215 LMAKQSPECEPEWMD------------------SEDPLFIL-YTSGSTGKPKGVVHTTG---GYLLYAATTF-------- 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 252 KL---IKEGQevmiaplplyhIYAFTANCMcmMVTGnHNVLITNP-------------RDIPGF-----IKElgKWRFTA 310
Cdd:cd05966 265 KYvfdYHPDD-----------IYWCTADIG--WITG-HSYIVYGPlangattvmfegtPTYPDPgrywdIVE--KHKVTI 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 311 LLGLNTLFVALM----DHPgfRQLDFSALKVTNSGGTALvkaTAERWE---SLTG---CRIVEGYGLTET-----SPVAS 375
Cdd:cd05966 329 FYTAPTAIRALMkfgdEWV--KKHDLSSLRVLGSVGEPI---NPEAWMwyyEVIGkerCPIVDTWWQTETggimiTPLPG 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 376 TNPygelARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKG--PQVMKGYWQQPEATAQTLDAD--GWLKTGDIAVI 451
Cdd:cd05966 404 ATP----LKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRpwPGMARTIYGDHERYEDTYFSKfpGYYFTGDGARR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 452 DPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFT 531
Cdd:cd05966 480 DEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDELRKELRKHVR 559
|
570 580 590
....*....|....*....|....*....|....*
gi 820875961 532 ----GYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:cd05966 560 keigPIATPDKIQFVPGLPKTRSGKIMRRILRKIA 594
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
39-559 |
7.51e-32 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 130.54 E-value: 7.51e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSnLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:PRK06060 21 DRPAFY-AADVVTHGQIHDGAARLGEVLRNR-GLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAA 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGArALVYLNMfgkrvqevlpdtgieylieakmgdllPMAKGWLINTVVDKvkkmvpayhlpqAISFKQALREGAAlS 198
Cdd:PRK06060 99 RNTEP-ALVVTSD--------------------------ALRDRFQPSRVAEA------------AELMSEAARVAPG-G 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVtlSLDDIAVLQYTGGTTGLAKGAMLTHGNLVAnMLQVLACfsqhgpdgQKLIKEGQEVMIAPLPLYHIYAFTANCM 278
Cdd:PRK06060 139 YEPM--GGDALAYATYTSGTTGPPKAAIHRHADPLT-FVDAMCR--------KALRLTPEDTGLCSARMYFAYGLGNSVW 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLitNPRDIPGFIKELGKWRF--TALLGLNTLFVALMDhpGFRQLDFSALKVTNSGGTALVKATAERW-ES 355
Cdd:PRK06060 208 FPLATGGSAVI--NSAPVTPEAAAILSARFgpSVLYGVPNFFARVID--SCSPDSFRSLRCVVSAGEALELGLAERLmEF 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LTGCRIVEGYGLTETSPVASTNPYGELaRLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATaq 435
Cdd:PRK06060 284 FGGIPILDGIGSTEVGQTFVSNRVDEW-RLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDSP-- 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 436 tLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG 515
Cdd:PRK06060 361 -VANEGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSG 439
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 820875961 516 ----GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK06060 440 atidGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
27-464 |
2.22e-31 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 128.94 E-value: 2.22e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 27 VEVFERSCKRFADRPAFS---------------------------NLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIA 79
Cdd:PTZ00216 72 LQRLERICKERGDRRALAyrpvervekevvkdadgkertmevthfNETRYITYAELWERIVNFGRGLAE-LGLTKGSNVA 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 80 VQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVyLNmfGKRVQEVL--------PDTGIEYLiea 151
Cdd:PTZ00216 151 IYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIV-CN--GKNVPNLLrlmksggmPNTTIIYL--- 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 152 kmgDLLPMAkgwlintvVDKvkkmvpayHLPQAISFKQALREGA-ALSHKPVTLSL--DDIAVLQYTGGTTGLAKGAMLT 228
Cdd:PTZ00216 225 ---DSLPAS--------VDT--------EGCRLVAWTDVVAKGHsAGSHHPLNIPEnnDDLALIMYTSGTTGDPKGVMHT 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 229 HGNLVANML----QVLACFSQHGPDgqklikegqEVMIAPLPLYHIYAFTANCMcMMVTGNHnVLITNPRDIPG-FIK-- 301
Cdd:PTZ00216 286 HGSLTAGILaledRLNDLIGPPEED---------ETYCSYLPLAHIMEFGVTNI-FLARGAL-IGFGSPRTLTDtFARph 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 302 -ELGKWRFTALLGLNTLF------------------------------VAL---MDHPGFRQLDFSA--------LKVTN 339
Cdd:PTZ00216 355 gDLTEFRPVFLIGVPRIFdtikkaveaklppvgslkrrvfdhayqsrlRALkegKDTPYWNEKVFSApravlggrVRAML 434
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 340 SGGTALVKATAErWESLTGCRIVEGYGLTETSPVASTNPYGELArLGTVGIPVADTAFKVIDDEG---NELPlGERGELC 416
Cdd:PTZ00216 435 SGGGPLSAATQE-FVNVVFGMVIQGWGLTETVCCGGIQRTGDLE-PNAVGQLLKGVEMKLLDTEEykhTDTP-EPRGEIL 511
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKK 464
Cdd:PTZ00216 512 LRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIAANGTLRIIGRVK 559
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
39-558 |
7.75e-31 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 125.21 E-value: 7.75e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQF 118
Cdd:cd17648 2 DRVAVVYGDKRLTYRELNERANRLAHYLLSVAEIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFIL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 119 KDAGARALVylnmfgkrvqevlpdTGIEylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaals 198
Cdd:cd17648 82 EDTGARVVI---------------TNST---------------------------------------------------- 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 hkpvtlsldDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSqhgpdgqkLIKEGQEVmIAPLPLYhIYAFTANCM 278
Cdd:cd17648 95 ---------DLAYAIYTSGTTGKPKGVLVEHGSVVNLRTSLSERYF--------GRDNGDEA-VLFFSNY-VFDFFVEQM 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLITNPR---DIPGFIKELGKWRFTALLGLNTLFvalmdhpgfRQLDFS---ALKVTNSGGTALVKATAER 352
Cdd:cd17648 156 TLALLNGQKLVVPPDEmrfDPDRFYAYINREKVTYLSGTPSVL---------QQYDLArlpHLKRVDAAGEEFTAPVFEK 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 353 WESLTGCRIVEGYGLTETSPVASTNPYGELARL-GTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPE 431
Cdd:cd17648 227 LRSRFAGLIINAYGPTETTVTNHKRFFPGDQRFdKSLGRPVRNTKCYVLNDAMKRVPVGAVGELYLGGDGVARGYLNRPE 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 432 ATAQTL----------DADG----WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAI-- 495
Cdd:cd17648 307 LTAERFlpnpfqteqeRARGrnarLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVak 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 496 ---GVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17648 387 edaSQAQSRIQKYLVGYYLPEPGHVPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
25-561 |
1.43e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 127.97 E-value: 1.43e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHTdlVPGDR-IAVQMPNVLQYPIAVFGALRAGLIVV 103
Cdd:PRK12467 3096 LVHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIG--VGPDVlVGVAVERSVEMIVALLAVLKAGGAYV 3173
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 104 NTNPLYTEremrhqfkdagaralvylnmfgKRVQEVLPDTGIEYLI-EAKMGDLLPMAKGwLINTVVDKvkkmvpayhlp 182
Cdd:PRK12467 3174 PLDPEYPR----------------------ERLAYMIEDSGVKLLLtQAHLLEQLPAPAG-DTALTLDR----------- 3219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 qaisfkqaLREGAALSHKPVT-LSLDDIAVLQYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQHGPDGQKLIKE----- 256
Cdd:PRK12467 3220 --------LDLNGYSENNPSTrVMGENLAYVIYTSGSTGKPKGVGVRHGAL-ANHLCWIAEAYELDANDRVLLFMsfsfd 3290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 257 -GQEVMIAPLplyhiyaftANCMCMMVTGNHNvlitnpRDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRqlDFSAL 335
Cdd:PRK12467 3291 gAQERFLWTL---------ICGGCLVVRDNDL------WDPEELWQAIHAHRISIACFPPAYLQQFAEDAGGA--DCASL 3353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 336 KVTNSGGTALVKATAERWES-LTGCRIVEGYGLTETSPVASTNPYGELARLGT----VGIPVADTAFKVIDDEGNELPLG 410
Cdd:PRK12467 3354 DIYVFGGEAVPPAAFEQVKRkLKPRGLTNGYGPTEAVVTVTLWKCGGDAVCEApyapIGRPVAGRSIYVLDGQLNPVPVG 3433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 411 ERGELCIKGPQVMKGYWQQPEATAQTLDADGWL-------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVI 483
Cdd:PRK12467 3434 VAGELYIGGVGLARGYHQRPSLTAERFVADPFSgsggrlyRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARL 3513
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 484 MGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDI 561
Cdd:PRK12467 3514 LQHPSVREAVVLARDGAGGKQLVAYVVPADPQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDP 3591
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
49-454 |
2.24e-30 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 125.76 E-value: 2.24e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 49 TLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTER-----EMRHQFkDAGA 123
Cdd:PRK08180 69 RLTYAEALERVRAIAQALLDR-GLSAERPLMILSGNSIEHALLALAAMYAGVPYAPVSPAYSLVsqdfgKLRHVL-ELLT 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 124 RALVY---LNMFGKRVQEVLPDtGIEYLIeakmgdllpmakgwlintvvdkVKKMVPAyhlPQAISFKQ--ALREGAALS 198
Cdd:PRK08180 147 PGLVFaddGAAFARALAAVVPA-DVEVVA----------------------VRGAVPG---RAATPFAAllATPPTAAVD 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 199 HKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsqhgPDgqklIKEGQEVMIAPLPLYHIYAftancm 278
Cdd:PRK08180 201 AAHAAVGPDTIAKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTF----PF----LAEEPPVLVDWLPWNHTFG------ 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 cmmvtGNHN---VL-------ITNPRDIPGFIKEL--------GKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNS 340
Cdd:PRK08180 267 -----GNHNlgiVLynggtlyIDDGKPTPGGFDETlrnlreisPTVYFNVPKGWEMLVPALERDAALRRRFFSRLKLLFY 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 341 GGTALVKATAERWESL----TGCRI--VEGYGLTETSPVAsTNPYGELARLGTVGIPVADTAFKVIddegnelPLGERGE 414
Cdd:PRK08180 342 AGAALSQDVWDRLDRVaeatCGERIrmMTGLGMTETAPSA-TFTTGPLSRAGNIGLPAPGCEVKLV-------PVGGKLE 413
|
410 420 430 440
....*....|....*....|....*....|....*....|.
gi 820875961 415 LCIKGPQVMKGYWQQPEATAQTLDADGWLKTGD-IAVIDPD 454
Cdd:PRK08180 414 VRVKGPNVTPGYWRAPELTAEAFDEEGYYRSGDaVRFVDPA 454
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
8-558 |
4.20e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 126.61 E-value: 4.20e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 8 DKRPAGVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNVLQ 87
Cdd:PRK12316 1993 DRTPEAYPRGPGVH------QRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRA-RGVGPEVRVAIAAERSFE 2065
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 88 YPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDLLPMAKGwLINT 167
Cdd:PRK12316 2066 LVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALL---------------------LTQRHLLERLPLPAG-VARL 2123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 168 VVDKVKKMvpayhlpqaisfkqalrEGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANmLQVLACFSQHG 247
Cdd:PRK12316 2124 PLDRDAEW-----------------ADYPDTAPAVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAH-CQAAGERYELS 2185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 248 PDGQKLikegQEVMIAplplyhIYAFTANCMCMMVTGNhNVLITNP--RDIPGFIKELGKWRFTALLGLNTLFVALMDH- 324
Cdd:PRK12316 2186 PADCEL----QFMSFS------FDGAHEQWFHPLLNGA-RVLIRDDelWDPEQLYDEMERHGVTILDFPPVYLQQLAEHa 2254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 325 ------PGFRQLDFsalkvtnsGGTALVKATAER-WESLTGCRIVEGYGLTETSPVAS------TNPYGelARLGTVGIP 391
Cdd:PRK12316 2255 erdgrpPAVRVYCF--------GGEAVPAASLRLaWEALRPVYLFNGYGPTEAVVTPLlwkcrpQDPCG--AAYVPIGRA 2324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 392 VADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGW-------LKTGDIAVIDPDGFTRIVDRKK 464
Cdd:PRK12316 2325 LGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRADGVVEYLGRID 2404
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 465 DMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVpDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRE 543
Cdd:PRK12316 2405 HQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVVPDDAAeDLLAELRAWLAARLPAYMVPAHWVVLE 2483
|
570
....*....|....*
gi 820875961 544 SLPMTPVGKILRREL 558
Cdd:PRK12316 2484 RLPLNPNGKLDRKAL 2498
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
45-559 |
6.67e-30 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 123.96 E-value: 6.67e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 45 NLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPL-----------YTErE 113
Cdd:PRK09192 45 QLEEALPYQTLRARAEAGARRLLAL-GLKPGDRVALIAETDGDFVEAFFACQYAGLVPV---PLplpmgfggresYIA-Q 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 114 MRHQFKDAGARALVYlnmfgkrvqevlPDTGIEYLIEAkmgdllpmakgwlintvvdkvkkmVPAYHLPQAISFKQA-LR 192
Cdd:PRK09192 120 LRGMLASAQPAAIIT------------PDELLPWVNEA------------------------THGNPLLHVLSHAWFkAL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 193 EGAALSHKPVTLslDDIAVLQYTGGTTGLAKGAMLTHGNLVANmlqvLACFSQHGPDgqklIKEGQEVmIAPLPLYH--- 269
Cdd:PRK09192 164 PEADVALPRPTP--DDIAYLQYSSGSTRFPRGVIITHRALMAN----LRAISHDGLK----VRPGDRC-VSWLPFYHdmg 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 ----IYAFTANCMC--MMVTGNHNV-------LIT-NPRDI---PGFIKE---------------LGKWRFTALLG---- 313
Cdd:PRK09192 233 lvgfLLTPVATQLSvdYLPTRDFARrplqwldLISrNRGTIsysPPFGYElcarrvnskdlaeldLSCWRVAGIGAdmir 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 314 LNTL--FVALMDHPGFRQLDF--------SALKVTNSG-GTALVKATAERwesltgcRIVEGYGLTETSPvASTNPYGEL 382
Cdd:PRK09192 313 PDVLhqFAEAFAPAGFDDKAFmpsyglaeATLAVSFSPlGSGIVVEEVDR-------DRLEYQGKAVAPG-AETRRVRTF 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 383 ARLGTvgiPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEaTAQTLDADGWLKTGDIAVIDpDGFTRIVDR 462
Cdd:PRK09192 385 VNCGK---ALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSGYFRDEE-SQDVLAADGWLDTGDLGYLL-DGYLYITGR 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 463 KKDMIIVSGFNVYPNEIEDVIMGHPKV--ANCAAIGVPDErSGEAVKLFVVAR-----EGGLSVDELKAYCKANFtGYKV 535
Cdd:PRK09192 460 AKDLIIINGRNIWPQDIEWIAEQEPELrsGDAAAFSIAQE-NGEKIVLLVQCRisdeeRRGQLIHALAALVRSEF-GVEA 537
|
570 580
....*....|....*....|....
gi 820875961 536 PKHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK09192 538 AVELVPPHSLPRTSSGKLSRAKAK 561
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
48-525 |
7.72e-30 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 123.47 E-value: 7.72e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 48 KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIvvntnPLYTEREMrhqfkdaGARALV 127
Cdd:PRK09274 40 DELSFAELDARSDAIAHGLNAA-GIGRGMRAVLMVTPSLEFFALTFALFKAGAV-----PVLVDPGM-------GIKNLK 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 128 ylnmfgKRVQEVLPDT--GIEYLIEAKMgdLLPMAKGWLINTVVdkvkkmVPAYHLPQAISFKQALREGAALSHKPVTLS 205
Cdd:PRK09274 107 ------QCLAEAQPDAfiGIPKAHLARR--LFGWGKPSVRRLVT------VGGRLLWGGTTLATLLRDGAAAPFPMADLA 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 206 LDDIAVLQYTGGTTGLAKGAMLTHGNLVAnMLQVLACFSQHGPDgqklikegqEVMIAPLPLYhiyAFTANCMcmmvtGN 285
Cdd:PRK09274 173 PDDMAAILFTSGSTGTPKGVVYTHGMFEA-QIEALREDYGIEPG---------EIDLPTFPLF---ALFGPAL-----GM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HNVL--------IT-NPRDIpgfIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAERWESL 356
Cdd:PRK09274 235 TSVIpdmdptrpATvDPAKL---FAAIERYGVTNLFGSPALLERLGRYGEANGIKLPSLRRVISAGAPVPIAVIERFRAM 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 357 --TGCRIVEGYGLTETSPVASTNPYGELARL--------GT-VGIPVADTAFKVID---------DEGNELPLGERGELC 416
Cdd:PRK09274 312 lpPDAEILTPYGATEALPISSIESREILFATraatdngaGIcVGRPVDGVEVRIIAisdapipewDDALRLATGEIGEIV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKGPQVMKGYWQQPEATAQT--LDADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANC 492
Cdd:PRK09274 392 VAGPMVTRSYYNRPEATRLAkiPDGQGdvWHRMGDLGYLDAQGRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRS 471
|
490 500 510
....*....|....*....|....*....|...
gi 820875961 493 AAIGVPdeRSGEAVKLFVVAREGGLSVDELKAY 525
Cdd:PRK09274 472 ALVGVG--VPGAQRPVLCVELEPGVACSKSALY 502
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
95-558 |
1.02e-29 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 123.19 E-value: 1.02e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 95 ALRAGLIVVNTNPLYTEreMRHQFKDAGARALVylnmfgkrVQEVLPDTGIEYLIEAKMGDLLPMAKGWLINTVVDKVKK 174
Cdd:PRK05857 41 ALRYRELVAEVGGLAAD--LRAQSVSRGSRVLV--------ISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQ 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 175 M-------------VPAYHLPQAISFKQALR-----EGAALSHKPVT--------LSLDDIAVLQYTGGTTGLAKGAMLT 228
Cdd:PRK05857 111 ItdpaaalvapgskMASSAVPEALHSIPVIAvdiaaVTRESEHSLDAaslagnadQGSEDPLAMIFTSGTTGEPKAVLLA 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 229 HGNLVANmlqvlacfsqhgPDgqKLIKEG--------QEVMIAPLPLYHIYAFTANCMCMMVTGnhnVLITNPRDIPGFI 300
Cdd:PRK05857 191 NRTFFAV------------PD--ILQKEGlnwvtwvvGETTYSPLPATHIGGLWWILTCLMHGG---LCVTGGENTTSLL 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 301 KELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAeRWESLTGCRIVEGYGLTETSPVASTNPY- 379
Cdd:PRK05857 254 EILTTNAVATTCLVPTLLSKLVSELKSANATVPSLRLVGYGGSRAIAADV-RFIEATGVRTAQVYGLSETGCTALCLPTd 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 380 -GELARL--GTVGIPVADTAFKVIDDEG------NELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAV 450
Cdd:PRK05857 333 dGSIVKIeaGAVGRPYPGVDVYLAATDGigptapGAGPSASFGTLWIKSPANMLGYWNNPERTAEVL-IDGWVNTGDLLE 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 451 IDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVARE--GGLSVDELKAYCKA 528
Cdd:PRK05857 412 RREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVASAelDESAARALKHTIAA 491
|
490 500 510
....*....|....*....|....*....|....
gi 820875961 529 NF----TGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK05857 492 RFrresEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
49-558 |
1.26e-29 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 122.20 E-value: 1.26e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 49 TLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVY 128
Cdd:cd17656 13 KLTYRELNERSNQLARFLREK-GVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVLT 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 129 LNmfgkRVQEVLPDTGIEYLIEakmGDLLPMAKGWLINTVVDKvkkmvpayhlpqaisfkqalregaalshkpvtlslDD 208
Cdd:cd17656 92 QR----HLKSKLSFNKSTILLE---DPSISQEDTSNIDYINNS-----------------------------------DD 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 209 IAVLQYTGGTTGLAKGAMLTHGNLVaNMLQvlacfsqHGPDGQKLIKEGQEVMIAPLPLYHIYA--FTANCmcmmvTGNH 286
Cdd:cd17656 130 LLYIIYTSGTTGKPKGVQLEHKNMV-NLLH-------FEREKTNINFSDKVLQFATCSFDVCYQeiFSTLL-----SGGT 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLITNP--RDIPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTAL-VKATAERWESLTGCRIVE 363
Cdd:cd17656 197 LYIIREEtkRDVEQLFDLVKRHNIEVVFLPVAFLKFIFSEREFINRFPTCVKHIITAGEQLvITNEFKEMLHEHNVHLHN 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 364 GYGLTETSPVA--STNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADG 441
Cdd:cd17656 277 HYGPSETHVVTtyTINPEAEIPELPPIGKPISNTWIYILDQEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDP 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 442 W------LKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVArEG 515
Cdd:cd17656 357 FdpnermYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLCAYFVM-EQ 435
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 820875961 516 GLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17656 436 ELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
24-558 |
1.92e-29 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 121.93 E-value: 1.92e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAV---QMPNVLqypIAVFGALRAG- 99
Cdd:PRK04813 2 MDIIETIEEFAQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFID-SLKLPDKSPIIVfghMSPEML---ATFLGAVKAGh 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 100 -LIVVNTnplYTEREmrhqfkdagaralvylnmfgkRVQEVLPDTGIEYLIEAkmGDLLPMAKGwlINTV-VDKVKKMVP 177
Cdd:PRK04813 78 aYIPVDV---SSPAE---------------------RIEMIIEVAKPSLIIAT--EELPLEILG--IPVItLDELKDIFA 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 178 AyhlPQAISFKQALREgaalshkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLV--AN-MLQVLAcfsqhgpdgqklI 254
Cdd:PRK04813 130 T---GNPYDFDHAVKG-------------DDNYYIIFTSGTTGKPKGVQISHDNLVsfTNwMLEDFA------------L 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 255 KEGQEvMIAPLPlyhiYAFTANCM----CMMVTGNHNVLitnPRDIPGFIKELgkwrFTAL--LGLNTL-----FV--AL 321
Cdd:PRK04813 182 PEGPQ-FLNQAP----YSFDLSVMdlypTLASGGTLVAL---PKDMTANFKQL----FETLpqLPINVWvstpsFAdmCL 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 322 MDhPGFRQLDFSALKVTNSGGTALVKATA----ERWESltgCRIVEGYGLTETSpVAST---------NPYGELArlgtV 388
Cdd:PRK04813 250 LD-PSFNEEHLPNLTHFLFCGEELPHKTAkkllERFPS---ATIYNTYGPTEAT-VAVTsieitdemlDQYKRLP----I 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 389 GIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQ---TLDADGWLKTGDIAVIDpDGFTRIVDRKKD 465
Cdd:PRK04813 321 GYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEaffTFDGQPAYHTGDAGYLE-DGLLFYQGRIDF 399
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 466 MIIVSGFNVYPNEIEDVIMGHPKVANCAAigVPDERSGEAVKL--FVVAREGGLSVD-----ELKAYCKANFTGYKVPKH 538
Cdd:PRK04813 400 QIKLNGYRIELEEIEQNLRQSSYVESAVV--VPYNKDHKVQYLiaYVVPKEEDFEREfeltkAIKKELKERLMEYMIPRK 477
|
570 580
....*....|....*....|
gi 820875961 539 IVLRESLPMTPVGKILRREL 558
Cdd:PRK04813 478 FIYRDSLPLTPNGKIDRKAL 497
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
6-558 |
2.60e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 123.91 E-value: 2.60e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 6 WNDKrPAGVPSTIDIHaytsvvEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQhTDLVPGDRIAVQMPNV 85
Cdd:PRK12316 3046 WNAT-AAEYPLERGVH------RLFEEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLIE-RGVGPDVLVGVAVERS 3117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 86 LQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVylnmfgkrVQEVLPdtgieylieakmgdlLPMAKGWLI 165
Cdd:PRK12316 3118 LEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLL--------SQSHLR---------------LPLAQGVQV 3174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 166 NTVvdkvkkmvpayhlpqaisfkQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLvANMLQVLACFSQ 245
Cdd:PRK12316 3175 LDL--------------------DRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSAL-SNHLCWMQQAYG 3233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 246 HGPDGQKLIKEGQEVMIAPLPLYHIYAFTANcmcmmvtgnhnVLITNPRDIPGFIKELGKWRFTALLGLNTLFVAL-MDH 324
Cdd:PRK12316 3234 LGVGDRVLQFTTFSFDVFVEELFWPLMSGAR-----------VVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLqAFL 3302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 325 PGFRQLDFSALKVTNSGGTALVKATAERWesLTGCRIVEGYGLTETS-PVASTNPYGELARLGTVGIPVADTAFKVIDDE 403
Cdd:PRK12316 3303 EEEDAHRCTSLKRIVCGGEALPADLQQQV--FAGLPLYNLYGPTEATiTVTHWQCVEEGKDAVPIGRPIANRACYILDGS 3380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 404 GNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPN 477
Cdd:PRK12316 3381 LEPVPVGALGELYLGGEGLARGYHNRPGLTAERFVPDPFVpgerlyRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELG 3460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 478 EIEDVIMGHPKVANCAAIGVPDERsgeAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRE 557
Cdd:PRK12316 3461 EIEARLLEHPWVREAVVLAVDGRQ---LVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKA 3537
|
.
gi 820875961 558 L 558
Cdd:PRK12316 3538 L 3538
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
210-501 |
3.50e-29 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 122.08 E-value: 3.50e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 210 AVLQYTGGTTGLAKGAMLTHGNLVANMlqvlACFSQHGpdGQKLIKEGQEVMIAPLPLYHIYAFTANC-MCMMVTGNhnV 288
Cdd:cd05933 153 CTLIYTSGTTGMPKGVMLSHDNITWTA----KAASQHM--DLRPATVGQESVVSYLPLSHIAAQILDIwLPIKVGGQ--V 224
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 289 LITNPRDIPG-FIKELGKWRFTALLGLNTLFVALMD-------------------------------------HPGF--- 327
Cdd:cd05933 225 YFAQPDALKGtLVKTLREVRPTAFMGVPRVWEKIQEkmkavgaksgtlkrkiaswakgvgletnlklmggespSPLFyrl 304
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 328 -RQLDFS----ALKVTN-----SGGTALVKATAERWESLTgCRIVEGYGLTETS-PVASTNPYGelARLGTVGIPVADTA 396
Cdd:cd05933 305 aKKLVFKkvrkALGLDRcqkffTGAAPISRETLEFFLSLN-IPIMELYGMSETSgPHTISNPQA--YRLLSCGKALPGCK 381
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 397 FKVI--DDEGNelplgerGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFN 473
Cdd:cd05933 382 TKIHnpDADGI-------GEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGKLDEDGFLYITGRIKELIITAgGEN 454
|
330 340
....*....|....*....|....*....
gi 820875961 474 VYPNEIEDVI-MGHPKVANCAAIGvpDER 501
Cdd:cd05933 455 VPPVPIEDAVkKELPIISNAMLIG--DKR 481
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
39-560 |
7.79e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 120.17 E-value: 7.79e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 39 DRPAFSNLGKTLTYGELDRLSAAFAAWLQqhtDLVPGDR---IAVQMPNVLQYPIAVFGALRAGLIVVNTNPlyTERemr 115
Cdd:PRK07867 18 DDRGLYFEDSFTSWREHIRGSAARAAALR---ARLDPTRpphVGVLLDNTPEFSLLLGAAALSGIVPVGLNP--TRR--- 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 116 hqfKDAGARALVYLNmfgkrVQEVLPDTGieylieakmgdLLPMAKGWLINTVVDKVKKMVPAYHLpqaisfkqALREGA 195
Cdd:PRK07867 90 ---GAALARDIAHAD-----CQLVLTESA-----------HAELLDGLDPGVRVINVDSPAWADEL--------AAHRDA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 196 ALSHKPVTLslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSqHGPDgqklikegqEVMIAPLPLYHIYAFTA 275
Cdd:PRK07867 143 EPPFRVADP--DDLFMLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFG-LGPD---------DVCYVSMPLFHSNAVMA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 276 nCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTAL--LGLNTLFV-ALMDHPGFRQldfSALKVT--NSGGTALVKATA 350
Cdd:PRK07867 211 -GWAVALAAGASIALRRKFSASGFLPDVRRYGATYAnyVGKPLSYVlATPERPDDAD---NPLRIVygNEGAPGDIARFA 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 351 ERWesltGCRIVEGYGLTET----SPVASTNPygelarlGTVGIPVADTAfkVID-DEGNELPLGER------------G 413
Cdd:PRK07867 287 RRF----GCVVVDGFGSTEGgvaiTRTPDTPP-------GALGPLPPGVA--IVDpDTGTECPPAEDadgrllnadeaiG 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 414 ELC-IKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANC 492
Cdd:PRK07867 354 ELVnTAGPGGFEGYYNDPEADAERM-RGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEV 432
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 820875961 493 AAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKA--NFTGYKVPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK07867 433 AVYAVPDPVVGDQVMAALVLAPGAkFDPDAFAEFLAAqpDLGPKQWPSYVRVCAELPRTATFKVLKRQLSA 503
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
24-559 |
1.06e-28 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 120.36 E-value: 1.06e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV- 102
Cdd:PRK08279 37 RSLGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAAR-GVGKGDVVALLMENRPEYLAAWLGLAKLGAVVa 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 -VNTN----PLyteremRHQFKDAGARALVylnmfgkrVQEVLPDTgieylIEAKMGDLLPMAKGWLINTVVDKvkkmvp 177
Cdd:PRK08279 116 lLNTQqrgaVL------AHSLNLVDAKHLI--------VGEELVEA-----FEEARADLARPPRLWVAGGDTLD------ 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 178 ayHLPQAISFKQALRegAALSHKPVT---LSLDDIAVLQYTGGTTGLAKGAMLTHGNlVANMLQVLACFSQHGPDgqkli 254
Cdd:PRK08279 171 --DPEGYEDLAAAAA--GAPTTNPASrsgVTAKDTAFYIYTSGTTGLPKAAVMSHMR-WLKAMGGFGGLLRLTPD----- 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 255 kegqEVMIAPLPLYHIYAFTAnCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTAllglntlFVA-------LMDHPGf 327
Cdd:PRK08279 241 ----DVLYCCLPLYHNTGGTV-AWSSVLAAGATLALRRKFSASRFWDDVRRYRATA-------FQYigelcryLLNQPP- 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 328 RQLDfSALKVTNSGGTALvkaTAERWESLT---GC-RIVEGYGLTEtSPVASTNPYGelaRLGTVGI--PVADTAFKVID 401
Cdd:PRK08279 308 KPTD-RDHRLRLMIGNGL---RPDIWDEFQqrfGIpRILEFYAASE-GNVGFINVFN---FDGTVGRvpLWLAHPYAIVK 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 402 -DEGNELPL------------GERGELC--IKGPQVMKGYwQQPEATAQTL------DADGWLKTGDIAVIDPDGFTRIV 460
Cdd:PRK08279 380 yDVDTGEPVrdadgrcikvkpGEVGLLIgrITDRGPFDGY-TDPEASEKKIlrdvfkKGDAWFNTGDLMRDDGFGHAQFV 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 461 DRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVP----DERSGEAVklFVVAREGGLSVDELKAYCKANFTGYKVP 536
Cdd:PRK08279 459 DRLGDTFRWKGENVATTEVENALSGFPGVEEAVVYGVEvpgtDGRAGMAA--IVLADGAEFDLAALAAHLYERLPAYAVP 536
|
570 580
....*....|....*....|...
gi 820875961 537 KHIVLRESLPMTPVGKILRRELR 559
Cdd:PRK08279 537 LFVRLVPELETTGTFKYRKVDLR 559
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
28-552 |
1.60e-28 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 120.59 E-value: 1.60e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRF-ADRPAFSNLG-KTLTYGELDRLSAAFAAWLQQHTdlVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNT 105
Cdd:PRK08043 208 EALLSAQYRYgAGKPCIEDVNfTPDSYRKLLKKTLFVGRILEKYS--VEGERIGLMLPNATISAAVIFGASLRRRIPAMM 285
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 NplYTE--REMRHQFKDAGARALVYLNMF---GK--RVQEVLPDTGIEYLIEAKmGDLLPMAKGWLIntvvdkvkkmvpa 178
Cdd:PRK08043 286 N--YTAgvKGLTSAITAAEIKTIFTSRQFldkGKlwHLPEQLTQVRWVYLEDLK-DDVTTADKLWIF------------- 349
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 179 YHLpqaisfkqALREGAALSHKPvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQV--LACFSQhgpdgqklike 256
Cdd:PRK08043 350 AHL--------LMPRLAQVKQQP-----EDAALILFTSGSEGHPKGVVHSHKSLLANVEQIktIADFTP----------- 405
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 257 gQEVMIAPLPLYHIYAFTANCMCMMVTGNHNVLITNP---RDIPgfikELGKWR-FTALLGLNTLF--VALMDHPgfrqL 330
Cdd:PRK08043 406 -NDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPlhyRIVP----ELVYDRnCTVLFGTSTFLgnYARFANP----Y 476
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 331 DFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTN-PYGelARLGTVGIPVADTAFKVIDDEGNElpl 409
Cdd:PRK08043 477 DFARLRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINvPMA--AKPGTVGRILPGMDARLLSVPGIE--- 551
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 410 gERGELCIKGPQVMKGY--------WQQPEA-TAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PRK08043 552 -QGGRLQLKGPNIMNGYlrvekpgvLEVPTAeNARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVE 630
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 481 DVIMGHPKVANCAAIGVPDERSGEAVKLFVVarEGGLSVDELKAYCKAN-FTGYKVPKHIVLRESLPMTPVGK 552
Cdd:PRK08043 631 QLALGVSPDKQHATAIKSDASKGEALVLFTT--DSELTREKLQQYAREHgVPELAVPRDIRYLKQLPLLGSGK 701
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
24-558 |
1.75e-28 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 120.92 E-value: 1.75e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 24 TSVVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVV 103
Cdd:PRK10252 458 TTLSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRER-GVKPGDSVAVALPRSVFLTLALHAIVEAGAAWL 536
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 104 NTNPLYTEREMRHQFKDAGARALVylnmfgkRVQEVLPdtgieylieakmgdllpmakgwlinTVVDKVKKMVPAYHLPQ 183
Cdd:PRK10252 537 PLDTGYPDDRLKMMLEDARPSLLI-------TTADQLP-------------------------RFADVPDLTSLCYNAPL 584
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 184 AISFKQALREGaalshKPvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANML------------QVLacfsQHGP--- 248
Cdd:PRK10252 585 APQGAAPLQLS-----QP-----HHTAYIIFTSGSTGRPKGVMVGQTAIVNRLLwmqnhypltaddVVL----QKTPcsf 650
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 249 D------------GQKLIKEGQEVMIAPLPLYHIYAFTAncmcmmVTGNHNVlitnprdiPGFIkelgkwrftallglnT 316
Cdd:PRK10252 651 DvsvweffwpfiaGAKLVMAEPEAHRDPLAMQQFFAEYG------VTTTHFV--------PSML---------------A 701
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 317 LFVALMDHPGFRQLDFSALKVTNSGgTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYG--ELARLGT----VGI 390
Cdd:PRK10252 702 AFVASLTPEGARQSCASLRQVFCSG-EALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFgeELAAVRGssvpIGY 780
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 391 PVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLDADGWL------KTGDIAVIDPDGFTRIVDRKK 464
Cdd:PRK10252 781 PVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFApgermyRTGDVARWLDDGAVEYLGRSD 860
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 465 DMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGV----PDERSGEAVKL--FVVAREG-GLSVDELKAYCKANFTGYKVPK 537
Cdd:PRK10252 861 DQLKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqAAATGGDARQLvgYLVSQSGlPLDTSALQAQLRERLPPHMVPV 940
|
570 580
....*....|....*....|.
gi 820875961 538 HIVLRESLPMTPVGKILRREL 558
Cdd:PRK10252 941 VLLQLDQLPLSANGKLDRKAL 961
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
50-454 |
2.90e-28 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 119.38 E-value: 2.90e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLqqhTD--LVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQ---------- 117
Cdd:PRK12582 81 VTYGEAKRAVDALAQAL---LDlgLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVSPAYSLMSHDHAklkhlfdlvk 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 118 ----FKDAGA---RALVYLNMFGKRVQEVLPDtgieylieAKMGDLLPMAKgWLINTVVDKVKkmvpayhlpQAIsfkqa 190
Cdd:PRK12582 158 prvvFAQSGApfaRALAALDLLDVTVVHVTGP--------GEGIASIAFAD-LAATPPTAAVA---------AAI----- 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 191 lregAALSHkpvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhGPDGqklikeGQEVMIAPLPLYHI 270
Cdd:PRK12582 215 ----AAITP-------DTVAKYLFTSGSTGMPKAVINTQRMMCANIAMQEQLRPR-EPDP------PPPVSLDWMPWNHT 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 271 YAFTANCMCMMVTGNhNVLITNPRDIPGFI-------KELGKWRF-TALLGLNTLFVALMDHPGFRQLDFSALKVTNSGG 342
Cdd:PRK12582 277 MGGNANFNGLLWGGG-TLYIDDGKPLPGMFeetirnlREISPTVYgNVPAGYAMLAEAMEKDDALRRSFFKNLRLMAYGG 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 343 TALVKATAERWESL----TGCRIV--EGYGLTETSPVaSTNPYGELARLGTVGIPVADTAFKVIddegnelPLGERGELC 416
Cdd:PRK12582 356 ATLSDDLYERMQALavrtTGHRIPfyTGYGATETAPT-TTGTHWDTERVGLIGLPLPGVELKLA-------PVGDKYEVR 427
|
410 420 430
....*....|....*....|....*....|....*....
gi 820875961 417 IKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAV-IDPD 454
Cdd:PRK12582 428 VKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAARfVDPD 466
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
28-558 |
3.54e-28 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 120.66 E-value: 3.54e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 28 EVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNP 107
Cdd:PRK05691 2192 GLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALRER-GVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDP 2270
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 108 LYTEREMRHQFKDAGARALVylnmfGKRVqevlpdtgieyLIEAkMGDLLPMAKGWLINTvvdkvkkmvpayhlpqaisf 187
Cdd:PRK05691 2271 EYPLERLHYMIEDSGIGLLL-----SDRA-----------LFEA-LGELPAGVARWCLED-------------------- 2313
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 kqalrEGAALSHKPVT----LSL-DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDGQklikegqevmi 262
Cdd:PRK05691 2314 -----DAAALAAYSDAplpfLSLpQHQAYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCE----------- 2377
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 apLPLYHIY--AFTANCMCMMVTGNHNVLIT----NPRDIPGFIKELGkwrfTALLGLNTLFVALMDHPGFRQLDFSALK 336
Cdd:PRK05691 2378 --LHFYSINfdAASERLLVPLLCGARVVLRAqgqwGAEEICQLIREQQ----VSILGFTPSYGSQLAQWLAGQGEQLPVR 2451
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALvkaTAERWESLTGC----RIVEGYGLTET--SPVASTNPygELARLGTVGIPV-----ADTAFkVIDDEGN 405
Cdd:PRK05691 2452 MCITGGEAL---TGEHLQRIRQAfapqLFFNAYGPTETvvMPLACLAP--EQLEEGAASVPIgrvvgARVAY-ILDADLA 2525
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKGPQVMKGYWQQPEATAQTLDAD------GWL-KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNE 478
Cdd:PRK05691 2526 LVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADpfaadgGRLyRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGE 2605
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 479 IEDVIMGHPKVANCAAIGVpDERSGEAVKLFVVAREGGLSVDE-------LKAYCKANFTGYKVPKHIVLRESLPMTPVG 551
Cdd:PRK05691 2606 IESRLLEHPAVREAVVLAL-DTPSGKQLAGYLVSAVAGQDDEAqaalreaLKAHLKQQLPDYMVPAHLILLDSLPLTANG 2684
|
....*..
gi 820875961 552 KILRREL 558
Cdd:PRK05691 2685 KLDRRAL 2691
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
207-558 |
2.65e-27 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 114.84 E-value: 2.65e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqkliKEGQEVMIA-PLPLYHIYAftancmcMMVTGN 285
Cdd:cd17644 106 ENLAYVIYTSGSTGKPKGVMIEHQSLVNLSHGLIKEYGITSSD-----RVLQFASIAfDVAAEEIYV-------TLLSGA 173
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HNVLITNP--RDIPGFIKELGKWRFTAL--------LGLNTLFVALMDHPgfrqldfSALKVTNSGGTALVKATAERWES 355
Cdd:cd17644 174 TLVLRPEEmrSSLEDFVQYIQQWQLTVLslppaywhLLVLELLLSTIDLP-------SSLRLVIVGGEAVQPELVRQWQK 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 356 LTGCRI--VEGYGLTETSPVAS----TNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGELCIKGPQVMKGYWQQ 429
Cdd:cd17644 247 NVGNFIqlINVYGPTEATIAATvcrlTQLTERNITSVPIGRPIANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNR 326
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 430 PEATAQTLDADGWL--------KTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDER 501
Cdd:cd17644 327 PELTAEKFISHPFNsseserlyKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQP 406
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 502 SGEAVKLFVVAR-EGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17644 407 GNKRLVAYIVPHyEESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
25-455 |
6.03e-27 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 114.91 E-value: 6.03e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 25 SVVEVFERSCKRFADRPAF--SNLGKtLTYGELDRLSAAFAAWLQQHtdlvPGDRIAVQMPNVLQYPIAVFGALRAGLIV 102
Cdd:PRK06334 20 TVLESFLKLCSEMTTATVCwdEQLGK-LSYNQVRKAVIALATKVSKY----PDQHIGIMMPASAGAYIAYFATLLSGKIP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 103 VNTNPLYTEREMRHQFKDAGARALVYLNMFGKRVQEVLPDtGIEYLIeakmgDLLPMAKgwlINTVVDKVKKMVPAYHLp 182
Cdd:PRK06334 95 VMINWSQGLREVTACANLVGVTHVLTSKQLMQHLAQTHGE-DAEYPF-----SLIYMEE---VRKELSFWEKCRIGIYM- 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 183 qAISFKQALREGAALSHKPvtlslDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSqhgpdgqkliKEGQEVMI 262
Cdd:PRK06334 165 -SIPFEWLMRWFGVSDKDP-----EDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFS----------PKEDDVMM 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 263 APLPLYHIYAFTANCMCMMVTGNHNVLITNPRDiPGFIKELGKWRFTALLGLNTLFVALMDHPGFRQ-LDFSALKVTNSG 341
Cdd:PRK06334 229 SFLPPFHAYGFNSCTLFPLLSGVPVVFAYNPLY-PKKIVEMIDEAKVTFLGSTPVFFDYILKTAKKQeSCLPSLRFVVIG 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 342 GTAL---VKATAERWESLTGCRivEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGN-ELPLGERGELCI 417
Cdd:PRK06334 308 GDAFkdsLYQEALKTFPHIQLR--QGYGTTECSPVITINTVNSPKHESCVGMPIRGMDVLIVSEETKvPVSSGETGLVLT 385
|
410 420 430
....*....|....*....|....*....|....*....
gi 820875961 418 KGPQVMKGYW-QQPEATAQTLDADGWLKTGDIAVIDPDG 455
Cdd:PRK06334 386 RGTSLFSGYLgEDFGQGFVELGGETWYVTGDLGYVDRHG 424
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
179-466 |
1.05e-26 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 114.06 E-value: 1.05e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 179 YHLPQAISFKQALREGAALSHK-P-------VTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAcFSQHGPDG 250
Cdd:cd17641 122 YDDPRLISFEDVVALGRALDRRdPglyerevAAGKGEDVAVLCTTSGTTGKPKLAMLSHGNFLGHCAAYLA-ADPLGPGD 200
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 251 QKlikegqeVMIAPLP--LYHIYA--------FTANCM----CMMV----TGNHNVLITnPR--------------DIPG 298
Cdd:cd17641 201 EY-------VSVLPLPwiGEQMYSvgqalvcgFIVNFPeepeTMMEdlreIGPTFVLLP-PRvwegiaadvrarmmDATP 272
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 299 FIKELGKW----------------------RFTALLGLNTLFVALMDHpgfrqLDFSALKVTNSGGTALVKATAERWESL 356
Cdd:cd17641 273 FKRFMFELgmklglraldrgkrgrpvslwlRLASWLADALLFRPLRDR-----LGFSRLRSAATGGAALGPDTFRFFHAI 347
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 357 tGCRIVEGYGLTETSPVASTNPYGElARLGTVGIPVADTAFKVIddegnelplgERGELCIKGPQVMKGYWQQPEATAQT 436
Cdd:cd17641 348 -GVPLKQLYGQTELAGAYTVHRDGD-VDPDTVGVPFPGTEVRID----------EVGEILVRSPGVFVGYYKNPEATAED 415
|
330 340 350
....*....|....*....|....*....|
gi 820875961 437 LDADGWLKTGDIAVIDPDGFTRIVDRKKDM 466
Cdd:cd17641 416 FDEDGWLHTGDAGYFKENGHLVVIDRAKDV 445
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
17-559 |
3.48e-26 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 112.91 E-value: 3.48e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 17 TIDIHAYTSVVEVFERSCKRFADRPAF-------SNLGKT--LTYGELDRLSAAFAAWLQQHTDlvPGDRIAVQMPNVLQ 87
Cdd:PRK12476 27 NIALPPGTTLISLIERNIANVGDTVAYryldhshSAAGCAveLTWTQLGVRLRAVGARLQQVAG--PGDRVAILAPQGID 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 88 YPIAVFGALRAGLIVVntnPLY-------TEReMRHQFKDAgaralvylnmfgkRVQEVLPDTGIEYLIEAKMGDLlpma 160
Cdd:PRK12476 105 YVAGFFAAIKAGTIAV---PLFapelpghAER-LDTALRDA-------------EPTVVLTTTAAAEAVEGFLRNL---- 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 161 kgwlintvvdkvkkmvPAYHLPQAISFkQALREGAALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVL 240
Cdd:PRK12476 164 ----------------PRLRRPRVIAI-DAIPDSAGESFVPVELDTDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMI 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 241 ACFSQHGPDGQKlikegqevmIAPLPLYHIYAFTancMCMM--VTGNHNVLITnP----RDIPGFIKELGK--------- 305
Cdd:PRK12476 227 LSIDLLDRNTHG---------VSWLPLYHDMGLS---MIGFpaVYGGHSTLMS-PtafvRRPQRWIKALSEgsrtgrvvt 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 306 ------WRFTALLGLNtlfvalmdhPGFRQLDFSAL-------KVTNSGGTALVKATAERweSLTGCRIVEGYGLTE-TS 371
Cdd:PRK12476 294 aapnfaYEWAAQRGLP---------AEGDDIDLSNVvliigsePVSIDAVTTFNKAFAPY--GLPRTAFKPSYGIAEaTL 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 372 PVASTNPYGE----------LARLGTVGIP--------------VADTAFKVI--DDEGNELPLGERGELCIKGPQVMKG 425
Cdd:PRK12476 363 FVATIAPDAEpsvvyldreqLGAGRAVRVAadapnavahvscgqVARSQWAVIvdPDTGAELPDGEVGEIWLHGDNIGRG 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 426 YWQQPEATAQTL------------DADG------WLKTGDIAVIdPDGFTRIVDRKKDMIIVSGFNVYPNEIE-DVIMGH 486
Cdd:PRK12476 443 YWGRPEETERTFgaklqsrlaegsHADGaaddgtWLRTGDLGVY-LDGELYITGRIADLIVIDGRNHYPQDIEaTVAEAS 521
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 487 PKV--ANCAAIGVPDERSGEAVklfVVA-REGGLS-------VDELKAyckANFTGYKVPKH---IVLRESLPMTPVGKI 553
Cdd:PRK12476 522 PMVrrGYVTAFTVPAEDNERLV---IVAeRAAGTSradpapaIDAIRA---AVSRRHGLAVAdvrLVPAGAIPRTTSGKL 595
|
....*.
gi 820875961 554 LRRELR 559
Cdd:PRK12476 596 ARRACR 601
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
365-562 |
1.21e-25 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 109.70 E-value: 1.21e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 365 YGLTET-SPVASTNPYGELARLGTVG--IPVAdtafKViddegnELPLGERGELCIKGPQVMKGYWQQpeataqTLDADG 441
Cdd:PRK07445 261 YGMTETaSQIATLKPDDFLAGNNSSGqvLPHA----QI------TIPANQTGNITIQAQSLALGYYPQ------ILDSQG 324
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 442 WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDE 521
Cdd:PRK07445 325 IFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDPSISLEE 404
|
170 180 190 200
....*....|....*....|....*....|....*....|.
gi 820875961 522 LKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK07445 405 LKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIA 445
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
208-470 |
2.78e-25 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 110.29 E-value: 2.78e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGpdgQKLIKEgqEVMIAPLPLYHI-------YAFTANCMCM 280
Cdd:PLN02430 221 DICTIMYTSGTSGDPKGVVLTHEAVATFVRGVDLFMEQFE---DKMTHD--DVYLSFLPLAHIldrmieeYFFRKGASVG 295
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 281 MVTGNHNVLitnpRD------------IPGFIKELGKWRFTALLGLNTL----FVALMDHP------GFRQ------LDF 332
Cdd:PLN02430 296 YYHGDLNAL----RDdlmelkptllagVPRVFERIHEGIQKALQELNPRrrliFNALYKYKlawmnrGYSHkkaspmADF 371
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 333 SAL-KVTN----------SGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVid 401
Cdd:PLN02430 372 LAFrKVKAklggrlrlliSGGAPLSTEIEEFLRVTSCAFVVQGYGLTETLGPTTLGFPDEMCMLGTVGAPAVYNELRL-- 449
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 820875961 402 DEGNEL---PLGE--RGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS 470
Cdd:PLN02430 450 EEVPEMgydPLGEppRGEICVRGKCLFSGYYKNPELTEEVM-KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLS 522
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
358-560 |
5.98e-25 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 108.58 E-value: 5.98e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 358 GCRIVEGYGLTETSPVAS---TNPYGELARlGTVGIPVADT------AFKVIDDEGNELPLGER-GELCIK-GPQVMKGY 426
Cdd:PRK13388 288 GCQVEDGYGSSEGAVIVVrepGTPPGSIGR-GAPGVAIYNPetltecAVARFDAHGALLNADEAiGELVNTaGAGFFEGY 366
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:PRK13388 367 YNNPEATAERM-RHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQV 445
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 507 KLFVVAREGG-LSVDELKAYCKANFT-GYK-VPKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PRK13388 446 MAALVLRDGAtFDPDAFAAFLAAQPDlGTKaWPRYVRIAADLPSTATNKVLKRELIA 502
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
208-559 |
1.27e-24 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 107.52 E-value: 1.27e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACfsqhgpdgQKLIKEGQEVMIAPLPLYH---------IYAFTANCM 278
Cdd:cd05915 154 AACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLV--------DGTALSEKDVVLPVVPMFHvnawclpyaATLVGAKQV 225
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 279 CMMVTGNHNVLITNprdipgfikeLGKWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALVKATAeRWESLTG 358
Cdd:cd05915 226 LPGPRLDPASLVEL----------FDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPRSLI-ARFERMG 294
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 359 CRIVEGYGLTET---------SPVASTNPYGELARL-GTVGIPVADTAFKVIDDEGNELPLGERG--ELCIKGPQVMKGY 426
Cdd:cd05915 295 VEVRQGYGLTETspvvvqnfvKSHLESLSEEEKLTLkAKTGLPIPLVRLRVADEEGRPVPKDGKAlgEVQLKGPWITGGY 374
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 427 WQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAV 506
Cdd:cd05915 375 YGNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERP 454
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 820875961 507 KLFVVAREGGLSVDELKAYCKANFTGYK-VPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05915 455 LAVVVPRGEKPTPEELNEHLLKAGFAKWqLPDAYVFAEEIPRTSAGKFLKRALR 508
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
9-505 |
1.78e-24 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 107.90 E-value: 1.78e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 9 KRPAGVPSTIDIH--AYTSVVEVFERSCKRFADRPAFS---------------------NLGKT--LTYGE-LDRLSAaF 62
Cdd:PLN02387 41 IRNARFPELVETPweGATTLAALFEQSCKKYSDKRLLGtrklisrefetssdgrkfeklHLGEYewITYGQvFERVCN-F 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 63 AAWLQQ--HtdlVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTnplyteremrhqFKDAGARALVY-LNMfgKRVQEV 139
Cdd:PLN02387 120 ASGLVAlgH---NKEERVAIFADTRAEWLIALQGCFRQNITVVTI------------YASLGEEALCHsLNE--TEVTTV 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 140 LPDTG-IEYLIEakMGDLLPMAKGWL-INTVVDKVKKMVPAYHLPQAISFKQALREGAALSHKPVTLSLDDIAVLQYTGG 217
Cdd:PLN02387 183 ICDSKqLKKLID--ISSQLETVKRVIyMDDEGVDSDSSLSGSSNWTVSSFSEVEKLGKENPVDPDLPSPNDIAVIMYTSG 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 218 TTGLAKGAMLTHGNLVANMLQVLACFSQHGPdgqklikegQEVMIAPLPLYHIYAFTA-NCMC-----------MMVTGN 285
Cdd:PLN02387 261 STGLPKGVMMTHGNIVATVAGVMTVVPKLGK---------NDVYLAYLPLAHILELAAeSVMAavgaaigygspLTLTDT 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 286 HN----------------VLITNP------RD--------IPGFIKEL-------------GKWrFTALlGLNTLFVALM 322
Cdd:PLN02387 332 SNkikkgtkgdasalkptLMTAVPaildrvRDgvrkkvdaKGGLAKKLfdiaykrrlaaieGSW-FGAW-GLEKLLWDAL 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 323 DHPGFRQLDFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELArLGTVGIPVADTAFKVID- 401
Cdd:PLN02387 410 VFKKIRAVLGGRIRFMLSGGAPLSGDTQRFINICLGAPIGQGYGLTETCAGATFSEWDDTS-VGRVGPPLPCCYVKLVSw 488
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 402 DEGNEL----PLgERGELCIKGPQVMKGYWQQPEATAQTLDADG----WLKTGDIAVIDPDGFTRIVDRKKDMI-IVSGF 472
Cdd:PLN02387 489 EEGGYLisdkPM-PRGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrWFYTGDIGQFHPDGCLEIIDRKKDIVkLQHGE 567
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 820875961 473 NVYPNEIEDVIMGHPKVAN-----------CAAIGVPDERSGEA 505
Cdd:PLN02387 568 YVSLGKVEAALSVSPYVDNimvhadpfhsyCVALVVPSQQALEK 611
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
188-562 |
3.50e-24 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 105.62 E-value: 3.50e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 KQALREGAALSHKPVTLSLDDIAVLqYTGGTTGLAKGAMLTHGNLVAnmlQVLACFSQHGpdgqklIKEGqEVMIAPLPL 267
Cdd:cd05910 67 KQCLQEAEPDAFIGIPKADEPAAIL-FTSGSTGTPKGVVYRHGTFAA---QIDALRQLYG------IRPG-EVDLATFPL 135
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 YHIYAFTANCMCMMVTGNHNVLI-TNPRDIPGFIKElgkWRFTALLGLNTLFVALMDHPGFRQLDFSALKVTNSGGTALV 346
Cdd:cd05910 136 FALFGPALGLTSVIPDMDPTRPArADPQKLVGAIRQ---YGVSIVFGSPALLERVARYCAQHGITLPSLRRVLSAGAPVP 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 347 KATAERWESLT--GCRIVEGYGLTETSPVAS---------TNPYGELARLGTVGIPVADTAFKVI--DDEG-------NE 406
Cdd:cd05910 213 IALAARLRKMLsdEAEILTPYGATEALPVSSigsrellatTTAATSGGAGTCVGRPIPGVRVRIIeiDDEPiaewddtLE 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 407 LPLGERGELCIKGPQVMKGYWQQPEATAQTLDADG----WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDV 482
Cdd:cd05910 293 LPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDNsegfWHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERV 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 483 IMGHPKVANCAAIGVpdERSGEAVKLFVVAREGGLSVD------ELKAYCKANFTGYKVPKhIVLRESLPMTPV--GKIL 554
Cdd:cd05910 373 FNTHPGVRRSALVGV--GKPGCQLPVLCVEPLPGTITPrarleqELRALAKDYPHTQRIGR-FLIHPSFPVDIRhnAKIF 449
|
....*...
gi 820875961 555 RRELRDIA 562
Cdd:cd05910 450 REKLAVWA 457
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
38-562 |
3.06e-23 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 103.88 E-value: 3.06e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAF------SNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI--VV----NT 105
Cdd:PRK10524 67 PEQLALiavsteTDEERTYTFRQLHDEVNRMAAMLR-SLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIhsVVfggfAS 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 NPLYTEREmrhqfkDAGARALVYLNMfGKRVQEVLP-----DTGIEyLIEAKMGDLLpmakgwLINTVVDKvKKMVPAYH 180
Cdd:PRK10524 146 HSLAARID------DAKPVLIVSADA-GSRGGKVVPykpllDEAIA-LAQHKPRHVL------LVDRGLAP-MARVAGRD 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 181 LPQAisfkqALREGAALSHKPVT-LSLDDIAVLQYTGGTTGLAKGAML-THGNLVAnmlqvLACFSQHGPDGqkliKEGq 258
Cdd:PRK10524 211 VDYA-----TLRAQHLGARVPVEwLESNEPSYILYTSGTTGKPKGVQRdTGGYAVA-----LATSMDTIFGG----KAG- 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 259 EVMIAPLPL-------YHIYA-FTANCMCMMVTGnhnvLITNPRdiPGFIkelgkWRFTALLGLNTLFVA------LMDH 324
Cdd:PRK10524 276 ETFFCASDIgwvvghsYIVYApLLAGMATIMYEG----LPTRPD--AGIW-----WRIVEKYKVNRMFSAptairvLKKQ 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 325 PG--FRQLDFSALKVTNSGGTALVKATAeRW--ESLtGCRIVEGYGLTETS-PVASTNPYGELA--RLGTVGIPVADTAF 397
Cdd:PRK10524 345 DPalLRKHDLSSLRALFLAGEPLDEPTA-SWisEAL-GVPVIDNYWQTETGwPILAIARGVEDRptRLGSPGVPMYGYNV 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 398 KVIDDE-GNELPLGERGELCIKGP---QVMKGYWQQPEATAQTLdadgW-------LKTGDIAVIDPDGFTRIVDRKKDM 466
Cdd:PRK10524 423 KLLNEVtGEPCGPNEKGVLVIEGPlppGCMQTVWGDDDRFVKTY----WslfgrqvYSTFDWGIRDADGYYFILGRTDDV 498
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 467 IIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVD---------ELKAYCKANFTGYKVPK 537
Cdd:PRK10524 499 INVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADrearlalekEIMALVDSQLGAVARPA 578
|
570 580
....*....|....*....|....*
gi 820875961 538 HIVLRESLPMTPVGKILRRELRDIA 562
Cdd:PRK10524 579 RVWFVSALPKTRSGKLLRRAIQAIA 603
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
398-562 |
1.19e-22 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 102.14 E-value: 1.19e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 398 KVIDDEGNELPLGERGELCIKG--PQVMKGYWQQPEATAQTL--DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFN 473
Cdd:PRK00174 436 AVVDEEGNPLEGGEGGNLVIKDpwPGMMRTIYGDHERFVKTYfsTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHR 515
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 474 VYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREG-----GLsVDELKAYCKANFTGYKVPKHIVLRESLPMT 548
Cdd:PRK00174 516 LGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGeepsdEL-RKELRNWVRKEIGPIAKPDVIQFAPGLPKT 594
|
170
....*....|....
gi 820875961 549 PVGKILRRELRDIA 562
Cdd:PRK00174 595 RSGKIMRRILRKIA 608
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
26-558 |
3.34e-22 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 101.78 E-value: 3.34e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 26 VVEVFERSCKRFADRPAFSNLGKTLTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNT 105
Cdd:PRK05691 1133 LPELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLR-DKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPL 1211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 106 NPLYTEREMRHQFKDAGARALvylnmfgkrvqevlpdtgieyLIEAKMGDLLPMAKGwlintvvdkvkkmVPAYHLPQai 185
Cdd:PRK05691 1212 DPDYPAERLAYMLADSGVELL---------------------LTQSHLLERLPQAEG-------------VSAIALDS-- 1255
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 186 sfkqaLREGAALSHKP-VTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAP 264
Cdd:PRK05691 1256 -----LHLDSWPSQAPgLHLHGDNLAYVIYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSD----------VLMQK 1320
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 265 LPLyhiyAFTAN---CMCMMVTGNHNVLIT-----NPRDIPGFIKELGkwrFTALLGLNTLFVALMDHPGFRQLdfSALK 336
Cdd:PRK05691 1321 API----SFDVSvweCFWPLITGCRLVLAGpgehrDPQRIAELVQQYG---VTTLHFVPPLLQLFIDEPLAAAC--TSLR 1391
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 VTNSGGTALVKATAER-WESLTGCRIVEGYGLTETS-PVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGE 414
Cdd:PRK05691 1392 RLFSGGEALPAELRNRvLQRLPQVQLHNRYGPTETAiNVTHWQCQAEDGERSPIGRPLGNVLCRVLDAELNLLPPGVAGE 1471
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 415 LCIKGPQVMKGYWQQPEATAQTL--DADG-----WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHP 487
Cdd:PRK05691 1472 LCIGGAGLARGYLGRPALTAERFvpDPLGedgarLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQP 1551
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 488 KVANcAAIGVPDERSG-EAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK05691 1552 GVAQ-AAVLVREGAAGaQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRAL 1622
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
478-552 |
5.50e-22 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 89.91 E-value: 5.50e-22
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 820875961 478 EIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGG-LSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGK 552
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVeLLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
445-558 |
8.08e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 97.80 E-value: 8.08e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 445 TGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREgGLSVDELKA 524
Cdd:PRK08308 295 TKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHE-EIDPVQLRE 373
|
90 100 110
....*....|....*....|....*....|....
gi 820875961 525 YCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK08308 374 WCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
288-559 |
5.43e-21 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 95.71 E-value: 5.43e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHP--GFRqldfSALKVTNSGGTALVKATAERWESLTGCRIVEG 364
Cdd:cd05974 156 FLFNYARfDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDlaSFD----VKLREVVGAGEPLNPEVIEQVRRAWGLTIRDG 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 365 YGLTETSPVASTNPyGELARLGTVGIPVADTAFKVIDDEGNElplGERGELCI-----KGPQVMKGYWQQPEATAQTLdA 439
Cdd:cd05974 232 YGQTETTALVGNSP-GQPVKAGSMGRPLPGYRVALLDPDGAP---ATEGEVALdlgdtRPVGLMKGYAGDPDKTAHAM-R 306
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 440 DGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSV 519
Cdd:cd05974 307 GGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPS 386
|
250 260 270 280
....*....|....*....|....*....|....*....|....
gi 820875961 520 DELK----AYCKANFTGYKVPKHIVLREsLPMTPVGKILRRELR 559
Cdd:cd05974 387 PETAleifRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELR 429
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
47-559 |
1.08e-20 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 94.73 E-value: 1.08e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIV--VNTNplYTEREMRHQFKDAGAR 124
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSL-GLKPGDVVALFMENRPEYVLLWLGLVKIGAVAalINYN--LRGESLAHCLNVSSAK 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 125 ALVYlnmfgkrvqevlpdtgieylieakmgdllpmakgwlintvvdkvkkmvpayhlpqaisfkqalregaalshkpvtl 204
Cdd:cd05940 78 HLVV---------------------------------------------------------------------------- 81
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 sldDIAVLQYTGGTTGLAKGAMLTHGNLVANMLqvlacFSQHGpdgqkLIKEGQEVMIAPLPLYHIYAFTAnCMCMMVTG 284
Cdd:cd05940 82 ---DAALYIYTSGTTGLPKAAIISHRRAWRGGA-----FFAGS-----GGALPSDVLYTCLPLYHSTALIV-GWSACLAS 147
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 285 NHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALKVTNSGGTALvkaTAERWESLTG----CR 360
Cdd:cd05940 148 GATLVIRKKFSASNFWDDIRKYQATIFQYIGELCRYLLNQP--PKPTERKHKVRMIFGNGL---RPDIWEEFKErfgvPR 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 361 IVEGYGLTE--TSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNEL-----------PLGERGELC--IKGPQVMKG 425
Cdd:cd05940 223 IAEFYAATEgnSGFINFFGKPGAIGRNPSLLRKVAPLALVKYDLESGEPirdaegrcikvPRGEPGLLIsrINPLEPFDG 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 426 YWQQPEATAQTL-----DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAIGVP-- 498
Cdd:cd05940 303 YTDPAATEKKILrdvfkKGDAWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQvp 382
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 820875961 499 --DERSGEAVklFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05940 383 gtDGRAGMAA--IVLQPNEEFDLSALAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLR 443
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
208-482 |
1.31e-19 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 92.78 E-value: 1.31e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFsqhGPDGQKLIKegQEVMIAPLPLYHIYAFTANcMCMMvtgNHN 287
Cdd:PLN02614 224 DICTIMYTSGTTGDPKGVMISNESIVTLIAGVIRLL---KSANAALTV--KDVYLSYLPLAHIFDRVIE-ECFI---QHG 294
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 288 VLITNPR-DIPGFIKELGKWRFTALLG----LNTLFVALMDH---------------------------------PGFRQ 329
Cdd:PLN02614 295 AAIGFWRgDVKLLIEDLGELKPTIFCAvprvLDRVYSGLQKKlsdggflkkfvfdsafsykfgnmkkgqshveasPLCDK 374
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 330 LDFSALK--------VTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFK--- 398
Cdd:PLN02614 375 LVFNKVKqglggnvrIILSGAAPLASHVESFLRVVACCHVLQGYGLTESCAGTFVSLPDELDMLGTVGPPVPNVDIRles 454
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 399 VIDDEGNELPLGERGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-GFNVYPN 477
Cdd:PLN02614 455 VPEMEYDALASTPRGEICIRGKTLFSGYYKREDLTKEVL-IDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLSqGEYVAVE 533
|
....*
gi 820875961 478 EIEDV 482
Cdd:PLN02614 534 NIENI 538
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
47-548 |
7.18e-19 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 90.04 E-value: 7.18e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARAL 126
Cdd:cd05938 3 GETYTYRDVDRRSNQAARALLAHAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKVL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 VYLNMFGKRVQEVLP---DTGIEylIEAKMGDLLPMAKGWLINTVVDKVKKMVPAYhLPQAISFKqalregaalshkpvt 203
Cdd:cd05938 83 VVAPELQEAVEEVLPalrADGVS--VWYLSHTSNTEGVISLLDKVDAASDEPVPAS-LRAHVTIK--------------- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 204 lsldDIAVLQYTGGTTGLAKGAMLTHGNLVA--NMLQVLACFSqhgpdgqklikegQEVMIAPLPLYHIYAFTANCMCMM 281
Cdd:cd05938 145 ----SPALYIYTSGTTGLPKAARISHLRVLQcsGFLSLCGVTA-------------DDVIYITLPLYHSSGFLLGIGGCI 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 VTGNHNVLitNPR-DIPGFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALKVTNSGGTALvkaTAERWESLT--- 357
Cdd:cd05938 208 ELGATCVL--KPKfSASQFWDDCRKHNVTVIQYIGELLRYLCNQP--QSPNDRDHKVRLAIGNGL---RADVWREFLrrf 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 358 -GCRIVEGYGLTETSpVASTNPYGELARLGTVG------IPVA------DTAFKVIDDEGN--ELPLGERGELCIKGPQV 422
Cdd:cd05938 281 gPIRIREFYGSTEGN-IGFFNYTGKIGAVGRVSylykllFPFElikfdvEKEEPVRDAQGFciPVAKGEPGLLVAKITQQ 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 423 --MKGYWQQPEATAQTL------DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKV--ANC 492
Cdd:cd05938 360 spFLGYAGDKEQTEKKLlrdvfkKGDVYFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLLDFLqeVNV 439
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 493 AAIGVPDE--RSG-EAVKLfvvaREG-GLSVDELKAYCKANFTGYKVPKHIVLRESLPMT 548
Cdd:cd05938 440 YGVTVPGHegRIGmAAVKL----KPGhEFDGKKLYQHVREYLPAYARPRFLRIQDSLEIT 495
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
12-549 |
1.68e-18 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 89.05 E-value: 1.68e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 12 AGVPS---TIDIHAYT-SVVEVFERSCKRFADRPAFS----------NLGK----------TLTYGELDRLSAAFAAWLQ 67
Cdd:cd17632 6 AAAPLeavTEAIRRPGlRLAQIIATVMTGYADRPALGqratelvtdpATGRttlrllprfeTITYAELWERVGAVAAAHD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 68 QHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLyteremrhqfkDAGARAlvylnmfgKRVQEVLPDTGIEY 147
Cdd:cd17632 86 PEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSV---PL-----------QAGASA--------AQLAPILAETEPRL 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 148 LieAKMGDLLPMAkgwlINTVVD--KVKKMVPAYHLPQAISFKQAL---REGAALSHKPVTL------------------ 204
Cdd:cd17632 144 L--AVSAEHLDLA----VEAVLEggTPPRLVVFDHRPEVDAHRAALesaRERLAAVGIPVTTltliavrgrdlppaplfr 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 ---SLDDIAVLQYTGGTTGLAKGAMLTHgNLVANMLQVLacfsqhgpDGQKLIKEGQEVMIAPLPLYHIYAftancmcmm 281
Cdd:cd17632 218 pepDDDPLALLIYTSGSTGTPKGAMYTE-RLVATFWLKV--------SSIQDIRPPASITLNFMPMSHIAG--------- 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 vtgnHNVLITN-----------PRDIPGFIKELGKWRFTALL-------GLNTLFVALMDHPGFRQLDFSALK------- 336
Cdd:cd17632 280 ----RISLYGTlarggtayfaaASDMSTLFDDLALVRPTELFlvprvcdMLFQRYQAELDRRSVAGADAETLAervkael 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 337 ---------VTNSGGTALVKATAERW-ESLTGCRIVEGYGLTETSPVASTnpyGELARlgtvgIPVADtaFKVIDdegne 406
Cdd:cd17632 356 rervlggrlLAAVCGSAPLSAEMKAFmESLLDLDLHDGYGSTEAGAVILD---GVIVR-----PPVLD--YKLVD----- 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 407 LP-LG--------ERGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDI-AVIDPDGFTrIVDRKKDMIIVS-GFNVY 475
Cdd:cd17632 421 VPeLGyfrtdrphPRGELLVKTDTLFPGYYKRPEVTAEVFDEDGFYRTGDVmAELGPDRLV-YVDRRNNVLKLSqGEFVT 499
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 476 PNEIEDVIMGHPKV------ANCA-----AIGVPDERSGEAVKlfvVAREGGLSVDELKAYCK-ANFTGYKVPKHIVLrE 543
Cdd:cd17632 500 VARLEAVFAASPLVrqifvyGNSErayllAVVVPTQDALAGED---TARLRAALAESLQRIAReAGLQSYEIPRDFLI-E 575
|
....*.
gi 820875961 544 SLPMTP 549
Cdd:cd17632 576 TEPFTI 581
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
207-504 |
3.03e-18 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 88.36 E-value: 3.03e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVlacfsqhgpdgQKLIKEGQEVM------IAPLPLYHIYAFTANCMCM 280
Cdd:PLN02861 220 TDICTIMYTSGTTGEPKGVILTNRAIIAEVLST-----------DHLLKVTDRVAteedsyFSYLPLAHVYDQVIETYCI 288
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 281 -------MVTGNHNVLITN------------PR-------------DIPGFI-KELGKWRFTALLG-LNTLFVALMDHPG 326
Cdd:PLN02861 289 skgasigFWQGDIRYLMEDvqalkptifcgvPRvydriytgimqkiSSGGMLrKKLFDFAYNYKLGnLRKGLKQEEASPR 368
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 327 FRQLDFSALKVTNSG-------GTALVKATAERWESLTGCR-IVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFK 398
Cdd:PLN02861 369 LDRLVFDKIKEGLGGrvrlllsGAAPLPRHVEEFLRVTSCSvLSQGYGLTESCGGCFTSIANVFSMVGTVGVPMTTIEAR 448
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 399 VID------DEGNELPlgeRGELCIKGPQVMKGYWQQPEATAQTLdADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS-G 471
Cdd:PLN02861 449 LESvpemgyDALSDVP---RGEICLRGNTLFSGYHKRQDLTEEVL-IDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSqG 524
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 820875961 472 FNVYPNEIEDVIMGHPKVANC-----------AAIGVPDERSGE 504
Cdd:PLN02861 525 EYVAVENLENTYSRCPLIASIwvygnsfesflVAVVVPDRQALE 568
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
191-558 |
8.89e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 87.92 E-value: 8.89e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 191 LREGAALSHKPVTLSL-DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQhgpDGQKLIKE--GQEVMIAplpl 267
Cdd:PRK05691 3852 VQAGEVASHNPGIYSGpDNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLAL---SEADVIAQtaSQSFDIS---- 3924
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 268 yhIYAFTANCMcmmvTGNHNVLITN--PRDIPGFIKELGKWRFTALLGLNTLFVALMDHPgfrQLDFSALKVTNSGGTAL 345
Cdd:PRK05691 3925 --VWQFLAAPL----FGARVEIVPNaiAHDPQGLLAHVQAQGITVLESVPSLIQGMLAED---RQALDGLRWMLPTGEAM 3995
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 346 VKATAERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLGT---VGIPVADTAFKVIDDEGNELPLGERGELCIKGPQ 421
Cdd:PRK05691 3996 PPELARQWlQRYPQIGLVNAYGPAECSDDVAFFRVDLASTRGSylpIGSPTDNNRLYLLDEALELVPLGAVGELCVAGTG 4075
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 422 VMKGYWQQPEATAQTL-----DADG--WLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANcAA 494
Cdd:PRK05691 4076 VGRGYVGDPLRTALAFvphpfGAPGerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVRE-AA 4154
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 495 IGVPDERSGEAVKLFVVAREGGLS----VDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:PRK05691 4155 VAVQEGVNGKHLVGYLVPHQTVLAqgalLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
331-555 |
2.27e-17 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 85.56 E-value: 2.27e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 331 DFSALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSpVASTNPYGELAR-LGTVGIPVADTAFKVIDDEGNELPL 409
Cdd:PTZ00237 378 DLSNLKEIWCGGEVIEESIPEYIENKLKIKSSRGYGQTEIG-ITYLYCYGHINIpYNATGVPSIFIKPSILSEDGKELNV 456
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 410 GERGELCIK---GPQVMKGYWQQPEATAQTLDA-DGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMG 485
Cdd:PTZ00237 457 NEIGEVAFKlpmPPSFATTFYKNDEKFKQLFSKfPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILK 536
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 820875961 486 HPKVANCAAIGV--PDERSGEAVKLFVVAREGGLSVD------ELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILR 555
Cdd:PTZ00237 537 HPLVLECCSIGIydPDCYNVPIGLLVLKQDQSNQSIDlnklknEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPR 614
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
194-560 |
5.58e-15 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 77.47 E-value: 5.58e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 194 GAALSH-------KPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLP 266
Cdd:cd05937 67 GDPLIHclklsgsRFVIVDPDDPAILIYTSGTTGLPKAAAISWRRTLVTSNLLSHDLNLKNGD----------RTYTCMP 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 LYHIYAFTAnCMCMMVTGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALKVTNSGGTALV 346
Cdd:cd05937 137 LYHGTAAFL-GACNCLMSGGTLALSRKFSASQFWKDVRDSGATIIQYVGELCRYLLSTP--PSPYDRDHKVRVAWGNGLR 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 347 KATAERWESLTGCRIV-EGYGLTEtSPVASTN------------PYGELAR--LGTVGIPVA---DTAFKVIDDEGN--- 405
Cdd:cd05937 214 PDIWERFRERFNVPEIgEFYAATE-GVFALTNhnvgdfgagaigHHGLIRRwkFENQVVLVKmdpETDDPIRDPKTGfcv 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 406 ELPLGERGELCIKGPQVMK----GYWQQPEATAQTL------DADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVY 475
Cdd:cd05937 293 RAPVGEPGEMLGRVPFKNReafqGYLHNEDATESKLvrdvfrKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVS 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 476 PNEIEDVIMGHPKV--ANCAAIGVP--DERSGEAVklfVVAREGGLSVDE-----LKAYCKANFTGYKVPKHIVLRESLP 546
Cdd:cd05937 373 TTEVADVLGAHPDIaeANVYGVKVPghDGRAGCAA---ITLEESSAVPTEftkslLASLARKNLPSYAVPLFLRLTEEVA 449
|
410
....*....|....
gi 820875961 547 MTPVGKILRRELRD 560
Cdd:cd05937 450 TTDNHKQQKGVLRD 463
|
|
| Dip2 |
cd05905 |
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of ... |
47-560 |
1.22e-14 |
|
Disco-interacting protein 2 (Dip2); Dip2 proteins show sequence similarity to other members of the adenylate forming enzyme family, including insect luciferase, acetyl CoA ligases and the adenylation domain of nonribosomal peptide synthetases (NRPS). However, its function may have diverged from other members of the superfamily. In mouse embryo, Dip2 homolog A plays an important role in the development of both vertebrate and invertebrate nervous systems. Dip2A appears to regulate cell growth and the arrangement of cells in organs. Biochemically, Dip2A functions as a receptor of FSTL1, an extracellular glycoprotein, and may play a role as a cardiovascular protective agent.
Pssm-ID: 341231 [Multi-domain] Cd Length: 571 Bit Score: 76.62 E-value: 1.22e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLyterEMRHQFKDAGAral 126
Cdd:cd05905 12 ATTLTWGKLLSRAEKIAAVLQKKVGLKPGDRVALMYPDPLDFVAAFYGCLYAGVVPI---PI----EPPDISQQLGF--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 127 vYLNMFG----KRVQEVLPDTgIEYLIEAKMGDLLPMAKGWLINTVVDKVKKMVPAYHLPQAIsfkqalregaalsHKPv 202
Cdd:cd05905 82 -LLGTCKvrvaLTVEACLKGL-PKKLLKSKTAAEIAKKKGWPKILDFVKIPKSKRSKLKKWGP-------------HPP- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 203 tLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMlQVLACFSQHGPDgqklikegqEVMIAPLPLYHIYAFTANCMCMMV 282
Cdd:cd05905 146 -TRDGDTAYIEYSFSSDGSLSGVAVSHSSLLAHC-RALKEACELYES---------RPLVTVLDFKSGLGLWHGCLLSVY 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 283 TGNHNVLI------TNPrdiPGFIKELGKWR-FTALLGLNTLFVALMDHPgfRQLDFSALK-VTNSGGTALVKATAERWE 354
Cdd:cd05905 215 SGHHTILIppelmkTNP---LLWLQTLSQYKvRDAYVKLRTLHWCLKDLS--STLASLKNRdVNLSSLRMCMVPCENRPR 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 355 SLTGCRIVEGY---GLTET--SPVASTNPYGELARLGTVGIPVA-----------DTAFKVIDDEGNELPL--------- 409
Cdd:cd05905 290 ISSCDSFLKLFqtlGLSPRavSTEFGTRVNPFICWQGTSGPEPSrvyldmralrhGVVRLDERDKPNSLPLqdsgkvlpg 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 410 ----------------GERGELCIKGPQVMKGYWQQPEATAQTLDA------------DGWLKTGDIAVIDPDGFTR--- 458
Cdd:cd05905 370 aqvaivnpetkglckdGEIGEIWVNSPANASGYFLLDGETNDTFKVfpstrlstgitnNSYARTGLLGFLRPTKCTDlnv 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 459 -------IVDRKKDMIIVSGFNVYPNEIEDVIMG-HPKVANCAAIgvpdeRSGEAVKLFVVAREGG-------------- 516
Cdd:cd05905 450 eehdllfVVGSIDETLEVRGLRHHPSDIEATVMRvHPYRGRCAVF-----SITGLVVVVAEQPPGSeeealdlvplvlna 524
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 820875961 517 -LSVDELKAYCKAnftgykvpkhIVLRESLPMTPVGKILRRELRD 560
Cdd:cd05905 525 iLEEHQVIVDCVA----------LVPPGSLPKNPLGEKQRMEIRQ 559
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
334-562 |
1.92e-14 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 76.47 E-value: 1.92e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 334 ALKVTNSGGTAlVKATAERW-ESLTG---CRIVEGYGLTET-----SPVASTNPygelARLGTVGIPVADTAFKVIDDEG 404
Cdd:PLN02654 398 SLRVLGSVGEP-INPSAWRWfFNVVGdsrCPISDTWWQTETggfmiTPLPGAWP----QKPGSATFPFFGVQPVIVDEKG 472
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 405 NELPLGERGELCIKG--PQVMKGYWQQPEATAQTLDA--DGWLKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIE 480
Cdd:PLN02654 473 KEIEGECSGYLCVKKswPGAFRTLYGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVE 552
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 481 DVIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDELKA----YCKANFTGYKVPKHIVLRESLPMTPVGKILRR 556
Cdd:PLN02654 553 SALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKslilTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 632
|
....*.
gi 820875961 557 ELRDIA 562
Cdd:PLN02654 633 ILRKIA 638
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
207-470 |
2.43e-13 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 72.83 E-value: 2.43e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 207 DDIAVLQYTGGTTGLAKGAMLTHGNLVaNMLqVLACfsqhgpDGQKLIKEGQEVMIAPLPLYHIYAFTANCMCMMVTGNH 286
Cdd:PTZ00342 304 DFITSIVYTSGTSGKPKGVMLSNKNLY-NTV-VPLC------KHSIFKKYNPKTHLSYLPISHIYERVIAYLSFMLGGTI 375
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 287 NVLitnPRDIPGFIKELGKWRFTALLGL----NTLFVALM------------------------DHPGFRQL-----DFS 333
Cdd:PTZ00342 376 NIW---SKDINYFSKDIYNSKGNILAGVpkvfNRIYTNIMteinnlpplkrflvkkilslrksnNNGGFSKFlegitHIS 452
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 334 A---------LKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETS-PVASTNPYGElaRLGTVGIPVA-DTAFKVIDD 402
Cdd:PTZ00342 453 SkikdkvnpnLEVILNGGGKLSPKIAEELSVLLNVNYYQGYGLTETTgPIFVQHADDN--NTESIGGPISpNTKYKVRTW 530
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 820875961 403 E----GNELPlgeRGELCIKGPQVMKGYWQQPEATAQTLDADGWLKTGDIAVIDPDGFTRIVDRKKDMIIVS 470
Cdd:PTZ00342 531 EtykaTDTLP---KGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGDIVQINKNGSLTFLDRSKGLVKLS 599
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
195-559 |
2.69e-13 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 72.49 E-value: 2.69e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 195 AALSHKPVTLSL-----DDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSqhgpdgqklIKEGQEVMIAPLPLYH 269
Cdd:PRK05851 135 ATAAHTNRSASLtppdsGGPAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVG---------LDAATDVGCSWLPLYH 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 270 IYAFTancmcmmvtgnhnVLITnprdipGFIKELGKWR-----FTA-----LLGLNTLFVALMDHPGF------------ 327
Cdd:PRK05851 206 DMGLA-------------FLLT------AALAGAPLWLapttaFSAspfrwLSWLSDSRATLTAAPNFaynligkyarrv 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 328 RQLDFSALKVTNSGGTALVKATAERWES------LTGCRIVEGYGLTE-----TSPV---------ASTNPYGELARLGT 387
Cdd:PRK05851 267 SDVDLGALRVALNGGEPVDCDGFERFATamapfgFDAGAAAPSYGLAEstcavTVPVpgiglrvdeVTTDDGSGARRHAV 346
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 388 VGIPVADTAFKVIDDEGNElPLGER--GELCIKGPQVMKGYWQQpeataQTLDADGWLKTGDIAVIDPDGFTrIVDRKKD 465
Cdd:PRK05851 347 LGNPIPGMEVRISPGDGAA-GVAGReiGEIEIRGASMMSGYLGQ-----APIDPDDWFPTGDLGYLVDGGLV-VCGRAKE 419
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 466 MIIVSGFNVYPNEIEDVIMGHPKVANCAAIGV-PDE---RSGEAVKL-FVVAREGGLS---VDELKAYCKAnftgykVPK 537
Cdd:PRK05851 420 LITVAGRNIFPTEIERVAAQVRGVREGAVVAVgTGEgsaRPGLVIAAeFRGPDEAGARsevVQRVASECGV------VPS 493
|
410 420
....*....|....*....|....
gi 820875961 538 HIVLRE--SLPMTPVGKILRRELR 559
Cdd:PRK05851 494 DVVFVApgSLPRTSSGKLRRLAVK 517
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
36-536 |
2.93e-13 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 71.83 E-value: 2.93e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 36 RFADRPAFSNLGKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPlyteremr 115
Cdd:PRK09029 15 VRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQ-GVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNP-------- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 116 hQFKDAgaralvylnmfgkRVQEVLPDTGIEYLIEAKMGDllpmakgwlintvvdkvkkmvpayhlpqAISFKQALREGA 195
Cdd:PRK09029 86 -QLPQP-------------LLEELLPSLTLDFALVLEGEN----------------------------TFSALTSLHLQL 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 196 ALSHKPVTLSLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLAC--FSQHgpDGQKLikegqevmiaPLPLYHiyaf 273
Cdd:PRK09029 124 VEGAHAVAWQPQRLATMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLmpFTAQ--DSWLL----------SLPLFH---- 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 274 tancmcmmVTGNhnvlitnprdipGFIkelgkWRFT---ALLGLNT---LFVALMD--HPGF------RQLDFSALKVTN 339
Cdd:PRK09029 188 --------VSGQ------------GIV-----WRWLyagATLVVRDkqpLEQALAGctHASLvptqlwRLLDNRSEPLSL 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 340 S----GGTALVKATAERWESLtGCRIVEGYGLTEtspVASTNPYGELARLGTVGIPVADTAFKVIDDEgnelplgergeL 415
Cdd:PRK09029 243 KavllGGAAIPVELTEQAEQQ-GIRCWCGYGLTE---MASTVCAKRADGLAGVGSPLPGREVKLVDGE-----------I 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 416 CIKGPQVMKGYWQQPEATAQTlDADGWLKTGDIAVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCAAI 495
Cdd:PRK09029 308 WLRGASLALGYWRQGQLVPLV-NDEGWFATRDRGEWQ-NGELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVV 385
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 820875961 496 GVPDERSGE---AvklfVVAREGGLSVDELKAYCKANFTGYKVP 536
Cdd:PRK09029 386 PVADAEFGQrpvA----VVESDSEAAVVNLAEWLQDKLARFQQP 425
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
350-515 |
6.28e-13 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 70.56 E-value: 6.28e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 350 AERW--------ESLTGCRIVEGYGLTETSP-VASTNPYGElarlgtvGIPVADTAF--KVIDDEGNE-LPLGERGELCI 417
Cdd:COG1541 212 GEPWseemrkeiEERWGIKAYDIYGLTEVGPgVAYECEAQD-------GLHIWEDHFlvEIIDPETGEpVPEGEEGELVV 284
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 418 kgpqvmkgywqqpeaTaqTLDADGW----LKTGDIAVIDPD----GFT-----RIVDRKKDMIIVSGFNVYPNEIEDVIM 484
Cdd:COG1541 285 ---------------T--TLTKEAMplirYRTGDLTRLLPEpcpcGRThprigRILGRADDMLIIRGVNVFPSQIEEVLL 347
|
170 180 190
....*....|....*....|....*....|.
gi 820875961 485 GHPKVANCAAIGVPDERSGEAVKLFVVAREG 515
Cdd:COG1541 348 RIPEVGPEYQIVVDREGGLDELTVRVELAPG 378
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
50-561 |
3.49e-11 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 65.76 E-value: 3.49e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 50 LTYGELDRLSAAFAAWLQqHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTEREMRHQFKDAGARALVYL 129
Cdd:cd05943 99 VTWAELRRRVARLAAALR-ALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQIEPKVLFAV 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 130 N--MFGKRVQEVLPDtgIEYLIEAkmgdLLPMAKGWLINTVVDKVKKMVPayHLPQAISFKQALREGAALSHKPVTLSLD 207
Cdd:cd05943 178 DayTYNGKRHDVREK--VAELVKG----LPSLLAVVVVPYTVAAGQPDLS--KIAKALTLEDFLATGAAGELEFEPLPFD 249
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 208 DIAVLQYTGGTTGLAKGAMLTHGNLVanmLQVLACFSQHGPdgqklIKEGQEVMiaplplyhiyaFTANCMCMM------ 281
Cdd:cd05943 250 HPLYILYSSGTTGLPKCIVHGAGGTL---LQHLKEHILHCD-----LRPGDRLF-----------YYTTCGWMMwnwlvs 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 282 --VTGNHNVLITNPrdiPGFIKELGKWRFTALLGLNTL------FVALMD---HPGfRQLDFSALKVTNSGGTALVKATA 350
Cdd:cd05943 311 glAVGATIVLYDGS---PFYPDTNALWDLADEEGITVFgtsakyLDALEKaglKPA-ETHDLSSLRTILSTGSPLKPESF 386
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 351 E----------RWESLTG-CRIVEGYGLTetSPVASTNPyGEL-AR-LGTvgipvadtAFKVIDDEGNELPlGERGEL-C 416
Cdd:cd05943 387 DyvydhikpdvLLASISGgTDIISCFVGG--NPLLPVYR-GEIqCRgLGM--------AVEAFDEEGKPVW-GEKGELvC 454
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 417 IKG-PQVMKGYWQqpeataqtlDADG--------------WlKTGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIED 481
Cdd:cd05943 455 TKPfPSMPVGFWN---------DPDGsryraayfakypgvW-AHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYR 524
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 482 VIMGHPKVANCAAIGVPDERSGEAVKLFVVAREGGLSVDEL----KAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRE 557
Cdd:cd05943 525 VVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDELrkriRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVA 604
|
....
gi 820875961 558 LRDI 561
Cdd:cd05943 605 VKKI 608
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
47-559 |
9.00e-10 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 60.90 E-value: 9.00e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 47 GKTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLI--VVNTNpLYTErEMRHQFKDAGAR 124
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQ-GYRSGDVVALFMENRLEFVALWLGLAKIGVEtaLINSN-LRLE-SLLHCITVSKAK 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 125 ALVYlnmfgkrvqevlpdtgieylieakmgdllpmakgwlinTVVDKVKKMVPAyHLPQAISfkqalregaalshkpvtL 204
Cdd:cd05939 78 ALIF--------------------------------------NLLDPLLTQSST-EPPSQDD-----------------V 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 205 SLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMLQVLACFSQHGPDgqklikegqeVMIAPLPLYHIyafTANCMCM--MV 282
Cdd:cd05939 102 NFRDKLFYIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGMRPED----------VVYDCLPLYHS---AGGIMGVgqAL 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 283 TGNHNVLITNPRDIPGFIKELGKWRFTALLGLNTLFVALMDHPgfRQLDFSALKVTNSGGTALvkaTAERWESLTG---- 358
Cdd:cd05939 169 LHGSTVVIRKKFSASNFWDDCVKYNCTIVQYIGEICRYLLAQP--PSEEEQKHNVRLAVGNGL---RPQIWEQFVRrfgi 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 359 CRIVEGYGLTE--TSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNELPLGERGeLCIK------GPQVMK------ 424
Cdd:cd05939 244 PQIGEFYGATEgnSSLVNIDNHVGACGFNSRILPSVYPIRLIKVDEDTGELIRDSDG-LCIPcqpgepGLLVGKiiqndp 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 425 -----GYWQQpEATAQTLDADGWLK------TGDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHPKVANCA 493
Cdd:cd05939 323 lrrfdGYVNE-GATNKKIARDVFKKgdsaflSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVV 401
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 820875961 494 AIGVP-DERSGEAVKLFVVAREGGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRRELR 559
Cdd:cd05939 402 VYGVEvPGVEGRAGMAAIVDPERKVDLDRFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQ 468
|
|
| PaaK |
cd05913 |
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ... |
333-490 |
1.55e-09 |
|
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.
Pssm-ID: 341239 [Multi-domain] Cd Length: 425 Bit Score: 60.33 E-value: 1.55e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 333 SALKVTNSGGTALVKATAERWESLTGCRIVEGYGLTETSPVASTNPYGElarlgTVGIPVADTAF--KVIDDEGNE-LPL 409
Cdd:cd05913 198 LSLKVGIFGAEPWTEEMRKRIERRLGIKAYDIYGLTEIIGPGVAFECEE-----KDGLHIWEDHFipEIIDPETGEpVPP 272
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 410 GERGELcikgpqvmkgywqqpeaTAQTLDADGWL----KTGDIAVIDPD----GFT-----RIVDRKKDMIIVSGFNVYP 476
Cdd:cd05913 273 GEVGEL-----------------VFTTLTKEAMPliryRTRDITRLLPGpcpcGRThrridRITGRSDDMLIIRGVNVFP 335
|
170
....*....|....
gi 820875961 477 NEIEDVIMGHPKVA 490
Cdd:cd05913 336 SQIEDVLLKIPGLG 349
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
333-558 |
3.06e-07 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 52.86 E-value: 3.06e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 333 SALKVTNSGGTALVKATA-ERW-ESLTGCRIVEGYGLTETSPVASTNPYGELARLGTVGIPVADTAFKVIDDEGNElplg 410
Cdd:cd17654 238 SSLRVLALGGEPFPSLVIlSSWrGKGNRTRIFNIYGITEVSCWALAYKVPEEDSPVQLGSPLLGTVIEVRDQNGSE---- 313
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 411 ERGELCIKGPQ---VMKGYWQQPEATaqtldadgWLKTGDIAVIDpDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIMGHP 487
Cdd:cd17654 314 GTGQVFLGGLNrvcILDDEVTVPKGT--------MRATGDFVTVK-DGELFFLGRKDSQIKRRGKRINLDLIQQVIESCL 384
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 820875961 488 KVANCAAIGVPDERsgeaVKLFVVARegGLSVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKILRREL 558
Cdd:cd17654 385 GVESCAVTLSDQQR----LIAFIVGE--SSSSRIHKELQLTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
385-560 |
4.33e-05 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 46.61 E-value: 4.33e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 385 LGTVGIPVADTAFKVIDDEGNELP-----LGErgelCIKGPQVM------------KGYWQ-QPEATAQTLDadgwlKTG 446
Cdd:PLN03052 524 FAAFSTPAMGCKLFILDDSGNPYPddapcTGE----LALFPLMFgasstllnadhyKVYFKgMPVFNGKILR-----RHG 594
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 447 DIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVI-MGHPKVANCAAIGVPDERSG-EAVKLFVV---AREGGLSVDE 521
Cdd:PLN03052 595 DIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPPGGGpEQLVIAAVlkdPPGSNPDLNE 674
|
170 180 190 200
....*....|....*....|....*....|....*....|....
gi 820875961 522 LK----AYCKANFTG-YKVpKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PLN03052 675 LKkifnSAIQKKLNPlFKV-SAVVIVPSFPRTASNKVMRRVLRQ 717
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
38-554 |
6.82e-05 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 45.56 E-value: 6.82e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 38 ADRPAFSNLG-----KTLTYGELDRLSAAFAAWLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTER 112
Cdd:PRK03584 98 DDRPAIIFRGedgprRELSWAELRRQVAALAAALRAL-GVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQ 176
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 113 EMRHQFKDAGARALV----YLnmFGKRVQEVLPDtgIEYLIEAkmgdlLPMakgwLINTV-VDKVKKMVPAYHLPQAISF 187
Cdd:PRK03584 177 GVLDRFGQIEPKVLIavdgYR--YGGKAFDRRAK--VAELRAA-----LPS----LEHVVvVPYLGPAAAAAALPGALLW 243
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 188 KQALREGAALSHKPVTLSLDD-IAVLqYTGGTTGLAKGAMLTHGNLVANMLQVLACfsqHGpDgqklIKEGQEVMIaplp 266
Cdd:PRK03584 244 EDFLAPAEAAELEFEPVPFDHpLWIL-YSSGTTGLPKCIVHGHGGILLEHLKELGL---HC-D----LGPGDRFFW---- 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 267 lyhiyaFTaNCMCMM----VTG-----------------NHNVLitnprdipgfikelgkWRFTALLGLnTLF------- 318
Cdd:PRK03584 311 ------YT-TCGWMMwnwlVSGllvgatlvlydgspfypDPNVL----------------WDLAAEEGV-TVFgtsakyl 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 319 VALMD---HPGfRQLDFSALKVTNSGGTAL-----------VKATAeRWESLT-GCRIVEGY-GLTETSPVAStnpyGEL 382
Cdd:PRK03584 367 DACEKaglVPG-ETHDLSALRTIGSTGSPLppegfdwvyehVKADV-WLASISgGTDICSCFvGGNPLLPVYR----GEI 440
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 383 -AR-LGtvgipvadTAFKVIDDEGNELpLGERGELCIKGPqvMK----GYWQqpeataqtlDADG--------------W 442
Cdd:PRK03584 441 qCRgLG--------MAVEAWDEDGRPV-VGEVGELVCTKP--FPsmplGFWN---------DPDGsryrdayfdtfpgvW 500
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 443 lKTGDIAVIDPDGftrivdrkkdmiivsGFNVY--------PN-------EIEDVIMGHPKVANCAAIGVPDERSGEAVK 507
Cdd:PRK03584 501 -RHGDWIEITEHG---------------GVVIYgrsdatlnRGgvrigtaEIYRQVEALPEVLDSLVIGQEWPDGDVRMP 564
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 820875961 508 LFVVAREG-----GLsVDELKAYCKANFTGYKVPKHIVLRESLPMTPVGKIL 554
Cdd:PRK03584 565 LFVVLAEGvtlddAL-RARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKV 615
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
446-560 |
2.72e-03 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 40.57 E-value: 2.72e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 820875961 446 GDIAVIDPDGFTRIVDRKKDMIIVSGFNVYPNEIEDVIM-GHPKVANCAAIGVPDERSGEAvKLFVVAREGGL------- 517
Cdd:PLN03051 362 GDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERACDrAVAGIAETAAVGVAPPDGGPE-LLVIFLVLGEEkkgfdqa 440
|
90 100 110 120
....*....|....*....|....*....|....*....|....*...
gi 820875961 518 SVDELKAYC----KANFTG-YKVpKHIVLRESLPMTPVGKILRRELRD 560
Cdd:PLN03051 441 RPEALQKKFqeaiQTNLNPlFKV-SRVKIVPELPRNASNKLLRRVLRD 487
|
|
| |
