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Conserved domains on  [gi|836688381|ref|WP_047835899|]
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MULTISPECIES: 2-hydroxychromene-2-carboxylate isomerase [Burkholderia]

Protein Classification

2-hydroxychromene-2-carboxylate isomerase( domain architecture ID 10122490)

2-hydroxychromene-2-carboxylate isomerase catalyzes the reversible glutathione-dependent isomerization of 2-hydroxychromene-2-carboxylate (HCCA) to trans-O-hydroxybenzylidenepyruvate (THBPA) in the naphthalene catabolic pathway

EC:  5.99.1.4
Gene Ontology:  GO:0018845|GO:1901170|GO:0043295
PubMed:  17508726

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DsbA_HCCA_Iso cd03022
DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a ...
 10-191 5.35e-49

DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a glutathione (GSH) dependent enzyme involved in the naphthalene catabolic pathway. It converts HCCA, a hemiketal formed spontaneously after ring cleavage of 1,2-dihydroxynapthalene by a dioxygenase, into cis-o-hydroxybenzylidenepyruvate (cHBPA). This is the fourth reaction in a six-step pathway that converts napthalene into salicylate. HCCA isomerase is unique to bacteria that degrade polycyclic aromatic compounds. It is closely related to the eukaryotic protein, GSH transferase kappa (GSTK).


:

Pssm-ID: 239320 [Multi-domain]  Cd Length: 192  Bit Score: 158.18  E-value: 5.35e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  10 PRWYYDFLSPFTYLLLEQQDKWP---SMPFEPVPVSLPVLQHHWGQRPMYDVPaKRVFTYRHALFR--ADQLGIPFRMPP 84
Cdd:cd03022    1 IDFYFDFSSPYSYLAHERLPALAarhGATVRYRPILLGGVFKATGNVPPANRP-PAKGRYRLRDLErwARRYGIPLRFPP 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  85 AHPFDSTQALRLAVAISAD----LATVREMFRFIWREGRDPTSEEGFAALCERVGVTPDD--PRLDSAQTREQLERNTQD 158
Cdd:cd03022   80 RFPPNTLRAMRAALAAQAEgdaaEAFARAVFRALWGEGLDIADPAVLAAVAAAAGLDADEllAAADDPAVKAALRANTEE 159

                        170       180       190
                 ....*....|....*....|....*....|...
gi 836688381 159 AIALGVFGVPTFWMNRQLFWGEDALPMVLYCAR 191
Cdd:cd03022  160 AIARGVFGVPTFVVDGEMFWGQDRLDMLEEALA 192
 
Name Accession Description Interval E-value
DsbA_HCCA_Iso cd03022
DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a ...
 10-191 5.35e-49

DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a glutathione (GSH) dependent enzyme involved in the naphthalene catabolic pathway. It converts HCCA, a hemiketal formed spontaneously after ring cleavage of 1,2-dihydroxynapthalene by a dioxygenase, into cis-o-hydroxybenzylidenepyruvate (cHBPA). This is the fourth reaction in a six-step pathway that converts napthalene into salicylate. HCCA isomerase is unique to bacteria that degrade polycyclic aromatic compounds. It is closely related to the eukaryotic protein, GSH transferase kappa (GSTK).


Pssm-ID: 239320 [Multi-domain]  Cd Length: 192  Bit Score: 158.18  E-value: 5.35e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  10 PRWYYDFLSPFTYLLLEQQDKWP---SMPFEPVPVSLPVLQHHWGQRPMYDVPaKRVFTYRHALFR--ADQLGIPFRMPP 84
Cdd:cd03022    1 IDFYFDFSSPYSYLAHERLPALAarhGATVRYRPILLGGVFKATGNVPPANRP-PAKGRYRLRDLErwARRYGIPLRFPP 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  85 AHPFDSTQALRLAVAISAD----LATVREMFRFIWREGRDPTSEEGFAALCERVGVTPDD--PRLDSAQTREQLERNTQD 158
Cdd:cd03022   80 RFPPNTLRAMRAALAAQAEgdaaEAFARAVFRALWGEGLDIADPAVLAAVAAAAGLDADEllAAADDPAVKAALRANTEE 159

                        170       180       190
                 ....*....|....*....|....*....|...
gi 836688381 159 AIALGVFGVPTFWMNRQLFWGEDALPMVLYCAR 191
Cdd:cd03022  160 AIARGVFGVPTFVVDGEMFWGQDRLDMLEEALA 192
NahD COG3917
2-hydroxychromene-2-carboxylate isomerase [Secondary metabolites biosynthesis, transport and ...
 11-193 3.27e-45

2-hydroxychromene-2-carboxylate isomerase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 443122 [Multi-domain]  Cd Length: 196  Bit Score: 148.78  E-value: 3.27e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  11 RWYYDFLSPFTYL-------LLEQQDKwpsmPFEPVPVSL-PVLQHHWGQRPMYDVPAKRVFTYRHALFRADQLGIPFRM 82
Cdd:COG3917    3 DFYFDFSSPYAYLaatrleaLAARHGA----EVRWRPVLLgAVFKATGGTPPAERIPAKGRYRLRDLARWARKLGLPFRF 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  83 PPAHPFDSTQALRLAVAISAD---LATVREMFRFIWREGRDPTSEEGFAALCERVGVTPD--DPRLDSAQTREQLERNTQ 157
Cdd:COG3917   79 PPHFPVNPLLAARAALAAQDAgaaAAFVRAVFRAVWAEGRDIADPAVLAAIAAAAGLDAAalLAAAQSPAVKARLRANTE 158

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 836688381 158 DAIALGVFGVPTFWMNRQLFWGEDALPMVLYCARTP 193
Cdd:COG3917  159 EAVARGVFGAPTFVVDGELFWGQDRLDFLEAALAGG 194
DSBA pfam01323
DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a ...
 13-188 6.51e-27

DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a thioredoxin-like structure pfam00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyze one step in prokaryotic polyaromatic hydrocarbon (PAH) catabolic pathways. This family also contains members with functions other than HCCA isomerization, such as Kappa family GSTs, whose similarity to HCCA isomerases was not previously recognized. The sequence Swiss:O07298 has been annotated as a dioxygenase but is almost certainly an HCCA isomerase enzyme. Similarly, the sequence Swiss:Q9ZI67 has been annotated as a dehydrogenase, but is most probably also an HCCA isomerase enzyme. In addition, the Rhizobium leguminosarum Swiss:Q52782 protein has been annotated as a putative glycerol-3-phosphate transfer protein, but is also most likely to be an HCCA isomerase enzyme.


Pssm-ID: 426200 [Multi-domain]  Cd Length: 191  Bit Score: 101.35  E-value: 6.51e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381   13 YYDFLSPFTYLLLEQQDK----WPSMPFEPVPVSLPVLQHHwGQRPMYDVPAKRVFTYRHALFRADQLGIPFRMPPAHPF 88
Cdd:pfam01323   5 FFDFLCPFCYLAKERLEKlaarYGDVKVVYRPFPLAGAKKI-GNVGPSNLPVKLKYMMADLERWAALYGIPLRFPANFLG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381   89 DSTQALRLAVAISA-DLAT--VREMFRFIWREGRDPTSEEGFAALCERVGVTPD--DPRLDSAQTREQLERNTQDAIALG 163
Cdd:pfam01323  84 NSTRANRLALAAGAeGLAEkvVRELFNALWGEGAAITDDSVLREVAEKAGLDAEefDEFLDSPAVKEAVRENTAAAISLG 163

                         170       180
                  ....*....|....*....|....*
gi 836688381  164 VFGVPTFWMNRQLFWGEDALPMVLY 188
Cdd:pfam01323 164 VFGVPTFVVGGKMVFGADRLDTLAD 188
 
Name Accession Description Interval E-value
DsbA_HCCA_Iso cd03022
DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a ...
 10-191 5.35e-49

DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a glutathione (GSH) dependent enzyme involved in the naphthalene catabolic pathway. It converts HCCA, a hemiketal formed spontaneously after ring cleavage of 1,2-dihydroxynapthalene by a dioxygenase, into cis-o-hydroxybenzylidenepyruvate (cHBPA). This is the fourth reaction in a six-step pathway that converts napthalene into salicylate. HCCA isomerase is unique to bacteria that degrade polycyclic aromatic compounds. It is closely related to the eukaryotic protein, GSH transferase kappa (GSTK).


Pssm-ID: 239320 [Multi-domain]  Cd Length: 192  Bit Score: 158.18  E-value: 5.35e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  10 PRWYYDFLSPFTYLLLEQQDKWP---SMPFEPVPVSLPVLQHHWGQRPMYDVPaKRVFTYRHALFR--ADQLGIPFRMPP 84
Cdd:cd03022    1 IDFYFDFSSPYSYLAHERLPALAarhGATVRYRPILLGGVFKATGNVPPANRP-PAKGRYRLRDLErwARRYGIPLRFPP 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  85 AHPFDSTQALRLAVAISAD----LATVREMFRFIWREGRDPTSEEGFAALCERVGVTPDD--PRLDSAQTREQLERNTQD 158
Cdd:cd03022   80 RFPPNTLRAMRAALAAQAEgdaaEAFARAVFRALWGEGLDIADPAVLAAVAAAAGLDADEllAAADDPAVKAALRANTEE 159

                        170       180       190
                 ....*....|....*....|....*....|...
gi 836688381 159 AIALGVFGVPTFWMNRQLFWGEDALPMVLYCAR 191
Cdd:cd03022  160 AIARGVFGVPTFVVDGEMFWGQDRLDMLEEALA 192
NahD COG3917
2-hydroxychromene-2-carboxylate isomerase [Secondary metabolites biosynthesis, transport and ...
 11-193 3.27e-45

2-hydroxychromene-2-carboxylate isomerase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 443122 [Multi-domain]  Cd Length: 196  Bit Score: 148.78  E-value: 3.27e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  11 RWYYDFLSPFTYL-------LLEQQDKwpsmPFEPVPVSL-PVLQHHWGQRPMYDVPAKRVFTYRHALFRADQLGIPFRM 82
Cdd:COG3917    3 DFYFDFSSPYAYLaatrleaLAARHGA----EVRWRPVLLgAVFKATGGTPPAERIPAKGRYRLRDLARWARKLGLPFRF 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  83 PPAHPFDSTQALRLAVAISAD---LATVREMFRFIWREGRDPTSEEGFAALCERVGVTPD--DPRLDSAQTREQLERNTQ 157
Cdd:COG3917   79 PPHFPVNPLLAARAALAAQDAgaaAAFVRAVFRAVWAEGRDIADPAVLAAIAAAAGLDAAalLAAAQSPAVKARLRANTE 158

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 836688381 158 DAIALGVFGVPTFWMNRQLFWGEDALPMVLYCARTP 193
Cdd:COG3917  159 EAVARGVFGAPTFVVDGELFWGQDRLDFLEAALAGG 194
DSBA pfam01323
DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a ...
 13-188 6.51e-27

DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a thioredoxin-like structure pfam00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyze one step in prokaryotic polyaromatic hydrocarbon (PAH) catabolic pathways. This family also contains members with functions other than HCCA isomerization, such as Kappa family GSTs, whose similarity to HCCA isomerases was not previously recognized. The sequence Swiss:O07298 has been annotated as a dioxygenase but is almost certainly an HCCA isomerase enzyme. Similarly, the sequence Swiss:Q9ZI67 has been annotated as a dehydrogenase, but is most probably also an HCCA isomerase enzyme. In addition, the Rhizobium leguminosarum Swiss:Q52782 protein has been annotated as a putative glycerol-3-phosphate transfer protein, but is also most likely to be an HCCA isomerase enzyme.


Pssm-ID: 426200 [Multi-domain]  Cd Length: 191  Bit Score: 101.35  E-value: 6.51e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381   13 YYDFLSPFTYLLLEQQDK----WPSMPFEPVPVSLPVLQHHwGQRPMYDVPAKRVFTYRHALFRADQLGIPFRMPPAHPF 88
Cdd:pfam01323   5 FFDFLCPFCYLAKERLEKlaarYGDVKVVYRPFPLAGAKKI-GNVGPSNLPVKLKYMMADLERWAALYGIPLRFPANFLG 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381   89 DSTQALRLAVAISA-DLAT--VREMFRFIWREGRDPTSEEGFAALCERVGVTPD--DPRLDSAQTREQLERNTQDAIALG 163
Cdd:pfam01323  84 NSTRANRLALAAGAeGLAEkvVRELFNALWGEGAAITDDSVLREVAEKAGLDAEefDEFLDSPAVKEAVRENTAAAISLG 163

                         170       180
                  ....*....|....*....|....*
gi 836688381  164 VFGVPTFWMNRQLFWGEDALPMVLY 188
Cdd:pfam01323 164 VFGVPTFVVGGKMVFGADRLDTLAD 188
DsbA_GSTK cd03021
DsbA family, Glutathione (GSH) S-transferase Kappa (GSTK) subfamily; GSTK is a member of the ...
 13-186 4.71e-15

DsbA family, Glutathione (GSH) S-transferase Kappa (GSTK) subfamily; GSTK is a member of the GST family of enzymes which catalyzes the transfer of the thiol of GSH to electrophilic substrates. It is specifically located in the mitochondria and peroxisomes, unlike other members of the canonical GST family, which are mainly cytosolic. The biological substrates of GSTK are not yet known. It is presumed to have a protective role during respiration when large amounts of reactive oxygen species are generated. GSTK has the same general fold as DsbA, consisting of a thioredoxin domain interrupted by an alpha-helical domain and its biological unit is a homodimer. GSTK is closely related to the bacterial enzyme, 2-hydroxychromene-2-carboxylate (HCCA) isomerase. It shows little sequence similarity to the other members of the GST family.


Pssm-ID: 239319 [Multi-domain]  Cd Length: 209  Bit Score: 70.84  E-value: 4.71e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  13 YYDFLSPFTYL---LLEQQDKWPSMPFEPVPVSLPVLQHHWGQRPMYDVPAKRVFTYRHALFRADQLGIPFRMPPAHPFD 89
Cdd:cd03021    6 YYDVVSPYSYLafeVLCRYQTAWNVDITYVPVFLGGIMKDSGNKPPIMLPNKAKYMAKDRKRSAEFFGVPIRQPKDFFFM 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  90 ---STQALRLAVAISA-----DLATVREMFRFIWREG----RDPTSEEGFAALCERVGVTPDDPR-----LDSAQTREQL 152
Cdd:cd03021   86 kkgTLTAQRFLTAISEqhpesTLTALEALFREFWVRPwsltEPITESQSISVAADKLGGSAEQAEkllkaASTPEVKNRL 165

                        170       180       190
                 ....*....|....*....|....*....|....*....
gi 836688381 153 ERNTQDAIALGVFGVPTFWMNR-----QLFWGEDALPMV 186
Cdd:cd03021  166 KENTDEALKYGAFGLPWIVVTNdkgktEMFFGSDRFEQV 204
FrnE COG2761
Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) ...
 13-176 2.69e-12

Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442047 [Multi-domain]  Cd Length: 205  Bit Score: 62.98  E-value: 2.69e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  13 YYDFLSPFTYLL---LEQ-QDKWPS------MPFEPVPvSLPvlqhHWGQRPMYDVPAKRVFTYRHALFR-----ADQLG 77
Cdd:COG2761    7 FSDVVCPWCYIGkrrLEKaLAEFGDdveirwRPFELNP-DMP----PEGEDRREYLLAKGSPEQAEQMRAhveeaAAEEG 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  78 IPFRMP---PAHPFDSTQALRLAVAISADLATVREMFRFIWREGRDPTSEEGFAALCERVGVTPDDPR--LDSAQTREQL 152
Cdd:COG2761   82 LPFDFDrikPPNTFDAHRLLKAAELQGKQDALLEALFEAYFTEGRDIGDREVLLDLAAEVGLDAEEFRadLESDEAAAAV 161

                        170       180
                 ....*....|....*....|....
gi 836688381 153 ERNTQDAIALGVFGVPTFWMNRQL 176
Cdd:COG2761  162 RADEAEARELGVTGVPTFVFDGKY 185
DsbA_FrnE cd03024
DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is ...
 72-175 2.29e-08

DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


Pssm-ID: 239322 [Multi-domain]  Cd Length: 201  Bit Score: 52.20  E-value: 2.29e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  72 RADQLGIPFRMPPAHP---FDSTQALRLAVAISADLATVREMFRFIWREGRDPTSEEGFAALCERVGVTPDDPR--LDSA 146
Cdd:cd03024   76 AAAAEGLEFDFDRVRPpntFDAHRLIHLAKEQGKQDALVEALFRAYFTEGKDIGDRDVLVDLAEEAGLDAAEARavLASD 155

                         90       100
                 ....*....|....*....|....*....
gi 836688381 147 QTREQLERNTQDAIALGVFGVPTFWMNRQ 175
Cdd:cd03024  156 EYADEVRADEARARQLGISGVPFFVFNGK 184
DsbG COG1651
Protein thiol-disulfide isomerase DsbC [Posttranslational modification, protein turnover, ...
 87-179 6.59e-08

Protein thiol-disulfide isomerase DsbC [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441257 [Multi-domain]  Cd Length: 152  Bit Score: 50.00  E-value: 6.59e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  87 PFDSTQALRLAVAI--SADLATVREMFRFIWREGRDPTsEEGFAALCERVGVTPDDPR--LDSAQTREQLERNTQDAIAL 162
Cdd:COG1651   42 PLLHPDSLRAARAAlcAADQGKFWAFHDALFANQPALT-DDDLREIAKEAGLDAAKFDacLNSGAVAAKVEADTALAQAL 120

                         90
                 ....*....|....*..
gi 836688381 163 GVFGVPTFWMNRQLFWG 179
Cdd:COG1651  121 GVTGTPTFVVNGKLVSG 137
COG3531 COG3531
Predicted protein-disulfide isomerase, contains CxxC motif [Posttranslational modification, ...
 86-170 3.79e-05

Predicted protein-disulfide isomerase, contains CxxC motif [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442753 [Multi-domain]  Cd Length: 206  Bit Score: 42.93  E-value: 3.79e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  86 HPFDSTQALRlAVAISADLATVRE--MFRFI----WREGRDPTSEEGFAALCERVGVTPDD--PRLDSAQTREQLERNTQ 157
Cdd:COG3531   87 FVLDSEPACR-AVLAARELAPERElaMLHAIqrafYVEGRDISDPEVLAELAAELGLDAEAfaAALASEETRQHIQQEFA 165

                         90
                 ....*....|...
gi 836688381 158 DAIALGVFGVPTF 170
Cdd:COG3531  166 LARQLGVQGFPTL 178
DsbA_FrnE_like cd03025
DsbA family, FrnE-like subfamily; composed of uncharacterized proteins containing a CXXC motif ...
 42-170 1.31e-04

DsbA family, FrnE-like subfamily; composed of uncharacterized proteins containing a CXXC motif with similarity to DsbA and FrnE. FrnE is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


Pssm-ID: 239323 [Multi-domain]  Cd Length: 193  Bit Score: 41.15  E-value: 1.31e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 836688381  42 SLPVLQHHWGQRP--MYDVPAKRVFTYRHALFRADQL-GIPFRMPPAHPFDS-------TQALRLAVAISADLAtvREMF 111
Cdd:cd03025   31 GIEVELHLGGLLPgnNARQITKQWRIYVHWHKARIALtGQPFGEDYLELLLFdldsapaSRAIKAARLQGPERL--LEML 108

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 836688381 112 RFIWR----EGRDPTSEEGFAALCERVGVTPDD--PRLDSAQTREQLERNTQDAIALGVFGVPTF 170
Cdd:cd03025  109 KAIQRahyvEGRDLADTEVLRELAIELGLDVEEflEDFQSDEAKQAIQEDQKLARELGINGFPTL 173
DsbA_family cd02972
DsbA family; consists of DsbA and DsbA-like proteins, including DsbC, DsbG, glutathione (GSH) ...
 154-181 2.85e-04

DsbA family; consists of DsbA and DsbA-like proteins, including DsbC, DsbG, glutathione (GSH) S-transferase kappa (GSTK), 2-hydroxychromene-2-carboxylate (HCCA) isomerase, an oxidoreductase (FrnE) presumed to be involved in frenolicin biosynthesis, a 27-kDa outer membrane protein, and similar proteins. Members of this family contain a redox active CXXC motif (except GSTK and HCCA isomerase) imbedded in a TRX fold, and an alpha helical insert of about 75 residues (shorter in DsbC and DsbG) relative to TRX. DsbA is involved in the oxidative protein folding pathway in prokaryotes, catalyzing disulfide bond formation of proteins secreted into the bacterial periplasm. DsbC and DsbG function as protein disulfide isomerases and chaperones to correct non-native disulfide bonds formed by DsbA and prevent aggregation of incorrectly folded proteins.


Pssm-ID: 239270 [Multi-domain]  Cd Length: 98  Bit Score: 38.93  E-value: 2.85e-04
                         10        20
                 ....*....|....*....|....*...
gi 836688381 154 RNTQDAIALGVFGVPTFWMNRQLFWGED 181
Cdd:cd02972   71 ADTALARALGVTGTPTFVVNGEKYSGAG 98
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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