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Conserved domains on  [gi|880805691|ref|WP_048657871|]
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MULTISPECIES: type IV pilin protein [Vibrio]

Protein Classification

type IV pilin protein( domain architecture ID 11471939)

type IV pilin protein similar to Pseudomonas aeruginosa type IV pilus (T4P) non-core minor pilin PilE, an ssential component of the T4P that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, twitching, adhesion, and retraction of T4P fibers

Gene Ontology:  GO:0043683|GO:0016020
PubMed:  7854130|20338182

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
14-142 1.42e-27

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


:

Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 98.99  E-value: 1.42e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  14 MRGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAHRVTAMADMTKIQLEIETLY--TGNYASAAvniisgGTCLFCDTDS 91
Cdd:COG4968    9 QRGFTLIELMIVVAIIGILAAIAIPSYQDYVERARRAEAKAALLELAQAQERYYadNGSYPSAL------ADLGLPASTS 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 880805691  92 SRYTLAISASSSTYSIQAEPHApqtsddclDSTtdiLELHHSGI--SEPEACW 142
Cdd:COG4968   83 GYYTISIAAAATTYTLTATPQA--------DGT---LTLDQTGVktATGADCW 124
 
Name Accession Description Interval E-value
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
14-142 1.42e-27

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 98.99  E-value: 1.42e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  14 MRGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAHRVTAMADMTKIQLEIETLY--TGNYASAAvniisgGTCLFCDTDS 91
Cdd:COG4968    9 QRGFTLIELMIVVAIIGILAAIAIPSYQDYVERARRAEAKAALLELAQAQERYYadNGSYPSAL------ADLGLPASTS 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 880805691  92 SRYTLAISASSSTYSIQAEPHApqtsddclDSTtdiLELHHSGI--SEPEACW 142
Cdd:COG4968   83 GYYTISIAAAATTYTLTATPQA--------DGT---LTLDQTGVktATGADCW 124
PRK10574 PRK10574
putative major pilin subunit; Provisional
 15-99 4.74e-10

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 54.27  E-value: 4.74e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  15 RGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAhrvtAMADMT------KIQLEIETLYTGNYAS--AAVNIISGGTclf 86
Cdd:PRK10574   5 RGFTLIELMVVIAIIAILSAIGIPAYQNYLQKA----ALTDMLqtfvpyKTAVELCALEHGGLDTcdAGSNGIPSPT--- 77

                         90
                 ....*....|...
gi 880805691  87 cdtdSSRYTLAIS 99
Cdd:PRK10574  78 ----TSRYVSAMS 86
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 15-37 9.29e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 37.35  E-value: 9.29e-05
                          10        20
                  ....*....|....*....|...
gi 880805691   15 RGMTLIELLLAVVIVGILGAIAY 37
Cdd:pfam07963   5 RGFTLIELLVALAILAILLAAAL 27
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 15-37 1.39e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 36.90  E-value: 1.39e-04
                          10        20
                  ....*....|....*....|...
gi 880805691   15 RGMTLIELLLAVVIVGILGAIAY 37
Cdd:TIGR02532   2 RGFTLIELLVVLAILGILALIAL 24
 
Name Accession Description Interval E-value
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
14-142 1.42e-27

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 98.99  E-value: 1.42e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  14 MRGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAHRVTAMADMTKIQLEIETLY--TGNYASAAvniisgGTCLFCDTDS 91
Cdd:COG4968    9 QRGFTLIELMIVVAIIGILAAIAIPSYQDYVERARRAEAKAALLELAQAQERYYadNGSYPSAL------ADLGLPASTS 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 880805691  92 SRYTLAISASSSTYSIQAEPHApqtsddclDSTtdiLELHHSGI--SEPEACW 142
Cdd:COG4968   83 GYYTISIAAAATTYTLTATPQA--------DGT---LTLDQTGVktATGADCW 124
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
15-107 2.82e-13

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 62.42  E-value: 2.82e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  15 RGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAhRVTAM-ADMTKIQLEIETLYTGNYASAAVNIISGGtclFCDTDSSR 93
Cdd:COG4969    6 KGFTLIELMIVVAIIGILAAIAIPAYQDYVARA-RVSEAlALASPLKTAVEECALENGSLPNCNAGDNG---LPATIATK 81

                         90
                 ....*....|....
gi 880805691  94 YTLAISASSSTYSI 107
Cdd:COG4969   82 YVSSVSVDNGVITV 95
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-70 7.90e-13

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 60.31  E-value: 7.90e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691   1 MIRRNICNSNnismRGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAHRVTAMADMTKIQLEIETLYTGN 70
Cdd:COG2165    1 MKLRRRRRRQ----RGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLRQIQQALERYRLDN 66
PRK10574 PRK10574
putative major pilin subunit; Provisional
 15-99 4.74e-10

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 54.27  E-value: 4.74e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 880805691  15 RGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAhrvtAMADMT------KIQLEIETLYTGNYAS--AAVNIISGGTclf 86
Cdd:PRK10574   5 RGFTLIELMVVIAIIAILSAIGIPAYQNYLQKA----ALTDMLqtfvpyKTAVELCALEHGGLDTcdAGSNGIPSPT--- 77

                         90
                 ....*....|...
gi 880805691  87 cdtdSSRYTLAIS 99
Cdd:PRK10574  78 ----TSRYVSAMS 86
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
15-55 1.17e-08

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 48.69  E-value: 1.17e-08
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 880805691  15 RGMTLIELLLAVVIVGILGAIAYPSYTNHVTKaHRVTAMAD 55
Cdd:COG4970    9 RGFTLIELLVVLAILAILAAIAVPSFSSLIAR-QRLRAAAN 48
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 15-37 8.77e-05

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 39.61  E-value: 8.77e-05
                         10        20
                 ....*....|....*....|...
gi 880805691  15 RGMTLIELLLAVVIVGILGAIAY 37
Cdd:COG4795    9 RGFTLLELLVALAIFALLLLAAY 31
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 15-37 9.29e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 37.35  E-value: 9.29e-05
                          10        20
                  ....*....|....*....|...
gi 880805691   15 RGMTLIELLLAVVIVGILGAIAY 37
Cdd:pfam07963   5 RGFTLIELLVALAILAILLAAAL 27
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 15-37 1.39e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 36.90  E-value: 1.39e-04
                          10        20
                  ....*....|....*....|...
gi 880805691   15 RGMTLIELLLAVVIVGILGAIAY 37
Cdd:TIGR02532   2 RGFTLIELLVVLAILGILALIAL 24
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
13-56 4.23e-04

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 38.24  E-value: 4.23e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 880805691  13 SMRGMTLIELLLAVVIVGILGAIAYPSYTNHVTKAHRVTAMADM 56
Cdd:COG4966    3 RQRGFTLVELMVALAIGLIVLAAVLQLFLSSRRSYRTQEALARL 46
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-37 5.88e-04

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 36.50  E-value: 5.88e-04
                         10        20        30
                 ....*....|....*....|....*....|....*....
gi 880805691   1 MIRRNICNSnnisMRGMTLIELLLAVVI--VGILGAIAY 37
Cdd:COG4967    1 MSRRRRRRR----QRGFTLIEVLVALVIlsIGLLGLAGL 35
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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