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Conserved domains on  [gi|948069797|ref|WP_056728631|]
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MULTISPECIES: polynucleotide adenylyltransferase PcnB [Pseudomonas]

Protein Classification

polynucleotide adenylyltransferase PcnB( domain architecture ID 11493326)

polynucleotide adenylyltransferase PcnB, or poly(A) polymerase, creates the 3' poly(A) tail found in some mRNAs

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-446 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


:

Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 593.70  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797   25 VLNSGQHSLQKAQFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  105 IHFGREIIEVATFRANHpqnedeedsnQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIR 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGH----------KSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLK 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  185 NHLIRLIGDPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVD 264
Cdd:TIGR01942 151 NRRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCG 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  265 LQLFDPLFPASAEALEYNPTYTHTLISEALIN-TDLRIKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEAA 343
Cdd:TIGR01942 231 YQLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAI 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  344 HELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQDANE 423
Cdd:TIGR01942 311 RDFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASE 390

                         410       420
                  ....*....|....*....|...
gi 948069797  424 SERRDMIRdlsGKDEGTGaPRKR 446
Cdd:TIGR01942 391 EERTEMTR---SAARHEG-NRGR 409
 
Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-446 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 593.70  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797   25 VLNSGQHSLQKAQFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  105 IHFGREIIEVATFRANHpqnedeedsnQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIR 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGH----------KSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLK 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  185 NHLIRLIGDPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVD 264
Cdd:TIGR01942 151 NRRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCG 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  265 LQLFDPLFPASAEALEYNPTYTHTLISEALIN-TDLRIKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEAA 343
Cdd:TIGR01942 231 YQLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAI 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  344 HELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQDANE 423
Cdd:TIGR01942 311 RDFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASE 390

                         410       420
                  ....*....|....*....|...
gi 948069797  424 SERRDMIRdlsGKDEGTGaPRKR 446
Cdd:TIGR01942 391 EERTEMTR---SAARHEG-NRGR 409
pcnB PRK11623
poly(A) polymerase I; Provisional
 30-463 1.62e-157

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 454.59  E-value: 1.62e-157
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  30 QHSLQKAQFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGR 109
Cdd:PRK11623  43 QHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGP 122

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 110 EIIEVATFRANHPQNEdeEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNHLIR 189
Cdd:PRK11623 123 EIIEVATFRGHHEGNE--SDRNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKEGVIR 200

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 190 LIGDPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFD 269
Cdd:PRK11623 201 LIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQ 280

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 270 PLFPASAEAL-EYNPTYTHTLISEALINTDLRIKQNKPVTPAFLFAALLWPALPARVLRL-QERGMPPIPAMQEAAHELI 347
Cdd:PRK11623 281 PLFPTITRYFtENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIaQESGLAYYDAFALAMNDVL 360

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 348 AEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTD--GLGEWWTDYQDANESE 425
Cdd:PRK11623 361 DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVENNAElqRLAQWWGEFQVSAPPQ 440

                        410       420       430
                 ....*....|....*....|....*....|....*...
gi 948069797 426 RRDMIRDLsGKDegtGAPRKRRRsggSKRKRAAGASST 463
Cdd:PRK11623 441 QKGMLNEL-DDD---PAPRRRTR---RPRKRAPRREGT 471
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 37-437 2.83e-119

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 354.12  E-value: 2.83e-119
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  37 QFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFR---NARIIGRRFKLVHIHFGREIIE 113
Cdd:COG0617    1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 114 VATFRANHpqneDEEDsnqssrnesGRiLRDNVYG-TLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNHLIRLIG 192
Cdd:COG0617   81 VATARTER----YYGD---------GR-RPFVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVG 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 193 DPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFDPLf 272
Cdd:COG0617  147 DPEERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL- 225

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 273 pasaealeynptythtlisealintdlrikqnkpvtpAFLFAALLWPALPARVlrlQERGMPPIPAMQEAAHELIAEQCQ 352
Cdd:COG0617  226 -------------------------------------ALRLAALLHDLGKPAT---REDGLPTFHGHEEAGAELAEALLK 265

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 353 RIAIPKRFTMPIREIWDMQERLP-----RRSGKRADLlldNPRFRAGYDFLLLRESAGEQTDGLGEWWtdyqDANESERR 427
Cdd:COG0617  266 RLRLPNRERKLVRELVELHLRFHglgelRDSAVRRLL---ERGPEALEDLLLLRENGLEYPELQERLA----ELLEAAWR 338

                        410
                 ....*....|
gi 948069797 428 DMIRDLSGKD 437
Cdd:COG0617  339 RFQPPVDGED 348
PolyA_pol_arg_C pfam12626
Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is ...
 331-448 1.46e-52

Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is arginine-rich and is necessary for full functioning of the enzyme.


Pssm-ID: 463647 [Multi-domain]  Cd Length: 116  Bit Score: 172.77  E-value: 1.46e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  331 RGMPPIPAMQEAAHELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDG 410
Cdd:pfam12626   1 QGLPPYDALQLAADEVLDEQCQRVAIPRRFTAPMREIWLLQPRLEKRQGKRPFRLLEHPRFRAAYDFLLLRAESGEVLQE 80

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 948069797  411 LGEWWTDYQDANESERRDMIRDLSgkdEGTGAPRKRRR 448
Cdd:pfam12626  81 LAEWWTRFQEADEAEREAMIAELG---ADAAKKKRRRR 115
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 41-185 3.03e-40

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 141.19  E-value: 3.03e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  41 YAVNIVERLQNA-GYQAYLVGGCVRDMLLGITPKDFDVATSA-TPEQVR--AEFRNARIIGR--RFKLVHIHFGREIIEV 114
Cdd:cd05398    3 ELLKLLRELKKAlGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEalFKKIGGRVVGLgeEFGTATVVINGLTIDV 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 948069797 115 ATFRanhpqnedeedsnqSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 185
Cdd:cd05398   83 ATLR--------------TETYTDPGRRPPVVGFTIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
 
Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-446 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 593.70  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797   25 VLNSGQHSLQKAQFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  105 IHFGREIIEVATFRANHpqnedeedsnQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIR 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGH----------KSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLK 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  185 NHLIRLIGDPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVD 264
Cdd:TIGR01942 151 NRRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCG 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  265 LQLFDPLFPASAEALEYNPTYTHTLISEALIN-TDLRIKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEAA 343
Cdd:TIGR01942 231 YQLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAI 310

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  344 HELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQDANE 423
Cdd:TIGR01942 311 RDFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASE 390

                         410       420
                  ....*....|....*....|...
gi 948069797  424 SERRDMIRdlsGKDEGTGaPRKR 446
Cdd:TIGR01942 391 EERTEMTR---SAARHEG-NRGR 409
pcnB PRK11623
poly(A) polymerase I; Provisional
 30-463 1.62e-157

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 454.59  E-value: 1.62e-157
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  30 QHSLQKAQFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGR 109
Cdd:PRK11623  43 QHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGP 122

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 110 EIIEVATFRANHPQNEdeEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNHLIR 189
Cdd:PRK11623 123 EIIEVATFRGHHEGNE--SDRNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKEGVIR 200

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 190 LIGDPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFD 269
Cdd:PRK11623 201 LIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQ 280

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 270 PLFPASAEAL-EYNPTYTHTLISEALINTDLRIKQNKPVTPAFLFAALLWPALPARVLRL-QERGMPPIPAMQEAAHELI 347
Cdd:PRK11623 281 PLFPTITRYFtENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIaQESGLAYYDAFALAMNDVL 360

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 348 AEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTD--GLGEWWTDYQDANESE 425
Cdd:PRK11623 361 DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVENNAElqRLAQWWGEFQVSAPPQ 440

                        410       420       430
                 ....*....|....*....|....*....|....*...
gi 948069797 426 RRDMIRDLsGKDegtGAPRKRRRsggSKRKRAAGASST 463
Cdd:PRK11623 441 QKGMLNEL-DDD---PAPRRRTR---RPRKRAPRREGT 471
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 37-437 2.83e-119

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 354.12  E-value: 2.83e-119
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  37 QFSRYAVNIVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFR---NARIIGRRFKLVHIHFGREIIE 113
Cdd:COG0617    1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 114 VATFRANHpqneDEEDsnqssrnesGRiLRDNVYG-TLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNHLIRLIG 192
Cdd:COG0617   81 VATARTER----YYGD---------GR-RPFVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVG 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 193 DPTQRYQEDPVRMLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFDPLf 272
Cdd:COG0617  147 DPEERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL- 225

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 273 pasaealeynptythtlisealintdlrikqnkpvtpAFLFAALLWPALPARVlrlQERGMPPIPAMQEAAHELIAEQCQ 352
Cdd:COG0617  226 -------------------------------------ALRLAALLHDLGKPAT---REDGLPTFHGHEEAGAELAEALLK 265

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 353 RIAIPKRFTMPIREIWDMQERLP-----RRSGKRADLlldNPRFRAGYDFLLLRESAGEQTDGLGEWWtdyqDANESERR 427
Cdd:COG0617  266 RLRLPNRERKLVRELVELHLRFHglgelRDSAVRRLL---ERGPEALEDLLLLRENGLEYPELQERLA----ELLEAAWR 338

                        410
                 ....*....|
gi 948069797 428 DMIRDLSGKD 437
Cdd:COG0617  339 RFQPPVDGED 348
PRK13299 PRK13299
tRNA CCA-pyrophosphorylase; Provisional
 45-279 4.13e-57

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237339 [Multi-domain]  Cd Length: 394  Bit Score: 193.90  E-value: 4.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  45 IVERLQNAGYQAYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRAnhpqn 124
Cdd:PRK13299  12 ILEKIKEAGFEAYFVGGSVRDYLLGRPIHDVDIATSAYPEEVKAIFPRTVDVGIEHGTVLVLENGEEYEVTTFRT----- 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 125 edEEDSNQSSRNESGRILRDnvygtLEEDAQRRDFTINALYYDpVSERILDYANGVHDIRNHLIRLIGDPTQRYQEDPVR 204
Cdd:PRK13299  87 --ESEYVDYRRPSEVTFVRS-----LEEDLKRRDFTINAIAMD-ENGEIIDLFDGLEDLKNRLIRAVGNAEERFQEDALR 158

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 948069797 205 MLRAVRFAAKLNFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFD--PLFPASAEAL 279
Cdd:PRK13299 159 MMRAVRFASQLGFDLETETFEAMKTQAPLLEKISVERIFVEFEKLLLGPFWRKGLKLLIETGLYNylPGLKGKEENL 235
PolyA_pol_arg_C pfam12626
Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is ...
 331-448 1.46e-52

Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is arginine-rich and is necessary for full functioning of the enzyme.


Pssm-ID: 463647 [Multi-domain]  Cd Length: 116  Bit Score: 172.77  E-value: 1.46e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  331 RGMPPIPAMQEAAHELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDG 410
Cdd:pfam12626   1 QGLPPYDALQLAADEVLDEQCQRVAIPRRFTAPMREIWLLQPRLEKRQGKRPFRLLEHPRFRAAYDFLLLRAESGEVLQE 80

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 948069797  411 LGEWWTDYQDANESERRDMIRDLSgkdEGTGAPRKRRR 448
Cdd:pfam12626  81 LAEWWTRFQEADEAEREAMIAELG---ADAAKKKRRRR 115
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
 56-189 2.83e-49

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 164.38  E-value: 2.83e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797   56 AYLVGGCVRDMLLGITPKDFDVATSATPEQVRAEFRNARII----GRRFKLVHIHFGREIIEVATFRANHpqneDEEDsn 131
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRRRIVhllsGIEFGTIHVIFGNQILEVATFRIEF----DESD-- 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 948069797  132 qssrnesGRILRDNVY-GTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNHLIR 189
Cdd:pfam01743  75 -------FRNPRSEEYtGTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 41-185 3.03e-40

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 141.19  E-value: 3.03e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  41 YAVNIVERLQNA-GYQAYLVGGCVRDMLLGITPKDFDVATSA-TPEQVR--AEFRNARIIGR--RFKLVHIHFGREIIEV 114
Cdd:cd05398    3 ELLKLLRELKKAlGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEalFKKIGGRVVGLgeEFGTATVVINGLTIDV 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 948069797 115 ATFRanhpqnedeedsnqSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 185
Cdd:cd05398   83 ATLR--------------TETYTDPGRRPPVVGFTIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
PRK13296 PRK13296
CCA tRNA nucleotidyltransferase;
57-305 3.92e-21

CCA tRNA nucleotidyltransferase;


Pssm-ID: 106256 [Multi-domain]  Cd Length: 360  Bit Score: 94.67  E-value: 3.92e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  57 YLVGGCVRDMLLGITPKDFD-VATSATPEQvraefrnarIIGRRFKLVHIHFGREIievatfranHPQNEDE----EDSN 131
Cdd:PRK13296   4 YLVGGAVRDMLLGITPKDKDwVVVGATEDE---------MLANGFIKIAANFPVFI---------HPQTKQEyalaRSEK 65

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 132 QSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNHLIRligDPTQRYQEDPVRMLRAVRF 211
Cdd:PRK13296  66 KTASGYHGFEVNFSKYITLEDDLKRRDLTINSIAIDQ-NNKVIDPFNGQADLQNRILR---HTSIAFIEDPLRVVRLARF 141

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 212 AAKL---NFGIEKHSALPIRGLAPM--LREIPSARLFEEVLKLFlsGHAADTFEMLVDLQLFDPLFPASAEALEY----- 281
Cdd:PRK13296 142 KAQLsnfNFSIAQEMLALIKELVKTgeLNHLTRERLHIEFVKAL--NNPKIFFTTLKELEALKIIFPNISCILPLipnks 219

                        250       260
                 ....*....|....*....|....*..
gi 948069797 282 ---NPTYTHTLISEALINTDLRIKQNK 305
Cdd:PRK13296 220 ffeNPIYKGSNINEKITLCLLKIPQQQ 246
PolyA_pol_RNAbd pfam12627
Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA ...
 216-279 5.46e-18

Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA and SrmB binding motifs on polymerase A.


Pssm-ID: 463648 [Multi-domain]  Cd Length: 64  Bit Score: 77.91  E-value: 5.46e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 948069797  216 NFGIEKHSALPIRGLAPMLREIPSARLFEEVLKLFLSGHAADTFEMLVDLQLFDPLFPASAEAL 279
Cdd:pfam12627   1 GFTIEPETREAIRKLAPLLKKISPERIFEELLKLLLSGHPERGLELLRETGLLEYLFPELAAAL 64
PRK13298 PRK13298
tRNA CCA-pyrophosphorylase; Provisional
 57-221 6.41e-18

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237338 [Multi-domain]  Cd Length: 417  Bit Score: 85.55  E-value: 6.41e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  57 YLVGGCVRDMLLGITPKDFD-VATSATPEQVRAefRNARIIGRRFKlVHIHfgreiievatfranhPQNEDE----EDSN 131
Cdd:PRK13298   4 YLVGGAVRDSLLNLPVKDKDwVVVGGTPKILLS--INFQQVGKDFP-VFLH---------------PETHEEyalaRTER 65

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 132 QSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNHLIRLIgdpTQRYQEDPVRMLRAVRF 211
Cdd:PRK13298  66 KSGVGYTGFITDTSSDVTLEEDLIRRDLTINAIAQDE-NGNYIDPFQGKKDIQLRLLRHV---SESFIEDPLRVLRVARF 141

                        170
                 ....*....|...
gi 948069797 212 AAK---LNFGIEK 221
Cdd:PRK13298 142 AALlvhLGFKIAK 154
PRK13297 PRK13297
tRNA CCA-pyrophosphorylase; Provisional
 52-215 1.08e-17

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 139469 [Multi-domain]  Cd Length: 364  Bit Score: 84.28  E-value: 1.08e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  52 AGYQAYLVGGCVRDMLLGITPKDFD-VATSATPEQVraefrnARiigRRFKLVHIHFGREIievatfranHPQNEDE--- 127
Cdd:PRK13297  10 AGLQVYIVGGAVRDALLGLPAGDRDwVVVGATPEDM------AR---RGFIPVGGDFPVFL---------HPRTKEEyal 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 128 -EDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSErILDYANGVHDIRNHLIRLIGDPtqrYQEDPVRML 206
Cdd:PRK13297  72 aRTERKSGRGYKGFTFYTGADVTLEQDLQRRDLTVNAIARTPQGE-LVDPLDGVADVRARVLRHVGEA---FAEDPVRIL 147


                 ....*....
gi 948069797 207 RAVRFAAKL 215
Cdd:PRK13297 148 RLGRFAARF 156
cca PRK10885
multifunctional CCA addition/repair protein;
55-219 1.11e-17

multifunctional CCA addition/repair protein;


Pssm-ID: 182810 [Multi-domain]  Cd Length: 409  Bit Score: 84.91  E-value: 1.11e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797  55 QAYLVGGCVRDMLLGITPKDFD-VATSATPEQVRAefRNARIIGRRFKLvhihfgreiievatFRanHPQNEDEEDSNQS 133
Cdd:PRK10885   2 KIYLVGGAVRDALLGLPVKDRDwVVVGATPEEMLA--QGYQQVGKDFPV--------------FL--HPKTHEEYALART 63

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 948069797 134 SRnESGRilrdnVYG----------TLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNHLIRLIGDPtqrYQEDPV 203
Cdd:PRK10885  64 ER-KSGR-----GYTgftcyaapdvTLEEDLIRRDLTINAMAQDD-DGELIDPYGGQRDLEARLLRHVSPA---FAEDPL 133

                        170
                 ....*....|....*....
gi 948069797 204 RMLRAVRFAAK---LNFGI 219
Cdd:PRK10885 134 RVLRVARFAARfahLGFRI 152
PHA01806 PHA01806
hypothetical protein
 56-89 1.78e-04

hypothetical protein


Pssm-ID: 222838  Cd Length: 200  Bit Score: 42.56  E-value: 1.78e-04
                         10        20        30
                 ....*....|....*....|....*....|....
gi 948069797  56 AYLVGGCVRDMLLGITPKDFDVATsATPEQVRAE 89
Cdd:PHA01806  38 VALAGGAARDLMHGAEPKDIDIAL-YGMDDRQAE 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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