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Conserved domains on  [gi|970310189|ref|WP_058679550|]
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polymorphic toxin type 44 domain-containing protein [Enterobacter cloacae]

Protein Classification

PAAR domain-containing protein( domain architecture ID 10201018)

PAAR (proline-alanine-alanine-arginine) domain-containing protein forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ntox44 pfam15607
Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The ...
 212-319 1.52e-32

Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The toxin possesses an all-alpha-helical fold with conserved DxK, GNxxxG, and DxxxD motifs. In bacterial polymorphic toxin systems, the toxin is exported by the type 2, type 6 or type 7 secretion systems.


:

Pssm-ID: 464773  Cd Length: 94  Bit Score: 117.07  E-value: 1.52e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 970310189  212 WYQTVKTGSTWDHKPKIRDRFSSvavarplprqgkplrsYYHKYKQHDYFYDVWSNIHYGYVGLSVGFSESLLLKGSTWE 291
Cdd:pfam15607   2 WYEMVKTGGPWDHKPKIEDWLNT----------------IGHKYNGHDYFYDDWGNIHYGYVGRAAGFSESTLLRGSGLE 65

                          90       100       110
                  ....*....|....*....|....*....|
gi 970310189  292 QNMTPGAI--GDDTvDDVTSMKIGFALFHQ 319
Cdd:pfam15607  66 QAITNISNsfGDDP-DDKITIKIGIDLYDE 94
PAAR_CT_2 cd14744
proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This ...
 7-85 2.33e-20

proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly beta- and gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Most members contain C-terminal domain extensions corresponding to several uncharacterized domains such as S-type pyocin, DUF2235, DUF2345 and cytotoxic proteins. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


:

Pssm-ID: 269829  Cd Length: 78  Bit Score: 84.14  E-value: 2.33e-20
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 970310189   7 YLVQGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKFVGLYKVAGGIDNDTIHGRRMAGTLDsysTCPCKAKFIPS 85
Cdd:cd14744    3 IIRLGDKTTHGGVVISGSSTFTIDGRPVARVGDKVTCPKCKGTGPIVEGGPTFTVDGRPVALDGD---RVACGCPLIPS 78
 
Name Accession Description Interval E-value
Ntox44 pfam15607
Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The ...
 212-319 1.52e-32

Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The toxin possesses an all-alpha-helical fold with conserved DxK, GNxxxG, and DxxxD motifs. In bacterial polymorphic toxin systems, the toxin is exported by the type 2, type 6 or type 7 secretion systems.


Pssm-ID: 464773  Cd Length: 94  Bit Score: 117.07  E-value: 1.52e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 970310189  212 WYQTVKTGSTWDHKPKIRDRFSSvavarplprqgkplrsYYHKYKQHDYFYDVWSNIHYGYVGLSVGFSESLLLKGSTWE 291
Cdd:pfam15607   2 WYEMVKTGGPWDHKPKIEDWLNT----------------IGHKYNGHDYFYDDWGNIHYGYVGRAAGFSESTLLRGSGLE 65

                          90       100       110
                  ....*....|....*....|....*....|
gi 970310189  292 QNMTPGAI--GDDTvDDVTSMKIGFALFHQ 319
Cdd:pfam15607  66 QAITNISNsfGDDP-DDKITIKIGIDLYDE 94
PAAR_CT_2 cd14744
proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This ...
 7-85 2.33e-20

proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly beta- and gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Most members contain C-terminal domain extensions corresponding to several uncharacterized domains such as S-type pyocin, DUF2235, DUF2345 and cytotoxic proteins. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269829  Cd Length: 78  Bit Score: 84.14  E-value: 2.33e-20
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 970310189   7 YLVQGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKFVGLYKVAGGIDNDTIHGRRMAGTLDsysTCPCKAKFIPS 85
Cdd:cd14744    3 IIRLGDKTTHGGVVISGSSTFTIDGRPVARVGDKVTCPKCKGTGPIVEGGPTFTVDGRPVALDGD---RVACGCPLIPS 78
PAAR COG4104
Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular ...
 9-85 4.54e-12

Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443280  Cd Length: 87  Bit Score: 61.37  E-value: 4.54e-12
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 970310189   9 VQGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKfVGLYKVAGGIDNDTIHGRRMAGTLDSYStcpCKAKFIPS 85
Cdd:COG4104    7 RLGDKTSHGGPVISGSPTVLIGGRPAARVGDKVSCPK-HGPDTIAEGSPTVLINGKPAARVGDKTA---CGGTIISG 79
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 11-67 1.51e-09

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 53.73  E-value: 1.51e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 970310189   11 GDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKFVGLYKVAGGIDNDTIHGRRMA 67
Cdd:pfam05488   2 GDKTSHGGVVITGSPTVLIGGKPAARVGDLVVCPPCGGGGPIAEGSPTVLINGKPAA 58
 
Name Accession Description Interval E-value
Ntox44 pfam15607
Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The ...
 212-319 1.52e-32

Bacterial toxin 44; A predicted RNase toxin found in bacterial polymorphic toxin systems. The toxin possesses an all-alpha-helical fold with conserved DxK, GNxxxG, and DxxxD motifs. In bacterial polymorphic toxin systems, the toxin is exported by the type 2, type 6 or type 7 secretion systems.


Pssm-ID: 464773  Cd Length: 94  Bit Score: 117.07  E-value: 1.52e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 970310189  212 WYQTVKTGSTWDHKPKIRDRFSSvavarplprqgkplrsYYHKYKQHDYFYDVWSNIHYGYVGLSVGFSESLLLKGSTWE 291
Cdd:pfam15607   2 WYEMVKTGGPWDHKPKIEDWLNT----------------IGHKYNGHDYFYDDWGNIHYGYVGRAAGFSESTLLRGSGLE 65

                          90       100       110
                  ....*....|....*....|....*....|
gi 970310189  292 QNMTPGAI--GDDTvDDVTSMKIGFALFHQ 319
Cdd:pfam15607  66 QAITNISNsfGDDP-DDKITIKIGIDLYDE 94
PAAR_CT_2 cd14744
proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This ...
 7-85 2.33e-20

proline-alanine-alanine-arginine (PAAR) domain with uncharacterized C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly beta- and gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Most members contain C-terminal domain extensions corresponding to several uncharacterized domains such as S-type pyocin, DUF2235, DUF2345 and cytotoxic proteins. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269829  Cd Length: 78  Bit Score: 84.14  E-value: 2.33e-20
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 970310189   7 YLVQGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKFVGLYKVAGGIDNDTIHGRRMAGTLDsysTCPCKAKFIPS 85
Cdd:cd14744    3 IIRLGDKTTHGGVVISGSSTFTIDGRPVARVGDKVTCPKCKGTGPIVEGGPTFTVDGRPVALDGD---RVACGCPLIPS 78
PAAR COG4104
Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular ...
 9-85 4.54e-12

Zn-binding Pro-Ala-Ala-Arg (PAAR) domain, involved in Type VI secretion [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443280  Cd Length: 87  Bit Score: 61.37  E-value: 4.54e-12
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 970310189   9 VQGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKfVGLYKVAGGIDNDTIHGRRMAGTLDSYStcpCKAKFIPS 85
Cdd:COG4104    7 RLGDKTSHGGPVISGSPTVLIGGRPAARVGDKVSCPK-HGPDTIAEGSPTVLINGKPAARVGDKTA---CGGTIISG 79
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 11-67 1.51e-09

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 53.73  E-value: 1.51e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 970310189   11 GDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCGKFVGLYKVAGGIDNDTIHGRRMA 67
Cdd:pfam05488   2 GDKTSHGGVVITGSPTVLIGGKPAARVGDLVVCPPCGGGGPIAEGSPTVLINGKPAA 58
PAAR_motif pfam05488
PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. ...
 9-44 7.38e-04

PAAR motif; This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.


Pssm-ID: 428491  Cd Length: 71  Bit Score: 37.55  E-value: 7.38e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 970310189    9 VQGDKTTC-----GGRIITGAEDHTLFSKPVAREQDGVTCG 44
Cdd:pfam05488  27 RVGDLVVCppcggGGPIAEGSPTVLINGKPAAREGDKTACG 67
PAAR_CT_1 cd14743
proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found ...
 10-44 2.96e-03

proline-alanine-alanine-arginine (PAAR) domain with C-terminal extension; This domain is found in the PAAR (proline-alanine-alanine-arginine) repeat family of mostly gamma-proteobacteria, and forms a sharp conical extension on the VgrG spike, a trimeric protein complex of the bacterial type VI secretion system (T6SS). Some members contains C-terminal domain extensions corresponding to Rearrangement hotspot (Rhs) protein repeats and conserved Rhs repeat-associated unique core sequences as well as uncharacterized domains. However, these terminal domains are exposed to solution, and do not distort the binding site of VgrG. Rhs and related YD-peptide repeat proteins are widely distributed in bacteria. Rhs shares similar architecture with distantly related WapA proteins of Bacillus and Listeria species, suggesting intercellular growth inhibition as its primary function. Additionally, a plasmid-encoded Rhs protein has been implicated in bacteriocin production in Pseudomonas savastanoi. The pointed tip of the PAAR domain is stabilized by a zinc atom positioned close to the cone's vertex and is likely to be important for its integrity during penetration of the target cell envelope. VgrG proteins are orthologous to the central baseplate spikes of bacteriophages with contractile tails, and genes encoding proteins with PAAR motifs have been frequently found immediately downstream from vgrG-like genes.


Pssm-ID: 269828  Cd Length: 78  Bit Score: 36.13  E-value: 2.96e-03
                         10        20        30
                 ....*....|....*....|....*....|....*
gi 970310189  10 QGDKTTCGGRIITGAEDHTLFSKPVAREQDGVTCG 44
Cdd:cd14743   37 VGDKTSCGATIVSGSINVLINGKPAAVLGSTTSHG 71
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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