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Conserved domains on  [gi|992059442|ref|WP_061032595|]
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MULTISPECIES: tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO [Vibrio]

Protein Classification

tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO( domain architecture ID 14304487)

tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO is an S-adenosyl-L-methionine (SAM)-dependent methyltransferase that methylates position 37 (m(6)t(6)A37) to form N6-methyl-N6-threonylcarbamoyladenosine of the tRNA anticodon loop of tRNA(Thr)(GGU) that read codons starting with adenosine

EC:  2.1.1.-
Gene Symbol:  tsaA
Gene Ontology:  GO:0089715|GO:1904047|GO:0008168
PubMed:  22905870|12504684

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmO COG1720
tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA ...
 1-156 3.06e-73

tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA (Thr-GGU) A37 N6-methylase is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 441326  Cd Length: 152  Bit Score: 219.24  E-value: 3.06e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   1 MYSIEPIGFIESPYKEKFAVPRQPRLVPTSTSKVKLVDAanCLESVRNIEQFSHVWLLFLFDKNleAGWKPTVRPpRLGG 80
Cdd:COG1720    1 EITLRPIGVIRSPFKEKFGIPRQPRLAPGAEGEIELFPE--YAEALRGLEGFSHLWLLYWFHRA--RRWRLTVRP-RLGG 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 992059442  81 NERIGVFASRATFRPNGIGMSAVELKGVSQdkgqTWLDLGSVDLVDGTPIIDIKPYIPYSDSIPDAQGGFAAEEPE 156
Cdd:COG1720   76 NERRGVFATRSPHRPNPIGLSVVRLLGVEG----NRLHVRGLDLLDGTPVLDIKPYVPYADAIPDARSGWAEEAPE 147
TrmO_C pfam18389
TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase ...
 156-219 2.25e-23

TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase proteins. This domain has a RelE fold.


:

Pssm-ID: 465738  Cd Length: 65  Bit Score: 89.20  E-value: 2.25e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 992059442  156 EVLEVNFSQQAQSKLAQHPEA-RHIIQVIKEVLGQDPRPAYKKGKPDNKEYAVNLFDLNVKFVVE 219
Cdd:pfam18389   1 APLEVEFSEEAEAQLAALQRQyPDLRELIEQVLAQDPRPAYQKDKPDERVYGMRLYDFNIRWRVE 65
 
Name Accession Description Interval E-value
TrmO COG1720
tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA ...
 1-156 3.06e-73

tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA (Thr-GGU) A37 N6-methylase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441326  Cd Length: 152  Bit Score: 219.24  E-value: 3.06e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   1 MYSIEPIGFIESPYKEKFAVPRQPRLVPTSTSKVKLVDAanCLESVRNIEQFSHVWLLFLFDKNleAGWKPTVRPpRLGG 80
Cdd:COG1720    1 EITLRPIGVIRSPFKEKFGIPRQPRLAPGAEGEIELFPE--YAEALRGLEGFSHLWLLYWFHRA--RRWRLTVRP-RLGG 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 992059442  81 NERIGVFASRATFRPNGIGMSAVELKGVSQdkgqTWLDLGSVDLVDGTPIIDIKPYIPYSDSIPDAQGGFAAEEPE 156
Cdd:COG1720   76 NERRGVFATRSPHRPNPIGLSVVRLLGVEG----NRLHVRGLDLLDGTPVLDIKPYVPYADAIPDARSGWAEEAPE 147
TrmO pfam01980
tRNA-methyltransferase O; This family includes members such as TrmO (tRNA-methyltransferase O) ...
 19-141 2.15e-60

tRNA-methyltransferase O; This family includes members such as TrmO (tRNA-methyltransferase O) also known as YaeB, which contains a single-sheeted beta-barrel structure. TrmO is an AdoMet-dependent methyltransferase responsible for m6t6A formation. Its human homolog, is responsible for formation of m6t6A37 in cytoplasmic tRNASer. Lack of TrmO decreases attenuation activity of the thr operon, indicating that N6 methylation of m6t6A37 ensures efficient decoding of ACY codons. In bacteria and eukaryotes, TrmO has a C-terminal domain containing the conserved DPRxxY motif. Where the Asp194 and Arg196 in this motif of E. coli TrmO are necessary for N6-methylation. However, no archaeal YaeB has a C-terminal domain containing the DPRxxY motif that is conserved in bacterial and mammalian TrmO homologs.


Pssm-ID: 460402  Cd Length: 116  Bit Score: 185.29  E-value: 2.15e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   19 AVPRQPRLVPTSTSKVKLVDAANCLESVRNIEQFSHVWLLFLFDKNLEagwKPTVRPPRLGGNERIGVFASRATFRPNGI 98
Cdd:pfam01980   1 GIPRQPGLAPDARGTIELFPEYNNAEALRGLEGFSHLWLLYWFHRNTG---RPLVRPPRLGGNERVGVFATRSPFRPNPI 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 992059442   99 GMSAVELKGVsqdKGQTwLDLGSVDLVDGTPIIDIKPYIPYSD 141
Cdd:pfam01980  78 GLSVVKLLRV---EGNV-LHVSGVDLLDGTPVLDIKPYVPYAD 116
tRNA_TsaA TIGR00104
tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA; This protein has been characterized by ...
 1-148 3.21e-55

tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA; This protein has been characterized by crystallography in complex with S-Adenosylmethionine, making it a probable S-adenosylmethionine-dependent methyltransferase. Analysis in EcoGene links this protein to the enzyme characterization mapped to the tsaA gene in Escherichia coli. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 129210  Cd Length: 142  Bit Score: 173.04  E-value: 3.21e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442    1 MYSIEPIGFIESPYKEKFAVPRQPRlVPTSTSKVKLVDAAncLESVRNIEQFSHVWLLFLFDKNLEAGWKPTVRPPRLGG 80
Cdd:TIGR00104   2 DLQLKPIGVIRSPYKEKFDAPRQGR-FSDEVGEIEIFPEY--ADAVRGLEQFSHLIVLYWFDQAQRGKWQLTVVPPRLGG 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 992059442   81 NERIGVFASRATFRPNGIGMSAVELKGVSQDKgqtwLDLGSVDLVDGTPIIDIKPYIPYSDSIPDAQG 148
Cdd:TIGR00104  79 NQRRGVFATRSPHRPNPIGLSVVELLEVECNI----LKVGGLDALDGTPVIDIKPYSEYIDSVPNAQS 142
UPF0066 cd09281
Escherichia coli YaeB and related proteins; Uncharacterized protein family UPF0066. This ...
 6-136 3.08e-45

Escherichia coli YaeB and related proteins; Uncharacterized protein family UPF0066. This domain includes Escherichia coli YeaB, Archeoglobus fulgidus AF0241, and Agrobacterium tumefaciens VirR. Proteins with this domain are probable S-adenosylmethionine-dependent methyltransferases but they have not been functionally characterized and the substrate is unknown.


Pssm-ID: 187753  Cd Length: 124  Bit Score: 146.87  E-value: 3.08e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   6 PIGFIESPYKEKFAVPrQPRLVPTSTSKVKLVDAAncLESVRNIEQFSHVWLLFLFDKNLEAGWKPTVRPPRLGGNERIG 85
Cdd:cd09281    1 PIGVVRSPFKERFGAP-QQGLVGGVESEIEIDPEY--AEGLKGLEEFSHIWVLYWFHKADREELRVTVRPPPRGDNPKVG 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 992059442  86 VFASRATFRPNGIGMSAVELKGVSQDKgqtwLDLGSVDLVDGTPIIDIKPY 136
Cdd:cd09281   78 VFATRSPHRPNPIGLSVVRLLKVEGNR----LHVSGLDALDGTPVLDIKPY 124
TrmO_C pfam18389
TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase ...
 156-219 2.25e-23

TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase proteins. This domain has a RelE fold.


Pssm-ID: 465738  Cd Length: 65  Bit Score: 89.20  E-value: 2.25e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 992059442  156 EVLEVNFSQQAQSKLAQHPEA-RHIIQVIKEVLGQDPRPAYKKGKPDNKEYAVNLFDLNVKFVVE 219
Cdd:pfam18389   1 APLEVEFSEEAEAQLAALQRQyPDLRELIEQVLAQDPRPAYQKDKPDERVYGMRLYDFNIRWRVE 65
 
Name Accession Description Interval E-value
TrmO COG1720
tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA ...
 1-156 3.06e-73

tRNA (Thr-GGU) A37 N6-methylase [Translation, ribosomal structure and biogenesis]; tRNA (Thr-GGU) A37 N6-methylase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441326  Cd Length: 152  Bit Score: 219.24  E-value: 3.06e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   1 MYSIEPIGFIESPYKEKFAVPRQPRLVPTSTSKVKLVDAanCLESVRNIEQFSHVWLLFLFDKNleAGWKPTVRPpRLGG 80
Cdd:COG1720    1 EITLRPIGVIRSPFKEKFGIPRQPRLAPGAEGEIELFPE--YAEALRGLEGFSHLWLLYWFHRA--RRWRLTVRP-RLGG 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 992059442  81 NERIGVFASRATFRPNGIGMSAVELKGVSQdkgqTWLDLGSVDLVDGTPIIDIKPYIPYSDSIPDAQGGFAAEEPE 156
Cdd:COG1720   76 NERRGVFATRSPHRPNPIGLSVVRLLGVEG----NRLHVRGLDLLDGTPVLDIKPYVPYADAIPDARSGWAEEAPE 147
TrmO pfam01980
tRNA-methyltransferase O; This family includes members such as TrmO (tRNA-methyltransferase O) ...
 19-141 2.15e-60

tRNA-methyltransferase O; This family includes members such as TrmO (tRNA-methyltransferase O) also known as YaeB, which contains a single-sheeted beta-barrel structure. TrmO is an AdoMet-dependent methyltransferase responsible for m6t6A formation. Its human homolog, is responsible for formation of m6t6A37 in cytoplasmic tRNASer. Lack of TrmO decreases attenuation activity of the thr operon, indicating that N6 methylation of m6t6A37 ensures efficient decoding of ACY codons. In bacteria and eukaryotes, TrmO has a C-terminal domain containing the conserved DPRxxY motif. Where the Asp194 and Arg196 in this motif of E. coli TrmO are necessary for N6-methylation. However, no archaeal YaeB has a C-terminal domain containing the DPRxxY motif that is conserved in bacterial and mammalian TrmO homologs.


Pssm-ID: 460402  Cd Length: 116  Bit Score: 185.29  E-value: 2.15e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   19 AVPRQPRLVPTSTSKVKLVDAANCLESVRNIEQFSHVWLLFLFDKNLEagwKPTVRPPRLGGNERIGVFASRATFRPNGI 98
Cdd:pfam01980   1 GIPRQPGLAPDARGTIELFPEYNNAEALRGLEGFSHLWLLYWFHRNTG---RPLVRPPRLGGNERVGVFATRSPFRPNPI 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 992059442   99 GMSAVELKGVsqdKGQTwLDLGSVDLVDGTPIIDIKPYIPYSD 141
Cdd:pfam01980  78 GLSVVKLLRV---EGNV-LHVSGVDLLDGTPVLDIKPYVPYAD 116
tRNA_TsaA TIGR00104
tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA; This protein has been characterized by ...
 1-148 3.21e-55

tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA; This protein has been characterized by crystallography in complex with S-Adenosylmethionine, making it a probable S-adenosylmethionine-dependent methyltransferase. Analysis in EcoGene links this protein to the enzyme characterization mapped to the tsaA gene in Escherichia coli. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 129210  Cd Length: 142  Bit Score: 173.04  E-value: 3.21e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442    1 MYSIEPIGFIESPYKEKFAVPRQPRlVPTSTSKVKLVDAAncLESVRNIEQFSHVWLLFLFDKNLEAGWKPTVRPPRLGG 80
Cdd:TIGR00104   2 DLQLKPIGVIRSPYKEKFDAPRQGR-FSDEVGEIEIFPEY--ADAVRGLEQFSHLIVLYWFDQAQRGKWQLTVVPPRLGG 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 992059442   81 NERIGVFASRATFRPNGIGMSAVELKGVSQDKgqtwLDLGSVDLVDGTPIIDIKPYIPYSDSIPDAQG 148
Cdd:TIGR00104  79 NQRRGVFATRSPHRPNPIGLSVVELLEVECNI----LKVGGLDALDGTPVIDIKPYSEYIDSVPNAQS 142
UPF0066 cd09281
Escherichia coli YaeB and related proteins; Uncharacterized protein family UPF0066. This ...
 6-136 3.08e-45

Escherichia coli YaeB and related proteins; Uncharacterized protein family UPF0066. This domain includes Escherichia coli YeaB, Archeoglobus fulgidus AF0241, and Agrobacterium tumefaciens VirR. Proteins with this domain are probable S-adenosylmethionine-dependent methyltransferases but they have not been functionally characterized and the substrate is unknown.


Pssm-ID: 187753  Cd Length: 124  Bit Score: 146.87  E-value: 3.08e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 992059442   6 PIGFIESPYKEKFAVPrQPRLVPTSTSKVKLVDAAncLESVRNIEQFSHVWLLFLFDKNLEAGWKPTVRPPRLGGNERIG 85
Cdd:cd09281    1 PIGVVRSPFKERFGAP-QQGLVGGVESEIEIDPEY--AEGLKGLEEFSHIWVLYWFHKADREELRVTVRPPPRGDNPKVG 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 992059442  86 VFASRATFRPNGIGMSAVELKGVSQDKgqtwLDLGSVDLVDGTPIIDIKPY 136
Cdd:cd09281   78 VFATRSPHRPNPIGLSVVRLLKVEGNR----LHVSGLDALDGTPVLDIKPY 124
TrmO_C pfam18389
TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase ...
 156-219 2.25e-23

TrmO C-terminal domain; This domain is found at the C-terminus of TrmO tRNA methyltransferase proteins. This domain has a RelE fold.


Pssm-ID: 465738  Cd Length: 65  Bit Score: 89.20  E-value: 2.25e-23
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 992059442  156 EVLEVNFSQQAQSKLAQHPEA-RHIIQVIKEVLGQDPRPAYKKGKPDNKEYAVNLFDLNVKFVVE 219
Cdd:pfam18389   1 APLEVEFSEEAEAQLAALQRQyPDLRELIEQVLAQDPRPAYQKDKPDERVYGMRLYDFNIRWRVE 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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