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Conserved domains on  [gi|1000960180|ref|WP_061182538|]
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MULTISPECIES: restriction endonuclease [Bacillus cereus group]

Protein Classification

restriction endonuclease( domain architecture ID 11447960)

restriction endonuclease such as Escherichia coli Mrr restriction system protein (EcoKMrr), a type IV restriction endonuclease that recognizes and cleaves N6-methyladenine- and 5-methylcytosine-containing DNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
3-130 3.09e-13

Restriction endonuclease Mrr [Defense mechanisms];


:

Pssm-ID: 441321  Cd Length: 157  Bit Score: 66.12  E-value: 3.09e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1000960180   3 QEIEIIYNEHIAD--EKLKQ--GTKYERLAAIVFKTLNASDVVVHDLTlrGDGkktphQIDVMIT-RANVSKRVLIECKD 77
Cdd:COG1715     6 AAYEEIRAELAEEllERLRSlsPYEFEELVAELLRRMGYGSVEVTGRS--GDG-----GIDGIAKeDRLGGDRIYVQAKR 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1000960180  78 YDT-KVGISIIRDFFGAISQIKPDEAFVVTTEGYTKGARSFAEEEGIKLAILRG 130
Cdd:COG1715    79 YKGnTVGRPEVREFAGALAGEGADKGIFITTSRFTKDAREEARRLGRRIELIDG 132
 
Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
3-130 3.09e-13

Restriction endonuclease Mrr [Defense mechanisms];


Pssm-ID: 441321  Cd Length: 157  Bit Score: 66.12  E-value: 3.09e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1000960180   3 QEIEIIYNEHIAD--EKLKQ--GTKYERLAAIVFKTLNASDVVVHDLTlrGDGkktphQIDVMIT-RANVSKRVLIECKD 77
Cdd:COG1715     6 AAYEEIRAELAEEllERLRSlsPYEFEELVAELLRRMGYGSVEVTGRS--GDG-----GIDGIAKeDRLGGDRIYVQAKR 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1000960180  78 YDT-KVGISIIRDFFGAISQIKPDEAFVVTTEGYTKGARSFAEEEGIKLAILRG 130
Cdd:COG1715    79 YKGnTVGRPEVREFAGALAGEGADKGIFITTSRFTKDAREEARRLGRRIELIDG 132
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 21-125 2.78e-11

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 59.51  E-value: 2.78e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1000960180  21 GTKYERLAAIVFKTLNasdVVVHDLTLRGDGkktphQIDVMITRANVSK-RVLIECKDYDTKVGISIIRDFFGAISQIKP 99
Cdd:pfam04471   6 PKEFEELVAELLRAMG---YKVEVTGRSGDG-----GVDLIARKDPLGLeRILVQAKRYKNTVGVPAVQELVGALAGYGA 77

                          90       100
                  ....*....|....*....|....*..
gi 1000960180 100 DEAFVVTTEGYTKGARSFAEEE-GIKL 125
Cdd:pfam04471  78 DKGIFVTTSDFTPDAREFAKALnGIVL 104
 
Name Accession Description Interval E-value
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
3-130 3.09e-13

Restriction endonuclease Mrr [Defense mechanisms];


Pssm-ID: 441321  Cd Length: 157  Bit Score: 66.12  E-value: 3.09e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1000960180   3 QEIEIIYNEHIAD--EKLKQ--GTKYERLAAIVFKTLNASDVVVHDLTlrGDGkktphQIDVMIT-RANVSKRVLIECKD 77
Cdd:COG1715     6 AAYEEIRAELAEEllERLRSlsPYEFEELVAELLRRMGYGSVEVTGRS--GDG-----GIDGIAKeDRLGGDRIYVQAKR 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1000960180  78 YDT-KVGISIIRDFFGAISQIKPDEAFVVTTEGYTKGARSFAEEEGIKLAILRG 130
Cdd:COG1715    79 YKGnTVGRPEVREFAGALAGEGADKGIFITTSRFTKDAREEARRLGRRIELIDG 132
COG1787 COG1787
Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];
58-125 1.82e-11

Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];


Pssm-ID: 441393  Cd Length: 119  Bit Score: 60.27  E-value: 1.82e-11
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1000960180  58 IDVMITRANvsKRVLIECKDYDTKVGISIIRDFFGAISQIKPDEAFVVTTEGYTKGARSFAEEEGIKL 125
Cdd:COG1787    48 ADLIATKDG--ERIAVQCKRYKSPVGNKAVQEVVAAKAYYGADKAVVVTNSYFTKSAKELAKSNGVEL 113
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 21-125 2.78e-11

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 59.51  E-value: 2.78e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1000960180  21 GTKYERLAAIVFKTLNasdVVVHDLTLRGDGkktphQIDVMITRANVSK-RVLIECKDYDTKVGISIIRDFFGAISQIKP 99
Cdd:pfam04471   6 PKEFEELVAELLRAMG---YKVEVTGRSGDG-----GVDLIARKDPLGLeRILVQAKRYKNTVGVPAVQELVGALAGYGA 77

                          90       100
                  ....*....|....*....|....*..
gi 1000960180 100 DEAFVVTTEGYTKGARSFAEEE-GIKL 125
Cdd:pfam04471  78 DKGIFVTTSDFTPDAREFAKALnGIVL 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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