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Conserved domains on  [gi|1032590960|ref|WP_064221669|]
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ECP biosynthesis operon DNA-binding transcriptional regulator EcpR [Escherichia coli]

Protein Classification

helix-turn-helix transcriptional regulator( domain architecture ID 10006622)

helix-turn-helix (HTH) transcriptional regulator binds DNA and functions as a repressor or activator; similar to CsgD family transcriptional regulators which function in controlling the expression of structural components and assembly factors of curli fimbriae

Gene Ontology:  GO:0003700|GO:0003677|GO:0006355
PubMed:  20466769

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CsgD COG2771
DNA-binding transcriptional regulator, CsgD family [Transcription];
6-171 6.01e-10

DNA-binding transcriptional regulator, CsgD family [Transcription];


:

Pssm-ID: 442052 [Multi-domain]  Cd Length: 188  Bit Score: 55.92  E-value: 6.01e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960   6 RSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKDFVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEAL 85
Cdd:COG2771     1 LLLLALALLLLALLLLLLALLALLLLLLLAALLLLLALLLLAALLLLAAAAAALAAALAAALLLGLLLLLLIALLLLLLL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960  86 ANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDI----KKDKITDREMEIIRMTAQGIQPKSIARIENCSVKTVY 161
Cdd:COG2771    81 LLALLLLLALLALLAALLARLAALLLALALAALLLAALArllaRAPGLTPREREVLRLLAEGLTLKEIARILGISERTVR 160

                         170
                  ....*....|
gi 1032590960 162 THRRNAEAKL 171
Cdd:COG2771   161 THLKRIYRKL 170
 
Name Accession Description Interval E-value
CsgD COG2771
DNA-binding transcriptional regulator, CsgD family [Transcription];
6-171 6.01e-10

DNA-binding transcriptional regulator, CsgD family [Transcription];


Pssm-ID: 442052 [Multi-domain]  Cd Length: 188  Bit Score: 55.92  E-value: 6.01e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960   6 RSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKDFVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEAL 85
Cdd:COG2771     1 LLLLALALLLLALLLLLLALLALLLLLLLAALLLLLALLLLAALLLLAAAAAALAAALAAALLLGLLLLLLIALLLLLLL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960  86 ANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDI----KKDKITDREMEIIRMTAQGIQPKSIARIENCSVKTVY 161
Cdd:COG2771    81 LLALLLLLALLALLAALLARLAALLLALALAALLLAALArllaRAPGLTPREREVLRLLAEGLTLKEIARILGISERTVR 160

                         170
                  ....*....|
gi 1032590960 162 THRRNAEAKL 171
Cdd:COG2771   161 THLKRIYRKL 170
HTH_LUXR smart00421
helix_turn_helix, Lux Regulon; lux regulon (activates the bioluminescence operon
 130-171 1.75e-09

helix_turn_helix, Lux Regulon; lux regulon (activates the bioluminescence operon


Pssm-ID: 197715 [Multi-domain]  Cd Length: 58  Bit Score: 51.37  E-value: 1.75e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 1032590960  130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:smart00421   5 TPREREVLRLLAEGLTNKEIAERLGISEKTVKTHLSNIMRKL 46
LuxR_C_like cd06170
C-terminal DNA-binding domain of LuxR-like proteins. This domain contains a helix-turn-helix ...
 130-171 8.36e-09

C-terminal DNA-binding domain of LuxR-like proteins. This domain contains a helix-turn-helix motif and binds DNA. Proteins belonging to this group are response regulators; some act as transcriptional activators, others as transcriptional repressors. Many are active as homodimers. Many are two domain proteins in which the DNA binding property of the C-terminal DNA binding domain is modulated by modifications of the N-terminal domain. For example in the case of Lux R which participates in the regulation of gene expression in response to fluctuations in cell-population density (quorum-sensing), a signaling molecule, the pheromone Acyl HSL (N-acyl derivatives of homoserine lactone), binds to the N-terminal domain and leads to LuxR dimerization. For others phophorylation of the N-terminal domain leads to multimerization, for example Escherichia coli NarL and Sinorhizobium melilot FixJ. NarL controls gene expression of many respiratory-related operons when environmental nitrate or nitrite is present under anerobic conditions. FixJ is involved in the transcriptional activation of nitrogen fixation genes. The group also includes small proteins which lack an N-terminal signaling domain, such as Bacillus subtilis GerE. GerE is dimeric and acts in conjunction with sigmaK as an activator or a repressor modulating the expression of various genes in particular those encoding the spore-coat. These LuxR family regulators may share a similar organization of their target binding sites. For example the LuxR dimer binds the lux box, a 20bp inverted repeat, GerE dimers bind two 12bp consensus sequences in inverted orientation having the central four bases overlap, and the NarL dimer binds two 7bp inverted repeats separated by 2 bp.


Pssm-ID: 99777 [Multi-domain]  Cd Length: 57  Bit Score: 49.46  E-value: 8.36e-09
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:cd06170     2 TPREREVLRLLAEGKTNKEIADILGISEKTVKTHLRNIMRKL 43
GerE pfam00196
Bacterial regulatory proteins, luxR family;
130-171 1.37e-08

Bacterial regulatory proteins, luxR family;


Pssm-ID: 425517 [Multi-domain]  Cd Length: 57  Bit Score: 49.12  E-value: 1.37e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:pfam00196   4 SPREREVLRWLAAGKSNKEIADELGISEKTVKVHRSNIMRKL 45
PRK10360 PRK10360
transcriptional regulator UhpA;
70-171 2.65e-05

transcriptional regulator UhpA;


Pssm-ID: 182408 [Multi-domain]  Cd Length: 196  Bit Score: 42.66  E-value: 2.65e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960  70 KGKQVVLIAARKSEALANYwYYNSNIRGVVYAGLSRDirkELAYVIN-----GRFLRKDI-------KKDKITDREMEII 137
Cdd:PRK10360   71 KGMATIMLSVHDSPALVEQ-ALNAGARGFLSKRCSPD---ELIAAVHtvatgGCYLTPDIaiklasgRQDPLTKRERQVA 146

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1032590960 138 RMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:PRK10360  147 EKLAQGMAVKEIAAELGLSPKTVHVHRANLMEKL 180
 
Name Accession Description Interval E-value
CsgD COG2771
DNA-binding transcriptional regulator, CsgD family [Transcription];
6-171 6.01e-10

DNA-binding transcriptional regulator, CsgD family [Transcription];


Pssm-ID: 442052 [Multi-domain]  Cd Length: 188  Bit Score: 55.92  E-value: 6.01e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960   6 RSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKDFVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEAL 85
Cdd:COG2771     1 LLLLALALLLLALLLLLLALLALLLLLLLAALLLLLALLLLAALLLLAAAAAALAAALAAALLLGLLLLLLIALLLLLLL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960  86 ANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDI----KKDKITDREMEIIRMTAQGIQPKSIARIENCSVKTVY 161
Cdd:COG2771    81 LLALLLLLALLALLAALLARLAALLLALALAALLLAALArllaRAPGLTPREREVLRLLAEGLTLKEIARILGISERTVR 160

                         170
                  ....*....|
gi 1032590960 162 THRRNAEAKL 171
Cdd:COG2771   161 THLKRIYRKL 170
HTH_LUXR smart00421
helix_turn_helix, Lux Regulon; lux regulon (activates the bioluminescence operon
 130-171 1.75e-09

helix_turn_helix, Lux Regulon; lux regulon (activates the bioluminescence operon


Pssm-ID: 197715 [Multi-domain]  Cd Length: 58  Bit Score: 51.37  E-value: 1.75e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 1032590960  130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:smart00421   5 TPREREVLRLLAEGLTNKEIAERLGISEKTVKTHLSNIMRKL 46
LuxR_C_like cd06170
C-terminal DNA-binding domain of LuxR-like proteins. This domain contains a helix-turn-helix ...
 130-171 8.36e-09

C-terminal DNA-binding domain of LuxR-like proteins. This domain contains a helix-turn-helix motif and binds DNA. Proteins belonging to this group are response regulators; some act as transcriptional activators, others as transcriptional repressors. Many are active as homodimers. Many are two domain proteins in which the DNA binding property of the C-terminal DNA binding domain is modulated by modifications of the N-terminal domain. For example in the case of Lux R which participates in the regulation of gene expression in response to fluctuations in cell-population density (quorum-sensing), a signaling molecule, the pheromone Acyl HSL (N-acyl derivatives of homoserine lactone), binds to the N-terminal domain and leads to LuxR dimerization. For others phophorylation of the N-terminal domain leads to multimerization, for example Escherichia coli NarL and Sinorhizobium melilot FixJ. NarL controls gene expression of many respiratory-related operons when environmental nitrate or nitrite is present under anerobic conditions. FixJ is involved in the transcriptional activation of nitrogen fixation genes. The group also includes small proteins which lack an N-terminal signaling domain, such as Bacillus subtilis GerE. GerE is dimeric and acts in conjunction with sigmaK as an activator or a repressor modulating the expression of various genes in particular those encoding the spore-coat. These LuxR family regulators may share a similar organization of their target binding sites. For example the LuxR dimer binds the lux box, a 20bp inverted repeat, GerE dimers bind two 12bp consensus sequences in inverted orientation having the central four bases overlap, and the NarL dimer binds two 7bp inverted repeats separated by 2 bp.


Pssm-ID: 99777 [Multi-domain]  Cd Length: 57  Bit Score: 49.46  E-value: 8.36e-09
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:cd06170     2 TPREREVLRLLAEGKTNKEIADILGISEKTVKTHLRNIMRKL 43
GerE pfam00196
Bacterial regulatory proteins, luxR family;
130-171 1.37e-08

Bacterial regulatory proteins, luxR family;


Pssm-ID: 425517 [Multi-domain]  Cd Length: 57  Bit Score: 49.12  E-value: 1.37e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:pfam00196   4 SPREREVLRWLAAGKSNKEIADELGISEKTVKVHRSNIMRKL 45
CitB COG2197
DNA-binding response regulator, NarL/FixJ family, contains REC and HTH domains [Signal ...
 130-171 5.45e-08

DNA-binding response regulator, NarL/FixJ family, contains REC and HTH domains [Signal transduction mechanisms, Transcription];


Pssm-ID: 441799 [Multi-domain]  Cd Length: 131  Bit Score: 49.12  E-value: 5.45e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:COG2197    71 TPREREVLRLLAEGLSNKEIAERLGISERTVKTHVSNILRKL 112
GerE COG5905
Spore transcriptional regulator GerE (stand-alone HTH domain) [Cell cycle control, cell ...
 126-171 1.62e-05

Spore transcriptional regulator GerE (stand-alone HTH domain) [Cell cycle control, cell division, chromosome partitioning, Transcription];


Pssm-ID: 444607 [Multi-domain]  Cd Length: 76  Bit Score: 41.10  E-value: 1.62e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1032590960 126 KDKITDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:COG5905    10 PSLLTKREREVLELLAEGLTNKEIARQLFISEKTVKNHVSNILRKL 55
PRK10360 PRK10360
transcriptional regulator UhpA;
70-171 2.65e-05

transcriptional regulator UhpA;


Pssm-ID: 182408 [Multi-domain]  Cd Length: 196  Bit Score: 42.66  E-value: 2.65e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032590960  70 KGKQVVLIAARKSEALANYwYYNSNIRGVVYAGLSRDirkELAYVIN-----GRFLRKDI-------KKDKITDREMEII 137
Cdd:PRK10360   71 KGMATIMLSVHDSPALVEQ-ALNAGARGFLSKRCSPD---ELIAAVHtvatgGCYLTPDIaiklasgRQDPLTKRERQVA 146

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1032590960 138 RMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:PRK10360  147 EKLAQGMAVKEIAAELGLSPKTVHVHRANLMEKL 180
FixJ COG4566
DNA-binding response regulator, FixJ family, consists of REC and HTH domains [Signal ...
 130-171 2.64e-03

DNA-binding response regulator, FixJ family, consists of REC and HTH domains [Signal transduction mechanisms, Transcription];


Pssm-ID: 443623 [Multi-domain]  Cd Length: 196  Bit Score: 37.00  E-value: 2.64e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1032590960 130 TDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:COG4566   139 TPREREVLDLVVAGLSNKQIARELGISPRTVEVHRANVMEKL 180
PRK10403 PRK10403
nitrate/nitrite response regulator protein NarP;
129-171 4.30e-03

nitrate/nitrite response regulator protein NarP;


Pssm-ID: 182431 [Multi-domain]  Cd Length: 215  Bit Score: 36.37  E-value: 4.30e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1032590960 129 ITDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:PRK10403  154 LTERELDVLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKL 196
PRK15369 PRK15369
two component system response regulator;
129-171 8.96e-03

two component system response regulator;


Pssm-ID: 185267 [Multi-domain]  Cd Length: 211  Bit Score: 35.44  E-value: 8.96e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 1032590960 129 ITDREMEIIRMTAQGIQPKSIARIENCSVKTVYTHRRNAEAKL 171
Cdd:PRK15369  150 LTPRERQILKLITEGYTNRDIAEQLSISIKTVETHRLNMMRKL 192
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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