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Conserved domains on  [gi|1196966147|ref|WP_086389268|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11450351)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
9-140 1.14e-49

Predicted N-acyltransferase, GNAT family [General function prediction only];


:

Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 154.96  E-value: 1.14e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147   9 DEQLRDAFSVRKQVFVNEQHVSAEEEYDEFEETSTHVVIYDNDVPVGAGRFRILD-GIGKMERICVLNSHRKKGIGKIVM 87
Cdd:COG2153     1 AEELYDALALRREVFVVEQGVPPYLELDGKDEDARHLLAYDDGELVATARLLPPGdGEAKIGRVAVLPEYRGQGLGRALM 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1196966147  88 DSLEAYAKENSLPKLKLHAQTHAEDFYKKLGYVTSSDVFMEANIPHVVMIKEL 140
Cdd:COG2153    81 EAAIEEARERGARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKPL 133
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
9-140 1.14e-49

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 154.96  E-value: 1.14e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147   9 DEQLRDAFSVRKQVFVNEQHVSAEEEYDEFEETSTHVVIYDNDVPVGAGRFRILD-GIGKMERICVLNSHRKKGIGKIVM 87
Cdd:COG2153     1 AEELYDALALRREVFVVEQGVPPYLELDGKDEDARHLLAYDDGELVATARLLPPGdGEAKIGRVAVLPEYRGQGLGRALM 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1196966147  88 DSLEAYAKENSLPKLKLHAQTHAEDFYKKLGYVTSSDVFMEANIPHVVMIKEL 140
Cdd:COG2153    81 EAAIEEARERGARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKPL 133
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 43-140 8.51e-19

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 76.54  E-value: 8.51e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  43 THVVIYDNDVPVGAGRFRildGIGKMERICVLNSHRKKGIGKIVMDSLEAYAKEN--SLPKLKLHAQTHAEDFYKKLGYV 120
Cdd:pfam13673  32 FFFVAFEGGQIVGVIALR---DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDgiKLSELTVNASPYAVPFYEKLGFR 108

                          90       100
                  ....*....|....*....|
gi 1196966147 121 TSSDVFMEANIPHVVMIKEL 140
Cdd:pfam13673 109 ATGPEQEFNGIRFVPMEKEL 128
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-105 1.04e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.43  E-value: 1.04e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1196966147  44 HVVIYDNDVPVGAGRFRILDGIGK---MERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH 105
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSGGDtayIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10314 PRK10314
GNAT family N-acetyltransferase;
11-139 2.00e-06

GNAT family N-acetyltransferase;


Pssm-ID: 182373 [Multi-domain]  Cd Length: 153  Bit Score: 44.45  E-value: 2.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  11 QLRDAFSVRKQVFVNEQHVSAEEEYDEFEE-TSTHVVIYDNDVPVGAGRfrILDGIGKME-----RICVLNSHRKKGIGK 84
Cdd:PRK10314   16 QLYALLQLRCAVFVVEQNCPYQDIDGDDLTgDNRHILGWKNDELVAYAR--ILKSDDDLEpvvigRVIVSEALRGEKVGQ 93

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1196966147  85 IVMD-SLEAYAKENSLPKLKLHAQTHAEDFYKKLGYVTSSDVFMEANIPHVVMIKE 139
Cdd:PRK10314   94 QLMSkTLESCTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARE 149
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
9-140 1.14e-49

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 154.96  E-value: 1.14e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147   9 DEQLRDAFSVRKQVFVNEQHVSAEEEYDEFEETSTHVVIYDNDVPVGAGRFRILD-GIGKMERICVLNSHRKKGIGKIVM 87
Cdd:COG2153     1 AEELYDALALRREVFVVEQGVPPYLELDGKDEDARHLLAYDDGELVATARLLPPGdGEAKIGRVAVLPEYRGQGLGRALM 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1196966147  88 DSLEAYAKENSLPKLKLHAQTHAEDFYKKLGYVTSSDVFMEANIPHVVMIKEL 140
Cdd:COG2153    81 EAAIEEARERGARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKPL 133
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 43-140 8.51e-19

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 76.54  E-value: 8.51e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  43 THVVIYDNDVPVGAGRFRildGIGKMERICVLNSHRKKGIGKIVMDSLEAYAKEN--SLPKLKLHAQTHAEDFYKKLGYV 120
Cdd:pfam13673  32 FFFVAFEGGQIVGVIALR---DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDgiKLSELTVNASPYAVPFYEKLGFR 108

                          90       100
                  ....*....|....*....|
gi 1196966147 121 TSSDVFMEANIPHVVMIKEL 140
Cdd:pfam13673 109 ATGPEQEFNGIRFVPMEKEL 128
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 43-140 4.19e-17

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 72.39  E-value: 4.19e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  43 THVVIYDNDVPVGAGRFRILDG-IGKMERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH---AQTHAEDFYKKLG 118
Cdd:COG0454    35 EFIAVDDKGEPIGFAGLRRLDDkVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDtldGNPAAIRFYERLG 114

                          90       100
                  ....*....|....*....|..
gi 1196966147 119 YVTSSDVfmeANIPHVVMIKEL 140
Cdd:COG0454   115 FKEIERY---VAYVGGEFEKEL 133
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 42-120 3.44e-15

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 65.94  E-value: 3.44e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  42 STHVVIYDNDVPVGAGRFRILDGIGKME--RICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLHAQTHAEDFYKKLGY 119
Cdd:pfam13508   3 GRFFVAEDDGKIVGFAALLPLDDEGALAelRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKLGF 82


                  .
gi 1196966147 120 V 120
Cdd:pfam13508  83 E 83
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 45-140 4.22e-15

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 66.94  E-value: 4.22e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  45 VVIYDNDVPVGAGRFRILD-GIGKMERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLHAQTHAEDFYKKLGYV--- 120
Cdd:COG1246    31 WVAEEDGEIVGCAALHPLDeDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSAAIHFYEKLGFEeid 110

                          90       100
                  ....*....|....*....|.
gi 1196966147 121 -TSSDVFMEANIPHVVMIKEL 140
Cdd:COG1246   111 kEDLPYAKVWQRDSVVMEKDL 131
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
45-140 3.07e-14

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 65.11  E-value: 3.07e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  45 VVIYDNDVPVGAGRFRILDGIGK-----MERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLHAQTHAEDFYKKLGY 119
Cdd:COG3153    42 LVAEDDGEIVGHVALSPVDIDGEgpallLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPFYERFGF 121

                          90       100
                  ....*....|....*....|.
gi 1196966147 120 VTSSDVFMEANIPHVVMIKEL 140
Cdd:COG3153   122 RPAGELGLTLGPDEVFLAKEL 142
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 42-119 4.09e-14

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 64.08  E-value: 4.09e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  42 STHVVIYDNDVPVGAGRFRILD---GIGKMERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH---AQTHAEDFYK 115
Cdd:pfam00583  33 EGFFVAEEDGELVGFASLSIIDdepPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEvaaDNLAAIALYE 112


                  ....
gi 1196966147 116 KLGY 119
Cdd:pfam00583 113 KLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 56-140 4.80e-12

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 58.13  E-value: 4.80e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  56 AGRFRILDGIGKMERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLHAQTH---AEDFYKKLGYVTSSDVFMEANIP 132
Cdd:COG0456     4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDneaAIALYEKLGFEEVGERPNYYGDD 83


                  ....*...
gi 1196966147 133 HVVMIKEL 140
Cdd:COG0456    84 ALVMEKEL 91
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-105 1.04e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.43  E-value: 1.04e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1196966147  44 HVVIYDNDVPVGAGRFRILDGIGK---MERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH 105
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSGGDtayIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
53-127 5.88e-07

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 44.51  E-value: 5.88e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1196966147  53 PVGAGRFRI-LDGIGKMERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH---AQTHAEDFYKKLGYVTSSDVFM 127
Cdd:COG3393     2 LVAMAGVRAeSPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYvdaDNPAARRLYERLGFRPVGEYAT 80
PRK10314 PRK10314
GNAT family N-acetyltransferase;
11-139 2.00e-06

GNAT family N-acetyltransferase;


Pssm-ID: 182373 [Multi-domain]  Cd Length: 153  Bit Score: 44.45  E-value: 2.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  11 QLRDAFSVRKQVFVNEQHVSAEEEYDEFEE-TSTHVVIYDNDVPVGAGRfrILDGIGKME-----RICVLNSHRKKGIGK 84
Cdd:PRK10314   16 QLYALLQLRCAVFVVEQNCPYQDIDGDDLTgDNRHILGWKNDELVAYAR--ILKSDDDLEpvvigRVIVSEALRGEKVGQ 93

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1196966147  85 IVMD-SLEAYAKENSLPKLKLHAQTHAEDFYKKLGYVTSSDVFMEANIPHVVMIKE 139
Cdd:PRK10314   94 QLMSkTLESCTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARE 149
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
45-121 1.60e-05

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 42.29  E-value: 1.60e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1196966147  45 VVIYDNDVPVG---AGRFRILDGIGKM--ERICVLNSHRKKGIGKIVMDSLEAYAKENSLPKLKLH---AQTHAEDFYKK 116
Cdd:COG1247    55 LVAEEDGEVVGfasLGPFRPRPAYRGTaeESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVvlaDNEASIALYEK 134


                  ....*
gi 1196966147 117 LGYVT 121
Cdd:COG1247   135 LGFEE 139
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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