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Conserved domains on  [gi|1248243915|ref|WP_096739939|]
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MULTISPECIES: glutamate--cysteine ligase [Pseudoalteromonas]

Protein Classification

glutamate--cysteine ligase( domain architecture ID 10006790)

glutamate--cysteine ligase catalyzes the conversion of ATP, L-glutamate, and L-cysteine to ADP, phosphate, and gamma-L-glutamyl-L-cysteine

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
3-521 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


:

Pssm-ID: 442162  Cd Length: 518  Bit Score: 811.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915   3 IHDLSSALNALSATPHQHAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATET 82
Cdd:COG2918     2 SPDLSRRLAWLAAPAHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDEL 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  83 LAQLKDLQKFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETL 162
Cdd:COG2918    82 LAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEAF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 163 WQSLHTLKQSEQSLEAFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRKTdlPFKKLGKGTLYLEVGTALRLGD 242
Cdd:COG2918   162 WQALQGLEGDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMSD 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 243 LGYTNSAQSSLRVMYNSLDEYVAGLKKAINTPSDLYGDI-----DDYttatpKQLNKNILQIENEFYSPIRPKRNAKSGE 317
Cdd:COG2918   240 LGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIgvkvdGEY-----RQLNTNILQIENEYYSPIRPKRVTRSGE 314
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 318 KPTDALLRAGIEYVEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVNQGRQLGVMLND 397
Cdd:COG2918   315 KPSQALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQR 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 398 NNQQRTLQSWGGEIFDQLSEVAEHMDKAYGVTYYSETIAQMATWVDNPALTFSGRYVRELESAGIDNGLFAIQLANKYKQ 477
Cdd:COG2918   395 GGEEIPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGFGEFGLRLAKQHRE 474
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 1248243915 478 SHAQANYNVFSKEWLEQQVVLSDKAREEIERADTLSFTDFLDEY 521
Cdd:COG2918   475 YLRAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLARY 518
 
Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
3-521 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


Pssm-ID: 442162  Cd Length: 518  Bit Score: 811.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915   3 IHDLSSALNALSATPHQHAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATET 82
Cdd:COG2918     2 SPDLSRRLAWLAAPAHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDEL 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  83 LAQLKDLQKFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETL 162
Cdd:COG2918    82 LAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEAF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 163 WQSLHTLKQSEQSLEAFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRKTdlPFKKLGKGTLYLEVGTALRLGD 242
Cdd:COG2918   162 WQALQGLEGDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMSD 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 243 LGYTNSAQSSLRVMYNSLDEYVAGLKKAINTPSDLYGDI-----DDYttatpKQLNKNILQIENEFYSPIRPKRNAKSGE 317
Cdd:COG2918   240 LGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIgvkvdGEY-----RQLNTNILQIENEYYSPIRPKRVTRSGE 314
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 318 KPTDALLRAGIEYVEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVNQGRQLGVMLND 397
Cdd:COG2918   315 KPSQALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQR 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 398 NNQQRTLQSWGGEIFDQLSEVAEHMDKAYGVTYYSETIAQMATWVDNPALTFSGRYVRELESAGIDNGLFAIQLANKYKQ 477
Cdd:COG2918   395 GGEEIPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGFGEFGLRLAKQHRE 474
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 1248243915 478 SHAQANYNVFSKEWLEQQVVLSDKAREEIERADTLSFTDFLDEY 521
Cdd:COG2918   475 YLRAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLARY 518
Glu_cys_ligase pfam04262
Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that ...
11-384 0e+00

Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that carry out the first step of the glutathione biosynthesis pathway.


Pssm-ID: 427826  Cd Length: 371  Bit Score: 646.89  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  11 NALSATPHQHAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQLKDLQ 90
Cdd:pfam04262   1 ALLSDNPHLPLLKGILRGIERESLRVTPDGKLAQTPHPKALGSALTHPWITTDFSESLLEFITPPFSDVDETLDFLNDLH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  91 KFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSLHTLK 170
Cdd:pfam04262  81 RFVYRNLGDELLWPLSMPCVLPDEEDIPIAQYGTSNIGRMKTVYREGLAHRYGKKMQTIAGVHYNFSLPDSFWPLLQELE 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 171 QSEQSLEaFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRktDLPFKKLGKGTLYLEVGTALRLGDLGYTNSAQ 250
Cdd:pfam04262 161 GSELSQD-FISDGYFGLIRNFYRYGWLLPYLFGASPALCSSFLKGR--NHPLEKLDAGTLYLPYATSLRMSDLGYTNKAQ 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 251 SSLRVMYNSLDEYVAGLKKAINTPSDLYGDIDDYTTATPKQLNKNILQIENEFYSPIRPKRNAKSGEKPTDALLRAGIEY 330
Cdd:pfam04262 238 ASLKISYNSLDEYVKSLKKAISTPSPEYEKIGVKVDGEYRQLNTNVLQIENEFYSPIRPKRVTKSGERPLDALLRRGVEY 317
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1248243915 331 VEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTV 384
Cdd:pfam04262 318 IEVRSLDINPFSPLGIDEEQLRFLDLFLLYCLLEESPEIDEEELAEIRKNQERV 371
glu_cys_ligase TIGR01434
glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model ...
7-521 0e+00

glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model represents glutamate--cysteine ligase, and enzyme in the biosynthesis of glutathione (GSH). GSH is one of several low molecular weight cysteine derivatives that can serve to protect against oxidative damage and participate in a biosynthetic or detoxification reactions. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]


Pssm-ID: 213621  Cd Length: 512  Bit Score: 573.35  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915   7 SSALNALSATPHqhAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQL 86
Cdd:TIGR01434   1 SQALAWLEKNPQ--ALKGIQRGIERETLRVNADGTLATTGHPEALGSALTHKWITTDFSEALLEFITPTDGDIAHMLTFL 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  87 KDLQKFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSL 166
Cdd:TIGR01434  79 RDLHRFTASKLGDERMWPLSMPCYIADGQDIEIAQYGTSNIGRFKTLYREGLKNRYGALMQTISGIHYNFSLPMAFWQAK 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 167 HTLKQSEQSLEaFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRKTDLPFKKLGKGTLYLEVGTALRLGDLGYT 246
Cdd:TIGR01434 159 RGAEGGESAKD-KISAGYFRLIRNYYRFGWVIPYLFGASPAISSSFLQGKPTKLPFEKTDEGMYYLPYATSLRLSDLGYT 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 247 NSAQSSLRVMYNSLDEYVAGLKKAINTPSDLYGDIDDYTTATPKQLNKNILQIENEFYSPIRPKRNAKSGEKPTDALLRA 326
Cdd:TIGR01434 238 NKSQSNLGITFNDLQEYIASLKQAIKTPSEEYAKIGTKKDGKRLQLNTNILQIENELYAPIRPKRVTRSGESPSDALLRR 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 327 GIEYVEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVNQGRQLGVML--NDNNQQRTL 404
Cdd:TIGR01434 318 GIEYVEVRSLDINPFSPIGIDEQQVRFLDLFLVWCALADSPEMSSGELLCTTDNWNRVILEGRKPGLTLgiGCETAQFPL 397
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 405 QSWGGEIFDQLSEVAEHMDKAYGVTYYSETIAQMATWVDNPALTFSGRYVRELESAGIdnGLFAIQLANKYKQSHAQANY 484
Cdd:TIGR01434 398 PVVGKDLFRDLKRVAQTLDSIRGGEAYQKACDELVACFDNPELTFSAQILRSMIDTGI--GGTGLAFAEAYRELFREEPL 475
                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 1248243915 485 NVFSKEWLEQQVVLSDKAREEIERADTLSFTDFLDEY 521
Cdd:TIGR01434 476 EILEQERFEAERSASLRRQQEIERADTEPFATWLAKH 512
PRK02471 PRK02471
bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;
28-485 1.03e-98

bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;


Pssm-ID: 179427 [Multi-domain]  Cd Length: 752  Bit Score: 314.56  E-value: 1.03e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  28 GIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQLKDLQKFTLEHMG-DELLWPIS 106
Cdd:PRK02471   22 GLEKESLRVDSDGKLALTPHPKAFGSRSFHPYIQTDFSESQLELITPVADSIEEVYRFLGALHDVASRSLDeDEYLWPLS 101
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 107 MPCFIEDQDDIVLAQFGNsntaRMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSLHTLKQSEQSLEAFISDGYLA 186
Cdd:PRK02471  102 MPPILPDEEEIPIAQLDN----REDVQYREYLAKKYGKKKQMISGIHYNFSLGEALIQRLFEEQSEQQDYKDFKNDLYLK 177
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 187 LIRNFKRELWLISYLFGASPALCNSFLQGrKTDLPFKKlgkgtlylevGTALRLGDLGYTNSaqSSLRVMYNSLDEYVAG 266
Cdd:PRK02471  178 VARNFLRYRWLLTYLFGASPVAEADFFKQ-NKEYLNQD----------VRSLRNSKYGYVNK--EDVYVSYNSLEDYVSS 244
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 267 LKKAIntpsdlygdiddyttatpkqlNKNILQIENEFYSPIRPKrnaksGEKPTDALLRAGIEYVEIRALDVNPFSETGI 346
Cdd:PRK02471  245 LENAV---------------------ESGQLISEKEFYSPVRLR-----GAKQDRSLLEKGITYLEFRLFDLNPFEPYGI 298
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 347 DIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVnqgrqlgvmLNDNNQQRTLQSWGGEIFDQLSEVAEHmdkaY 426
Cdd:PRK02471  299 SQETLDFVHLFLLYLLWLDEPEDTDQWVELADENNNLIA---------LEHPLEALALAAEAELILDAMEDLVQH----L 365
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 427 GV-TYYSETIAQMATWVDNPALTFSGRYVRELESAGIDNglFAIQLANKYKQSHAQANYN 485
Cdd:PRK02471  366 GLpDEYQDLIKQLKEQLTDPEKTLSGRLLKQIKQESYVA--FGLEQAKQYKEYAWERPYA 423
 
Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
3-521 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


Pssm-ID: 442162  Cd Length: 518  Bit Score: 811.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915   3 IHDLSSALNALSATPHQHAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATET 82
Cdd:COG2918     2 SPDLSRRLAWLAAPAHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDEL 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  83 LAQLKDLQKFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETL 162
Cdd:COG2918    82 LAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEAF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 163 WQSLHTLKQSEQSLEAFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRKTdlPFKKLGKGTLYLEVGTALRLGD 242
Cdd:COG2918   162 WQALQGLEGDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMSD 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 243 LGYTNSAQSSLRVMYNSLDEYVAGLKKAINTPSDLYGDI-----DDYttatpKQLNKNILQIENEFYSPIRPKRNAKSGE 317
Cdd:COG2918   240 LGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIgvkvdGEY-----RQLNTNILQIENEYYSPIRPKRVTRSGE 314
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 318 KPTDALLRAGIEYVEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVNQGRQLGVMLND 397
Cdd:COG2918   315 KPSQALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQR 394
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 398 NNQQRTLQSWGGEIFDQLSEVAEHMDKAYGVTYYSETIAQMATWVDNPALTFSGRYVRELESAGIDNGLFAIQLANKYKQ 477
Cdd:COG2918   395 GGEEIPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGFGEFGLRLAKQHRE 474
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 1248243915 478 SHAQANYNVFSKEWLEQQVVLSDKAREEIERADTLSFTDFLDEY 521
Cdd:COG2918   475 YLRAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLARY 518
Glu_cys_ligase pfam04262
Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that ...
11-384 0e+00

Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that carry out the first step of the glutathione biosynthesis pathway.


Pssm-ID: 427826  Cd Length: 371  Bit Score: 646.89  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  11 NALSATPHQHAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQLKDLQ 90
Cdd:pfam04262   1 ALLSDNPHLPLLKGILRGIERESLRVTPDGKLAQTPHPKALGSALTHPWITTDFSESLLEFITPPFSDVDETLDFLNDLH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  91 KFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSLHTLK 170
Cdd:pfam04262  81 RFVYRNLGDELLWPLSMPCVLPDEEDIPIAQYGTSNIGRMKTVYREGLAHRYGKKMQTIAGVHYNFSLPDSFWPLLQELE 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 171 QSEQSLEaFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRktDLPFKKLGKGTLYLEVGTALRLGDLGYTNSAQ 250
Cdd:pfam04262 161 GSELSQD-FISDGYFGLIRNFYRYGWLLPYLFGASPALCSSFLKGR--NHPLEKLDAGTLYLPYATSLRMSDLGYTNKAQ 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 251 SSLRVMYNSLDEYVAGLKKAINTPSDLYGDIDDYTTATPKQLNKNILQIENEFYSPIRPKRNAKSGEKPTDALLRAGIEY 330
Cdd:pfam04262 238 ASLKISYNSLDEYVKSLKKAISTPSPEYEKIGVKVDGEYRQLNTNVLQIENEFYSPIRPKRVTKSGERPLDALLRRGVEY 317
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1248243915 331 VEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTV 384
Cdd:pfam04262 318 IEVRSLDINPFSPLGIDEEQLRFLDLFLLYCLLEESPEIDEEELAEIRKNQERV 371
glu_cys_ligase TIGR01434
glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model ...
7-521 0e+00

glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model represents glutamate--cysteine ligase, and enzyme in the biosynthesis of glutathione (GSH). GSH is one of several low molecular weight cysteine derivatives that can serve to protect against oxidative damage and participate in a biosynthetic or detoxification reactions. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]


Pssm-ID: 213621  Cd Length: 512  Bit Score: 573.35  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915   7 SSALNALSATPHqhAIKGIKRGIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQL 86
Cdd:TIGR01434   1 SQALAWLEKNPQ--ALKGIQRGIERETLRVNADGTLATTGHPEALGSALTHKWITTDFSEALLEFITPTDGDIAHMLTFL 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  87 KDLQKFTLEHMGDELLWPISMPCFIEDQDDIVLAQFGNSNTARMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSL 166
Cdd:TIGR01434  79 RDLHRFTASKLGDERMWPLSMPCYIADGQDIEIAQYGTSNIGRFKTLYREGLKNRYGALMQTISGIHYNFSLPMAFWQAK 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 167 HTLKQSEQSLEaFISDGYLALIRNFKRELWLISYLFGASPALCNSFLQGRKTDLPFKKLGKGTLYLEVGTALRLGDLGYT 246
Cdd:TIGR01434 159 RGAEGGESAKD-KISAGYFRLIRNYYRFGWVIPYLFGASPAISSSFLQGKPTKLPFEKTDEGMYYLPYATSLRLSDLGYT 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 247 NSAQSSLRVMYNSLDEYVAGLKKAINTPSDLYGDIDDYTTATPKQLNKNILQIENEFYSPIRPKRNAKSGEKPTDALLRA 326
Cdd:TIGR01434 238 NKSQSNLGITFNDLQEYIASLKQAIKTPSEEYAKIGTKKDGKRLQLNTNILQIENELYAPIRPKRVTRSGESPSDALLRR 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 327 GIEYVEIRALDVNPFSETGIDIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVNQGRQLGVML--NDNNQQRTL 404
Cdd:TIGR01434 318 GIEYVEVRSLDINPFSPIGIDEQQVRFLDLFLVWCALADSPEMSSGELLCTTDNWNRVILEGRKPGLTLgiGCETAQFPL 397
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 405 QSWGGEIFDQLSEVAEHMDKAYGVTYYSETIAQMATWVDNPALTFSGRYVRELESAGIdnGLFAIQLANKYKQSHAQANY 484
Cdd:TIGR01434 398 PVVGKDLFRDLKRVAQTLDSIRGGEAYQKACDELVACFDNPELTFSAQILRSMIDTGI--GGTGLAFAEAYRELFREEPL 475
                         490       500       510
                  ....*....|....*....|....*....|....*..
gi 1248243915 485 NVFSKEWLEQQVVLSDKAREEIERADTLSFTDFLDEY 521
Cdd:TIGR01434 476 EILEQERFEAERSASLRRQQEIERADTEPFATWLAKH 512
PRK02471 PRK02471
bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;
28-485 1.03e-98

bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;


Pssm-ID: 179427 [Multi-domain]  Cd Length: 752  Bit Score: 314.56  E-value: 1.03e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915  28 GIERESLRIKGNGAISTLGHPKGVGSALTNGHITTDFSESLLEFITPVSESATETLAQLKDLQKFTLEHMG-DELLWPIS 106
Cdd:PRK02471   22 GLEKESLRVDSDGKLALTPHPKAFGSRSFHPYIQTDFSESQLELITPVADSIEEVYRFLGALHDVASRSLDeDEYLWPLS 101
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 107 MPCFIEDQDDIVLAQFGNsntaRMKTLYREGLKNRYGSMMQAIAGVHFNISFPETLWQSLHTLKQSEQSLEAFISDGYLA 186
Cdd:PRK02471  102 MPPILPDEEEIPIAQLDN----REDVQYREYLAKKYGKKKQMISGIHYNFSLGEALIQRLFEEQSEQQDYKDFKNDLYLK 177
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 187 LIRNFKRELWLISYLFGASPALCNSFLQGrKTDLPFKKlgkgtlylevGTALRLGDLGYTNSaqSSLRVMYNSLDEYVAG 266
Cdd:PRK02471  178 VARNFLRYRWLLTYLFGASPVAEADFFKQ-NKEYLNQD----------VRSLRNSKYGYVNK--EDVYVSYNSLEDYVSS 244
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 267 LKKAIntpsdlygdiddyttatpkqlNKNILQIENEFYSPIRPKrnaksGEKPTDALLRAGIEYVEIRALDVNPFSETGI 346
Cdd:PRK02471  245 LENAV---------------------ESGQLISEKEFYSPVRLR-----GAKQDRSLLEKGITYLEFRLFDLNPFEPYGI 298
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 347 DIEQIHFLDVFLTYCLLKDSPEMQWKEQARSTENLDTVVnqgrqlgvmLNDNNQQRTLQSWGGEIFDQLSEVAEHmdkaY 426
Cdd:PRK02471  299 SQETLDFVHLFLLYLLWLDEPEDTDQWVELADENNNLIA---------LEHPLEALALAAEAELILDAMEDLVQH----L 365
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1248243915 427 GV-TYYSETIAQMATWVDNPALTFSGRYVRELESAGIDNglFAIQLANKYKQSHAQANYN 485
Cdd:PRK02471  366 GLpDEYQDLIKQLKEQLTDPEKTLSGRLLKQIKQESYVA--FGLEQAKQYKEYAWERPYA 423
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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