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Conserved domains on  [gi|1267774385|ref|WP_098519290|]
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restriction endonuclease [Bacillus thuringiensis]

Protein Classification

restriction endonuclease( domain architecture ID 11448432)

DNA modification-dependent restriction endonuclease similar to Mycobacterium MspJI and Azoarcus AspBHI

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
COG1787 COG1787
Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];
36-141 2.40e-54

Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];


:

Pssm-ID: 441393  Cd Length: 119  Bit Score: 167.74  E-value: 2.40e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  36 NQLRQSNINQINQMNGRRFEEYLSSLYQSLGYQAEVTKGSGDFGADLVLKNNGTKIVVQAKRYKNKVGIQSVQEVVGAKR 115
Cdd:COG1787     4 KRKIRSGLEELDNMDGREFEKYLAKLLRKLGYDVEVTPGSGDFGADLIATKDGERIAVQCKRYKSPVGNKAVQEVVAAKA 83

                          90       100
                  ....*....|....*....|....*.
gi 1267774385 116 YYDAAHAWVVTNNYFTEPARKLAQAN 141
Cdd:COG1787    84 YYGADKAVVVTNSYFTKSAKELAKSN 109
 
Name Accession Description Interval E-value
COG1787 COG1787
Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];
36-141 2.40e-54

Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];


Pssm-ID: 441393  Cd Length: 119  Bit Score: 167.74  E-value: 2.40e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  36 NQLRQSNINQINQMNGRRFEEYLSSLYQSLGYQAEVTKGSGDFGADLVLKNNGTKIVVQAKRYKNKVGIQSVQEVVGAKR 115
Cdd:COG1787     4 KRKIRSGLEELDNMDGREFEKYLAKLLRKLGYDVEVTPGSGDFGADLIATKDGERIAVQCKRYKSPVGNKAVQEVVAAKA 83

                          90       100
                  ....*....|....*....|....*.
gi 1267774385 116 YYDAAHAWVVTNNYFTEPARKLAQAN 141
Cdd:COG1787    84 YYGADKAVVVTNSYFTKSAKELAKSN 109
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 46-141 8.65e-35

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 118.06  E-value: 8.65e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  46 INQMNGRRFEEYLSSLYQSLGYQAEVTKGSGDFGADLVLK---NNGTKIVVQAKRYKNKVGIQSVQEVVGAKRYYDAAHA 122
Cdd:pfam04471   1 LDKMSPKEFEELVAELLRAMGYKVEVTGRSGDGGVDLIARkdpLGLERILVQAKRYKNTVGVPAVQELVGALAGYGADKG 80

                          90
                  ....*....|....*....
gi 1267774385 123 WVVTNNYFTEPARKLAQAN 141
Cdd:pfam04471  81 IFVTTSDFTPDAREFAKAL 99
MspjI-like cd22335
Modification-dependent restriction endonuclease MspjI and similar endonucleases; MspJI ...
 71-134 7.76e-04

Modification-dependent restriction endonuclease MspjI and similar endonucleases; MspJI recognizes 5-methylcytosine or 5-hydroxymethylcytosine as part of the motif CNN(G/A) and cleaves both strands at fixed distances (N(12)/N(16)) away from the modified cytosine at the 3'-side. It belongs to a superfamily of PDDEXK nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411739  Cd Length: 185  Bit Score: 38.48  E-value: 7.76e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1267774385  71 VTKGSGDFGADLVLKNN------GTKIVV--QAKRY--KNKVGIQSVQEVVGA-KRyydaahAWV---VTNNYFTEPA 134
Cdd:cd22335    57 ITKGSGDGGIDFVGRLDigsgfsSTKLVVlgQAKCEqpTSPTSGKDIARTVARlRR------GWIgvyVTTSYFSEQV 128
 
Name Accession Description Interval E-value
COG1787 COG1787
Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];
36-141 2.40e-54

Endonuclease, HJR/Mrr/RecB family [Defense mechanisms];


Pssm-ID: 441393  Cd Length: 119  Bit Score: 167.74  E-value: 2.40e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  36 NQLRQSNINQINQMNGRRFEEYLSSLYQSLGYQAEVTKGSGDFGADLVLKNNGTKIVVQAKRYKNKVGIQSVQEVVGAKR 115
Cdd:COG1787     4 KRKIRSGLEELDNMDGREFEKYLAKLLRKLGYDVEVTPGSGDFGADLIATKDGERIAVQCKRYKSPVGNKAVQEVVAAKA 83

                          90       100
                  ....*....|....*....|....*.
gi 1267774385 116 YYDAAHAWVVTNNYFTEPARKLAQAN 141
Cdd:COG1787    84 YYGADKAVVVTNSYFTKSAKELAKSN 109
Mrr_cat pfam04471
Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing ...
 46-141 8.65e-35

Restriction endonuclease; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA.


Pssm-ID: 461322  Cd Length: 114  Bit Score: 118.06  E-value: 8.65e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  46 INQMNGRRFEEYLSSLYQSLGYQAEVTKGSGDFGADLVLK---NNGTKIVVQAKRYKNKVGIQSVQEVVGAKRYYDAAHA 122
Cdd:pfam04471   1 LDKMSPKEFEELVAELLRAMGYKVEVTGRSGDGGVDLIARkdpLGLERILVQAKRYKNTVGVPAVQELVGALAGYGADKG 80

                          90
                  ....*....|....*....
gi 1267774385 123 WVVTNNYFTEPARKLAQAN 141
Cdd:pfam04471  81 IFVTTSDFTPDAREFAKAL 99
Mrr COG1715
Restriction endonuclease Mrr [Defense mechanisms];
30-141 6.64e-29

Restriction endonuclease Mrr [Defense mechanisms];


Pssm-ID: 441321  Cd Length: 157  Bit Score: 104.25  E-value: 6.64e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1267774385  30 AYNLYLNQLRQSNINQINQMNGRRFEEYLSSLYQSLGY-QAEVTKGSGDFGADLVLKNN---GTKIVVQAKRYK-NKVGI 104
Cdd:COG1715     7 AYEEIRAELAEELLERLRSLSPYEFEELVAELLRRMGYgSVEVTGRSGDGGIDGIAKEDrlgGDRIYVQAKRYKgNTVGR 86

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1267774385 105 QSVQEVVGAKRYYDAAHAWVVTNNYFTEPARKLAQAN 141
Cdd:COG1715    87 PEVREFAGALAGEGADKGIFITTSRFTKDAREEARRL 123
MspjI-like cd22335
Modification-dependent restriction endonuclease MspjI and similar endonucleases; MspJI ...
 71-134 7.76e-04

Modification-dependent restriction endonuclease MspjI and similar endonucleases; MspJI recognizes 5-methylcytosine or 5-hydroxymethylcytosine as part of the motif CNN(G/A) and cleaves both strands at fixed distances (N(12)/N(16)) away from the modified cytosine at the 3'-side. It belongs to a superfamily of PDDEXK nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411739  Cd Length: 185  Bit Score: 38.48  E-value: 7.76e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1267774385  71 VTKGSGDFGADLVLKNN------GTKIVV--QAKRY--KNKVGIQSVQEVVGA-KRyydaahAWV---VTNNYFTEPA 134
Cdd:cd22335    57 ITKGSGDGGIDFVGRLDigsgfsSTKLVVlgQAKCEqpTSPTSGKDIARTVARlRR------GWIgvyVTTSYFSEQV 128
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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