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Conserved domains on  [gi|1330003054|ref|WP_102257888|]
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MULTISPECIES: DUF3466 family protein [Vibrio]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DUF3466 super family cl26415
Protein of unknown function (DUF3466); This family of proteins are functionally ...
 281-574 1.08e-33

Protein of unknown function (DUF3466); This family of proteins are functionally uncharacterized. This protein is found in bacteria. Proteins in this family are typically between 564 to 612 amino acids in length.


The actual alignment was detected with superfamily member pfam11949:

Pssm-ID: 432213 [Multi-domain]  Cd Length: 605  Bit Score: 135.74  E-value: 1.08e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 281 VGYNTV--DGSGDLQDMNASVFIsksldlADSANTWTTKSIEGAKVNSNSSNDYKYsNSVATDINKNLFVIGNSKRNgrv 358
Cdd:pfam11949 330 VGRSNVyrYGDKDKLYLDAAYWT------KNDGGIYSYKWIPMEEVEEVDQDDQIY-NSIAYDINDNGLVVGSYRKY--- 399

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 359 pENGSAGSKMFVVtdaSVATPTATYLTGGIFFAGSSGEARAVNNFNEIVGQVDAETtreVDGSERRHRGFIYPYQATGTD 438
Cdd:pfam11949 400 -INGYLRNKFFYF---DINAGTTKIVFPNDFFSGLSSKAKDINNKGQVVGYIEVTT---DKGKPRRQAAFLYDKNTDEFS 472

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 439 PSRIDLFQNKAWFLDDLTND----GNVSGENNKFRIIDASGINDAGVISATAIKCMIDTNGDGRGNDERAYDTTSHnsyc 514
Cdd:pfam11949 473 NLNDLLTCDSAGYVKDLDGDqdgtGKTLTYNNDYRIVEANSINDAGTIVATAFVRKPKYDTDSTGNVVVEFELDGN---- 548

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 515 aGAASNAvEEVVAVKLIPIEGkGKSDIQARSTDTAKVDRKGGSLGWFALTVLGLLGFRRK 574
Cdd:pfam11949 549 -GNPVTS-YLPRAVVLKPITS-GTICTEDDPEEDENYERSGASFSWAWLFALPLLWFRRR 605
 
Name Accession Description Interval E-value
DUF3466 pfam11949
Protein of unknown function (DUF3466); This family of proteins are functionally ...
 281-574 1.08e-33

Protein of unknown function (DUF3466); This family of proteins are functionally uncharacterized. This protein is found in bacteria. Proteins in this family are typically between 564 to 612 amino acids in length.


Pssm-ID: 432213 [Multi-domain]  Cd Length: 605  Bit Score: 135.74  E-value: 1.08e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 281 VGYNTV--DGSGDLQDMNASVFIsksldlADSANTWTTKSIEGAKVNSNSSNDYKYsNSVATDINKNLFVIGNSKRNgrv 358
Cdd:pfam11949 330 VGRSNVyrYGDKDKLYLDAAYWT------KNDGGIYSYKWIPMEEVEEVDQDDQIY-NSIAYDINDNGLVVGSYRKY--- 399

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 359 pENGSAGSKMFVVtdaSVATPTATYLTGGIFFAGSSGEARAVNNFNEIVGQVDAETtreVDGSERRHRGFIYPYQATGTD 438
Cdd:pfam11949 400 -INGYLRNKFFYF---DINAGTTKIVFPNDFFSGLSSKAKDINNKGQVVGYIEVTT---DKGKPRRQAAFLYDKNTDEFS 472

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 439 PSRIDLFQNKAWFLDDLTND----GNVSGENNKFRIIDASGINDAGVISATAIKCMIDTNGDGRGNDERAYDTTSHnsyc 514
Cdd:pfam11949 473 NLNDLLTCDSAGYVKDLDGDqdgtGKTLTYNNDYRIVEANSINDAGTIVATAFVRKPKYDTDSTGNVVVEFELDGN---- 548

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 515 aGAASNAvEEVVAVKLIPIEGkGKSDIQARSTDTAKVDRKGGSLGWFALTVLGLLGFRRK 574
Cdd:pfam11949 549 -GNPVTS-YLPRAVVLKPITS-GTICTEDDPEEDENYERSGASFSWAWLFALPLLWFRRR 605
GlyGly_CTERM TIGR03501
GlyGly-CTERM domain; This homology domain, GlyGly-CTERM, shares a species distribution with ...
 555-575 2.57e-05

GlyGly-CTERM domain; This homology domain, GlyGly-CTERM, shares a species distribution with rhombosortase (TIGR03902), a subfamily of rhomboid-like intramembrane serine proteases. It is probably a recognition sequence for protein sorting and then cleavage by rhombosortase. Shewanella species have the largest number of target proteins per genome, up to thirteen. The domain occurs at the extreme carboxyl-terminus of a diverse set of proteins, most of which are enzymes with conventional signal sequences and with hydrolytic activities: nucleases, proteases, agarases, etc. The agarase AgaA from Vibro sp. strain JT0107 is secreted into the medium, while the same protein heterologously expressed in E. coli is retained in the cell fraction. This suggests cleavage and release in species with this domain. Both this suggestion, and the chemical structure of the domain (motif, hydrophobic predicted transmembrane helix, cluster of basic residues) closely parallels that of the LPXTG/sortase system and the PEP-CTERM/exosortase(EpsH) system. For this reason, the putative processing enzyme is designated rhombosortase.


Pssm-ID: 274611  Cd Length: 22  Bit Score: 41.06  E-value: 2.57e-05
                          10        20
                  ....*....|....*....|.
gi 1330003054 555 GGSLGWFALTVLGLLGFRRKF 575
Cdd:TIGR03501   2 GGSLGWLSLLLLLLLGLRRRK 22
 
Name Accession Description Interval E-value
DUF3466 pfam11949
Protein of unknown function (DUF3466); This family of proteins are functionally ...
 281-574 1.08e-33

Protein of unknown function (DUF3466); This family of proteins are functionally uncharacterized. This protein is found in bacteria. Proteins in this family are typically between 564 to 612 amino acids in length.


Pssm-ID: 432213 [Multi-domain]  Cd Length: 605  Bit Score: 135.74  E-value: 1.08e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 281 VGYNTV--DGSGDLQDMNASVFIsksldlADSANTWTTKSIEGAKVNSNSSNDYKYsNSVATDINKNLFVIGNSKRNgrv 358
Cdd:pfam11949 330 VGRSNVyrYGDKDKLYLDAAYWT------KNDGGIYSYKWIPMEEVEEVDQDDQIY-NSIAYDINDNGLVVGSYRKY--- 399

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 359 pENGSAGSKMFVVtdaSVATPTATYLTGGIFFAGSSGEARAVNNFNEIVGQVDAETtreVDGSERRHRGFIYPYQATGTD 438
Cdd:pfam11949 400 -INGYLRNKFFYF---DINAGTTKIVFPNDFFSGLSSKAKDINNKGQVVGYIEVTT---DKGKPRRQAAFLYDKNTDEFS 472

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 439 PSRIDLFQNKAWFLDDLTND----GNVSGENNKFRIIDASGINDAGVISATAIKCMIDTNGDGRGNDERAYDTTSHnsyc 514
Cdd:pfam11949 473 NLNDLLTCDSAGYVKDLDGDqdgtGKTLTYNNDYRIVEANSINDAGTIVATAFVRKPKYDTDSTGNVVVEFELDGN---- 548

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1330003054 515 aGAASNAvEEVVAVKLIPIEGkGKSDIQARSTDTAKVDRKGGSLGWFALTVLGLLGFRRK 574
Cdd:pfam11949 549 -GNPVTS-YLPRAVVLKPITS-GTICTEDDPEEDENYERSGASFSWAWLFALPLLWFRRR 605
GlyGly_CTERM TIGR03501
GlyGly-CTERM domain; This homology domain, GlyGly-CTERM, shares a species distribution with ...
 555-575 2.57e-05

GlyGly-CTERM domain; This homology domain, GlyGly-CTERM, shares a species distribution with rhombosortase (TIGR03902), a subfamily of rhomboid-like intramembrane serine proteases. It is probably a recognition sequence for protein sorting and then cleavage by rhombosortase. Shewanella species have the largest number of target proteins per genome, up to thirteen. The domain occurs at the extreme carboxyl-terminus of a diverse set of proteins, most of which are enzymes with conventional signal sequences and with hydrolytic activities: nucleases, proteases, agarases, etc. The agarase AgaA from Vibro sp. strain JT0107 is secreted into the medium, while the same protein heterologously expressed in E. coli is retained in the cell fraction. This suggests cleavage and release in species with this domain. Both this suggestion, and the chemical structure of the domain (motif, hydrophobic predicted transmembrane helix, cluster of basic residues) closely parallels that of the LPXTG/sortase system and the PEP-CTERM/exosortase(EpsH) system. For this reason, the putative processing enzyme is designated rhombosortase.


Pssm-ID: 274611  Cd Length: 22  Bit Score: 41.06  E-value: 2.57e-05
                          10        20
                  ....*....|....*....|.
gi 1330003054 555 GGSLGWFALTVLGLLGFRRKF 575
Cdd:TIGR03501   2 GGSLGWLSLLLLLLLGLRRRK 22
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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