View
Concise Results
Standard Results
Full Results
glycyl radical protein [Faecalibacillus faecis]
Protein Classification
glycyl radical protein ( domain architecture ID 10109344 )
glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase
List of domain hits
Name
Accession
Description
Interval
E-value
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
11-800
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1142.40
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 11 T P R MNHFREEV L DK KP Y I CAK RA L L A TE A YKE HLNE P P I MK RA YM LK N ILE KMP IYI EE D T LIVGN QASSN R DA P I FPE Y 90
Cdd:cd01677 1 T E R IKRLKAKI L TA KP S I SIE RA R L Y TE S YKE TEGK P V I IR RA KA LK H ILE NAT IYI QD D E LIVGN RGGKP R AV P V FPE L 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 91 TMEF V MN ELD LFE KR D GD V F Y I T E ET KE E L RS I A P F W ENNN LR SKGGALL P D E VSVY M ET G F F GMEGKLN SG DA H L AVDY 170
Cdd:cd01677 81 SVHW V ED ELD DLP KR P GD P F V I S E DK KE Y L EE I F P Y W KGKT LR DRCFKYF P E E TLIA M AA G V F TEFMYFF SG PG H V AVDY 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 171 QQ VL Q KGL K G YEKRV K DLK E S LDL CI PE N IDK YQ FY K A VL IV I EAV K T F A N RY S ELA L ELA - RSAEG KRK E EL E EIA RV C 249
Cdd:cd01677 161 PK VL E KGL D G LIEEA K EAI E A LDL TG PE D IDK IY FY Q A MI IV C EAV I T Y A K RY A ELA K ELA a KETDP KRK A EL L EIA EI C 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 250 R K VP YEKAE TF Y EA I QS T WFI Q LILQIESNGHS L S Y GRFDQY I YP Y YK H D KDLNNI T E E Q A V ELL DN LWIK TLT INKVRS 329
Cdd:cd01677 241 R R VP AHPPR TF W EA L QS F WFI H LILQIESNGHS I S P GRFDQY L YP F YK Q D IEEGRL T R E G A I ELL EC LWIK INE INKVRS 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 330 Q A HTFSS AG SPMY QN V TIGGQT P D KK DATNELSYL V L KSVAQT RLPQP N LTVRYH KNM P KA FL DE A I EV MK LG T G M PAF N 409
Cdd:cd01677 321 G A SAKYF AG YNTF QN L TIGGQT E D GS DATNELSYL I L EATRRV RLPQP S LTVRYH AKS P DK FL KK A A EV IR LG L G Y PAF F 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 410 NDE II IP SFIE KGV KE EDA YN Y SA IGCVET AV PG k WG YR C TG MS Y M N FPRI L LMAM N D G V D MT SGK RFFEGS G YFK D MTS 489
Cdd:cd01677 401 NDE VV IP ALLR KGV SL EDA RD Y GL IGCVET GA PG - RK YR W TG TG Y I N LAKV L EITL N N G K D PR SGK QVGPET G DAT D FKT 479
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 490 YD EL FK A IE K GM R YLTRM SV IVE N AI D L A LERDV P DVLC SAL TE DCI GR GK T I KE GGA V Y D F ISGLQ VGIA NMA DSL V AI 569
Cdd:cd01677 480 FE EL WE A FK K QL R HFIKL SV RAN N IS D I A HAEVA P APFL SAL VD DCI EK GK D I NA GGA R Y N F GGIQG VGIA TLG DSL A AI 559
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 570 KKLVFEEKK I T P EEL WN A ILDDY ts K E SQTIQEM L I N DV PKYGND I D EV D QLVVD VY NV Y ID E MK KY P N trygrg P I GG I 649
Cdd:cd01677 560 KKLVFEEKK L T M EEL LE A LKANF -- A E GYEERRR L L N NA PKYGND D D YA D NIARR VY EW Y CK E VE KY Q N ------ P R GG K 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 650 R Y A GT S S I SANV GQ G YG T M ATPDGR K A H TPL AE G C SP AHAM DKKGPTAV F KSVSKL PTHE I T GG V LLNQK VT P QL L SK EE 729
Cdd:cd01677 632 F Y P GT Y S V SANV PF G SV T G ATPDGR L A G TPL SD G V SP SQGT DKKGPTAV I KSVSKL DHFN I S GG T LLNQK FS P ST L EG EE 711
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1473344021 730 NKE K IEM LI K T F F n R L K G Y H V Q Y NVVS K ETL L DAQ A HPEK HK DLIVRVAGYSA F F NV LSK AT QD D II G RTE 800
Cdd:cd01677 712 GLK K LAA LI R T Y F - D L G G H H I Q F NVVS A ETL R DAQ K HPEK YR DLIVRVAGYSA Y F VE LSK EV QD E II A RTE 781
Name
Accession
Description
Interval
E-value
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
11-800
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1142.40
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 11 T P R MNHFREEV L DK KP Y I CAK RA L L A TE A YKE HLNE P P I MK RA YM LK N ILE KMP IYI EE D T LIVGN QASSN R DA P I FPE Y 90
Cdd:cd01677 1 T E R IKRLKAKI L TA KP S I SIE RA R L Y TE S YKE TEGK P V I IR RA KA LK H ILE NAT IYI QD D E LIVGN RGGKP R AV P V FPE L 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 91 TMEF V MN ELD LFE KR D GD V F Y I T E ET KE E L RS I A P F W ENNN LR SKGGALL P D E VSVY M ET G F F GMEGKLN SG DA H L AVDY 170
Cdd:cd01677 81 SVHW V ED ELD DLP KR P GD P F V I S E DK KE Y L EE I F P Y W KGKT LR DRCFKYF P E E TLIA M AA G V F TEFMYFF SG PG H V AVDY 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 171 QQ VL Q KGL K G YEKRV K DLK E S LDL CI PE N IDK YQ FY K A VL IV I EAV K T F A N RY S ELA L ELA - RSAEG KRK E EL E EIA RV C 249
Cdd:cd01677 161 PK VL E KGL D G LIEEA K EAI E A LDL TG PE D IDK IY FY Q A MI IV C EAV I T Y A K RY A ELA K ELA a KETDP KRK A EL L EIA EI C 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 250 R K VP YEKAE TF Y EA I QS T WFI Q LILQIESNGHS L S Y GRFDQY I YP Y YK H D KDLNNI T E E Q A V ELL DN LWIK TLT INKVRS 329
Cdd:cd01677 241 R R VP AHPPR TF W EA L QS F WFI H LILQIESNGHS I S P GRFDQY L YP F YK Q D IEEGRL T R E G A I ELL EC LWIK INE INKVRS 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 330 Q A HTFSS AG SPMY QN V TIGGQT P D KK DATNELSYL V L KSVAQT RLPQP N LTVRYH KNM P KA FL DE A I EV MK LG T G M PAF N 409
Cdd:cd01677 321 G A SAKYF AG YNTF QN L TIGGQT E D GS DATNELSYL I L EATRRV RLPQP S LTVRYH AKS P DK FL KK A A EV IR LG L G Y PAF F 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 410 NDE II IP SFIE KGV KE EDA YN Y SA IGCVET AV PG k WG YR C TG MS Y M N FPRI L LMAM N D G V D MT SGK RFFEGS G YFK D MTS 489
Cdd:cd01677 401 NDE VV IP ALLR KGV SL EDA RD Y GL IGCVET GA PG - RK YR W TG TG Y I N LAKV L EITL N N G K D PR SGK QVGPET G DAT D FKT 479
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 490 YD EL FK A IE K GM R YLTRM SV IVE N AI D L A LERDV P DVLC SAL TE DCI GR GK T I KE GGA V Y D F ISGLQ VGIA NMA DSL V AI 569
Cdd:cd01677 480 FE EL WE A FK K QL R HFIKL SV RAN N IS D I A HAEVA P APFL SAL VD DCI EK GK D I NA GGA R Y N F GGIQG VGIA TLG DSL A AI 559
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 570 KKLVFEEKK I T P EEL WN A ILDDY ts K E SQTIQEM L I N DV PKYGND I D EV D QLVVD VY NV Y ID E MK KY P N trygrg P I GG I 649
Cdd:cd01677 560 KKLVFEEKK L T M EEL LE A LKANF -- A E GYEERRR L L N NA PKYGND D D YA D NIARR VY EW Y CK E VE KY Q N ------ P R GG K 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 650 R Y A GT S S I SANV GQ G YG T M ATPDGR K A H TPL AE G C SP AHAM DKKGPTAV F KSVSKL PTHE I T GG V LLNQK VT P QL L SK EE 729
Cdd:cd01677 632 F Y P GT Y S V SANV PF G SV T G ATPDGR L A G TPL SD G V SP SQGT DKKGPTAV I KSVSKL DHFN I S GG T LLNQK FS P ST L EG EE 711
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1473344021 730 NKE K IEM LI K T F F n R L K G Y H V Q Y NVVS K ETL L DAQ A HPEK HK DLIVRVAGYSA F F NV LSK AT QD D II G RTE 800
Cdd:cd01677 712 GLK K LAA LI R T Y F - D L G G H H I Q F NVVS A ETL R DAQ K HPEK YR DLIVRVAGYSA Y F VE LSK EV QD E II A RTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
4-803
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1094.04
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 4 SEHFGEL T P R MNHF RE EV L DK KP Y I CAK RA L L A TE A YKE HLNE P P I MK RA YMLKN ILE KM PIYI EE D T LIVG N Q ASSN R D 83
Cdd:COG1882 1 ESFLAGP T E R TKRL RE KL L EA KP L I DIE RA R L F TE S YKE TEGL P V I IR RA KAFSH ILE HK PIYI KD D E LIVG L Q TDKP R K 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 84 A PIFPE YTMEF V MN ELD LFEK R DG D V F Y I TE E T KE EL R S IAP F W ENNNLRSKGGALL P D E VSVYMET G FFG m EGKLNS G D 163
Cdd:COG1882 81 R PIFPE GGIRW V ED ELD ALPT R PQ D G F E I SP E D KE IF R E IAP Y W KGKTHNDGVFDAY P E E IRKARKA G IIT - GLPDAY G R 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 164 A H LAV DY QQ VL QK GL K G YEKRV K DLKES LDL CI PE N I D K YQ FYKA VL IV I EAV KTF A N RY S ELA L ELA RS - AEG KRK E EL 242
Cdd:COG1882 160 G H IIG DY RR VL LY GL D G LIEEA K EKLAE LDL TD PE D I E K ID FYKA MI IV C EAV IRL A E RY A ELA R ELA EK e TDP KRK A EL 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 243 E EIA RV C RK VP YEK A E TF Y EA I Q ST WF IQ L ILQI E S NG HSL S Y GRFDQY I YPYY KH D KDLNNI TEE Q A V ELLD NL WIK TL 322
Cdd:COG1882 240 L EIA EI C GF VP ANP A R TF W EA V Q WV WF VY L AAIK E Q NG AAM S L GRFDQY L YPYY ER D LEEGRL TEE E A Q ELLD CF WIK LR 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 323 TINKV R SQAHTFSS AG S P MYQNV TIGG Q TPD KK DA T NELSYL V L KSVAQTR LP Q PNLTVR YHKNM P KA FL DE A I EV MKL G 402
Cdd:COG1882 320 EVRFL R TPEYAELF AG Y P TWVTL TIGG M TPD GR DA V NELSYL I L ETLRNLP LP E PNLTVR WSEKL P EG FL KK A A EV ISI G 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 403 TG M P AFN ND EII IP SFIE KGV KE EDA YN Y SAI GCVE TA VPGK W g YRCT G MSYM N FPRI L LM A M N D GVD MTS GK RFFEGS G 482
Cdd:COG1882 400 TG S P QYE ND DLM IP MLLN KGV TL EDA RD Y GIA GCVE PM VPGK Q - MQFF G AGRI N LAKA L EY A L N N GVD EKT GK QVGPET G 478
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 483 YFK D MTS YDE LFK A IE K GMR YL TRMS V IVE N A I DLALERDV P DVLC SAL TE DCI G RGK TIK EGGA V Y D F ISGLQV G IANM 562
Cdd:COG1882 479 DPT D FLT YDE VME A FK K QLD YL ADLY V NAL N I I HYMHDKYA P EPFL SAL HD DCI E RGK DLN EGGA R Y N F GAIGIA G LSVV 558
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 563 ADSL V AIKKLVF EE KK I T PE EL WN A ILDDYTSK E S qt IQEM L I N d V PKYGND I D E VD QLV V DVYNVYI DE MK KY PNT R yg 642
Cdd:COG1882 559 ADSL S AIKKLVF DK KK V T MD EL LE A LAANFEGY E E -- LRQL L L N - A PKYGND D D Y VD EIA V ELVETFM DE IR KY KTY R -- 633
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 643 rgpi GG IRYAGTSS I SA NV GQ G YG T M ATPDGRKA HT PLA E G C SP A H AM DK K GPTAV F KSV S KLP THEI T G G V LLNQK VT P 722
Cdd:COG1882 634 ---- GG TYTLSILT I TS NV PY G KK T G ATPDGRKA GE PLA D G A SP M H GR DK N GPTAV L KSV A KLP YEKA T D G I LLNQK FS P 709
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 723 QL L SK EE NK E KIEM L IK T F F N r L K G Y H V Q Y NVV SK ETLLDAQ A HPEK HK DL I VRVAGYSA F F NV LSK AT QDDII G RTE QT 802
Cdd:COG1882 710 SA L GG EE GI E NLVS L LR T Y F D - L G G H H I Q F NVV DR ETLLDAQ K HPEK YP DL T VRVAGYSA Y F VE LSK EQ QDDII A RTE HE 788
.
gi 1473344021 803 L 803
Cdd:COG1882 789 F 789
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
10-803
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 868.07
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 LTP R MNHFREEVLDKKPYICAK RA L L A TE AY K EHLN EP P I MK RA YM L KNI LE KM PI Y I EEDT LIVGN QASSN R D A P IFPE 89
Cdd:TIGR01774 2 CMD R IEKLKKALSKPVARLSVE RA R L Y TE SM K QTEG EP M I IR RA KA L AHV LE NI PI Q I LDSE LIVGN MLPNP R A A I IFPE 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 90 YTMEFVMN ELD LFEK R DGDV F YIT EE T K EE LR S - IAP F W ENNNLRSKGGA L LP DE VSVYME T GF F GME G k LNS G DA H L AV 168
Cdd:TIGR01774 82 GVGLWIIK ELD SLPN R PTNR F AVS EE D K RV LR E e IAP Y W QRKTIEDFAFG L MT DE MKILYT T SI F VLT G - EIA G IS H V AV 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 169 D Y QQV L QK GL KGYEKRVKDLKES L DLCIPENID KY Q FY K A VL IV I EAV KTFAN RY SE LA L E L A R S AE G - K R K EEL EE IA R 247
Cdd:TIGR01774 161 N Y PYL L RR GL RWLLEESERRIRA L EESGVYEGE KY S FY Q A AK IV L EAV INHIL RY AK LA E E M A A S ET G e S R R EEL LK IA E 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 248 V CRKV PY EK AE TF YE A I Q ST W FI Q L ILQ I ESN GH S L S Y GR F DQY I YP Y YK H D KDLNN I TE E Q A V E L L DN LWIKT LT I NKV 327
Cdd:TIGR01774 241 I CRKV AA EK PQ TF WQ A V Q LV W LV Q S ILQ Q ESN EQ S I S M GR I DQY L YP F YK K D IGEGR I DR E L A F E I L AS LWIKT NE I VPA 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 328 RS QAHTFSS AG S P MY Q N VTIGG QTPDKK DA T NELSYL V L KSVAQT RL P QPNL T VR YHKNM P KA FL DEAI E VMKL G T G M PA 407
Cdd:TIGR01774 321 RS SSLEQYF AG Q P TN Q A VTIGG CDIYGN DA V NELSYL M L EVTDRL RL R QPNL H VR INAGS P ES FL KRLA E AIRS G C G N PA 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 408 FN NDE III P S F IEK GV KEE DA YN Y SAI GCVE T A VP G KW g Y RCTGMSYM N FPRILLM A M N D GVD MTS G KR F FEGS gyf KDM 487
Cdd:TIGR01774 401 LF NDE AVV P A F KNA GV DDR DA LD Y TTD GCVE I A PF G NS - Y TSSDAALI N VAKVMEY A L N E GVD LQF G YE F GAKT --- EKP 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 488 TSYDE L FKAIEKGMRYLTRMS V IVE N AI D L A LERDV P DV L C S ALT EDC IGR G KT I KE GGA V Y D F ISGLQ VGIAN MA DSLV 567
Cdd:TIGR01774 477 KFLED L LEKLREQISHYVKLV V EGS N VL D Y A NAEVK P TP L L S LCV EDC FEK G VD I SR GGA R Y N F TGIQA VGIAN LG DSLV 556
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 568 AIK KL VFE EKKITPEE L WN A ILDDYTSKESQTIQEM LIN DV PKYGND I D E VD QLVVD V YNV Y ID E MKK Y P N T R ygrgpi G 647
Cdd:TIGR01774 557 AIK GA VFE QGAYSMDD L VE A LRKNFVGLTHEEKRHR LIN QS PKYGND D D A VD KYTAM V LEW Y CE E VNR Y R N P R ------ G 630
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 648 G IRY AG TSSI SANV GQ G YG T M ATPDGRKA HT PLA E G C SP AHA MD KK GPTAV FK SVSKL PTHEITG G V LLN Q K VT P QL L SK 727
Cdd:TIGR01774 631 G KFA AG CYPM SANV PF G FF T S ATPDGRKA GE PLA P G V SP STG MD RE GPTAV IN SVSKL SYENLPN G A LLN I K LS P AT L EE 710
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1473344021 728 E EN K E K IEMLIKTF F NR LK GY H V Q Y N V V SKE TL LD AQ AH PEK HKD L I VRVAGYSAFF NV LS KAT QDDII G RT EQT L 803
Cdd:TIGR01774 711 E GD K Q K LIEALRKS F ME LK VM H I Q F N I V KED TL RK AQ QE PEK YRW L V VRVAGYSAFF VE LS RPV QDDII R RT SHR L 786
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
10-664
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 748.69
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 LTP R MNHFR E EVL -- DKK P YICAK RA L L A TE A YKE HLNEP P I - MK RA YM LK N IL EKM P I YI EE D T LIVG N Q ASSN R DAP I 86
Cdd:pfam02901 1 MWE R IDVLK E NYT ly TGD P SLSWE RA R L L TE S YKE TEGVL P V d IR RA KA LK K IL SHL P G YI RD D E LIVG L Q TDKP R KRA I 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 87 F PE YTMEF V MN ELD LFEK R DG D V F Y I T EE T K EEL R S I A P F W ENNNLRSKGGALLPD E VSVYM E T G F F g MEGKLNS G DA H L 166
Cdd:pfam02901 81 Y PE GGIRW V ED ELD YLNT R PQ D G F E I S EE D K KIF R E I F P Y W KGKTHNEGVFDAYTP E MKAAR E S G I F - TGLPDAY G RG H I 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 167 AV DY QQ VL QK GL K G YEKRVKDLKES LD l CI PE N I D K YQ FYKA VL I VIE AV KTF A N RY SE LA L ELA RS - AEG KRK E EL E EI 245
Cdd:pfam02901 160 IG DY RR VL LY GL D G LIEEKEEKLAK LD - TD PE D I E K IE FYKA MI I SCD AV IEY A E RY AR LA E ELA EQ e TDP KRK A EL L EI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 246 A RV C RK VP YEK AETF Y EAIQ ST WF IQ L ILQI E S NG HSL S Y GR F DQY I YPYY KH D KDLNNI TEE Q A V EL L D NL WIK TLTIN 325
Cdd:pfam02901 239 A EI C GR VP ARP AETF Q EAIQ WF WF VY L AAVK E Q NG AAM S L GR L DQY L YPYY ER D LEEGRL TEE E A Q EL I D CF WIK LREVR 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 326 KV R SQAHTFSS AG SPMY QN V TIGGQ TP D KK DA T N E LSYL V L KSVAQTR LP Q PNLTVR YH K NM P KA FL DE A I EV MKL GTG M 405
Cdd:pfam02901 319 FL R TPEYNKLF AG YDPF QN L TIGGQ GR D GR DA V N K LSYL I L EALDNLP LP E PNLTVR WS K KL P EE FL KK A A EV SRK GTG S 398
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 406 P AFN ND EII IP SFIEK GV KE EDA YN Y SAI GCVE TAV PGK - WGYRC tgm SYM N FPRI L LM A M N D G V D MTS GK RFFEGS G YF 484
Cdd:pfam02901 399 P QYE ND DVM IP ALLNR GV SL EDA RD Y GIA GCVE PMK PGK e MQFFG --- ARI N LAKA L EY A L N G G R D ELT GK QVGPKT G PV 475
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 485 KDMT S YD E LFK A IE K GMR YL TRMS V IVE N A I DLALERDV P DVLC SAL TE DCI G RGK TIKE GGA V Y D F ISGLQV G I AN M AD 564
Cdd:pfam02901 476 TEFL S FE E VME A FK K QLD YL ADLY V NAL N I I HYMHDKYA P EPFL SAL HD DCI E RGK DVGI GGA R Y N F SGPQGA G L AN V AD 555
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 565 SL V AIKKLVF EE K KI T PE EL WN A ILD D YTSK E S qt IQEM L I ND V PKYGND I D E VD QLV V D V YNVYI DE MK KY P N T R ygrg 644
Cdd:pfam02901 556 SL S AIKKLVF DD K VY T LR EL ED A LAA D FEGE E E -- LRQD L L ND A PKYGND D D R VD DIA V E V VETFM DE VR KY K N Y R ---- 629
650 660
....*....|....*....|
gi 1473344021 645 pi GG IRYAGTSS I SA NV GQ G 664
Cdd:pfam02901 630 -- GG KFTPSLLT I TS NV PY G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
10-803
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 569.07
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 L T P R MNHFREEVLDKKPY I CAK RALL A T EAYKEHLN EP P I MK RA YMLKN ILE KMP I Y I EEDT LI V GN QASSN R DAPIF PE 89
Cdd:PRK09983 1 M T N R ISRLKTALFANTRE I SLE RALL Y T ASHRQTEG EP V I LR RA KATAY ILE HVE I S I RDEE LI A GN RTVKP R AGIMS PE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 90 YTMEFVMN ELD L F EK R DG D V F Y I T EE T K EEL R - SIA P F WE NNNLRSKGGALLP DEV SVYME T GF F GME g KLNS G DA H LAV 168
Cdd:PRK09983 81 MDPYWLLK ELD Q F PT R PQ D R F A I S EE D K RIY R e ELF P Y WE KRSMKDFINGQMT DEV KAATS T QI F SIN - QTDK G QG H III 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 169 DY QQV L QK GL KGYEKRVKDLKESL dlci PEN idky Q FY K A V L IVI EA VKTFAN RY S ELA LEL A - RSAEGK R K EEL EE IA R 247
Cdd:PRK09983 160 DY PRL L NH GL GELVAQMQQHCQQQ ---- PEN ---- H FY Q A A L LLL EA SQKHIL RY A ELA ETM A a NCTDAQ R R EEL LT IA E 231
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 248 VC R KVPYE K AE TF YE A I Q ST W FIQL ILQ I ESN GH SLS Y GRFDQY IY P Y Y K hd KD L N - NITEEQAV ELL DN LW I K TLT I NK 326
Cdd:PRK09983 232 IS R HNAQH K PQ TF WQ A C Q LF W YMNI ILQ Y ESN AS SLS L GRFDQY ML P F Y Q -- AS L T q GEDPAFLK ELL ES LW V K CND I VL 309
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 327 V RS QAHTFSS AG S P MYQNVTI GG Q T PDKKD A T N E LS Y L V L KSVAQTR LPQPNL T VR YHKNMPKA FL DEAI E VMK LGTG M P 406
Cdd:PRK09983 310 L RS TSSARYF AG F P TGYTALL GG L T ENGRS A V N V LS F L C L DAYQSVQ LPQPNL G VR TNALIDTP FL MKTA E TIR LGTG I P 389
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 407 AFN NDE III P S F IEK GV KE EDA YN YS AI GCVE TAV PG K w G Y RCTGMSYM N FPRILLMAMNDG vdmtsgkrff EG S gyfk D 486
Cdd:PRK09983 390 QIF NDE VVV P A F LNR GV SL EDA RD YS VV GCVE LSI PG R - T Y GLHDIAMF N LLKVMEICLHEN ---------- EG N ---- A 454
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 487 MTS Y DE L FKA I EKGMRYLTRMS V IVE N AI D LA l E RD - V P DV L C S ALTE DC IGR G KT I KE GGA V Y D F i SG L Q - V GIAN MA D 564
Cdd:PRK09983 455 ALT Y EG L LEQ I RAKISHYITLM V EGS N IC D IG - H RD w A P VP L L S SFIS DC LEK G RD I TD GGA R Y N F - SG V Q g I GIAN LS D 532
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 565 SL V A I K KL VF EEKKITPE EL WNAILDDYTSK E SQTIQEM LIN DVP KYGNDIDEVD QLVVDVYNV Y ID E MK KY P N T R ygrg 644
Cdd:PRK09983 533 SL H A L K GM VF DQQRLSFD EL LSVLKANFATP E GEKVRAR LIN RFE KYGNDIDEVD NISAELLRH Y CK E VE KY Q N P R ---- 608
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 645 pi GG IRYA G TSSI SA N V GQ G YGTM ATPDGR K A HTP LA E - G C SP AHAM D KK GPTAV F KSVSKL PTHEITG G V LLN Q K V TP Q 723
Cdd:PRK09983 609 -- GG YFTP G SYTV SA H V PL G SVVG ATPDGR F A GEQ LA D g G L SP MLGQ D AQ GPTAV L KSVSKL DNTLLSN G T LLN V K F TP A 686
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 724 L L SK E ENKE K IEMLIKT f F NR LK GY H V Q Y NVV SKE TL LD AQ AH P EKHKD L I VRVAGYSAFF NV LSK AT QDDII G RT EQT L 803
Cdd:PRK09983 687 T L EG E AGLR K LADFLRA - F TQ LK LQ H I Q F NVV NAD TL RE AQ QR P QDYAG L V VRVAGYSAFF VE LSK EI QDDII R RT AHQ L 765
Name
Accession
Description
Interval
E-value
PFL2_DhaB_BssA
cd01677 Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ...
11-800
0e+00
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain]
Cd Length: 781
Bit Score: 1142.40
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 11 T P R MNHFREEV L DK KP Y I CAK RA L L A TE A YKE HLNE P P I MK RA YM LK N ILE KMP IYI EE D T LIVGN QASSN R DA P I FPE Y 90
Cdd:cd01677 1 T E R IKRLKAKI L TA KP S I SIE RA R L Y TE S YKE TEGK P V I IR RA KA LK H ILE NAT IYI QD D E LIVGN RGGKP R AV P V FPE L 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 91 TMEF V MN ELD LFE KR D GD V F Y I T E ET KE E L RS I A P F W ENNN LR SKGGALL P D E VSVY M ET G F F GMEGKLN SG DA H L AVDY 170
Cdd:cd01677 81 SVHW V ED ELD DLP KR P GD P F V I S E DK KE Y L EE I F P Y W KGKT LR DRCFKYF P E E TLIA M AA G V F TEFMYFF SG PG H V AVDY 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 171 QQ VL Q KGL K G YEKRV K DLK E S LDL CI PE N IDK YQ FY K A VL IV I EAV K T F A N RY S ELA L ELA - RSAEG KRK E EL E EIA RV C 249
Cdd:cd01677 161 PK VL E KGL D G LIEEA K EAI E A LDL TG PE D IDK IY FY Q A MI IV C EAV I T Y A K RY A ELA K ELA a KETDP KRK A EL L EIA EI C 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 250 R K VP YEKAE TF Y EA I QS T WFI Q LILQIESNGHS L S Y GRFDQY I YP Y YK H D KDLNNI T E E Q A V ELL DN LWIK TLT INKVRS 329
Cdd:cd01677 241 R R VP AHPPR TF W EA L QS F WFI H LILQIESNGHS I S P GRFDQY L YP F YK Q D IEEGRL T R E G A I ELL EC LWIK INE INKVRS 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 330 Q A HTFSS AG SPMY QN V TIGGQT P D KK DATNELSYL V L KSVAQT RLPQP N LTVRYH KNM P KA FL DE A I EV MK LG T G M PAF N 409
Cdd:cd01677 321 G A SAKYF AG YNTF QN L TIGGQT E D GS DATNELSYL I L EATRRV RLPQP S LTVRYH AKS P DK FL KK A A EV IR LG L G Y PAF F 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 410 NDE II IP SFIE KGV KE EDA YN Y SA IGCVET AV PG k WG YR C TG MS Y M N FPRI L LMAM N D G V D MT SGK RFFEGS G YFK D MTS 489
Cdd:cd01677 401 NDE VV IP ALLR KGV SL EDA RD Y GL IGCVET GA PG - RK YR W TG TG Y I N LAKV L EITL N N G K D PR SGK QVGPET G DAT D FKT 479
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 490 YD EL FK A IE K GM R YLTRM SV IVE N AI D L A LERDV P DVLC SAL TE DCI GR GK T I KE GGA V Y D F ISGLQ VGIA NMA DSL V AI 569
Cdd:cd01677 480 FE EL WE A FK K QL R HFIKL SV RAN N IS D I A HAEVA P APFL SAL VD DCI EK GK D I NA GGA R Y N F GGIQG VGIA TLG DSL A AI 559
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 570 KKLVFEEKK I T P EEL WN A ILDDY ts K E SQTIQEM L I N DV PKYGND I D EV D QLVVD VY NV Y ID E MK KY P N trygrg P I GG I 649
Cdd:cd01677 560 KKLVFEEKK L T M EEL LE A LKANF -- A E GYEERRR L L N NA PKYGND D D YA D NIARR VY EW Y CK E VE KY Q N ------ P R GG K 631
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 650 R Y A GT S S I SANV GQ G YG T M ATPDGR K A H TPL AE G C SP AHAM DKKGPTAV F KSVSKL PTHE I T GG V LLNQK VT P QL L SK EE 729
Cdd:cd01677 632 F Y P GT Y S V SANV PF G SV T G ATPDGR L A G TPL SD G V SP SQGT DKKGPTAV I KSVSKL DHFN I S GG T LLNQK FS P ST L EG EE 711
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1473344021 730 NKE K IEM LI K T F F n R L K G Y H V Q Y NVVS K ETL L DAQ A HPEK HK DLIVRVAGYSA F F NV LSK AT QD D II G RTE 800
Cdd:cd01677 712 GLK K LAA LI R T Y F - D L G G H H I Q F NVVS A ETL R DAQ K HPEK YR DLIVRVAGYSA Y F VE LSK EV QD E II A RTE 781
PflD
COG1882 Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ...
4-803
0e+00
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain]
Cd Length: 789
Bit Score: 1094.04
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 4 SEHFGEL T P R MNHF RE EV L DK KP Y I CAK RA L L A TE A YKE HLNE P P I MK RA YMLKN ILE KM PIYI EE D T LIVG N Q ASSN R D 83
Cdd:COG1882 1 ESFLAGP T E R TKRL RE KL L EA KP L I DIE RA R L F TE S YKE TEGL P V I IR RA KAFSH ILE HK PIYI KD D E LIVG L Q TDKP R K 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 84 A PIFPE YTMEF V MN ELD LFEK R DG D V F Y I TE E T KE EL R S IAP F W ENNNLRSKGGALL P D E VSVYMET G FFG m EGKLNS G D 163
Cdd:COG1882 81 R PIFPE GGIRW V ED ELD ALPT R PQ D G F E I SP E D KE IF R E IAP Y W KGKTHNDGVFDAY P E E IRKARKA G IIT - GLPDAY G R 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 164 A H LAV DY QQ VL QK GL K G YEKRV K DLKES LDL CI PE N I D K YQ FYKA VL IV I EAV KTF A N RY S ELA L ELA RS - AEG KRK E EL 242
Cdd:COG1882 160 G H IIG DY RR VL LY GL D G LIEEA K EKLAE LDL TD PE D I E K ID FYKA MI IV C EAV IRL A E RY A ELA R ELA EK e TDP KRK A EL 239
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 243 E EIA RV C RK VP YEK A E TF Y EA I Q ST WF IQ L ILQI E S NG HSL S Y GRFDQY I YPYY KH D KDLNNI TEE Q A V ELLD NL WIK TL 322
Cdd:COG1882 240 L EIA EI C GF VP ANP A R TF W EA V Q WV WF VY L AAIK E Q NG AAM S L GRFDQY L YPYY ER D LEEGRL TEE E A Q ELLD CF WIK LR 319
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 323 TINKV R SQAHTFSS AG S P MYQNV TIGG Q TPD KK DA T NELSYL V L KSVAQTR LP Q PNLTVR YHKNM P KA FL DE A I EV MKL G 402
Cdd:COG1882 320 EVRFL R TPEYAELF AG Y P TWVTL TIGG M TPD GR DA V NELSYL I L ETLRNLP LP E PNLTVR WSEKL P EG FL KK A A EV ISI G 399
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 403 TG M P AFN ND EII IP SFIE KGV KE EDA YN Y SAI GCVE TA VPGK W g YRCT G MSYM N FPRI L LM A M N D GVD MTS GK RFFEGS G 482
Cdd:COG1882 400 TG S P QYE ND DLM IP MLLN KGV TL EDA RD Y GIA GCVE PM VPGK Q - MQFF G AGRI N LAKA L EY A L N N GVD EKT GK QVGPET G 478
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 483 YFK D MTS YDE LFK A IE K GMR YL TRMS V IVE N A I DLALERDV P DVLC SAL TE DCI G RGK TIK EGGA V Y D F ISGLQV G IANM 562
Cdd:COG1882 479 DPT D FLT YDE VME A FK K QLD YL ADLY V NAL N I I HYMHDKYA P EPFL SAL HD DCI E RGK DLN EGGA R Y N F GAIGIA G LSVV 558
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 563 ADSL V AIKKLVF EE KK I T PE EL WN A ILDDYTSK E S qt IQEM L I N d V PKYGND I D E VD QLV V DVYNVYI DE MK KY PNT R yg 642
Cdd:COG1882 559 ADSL S AIKKLVF DK KK V T MD EL LE A LAANFEGY E E -- LRQL L L N - A PKYGND D D Y VD EIA V ELVETFM DE IR KY KTY R -- 633
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 643 rgpi GG IRYAGTSS I SA NV GQ G YG T M ATPDGRKA HT PLA E G C SP A H AM DK K GPTAV F KSV S KLP THEI T G G V LLNQK VT P 722
Cdd:COG1882 634 ---- GG TYTLSILT I TS NV PY G KK T G ATPDGRKA GE PLA D G A SP M H GR DK N GPTAV L KSV A KLP YEKA T D G I LLNQK FS P 709
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 723 QL L SK EE NK E KIEM L IK T F F N r L K G Y H V Q Y NVV SK ETLLDAQ A HPEK HK DL I VRVAGYSA F F NV LSK AT QDDII G RTE QT 802
Cdd:COG1882 710 SA L GG EE GI E NLVS L LR T Y F D - L G G H H I Q F NVV DR ETLLDAQ K HPEK YP DL T VRVAGYSA Y F VE LSK EQ QDDII A RTE HE 788
.
gi 1473344021 803 L 803
Cdd:COG1882 789 F 789
PFL2-3
TIGR01774 glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated ...
10-803
0e+00
glycyl radical enzyme, PFL2/glycerol dehydratase family; This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Pssm-ID: 273798 [Multi-domain]
Cd Length: 786
Bit Score: 868.07
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 LTP R MNHFREEVLDKKPYICAK RA L L A TE AY K EHLN EP P I MK RA YM L KNI LE KM PI Y I EEDT LIVGN QASSN R D A P IFPE 89
Cdd:TIGR01774 2 CMD R IEKLKKALSKPVARLSVE RA R L Y TE SM K QTEG EP M I IR RA KA L AHV LE NI PI Q I LDSE LIVGN MLPNP R A A I IFPE 81
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 90 YTMEFVMN ELD LFEK R DGDV F YIT EE T K EE LR S - IAP F W ENNNLRSKGGA L LP DE VSVYME T GF F GME G k LNS G DA H L AV 168
Cdd:TIGR01774 82 GVGLWIIK ELD SLPN R PTNR F AVS EE D K RV LR E e IAP Y W QRKTIEDFAFG L MT DE MKILYT T SI F VLT G - EIA G IS H V AV 160
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 169 D Y QQV L QK GL KGYEKRVKDLKES L DLCIPENID KY Q FY K A VL IV I EAV KTFAN RY SE LA L E L A R S AE G - K R K EEL EE IA R 247
Cdd:TIGR01774 161 N Y PYL L RR GL RWLLEESERRIRA L EESGVYEGE KY S FY Q A AK IV L EAV INHIL RY AK LA E E M A A S ET G e S R R EEL LK IA E 240
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 248 V CRKV PY EK AE TF YE A I Q ST W FI Q L ILQ I ESN GH S L S Y GR F DQY I YP Y YK H D KDLNN I TE E Q A V E L L DN LWIKT LT I NKV 327
Cdd:TIGR01774 241 I CRKV AA EK PQ TF WQ A V Q LV W LV Q S ILQ Q ESN EQ S I S M GR I DQY L YP F YK K D IGEGR I DR E L A F E I L AS LWIKT NE I VPA 320
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 328 RS QAHTFSS AG S P MY Q N VTIGG QTPDKK DA T NELSYL V L KSVAQT RL P QPNL T VR YHKNM P KA FL DEAI E VMKL G T G M PA 407
Cdd:TIGR01774 321 RS SSLEQYF AG Q P TN Q A VTIGG CDIYGN DA V NELSYL M L EVTDRL RL R QPNL H VR INAGS P ES FL KRLA E AIRS G C G N PA 400
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 408 FN NDE III P S F IEK GV KEE DA YN Y SAI GCVE T A VP G KW g Y RCTGMSYM N FPRILLM A M N D GVD MTS G KR F FEGS gyf KDM 487
Cdd:TIGR01774 401 LF NDE AVV P A F KNA GV DDR DA LD Y TTD GCVE I A PF G NS - Y TSSDAALI N VAKVMEY A L N E GVD LQF G YE F GAKT --- EKP 476
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 488 TSYDE L FKAIEKGMRYLTRMS V IVE N AI D L A LERDV P DV L C S ALT EDC IGR G KT I KE GGA V Y D F ISGLQ VGIAN MA DSLV 567
Cdd:TIGR01774 477 KFLED L LEKLREQISHYVKLV V EGS N VL D Y A NAEVK P TP L L S LCV EDC FEK G VD I SR GGA R Y N F TGIQA VGIAN LG DSLV 556
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 568 AIK KL VFE EKKITPEE L WN A ILDDYTSKESQTIQEM LIN DV PKYGND I D E VD QLVVD V YNV Y ID E MKK Y P N T R ygrgpi G 647
Cdd:TIGR01774 557 AIK GA VFE QGAYSMDD L VE A LRKNFVGLTHEEKRHR LIN QS PKYGND D D A VD KYTAM V LEW Y CE E VNR Y R N P R ------ G 630
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 648 G IRY AG TSSI SANV GQ G YG T M ATPDGRKA HT PLA E G C SP AHA MD KK GPTAV FK SVSKL PTHEITG G V LLN Q K VT P QL L SK 727
Cdd:TIGR01774 631 G KFA AG CYPM SANV PF G FF T S ATPDGRKA GE PLA P G V SP STG MD RE GPTAV IN SVSKL SYENLPN G A LLN I K LS P AT L EE 710
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1473344021 728 E EN K E K IEMLIKTF F NR LK GY H V Q Y N V V SKE TL LD AQ AH PEK HKD L I VRVAGYSAFF NV LS KAT QDDII G RT EQT L 803
Cdd:TIGR01774 711 E GD K Q K LIEALRKS F ME LK VM H I Q F N I V KED TL RK AQ QE PEK YRW L V VRVAGYSAFF VE LS RPV QDDII R RT SHR L 786
PFL-like
pfam02901 Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ...
10-664
0e+00
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048
Cd Length: 647
Bit Score: 748.69
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 LTP R MNHFR E EVL -- DKK P YICAK RA L L A TE A YKE HLNEP P I - MK RA YM LK N IL EKM P I YI EE D T LIVG N Q ASSN R DAP I 86
Cdd:pfam02901 1 MWE R IDVLK E NYT ly TGD P SLSWE RA R L L TE S YKE TEGVL P V d IR RA KA LK K IL SHL P G YI RD D E LIVG L Q TDKP R KRA I 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 87 F PE YTMEF V MN ELD LFEK R DG D V F Y I T EE T K EEL R S I A P F W ENNNLRSKGGALLPD E VSVYM E T G F F g MEGKLNS G DA H L 166
Cdd:pfam02901 81 Y PE GGIRW V ED ELD YLNT R PQ D G F E I S EE D K KIF R E I F P Y W KGKTHNEGVFDAYTP E MKAAR E S G I F - TGLPDAY G RG H I 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 167 AV DY QQ VL QK GL K G YEKRVKDLKES LD l CI PE N I D K YQ FYKA VL I VIE AV KTF A N RY SE LA L ELA RS - AEG KRK E EL E EI 245
Cdd:pfam02901 160 IG DY RR VL LY GL D G LIEEKEEKLAK LD - TD PE D I E K IE FYKA MI I SCD AV IEY A E RY AR LA E ELA EQ e TDP KRK A EL L EI 238
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 246 A RV C RK VP YEK AETF Y EAIQ ST WF IQ L ILQI E S NG HSL S Y GR F DQY I YPYY KH D KDLNNI TEE Q A V EL L D NL WIK TLTIN 325
Cdd:pfam02901 239 A EI C GR VP ARP AETF Q EAIQ WF WF VY L AAVK E Q NG AAM S L GR L DQY L YPYY ER D LEEGRL TEE E A Q EL I D CF WIK LREVR 318
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 326 KV R SQAHTFSS AG SPMY QN V TIGGQ TP D KK DA T N E LSYL V L KSVAQTR LP Q PNLTVR YH K NM P KA FL DE A I EV MKL GTG M 405
Cdd:pfam02901 319 FL R TPEYNKLF AG YDPF QN L TIGGQ GR D GR DA V N K LSYL I L EALDNLP LP E PNLTVR WS K KL P EE FL KK A A EV SRK GTG S 398
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 406 P AFN ND EII IP SFIEK GV KE EDA YN Y SAI GCVE TAV PGK - WGYRC tgm SYM N FPRI L LM A M N D G V D MTS GK RFFEGS G YF 484
Cdd:pfam02901 399 P QYE ND DVM IP ALLNR GV SL EDA RD Y GIA GCVE PMK PGK e MQFFG --- ARI N LAKA L EY A L N G G R D ELT GK QVGPKT G PV 475
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 485 KDMT S YD E LFK A IE K GMR YL TRMS V IVE N A I DLALERDV P DVLC SAL TE DCI G RGK TIKE GGA V Y D F ISGLQV G I AN M AD 564
Cdd:pfam02901 476 TEFL S FE E VME A FK K QLD YL ADLY V NAL N I I HYMHDKYA P EPFL SAL HD DCI E RGK DVGI GGA R Y N F SGPQGA G L AN V AD 555
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 565 SL V AIKKLVF EE K KI T PE EL WN A ILD D YTSK E S qt IQEM L I ND V PKYGND I D E VD QLV V D V YNVYI DE MK KY P N T R ygrg 644
Cdd:pfam02901 556 SL S AIKKLVF DD K VY T LR EL ED A LAA D FEGE E E -- LRQD L L ND A PKYGND D D R VD DIA V E V VETFM DE VR KY K N Y R ---- 629
650 660
....*....|....*....|
gi 1473344021 645 pi GG IRYAGTSS I SA NV GQ G 664
Cdd:pfam02901 630 -- GG KFTPSLLT I TS NV PY G 647
pflD
PRK09983 putative formate acetyltransferase 2; Provisional
10-803
0e+00
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain]
Cd Length: 765
Bit Score: 569.07
E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 L T P R MNHFREEVLDKKPY I CAK RALL A T EAYKEHLN EP P I MK RA YMLKN ILE KMP I Y I EEDT LI V GN QASSN R DAPIF PE 89
Cdd:PRK09983 1 M T N R ISRLKTALFANTRE I SLE RALL Y T ASHRQTEG EP V I LR RA KATAY ILE HVE I S I RDEE LI A GN RTVKP R AGIMS PE 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 90 YTMEFVMN ELD L F EK R DG D V F Y I T EE T K EEL R - SIA P F WE NNNLRSKGGALLP DEV SVYME T GF F GME g KLNS G DA H LAV 168
Cdd:PRK09983 81 MDPYWLLK ELD Q F PT R PQ D R F A I S EE D K RIY R e ELF P Y WE KRSMKDFINGQMT DEV KAATS T QI F SIN - QTDK G QG H III 159
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 169 DY QQV L QK GL KGYEKRVKDLKESL dlci PEN idky Q FY K A V L IVI EA VKTFAN RY S ELA LEL A - RSAEGK R K EEL EE IA R 247
Cdd:PRK09983 160 DY PRL L NH GL GELVAQMQQHCQQQ ---- PEN ---- H FY Q A A L LLL EA SQKHIL RY A ELA ETM A a NCTDAQ R R EEL LT IA E 231
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 248 VC R KVPYE K AE TF YE A I Q ST W FIQL ILQ I ESN GH SLS Y GRFDQY IY P Y Y K hd KD L N - NITEEQAV ELL DN LW I K TLT I NK 326
Cdd:PRK09983 232 IS R HNAQH K PQ TF WQ A C Q LF W YMNI ILQ Y ESN AS SLS L GRFDQY ML P F Y Q -- AS L T q GEDPAFLK ELL ES LW V K CND I VL 309
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 327 V RS QAHTFSS AG S P MYQNVTI GG Q T PDKKD A T N E LS Y L V L KSVAQTR LPQPNL T VR YHKNMPKA FL DEAI E VMK LGTG M P 406
Cdd:PRK09983 310 L RS TSSARYF AG F P TGYTALL GG L T ENGRS A V N V LS F L C L DAYQSVQ LPQPNL G VR TNALIDTP FL MKTA E TIR LGTG I P 389
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 407 AFN NDE III P S F IEK GV KE EDA YN YS AI GCVE TAV PG K w G Y RCTGMSYM N FPRILLMAMNDG vdmtsgkrff EG S gyfk D 486
Cdd:PRK09983 390 QIF NDE VVV P A F LNR GV SL EDA RD YS VV GCVE LSI PG R - T Y GLHDIAMF N LLKVMEICLHEN ---------- EG N ---- A 454
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 487 MTS Y DE L FKA I EKGMRYLTRMS V IVE N AI D LA l E RD - V P DV L C S ALTE DC IGR G KT I KE GGA V Y D F i SG L Q - V GIAN MA D 564
Cdd:PRK09983 455 ALT Y EG L LEQ I RAKISHYITLM V EGS N IC D IG - H RD w A P VP L L S SFIS DC LEK G RD I TD GGA R Y N F - SG V Q g I GIAN LS D 532
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 565 SL V A I K KL VF EEKKITPE EL WNAILDDYTSK E SQTIQEM LIN DVP KYGNDIDEVD QLVVDVYNV Y ID E MK KY P N T R ygrg 644
Cdd:PRK09983 533 SL H A L K GM VF DQQRLSFD EL LSVLKANFATP E GEKVRAR LIN RFE KYGNDIDEVD NISAELLRH Y CK E VE KY Q N P R ---- 608
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 645 pi GG IRYA G TSSI SA N V GQ G YGTM ATPDGR K A HTP LA E - G C SP AHAM D KK GPTAV F KSVSKL PTHEITG G V LLN Q K V TP Q 723
Cdd:PRK09983 609 -- GG YFTP G SYTV SA H V PL G SVVG ATPDGR F A GEQ LA D g G L SP MLGQ D AQ GPTAV L KSVSKL DNTLLSN G T LLN V K F TP A 686
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 724 L L SK E ENKE K IEMLIKT f F NR LK GY H V Q Y NVV SKE TL LD AQ AH P EKHKD L I VRVAGYSAFF NV LSK AT QDDII G RT EQT L 803
Cdd:PRK09983 687 T L EG E AGLR K LADFLRA - F TQ LK LQ H I Q F NVV NAD TL RE AQ QR P QDYAG L V VRVAGYSAFF VE LSK EI QDDII R RT AHQ L 765
choline_CutC
TIGR04394 choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ...
10-799
1.27e-176
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain]
Cd Length: 789
Bit Score: 527.45
E-value: 1.27e-176
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 10 L T P R MNHFR E EV L DKK P Y I CAK RA LLA TE AY KE HLNE P P I MK RA YMLKNIL E KM P IY I EE D T LIVG NQASSN R DAPIF P E 89
Cdd:TIGR04394 1 P T E R LVRLK E NY L KQV P S I TIY RA RAI TE IA KE NPGM P K I LL RA KAFRKCC E TA P LV I QD D E LIVG APCGAP R AGAFS P D 80
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 90 YTMEFVMN ELD LFEK R DG D V FYI T EE T K EEL R S - I A PFWE NNN L rskggallp DE VS -- V Y M E T G FFGME G KLNSG D ahl 166
Cdd:TIGR04394 81 IAWRWLRD ELD TIGT R PQ D P FYI S EE D K KIM R E e I F PFWE GKS L --------- DE YC ed Q Y R E A G VWELS G ESFVS D --- 148
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 167 a VD Y QQ V -------------- LQ KG LKGYEKRV K DLKES L DLCI PE N IDK YQ FYK A V LIVI E A V KTF A N R Y SE L A L ELA - 231
Cdd:TIGR04394 149 - CS Y HA V ngggdsnpgydvil MK KG MLDIQAEA K EHLAE L SYEN PE D IDK IY FYK S V IDTT E G V MIY A K R L SE Y A A ELA a 227
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 232 RSAEG KRK E ELE E IA R V CRK VP YE K AE TF Y EAIQS T W FIQLI L QI E S N GHSL S Y GR F DQY I YP Y YK H D KDLNNI TE EQ A V 311
Cdd:TIGR04394 228 KEQDP KRK A ELE K IA E V NAR VP AH K PR TF W EAIQS V W TVESL L VV E E N QTGM S I GR V DQY M YP F YK A D IEAGRM TE YE A F 307
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 312 EL LDNLW IK TLTINKVR S QAHTFSS AG SPMYQ N VTI GG Q T PDKK DATN E L S YL VLKS V AQTRLP QP N L TV R Y H KNM P KAF 391
Cdd:TIGR04394 308 EL AGCML IK MSEMMWLT S EGGSKFF AG YQPFV N MCV GG V T REGG DATN D L T YL LMDA V RHVKVY QP S L AC R I H NKS P QKY 387
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 392 L DEAIE V MKL G T G M PA FNN D EII I PSFIE KGV KE EDA YN Y SAI GCVE tav P G K W G -- Y RC T GMS Y MNF P RILLMAM N D GV 469
Cdd:TIGR04394 388 L KKIVD V VRA G M G F PA CHF D DAH I KMMLA KGV SI EDA RD Y CLM GCVE --- P Q K S G rl Y QW T STA Y TQW P ICIELVL N H GV 464
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 470 DMTS GK RFFEGS G YFKDMTS Y DELFK A IEKGMR Y L T RM S V i V ENA I DLALE RD V - P DV L C S ALT E D C IGR GK TIKE GGA V 548
Cdd:TIGR04394 465 PLWY GK QVCPDT G DLSQFDT Y EKFDA A VKEQIK Y I T KW S A - V ATV I SQRVH RD L a P KP L M S LMY E G C MEK GK DVSA GGA M 543
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 549 Y D F IS G L - QV G I A NM ADS LV AIKKLV FEE KK I T P E E L WN A ILDDYTSK E s Q TIQEM L in D V PKYGND I D EV D QLVV D VY N 627
Cdd:TIGR04394 544 Y N F GP G V v WS G L A TY ADS MA AIKKLV YDD KK Y T L E Q L NE A LKANFEGY E - Q IRADC L -- D A PKYGND D D YA D LIAA D LV N 620
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 628 VYID E MK KY p N T R Y grgpig GIRYA GT S SIS A N VGQ G YG T M A TPD GR K A H TPL AE G C SP AHAM D K KGPTA VF KSVSK LPT 707
Cdd:TIGR04394 621 FTER E HR KY - K T L Y ------ SHLSH GT L SIS N N TPF G QL T G A SAN GR L A W TPL SD G I SP TQGA D F KGPTA II KSVSK MAN 693
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 708 HEITG G VLL N Q K VTPQ LL SKE E NKEKIEM L IK T f FNR L KGYHV Q Y N VVSK ETLLDAQ A HPEK HK DL I VRVAGYSAFF NV L 787
Cdd:TIGR04394 694 DSMNI G MVH N F K LMSG LL DTP E GENGLIT L LR T - ASI L GNGEM Q F N YLDN ETLLDAQ Q HPEK YR DL V VRVAGYSAFF VE L 772
810
....*....|..
gi 1473344021 788 S K AT QD D II G RT 799
Cdd:TIGR04394 773 C K DV QD E II S RT 784
PFL1
cd01678 Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ...
257-799
4.49e-66
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain]
Cd Length: 738
Bit Score: 234.18
E-value: 4.49e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 257 A ETFY EAIQ S T W F IQ L ILQI E S NG HSL S Y GR FDQYIYP Y YKH D KDLNN ITE EQ A V EL L D NLWI K TLTINKV R SQAHTFSS 336
Cdd:cd01678 235 A TNAQ EAIQ W T Y F GY L AAIK E Q NG AAM S L GR VSTFLDI Y IER D LKAGT ITE AE A Q EL I D QFIM K LRMVRFL R TPEYNELF 314
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 337 A G S P MYQNVT IGG QTP D KKDATNEL S YLV L KSV aq TR L --- P Q PNLTV RYHKNM P KA F LDEAIE v MKLG T GMPAFN ND EI 413
Cdd:cd01678 315 S G D P TWVTES IGG MGN D GRSLVTKT S FRF L NTL -- YN L gpa P E PNLTV LWSEKL P EN F KRFCAK - VSID T SSIQYE ND DL 391
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 414 II P SF iekgvkeed AYNYSA I G C - V ETAVP GK ---- W G Y R C tgmsym N FPRI LL M A M N D G V D MTS G KRFFEGSGYFKDMT 488
Cdd:cd01678 392 MR P DW --------- GGDDYG I A C c V SAMRI GK qmqf F G A R A ------ N LAKA LL Y A I N G G R D EKT G DQVGPDIEPITSDY 456
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 489 - S YDE LFKAIE K G M RY L TRMS V IVE N A I DLALERDVPDV L CS AL TEDCIG R gk T IKE G gavydf I S GL qvgi ANM ADSL V 567
Cdd:cd01678 457 l D YDE VMENYD K S M DW L ADTY V NAL N I I HYMHDKYAYEA L QM AL HDTDVR R -- T MAF G ------ I A GL ---- SVA ADSL S 524
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 568 AIK K lvfee K K IT P EELWNAILD D YTSK esqtiqemli N D V P K YGND I D EV D QLV V D V YNVYIDEMK K YPNT R YGR gpig 647
Cdd:cd01678 525 AIK Y ----- A K VK P IRDEDGLAV D FEIE ---------- G D F P R YGND D D RA D DIA V W V VKTFMNKLR K HKTY R NAE ---- 585
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 648 giry AGT S -- S I SA NV GQ G YG T MA TPDGR K A HT P L A E G CS P A H AM DKKG PT A VFK SV S KLP THEITG G VLLNQKVT P QL L 725
Cdd:cd01678 586 ---- PTQ S vl T I TS NV VY G KK T GN TPDGR R A GE P F A P G AN P M H GR DKKG AL A SLA SV A KLP YRDAND G ISNTFSIV P NA L 661
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1473344021 726 S K -- EE NKEKIEMLIKTF F NR l K G Y H VQY NV VSK ETLLDA QA HPEK HKD L IV RV A GY SAF F NV L SKAT Q D D I I G RT 799
Cdd:cd01678 662 G K td EE RIDNLVGILDGY F TK - G G H H LNV NV LNR ETLLDA ME HPEK YPQ L TI RV S GY AVN F VK L TREQ Q L D V I S RT 736
Gly_radical
pfam01228 Glycine radical;
680-785
2.91e-41
Glycine radical;
Pssm-ID: 426140 [Multi-domain]
Cd Length: 106
Bit Score: 146.16
E-value: 2.91e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 680 L A E G C SP A H AM D KK GPTAV FK SV S K LPTHEITG G VL LNQK VT P QL L -- SK EE NKEKIEM LI K T F F N rl K G Y H V Q Y NVV SK 757
Cdd:pfam01228 1 V A P G I SP S H GA D FE GPTAV LN SV G K IDYEVELD G IS LNQK FL P AV L gy YD EE GYANLNT LI D T Y F E -- G G H H L Q F NVV DR 78
90 100
....*....|....*....|....*...
gi 1473344021 758 ETL L DAQ A HPEK HK DL I VRV A GYSA F F N 785
Cdd:pfam01228 79 ETL P DAQ K HPEK YP DL T VRV S GYSA N F V 106
RNR_PFL
cd00576 Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ...
241-439
1.28e-18
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain]
Cd Length: 401
Bit Score: 89.13
E-value: 1.28e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 241 ELEEIARVCR K VPY E K A ETFY EAIQ S T WF I QLILQIES NG HSL S YG R FDQYIY PY YKH D KDLNNI TE EQ AVE LL D NLWIK 320
Cdd:cd00576 29 DYGDSLDPGI K GVN E T A KSIN EAIQ K T YQ I IALAASNQ NG GGV S FA R ASSILS PY GSR D YAKGSG TE TD AVE AA D AFNLA 108
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1473344021 321 TLTI nkvrsqaht FSSA G SPMYQNVT IGG QTPD K -- K DATNE L SYLVLKSVAQTR L PQ PNL T VR YHKNM P k AF L DE A I E V 398
Cdd:cd00576 109 LKEV --------- GQGN G RTGAATGF IGG VHKG K gd K ISQEF L NLALANGGEGIP L NF PNL S VR VSSDK P - GI L VK A V E L 178
170 180 190 200
....*....|....*....|....*....|....*....|....*..
gi 1473344021 399 MK L ------ G TG M P AFN NDE IIIPSFIEKGVKE E D A Y N Y S AIGCV E T 439
Cdd:cd00576 179 KQ L iaeear K TG S P GIF NDE LCNLVSLNLARIM E K A I N G S MDVVL E E 225
PRK11127
PRK11127 autonomous glycyl radical cofactor GrcA; Provisional
747-799
3.48e-16
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain]
Cd Length: 127
Bit Score: 75.65
E-value: 3.48e-16
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|...
gi 1473344021 747 G Y H VQY NV VSK ETL L DA QA HPEK HKD L IV RV A GY SAF FN V L SKAT Q D D I I G RT 799
Cdd:PRK11127 70 G Q H LNV NV LRR ETL E DA VK HPEK YPQ L TI RV S GY AVR FN S L TPEQ Q R D V I A RT 122
Blast search parameters
Data Source:
Precalculated data, version = cdd.v.3.21
Preset Options: Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
