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Conserved domains on  [gi|1482688674|ref|WP_119753736|]
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type II toxin-antitoxin system HigA family antitoxin [Chryseolinea soli]

Protein Classification

type II toxin-antitoxin system HigA family antitoxin( domain architecture ID 10009358)

type II toxin-antitoxin system HigA family antitoxin similar to Escherichia coli HigA, the antitoxin component of the HigB-HigA TA system; binds to HigB to inhibit its mRNA interferase activity

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HigA COG5499
Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH ...
 1-129 1.52e-19

Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH domain [Defense mechanisms];


:

Pssm-ID: 444250  Cd Length: 127  Bit Score: 77.95  E-value: 1.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1482688674   1 MITKIRTAAEYKLVMKSIEAMLQTAtkkggfhKLSKDESATLAKLSKLAETYEDKDlqlMPIKPKTIQEAVEFKRAEKRL 80
Cdd:COG5499    10 PLKPIRTEADYEAALALIEELLDAD-------ELGTPEGDLLDLLATLIEAYEAKH---YPIPPPDPIEALKFLMEQHGL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1482688674  81 TQAKLAKKLGiGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLTHA 129
Cdd:COG5499    80 TQKDLAPEIG-SKSRVSEILNGKRSLTLEMIRKLAERFGIPAELFIQPY 127
 
Name Accession Description Interval E-value
HigA COG5499
Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH ...
 1-129 1.52e-19

Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH domain [Defense mechanisms];


Pssm-ID: 444250  Cd Length: 127  Bit Score: 77.95  E-value: 1.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1482688674   1 MITKIRTAAEYKLVMKSIEAMLQTAtkkggfhKLSKDESATLAKLSKLAETYEDKDlqlMPIKPKTIQEAVEFKRAEKRL 80
Cdd:COG5499    10 PLKPIRTEADYEAALALIEELLDAD-------ELGTPEGDLLDLLATLIEAYEAKH---YPIPPPDPIEALKFLMEQHGL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1482688674  81 TQAKLAKKLGiGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLTHA 129
Cdd:COG5499    80 TQKDLAPEIG-SKSRVSEILNGKRSLTLEMIRKLAERFGIPAELFIQPY 127
HTH_XRE cd00093
Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the ...
 69-125 3.38e-09

Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the xenobiotic response element family of transcriptional regulators.


Pssm-ID: 238045 [Multi-domain]  Cd Length: 58  Bit Score: 49.47  E-value: 3.38e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1482688674  69 EAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:cd00093     2 ERLKELRKEKGLTQEELAEKLGVSRSTISRIENGKRNPSLETLEKLAKALGVSLDEL 58
HTH_XRE smart00530
Helix-turn-helix XRE-family like proteins;
75-125 1.03e-08

Helix-turn-helix XRE-family like proteins;


Pssm-ID: 197775 [Multi-domain]  Cd Length: 56  Bit Score: 47.90  E-value: 1.03e-08
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1482688674   75 RAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:smart00530   6 REEKGLTQEELAEKLGVSRSTLSRIENGKRKPSLETLKKLAKALGVSLDEL 56
HTH_3 pfam01381
Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and ...
 74-125 1.84e-06

Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and CI. Within the protein Swiss:Q5F9C2, the full protein fold incorporates a helix-turn-helix motif, but the function of this member is unlikely to be that of a DNA-binding regulator, the function of most other members, so is not necessarily characteriztic of the whole family.


Pssm-ID: 460181 [Multi-domain]  Cd Length: 55  Bit Score: 42.14  E-value: 1.84e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1482688674  74 KRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:pfam01381   4 LREELGLSQEELAEKLGVSRSTISKIENGKREPSLETLKKLAEALGVSLDEL 55
PRK09706 PRK09706
transcriptional repressor DicA; Reviewed
 62-126 4.28e-03

transcriptional repressor DicA; Reviewed


Pssm-ID: 182039 [Multi-domain]  Cd Length: 135  Bit Score: 34.83  E-value: 4.28e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1482688674  62 IKPKTIQEAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLL 126
Cdd:PRK09706    1 MKNLTLGQRIRYRRKQLKLSQRSLAKAVKVSHVSISQWERDETEPTGKNLFALAKALQCSPTWLL 65
antidote_HigA TIGR02607
addiction module antidote protein, HigA family; Members of this family form a distinct clade ...
 79-126 9.93e-03

addiction module antidote protein, HigA family; Members of this family form a distinct clade within the larger family HTH_3 of helix-turn-helix proteins, described by pfam01381. Members of this clade are strictly bacterial and nearly always shorter than 110 amino acids. This family includes the characterized member HigA, without which the killer protein HigB cannot be cloned. The hig (host inhibition of growth) system is noted to be unusual in that killer protein is uncoded by the upstream member of the gene pair. [Regulatory functions, DNA interactions, Regulatory functions, Protein interactions, Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274228 [Multi-domain]  Cd Length: 78  Bit Score: 32.97  E-value: 9.93e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1482688674  79 RLTQAKLAKKLGIGAPKLSQILSGKR--EPDVVfLKaLHKELNIDAEFLL 126
Cdd:TIGR02607  18 GLSVRALAKALGVSRSTLSRIVNGRAaiTADMA-LR-LAKALGTSPEFWL 65
 
Name Accession Description Interval E-value
HigA COG5499
Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH ...
 1-129 1.52e-19

Antitoxin component HigA of the HigAB toxin-antitoxin module, contains an N-terminal HTH domain [Defense mechanisms];


Pssm-ID: 444250  Cd Length: 127  Bit Score: 77.95  E-value: 1.52e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1482688674   1 MITKIRTAAEYKLVMKSIEAMLQTAtkkggfhKLSKDESATLAKLSKLAETYEDKDlqlMPIKPKTIQEAVEFKRAEKRL 80
Cdd:COG5499    10 PLKPIRTEADYEAALALIEELLDAD-------ELGTPEGDLLDLLATLIEAYEAKH---YPIPPPDPIEALKFLMEQHGL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1482688674  81 TQAKLAKKLGiGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLTHA 129
Cdd:COG5499    80 TQKDLAPEIG-SKSRVSEILNGKRSLTLEMIRKLAERFGIPAELFIQPY 127
HipB COG1396
Transcriptional regulator, contains XRE-family HTH domain [Transcription];
60-127 4.66e-11

Transcriptional regulator, contains XRE-family HTH domain [Transcription];


Pssm-ID: 441006 [Multi-domain]  Cd Length: 83  Bit Score: 55.00  E-value: 4.66e-11
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1482688674  60 MPIKPKTIQEAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLT 127
Cdd:COG1396     1 MSTLKKALGERLRELRKARGLTQEELAERLGVSRSTISRIERGRRNPSLETLLKLAKALGVSLDELLG 68
HTH_XRE cd00093
Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the ...
 69-125 3.38e-09

Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the xenobiotic response element family of transcriptional regulators.


Pssm-ID: 238045 [Multi-domain]  Cd Length: 58  Bit Score: 49.47  E-value: 3.38e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1482688674  69 EAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:cd00093     2 ERLKELRKEKGLTQEELAEKLGVSRSTISRIENGKRNPSLETLEKLAKALGVSLDEL 58
HTH_XRE smart00530
Helix-turn-helix XRE-family like proteins;
75-125 1.03e-08

Helix-turn-helix XRE-family like proteins;


Pssm-ID: 197775 [Multi-domain]  Cd Length: 56  Bit Score: 47.90  E-value: 1.03e-08
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1482688674   75 RAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:smart00530   6 REEKGLTQEELAEKLGVSRSTLSRIENGKRKPSLETLKKLAKALGVSLDEL 56
XRE COG1476
DNA-binding transcriptional regulator, XRE-family HTH domain [Transcription];
75-127 1.32e-06

DNA-binding transcriptional regulator, XRE-family HTH domain [Transcription];


Pssm-ID: 441085 [Multi-domain]  Cd Length: 68  Bit Score: 42.91  E-value: 1.32e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1482688674  75 RAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLT 127
Cdd:COG1476    13 RKERGLTQEELAELLGVSRQTISAIENGKYNPSLELALKIARALGVSLEELFS 65
AF2118 COG3620
Predicted transcriptional regulator, contains an XRE-type HTH domain (archaeal members contain ...
 66-126 1.44e-06

Predicted transcriptional regulator, contains an XRE-type HTH domain (archaeal members contain CBS pair) [Transcription];


Pssm-ID: 442838 [Multi-domain]  Cd Length: 95  Bit Score: 43.47  E-value: 1.44e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1482688674  66 TIQEAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLL 126
Cdd:COG3620    17 TLGEALRLMRKELGLSQLPVAELVGVSQSDILRIESGKRDPTVSTLEKIAEALGKELSAVL 77
HTH_3 pfam01381
Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and ...
 74-125 1.84e-06

Helix-turn-helix; This large family of DNA binding helix-turn helix proteins includes Cro and CI. Within the protein Swiss:Q5F9C2, the full protein fold incorporates a helix-turn-helix motif, but the function of this member is unlikely to be that of a DNA-binding regulator, the function of most other members, so is not necessarily characteriztic of the whole family.


Pssm-ID: 460181 [Multi-domain]  Cd Length: 55  Bit Score: 42.14  E-value: 1.84e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1482688674  74 KRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFL 125
Cdd:pfam01381   4 LREELGLSQEELAEKLGVSRSTISKIENGKREPSLETLKKLAEALGVSLDEL 55
aMBF1 COG1813
Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and ...
 75-123 3.83e-06

Archaeal ribosome-binding protein aMBF1, putative translation factor, contains Zn-ribbon and HTH domains [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441418 [Multi-domain]  Cd Length: 70  Bit Score: 41.85  E-value: 3.83e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1482688674  75 RAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAE 123
Cdd:COG1813    21 REARGLSQEELAEKLGVSESTIRRIERGEATPSLDTLRKLEKALGISLA 69
HTH_19 pfam12844
Helix-turn-helix domain; Members of this family contains a DNA-binding helix-turn-helix domain. ...
 69-127 9.61e-06

Helix-turn-helix domain; Members of this family contains a DNA-binding helix-turn-helix domain. This family contains many example antitoxins from bacterial toxin-antitoxin systems. These antitoxins are likely to be DNA-binding domains.


Pssm-ID: 463728 [Multi-domain]  Cd Length: 64  Bit Score: 40.73  E-value: 9.61e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1482688674  69 EAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLLT 127
Cdd:pfam12844   2 ERLRKAREERGLTQEELAERLGISRSQLSAIENGKSVPPAETLYKIAELLGVPANWLLQ 60
VapI COG3093
Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense ...
 77-126 4.26e-05

Plasmid maintenance system antidote protein VapI, contains XRE-type HTH domain [Defense mechanisms];


Pssm-ID: 442327 [Multi-domain]  Cd Length: 87  Bit Score: 39.41  E-value: 4.26e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1482688674  77 EKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLL 126
Cdd:COG3093    20 PLGLSQTELAKALGVSRQRISEILNGKRAITADTALRLARAFGTSAEFWL 69
COG5606 COG5606
Predicted DNA-binding protein, XRE-type HTH domain [General function prediction only];
76-103 3.55e-03

Predicted DNA-binding protein, XRE-type HTH domain [General function prediction only];


Pssm-ID: 444337  Cd Length: 105  Bit Score: 34.47  E-value: 3.55e-03
                          10        20
                  ....*....|....*....|....*...
gi 1482688674  76 AEKRLTQAKLAKKLGIGAPKLSQILSGK 103
Cdd:COG5606    42 KERGLTQAEAAELLGITQPRVSDLLRGK 69
YiaG COG2944
DNA-binding transcriptional regulator YiaG, XRE-type HTH domain [Transcription];
60-115 3.75e-03

DNA-binding transcriptional regulator YiaG, XRE-type HTH domain [Transcription];


Pssm-ID: 442187 [Multi-domain]  Cd Length: 64  Bit Score: 33.75  E-value: 3.75e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1482688674  60 MPIKPKTIQEaVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALH 115
Cdd:COG2944     1 MTKKPLTPEE-IRALRERLGLSQAEFAALLGVSVSTVRRWEQGRRKPSGAALKLLR 55
PRK09706 PRK09706
transcriptional repressor DicA; Reviewed
 62-126 4.28e-03

transcriptional repressor DicA; Reviewed


Pssm-ID: 182039 [Multi-domain]  Cd Length: 135  Bit Score: 34.83  E-value: 4.28e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1482688674  62 IKPKTIQEAVEFKRAEKRLTQAKLAKKLGIGAPKLSQILSGKREPDVVFLKALHKELNIDAEFLL 126
Cdd:PRK09706    1 MKNLTLGQRIRYRRKQLKLSQRSLAKAVKVSHVSISQWERDETEPTGKNLFALAKALQCSPTWLL 65
antidote_HigA TIGR02607
addiction module antidote protein, HigA family; Members of this family form a distinct clade ...
 79-126 9.93e-03

addiction module antidote protein, HigA family; Members of this family form a distinct clade within the larger family HTH_3 of helix-turn-helix proteins, described by pfam01381. Members of this clade are strictly bacterial and nearly always shorter than 110 amino acids. This family includes the characterized member HigA, without which the killer protein HigB cannot be cloned. The hig (host inhibition of growth) system is noted to be unusual in that killer protein is uncoded by the upstream member of the gene pair. [Regulatory functions, DNA interactions, Regulatory functions, Protein interactions, Mobile and extrachromosomal element functions, Other]


Pssm-ID: 274228 [Multi-domain]  Cd Length: 78  Bit Score: 32.97  E-value: 9.93e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1482688674  79 RLTQAKLAKKLGIGAPKLSQILSGKR--EPDVVfLKaLHKELNIDAEFLL 126
Cdd:TIGR02607  18 GLSVRALAKALGVSRSTLSRIVNGRAaiTADMA-LR-LAKALGTSPEFWL 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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