P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a hydrophobic residue). The Walker A and B motifs bind the beta-gamma phosphate moiety of the bound nucleotide (typically ATP or GTP) and the Mg2+ cation, respectively. The P-loop NTPases are involved in diverse cellular functions, and they can be divided into two major structural classes: the KG (kinase-GTPase) class which includes Ras-like GTPases and its circularly permutated YlqF-like; and the ASCE (additional strand catalytic E) class which includes ATPase Binding Cassette (ABC), DExD/H-like helicases, 4Fe-4S iron sulfur cluster binding proteins of NifH family, RecA-like F1-ATPases, and ATPases Associated with a wide variety of Activities (AAA). Also included are a diverse set of nucleotide/nucleoside kinase families.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1577416168 125 NLRKLNQKFDATVIGQENAKIQIIQSLfpltLRSR-------KKPVVLLLYGRSGIGKTETAKLIADVI 186
Cdd:cd19499     1 KLLNLEERLHERVVGQDEAVKAVSDAI----RRARaglsdpnRPIGSFLFLGPTGVGKTELAKALAELL 65

Chaperone protein ClpB/Hsp104 subfamily; Bacterial Caseinolytic peptidase B (ClpB) and eukaryotic Heat shock protein 104 (Hsp104) are ATP-dependent molecular chaperones and essential proteins of the heat-shock response. ClpB/Hsp104 ATPases, in concert with the DnaK/Hsp70 chaperone system, disaggregate and reactivate aggregated proteins. This RecA-like_ClpB_Hsp104_like subfamily belongs to the RecA-like NTPase family which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1577416168 125 NLRKLNQKFDATVIGQENAKIQIIQSLfpltLRSR-------KKPVVLLLYGRSGIGKTETAKLIADVI 186
Cdd:cd19499     1 KLLNLEERLHERVVGQDEAVKAVSDAI----RRARaglsdpnRPIGSFLFLGPTGVGKTELAKALAELL 65

Zeta toxin; This family consists of several bacterial zeta toxin proteins. Zeta toxin is thought to be part of a postregulational killing system in bacteria. It relies on antitoxin/toxin systems that secure stable inheritance of low and medium copy number plasmids during cell division and kill cells that have lost the plasmid.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1577416168 158 SRKKPVVLLLYGRSGIGKTETAKLIAD---------VIGEPLFRKQFSMY---------QNSQFaTYLFGGKLLKKslIL 219
Cdd:pfam06414   7 SQERPKAILLGGQPGAGKTELARALLDelgrqgnvvRIDPDDFRELHPHYrelqaadpkTASEY-TQPDASRWVEK--LL 83

                  ....*.
gi 1577416168 220 QLAIKR 225
Cdd:pfam06414  84 QHAIEN 89

Chaperone protein ClpB/Hsp104 subfamily; Bacterial Caseinolytic peptidase B (ClpB) and eukaryotic Heat shock protein 104 (Hsp104) are ATP-dependent molecular chaperones and essential proteins of the heat-shock response. ClpB/Hsp104 ATPases, in concert with the DnaK/Hsp70 chaperone system, disaggregate and reactivate aggregated proteins. This RecA-like_ClpB_Hsp104_like subfamily belongs to the RecA-like NTPase family which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1577416168 125 NLRKLNQKFDATVIGQENAKIQIIQSLfpltLRSR-------KKPVVLLLYGRSGIGKTETAKLIADVI 186
Cdd:cd19499     1 KLLNLEERLHERVVGQDEAVKAVSDAI----RRARaglsdpnRPIGSFLFLGPTGVGKTELAKALAELL 65

lon protease homolog 2 peroxisomal; Lon protease (also known as Lon peptidase) is an evolutionarily conserved ATP-dependent serine protease, present in bacteria and eukaryotic mitochondria and peroxisomes, which mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Lon protease is both an ATP-dependent peptidase and a protein-activated ATPase. This RecA-like Lon domain subfamily belongs to the RecA-like NTPase family which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1577416168 126 LRKLNQKFDATVIGQENAKIQIIQSLFPLTLRSRKKPVVLLLYGRSGIGKTETAKLIADVIGEPLFR 192
Cdd:cd19500     1 IKKARKVLDADHYGLEDVKERILEYLAVRKLKGSMKGPILCLVGPPGVGKTSLGKSIARALGRKFVR 67

ATP-dependent Clp protease, ATP-binding subunit ClpA [Posttranslational modification, protein turnover, chaperones];

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1577416168 137 VIGQENAKIQIIQSLfpltLRSR------KKPV-VLLLYGRSGIGKTETAKLIAD 184
Cdd:COG0542   551 VIGQDEAVEAVADAI----RRSRaglkdpNRPIgSFLFLGPTGVGKTELAKALAE 601

ATP-dependent protease ATPase subunit HslU; HslU is a component of the ATP-dependent protease HslVU. In HslVU, HslU ATPase serves to unfold and translocate protein substrate, and the HslV protease degrades the unfolded proteins. This RecA-like_HslU subfamily belongs to the RecA-like NTPase family which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1577416168 127 RKLNQKFDATVIGQENAKI--------QIIQSLFPLTLRSRKKPVVLLLYGRSGIGKTETAKLIADVIGEP 189
Cdd:cd19498     3 REIVSELDKYIIGQDEAKRavaialrnRWRRMQLPEELRDEVTPKNILMIGPTGVGKTEIARRLAKLAGAP 73

ATP-dependent Clp protease ATP-binding subunit; Provisional

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1577416168 123 KTNLRKLNQKFDATVIGQENAkIQIIQSLFPLT---LRSRKKPV-VLLLYGRSGIGKTETAKLIADVIGEPLFRKQFSMY 198
Cdd:PRK11034  446 RDTLKNLGDRLKMLVFGQDKA-IEALTEAIKMSragLGHEHKPVgSFLFAGPTGVGKTEVTVQLSKALGIELLRFDMSEY 524

                          90
                  ....*....|.
gi 1577416168 199 QNSQFATYLFG 209
Cdd:PRK11034  525 MERHTVSRLIG 535