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Conserved domains on  [gi|1698258413|ref|WP_141551348|]
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HNH endonuclease [Bacillus cereus]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Nuc_N pfam14448
Nuclease N terminal; This is a conserved short region that is found in many bacterial ...
10-67 1.69e-26

Nuclease N terminal; This is a conserved short region that is found in many bacterial polymorphic toxin proteins. It is often located before C-terminal nuclease domains.


:

Pssm-ID: 373075  Cd Length: 58  Bit Score: 97.79  E-value: 1.69e-26
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1698258413  10 ADSVSPIFKGVEILPDGSVVRSGTNYSGKFQEAHDASKASIQSRISNLESGGVKGTGN 67
Cdd:pfam14448   1 ADSVSDIARGAEAGADGTLVRKGTNYSAHVNDAHKASKAKIQSRISRLEKEGIKGTGS 58
HNHc super family cl00083
HNH nucleases; HNH endonuclease signature which is found in viral, prokaryotic, and eukaryotic ...
159-255 7.70e-21

HNH nucleases; HNH endonuclease signature which is found in viral, prokaryotic, and eukaryotic proteins. The alignment includes members of the large group of homing endonucleases, yeast intron 1 protein, MutS, as well as bacterial colicins, pyocins, and anaredoxins.


The actual alignment was detected with superfamily member pfam12639:

Pssm-ID: 469607  Cd Length: 96  Bit Score: 84.44  E-value: 7.70e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1698258413 159 YDVLGFLIFKGdnlKFTLKLKEEYYVMKDTDQFKECTRILKKAIEEGKVSRELFTPQQLNQIEKGRaRIKGLTWHHHQVP 238
Cdd:pfam12639   3 FDKEGFPDFSS---VAKVQLPIELMKASRDVQFKIANKALKEEVANDPELANQFTKEQLEGIENGK-TPEGYTWHHHQDT 78
                          90
                  ....*....|....*...
gi 1698258413 239 GKMQLVLTDIHG-VNHLG 255
Cdd:pfam12639  79 GTMQLVPTEIHDkTGHTG 96
 
Name Accession Description Interval E-value
Nuc_N pfam14448
Nuclease N terminal; This is a conserved short region that is found in many bacterial ...
10-67 1.69e-26

Nuclease N terminal; This is a conserved short region that is found in many bacterial polymorphic toxin proteins. It is often located before C-terminal nuclease domains.


Pssm-ID: 373075  Cd Length: 58  Bit Score: 97.79  E-value: 1.69e-26
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1698258413  10 ADSVSPIFKGVEILPDGSVVRSGTNYSGKFQEAHDASKASIQSRISNLESGGVKGTGN 67
Cdd:pfam14448   1 ADSVSDIARGAEAGADGTLVRKGTNYSAHVNDAHKASKAKIQSRISRLEKEGIKGTGS 58
Colicin-DNase pfam12639
DNase/tRNase domain of colicin-like bacteriocin; Colicin-like bacteriocins are complex ...
159-255 7.70e-21

DNase/tRNase domain of colicin-like bacteriocin; Colicin-like bacteriocins are complex structures with an N-terminal beta-barrel translocation domain (pfam09000), a long double-alpha-helical receptor-binding domain (pfam11570) and this C-terminal RNAse/DNase domain with endonuclease activity. Their competitor bacteriocidal action is by a process that involves binding to a surface receptor, entering the cell, and, finally, killing it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3 reveals a Y-shaped molecule with the receptor binding domain forming a 100 Angstrom long stalk and the two globular heads of the translocation domain and this catalytic domain comprising the two arms.


Pssm-ID: 432688  Cd Length: 96  Bit Score: 84.44  E-value: 7.70e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1698258413 159 YDVLGFLIFKGdnlKFTLKLKEEYYVMKDTDQFKECTRILKKAIEEGKVSRELFTPQQLNQIEKGRaRIKGLTWHHHQVP 238
Cdd:pfam12639   3 FDKEGFPDFSS---VAKVQLPIELMKASRDVQFKIANKALKEEVANDPELANQFTKEQLEGIENGK-TPEGYTWHHHQDT 78
                          90
                  ....*....|....*...
gi 1698258413 239 GKMQLVLTDIHG-VNHLG 255
Cdd:pfam12639  79 GTMQLVPTEIHDkTGHTG 96
 
Name Accession Description Interval E-value
Nuc_N pfam14448
Nuclease N terminal; This is a conserved short region that is found in many bacterial ...
10-67 1.69e-26

Nuclease N terminal; This is a conserved short region that is found in many bacterial polymorphic toxin proteins. It is often located before C-terminal nuclease domains.


Pssm-ID: 373075  Cd Length: 58  Bit Score: 97.79  E-value: 1.69e-26
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1698258413  10 ADSVSPIFKGVEILPDGSVVRSGTNYSGKFQEAHDASKASIQSRISNLESGGVKGTGN 67
Cdd:pfam14448   1 ADSVSDIARGAEAGADGTLVRKGTNYSAHVNDAHKASKAKIQSRISRLEKEGIKGTGS 58
Colicin-DNase pfam12639
DNase/tRNase domain of colicin-like bacteriocin; Colicin-like bacteriocins are complex ...
159-255 7.70e-21

DNase/tRNase domain of colicin-like bacteriocin; Colicin-like bacteriocins are complex structures with an N-terminal beta-barrel translocation domain (pfam09000), a long double-alpha-helical receptor-binding domain (pfam11570) and this C-terminal RNAse/DNase domain with endonuclease activity. Their competitor bacteriocidal action is by a process that involves binding to a surface receptor, entering the cell, and, finally, killing it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3 reveals a Y-shaped molecule with the receptor binding domain forming a 100 Angstrom long stalk and the two globular heads of the translocation domain and this catalytic domain comprising the two arms.


Pssm-ID: 432688  Cd Length: 96  Bit Score: 84.44  E-value: 7.70e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1698258413 159 YDVLGFLIFKGdnlKFTLKLKEEYYVMKDTDQFKECTRILKKAIEEGKVSRELFTPQQLNQIEKGRaRIKGLTWHHHQVP 238
Cdd:pfam12639   3 FDKEGFPDFSS---VAKVQLPIELMKASRDVQFKIANKALKEEVANDPELANQFTKEQLEGIENGK-TPEGYTWHHHQDT 78
                          90
                  ....*....|....*...
gi 1698258413 239 GKMQLVLTDIHG-VNHLG 255
Cdd:pfam12639  79 GTMQLVPTEIHDkTGHTG 96
WHH pfam14414
A nuclease of the HNH/ENDO VII superfamily with conserved WHH; WHH is a predicted nuclease of ...
228-263 6.65e-09

A nuclease of the HNH/ENDO VII superfamily with conserved WHH; WHH is a predicted nuclease of the HNH/ENDO VII superfamily of the treble clef fold. The name is derived from the conserved motif WHH. It is found in bacterial polymorphic toxin systems and functions as a toxin module. WHH is the shortest version of HNH nuclease families. Like AHH and LHH, the WHH nuclease contains 4 conserved histidines of which the first one is predicted to bind a metal-ion and other three ones are involved in activation of water molecule for hydrolysis.


Pssm-ID: 433943  Cd Length: 43  Bit Score: 50.47  E-value: 6.65e-09
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1698258413 228 KGLTWHHHQVPGKMQLVLTDIH-GVNHLGGNKLWGEG 263
Cdd:pfam14414   7 KGYTWHHLDDTGTMQLVPEELHnATPHTGGVSLWKKG 43
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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