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Conserved domains on  [gi|1819399233|ref|WP_165096982|]
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hydroxyisourate hydrolase [Paenibacillus apii]

Protein Classification

hydroxyisourate hydrolase( domain architecture ID 10006399)

hydroxyisourate hydrolase catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) in the second step of a three-step ureide pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HiuH COG2351
5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide ...
 3-116 7.03e-57

5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide transport and metabolism];


:

Pssm-ID: 441918  Cd Length: 111  Bit Score: 171.47  E-value: 7.03e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAplLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:COG2351     2 GRLSTHVLDTARGRPAAGVRVELYRLDGDGWTLLAEGVTNADGRIDA--LGGEALAAGTYRLVFDTGDYFAA--RGVPPF 77

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:COG2351    78 LPEVPVRFGIADPEEHYHVPLLLSPWGYSTYRGS 111
 
Name Accession Description Interval E-value
HiuH COG2351
5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide ...
 3-116 7.03e-57

5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide transport and metabolism];


Pssm-ID: 441918  Cd Length: 111  Bit Score: 171.47  E-value: 7.03e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAplLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:COG2351     2 GRLSTHVLDTARGRPAAGVRVELYRLDGDGWTLLAEGVTNADGRIDA--LGGEALAAGTYRLVFDTGDYFAA--RGVPPF 77

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:COG2351    78 LPEVPVRFGIADPEEHYHVPLLLSPWGYSTYRGS 111
Transthyretin pfam00576
HIUase/Transthyretin family; This family includes transthyretin that is a thyroid ...
 5-115 3.75e-56

HIUase/Transthyretin family; This family includes transthyretin that is a thyroid hormone-binding protein that transports thyroxine from the bloodstream to the brain. However, most of the sequences listed in this family do not bind thyroid hormones. They are actually enzymes of the purine catabolism that catalyze the conversion of 5-hydroxyisourate (HIU) to OHCU. HIU hydrolysis is the original function of the family and is conserved from bacteria to mammals; transthyretins arose by gene duplications in the vertebrate lineage. HIUases are distinguished in the alignment from the conserved C-terminal YRGS sequence.


Pssm-ID: 459857  Cd Length: 108  Bit Score: 169.55  E-value: 3.75e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   5 LTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSFLD 84
Cdd:pfam00576   1 LTTHVLDTARGRPAAGVRVTLYRLDGDGWTLLAEGTTNADGRCDDLLLEGEALEPGTYRLVFDTGAYFAA--RGVESFYP 78

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1819399233  85 LIPIRFHIgGEGGHYHVPLLLAPGGYSTYRG 115
Cdd:pfam00576  79 EVEVRFGI-TDAEHYHVPLLLSPFGYSTYRG 108
TLP_HIUase cd05822
HIUase (5-hydroxyisourate hydrolase) catalyzes the second step in a three-step ureide pathway ...
 3-116 1.39e-54

HIUase (5-hydroxyisourate hydrolase) catalyzes the second step in a three-step ureide pathway in which 5-hydroxyisourate (HIU), a product of the uricase (urate oxidase) reaction, is hydrolyzed to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU). HIUase has high sequence similarity with transthyretins and is a member of the transthyretin-like protein (TLP) family. HIUase is distinguished from transthyretins by a conserved signature motif at its C-terminus that forms part of the active site. In HIUase, this motif is YRGS, while transthyretins have a conserved TAVV sequence in the same location. Most HIUases are cytosolic but in plants and slime molds, they are peroxisomal based on the presence of N-terminal periplasmic localization sequences. HIUase forms a homotetramer with each subunit consisting of eight beta-strands arranged in two sheets and a short alpha-helix. The central channel of the tetramer contains two independent binding sites, each located between a pair of subunits.


Pssm-ID: 100114  Cd Length: 112  Bit Score: 165.79  E-value: 1.39e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:cd05822     1 GPLSTHVLDTATGKPAAGVAVTLYRLDGNGWTLLATGVTNADGRCDDLLPPGAQLAAGTYKLTFDTGAYFAA--RGQESF 78

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:cd05822    79 YPEVEVRFTITDPTEHYHVPLLLSPFGYSTYRGS 112
hdxy_isourate TIGR02962
hydroxyisourate hydrolase; Members of this family, hydroxyisourate hydrolase, represent a ...
 3-116 3.18e-54

hydroxyisourate hydrolase; Members of this family, hydroxyisourate hydrolase, represent a distinct clade of transthyretin-related proteins. Bacterial members typically are encoded next to ureidoglycolate hydrolase and often near either xanthine dehydrogenase or xanthine/uracil permease genes and have been demonstrated to have hydroxyisourate hydrolase activity. In eukaryotes, a clade separate from the transthyretins (a family of thyroid-hormone binding proteins) has also been shown to have HIU hydrolase activity in urate catabolizing organisms. Transthyretin, then, would appear to be the recently diverged paralog of the more ancient HIUH family. [Purines, pyrimidines, nucleosides, and nucleotides, Other]


Pssm-ID: 274364  Cd Length: 112  Bit Score: 165.03  E-value: 3.18e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:TIGR02962   1 SPLSTHVLDTTSGKPAAGVPVTLYRLDGGGWTPLATGVTNADGRCDGPLPEGEDLAPGIYKLRFDTGDYFAA--RGVESF 78

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:TIGR02962  79 YPEVEVVFTIADPGQHYHVPLLLSPYGYSTYRGS 112
PRK15036 PRK15036
hydroxyisourate hydrolase; Provisional
 5-116 2.99e-29

hydroxyisourate hydrolase; Provisional


Pssm-ID: 184996  Cd Length: 137  Bit Score: 102.37  E-value: 2.99e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   5 LTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEmKPGEYELLFHAGDYFRKKDQgaSSFLD 84
Cdd:PRK15036   29 LSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA-TTGDYRVVFKTGDYFKKQNL--ESFFP 105

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1819399233  85 LIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:PRK15036  106 EIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS 137
TR_THY smart00095
Transthyretin;
5-112 3.29e-03

Transthyretin;


Pssm-ID: 128406  Cd Length: 121  Bit Score: 34.86  E-value: 3.29e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233    5 LTTHVLDLVQGIPASGVFLQL-RRLENGESLLLREAVTNADGRLDApLLEDAEMKPGEYELLFHAGDYFrkKDQGASSFL 83
Cdd:smart00095   6 LMVKVLDAVRGSPAVNVAVKVfKKTEEGTWEPFASGKTNESGEIHE-LTTDEKFVEGLYKVEFDTKSYW--KALGISPFH 82

                           90       100       110
                   ....*....|....*....|....*....|
gi 1819399233   84 DLIPIRFHIGGEGG-HYHVPLLLAPGGYST 112
Cdd:smart00095  83 EYADVVFTANDSGHrHYTIAALLSPYSYST 112
 
Name Accession Description Interval E-value
HiuH COG2351
5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide ...
 3-116 7.03e-57

5-hydroxyisourate hydrolase (purine catabolism), transthyretin-related family [Nucleotide transport and metabolism];


Pssm-ID: 441918  Cd Length: 111  Bit Score: 171.47  E-value: 7.03e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAplLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:COG2351     2 GRLSTHVLDTARGRPAAGVRVELYRLDGDGWTLLAEGVTNADGRIDA--LGGEALAAGTYRLVFDTGDYFAA--RGVPPF 77

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:COG2351    78 LPEVPVRFGIADPEEHYHVPLLLSPWGYSTYRGS 111
Transthyretin pfam00576
HIUase/Transthyretin family; This family includes transthyretin that is a thyroid ...
 5-115 3.75e-56

HIUase/Transthyretin family; This family includes transthyretin that is a thyroid hormone-binding protein that transports thyroxine from the bloodstream to the brain. However, most of the sequences listed in this family do not bind thyroid hormones. They are actually enzymes of the purine catabolism that catalyze the conversion of 5-hydroxyisourate (HIU) to OHCU. HIU hydrolysis is the original function of the family and is conserved from bacteria to mammals; transthyretins arose by gene duplications in the vertebrate lineage. HIUases are distinguished in the alignment from the conserved C-terminal YRGS sequence.


Pssm-ID: 459857  Cd Length: 108  Bit Score: 169.55  E-value: 3.75e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   5 LTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSFLD 84
Cdd:pfam00576   1 LTTHVLDTARGRPAAGVRVTLYRLDGDGWTLLAEGTTNADGRCDDLLLEGEALEPGTYRLVFDTGAYFAA--RGVESFYP 78

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1819399233  85 LIPIRFHIgGEGGHYHVPLLLAPGGYSTYRG 115
Cdd:pfam00576  79 EVEVRFGI-TDAEHYHVPLLLSPFGYSTYRG 108
TLP_HIUase cd05822
HIUase (5-hydroxyisourate hydrolase) catalyzes the second step in a three-step ureide pathway ...
 3-116 1.39e-54

HIUase (5-hydroxyisourate hydrolase) catalyzes the second step in a three-step ureide pathway in which 5-hydroxyisourate (HIU), a product of the uricase (urate oxidase) reaction, is hydrolyzed to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU). HIUase has high sequence similarity with transthyretins and is a member of the transthyretin-like protein (TLP) family. HIUase is distinguished from transthyretins by a conserved signature motif at its C-terminus that forms part of the active site. In HIUase, this motif is YRGS, while transthyretins have a conserved TAVV sequence in the same location. Most HIUases are cytosolic but in plants and slime molds, they are peroxisomal based on the presence of N-terminal periplasmic localization sequences. HIUase forms a homotetramer with each subunit consisting of eight beta-strands arranged in two sheets and a short alpha-helix. The central channel of the tetramer contains two independent binding sites, each located between a pair of subunits.


Pssm-ID: 100114  Cd Length: 112  Bit Score: 165.79  E-value: 1.39e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:cd05822     1 GPLSTHVLDTATGKPAAGVAVTLYRLDGNGWTLLATGVTNADGRCDDLLPPGAQLAAGTYKLTFDTGAYFAA--RGQESF 78

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:cd05822    79 YPEVEVRFTITDPTEHYHVPLLLSPFGYSTYRGS 112
hdxy_isourate TIGR02962
hydroxyisourate hydrolase; Members of this family, hydroxyisourate hydrolase, represent a ...
 3-116 3.18e-54

hydroxyisourate hydrolase; Members of this family, hydroxyisourate hydrolase, represent a distinct clade of transthyretin-related proteins. Bacterial members typically are encoded next to ureidoglycolate hydrolase and often near either xanthine dehydrogenase or xanthine/uracil permease genes and have been demonstrated to have hydroxyisourate hydrolase activity. In eukaryotes, a clade separate from the transthyretins (a family of thyroid-hormone binding proteins) has also been shown to have HIU hydrolase activity in urate catabolizing organisms. Transthyretin, then, would appear to be the recently diverged paralog of the more ancient HIUH family. [Purines, pyrimidines, nucleosides, and nucleotides, Other]


Pssm-ID: 274364  Cd Length: 112  Bit Score: 165.03  E-value: 3.18e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEMKPGEYELLFHAGDYFRKkdQGASSF 82
Cdd:TIGR02962   1 SPLSTHVLDTTSGKPAAGVPVTLYRLDGGGWTPLATGVTNADGRCDGPLPEGEDLAPGIYKLRFDTGDYFAA--RGVESF 78

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1819399233  83 LDLIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:TIGR02962  79 YPEVEVVFTIADPGQHYHVPLLLSPYGYSTYRGS 112
Transthyretin_like cd05469
Transthyretin_like. This domain is present in the transthyretin-like protein (TLP) family ...
 3-116 4.93e-31

Transthyretin_like. This domain is present in the transthyretin-like protein (TLP) family which includes transthyretin (TTR) and a transthyretin-related protein called 5-hydroxyisourate hydrolase (HIUase). TTR and HIUase are homotetrameric proteins with each subunit consisting of eight beta-strands arranged in two sheets and a short alpha-helix. The central channel of the tetramer contains two independent binding sites, each located between a pair of subunits. TTR transports thyroid hormones and retinol in the blood serum of vertebrates while HIUase catalyzes the second step in a three-step ureide pathway. TTRs are highly conserved and found only in vertebrates while the HIUases are found in a wide range of bacterial, plant, fungal, slime mold and vertebrate organisms.


Pssm-ID: 100112  Cd Length: 113  Bit Score: 106.08  E-value: 4.93e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   3 GRLTTHVLDLVQGIPASGVFLQLRRL-ENGESLLLREAVTNADGRLDAPLLEDaEMKPGEYELLFHAGDYFrkKDQGASS 81
Cdd:cd05469     1 CPLMVKVLDAVRGSPAANVAIKVFRKtADGSWEIFATGKTNEDGELHGLITEE-EF*AGVYRVEFDTKSYW--KALGITP 77

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1819399233  82 FLDLIPIRFHIGGEG-GHYHVPLLLAPGGYSTYRGS 116
Cdd:cd05469    78 FHEYAEVVFTANDSGhRHYTIALLLSPFSYSTTAVV 113
PRK15036 PRK15036
hydroxyisourate hydrolase; Provisional
 5-116 2.99e-29

hydroxyisourate hydrolase; Provisional


Pssm-ID: 184996  Cd Length: 137  Bit Score: 102.37  E-value: 2.99e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   5 LTTHVLDLVQGIPASGVFLQLRRLENGESLLLREAVTNADGRLDAPLLEDAEmKPGEYELLFHAGDYFRKKDQgaSSFLD 84
Cdd:PRK15036   29 LSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA-TTGDYRVVFKTGDYFKKQNL--ESFFP 105

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1819399233  85 LIPIRFHIGGEGGHYHVPLLLAPGGYSTYRGS 116
Cdd:PRK15036  106 EIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS 137
TLP_Transthyretin cd05821
Transthyretin (TTR) is a 55 kDa protein responsible for the transport of thyroid hormones and ...
 5-112 2.74e-09

Transthyretin (TTR) is a 55 kDa protein responsible for the transport of thyroid hormones and retinol in vertebrates. TTR distributes the two thyroid hormones T3 (3,5,3'-triiodo-L-thyronine) and T4 (Thyroxin, or 3,5,3',5'-tetraiodo-L-thyronine), as well as retinol (vitamin A) through the formation of a macromolecular complex that includes each of these as well as retinol-binding protein. Misfolded forms of TTR are implicated in the amyloid diseases familial amyloidotic polyneuropathy and senile systemic amyloidosis. TTR forms a homotetramer with each subunit consisting of eight beta-strands arranged in two sheets and a short alpha-helix. The central channel of the tetramer contains two independent binding sites, each located between a pair of subunits, which differ in their ligand binding affinity. A negative cooperativity has been observed for the binding of T4 and other TTR ligands. A fraction of plasma TTR is carried in high density lipoproteins by binding to apolipoprotein AI (apoA-I). TTR is able to proteolytically process apoA-I by cleaving its C-terminus; therefore TTR has protease activity in addition to its function in protein transport.


Pssm-ID: 100113  Cd Length: 121  Bit Score: 51.01  E-value: 2.74e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233   5 LTTHVLDLVQGIPASGVFLQL-RRLENGESLLLREAVTNADGRLDApLLEDAEMKPGEYELLFHAGDYFRKkdQGASSFL 83
Cdd:cd05821     9 LMVKVLDAVRGSPAANVAVKVfKKTADGSWEPFASGKTTETGEIHG-LTTDEQFTEGVYKVEFDTKAYWKK--LGISPFH 85

                          90       100       110
                  ....*....|....*....|....*....|
gi 1819399233  84 DLIPIRFHIGGEGG-HYHVPLLLAPGGYST 112
Cdd:cd05821    86 EYAEVVFTANDSGHrHYTIAALLSPYSYST 115
TR_THY smart00095
Transthyretin;
5-112 3.29e-03

Transthyretin;


Pssm-ID: 128406  Cd Length: 121  Bit Score: 34.86  E-value: 3.29e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1819399233    5 LTTHVLDLVQGIPASGVFLQL-RRLENGESLLLREAVTNADGRLDApLLEDAEMKPGEYELLFHAGDYFrkKDQGASSFL 83
Cdd:smart00095   6 LMVKVLDAVRGSPAVNVAVKVfKKTEEGTWEPFASGKTNESGEIHE-LTTDEKFVEGLYKVEFDTKSYW--KALGISPFH 82

                           90       100       110
                   ....*....|....*....|....*....|
gi 1819399233   84 DLIPIRFHIGGEGG-HYHVPLLLAPGGYST 112
Cdd:smart00095  83 EYADVVFTANDSGHrHYTIAALLSPYSYST 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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