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Conserved domains on  [gi|1828691114|ref|WP_167579098|]
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RepB family plasmid replication initiator protein, partial [Escherichia coli]

Protein Classification

replication initiation protein( domain architecture ID 139766)

replication initiation protein is essential for plasmid DNA replication

CATH:  1.10.10.10
Gene Ontology:  GO:0006270|GO:0003887
SCOP:  4000166

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RPA super family cl19398
Replication initiator protein A; Members of this family of bacterial proteins are ...
 1-55 4.63e-25

Replication initiator protein A; Members of this family of bacterial proteins are single-stranded DNA binding proteins that are involved in DNA replication, repair and recombination.


The actual alignment was detected with superfamily member PRK13742:

Pssm-ID: 473167  Cd Length: 245  Bit Score: 91.94  E-value: 4.63e-25
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1828691114   1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQD 55
Cdd:PRK13742    1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQE 55
 
Name Accession Description Interval E-value
PRK13742 PRK13742
replication initiation protein RepE;
1-55 4.63e-25

replication initiation protein RepE;


Pssm-ID: 184293  Cd Length: 245  Bit Score: 91.94  E-value: 4.63e-25
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1828691114   1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQD 55
Cdd:PRK13742    1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQE 55
Rep_3 pfam01051
Initiator Replication protein; This protein is an initiator of plasmid replication. RepB ...
 14-50 1.13e-05

Initiator Replication protein; This protein is an initiator of plasmid replication. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This family also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene.


Pssm-ID: 460042  Cd Length: 218  Bit Score: 39.96  E-value: 1.13e-05
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1828691114  14 NSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSD 50
Cdd:pfam01051   1 SKELVVKSNDLIEAAYKLTLVELRLFFLLLSKIKDKD 37
 
Name Accession Description Interval E-value
PRK13742 PRK13742
replication initiation protein RepE;
1-55 4.63e-25

replication initiation protein RepE;


Pssm-ID: 184293  Cd Length: 245  Bit Score: 91.94  E-value: 4.63e-25
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1828691114   1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQD 55
Cdd:PRK13742    1 MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQE 55
Rep_3 pfam01051
Initiator Replication protein; This protein is an initiator of plasmid replication. RepB ...
 14-50 1.13e-05

Initiator Replication protein; This protein is an initiator of plasmid replication. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This family also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene.


Pssm-ID: 460042  Cd Length: 218  Bit Score: 39.96  E-value: 1.13e-05
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1828691114  14 NSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSD 50
Cdd:pfam01051   1 SKELVVKSNDLIEAAYKLTLVELRLFFLLLSKIKDKD 37
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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