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Conserved domains on  [gi|1871959880|ref|WP_179988832|]
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MULTISPECIES: thiaminase II [Acinetobacter]

Protein Classification

TenA family protein( domain architecture ID 14443672)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 4-212 9.61e-98

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


:

Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 282.86  E-value: 9.61e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   4 SQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVER 83
Cdd:cd19365     1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  84 TLHDSFMQAFDISkqefESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWVDTY 163
Cdd:cd19365    81 ELHRSFLGEFGID----AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASPNHPYQDWIDTY 156

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1871959880 164 SGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:cd19365   157 SDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
 
Name Accession Description Interval E-value
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 4-212 9.61e-98

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 282.86  E-value: 9.61e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   4 SQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVER 83
Cdd:cd19365     1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  84 TLHDSFMQAFDISkqefESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWVDTY 163
Cdd:cd19365    81 ELHRSFLGEFGID----AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASPNHPYQDWIDTY 156

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1871959880 164 SGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:cd19365   157 SDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-215 4.25e-88

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 259.04  E-value: 4.25e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   1 MRFSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIV 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  81 VERTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVP-NNPYQAW 159
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPpDHPYAEW 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1871959880 160 VDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAYHQ 215
Cdd:COG0819   161 IETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRL 216
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
2-212 4.70e-48

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 164.96  E-value: 4.70e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   2 RFSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVV 81
Cdd:PRK14713  319 PFTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEV 398

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  82 ERTLHDSFMQAFDISKQefeSTPLTLAchhYTSYLLATAWSESYPVVLASLLPCFWIYAEVGK--DIVNNSVPNNPYQAW 159
Cdd:PRK14713  399 ESELHRSWLGDRDADTA---PSPVTLA---YTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAelHARAGNPDDHPYAEW 472

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1871959880 160 VDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:PRK14713  473 LQTYADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQA 525
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 22-213 9.72e-41

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 138.09  E-value: 9.72e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAFDISKQE-F 100
Cdd:TIGR04306  12 PFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAEVLGISDEEyF 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 101 ESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIvnNSVPNNP----YQAWVDTYSGEEFHSAVNNVI 176
Cdd:TIGR04306  92 QKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKL--KGKHTAPehsvYHKWIETYSSSWFREAVNEGE 169

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1871959880 177 QTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:TIGR04306 170 NLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 22-213 2.17e-32

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 116.69  E-value: 2.17e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAFDISKQEFE 101
Cdd:pfam03070  15 PFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAEALGLSREDLS 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 102 STPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVN--NSVPNNPYQAWVDTYSGEEFHSAVNNVIQTI 179
Cdd:pfam03070  95 AYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEkiRALEGPEYYEWVKTYASEYFRAAVEEAKRLL 174

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1871959880 180 DRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:pfam03070 175 DRLLAYVSDEQLEELQEIFLKALEFEYSFWTMAL 208
 
Name Accession Description Interval E-value
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 4-212 9.61e-98

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 282.86  E-value: 9.61e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   4 SQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVER 83
Cdd:cd19365     1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  84 TLHDSFMQAFDISkqefESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWVDTY 163
Cdd:cd19365    81 ELHRSFLGEFGID----AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASPNHPYQDWIDTY 156

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1871959880 164 SGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:cd19365   157 SDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-215 4.25e-88

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 259.04  E-value: 4.25e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   1 MRFSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIV 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  81 VERTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVP-NNPYQAW 159
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPpDHPYAEW 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1871959880 160 VDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAYHQ 215
Cdd:COG0819   161 IETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRL 216
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 3-213 1.53e-63

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 196.62  E-value: 1.53e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVE 82
Cdd:cd19366     1 FSERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  83 RTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIV--NNSVPNNPYQAWV 160
Cdd:cd19366    81 MDLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAeqGGALEHNPYREWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1871959880 161 DTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:cd19366   161 EMYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 3-213 1.39e-57

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 181.23  E-value: 1.39e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVE 82
Cdd:cd19364     1 FTEELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  83 RTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWVDT 162
Cdd:cd19364    81 LALRETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADAGSPVPLYQRWIDT 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1871959880 163 YSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:cd19364   161 YASDEFTESVQQQIDLVDRLAEEASEEEREKMKQAFLISSYYELQFWEMAY 211
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 14-210 1.18e-54

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 173.30  E-value: 1.18e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  14 LYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIaIVVERTLHDSFMQAF 93
Cdd:cd16099     1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFLAELINV-LDDELELHEKLLAEL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  94 DISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWVDTYSGEEFHSAVN 173
Cdd:cd16099    80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASLPEHPPYRFWIDFYASDEYQELVE 159

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1871959880 174 NVIQTIDRIAARCDADTlEKMHHAYTKAAELEWLFWD 210
Cdd:cd16099   160 ELLQLLDQLAAAGEEEK-EELKEIFLTSLRYELMFWD 195
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
2-212 4.70e-48

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 164.96  E-value: 4.70e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   2 RFSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVV 81
Cdd:PRK14713  319 PFTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEV 398

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  82 ERTLHDSFMQAFDISKQefeSTPLTLAchhYTSYLLATAWSESYPVVLASLLPCFWIYAEVGK--DIVNNSVPNNPYQAW 159
Cdd:PRK14713  399 ESELHRSWLGDRDADTA---PSPVTLA---YTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAelHARAGNPDDHPYAEW 472

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1871959880 160 VDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:PRK14713  473 LQTYADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQA 525
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 22-213 5.93e-46

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 151.13  E-value: 5.93e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKiAIVVERTLHDSFMQAFDISKQEFE 101
Cdd:cd19367    10 PFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILL-AILEEMELHVKYCAEWGISEEELE 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 102 STPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVG---KDIVNNSVPNNPYQAWVDTYSGEEFHSAVNNVIQT 178
Cdd:cd19367    89 ATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGlrlAADPATKLEGNPYWSWIETYASDEYQEAVREGIEL 168

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1871959880 179 IDRIAARCDADT-LEKMHHAYTKAAELEWLFWDSAY 213
Cdd:cd19367   169 LEKLAAERPSPArLEELVKIFATATRLEIGFWDMGL 204
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 22-213 9.72e-41

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 138.09  E-value: 9.72e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAFDISKQE-F 100
Cdd:TIGR04306  12 PFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAEVLGISDEEyF 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 101 ESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIvnNSVPNNP----YQAWVDTYSGEEFHSAVNNVI 176
Cdd:TIGR04306  92 QKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKL--KGKHTAPehsvYHKWIETYSSSWFREAVNEGE 169

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1871959880 177 QTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:TIGR04306 170 NLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 22-210 1.22e-40

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 138.10  E-value: 1.22e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAFDISKQEFE 101
Cdd:cd19361    20 PFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEEMDIHRGYMKRLGITEEEIE 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 102 STPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIV--NNSVPNNP-YQAWVDTYSGEEFHSAVNNVIQT 178
Cdd:cd19361   100 NTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVerYPAALEHEfYGEWVKGYSSEEYAEANQELIDL 179

                         170       180       190
                  ....*....|....*....|....*....|..
gi 1871959880 179 IDRIAARCDADTLEKMHHAYTKAAELEWLFWD 210
Cdd:cd19361   180 LDRLTEDISEEQIEKLEEIFVNCSRYELKFWD 211
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 3-212 2.98e-40

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 146.27  E-value: 2.98e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVE 82
Cdd:PRK09517  546 FTRALWEASGDIIAEINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAECIVVE 625

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  83 RTLHDSFMqafdiSKQEFES--TPLTLAchhYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWV 160
Cdd:PRK09517  626 AELHRSYL-----SGKEAPSapSPVTMA---YTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWL 697

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1871959880 161 DTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:PRK09517  698 NTYSGEEFIAGTRAAIARVEKALENAGPEQRVDAARAFLSASVHEREFFDQA 749
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 3-205 4.73e-40

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 136.22  E-value: 4.73e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKiAIVVE 82
Cdd:cd19368     1 LAKRLWKKNRDEALFALYHPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRK-GVLEE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  83 RTLHDSFMQAFDI-SKQEFESTPLTLAchhYTSYLLATAWSESYPVV--LASLLPCFWIYAEVGKDI--VNNSVPNNPYQ 157
Cdd:cd19368    80 LKLHDSYAEEWGVdLPKEVTPDPATRK---YTDFLLATASGKVKVAAytLAAMAPCMRLYAFLGQELarALDDTEDHPYK 156

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1871959880 158 AWVDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELE 205
Cdd:cd19368   157 KWIDTYSSQEFEALALQLEDLLDKLSASLTGEELEALEKLYRRAMKLE 204
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 3-210 6.70e-39

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 133.48  E-value: 6.70e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVE 82
Cdd:cd19360     1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  83 RTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIV--NNSVPNNPYQAWV 160
Cdd:cd19360    81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIeeGKPTPDNPFYEWI 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1871959880 161 DTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWD 210
Cdd:cd19360   161 DFYADDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWE 210
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 14-210 5.92e-35

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 123.11  E-value: 5.92e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  14 LYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAF 93
Cdd:cd19369     2 IWEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKEF 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  94 DISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVP---NNPYQAWVDTYSGEEFHS 170
Cdd:cd19369    82 GLTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDnleSNPYKDWIEDYASEEYAE 161

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 1871959880 171 AVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWD 210
Cdd:cd19369   162 YCKEWIDFADRLCENLSEEEKEKLKEIFRKASLYELKFWD 201
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 4-219 6.06e-33

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 123.92  E-value: 6.06e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   4 SQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVER 83
Cdd:PTZ00347   14 SEALWKENQDLAMMSLHLPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELK 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  84 TLHDSFMQAFDISKQEfestpltLACHHYTSYLLATAWSESYP--VVLASLLPCFWIYAEVGKDIVNNSV--PNNPYQAW 159
Cdd:PTZ00347   94 NCHHHYIDNPDAAGPE-------AACRKYVDFLLASGNADTLGpsVVIAAVIPCARLYAWVGQELTNEVEltESHPFRRW 166

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 160 VDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKmhhAYTKAAELEWLFWDSAYHQRQWP 219
Cdd:PTZ00347  167 LLSYSDEPINTSVEQLESLLDKYIRPGEFSEVAQ---AYRRAMELEYDFFDSFGYCLGRP 223
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 22-213 2.17e-32

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 116.69  E-value: 2.17e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  22 PFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDSFMQAFDISKQEFE 101
Cdd:pfam03070  15 PFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAEALGLSREDLS 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880 102 STPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVN--NSVPNNPYQAWVDTYSGEEFHSAVNNVIQTI 179
Cdd:pfam03070  95 AYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEkiRALEGPEYYEWVKTYASEYFRAAVEEAKRLL 174

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1871959880 180 DRIAARCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:pfam03070 175 DRLLAYVSDEQLEELQEIFLKALEFEYSFWTMAL 208
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 9-213 1.65e-30

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 111.65  E-value: 1.65e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   9 QRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAKIAIVVERTLHDS 88
Cdd:cd19363     7 RDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEFANYEE 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  89 FMQAFDIS---KQEFESTPLTLAchhYTSYLLATAWSESYPVVLASLLPCFWIYAEV----GKDIVNNsvPNNPYQAWVD 161
Cdd:cd19363    87 LLDELGLSledAIKVEPFPTNVA---YMNFLLSTSSLGSFYEGLAALLPCFWSYLEIaeyhKDKLSEN--PNDIYRDWAS 161

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1871959880 162 TYSGEEFHSAVNNVIQTIDRIaarCDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:cd19363   162 VYLSKEYKELVERLRRIVDKY---GEGEPFEKLKRIFKTASKYEYMFWDAAY 210
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 3-213 1.07e-24

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 96.48  E-value: 1.07e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   3 FSQEAWQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFseAAKIAIVV- 81
Cdd:cd19358     1 FSDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRL--ARFLGFLAn 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  82 -ERTLHDSFMQAFDISKQEFESTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNSVPNNPYQAWV 160
Cdd:cd19358    79 dENDYFERAFAALGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRAAAAAPLRFKHQEWI 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1871959880 161 DTYSGEEFHSAVNNVIQTIDRIAArcDADTLEKMHHAYTKAAELEWLFWDSAY 213
Cdd:cd19358   159 DLHSGPEFEAWVDFLRDEVDRVGP--TEEERERLEAVFARAVELEIAFFDAAY 209
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 23-212 2.00e-21

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 87.88  E-value: 2.00e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  23 FNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAfdaddiiQFSEAAKIAIVVERT------LHDSFMQAFDIS 96
Cdd:cd19362    20 FVERMRDGTLPLDNFRYYLIQDSKYVEEMLRALLRASSKA-------PLDKAIKILNSVFSGrdkgmeVHKFLYSELGIT 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  97 KQEFESTPLTLACHHYTSYLLATAwSESYPVVLASLLPCFWIYAEVGKDIVNNsvPNNPYQAWVDTYSGEEFHSAVNNVI 176
Cdd:cd19362    93 EDEIRRTGYNLVNYAYTRHLYYYS-TLGWPQFLAAWAPCMWGYSEIGKYVLNS--PNELYKTWASFYASKDYKKRVEAIL 169

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1871959880 177 QTIDRIaarcdaDTLEKMHHAYTKAAELEWLFWDSA 212
Cdd:cd19362   170 EALDSI------EDTEDIKNIFRNSVNFEIMFWDAA 199
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 8-211 2.67e-20

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 85.09  E-value: 2.67e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880   8 WQRNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAFDADDIIQFSEAAK----IAIVVER 83
Cdd:cd19359     5 WNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLLAFLAAKIKsyldYAEDFLK 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  84 TLHDSFMQAFDISKqefesTPLTLACHHYTSYLLATAWSESYPVVLASLLPCFWIYAEVGKDIVNNS--VPNNPYQAWVD 161
Cdd:cd19359    85 TCHIKLGIPDVVDG-----VKPSPALKAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNEDPsdKNTNFYKTWIE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1871959880 162 TYSGEEfhSAVNNVIQTIDRIAARCDADtLEKMHHAYTKAAELEWLFWDS 211
Cdd:cd19359   160 PNLPDP--SSAKQLEFFLNANAAWSKID-REKANEIFRQAMQLEINFFNS 206
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 10-211 1.89e-12

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 63.88  E-value: 1.89e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  10 RNLALYQKTLALPFNQELAQGTLSKAAFCHYVIQDSHYLIAYGRALAVCAAKAfDADDIIQFSEAAKI---AIV-VERTL 85
Cdd:cd19357     7 LHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLLARA-PLPSSSLNQRLLDVllgALAnLRREL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1871959880  86 hdSFMQA------FDISKQEFESTPltlACHHYTSYLLATA-WSESYPVVLAsllpCFW----IYAE----VGKDIVNNS 150
Cdd:cd19357    86 --AFFEEtaaeygLDLPGLGVPPSP---ATRAYVDFLASLAsEGVSYLEGLV----VLWatekVYLDawsyARSFLPSDA 156

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1871959880 151 VPNNPYQAWVDTYSGEEFHSAVNNVIQTIDRIAARCDADTLEKMHHAYTKAAELEWLFWDS 211
Cdd:cd19357   157 DGGALYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVLERAEEVWRRVLELEEAFWPM 217
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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