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Conserved domains on  [gi|1897086191|ref|WP_187003367|]
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DUF4297 domain-containing protein [Vibrio metschnikovii]

Protein Classification

DUF4297 domain-containing protein( domain architecture ID 10624361)

DUF4297 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Cap4_nuclease pfam14130
Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase ...
 11-217 2.76e-47

Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase associated protein 4 (Cap4) from bacteria and archaea. CD-NTase-associated protein 4 (Cap4) is a nucleotide second messenger sensor that functions is CBASS immunity. This is the effector domain which has dsDNA nuclease activity, sharing structural homology with type II restriction endonucleases and it has the putative active-site conserved residues required for divalent metal coordination. This domain is inactive as a monomer; after Cap4 signal recognition through its C-terminal SAVED domain, it oligomerises to closely locate two nuclease effector domains. This domain does not participates in ligand specificity and it has a promiscuous DNA cleavage response.


:

Pssm-ID: 433740  Cd Length: 212  Bit Score: 159.48  E-value: 2.76e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  11 RESAGASTFGKYNFQFHWALCEIIEKHKNKKEYALLIEYHEDVVIADSlDADEAQFEFYQVK-NQTVKFTPASLTNRKKG 89
Cdd:pfam14130   1 RESGGSETKKGFRYQYHWALCKLLDLHKEKDDYALICEYHEDIVILDS-DHSPGKFEFYQVKtKESGKWTIKDLTKSKKG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  90 ANdTLKNSVLGKLLSSciNTEYEDRITTIGLVSSSGFSLNL-DKGLKLDVI-KVGDIAASDLASLTQDINNELGIK---V 164
Cdd:pfam14130  80 EK-KKKSSILGKLLKS--DLDFPDRIAKLAFVSNAPFSLTLkDGKISLSLRyCLSELSQELIKKLEKSLKDELGLEktdV 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1897086191 165 LP-EHLQFIVPDIQLKNQEDYVLSHFARLVNALFPGAFFNPVNIYRSIVDEMGR 217
Cdd:pfam14130 157 IDlNNLKFIASSLSLEDLEIHLIGKIAQLLEEVFPNEDLNANAIYRLLIDELRR 210
 
Name Accession Description Interval E-value
Cap4_nuclease pfam14130
Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase ...
 11-217 2.76e-47

Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase associated protein 4 (Cap4) from bacteria and archaea. CD-NTase-associated protein 4 (Cap4) is a nucleotide second messenger sensor that functions is CBASS immunity. This is the effector domain which has dsDNA nuclease activity, sharing structural homology with type II restriction endonucleases and it has the putative active-site conserved residues required for divalent metal coordination. This domain is inactive as a monomer; after Cap4 signal recognition through its C-terminal SAVED domain, it oligomerises to closely locate two nuclease effector domains. This domain does not participates in ligand specificity and it has a promiscuous DNA cleavage response.


Pssm-ID: 433740  Cd Length: 212  Bit Score: 159.48  E-value: 2.76e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  11 RESAGASTFGKYNFQFHWALCEIIEKHKNKKEYALLIEYHEDVVIADSlDADEAQFEFYQVK-NQTVKFTPASLTNRKKG 89
Cdd:pfam14130   1 RESGGSETKKGFRYQYHWALCKLLDLHKEKDDYALICEYHEDIVILDS-DHSPGKFEFYQVKtKESGKWTIKDLTKSKKG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  90 ANdTLKNSVLGKLLSSciNTEYEDRITTIGLVSSSGFSLNL-DKGLKLDVI-KVGDIAASDLASLTQDINNELGIK---V 164
Cdd:pfam14130  80 EK-KKKSSILGKLLKS--DLDFPDRIAKLAFVSNAPFSLTLkDGKISLSLRyCLSELSQELIKKLEKSLKDELGLEktdV 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1897086191 165 LP-EHLQFIVPDIQLKNQEDYVLSHFARLVNALFPGAFFNPVNIYRSIVDEMGR 217
Cdd:pfam14130 157 IDlNNLKFIASSLSLEDLEIHLIGKIAQLLEEVFPNEDLNANAIYRLLIDELRR 210
 
Name Accession Description Interval E-value
Cap4_nuclease pfam14130
Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase ...
 11-217 2.76e-47

Cap4 dsDNA endonuclease; This domain family corresponds to the N-terminal of CD-NTase associated protein 4 (Cap4) from bacteria and archaea. CD-NTase-associated protein 4 (Cap4) is a nucleotide second messenger sensor that functions is CBASS immunity. This is the effector domain which has dsDNA nuclease activity, sharing structural homology with type II restriction endonucleases and it has the putative active-site conserved residues required for divalent metal coordination. This domain is inactive as a monomer; after Cap4 signal recognition through its C-terminal SAVED domain, it oligomerises to closely locate two nuclease effector domains. This domain does not participates in ligand specificity and it has a promiscuous DNA cleavage response.


Pssm-ID: 433740  Cd Length: 212  Bit Score: 159.48  E-value: 2.76e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  11 RESAGASTFGKYNFQFHWALCEIIEKHKNKKEYALLIEYHEDVVIADSlDADEAQFEFYQVK-NQTVKFTPASLTNRKKG 89
Cdd:pfam14130   1 RESGGSETKKGFRYQYHWALCKLLDLHKEKDDYALICEYHEDIVILDS-DHSPGKFEFYQVKtKESGKWTIKDLTKSKKG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1897086191  90 ANdTLKNSVLGKLLSSciNTEYEDRITTIGLVSSSGFSLNL-DKGLKLDVI-KVGDIAASDLASLTQDINNELGIK---V 164
Cdd:pfam14130  80 EK-KKKSSILGKLLKS--DLDFPDRIAKLAFVSNAPFSLTLkDGKISLSLRyCLSELSQELIKKLEKSLKDELGLEktdV 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1897086191 165 LP-EHLQFIVPDIQLKNQEDYVLSHFARLVNALFPGAFFNPVNIYRSIVDEMGR 217
Cdd:pfam14130 157 IDlNNLKFIASSLSLEDLEIHLIGKIAQLLEEVFPNEDLNANAIYRLLIDELRR 210
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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