|
Name |
Accession |
Description |
Interval |
E-value |
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
28-362 |
1.04e-146 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 419.93 E-value: 1.04e-146
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 28 LMDLDAFFASVEMLDHPEWRGKPLIVGGDADsRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQ 107
Cdd:COG0389 5 HVDMDAFYASVEQRDRPELRGKPVAVGGDNN-RGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYRDVSRR 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 108 VMAILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRVAA-LGVTCSIGVGCNKTVAKIASERDKPFGLTIVR 186
Cdd:COG0389 84 VMAILERYTPLVEPLSIDEAFLDVTGSARLFGSAEAIARRIRRRIRReTGLTVSVGIAPNKFLAKIASDLAKPDGLTVIP 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 187 PGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVtslDEEREVKS 266
Cdd:COG0389 164 PGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPV---EPRRPRKS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 267 VSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLDN 346
Cdd:COG0389 241 IGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTTRSRTLPEPTDDTAELLRAARELLER 320
|
330
....*....|....*.
gi 1952248250 347 IWQEGMHVRLAGIGMS 362
Cdd:COG0389 321 IYRPGRPVRLLGVRLS 336
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
29-364 |
1.23e-146 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 419.62 E-value: 1.23e-146
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 29 MDLDAFFASVEMLDHPEWRGKPLIVGGDADsRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQV 108
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAVGGSSD-RGVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRVAA-LGVTCSIGVGCNKTVAKIASERDKPFGLTIVRP 187
Cdd:cd03586 82 MEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATEIAKEIRARIREeTGLTASAGIAPNKFLAKIASDLNKPNGLTVIPP 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 188 GTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVtslDEEREVKSV 267
Cdd:cd03586 162 EDVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPV---EPDRERKSI 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 268 SNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLDNI 347
Cdd:cd03586 239 GVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLEEL 318
|
330
....*....|....*..
gi 1952248250 348 WQeGMHVRLAGIGMSDF 364
Cdd:cd03586 319 LD-GRPIRLLGVRLSGL 334
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
32-375 |
8.16e-115 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 339.02 E-value: 8.16e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 32 DAFFASVEMLDHPEWRGKPLIVGGDADSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQVMAI 111
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVGGSPGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIREI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 112 LADETPRVERVSIDEAFIDITpgrfapEDPL------LVARRISDRVAA-LGVTCSIGVGCNKTVAKIASERDKPFGLTI 184
Cdd:PRK02406 82 FRRYTDLIEPLSLDEAYLDVT------DNKLcigsatLIAQEIRQDIFEeLGLTASAGVAPNKFLAKIASDWNKPNGLFV 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 185 VRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVTSldeEREV 264
Cdd:PRK02406 156 ITPEEVDAFLATLPVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHFGKFGRRLYERARGIDERPVKP---DRER 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 265 KSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQG--LTGATVTLKLKYSYGSgRTAQRRLPHPTDDENIFVAVALE 342
Cdd:PRK02406 233 KSVGVERTFAEDLYDLEACLAELPRLAEKLERRLERAKpdKRIKTVGVKLKFADFQ-QTTKEHTADPLDKADLIELLAQA 311
|
330 340 350
....*....|....*....|....*....|...
gi 1952248250 343 LLDNiwQEGMHVRLAGIGMSDFNHQGGIQTDLF 375
Cdd:PRK02406 312 LLRR--LGGRGVRLLGVGVTLLEPQLERQLLLD 342
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
29-411 |
4.98e-97 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 296.46 E-value: 4.98e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 29 MDLDAFFASVEMLDHPEWRGKPLIVGGDadSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQV 108
Cdd:PRK02794 41 IDCDAFYASVEKRDNPELRDKPVIIGGG--KRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKPDMEKYVRVGREV 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETPRVERVSIDEAFIDITPG-RFAPEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIASERDKPFGLTIVR 186
Cdd:PRK02794 119 RAMMQALTPLVEPLSIDEAFLDLSGTeRLHGAPPAVVLARFARRVeREIGITVSVGLSYNKFLAKIASDLDKPRGFSVIG 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 187 PGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVtslDEEREVKS 266
Cdd:PRK02794 199 RAEALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRLARGIDDRKV---SPDREAKS 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 267 VSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLDn 346
Cdd:PRK02794 276 VSAETTFETDLSDFEDLEPILWRLSEKVSRRLKAAGLAGRTVTLKLKTADFRLRTRRRTLEDPTQLADRIFRTARELLE- 354
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1952248250 347 iwQE--GMHVRLAGIGMSDFNHQGGIQ-TDLFcevDGRGAQSSDRRDlsvAIDAVRDKFGDTALSFGR 411
Cdd:PRK02794 355 --KEtdGTAFRLIGIGVSDLSPADEADpPDLL---DPQATRRAAAER---AIDALRAKFGAAAVETGR 414
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
29-411 |
1.23e-96 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 296.46 E-value: 1.23e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 29 MDLDAFFASVEMLDHPEWRGKPLIVGGdADSRGVVSTCSYEARRFGVHSAMASAEARRLCPQ-AIWTHGHFDRYREVSAQ 107
Cdd:PRK03348 10 LDMDAFFASVEQLTRPTLRGRPVLVGG-LGGRGVVAGASYEARVFGARSAMPMHQARRLVGNgAVVLPPRFVVYRAASRR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 108 VMAILADETPRVERVSIDEAFIDITPGRFA-PEDPLLVARRISDRVAAL-GVTCSIGVGCNKTVAKIASERDKPFGLTIV 185
Cdd:PRK03348 89 VFDTLRELSPVVEQLSFDEAFVEPAELAGAsAEEVEAFAERLRARVREEtGLPASVGAGSGKQIAKIASGLAKPDGIRVV 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 186 RPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFG-VNGERMRQRALGLEVSEVTsldEEREV 264
Cdd:PRK03348 169 PPGEERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSEAEVANLLGaTVGPALHRLARGIDDRPVA---ERAEA 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 265 KSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELL 344
Cdd:PRK03348 246 KQISAESTFAVDLTTRAQLREAIERIAEHAHRRLLKDGRGARTVTVKLRKSDFSTLTRSATLPYATDDAAVLAATARRLL 325
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1952248250 345 DNIWQEGmHVRLAGIGMSDFNHQggIQTDLFCEVDgRGAQSSDRRDLSVAIDAVRDKFGDTALSFGR 411
Cdd:PRK03348 326 LDPDEIG-PIRLVGVGFSGLSDV--RQESLFPELD-LARETAPAEEVETESAAGPSDSPAAARVEAP 388
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
30-414 |
5.66e-95 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 290.78 E-value: 5.66e-95
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVGGDA-DSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQV 108
Cdd:PRK01810 11 DMNSFFASVEIAYDPSLQGKPLAVAGNEkERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRRPNFDRYREASRQM 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETPRVERVSIDEAFIDITPgRFAPEDPLLVARRISDR-VAALGVTCSIGVGCNKTVAKIASERDKPFGLTIVRP 187
Cdd:PRK01810 91 FQILSEFTPLVQPVSIDEGYLDITD-CYALGSPLEIAKMIQQRlLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRK 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 188 GTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVtslDEER--EVK 265
Cdd:PRK01810 170 RDVPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGIDDRPV---DPEAiyQFK 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 266 SVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLD 345
Cdd:PRK01810 247 SVGNSTTLSHDMDEEKELLDVLRRLSKSVSKRLQKKTVVSYNVQIMIRYHDRRTITRSKTLKNPIWEKRDIFQAASRLFK 326
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1952248250 346 NIWqEGMHVRLAGIGMSD-FNHQGGI-QTDLFcevdgRGAQSSDRRDLSVAIDAVRDKFGDTALSFGRQSR 414
Cdd:PRK01810 327 QHW-NGDPVRLLGVTATDlEWKTEAVkQLDLF-----SFEEDAKEEPLLAVIDQINDKYGMPLLQRGSQLL 391
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
30-364 |
2.42e-93 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 284.69 E-value: 2.42e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVGGDADsRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQVM 109
Cdd:PRK14133 9 DMDAFFASVEQMDNPKLKGKPVIVGGISE-RGVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVRHERYKEVSKNIF 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 110 AILADETPRVERVSIDEAFIDITPGRfapEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIASERDKPFGLTIVRPG 188
Cdd:PRK14133 88 KILYEVTPIVEPVSIDEAYLDITNIK---EEPIKIAKYIKKKVkKETGLTLSVGISYNKFLAKLASDWNKPDGIKIITED 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 189 TEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVTSldeEREVKSVS 268
Cdd:PRK14133 165 MIPDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYFGKFGVEIYERIRGIDYREVEV---SRERKSIG 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 269 NERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLDNiW 348
Cdd:PRK14133 242 KETTLKKDTKDKEELKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQTHTKSKTLNDYIRDKEEIYNVACEILEH-I 320
|
330
....*....|....*.
gi 1952248250 349 QEGMHVRLAGIGMSDF 364
Cdd:PRK14133 321 NIKEPIRLIGLSVSNL 336
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
25-410 |
1.09e-86 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 269.57 E-value: 1.09e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 25 AVGLMDLDAFFASVEMLDHPEWRGKPLIVGGDADSR-GVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYRE 103
Cdd:PRK03103 4 VILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRsGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQRYID 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 104 VSAQVMAILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIASE---RDKP 179
Cdd:PRK03103 84 VSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFGSPLEIAQKIQQRImRETGVYARVGIGPNKLLAKMACDnfaKKNP 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 180 FGLTIVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVT--S 257
Cdd:PRK03103 164 DGLFTLDKEDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIDYSPVTphS 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 258 LDEErevKSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLK---YSYGSGRTAQRRLPHPTDDEN 334
Cdd:PRK03103 244 LDRQ---KAIGHQMTLPRDYRGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRgadFDWPTGFSRQMTLPEPTNLAM 320
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1952248250 335 IFVAVALELLDNIWQeGMHVRLAGIGMSDFNHQGGIQTDLFCEVDgrgaqssDRRDLSVAIDAVRDKFGDTALSFG 410
Cdd:PRK03103 321 EVYEAACKLFHRHWD-GKPVRRVGVTLSNLVSDDVWQLSLFGDRE-------RKRSLGYVMDDIKNRFGPTAILRA 388
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
30-410 |
3.79e-83 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 259.92 E-value: 3.79e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVGGdadsrGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQVM 109
Cdd:PRK03858 10 DLDSFYASVEQRDDPALRGRPVIVGG-----GVVLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPRMSAYSRASKAVF 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 110 AILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRVAA-LGVTCSIGVGCNKTVAKIASERDKPFGLTIVRPG 188
Cdd:PRK03858 85 EVFRDTTPLVEGLSIDEAFLDVGGLRRISGTPVQIAARLRRRVREeVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPD 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 189 TEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGvngermrqRALGLEVSEVTSLDEEREV---- 264
Cdd:PRK03858 165 RELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELPESALVSLLG--------PAAGRHLHALAHNRDPRRVetgr 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 265 --KSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALE 342
Cdd:PRK03858 237 rrRSVGAQRALGRGPNSPAEVDAVVVALVDRVARRMRAAGRTGRTVVLRLRFDDFTRATRSHTLPRPTASTATLLAAARD 316
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1952248250 343 LLDN----IWQEGmhVRLAGIGMSDFNHQGGIQTDLFCEVDGRGAqssdrrDLSVAIDAVRDKFGDTALSFG 410
Cdd:PRK03858 317 LVAAaaplIAERG--LTLVGFAVSNLDDDGAQQLELPFGLRRPGS------ALDAALDAVRDRFGNAAVTRA 380
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
29-363 |
2.06e-73 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 233.02 E-value: 2.06e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 29 MDLDAFFASVEMLDHPEWRGKPLIVGGDADSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQV 108
Cdd:cd00424 3 IDFDNFFASVEQLARPELKGRPVVVVPFNSDSTCVIACSYEARKYGVKRGMPVREARKMCPNLILVPARLDLYRRLSERL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRVAA--LGVTCSIGVGCNKTVAKIASERDKPFGLTIVR 186
Cdd:cd00424 83 LSELEEVAPLVEVASIDELFLDLTGSARLLGLGSEVALRIKRHIAEqlGGITASIGIASNKLLAKLAAKYAKPDGLTILD 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 187 PGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRA-PESTLASIFGVNGERMRQRALGLEVSEVTsldEEREVK 265
Cdd:cd00424 163 PEDLPGFLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAAsPDALLALWGGVSGERLWYALRGIDDEPLS---PPRPRK 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 266 SVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGR------TAQRRLPHPTDDENIFvAV 339
Cdd:cd00424 240 SFSHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDGRGATRLRLWLRTVDGRWSghadipSRSAPRPISTEDGELL-HA 318
|
330 340
....*....|....*....|....
gi 1952248250 340 ALELLDNIWQEGMHVRLAGIGMSD 363
Cdd:cd00424 319 LDKLWRALLDDKGPRRLRRLGVRL 342
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
27-363 |
5.11e-67 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 216.65 E-value: 5.11e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 27 GLMDLDAFFASVEMLDHPEWRGKPLIVGGDADSRGVVStcSYEARRFGVHSAMASAEARRLCPQ---AIWThGHFDRYRE 103
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGCVIAR--SPEAKALGIKMGSPYFKVPDLLERhgvAVFS-SNYALYGD 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 104 VSAQVMAILADETPRVERVSIDEAFIDITpGRFAPEDPLLVARRISDRVAA-LGVTCSIGVGCNKTVAKIASE-----RD 177
Cdd:cd01700 78 MSRRIMSILERFSPDVEVYSIDESFLDLT-GSLRFGDLEELARKIRRRILQeTGIPVTVGIGPTKTLAKLANDlakkkNP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 178 KPFGLTIVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGLEVSEVTS 257
Cdd:cd01700 157 YGGVVDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 258 LDEERevKSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYS-YGSGRT---AQRRLPHPTDDE 333
Cdd:cd01700 237 YPPPK--KSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSgFSRQPKyysATNTLPYPTNDT 314
|
330 340 350
....*....|....*....|....*....|
gi 1952248250 334 NIFVAVALELLDNIWQEGMHVRLAGIGMSD 363
Cdd:cd01700 315 REIVKAALRLLYAIYRPGYAYRKAGVMLSD 344
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
30-359 |
9.52e-66 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 213.35 E-value: 9.52e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVGGDAD---SRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSA 106
Cdd:PRK03352 11 DLDQFIAAVELLRRPELAGLPVIVGGNGDptePRKVVTCASYEARAFGVRAGMPLRTAARRCPDAVFLPSDPAAYDAASE 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 107 QVMAILADETPRVERVSIDEAFIDITPgrfapEDPLLVARRISDRVA-ALGVTCSIGVGCNKTVAKIASERDKPFGLTIV 185
Cdd:PRK03352 91 EVMATLRDLGVPVEVWGWDEAFLGVDT-----DDPEALAEEIRAAVLeRTGLSCSVGIGDNKLRAKIATGFAKPAGVFRL 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 186 RPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFG-VNGERMRQRALGLEVSEVTslDEEREV 264
Cdd:PRK03352 166 TDANWMAVMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGpTTGPWLLLLARGGGDTEVS--AEPWVP 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 265 KSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELL 344
Cdd:PRK03352 244 RSRSREVTFPQDLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTATFYTRTKIRKLPEPTTDPDVIEAAALDVL 323
|
330
....*....|....*
gi 1952248250 345 DNIwQEGMHVRLAGI 359
Cdd:PRK03352 324 DRF-ELDRPVRLLGV 337
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
30-175 |
1.87e-55 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 180.08 E-value: 1.87e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVGGDaDSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQVM 109
Cdd:pfam00817 2 DMDAFFASVELLRDPELKGKPVAVGGG-NGRGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRKIF 80
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1952248250 110 AILAD-ETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRVAA-LGVTCSIGVGCNKTVAKIASE 175
Cdd:pfam00817 81 EILRRfSTPKVEQASIDEAFLDLTGLEKLFGAEEALAKRLRREIAEeTGLTCSIGIAPNKLLAKLASD 148
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
30-344 |
1.60e-51 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 177.89 E-value: 1.60e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIV----GGDADsrgvVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVS 105
Cdd:cd01701 53 DFDCFFVSVSIRNRPDLKGKPVAVchgkGPNSE----IASCNYEARSYGIKNGMWVGQAKKLCPQLVTLPYDFEAYEEVS 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 106 AQVMAILADETPRVERVSIDEAFIDIT-PGRFAPEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIASERDKPFGLT 183
Cdd:cd01701 129 LTFYEILASYTDNIEAVSCDEALIDITsLLEETYELPEELAEAIRNEIrETTGCSASVGIGPNILLARLATRKAKPDGQY 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 184 IVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLG--ELSRAPESTLASIFGV-NGERMRQRALGLEVSEVTsldE 260
Cdd:cd01701 209 HLSAEKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGDTCGglELRSKTKEKLQKVLGPkTGEKLYDYCRGIDDRPVT---G 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 261 EREVKSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKL------------KYSyGSG------RTA 322
Cdd:cd01701 286 EKERKSVSAEINYGIRFTNVDDVEQFLQRLSEELSKRLEESNVTGRQITLKLmkrapgapieppKYM-GHGicdsfsKSS 364
|
330 340
....*....|....*....|..
gi 1952248250 323 qrRLPHPTDDENIFVAVALELL 344
Cdd:cd01701 365 --TLGVATDDSGVIGTEAKKLF 384
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
28-237 |
5.66e-42 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 151.10 E-value: 5.66e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 28 LMDLDAFFASVEMLDHPEWRGKPLIV---GGDADSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREV 104
Cdd:PRK01216 5 FVDFDYFFAQVEEVLNPSLKGKPVVVcvySGRFEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEVYQQV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 105 SAQVMAILADETPRVERVSIDEAFIDITPGRFAPEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIASERDKPFGLT 183
Cdd:PRK01216 85 SNRIMKLLREYSEKIEIASIDEAYLDISDKVKNYQDAYNLGLEIKNKIlEKEKITVTVGISKNKVFAKIAADMAKPNGIK 164
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....
gi 1952248250 184 IVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFG 237
Cdd:PRK01216 165 VIDDEEVKRFINELDIADIPGIGDITAEKLKKLGVNKLVDTLRIEFDELKGIIG 218
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
28-357 |
1.19e-38 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 142.45 E-value: 1.19e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 28 LMDLDAFFASVEMLDHPEWRGKPLIVggdADSRGVVSTcSYEARRFGVHSAMASAEARRLCPQ------AIWTHGH---- 97
Cdd:cd01702 2 HIDMDAFFAQVEQVRLGLLRNDPVAV---VQWNSIIAV-SYAARAFGVTRFMTIDEAKKKCPDlilahvATYKKGEdead 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 98 -------------FDRYREVSAQVMAILADETPRVERVSIDEAFIDITpGRfapedpllVARRISDRV-AALGVTCSIGV 163
Cdd:cd01702 78 yhenpsparhkvsLDPYRRASRKILNILKRFGDVVEKASIDEAYLDLG-SR--------IVEEIRQQVyDELGYTCSAGI 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 164 GCNKTVAKIASERDKPFGLTIVRPGTEQRFLAALPVSAMSGI-GRSAEERLRRMRIYTLGELS--RAPESTLASIFGV-N 239
Cdd:cd01702 149 AHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLPITSIRGLgGKLGEEIIDLLGLPTEGDVAgfRSSESDLQEHFGEkL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 240 GERMRQRALGLEVSEVTsldeER-EVKSVSNERTFA-KDLTERGDIEAAIALLGESVGRRLR----RQGLTGATVTLKLK 313
Cdd:cd01702 229 GEWLYNLLRGIDHEPVK----PRpLPKSMGSSKNFPgKTALSTEDVQHWLLVLASELNSRLEddryENNRRPKTLVLSLR 304
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 1952248250 314 ySYGSGRTAQRRLPHPTDDENIFVAVALELLDNIWQEGMHVRLA 357
Cdd:cd01702 305 -QRGDGVRRSRSCALPRYDAQKIVKDAFKLIKAINEEGLGLAWN 347
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
28-412 |
6.89e-34 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 131.04 E-value: 6.89e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 28 LMDLDAFFASVEMLDHPEWRGKPLIVGGDADsrGVVSTCSYEARRFGVhsAMASA-----EARRLCPQAIWThGHFDRYR 102
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVLSNND--GCVIARSAEAKALGI--KMGDPwfkqkDLFRRCGVVCFS-SNYELYA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 103 EVSAQVMAILADETPRVERVSIDEAFIDITPGRFApEDPLLVARRISDRV-AALGVTCSIGVGCNKTVAKIAS----ERD 177
Cdd:PRK03609 79 DMSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNC-RDLTDFGREIRATVlQRTHLTVGVGIAQTKTLAKLANhaakKWQ 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 178 KPFG--LTIVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGlevSEV 255
Cdd:PRK03609 158 RQTGgvVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRG---EPC 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 256 TSLDEEREVKS-VSNERTFAKDLTERGDIEAAIALLGESVGRRLR--RQGLTGATVTLK-----LKYSYgSGRTAQRRLP 327
Cdd:PRK03609 235 LSLEEFAPTKQeIVCSRSFGERITDYESMRQAICSYAARAAEKLRgeHQYCRFISTFVKtspfaLNEPY-YGNSASVKLL 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 328 HPTDDENIFVAVALELLDNIWQEGMHVRLAGIGMSDFNHQGGIQTDLFCEVDGRgaQSSDRrdLSVAIDAVRDKFGDTAL 407
Cdd:PRK03609 314 TPTQDSRDIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPR--PGSEQ--LMKVLDTLNAKSGKGTL 389
|
....*
gi 1952248250 408 SFGRQ 412
Cdd:PRK03609 390 YFAGQ 394
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
30-344 |
2.06e-31 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 123.35 E-value: 2.06e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 30 DLDAFFASVEMLDHPEWRGKPLIVggdaDSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGH-FDRYREVSAQV 108
Cdd:cd01703 4 DLDCFYAQVEEIRDPSLKSKPLGI----QQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEdLTPFRDMSKKV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETP--RVERVSIDEAFIDITPGRfapedpLLVARRISDRV-----AALGVTCSIGVGCNKTVAKIASERDKPFG 181
Cdd:cd01703 80 YRLLRSYSWndRVERLGFDENFMDVTEMR------LLVASHIAYEMrerieNELGLTCCAGIASNKLLAKLVGSVNKPNQ 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 182 LTIVRP---GTEQRFLAALPVSAMSGIGRSAEERLR---------------RMRIYTLGELSRAPESTLASIFG-VNGER 242
Cdd:cd01703 154 QTTLLPpscADLMDFMDLHDLRKIPGIGYKTAAKLEahgissvrdlqefsnRNRQTVGAAPSLLELLLMVKEFGeGIGQR 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 243 MRQRALGLEVSEV-------TSLDEEREVK--SVSNERTFAKDLTER-GDIEAAIALLGESVGRRLRRQGLTGATVTLK- 311
Cdd:cd01703 234 IWKLLFGRDTSPVkpasdfpQQISIEDSYKkcSLEEIREARNKIEELlASLLERMKQDLQEVKAGDGRRPHTLRLTLRRy 313
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 1952248250 312 --LKYSYGSgRTAQRRLP-----HPTDDENIFVAVALELL 344
Cdd:cd01703 314 tsTKKHYNR-ESKQAPIPshvfqKLTGGNEIAARPIEKIL 352
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
265-364 |
4.65e-30 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 111.88 E-value: 4.65e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 265 KSVSNERTFAKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELL 344
Cdd:pfam11799 2 KSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFRTITRSVTLPSPTDDTDEIYRAALRLL 81
|
90 100
....*....|....*....|
gi 1952248250 345 DNIWQeGMHVRLAGIGMSDF 364
Cdd:pfam11799 82 RRLYR-GRPVRLLGVSLSNL 100
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
29-332 |
2.50e-22 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 99.32 E-value: 2.50e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 29 MDLDAFFASVEMLDHPEWRGKPLIVGgdadSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWTHGHFDRYREVSAQV 108
Cdd:PTZ00205 138 LDMDMFYAAVEIKKHPEYAAIPLAIG----TMTMLQTANYVARGRGIRQGMPGFLALKICPNLLILPPDFDAYNEESNTV 213
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 109 MAILADETPRVERVSIDE------AFIDITPGRFAPEDpllVARRISDRV-AALGVTCSIGVGCNKTVAKIASERDKPFG 181
Cdd:PTZ00205 214 RRIVAEYDPNYISFGLDEltlevsAYIERFEGTKTAED---VASELRVRVfGETKLTASAGIGPTAALAKIASNINKPNG 290
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 182 ---LTIVRPGTEQRFLAALPVSAMSGIGRSAEERLRRMRIYTLGEL-SRAPEstLASIFGVNGERMRQRA-LGL------ 250
Cdd:PTZ00205 291 qhdLNLHTRGDVMTYVRDLGLRSVPGVGKVTEALLKGLGITTLSDIyNRRVE--LCYILHNNLFRFLLGAsIGImqwpda 368
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 251 -EVSEVTSLDEEREV--KSVSNERTFaKDLTERGDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLP 327
Cdd:PTZ00205 369 aTAANTENCEGATGGqrKAISSERSF-TTPRTKEGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWASYRYQQYTKSLI 447
|
....*
gi 1952248250 328 HPTDD 332
Cdd:PTZ00205 448 QYSDD 452
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
42-345 |
3.66e-20 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 90.90 E-value: 3.66e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 42 DHPEWRGKPLIVGgDADSRGVVSTCSYEARRFGVHSAMASAEARRLCPQAIWthghFDRYREVSAQVMAILADE----TP 117
Cdd:cd03468 16 NRPADDEAPLAVV-ERKKAGRILACNAAARAAGVRPGMPLAEALALCPNLQV----VEYDPEADARALQELALWllrfTP 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 118 RVERVSIDEAFIDITP-GRFAPEDPLLvARRISDRVAALGVTCSIGVGCNKTVAKIASERDKPFGLT-IVRPGTEQRFLA 195
Cdd:cd03468 91 LVALDGPDGLLLDVTGcLHLFGGEDAL-AASLRAALATLGLSARAGIADTPGAAWLLARAGGGRGVLrREALAAALVLLA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1952248250 196 ALPVSAmSGIGRSAEERLRRMRIYTLGELSRAPESTLASIFGVNGERMRQRALGlevSEVTSLDEE--REVKSVSNERTF 273
Cdd:cd03468 170 PLPVAA-LRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQAYG---RDPEPLLFSppPPAFDFRLELQL 245
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1952248250 274 AKDLTErgDIEAAIALLGESVGRRLRRQGLTGATVTLKLKYSYGSGRTAQRRLPHPTDDENIFVAVALELLD 345
Cdd:cd03468 246 EEPIAR--GLLFPLRRLLEQLCAFLALRGLGARRLSLTLFREDGRVTRVLVGLARPSRDDLPLLRLLRERLE 315
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
187-216 |
3.50e-03 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 34.68 E-value: 3.50e-03
10 20 30
....*....|....*....|....*....|
gi 1952248250 187 PGTEQRFLAALPVSAMSGIGRSAEERLRRM 216
Cdd:pfam11798 1 PDDVPEFLWPLPISKIPGIGKKLAEKLKAL 30
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|
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