NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1956526470|ref|WP_200404836|]
View 

iron-sulfur cluster assembly accessory protein [Francisella hispaniensis]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 12-118 6.80e-36

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 118.33  E-value: 6.80e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  12 VLEVTDAAAKHFKKHLEKHNG-CIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:COG0316     1 PITLTDAAAKRIKRLLAKEGNpGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:COG0316    81 EELLG-SGFKFNNPNAKSSCGCGESFSV 107
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 12-118 6.80e-36

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 118.33  E-value: 6.80e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  12 VLEVTDAAAKHFKKHLEKHNG-CIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:COG0316     1 PITLTDAAAKRIKRLLAKEGNpGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:COG0316    81 EELLG-SGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 14-118 4.55e-24

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 88.41  E-value: 4.55e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  14 EVTDAAAKHFKKHLE---KHNGCIGIYVGTKvmGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:TIGR00049   1 TLTDSAAKRIKALLAgegEPNLGLRVGVKGG--GCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYV 78

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:TIGR00049  79 EELLG-SGFTFTNPNAKGTCGCGKSFSV 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 2-118 5.53e-21

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 80.94  E-value: 5.53e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470   2 VEVFDL--NASSVLEVTDAAAKHFKKHLEKHNGCIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSM 79
Cdd:PRK09504    5 SGTFNPddFAWQGLTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAM 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1956526470  80 NFLNGLKIDYVKHdfGLYKL-EYTNPNESARCGCGESFTV 118
Cdd:PRK09504   85 PFIDGTEVDYVRE--GLNQIfKFHNPKAQNECGCGESFGV 122
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 13-114 1.11e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 64.59  E-value: 1.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  13 LEVTDAAAKHFKKHLEKHNGCIGIYV---GTKVM--GCS-GLAYDVDFVKQ-QPEDTQKVQQHGISFFVSNKSMNFL-NG 84
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddgGGPYSkgGCSiGGKFSLVLVDEpDPDYDEVIESNGGPIYVDSYSLPFLdEG 82

                          90       100       110
                  ....*....|....*....|....*....|
gi 1956526470  85 LKIDYVKHDFGLyKLEYTNPNESARCGCGE 114
Cdd:pfam01521  83 LTLDFVEDLGTL-GLKSDNGNLDGNVGCGD 111
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 12-118 6.80e-36

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 118.33  E-value: 6.80e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  12 VLEVTDAAAKHFKKHLEKHNG-CIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:COG0316     1 PITLTDAAAKRIKRLLAKEGNpGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:COG0316    81 EELLG-SGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 14-118 4.55e-24

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 88.41  E-value: 4.55e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  14 EVTDAAAKHFKKHLE---KHNGCIGIYVGTKvmGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:TIGR00049   1 TLTDSAAKRIKALLAgegEPNLGLRVGVKGG--GCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYV 78

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:TIGR00049  79 EELLG-SGFTFTNPNAKGTCGCGKSFSV 105
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 11-118 1.74e-23

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 86.81  E-value: 1.74e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  11 SVLEVTDAAAKHFKKHLEKHNGCIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYV 90
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRPEAVGIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDFV 80

                          90       100
                  ....*....|....*....|....*...
gi 1956526470  91 KHDFGlYKLEYTNPNESARCGCGESFTV 118
Cdd:TIGR01997  81 RTTLR-QGFKFNNPNATSACGCGESFEL 107
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 15-118 8.77e-23

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 85.26  E-value: 8.77e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  15 VTDAAAKHFKKHLEKHNGCIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYVKHdf 94
Cdd:TIGR02011   3 LTDSAAARVNTFLANRGKGFGLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVKE-- 80

                          90       100
                  ....*....|....*....|....*
gi 1956526470  95 GLYK-LEYTNPNESARCGCGESFTV 118
Cdd:TIGR02011  81 GLNEgFKFTNPNVKDECGCGESFHV 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 2-118 5.53e-21

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 80.94  E-value: 5.53e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470   2 VEVFDL--NASSVLEVTDAAAKHFKKHLEKHNGCIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSM 79
Cdd:PRK09504    5 SGTFNPddFAWQGLTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAM 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1956526470  80 NFLNGLKIDYVKHdfGLYKL-EYTNPNESARCGCGESFTV 118
Cdd:PRK09504   85 PFIDGTEVDYVRE--GLNQIfKFHNPKAQNECGCGESFGV 122
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
13-118 1.46e-19

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 76.83  E-value: 1.46e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  13 LEVTDAAAKHFKKHLEKHNGCIGIYVGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYVKH 92
Cdd:PRK09502    3 ITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82

                          90       100
                  ....*....|....*....|....*..
gi 1956526470  93 dfGLYK-LEYTNPNESARCGCGESFTV 118
Cdd:PRK09502   83 --GLNEgFKFTNPNVKDECGCGESFHV 107
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 13-114 1.11e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 64.59  E-value: 1.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  13 LEVTDAAAKHFKKHLEKHNGCIGIYV---GTKVM--GCS-GLAYDVDFVKQ-QPEDTQKVQQHGISFFVSNKSMNFL-NG 84
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddgGGPYSkgGCSiGGKFSLVLVDEpDPDYDEVIESNGGPIYVDSYSLPFLdEG 82

                          90       100       110
                  ....*....|....*....|....*....|
gi 1956526470  85 LKIDYVKHDFGLyKLEYTNPNESARCGCGE 114
Cdd:pfam01521  83 LTLDFVEDLGTL-GLKSDNGNLDGNVGCGD 111
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 13-118 1.99e-09

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 51.08  E-value: 1.99e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  13 LEVTDAAAKHFKKHLE---KHNGCIGIYV--GtkvmGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKI 87
Cdd:PRK13623   10 LVFTDAAAAKVKELIEeegNPDLKLRVYItgG----GCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEV 85

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1956526470  88 DYVKhdfGL--YKLEYTNPNESARCGCGESFTV 118
Cdd:PRK13623   86 DYTE---GLegSRFVIKNPNAKTTCGCGSSFSI 115
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 38-118 9.29e-07

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 44.92  E-value: 9.29e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  38 VGTKVMGCSGLAYDVDFVKQQPEDTQKVQQHGISFFVSNKSMNFLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFT 117
Cdd:PLN03082   82 LSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVDYVEELIRSAFVVSTNPSAVGGCSCKSSFM 161


                  .
gi 1956526470 118 V 118
Cdd:PLN03082  162 V 162
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
13-109 1.62e-04

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 39.23  E-value: 1.62e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1956526470  13 LEVTDAAAKHFKKHLEKH--NGCIGIYV---GTKVMGCsGLAY-DVDFVkqQPEDTQkVQQHGISFFVSNKSMNFLNGLK 86
Cdd:PRK11190    2 ITISDAAQAHFAKLLANQeeGTQIRVFVinpGTPNAEC-GVSYcPPDAV--EATDTE-LKFDGFSAYVDELSAPFLEDAE 77

                          90       100
                  ....*....|....*....|...
gi 1956526470  87 IDYVKHDFGlYKLEYTNPNESAR 109
Cdd:PRK11190   78 IDFVTDQLG-SQLTLKAPNAKMR 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH