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Conserved domains on  [gi|2107271840|ref|WP_225540019|]
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MULTISPECIES: GNAT family N-acetyltransferase [Empedobacter]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11450351)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
16-150 5.10e-54

Predicted N-acyltransferase, GNAT family [General function prediction only];


:

Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 166.51  E-value: 5.10e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  16 SKELYKIIQLRNDVFVIEQD-CIYQDCDGKDLVCGHLWATIDNEIAAYSRIVPQGisyENEPSIGRVITNPKFRRYGLGK 94
Cdd:COG2153     1 AEELYDALALRREVFVVEQGvPPYLELDGKDEDARHLLAYDDGELVATARLLPPG---DGEAKIGRVAVLPEYRGQGLGR 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2107271840  95 QLMKNSVQVIENQfKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:COG2153    78 ALMEAAIEEARER-GARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKP 132
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
16-150 5.10e-54

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 166.51  E-value: 5.10e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  16 SKELYKIIQLRNDVFVIEQD-CIYQDCDGKDLVCGHLWATIDNEIAAYSRIVPQGisyENEPSIGRVITNPKFRRYGLGK 94
Cdd:COG2153     1 AEELYDALALRREVFVVEQGvPPYLELDGKDEDARHLLAYDDGELVATARLLPPG---DGEAKIGRVAVLPEYRGQGLGR 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2107271840  95 QLMKNSVQVIENQfKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:COG2153    78 ALMEAAIEEARER-GARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKP 132
PRK10314 PRK10314
GNAT family N-acetyltransferase;
4-150 1.02e-33

GNAT family N-acetyltransferase;


Pssm-ID: 182373 [Multi-domain]  Cd Length: 153  Bit Score: 115.71  E-value: 1.02e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840   4 IIWHFKSFEELTSKELYKIIQLRNDVFVIEQDCIYQDCDGKDLVCG--HLWATIDNEIAAYSRIVPQgiSYENEPS-IGR 80
Cdd:PRK10314    2 IEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDnrHILGWKNDELVAYARILKS--DDDLEPVvIGR 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  81 VITNPKFRRYGLGKQLMKNSVQVIENQFKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:PRK10314   80 VIVSEALRGEKVGQQLMSKTLESCTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARE 149
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 32-150 8.73e-14

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 63.83  E-value: 8.73e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  32 IEQDCIYQDCDGKDLvcgHLW-ATIDNEIAAYsrivpqgISYENEPSIGRVITNPKFRRYGLGKQLMKNSVQVIENQF-K 109
Cdd:pfam13673  17 ISPEALRERIDQGEY---FFFvAFEGGQIVGV-------IALRDRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGiK 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2107271840 110 TSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:pfam13673  87 LSELTVNASPYAVPFYEKLGFRATGPEQEFNGIRFVPMEKE 127
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-98 1.19e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 1.19e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 2107271840  50 HLWATIDNEIAAYSRIVPQGiSYENEPSIGRVITNPKFRRYGLGKQLMK 98
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDG-SGGDTAYIGDLAVLPEYRGKGIGSALLE 48
 
Name Accession Description Interval E-value
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
16-150 5.10e-54

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 166.51  E-value: 5.10e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  16 SKELYKIIQLRNDVFVIEQD-CIYQDCDGKDLVCGHLWATIDNEIAAYSRIVPQGisyENEPSIGRVITNPKFRRYGLGK 94
Cdd:COG2153     1 AEELYDALALRREVFVVEQGvPPYLELDGKDEDARHLLAYDDGELVATARLLPPG---DGEAKIGRVAVLPEYRGQGLGR 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2107271840  95 QLMKNSVQVIENQfKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:COG2153    78 ALMEAAIEEARER-GARRIVLSAQAHAVGFYEKLGFVPVGEEFLEAGIPHIDMRKP 132
PRK10314 PRK10314
GNAT family N-acetyltransferase;
4-150 1.02e-33

GNAT family N-acetyltransferase;


Pssm-ID: 182373 [Multi-domain]  Cd Length: 153  Bit Score: 115.71  E-value: 1.02e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840   4 IIWHFKSFEELTSKELYKIIQLRNDVFVIEQDCIYQDCDGKDLVCG--HLWATIDNEIAAYSRIVPQgiSYENEPS-IGR 80
Cdd:PRK10314    2 IEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDnrHILGWKNDELVAYARILKS--DDDLEPVvIGR 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  81 VITNPKFRRYGLGKQLMKNSVQVIENQFKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:PRK10314   80 VIVSEALRGEKVGQQLMSKTLESCTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMARE 149
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 32-150 8.73e-14

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 63.83  E-value: 8.73e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  32 IEQDCIYQDCDGKDLvcgHLW-ATIDNEIAAYsrivpqgISYENEPSIGRVITNPKFRRYGLGKQLMKNSVQVIENQF-K 109
Cdd:pfam13673  17 ISPEALRERIDQGEY---FFFvAFEGGQIVGV-------IALRDRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGiK 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2107271840 110 TSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:pfam13673  87 LSELTVNASPYAVPFYEKLGFRATGPEQEFNGIRFVPMEKE 127
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
11-150 4.71e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 51.62  E-value: 4.71e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  11 FEELTSKELYKIIQLRNDVFVI--EQDCIYQDCDGKDLVCGhLWATIDNEIAAYSRIVPQGISYE-NEPSIGRVITNPKF 87
Cdd:COG3153     1 IRPATPEDAEAIAALLRAAFGPgrEAELVDRLREDPAAGLS-LVAEDDGEIVGHVALSPVDIDGEgPALLLGPLAVDPEY 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2107271840  88 RRYGLGKQLMKNSVQVIENQfKTSSIRISAQSYLKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:COG3153    80 RGQGIGRALMRAALEAARER-GARAVVLLGDPSLLPFYERFGFRPAGELGLTLGPDEVFLAKE 141
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 49-138 9.77e-06

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 42.67  E-value: 9.77e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  49 GHLW-ATIDNEIAAYSRIVPQGisyENEPSIGRVITNPKFRRYGLGKQLMknsvQVIENQFKTSSI-RISAQSYLK--EF 124
Cdd:COG1246    28 GEFWvAEEDGEIVGCAALHPLD---EDLAELRSLAVHPDYRGRGIGRRLL----EALLAEARELGLkRLFLLTTSAaiHF 100

                          90
                  ....*....|....
gi 2107271840 125 YSTFGFKQVSEEYL 138
Cdd:COG1246   101 YEKLGFEEIDKEDL 114
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 49-132 2.25e-05

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 40.52  E-value: 2.25e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  49 GHLW-ATIDNEIAAYSRIVPQGISYENEpsIGRVITNPKFRRYGLGKQLMKNsVQVIENQFKTSSIRISAQSYLKEFYST 127
Cdd:pfam13508   3 GRFFvAEDDGKIVGFAALLPLDDEGALA--ELRLAVHPEYRGQGIGRALLEA-AEAAAKEGGIKLLELETTNRAAAFYEK 79


                  ....*
gi 2107271840 128 FGFKQ 132
Cdd:pfam13508  80 LGFEE 84
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-98 1.19e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 1.19e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 2107271840  50 HLWATIDNEIAAYSRIVPQGiSYENEPSIGRVITNPKFRRYGLGKQLMK 98
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDG-SGGDTAYIGDLAVLPEYRGKGIGSALLE 48
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 78-150 1.06e-03

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 36.17  E-value: 1.06e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2107271840  78 IGRVITNPKFRRYGLGKQLMKNSVQ-VIENQFKTSSIRISAQSY-LKEFYSTFGFKQVSEEYLEDDIPHIEMLRK 150
Cdd:COG0456    16 IEDLAVDPEYRGRGIGRALLEAALErARERGARRLRLEVREDNEaAIALYEKLGFEEVGERPNYYGDDALVMEKE 90
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 49-130 1.25e-03

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 36.34  E-value: 1.25e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2107271840  49 GHLWATIDNEIAAYSRIVPQGiSYENEPSIGRVITNPKFRRYGLGKQLMKNSVQV-IENQFKTSSIRI-SAQSYLKEFYS 126
Cdd:pfam00583  34 GFFVAEEDGELVGFASLSIID-DEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWaRERGCERIFLEVaADNLAAIALYE 112


                  ....
gi 2107271840 127 TFGF 130
Cdd:pfam00583 113 KLGF 116
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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