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Conserved domains on  [gi|2156035088|ref|WP_230247309|]
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MULTISPECIES: ribonuclease domain-containing protein [Rubrivivax]

Protein Classification

COG4290 family protein( domain architecture ID 10008463)

COG4290 family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
COG4290 COG4290
Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];
2-113 7.58e-56

Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];


:

Pssm-ID: 443431 [Multi-domain]  Cd Length: 124  Bit Score: 169.37  E-value: 7.58e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088   2 ACGAAVHARPSSPPAEAA-DVVSLNELPAQAQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARN 80
Cdd:COG4290    17 LAGCTASAPGSAPTSSSGlPTVALSELPPEARDTLALIEAGGPFPYRKDGVVFGNRERLLPAKPRGYYREYTVPTPGSRD 96

                          90       100       110
                  ....*....|....*....|....*....|...
gi 2156035088  81 RGARRIVCGGqqptaPDTCFYTADHYASFSRIA 113
Cdd:COG4290    97 RGARRIVTGG-----DGEAYYTDDHYASFRRIR 124
 
Name Accession Description Interval E-value
COG4290 COG4290
Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];
2-113 7.58e-56

Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];


Pssm-ID: 443431 [Multi-domain]  Cd Length: 124  Bit Score: 169.37  E-value: 7.58e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088   2 ACGAAVHARPSSPPAEAA-DVVSLNELPAQAQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARN 80
Cdd:COG4290    17 LAGCTASAPGSAPTSSSGlPTVALSELPPEARDTLALIEAGGPFPYRKDGVVFGNRERLLPAKPRGYYREYTVPTPGSRD 96

                          90       100       110
                  ....*....|....*....|....*....|...
gi 2156035088  81 RGARRIVCGGqqptaPDTCFYTADHYASFSRIA 113
Cdd:COG4290    97 RGARRIVTGG-----DGEAYYTDDHYASFRRIR 124
RNase_Sa cd00607
RNase_Sa. Ribonucleases first isolated from Streptomyces aureofaciens. In general, ...
 22-113 8.22e-49

RNase_Sa. Ribonucleases first isolated from Streptomyces aureofaciens. In general, ribonucleases cleave phosphodiester bonds in RNA and are essential for both non-specific RNA degradation and for numerous forms of RNA processing. RNAse Sa is a guanylate specific endoribonuclease which belongs to the superfamily of microbial ribonucleases. Typical of this sub-family, the enzyme hydrolyses the phosphodiester bonds of RNA at the 3' oxygen end of guanosine residues to yield oligonucleotides with the guanosine-2',3'-cyclophosphate at the 3' end and the hydroxyl group at the 5' end. The terminal guanosine-2,3'-cyclophosphate is hydrolysed by guanyl RNAses to give guanosine-3'-phosphate.


Pssm-ID: 238340  Cd Length: 95  Bit Score: 150.61  E-value: 8.22e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088  22 VSLNELPAQAQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARNRGARRIVCGGQQPtAPDTCFY 101
Cdd:cd00607     5 VCLAQLPPEAQDTLNLIAKGGPFPYSKDGVVFGNREGRLPKQSRGYYHEYTVKTPGSRDRGARRIVCGGPPR-DTGECYY 83

                          90
                  ....*....|..
gi 2156035088 102 TADHYASFSRIA 113
Cdd:cd00607    84 TDDHYASFKRID 95
Ribonuclease pfam00545
ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides.
 34-111 8.33e-36

ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides.


Pssm-ID: 459849  Cd Length: 81  Bit Score: 117.35  E-value: 8.33e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088  34 TYRLIHAG---GPFPY-QKDGTVFFNRERLLPAKARGYYREYTVPTPGARNRGARRIVCGGQQPtaPDTCFYTADHYASF 109
Cdd:pfam00545   2 TLEAIEAGwwpGPFPYpPDGGDVFGNREGRLPQAPGGYYREYDVNTPGGRYRGARRIVTGDGLD--GGEIYYTADHYNSF 79


                  ..
gi 2156035088 110 SR 111
Cdd:pfam00545  80 VR 81
 
Name Accession Description Interval E-value
COG4290 COG4290
Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];
2-113 7.58e-56

Guanyl-specific ribonuclease Sa [Nucleotide transport and metabolism];


Pssm-ID: 443431 [Multi-domain]  Cd Length: 124  Bit Score: 169.37  E-value: 7.58e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088   2 ACGAAVHARPSSPPAEAA-DVVSLNELPAQAQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARN 80
Cdd:COG4290    17 LAGCTASAPGSAPTSSSGlPTVALSELPPEARDTLALIEAGGPFPYRKDGVVFGNRERLLPAKPRGYYREYTVPTPGSRD 96

                          90       100       110
                  ....*....|....*....|....*....|...
gi 2156035088  81 RGARRIVCGGqqptaPDTCFYTADHYASFSRIA 113
Cdd:COG4290    97 RGARRIVTGG-----DGEAYYTDDHYASFRRIR 124
RNase_Sa cd00607
RNase_Sa. Ribonucleases first isolated from Streptomyces aureofaciens. In general, ...
 22-113 8.22e-49

RNase_Sa. Ribonucleases first isolated from Streptomyces aureofaciens. In general, ribonucleases cleave phosphodiester bonds in RNA and are essential for both non-specific RNA degradation and for numerous forms of RNA processing. RNAse Sa is a guanylate specific endoribonuclease which belongs to the superfamily of microbial ribonucleases. Typical of this sub-family, the enzyme hydrolyses the phosphodiester bonds of RNA at the 3' oxygen end of guanosine residues to yield oligonucleotides with the guanosine-2',3'-cyclophosphate at the 3' end and the hydroxyl group at the 5' end. The terminal guanosine-2,3'-cyclophosphate is hydrolysed by guanyl RNAses to give guanosine-3'-phosphate.


Pssm-ID: 238340  Cd Length: 95  Bit Score: 150.61  E-value: 8.22e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088  22 VSLNELPAQAQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARNRGARRIVCGGQQPtAPDTCFY 101
Cdd:cd00607     5 VCLAQLPPEAQDTLNLIAKGGPFPYSKDGVVFGNREGRLPKQSRGYYHEYTVKTPGSRDRGARRIVCGGPPR-DTGECYY 83

                          90
                  ....*....|..
gi 2156035088 102 TADHYASFSRIA 113
Cdd:cd00607    84 TDDHYASFKRID 95
Ribonuclease pfam00545
ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides.
 34-111 8.33e-36

ribonuclease; This enzyme hydrolyses RNA and oligoribonucleotides.


Pssm-ID: 459849  Cd Length: 81  Bit Score: 117.35  E-value: 8.33e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2156035088  34 TYRLIHAG---GPFPY-QKDGTVFFNRERLLPAKARGYYREYTVPTPGARNRGARRIVCGGQQPtaPDTCFYTADHYASF 109
Cdd:pfam00545   2 TLEAIEAGwwpGPFPYpPDGGDVFGNREGRLPQAPGGYYREYDVNTPGGRYRGARRIVTGDGLD--GGEIYYTADHYNSF 79


                  ..
gi 2156035088 110 SR 111
Cdd:pfam00545  80 VR 81
microbial_RNases cd00389
microbial_RNases. Ribonucleases (RNAses) cleave phosphodiester bonds in RNA and are essential ...
 31-106 6.13e-25

microbial_RNases. Ribonucleases (RNAses) cleave phosphodiester bonds in RNA and are essential for both non-specific RNA degradation and for numerous forms of RNA processing. The alignment contains fungal RNases (U2, T1, F1, Th, Pb, N1, and Ms) and bacterial RNases (barnase, binase, RNase Sa) , the majority of which are guanyl specific and fungal ribotoxins.


Pssm-ID: 238228  Cd Length: 71  Bit Score: 89.60  E-value: 6.13e-25
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2156035088  31 AQKTYRLIHAGGPFPYQKDGTVFFNRERLLPAKARGYYREYTVPTPGARNRGARRIVCGGqqptaPDTCFYTADHY 106
Cdd:cd00389     1 AQAALNLIASGGPFGYSIYGHVFNNREGFLPFKGSGYYHEYPVNTPGGGSRGADRVVYGG-----DGEFYGTIDHY 71
barnase cd00933
Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage ...
 50-112 2.52e-05

Barnase, a member of the family of homologous microbial ribonucleases, catalyses the cleavage of single-stranded RNA via a two-step mechanism thought to be similar to that of pancreatic ribonuclease. The mechanism involves a transesterification to give a 2', 3'-cyclic phosphate intermediate, followed by hydrolysis to yield a 3' nucleotide. The active site residues His and Glu act as general acid-base groups during catalysis, while the Arg and Lys residues are important in binding the reactive phosphate, the latter probably binding the phosphate in the transition state. Barstar, a small 89 residue intracellular protein is a natural inhibitor of Barnase.


Pssm-ID: 238471 [Multi-domain]  Cd Length: 107  Bit Score: 40.18  E-value: 2.52e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2156035088  50 GTVFFNRERLLPAKARGYYREYTVPTPGARnRGARRIVcggqqpTAPDTCFY-TADHYASFSRI 112
Cdd:cd00933    50 GDRFSNREGRLPAAGGRTWREADINYQGGH-RGADRLL------YSNDGLIYkTTDHYRTFTRI 106
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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