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Conserved domains on  [gi|2240906815|ref|WP_249540682|]
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dihydrofolate reductase, partial [Escherichia coli]

Protein Classification

dihydrofolate reductase family protein( domain architecture ID 106942)

dihydrofolate reductase family protein; similar to Lacticaseibacillus rhamnosus dihydrofolate reductase which reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DHFR super family cl17279
Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with ...
 9-43 2.43e-14

Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5,10-methylenetetrahydrofolate which is then utilized by thymidylate synthase. Inhibition of DHFR interrupts thymidilate synthesis and DNA replication, inhibitors of DHFR (such as Methotrexate) are used in cancer chemotherapy. 5,6,7,8-tetrahydrofolate also is involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis.


The actual alignment was detected with superfamily member pfam00186:

Pssm-ID: 473077 [Multi-domain]  Cd Length: 159  Bit Score: 61.79  E-value: 2.43e-14
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2240906815   9 KVSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:pfam00186   1 MISLIAAMDENGVIGKDNDLPWHLPADLKHFKKLT 35
 
Name Accession Description Interval E-value
DHFR_1 pfam00186
Dihydrofolate reductase;
9-43 2.43e-14

Dihydrofolate reductase;


Pssm-ID: 425512 [Multi-domain]  Cd Length: 159  Bit Score: 61.79  E-value: 2.43e-14
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2240906815   9 KVSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:pfam00186   1 MISLIAAMDENGVIGKDNDLPWHLPADLKHFKKLT 35
DHFR cd00209
Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with ...
 10-43 2.62e-13

Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5,10-methylenetetrahydrofolate which is then utilized by thymidylate synthase. Inhibition of DHFR interrupts thymidilate synthesis and DNA replication, inhibitors of DHFR (such as Methotrexate) are used in cancer chemotherapy. 5,6,7,8-tetrahydrofolate also is involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis.


Pssm-ID: 238127 [Multi-domain]  Cd Length: 158  Bit Score: 59.07  E-value: 2.62e-13
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2240906815  10 VSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:cd00209     1 ISLIVAVDENGVIGKDNKLPWHLPEDLKHFKKTT 34
PTZ00164 PTZ00164
bifunctional dihydrofolate reductase-thymidylate synthase; Provisional
 10-43 1.87e-10

bifunctional dihydrofolate reductase-thymidylate synthase; Provisional


Pssm-ID: 240299 [Multi-domain]  Cd Length: 514  Bit Score: 52.75  E-value: 1.87e-10
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2240906815  10 VSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:PTZ00164   10 FSIVVAVTLKRGIGIGNSLPWHIPEDMKFFSKIT 43
FolA COG0262
Dihydrofolate reductase [Coenzyme transport and metabolism]; Dihydrofolate reductase is part ...
 8-43 6.07e-05

Dihydrofolate reductase [Coenzyme transport and metabolism]; Dihydrofolate reductase is part of the Pathway/BioSystem: Folate biosynthesis


Pssm-ID: 440032 [Multi-domain]  Cd Length: 168  Bit Score: 37.14  E-value: 6.07e-05
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 2240906815   8 MKVSLIVAMDLEKGIG-KNNDLMWHL--PADMLFFKETT 43
Cdd:COG0262     1 RKLILIVAVSLDGVIGgPDGDLPWLFpdPEDLAHFKELT 39
 
Name Accession Description Interval E-value
DHFR_1 pfam00186
Dihydrofolate reductase;
9-43 2.43e-14

Dihydrofolate reductase;


Pssm-ID: 425512 [Multi-domain]  Cd Length: 159  Bit Score: 61.79  E-value: 2.43e-14
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2240906815   9 KVSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:pfam00186   1 MISLIAAMDENGVIGKDNDLPWHLPADLKHFKKLT 35
DHFR cd00209
Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with ...
 10-43 2.62e-13

Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5,10-methylenetetrahydrofolate which is then utilized by thymidylate synthase. Inhibition of DHFR interrupts thymidilate synthesis and DNA replication, inhibitors of DHFR (such as Methotrexate) are used in cancer chemotherapy. 5,6,7,8-tetrahydrofolate also is involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis.


Pssm-ID: 238127 [Multi-domain]  Cd Length: 158  Bit Score: 59.07  E-value: 2.62e-13
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2240906815  10 VSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:cd00209     1 ISLIVAVDENGVIGKDNKLPWHLPEDLKHFKKTT 34
PTZ00164 PTZ00164
bifunctional dihydrofolate reductase-thymidylate synthase; Provisional
 10-43 1.87e-10

bifunctional dihydrofolate reductase-thymidylate synthase; Provisional


Pssm-ID: 240299 [Multi-domain]  Cd Length: 514  Bit Score: 52.75  E-value: 1.87e-10
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2240906815  10 VSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:PTZ00164   10 FSIVVAVTLKRGIGIGNSLPWHIPEDMKFFSKIT 43
folA PRK10769
type 3 dihydrofolate reductase;
10-43 8.34e-07

type 3 dihydrofolate reductase;


Pssm-ID: 182714 [Multi-domain]  Cd Length: 159  Bit Score: 42.03  E-value: 8.34e-07
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2240906815  10 VSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETT 43
Cdd:PRK10769    2 ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNT 35
FolA COG0262
Dihydrofolate reductase [Coenzyme transport and metabolism]; Dihydrofolate reductase is part ...
 8-43 6.07e-05

Dihydrofolate reductase [Coenzyme transport and metabolism]; Dihydrofolate reductase is part of the Pathway/BioSystem: Folate biosynthesis


Pssm-ID: 440032 [Multi-domain]  Cd Length: 168  Bit Score: 37.14  E-value: 6.07e-05
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 2240906815   8 MKVSLIVAMDLEKGIG-KNNDLMWHL--PADMLFFKETT 43
Cdd:COG0262     1 RKLILIVAVSLDGVIGgPDGDLPWLFpdPEDLAHFKELT 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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