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Conserved domains on  [gi|2254456506|ref|WP_251629089|]
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MULTISPECIES: DUF86 domain-containing protein [Bacillaceae]

Protein Classification

DUF86 domain-containing protein( domain architecture ID 10006451)

uncharacterized DUF86 domain-containing protein similar to Bacillus subtilis YutE

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 2-132 7.29e-40

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


:

Pssm-ID: 441985  Cd Length: 132  Bit Score: 130.02  E-value: 7.29e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2254456506   2 NDVILNKATTIERCVKRIHEVYEDNPAN-LTDFTKQDSIILNIQRACEASIDLAMHIVSKRKLGVPKASREAFKLLLNAG 80
Cdd:COG2445     1 RERIEEKLEYIEEYLARLEELAELSPEEfLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2254456506  81 LIEKSLANTLMNMVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVILQ 132
Cdd:COG2445    81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIRK 132
 
Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 2-132 7.29e-40

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


Pssm-ID: 441985  Cd Length: 132  Bit Score: 130.02  E-value: 7.29e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2254456506   2 NDVILNKATTIERCVKRIHEVYEDNPAN-LTDFTKQDSIILNIQRACEASIDLAMHIVSKRKLGVPKASREAFKLLLNAG 80
Cdd:COG2445     1 RERIEEKLEYIEEYLARLEELAELSPEEfLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2254456506  81 LIEKSLANTLMNMVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVILQ 132
Cdd:COG2445    81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIRK 132
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
 12-130 2.36e-24

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 90.10  E-value: 2.36e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2254456506  12 IERCVKRIHEVYEDNPAN--LTDFTKQDSIILNIQRACEASIDLAMHIVSKRKLGVPkASREAFKLLLNAGLIEKSLANT 89
Cdd:pfam01934   1 ILEAIERIEEFTQGLTEEefLSDRLTQDAVERNLEIIGEAAIDIGNHLISDLGLRDP-GSYDIFDILAEEGVISEEPAIP 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2254456506  90 LMNMVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVI 130
Cdd:pfam01934  80 WRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFLEKI 120
 
Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 2-132 7.29e-40

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


Pssm-ID: 441985  Cd Length: 132  Bit Score: 130.02  E-value: 7.29e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2254456506   2 NDVILNKATTIERCVKRIHEVYEDNPAN-LTDFTKQDSIILNIQRACEASIDLAMHIVSKRKLGVPKASREAFKLLLNAG 80
Cdd:COG2445     1 RERIEEKLEYIEEYLARLEELAELSPEEfLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2254456506  81 LIEKSLANTLMNMVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVILQ 132
Cdd:COG2445    81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIRK 132
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
 12-130 2.36e-24

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 90.10  E-value: 2.36e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2254456506  12 IERCVKRIHEVYEDNPAN--LTDFTKQDSIILNIQRACEASIDLAMHIVSKRKLGVPkASREAFKLLLNAGLIEKSLANT 89
Cdd:pfam01934   1 ILEAIERIEEFTQGLTEEefLSDRLTQDAVERNLEIIGEAAIDIGNHLISDLGLRDP-GSYDIFDILAEEGVISEEPAIP 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2254456506  90 LMNMVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVI 130
Cdd:pfam01934  80 WRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFLEKI 120
HEPN COG2361
HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];
93-136 3.38e-03

HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];


Pssm-ID: 441928  Cd Length: 115  Bit Score: 34.85  E-value: 3.38e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 2254456506  93 MVGFRNIAVHDYQTLELDILEAILEKHINDFIVFTKVILQLEEN 136
Cdd:COG2361    72 IAGMRNRLAHGYFGVDLEIVWDTVQNDLPELKEQIEAILEELEE 115
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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