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Conserved domains on  [gi|2444577466|ref|WP_273596278|]
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glycine cleavage system protein GcvH [Roseateles koreensis]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.40e-66

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.88  E-value: 1.40e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   2 SNIKYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEAL 81
Cdd:COG0509     5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2444577466  82 RDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:COG0509    85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLE 126
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.40e-66

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.88  E-value: 1.40e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   2 SNIKYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEAL 81
Cdd:COG0509     5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2444577466  82 RDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:COG0509    85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLE 126
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-123 6.80e-61

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 182.67  E-value: 6.80e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   1 MSNIKYTPDHEWVQIEaDGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEA 80
Cdd:PRK01202    5 PADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEA 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2444577466  81 LRDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:PRK01202   84 LEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIE 126
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 5-123 5.57e-47

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 147.48  E-value: 5.57e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   5 KYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDD 84
Cdd:pfam01597   2 YYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLEDN 81

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 2444577466  85 PSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:pfam01597  82 PGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLK 120
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-101 7.30e-46

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 143.44  E-value: 7.30e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   6 YTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDDP 85
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 2444577466  86 SLANTDPLKAGWFFKV 101
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 4-121 7.97e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 142.28  E-value: 7.97e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   4 IKYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRD 83
Cdd:TIGR00527   6 LRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALED 85

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 2444577466  84 DPSLANTDPLKAGWFFKVKLNNAA-ELDGLMDGTAYDEL 121
Cdd:TIGR00527  86 SPELVNEDPYGDGWLIKVKLSDGEsEVEGLMSAEQYEAT 124
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.40e-66

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.88  E-value: 1.40e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   2 SNIKYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEAL 81
Cdd:COG0509     5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2444577466  82 RDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:COG0509    85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLE 126
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-123 6.80e-61

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 182.67  E-value: 6.80e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   1 MSNIKYTPDHEWVQIEaDGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEA 80
Cdd:PRK01202    5 PADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEA 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2444577466  81 LRDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:PRK01202   84 LEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIE 126
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-123 2.67e-50

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 156.32  E-value: 2.67e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   1 MSNIKYTP-DHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNE 79
Cdd:PRK13380   10 PSELRYTIkNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2444577466  80 ALRDDPSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:PRK13380   90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLK 133
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 5-123 5.57e-47

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 147.48  E-value: 5.57e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   5 KYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDD 84
Cdd:pfam01597   2 YYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLEDN 81

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 2444577466  85 PSLANTDPLKAGWFFKVKLNNAAELDGLMDGTAYDELVK 123
Cdd:pfam01597  82 PGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLK 120
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-101 7.30e-46

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 143.44  E-value: 7.30e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   6 YTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDDP 85
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 2444577466  86 SLANTDPLKAGWFFKV 101
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 4-121 7.97e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 142.28  E-value: 7.97e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   4 IKYTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRD 83
Cdd:TIGR00527   6 LRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALED 85

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 2444577466  84 DPSLANTDPLKAGWFFKVKLNNAA-ELDGLMDGTAYDEL 121
Cdd:TIGR00527  86 SPELVNEDPYGDGWLIKVKLSDGEsEVEGLMSAEQYEAT 124
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 6-104 2.24e-22

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 84.50  E-value: 2.24e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2444577466   6 YTPDHEWVQIEADGIVTVGITVHAQDALGDVVFVDLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDDP 85
Cdd:PRK00624    4 YSDYHVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDI 83

                          90
                  ....*....|....*....
gi 2444577466  86 SLANTDPLKAGWFFKVKLN 104
Cdd:PRK00624   84 QPINNAPESEGWFVVVQLD 102
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 22-85 1.34e-07

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 45.51  E-value: 1.34e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2444577466  22 TVGITVHAQdALGDVVFV-DLPAVGTSFASKDVAGVVESVKAAADVYMPVGGEIVEVNEALRDDP 85
Cdd:cd06663     1 TILIPDLAQ-HLGDGTVVkWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKV 64
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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