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Conserved domains on  [gi|2446201946|ref|WP_274076577|]
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glycine cleavage system protein GcvH [Pseudomonas putida]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10793794)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-127 1.14e-66

glycine cleavage system protein H; Provisional


:

Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.92  E-value: 1.14e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   1 MSELRFTV-DHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGSF-DAGAEVSVLESVKAASSIGMPLDGEVLEVNS 78
Cdd:PRK13380   10 PSELRYTIkNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKvEKGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2446201946  79 ELDSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQDQTDA 127
Cdd:PRK13380   90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENIDA 138
 
Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-127 1.14e-66

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.92  E-value: 1.14e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   1 MSELRFTV-DHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGSF-DAGAEVSVLESVKAASSIGMPLDGEVLEVNS 78
Cdd:PRK13380   10 PSELRYTIkNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKvEKGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2446201946  79 ELDSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQDQTDA 127
Cdd:PRK13380   90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENIDA 138
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.11e-59

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 179.55  E-value: 1.11e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   2 SELRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSEL 80
Cdd:COG0509     5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTeVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2446201946  81 DSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQD 123
Cdd:COG0509    85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 4-123 5.48e-43

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 137.46  E-value: 5.48e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   4 LRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELG-SFDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDS 82
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGtKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2446201946  83 TPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQD 123
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKE 121
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-100 3.21e-41

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 131.89  E-value: 3.21e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   6 FTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDSTP 84
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTeVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 2446201946  85 ELVNEDPLGEGWFFRF 100
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 3-123 2.38e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 130.73  E-value: 2.38e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   3 ELRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELD 81
Cdd:TIGR00527   5 DLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAeVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALE 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2446201946  82 STPELVNEDPLGEGWFFRFKPADAA-AVGQLLDQAAYERLIQD 123
Cdd:TIGR00527  85 DSPELVNEDPYGDGWLIKVKLSDGEsEVEGLMSAEQYEATLEH 127
 
Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-127 1.14e-66

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.92  E-value: 1.14e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   1 MSELRFTV-DHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGSF-DAGAEVSVLESVKAASSIGMPLDGEVLEVNS 78
Cdd:PRK13380   10 PSELRYTIkNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKvEKGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2446201946  79 ELDSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQDQTDA 127
Cdd:PRK13380   90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENIDA 138
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.11e-59

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 179.55  E-value: 1.11e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   2 SELRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSEL 80
Cdd:COG0509     5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTeVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2446201946  81 DSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQD 123
Cdd:COG0509    85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-123 4.84e-56

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 170.34  E-value: 4.84e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   1 MSELRFTVDHEWLRTEtDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSE 79
Cdd:PRK01202    5 PADLKYTKSHEWVRVE-GDTATVGITDHAQEQLGDIVFVELPEVGDeVKAGETFGVVESVKAASDIYAPVSGEVVEVNEA 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2446201946  80 LDSTPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQD 123
Cdd:PRK01202   84 LEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 4-123 5.48e-43

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 137.46  E-value: 5.48e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   4 LRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELG-SFDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDS 82
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGtKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2446201946  83 TPELVNEDPLGEGWFFRFKPADAAAVGQLLDQAAYERLIQD 123
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKE 121
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-100 3.21e-41

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 131.89  E-value: 3.21e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   6 FTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDSTP 84
Cdd:cd06848     1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTeVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 2446201946  85 ELVNEDPLGEGWFFRF 100
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 3-123 2.38e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 130.73  E-value: 2.38e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946   3 ELRFTVDHEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGS-FDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELD 81
Cdd:TIGR00527   5 DLRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAeVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALE 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2446201946  82 STPELVNEDPLGEGWFFRFKPADAA-AVGQLLDQAAYERLIQD 123
Cdd:TIGR00527  85 DSPELVNEDPYGDGWLIKVKLSDGEsEVEGLMSAEQYEATLEH 127
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 10-97 5.85e-16

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 68.32  E-value: 5.85e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946  10 HEWLRTETDGTVTVGITAFAQQALGDVVFVQVPELGSF-DAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDSTPELVN 88
Cdd:PRK00624    8 HVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFcKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDIQPIN 87


                  ....*....
gi 2446201946  89 EDPLGEGWF 97
Cdd:PRK00624   88 NAPESEGWF 96
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 22-100 7.98e-07

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 43.58  E-value: 7.98e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2446201946  22 TVGITAFAQQaLGDVVFVQV-PELG-SFDAGAEVSVLESVKAASSIGMPLDGEVLEVNSELDSTPElvnedplGEGWFFR 99
Cdd:cd06663     1 TILIPDLAQH-LGDGTVVKWlKKVGdKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKVE-------GDTPLVK 72


                  .
gi 2446201946 100 F 100
Cdd:cd06663    73 I 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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