NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|2495360635|ref|WP_279655610|]
View 

SIR2 family protein [Agrobacterium sp. GD03642]

Protein Classification

SIR2 family protein( domain architecture ID 1222)

SIR2 family protein similar to NAD-dependent deacetylase that catalyzes NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose

PubMed:  7498786

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
SIR2 super family cl00195
SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which ...
 129-272 6.45e-08

SIR2 superfamily of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer. Also included in this superfamily is a group of uncharacterized Sir2-like proteins which lack certain key catalytic residues and conserved zinc binding cysteines.


The actual alignment was detected with superfamily member pfam13289:

Pssm-ID: 444738  Cd Length: 141  Bit Score: 50.82  E-value: 6.45e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2495360635 129 SHVATLNYDGLLSSAFEMAGvlgdgkvlRDGFIDQKFDTRNLFRHREEGGWYLHLHGCplfVDREKSkpsklaassltrn 208
Cdd:pfam13289   1 PLIITTNYDDLLEKALAGEG--------KPVSAVSFEDLARLAELESGRPLLYKLHGD---LDRPES------------- 56

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2495360635 209 lgqlkhvgrhIVLTHvTHKRTIIAGSEilsTYWEFLKRALdESQEIVIFGYSGNDTHLNQLIAQ 272
Cdd:pfam13289  57 ----------IVLTE-SDYERLLRFKN---PLRRLLRALL-RTRSLLFVGYSFSDPNIRELLRE 105
 
Name Accession Description Interval E-value
SIR2_2 pfam13289
SIR2-like domain; This family of proteins are related to the sirtuins.
 129-272 6.45e-08

SIR2-like domain; This family of proteins are related to the sirtuins.


Pssm-ID: 433090  Cd Length: 141  Bit Score: 50.82  E-value: 6.45e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2495360635 129 SHVATLNYDGLLSSAFEMAGvlgdgkvlRDGFIDQKFDTRNLFRHREEGGWYLHLHGCplfVDREKSkpsklaassltrn 208
Cdd:pfam13289   1 PLIITTNYDDLLEKALAGEG--------KPVSAVSFEDLARLAELESGRPLLYKLHGD---LDRPES------------- 56

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2495360635 209 lgqlkhvgrhIVLTHvTHKRTIIAGSEilsTYWEFLKRALdESQEIVIFGYSGNDTHLNQLIAQ 272
Cdd:pfam13289  57 ----------IVLTE-SDYERLLRFKN---PLRRLLRALL-RTRSLLFVGYSFSDPNIRELLRE 105
 
Name Accession Description Interval E-value
SIR2_2 pfam13289
SIR2-like domain; This family of proteins are related to the sirtuins.
 129-272 6.45e-08

SIR2-like domain; This family of proteins are related to the sirtuins.


Pssm-ID: 433090  Cd Length: 141  Bit Score: 50.82  E-value: 6.45e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2495360635 129 SHVATLNYDGLLSSAFEMAGvlgdgkvlRDGFIDQKFDTRNLFRHREEGGWYLHLHGCplfVDREKSkpsklaassltrn 208
Cdd:pfam13289   1 PLIITTNYDDLLEKALAGEG--------KPVSAVSFEDLARLAELESGRPLLYKLHGD---LDRPES------------- 56

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2495360635 209 lgqlkhvgrhIVLTHvTHKRTIIAGSEilsTYWEFLKRALdESQEIVIFGYSGNDTHLNQLIAQ 272
Cdd:pfam13289  57 ----------IVLTE-SDYERLLRFKN---PLRRLLRALL-RTRSLLFVGYSFSDPNIRELLRE 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH