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Conserved domains on  [gi|2514927407|ref|WP_284561630|]
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nucleotidyltransferase domain-containing protein [Bacillus sp. T2.9-1]

Protein Classification

nucleotidyltransferase domain-containing protein( domain architecture ID 16068202)

nucleotidyltransferase domain-containing protein similar to Methanocaldococcus jannaschii MJ1547, a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and a cognate MNT (minimal nucleotidyltransferase) domain-containing toxin-antitoxin system

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 8-99 1.05e-21

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


:

Pssm-ID: 465859  Cd Length: 93  Bit Score: 82.57  E-value: 1.05e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   8 KKLVDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIA-GREVDLVDIKQSDTVFTMQI 86
Cdd:pfam18765   1 EKIKEILKKNPEIEAAYLFGSRAKGDAREDSDIDLAVLYDEKLDFEELLELASELEDILlLRKVDLVDLNKAPPVLAHQI 80

                          90
                  ....*....|...
gi 2514927407  87 FEQGNPIYIHDEN 99
Cdd:pfam18765  81 LKEGKLLYSRDEE 93
 
Name Accession Description Interval E-value
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 8-99 1.05e-21

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


Pssm-ID: 465859  Cd Length: 93  Bit Score: 82.57  E-value: 1.05e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   8 KKLVDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIA-GREVDLVDIKQSDTVFTMQI 86
Cdd:pfam18765   1 EKIKEILKKNPEIEAAYLFGSRAKGDAREDSDIDLAVLYDEKLDFEELLELASELEDILlLRKVDLVDLNKAPPVLAHQI 80

                          90
                  ....*....|...
gi 2514927407  87 FEQGNPIYIHDEN 99
Cdd:pfam18765  81 LKEGKLLYSRDEE 93
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
1-93 4.61e-15

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


Pssm-ID: 441275  Cd Length: 96  Bit Score: 65.71  E-value: 4.61e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   1 MLKEELQKKL---VDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIAGREVDLVDIKQ 77
Cdd:COG1669     1 MNREEILEILrevIEELAERYGVSRLGLFGSVARGEAREDSDIDLLVEFDEPTSLFDLFELEEELEELLGRKVDLVTLDG 80

                          90
                  ....*....|....*.
gi 2514927407  78 SDTVFTMQIFEQGNPI 93
Cdd:COG1669    81 LSPRLRERILKEGVLL 96
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 4-95 5.07e-14

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 62.82  E-value: 5.07e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   4 EELQKKLVDKII-LETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIAGREVDLVDIKQSDTVF 82
Cdd:cd05403     1 EEILEEILEILReLLGGVEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELARLLEELELLLGRPVDLVVLNALELEL 80

                          90
                  ....*....|...
gi 2514927407  83 TMQIFEQGNPIYI 95
Cdd:cd05403    81 LLRILIEGEGIYL 93
 
Name Accession Description Interval E-value
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 8-99 1.05e-21

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


Pssm-ID: 465859  Cd Length: 93  Bit Score: 82.57  E-value: 1.05e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   8 KKLVDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIA-GREVDLVDIKQSDTVFTMQI 86
Cdd:pfam18765   1 EKIKEILKKNPEIEAAYLFGSRAKGDAREDSDIDLAVLYDEKLDFEELLELASELEDILlLRKVDLVDLNKAPPVLAHQI 80

                          90
                  ....*....|...
gi 2514927407  87 FEQGNPIYIHDEN 99
Cdd:pfam18765  81 LKEGKLLYSRDEE 93
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
1-93 4.61e-15

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


Pssm-ID: 441275  Cd Length: 96  Bit Score: 65.71  E-value: 4.61e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   1 MLKEELQKKL---VDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIAGREVDLVDIKQ 77
Cdd:COG1669     1 MNREEILEILrevIEELAERYGVSRLGLFGSVARGEAREDSDIDLLVEFDEPTSLFDLFELEEELEELLGRKVDLVTLDG 80

                          90
                  ....*....|....*.
gi 2514927407  78 SDTVFTMQIFEQGNPI 93
Cdd:COG1669    81 LSPRLRERILKEGVLL 96
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 4-95 5.07e-14

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 62.82  E-value: 5.07e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   4 EELQKKLVDKII-LETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIAGREVDLVDIKQSDTVF 82
Cdd:cd05403     1 EEILEEILEILReLLGGVEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELARLLEELELLLGRPVDLVVLNALELEL 80

                          90
                  ....*....|...
gi 2514927407  83 TMQIFEQGNPIYI 95
Cdd:cd05403    81 LLRILIEGEGIYL 93
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
4-90 5.34e-14

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 63.12  E-value: 5.34e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   4 EELQKKLVDKIILETNPAFVILFGSFAKGTTYDASDIDLAYFSDKKLSSYERFMLASELAAIAGREVDLVDIKQSD---T 80
Cdd:COG1708     6 RELLEEIVEALRRGPEVAAVYLFGSYARGDARPDSDIDLLVVVDDPPLPDERLELLADLLRELGLPVDLVVLTPAElelR 85

                          90
                  ....*....|
gi 2514927407  81 VFTMQIFEQG 90
Cdd:COG1708    86 LFLREILREG 95
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 2-73 1.08e-04

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 38.55  E-value: 1.08e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2514927407   2 LKEELQKKLVDkiiletnpAFVILFGSFAKGTTYDASDIDLAYFSD---KKLSSYERFMLASELAAIAGREVDLV 73
Cdd:pfam01909   4 LREILKELFPV--------AEVVLFGSYARGTALPGSDIDLLVVFPepvEEERLLKLAKIIKELEELLGLEVDLV 70
NT_2-5OAS_ClassI-CCAase cd05400
Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class ...
 2-90 9.36e-04

Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class I CCA-adding enzyme; In vertebrates, 2-5OASs are induced by interferon during the innate immune response to protect against RNA virus infections. In the presence of an RNA activator, 2-5OASs catalyze the oligomerization of ATP into 2-5A. 2-5A activates endoribonuclease L, which leads to degradation of the viral RNA. 2-5OASs are also implicated in cell growth control, differentiation, and apoptosis. This family includes human OAS1, -2, -3, and OASL. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This class I group includes the archaeal Sulfolobus shibatae and Archeoglobus fulgidus CCA-adding enzymes. It belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this family.


Pssm-ID: 143390 [Multi-domain]  Cd Length: 143  Bit Score: 36.99  E-value: 9.36e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2514927407   2 LKEELQKKLVDKIILETNpafVILFGSFAKGTT-YDASDIDLAYF--SDKKLSSYERFMLASEL-AAIAGREVDLVDIKQ 77
Cdd:cd05400    12 IREALKESLSELAGRVAE---VFLQGSYARGTAlRGDSDIDLVVVlpDDTSFAEYGPAELLDELgEALKEYYGANEEVKA 88

                          90
                  ....*....|...
gi 2514927407  78 SDTVFTMQIFEQG 90
Cdd:cd05400    89 QHRSVTVKFKGQG 101
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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