|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07726 |
PRK07726 |
DNA topoisomerase 3; |
1-605 |
0e+00 |
|
DNA topoisomerase 3;
Pssm-ID: 236078 [Multi-domain] Cd Length: 658 Bit Score: 758.54 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDGFITcKDGSVVTWCFGHLLEQAEPDAYlpdDvPRTKKgskvWRFEDLPIYPKNW 80
Cdd:PRK07726 1 MRLFIAEKPSVGRDIADVLKPHKKGDGYIE-GNGYIVTWAIGHLLELAEPEAY---D-ERYKR----WRLEDLPIIPEKW 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 KLLPKndKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNETF 160
Cdd:PRK07726 72 KLVVK--KKTAKQFNVVKKLLKQATEIVIATDADREGELIAREILDYCGVRKPIKRLWISSLTDKAIKRAFANLKPGKET 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 161 DGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGGGKPFT 240
Cdd:PRK07726 150 IPLYYSALARSRADWLVGINMTRAYTLLGRKAGYNGVLSVGRVQTPTLALVVRRDEEIENFVPKPYWEVEAHLTPGERFT 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 241 AVWEPeeSQAGIDEQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQ 320
Cdd:PRK07726 230 AKWQP--SEPYQDEEGRLLDRPLAEQVVARIQG-QPAKVTEVETKRKKEYAPLLYDLSELQIDANKRFGLSAKETLDIAQ 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 321 SLYETHKATSYPRTDCSFLPESQYADAKNVLAAIAKTMPPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQAADGSKF 400
Cdd:PRK07726 307 SLYETHKLITYPRTDSRYLPEDMVATLPEVLNAISKVDPYLLLAPPVLDPSIRSRAWNDKKVTAHHAIIPTEQPPNLSKL 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 401 SDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDkaDDEGQALPELKSGLQLP 480
Cdd:PRK07726 387 SEDERKVYDLIARRYLAQFLPPAEYDKTTIELEIAGGTFIAKGKQVVEAGWKALLGKKEED--EEKEQPLPVLAKGDELK 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 481 VSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEGDGIGTPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:PRK07726 465 VEKGEVKEGQTQPPKRFTEGTLLSAMENIARFVQDKELKKTLKETDGLGTEATRAGIIEKLFKRGYLEKKGKYIHATDKG 544
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 2527498429 561 LHLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV 605
Cdd:PRK07726 545 KQLIDALPEELTSPDMTAKWEQALDQISEGQLSYQDFMQPLKQWL 589
|
|
| topB |
TIGR01056 |
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase ... |
1-611 |
0e+00 |
|
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase III from bacteria and its equivalents encoded on plasmids. The gene is designated topB if found in the bacterial chromosome, traE on conjugative plasmid RP4, etc. These enzymes are involved in the control of DNA topology. DNA topoisomerase III belongs to the type I topoisomerases, which are ATP-independent. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273419 [Multi-domain] Cd Length: 660 Bit Score: 595.67 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDGFITCKDGSVVTWCFGHLLEQAEPDAYlpddvprtKKGSKVWRFEDLPIYPKNW 80
Cdd:TIGR01056 1 MTLVLCEKPSQARDLATVLAKKKKGNGYLEIGVGGFVTWAVGHLVELAEPEEY--------DEKYKNWRTYDLPLEPEDW 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 KLLPKNDkgVKKQLATIGKLLK--KASLVVHAGDPDREEQLLVDEVLEHFRYTG--RVQRFWFSAQDSASIRKGLTNLRD 156
Cdd:TIGR01056 73 QLVVSDK--TKKQFNVIKRILKenKVDEVVIATDPDREGELIAREILDYLKVTDkvTIKRLWISSLVDSSIRKAFKNLRP 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 157 NETFDGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARL-GG 235
Cdd:TIGR01056 151 KSETEGLYKSGVARARADWLVGINLTRAFTKLGREAGNDGVLSVGRVQTPTLAMVVKRENEIKNFVGKPFYEVSATInKD 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 236 GKPFTAVWEPEEsqagiDEQKRLVDRCIAAALQQRLKAVgQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKT 315
Cdd:TIGR01056 231 EQEFTTEWQPYK-----DEEERELHEFLAENVVTDLTQK-PALVTDIEKERKKTSAPLFYDLSALQEDANKRFGISAKRT 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 316 LNLCQSLYETHKATSYPRTDCSFLPESQYADAKNVLAAIaKTMPPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQAA 395
Cdd:TIGR01056 305 LDIAQKLYETHKLITYPRTDSRYLPEDEKEMLLEVLDAL-KVITPALLPIKKRDELTNNRLWNDKKIEDHHAIIPTGNDF 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 396 DGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDKaDDEGQALPELKS 475
Cdd:TIGR01056 384 NLSDLSEEERNVYKLIAQNYLMQFMPKEEYETTTIEIAIGKLMFEAKGKILQDNGWKALLGKQEEDE-ETEDTTLPAFQK 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 476 GLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEGDGIGTPATRAAIIAELKRKKYLEVKGKKIV 555
Cdd:TIGR01056 463 GDELDVETLELLEKQTKPPARYTEGTLLSAMTNPAAFVQDKGLKKTLKETKGLGTEATRADIIENLFKRGFIQKKKNKIY 542
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 2527498429 556 ATELGLHLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV---INELRR 611
Cdd:TIGR01056 543 ITKNGKLLCLLLPELLTKPDLTAQWEQYLNGISAGEKDDDDFINTINEMIkqtINEEKK 601
|
|
| TopA |
COG0550 |
DNA topoisomerase IA [Replication, recombination and repair]; |
1-600 |
9.97e-148 |
|
DNA topoisomerase IA [Replication, recombination and repair];
Pssm-ID: 440316 [Multi-domain] Cd Length: 541 Bit Score: 437.60 E-value: 9.97e-148
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGcvtrgDGFItckdgsvVTWCFGHLLEQAEPDAYLPDDVprtkkgskvwRFEdlPIYpknw 80
Cdd:COG0550 1 KKLVIVESPAKAKTIAKYLG-----KDYV-------VTASVGHLRDLPKPELDVDVEN----------DFE--PKY---- 52
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 kllpKNDKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNEtf 160
Cdd:COG0550 53 ----EVIPGKKKQVKELKKLAKKADEVILATDPDREGEAIAWHLLELLGDKKPVKRVVFNEITKKAIKEAFKNPRDID-- 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 161 dgMRL--AALGRSRADWLLGMNLSRAYTLAQQAQgkkelIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLG-GGK 237
Cdd:COG0550 127 --MNLvdAQQARRILDRLVGYNLSPLLWKKVKRG-----LSAGRVQSPALRLIVEREREIEAFVPEEYWTIEATFKtGGG 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 238 PFTAVWEPEEsqagideQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLN 317
Cdd:COG0550 200 EFTAKLVKID-------GKKILDEEEAEAILAALKG-KTFTVTSVEKKPKKRNPPPPFTTSTLQQEASRKLGFSAKKTMS 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 318 LCQSLYEThKATSYPRTDCSFLPESQYADAKNVLAAI--AKTMPPLAGLVAKcdcsiqsptwNDKKITAHHGIIPTQQAA 395
Cdd:COG0550 272 IAQRLYEV-GLITYMRTDSVRLSEDAVAAAREYIEALygKEYLPEKPRVYKT----------SKNAQEAHEAIRPTGVTR 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 396 DGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDKADDEGQALPELKS 475
Cdd:COG0550 341 TPESLSGDEFKLYDLIWKRFLASQMPPAEYETTTVTIEVGGEEFRASGKVIKFPGFLKVYGEGKDDEEEEEEKRLPPLKE 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 476 GLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIV 555
Cdd:COG0550 421 GEKLKLKKIEPEQHFTKPPPRYTEASLVKAMEEL-----------------GIGRPSTYASIIETLQDRGYVEREGKRLV 483
|
570 580 590 600
....*....|....*....|....*....|....*....|....*....
gi 2527498429 556 ATELGLHLLQVV----PDVMkNPVLTALFERILREVEAGNVPLDVFIEK 600
Cdd:COG0550 484 PTELGFIVIDLLeehfPDLV-DYEFTAKMEEKLDEIAEGKLDWKDVLEE 531
|
|
| Topoisom_bac |
pfam01131 |
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes ... |
158-590 |
8.60e-120 |
|
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes preferentially relax negatively supercoiled DNA, from a 5' phospho- tyrosine linkage in the enzyme-DNA covalent intermediate and has high affinity for single stranded DNA.
Pssm-ID: 426069 [Multi-domain] Cd Length: 410 Bit Score: 361.56 E-value: 8.60e-120
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 158 ETFDGMRLAALGRSRADWLLGMNLSRAYTLAQqaqGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGGGK 237
Cdd:pfam01131 1 EIDENLVDAQLARRELDWLIGFNLSRLLTLKL---KGNKLLSAGRVQTPTLALIVEREREIENFVPEPYWTIEATLKDGG 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 238 PFTAVWEpeesqagiDEQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLN 317
Cdd:pfam01131 78 GVFANAK--------WKRGRIFDKEEAEAILEKLKK-GTAVVTSVESKEKKKPPPLPFNTTTLQKEASRKLGFSAKKTMS 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 318 LCQSLYETHKATSYPRTDCSFLPESqyADAKNVLAAIAKTMPPLAGLVAKCDCSIQSP-TWNDKKITAHHGIIPTQQAAD 396
Cdd:pfam01131 149 IAQKLYEKHGLITYPRTDSTYLSDD--ATLKAILEYLTKNYGKYLPYAPRLLKKTKGKlTVGNAKVTAHEAIRPTGDIPV 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 397 GSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVtsaaDEDKADDEGQALPELKSG 476
Cdd:pfam01131 227 LPELSDDERKLYELIVRRFLASQMPPAVYETTTVVLEVGGEEFRASGKVILEDGWLKV----YPYEKKEDEEELPELKEG 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 477 LQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIVA 556
Cdd:pfam01131 303 EELKVTQVEVEEKKTKPPPRYTEASLIKAMEKA-----------------GIGTPATRATIIETLLERGYVERKKKSLVP 365
|
410 420 430
....*....|....*....|....*....|....*..
gi 2527498429 557 TELGL---HLLQVVPDVMkNPVLTALFERILREVEAG 590
Cdd:pfam01131 366 TELGKalvEALEKFPDLV-DPELTAELEEKLDEIAEG 401
|
|
| TOP1Ac |
cd00186 |
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA ... |
166-605 |
1.58e-93 |
|
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen, upon cleavage of the scissile strand.
Pssm-ID: 238110 [Multi-domain] Cd Length: 381 Bit Score: 292.59 E-value: 1.58e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 166 AALGRSRADWLLGMNLSRAYTLAQQaqgKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKarlgggkpftavwep 245
Cdd:cd00186 6 AQLARRILDRLVGFNLSRLLTKKLR---RKGVLSAGRVQSPTLGLIVEREREIKAFVPEDYWEIK--------------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 246 eesqagideqkrlvdrciaaalqqrlkavgQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQSLYET 325
Cdd:cd00186 68 ------------------------------EAVVVSVEKKEKKKNPPPPFTTSTLQQEASSKLGFSAKKTMQIAQKLYEA 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 326 HKaTSYPRTDCSFLPESQYADAKNVLAAIAktmpPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQA---ADGSKFSD 402
Cdd:cd00186 118 GL-ITYPRTDSTRLSEEAILEAREYIQAIY----GKEYLYPAPLLGRRNPKRGKKEQGAHEAIRPTKVAptpELEANLSE 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 403 DERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTsaaDEDKADDEgQALPELKSGLQLPVS 482
Cdd:cd00186 193 DEFKLYELIWRRFLASQMADAKYEETTVTLEIGGEKFKASGKVLLEDGWLEVY---PEEKDDEE-EEPPPLKEGDELKLE 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 483 GIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIVATELGL- 561
Cdd:cd00186 269 EVELEEKETQPPPRYTEASLIKLMEKR-----------------GIGRPSTYASIIETLLDRGYVEKEKKKLIPTELGFa 331
|
410 420 430 440
....*....|....*....|....*....|....*....|....*.
gi 2527498429 562 --HLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV 605
Cdd:cd00186 332 viELLEKHFPELVDPEFTAKLEEKLDEIAEGKKDYQEVLEEFYEEF 377
|
|
| TOP1Ac |
smart00437 |
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, ... |
284-560 |
1.92e-66 |
|
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase alpha subunit
Pssm-ID: 214663 [Multi-domain] Cd Length: 259 Bit Score: 217.88 E-value: 1.92e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 284 RTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQSLYEtHKATSYPRTDCSFLPESQYADAKNvlaAIAKTMPPLAG 363
Cdd:smart00437 2 EKEKKKNPPPPFTTSTLQQEASRKLGFSAKKTMQIAQKLYE-KGLITYPRTDSTRLSEEAVLEARN---YIQKLYGKEYL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 364 LVAKCDCSIQSPTWNDKKITAHHGIIPTQQA---ADGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFS 440
Cdd:smart00437 78 DLAVVLLKPRKPRKGKKAQGAHEAIRPTKPIftpELEKELSEDEFKLYELIWRRFLASQMPDAKYEETKVTIKIGGEKFK 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 441 AKGRLVTKPGWKVVTSAADedkaDDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENihqavndpqskk 520
Cdd:smart00437 158 AKGKTLLFDGWLKVYPEEK----KEEEIELPTLKKGDELKVEEVEVEEKETKPPARYTEASLIKLMEK------------ 221
|
250 260 270 280
....*....|....*....|....*....|....*....|
gi 2527498429 521 flkegDGIGTPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:smart00437 222 -----RGIGRPSTYASIIETLLDRGYVTKKKKKLIPTELG 256
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07726 |
PRK07726 |
DNA topoisomerase 3; |
1-605 |
0e+00 |
|
DNA topoisomerase 3;
Pssm-ID: 236078 [Multi-domain] Cd Length: 658 Bit Score: 758.54 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDGFITcKDGSVVTWCFGHLLEQAEPDAYlpdDvPRTKKgskvWRFEDLPIYPKNW 80
Cdd:PRK07726 1 MRLFIAEKPSVGRDIADVLKPHKKGDGYIE-GNGYIVTWAIGHLLELAEPEAY---D-ERYKR----WRLEDLPIIPEKW 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 KLLPKndKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNETF 160
Cdd:PRK07726 72 KLVVK--KKTAKQFNVVKKLLKQATEIVIATDADREGELIAREILDYCGVRKPIKRLWISSLTDKAIKRAFANLKPGKET 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 161 DGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGGGKPFT 240
Cdd:PRK07726 150 IPLYYSALARSRADWLVGINMTRAYTLLGRKAGYNGVLSVGRVQTPTLALVVRRDEEIENFVPKPYWEVEAHLTPGERFT 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 241 AVWEPeeSQAGIDEQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQ 320
Cdd:PRK07726 230 AKWQP--SEPYQDEEGRLLDRPLAEQVVARIQG-QPAKVTEVETKRKKEYAPLLYDLSELQIDANKRFGLSAKETLDIAQ 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 321 SLYETHKATSYPRTDCSFLPESQYADAKNVLAAIAKTMPPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQAADGSKF 400
Cdd:PRK07726 307 SLYETHKLITYPRTDSRYLPEDMVATLPEVLNAISKVDPYLLLAPPVLDPSIRSRAWNDKKVTAHHAIIPTEQPPNLSKL 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 401 SDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDkaDDEGQALPELKSGLQLP 480
Cdd:PRK07726 387 SEDERKVYDLIARRYLAQFLPPAEYDKTTIELEIAGGTFIAKGKQVVEAGWKALLGKKEED--EEKEQPLPVLAKGDELK 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 481 VSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEGDGIGTPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:PRK07726 465 VEKGEVKEGQTQPPKRFTEGTLLSAMENIARFVQDKELKKTLKETDGLGTEATRAGIIEKLFKRGYLEKKGKYIHATDKG 544
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 2527498429 561 LHLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV 605
Cdd:PRK07726 545 KQLIDALPEELTSPDMTAKWEQALDQISEGQLSYQDFMQPLKQWL 589
|
|
| topB |
TIGR01056 |
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase ... |
1-611 |
0e+00 |
|
DNA topoisomerase III, bacteria and conjugative plasmid; This model describes topoisomerase III from bacteria and its equivalents encoded on plasmids. The gene is designated topB if found in the bacterial chromosome, traE on conjugative plasmid RP4, etc. These enzymes are involved in the control of DNA topology. DNA topoisomerase III belongs to the type I topoisomerases, which are ATP-independent. [DNA metabolism, DNA replication, recombination, and repair]
Pssm-ID: 273419 [Multi-domain] Cd Length: 660 Bit Score: 595.67 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDGFITCKDGSVVTWCFGHLLEQAEPDAYlpddvprtKKGSKVWRFEDLPIYPKNW 80
Cdd:TIGR01056 1 MTLVLCEKPSQARDLATVLAKKKKGNGYLEIGVGGFVTWAVGHLVELAEPEEY--------DEKYKNWRTYDLPLEPEDW 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 KLLPKNDkgVKKQLATIGKLLK--KASLVVHAGDPDREEQLLVDEVLEHFRYTG--RVQRFWFSAQDSASIRKGLTNLRD 156
Cdd:TIGR01056 73 QLVVSDK--TKKQFNVIKRILKenKVDEVVIATDPDREGELIAREILDYLKVTDkvTIKRLWISSLVDSSIRKAFKNLRP 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 157 NETFDGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARL-GG 235
Cdd:TIGR01056 151 KSETEGLYKSGVARARADWLVGINLTRAFTKLGREAGNDGVLSVGRVQTPTLAMVVKRENEIKNFVGKPFYEVSATInKD 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 236 GKPFTAVWEPEEsqagiDEQKRLVDRCIAAALQQRLKAVgQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKT 315
Cdd:TIGR01056 231 EQEFTTEWQPYK-----DEEERELHEFLAENVVTDLTQK-PALVTDIEKERKKTSAPLFYDLSALQEDANKRFGISAKRT 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 316 LNLCQSLYETHKATSYPRTDCSFLPESQYADAKNVLAAIaKTMPPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQAA 395
Cdd:TIGR01056 305 LDIAQKLYETHKLITYPRTDSRYLPEDEKEMLLEVLDAL-KVITPALLPIKKRDELTNNRLWNDKKIEDHHAIIPTGNDF 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 396 DGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDKaDDEGQALPELKS 475
Cdd:TIGR01056 384 NLSDLSEEERNVYKLIAQNYLMQFMPKEEYETTTIEIAIGKLMFEAKGKILQDNGWKALLGKQEEDE-ETEDTTLPAFQK 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 476 GLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEGDGIGTPATRAAIIAELKRKKYLEVKGKKIV 555
Cdd:TIGR01056 463 GDELDVETLELLEKQTKPPARYTEGTLLSAMTNPAAFVQDKGLKKTLKETKGLGTEATRADIIENLFKRGFIQKKKNKIY 542
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 2527498429 556 ATELGLHLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV---INELRR 611
Cdd:TIGR01056 543 ITKNGKLLCLLLPELLTKPDLTAQWEQYLNGISAGEKDDDDFINTINEMIkqtINEEKK 601
|
|
| TopA |
COG0550 |
DNA topoisomerase IA [Replication, recombination and repair]; |
1-600 |
9.97e-148 |
|
DNA topoisomerase IA [Replication, recombination and repair];
Pssm-ID: 440316 [Multi-domain] Cd Length: 541 Bit Score: 437.60 E-value: 9.97e-148
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGcvtrgDGFItckdgsvVTWCFGHLLEQAEPDAYLPDDVprtkkgskvwRFEdlPIYpknw 80
Cdd:COG0550 1 KKLVIVESPAKAKTIAKYLG-----KDYV-------VTASVGHLRDLPKPELDVDVEN----------DFE--PKY---- 52
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 81 kllpKNDKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNEtf 160
Cdd:COG0550 53 ----EVIPGKKKQVKELKKLAKKADEVILATDPDREGEAIAWHLLELLGDKKPVKRVVFNEITKKAIKEAFKNPRDID-- 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 161 dgMRL--AALGRSRADWLLGMNLSRAYTLAQQAQgkkelIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLG-GGK 237
Cdd:COG0550 127 --MNLvdAQQARRILDRLVGYNLSPLLWKKVKRG-----LSAGRVQSPALRLIVEREREIEAFVPEEYWTIEATFKtGGG 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 238 PFTAVWEPEEsqagideQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLN 317
Cdd:COG0550 200 EFTAKLVKID-------GKKILDEEEAEAILAALKG-KTFTVTSVEKKPKKRNPPPPFTTSTLQQEASRKLGFSAKKTMS 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 318 LCQSLYEThKATSYPRTDCSFLPESQYADAKNVLAAI--AKTMPPLAGLVAKcdcsiqsptwNDKKITAHHGIIPTQQAA 395
Cdd:COG0550 272 IAQRLYEV-GLITYMRTDSVRLSEDAVAAAREYIEALygKEYLPEKPRVYKT----------SKNAQEAHEAIRPTGVTR 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 396 DGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAADEDKADDEGQALPELKS 475
Cdd:COG0550 341 TPESLSGDEFKLYDLIWKRFLASQMPPAEYETTTVTIEVGGEEFRASGKVIKFPGFLKVYGEGKDDEEEEEEKRLPPLKE 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 476 GLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIV 555
Cdd:COG0550 421 GEKLKLKKIEPEQHFTKPPPRYTEASLVKAMEEL-----------------GIGRPSTYASIIETLQDRGYVEREGKRLV 483
|
570 580 590 600
....*....|....*....|....*....|....*....|....*....
gi 2527498429 556 ATELGLHLLQVV----PDVMkNPVLTALFERILREVEAGNVPLDVFIEK 600
Cdd:COG0550 484 PTELGFIVIDLLeehfPDLV-DYEFTAKMEEKLDEIAEGKLDWKDVLEE 531
|
|
| Topoisom_bac |
pfam01131 |
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes ... |
158-590 |
8.60e-120 |
|
DNA topoisomerase; This subfamily of topoisomerase is divided on the basis that these enzymes preferentially relax negatively supercoiled DNA, from a 5' phospho- tyrosine linkage in the enzyme-DNA covalent intermediate and has high affinity for single stranded DNA.
Pssm-ID: 426069 [Multi-domain] Cd Length: 410 Bit Score: 361.56 E-value: 8.60e-120
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 158 ETFDGMRLAALGRSRADWLLGMNLSRAYTLAQqaqGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGGGK 237
Cdd:pfam01131 1 EIDENLVDAQLARRELDWLIGFNLSRLLTLKL---KGNKLLSAGRVQTPTLALIVEREREIENFVPEPYWTIEATLKDGG 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 238 PFTAVWEpeesqagiDEQKRLVDRCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLN 317
Cdd:pfam01131 78 GVFANAK--------WKRGRIFDKEEAEAILEKLKK-GTAVVTSVESKEKKKPPPLPFNTTTLQKEASRKLGFSAKKTMS 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 318 LCQSLYETHKATSYPRTDCSFLPESqyADAKNVLAAIAKTMPPLAGLVAKCDCSIQSP-TWNDKKITAHHGIIPTQQAAD 396
Cdd:pfam01131 149 IAQKLYEKHGLITYPRTDSTYLSDD--ATLKAILEYLTKNYGKYLPYAPRLLKKTKGKlTVGNAKVTAHEAIRPTGDIPV 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 397 GSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVtsaaDEDKADDEGQALPELKSG 476
Cdd:pfam01131 227 LPELSDDERKLYELIVRRFLASQMPPAVYETTTVVLEVGGEEFRASGKVILEDGWLKV----YPYEKKEDEEELPELKEG 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 477 LQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIVA 556
Cdd:pfam01131 303 EELKVTQVEVEEKKTKPPPRYTEASLIKAMEKA-----------------GIGTPATRATIIETLLERGYVERKKKSLVP 365
|
410 420 430
....*....|....*....|....*....|....*..
gi 2527498429 557 TELGL---HLLQVVPDVMkNPVLTALFERILREVEAG 590
Cdd:pfam01131 366 TELGKalvEALEKFPDLV-DPELTAELEEKLDEIAEG 401
|
|
| PRK08173 |
PRK08173 |
DNA topoisomerase III; Validated |
3-604 |
1.90e-108 |
|
DNA topoisomerase III; Validated
Pssm-ID: 236172 [Multi-domain] Cd Length: 862 Bit Score: 345.88 E-value: 1.90e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 3 LFIAEKPSVAKAIVAELGCVTRGDGFITcKDGSVVTWCFGHLLEQAEPDAYlpdDVPRTKkgskvWRFEDLPIYPKNWKL 82
Cdd:PRK08173 5 LIIAEKPSVANDIARALGGFTKHDEYFE-SDEYVLSSAVGHLLEIAAPEEY---EVKRGK-----WSFAHLPVIPPHFDL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 83 LPKnDKgVKKQLATIGKLLKKASL--VVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNETF 160
Cdd:PRK08173 76 NPI-AK-TESRLKVLTKLIKRKDVtrLINACDAGREGELIFRLIAQHAKAKKPVKRLWLQSMTPQAIRDGFANLRSDEDM 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 161 DGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGG----- 235
Cdd:PRK08173 154 QPLADAARCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVRAEFVAaagfy 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 236 -GKPFTAVWEPEESQAGiDEQKRLVDRciAAALQQRLKAVGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEK 314
Cdd:PRK08173 234 eGRWFDPKFKKDEFDPE-KRASRLWSE--AAAEAIVAACRGKPGTVTEESKPSTQLSPLLFDLTSLQREANGRFGFSAKN 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 315 TLNLCQSLYETHKATSYPRTDCSFLPESQYADAKNVLAAIAKTMPPLAglVAKcdcSIQSPTW--------NDKKITAHH 386
Cdd:PRK08173 311 TLGLAQALYEKHKVLTYPRTDSRALPEDYLGTVKQTLEMLKESNNYLP--HAK---QILDKGWvkpnkrifDNSKISDHF 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 387 GIIPTQQAADGskFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTSAAdedkADDE 466
Cdd:PRK08173 386 AIIPTLQAPKS--LSEPEQKLYDLVVKRFLAVFFPAAEFLVTTRITEVAGHHFKTEGKVLVNPGWLAVYGKE----AQGA 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 467 GQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEgDGIGTPATRAAIIAELKRKKY 546
Cdd:PRK08173 460 DANLVPVQKGEKVKTDKIEAVALTTKPPARYNEATLLSAMEGAGKLVEDDELREAMAE-KGLGTPATRAAIIEGLLGEKY 538
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2527498429 547 LEVKGKKIVATELGLHLLQV-----VPDVMKnPVLTALFERILREVEAGNVPLDVFIEKQKQL 604
Cdd:PRK08173 539 LVREGRELIPTAKAFQLMTLlrglgVEELTS-PELTGEWEYKLSQIERGKLSRDAFMQEIAQM 600
|
|
| TOP1Ac |
cd00186 |
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA ... |
166-605 |
1.58e-93 |
|
DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen, upon cleavage of the scissile strand.
Pssm-ID: 238110 [Multi-domain] Cd Length: 381 Bit Score: 292.59 E-value: 1.58e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 166 AALGRSRADWLLGMNLSRAYTLAQQaqgKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKarlgggkpftavwep 245
Cdd:cd00186 6 AQLARRILDRLVGFNLSRLLTKKLR---RKGVLSAGRVQSPTLGLIVEREREIKAFVPEDYWEIK--------------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 246 eesqagideqkrlvdrciaaalqqrlkavgQATVVRCSRTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQSLYET 325
Cdd:cd00186 68 ------------------------------EAVVVSVEKKEKKKNPPPPFTTSTLQQEASSKLGFSAKKTMQIAQKLYEA 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 326 HKaTSYPRTDCSFLPESQYADAKNVLAAIAktmpPLAGLVAKCDCSIQSPTWNDKKITAHHGIIPTQQA---ADGSKFSD 402
Cdd:cd00186 118 GL-ITYPRTDSTRLSEEAILEAREYIQAIY----GKEYLYPAPLLGRRNPKRGKKEQGAHEAIRPTKVAptpELEANLSE 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 403 DERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVTsaaDEDKADDEgQALPELKSGLQLPVS 482
Cdd:cd00186 193 DEFKLYELIWRRFLASQMADAKYEETTVTLEIGGEKFKASGKVLLEDGWLEVY---PEEKDDEE-EEPPPLKEGDELKLE 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 483 GIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIVATELGL- 561
Cdd:cd00186 269 EVELEEKETQPPPRYTEASLIKLMEKR-----------------GIGRPSTYASIIETLLDRGYVEKEKKKLIPTELGFa 331
|
410 420 430 440
....*....|....*....|....*....|....*....|....*.
gi 2527498429 562 --HLLQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQLV 605
Cdd:cd00186 332 viELLEKHFPELVDPEFTAKLEEKLDEIAEGKKDYQEVLEEFYEEF 377
|
|
| PRK14724 |
PRK14724 |
DNA topoisomerase III; Provisional |
3-600 |
4.74e-93 |
|
DNA topoisomerase III; Provisional
Pssm-ID: 237803 [Multi-domain] Cd Length: 987 Bit Score: 307.65 E-value: 4.74e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 3 LFIAEKPSVAKAIVAEL----GCVTRGDGFITcKDGSVVTWCFGHLLEQAEPDAYlpdDVPRTKkgskvWRFEDLPIYPK 78
Cdd:PRK14724 4 LVIAEKPSVAQDIVRALtpvaGKFEKHDEHFE-SDSYVVTSAVGHLVEIQAPEEF---DVKRGK-----WSFANLPVIPP 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 79 NWKLLPKnDKgVKKQLATIGKLLKKASL--VVHAGDPDREEQLLVdEVLEHFRYTGR------VQRFWFSAQDSASIRKG 150
Cdd:PRK14724 75 YFDLKPV-DK-TKTRLNAVVKLAKRKDVteLVNACDAGREGELIF-RLIEQYAGGAKgglgkpVKRLWLQSMTPQAIRDG 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 151 LTNLRDNETFDGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIK 230
Cdd:PRK14724 152 FDNLRSDAQMQGLASAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSLVVEREEKIRKFVSRDYWEIH 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 231 ARLGG------GKPFTAVWEPEESQAGIDEQKRLVDR---CIAAALQqrlkavGQATVVRCSRTPKKIAQPKAFSLADIQ 301
Cdd:PRK14724 232 AGFHAeageylGKWFDPQWKKASDDPEARADRVWSERearAIADAVR------GKAATVTEESKPTTQASPLLFDLTSLQ 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 302 LGASNQFGFSAEKTLNLCQSLYETHKATSYPRTDCSFLPESQYADAKNVLAAIAKT-MPPLAGLVAKC-DCSIQSPT--- 376
Cdd:PRK14724 306 REANGKFGFSAKTTLALAQSLYERHKALTYPRTDSRALPEDYLPVAKQTFEMLATSgMRHLAPFAQQAlDGNYVRPSkri 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 377 WNDKKITAHHGIIPTQQAADGskFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVV-- 454
Cdd:PRK14724 386 FDNSKVSDHFAIIPTTQAPSG--LSEAEQKLYDLVVRRFMAVFFPSAEYQVTTRISQVVGHSFKTEGKVLVKPGWLAIyg 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 455 TSAADED---KADDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIHQAVNDPQSKKFLKEgDGIGTP 531
Cdd:PRK14724 464 KEAANEVedaKDGDKGQPLVPVKPGEMVRTEFAEAKGLKTKPPARYSEATLLGAMESAGKQIDDDELREAMQE-KGLGTP 542
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2527498429 532 ATRAAIIAELKRKKYLEVKGKKIVATELGLHLLQVVPDV----MKNPVLTALFERILREVEAGNVPLDVFIEK 600
Cdd:PRK14724 543 ATRAAIIEGLLTEKYMLREGRELIPTAKAFQLMTLLRGLgveeLSRAELTGEWEYKLAQMEKGQLSREAFMQQ 615
|
|
| PRK07219 |
PRK07219 |
DNA topoisomerase I; Validated |
1-612 |
6.87e-81 |
|
DNA topoisomerase I; Validated
Pssm-ID: 235971 [Multi-domain] Cd Length: 822 Bit Score: 271.87 E-value: 6.87e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGC----VTRGDG-----FITCKDGSVVTWCFGHLLEQAEPDaylpddvprtkkgskvwRFE 71
Cdd:PRK07219 1 TELIIAEKNNAARRIADILSGgkakKKRVNGvpyyeFERKGEKWIVIGLSGHIVTVDFPE-----------------EYG 63
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 72 DLPIYPKNWKLLPKNDKGVKKQ--LATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGR--VQRFWFSAQDSASI 147
Cdd:PRK07219 64 DWRDVDPAELIDADPVKKITKQnyINALKKLAKDADEIIIATDYDREGELIGKEAYHILREVCQvpVKRARFSSLTKKEI 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 148 RKGLTNLrDNETFDgmrLAALGRSRA--DWLLGMNLSRAYTLAQQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVP 225
Cdd:PRK07219 144 RKAFENP-DEIDFN---LADAGEARQiiDLYWGAALTRFLSLSVRQLGRWDFLSVGRVQTPTLAFIVDREREIRAFDPED 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 226 YFVIKARLggGKPFTAVWEPEEsqaGIDEQKRLVDRCIAAALQQRLKAVGQATVVRCSRTPKKIAQPKAFSLADIQLGAS 305
Cdd:PRK07219 220 YWKIEALL--DKEAQYFYRDLI---GGHEAEKFWDEEEAEEIYEKLKGAKEATVSSVKKRERTISPPAPFNTTEFLREAS 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 306 NQFGFSAEKTLNLCQSLYEthKA-TSYPRTDCSFLPESQyaDAKNVLAAIA--KTMPPLAGLVAKCDcsIQSPTWNDKKI 382
Cdd:PRK07219 295 KIFGISPKRAMEIAEKLYT--AGyISYPRTDNTVYPDDL--DPKELLKKLSkkKEYGPYAESILEQE--NIKPTEGKKET 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 383 TAHHGIIPTQQAADGsKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVT--SAADE 460
Cdd:PRK07219 369 TDHPPIHPVDVPKRE-ELSDDEWKVYELIVRRFLATLADPAEWEYLKVELDVNGEIFKASGSRLVEEGWHEVYpyEKFDE 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 461 DKaddegqaLPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAIIAE 540
Cdd:PRK07219 448 KE-------LPDLEEGEKLKVNKIEIEAKETQPPKRYTQSSLIKEMEKR-----------------GLGTKATRHDIIEK 503
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2527498429 541 LKRKKYLeVKGKKIVATELGLHLL----QVVPDVMKnPVLTALFERILREVEAGnvpldvfiEKQKQLVINELRRL 612
Cdd:PRK07219 504 LYKRGYV-IEGDPPRPTDLGIAVIealeKYAPEIVS-EEMTAQLEADMQAIEDG--------KKKKEDVTEESREM 569
|
|
| PRK05776 |
PRK05776 |
DNA topoisomerase I; Provisional |
3-614 |
7.90e-81 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 235602 [Multi-domain] Cd Length: 670 Bit Score: 268.01 E-value: 7.90e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 3 LFIAEKPSVAKAIVAELGcvtrgDGFITCKDGSVVTWCF-------------GHLLEQAepdaylPDdvprtKKGSKVWR 69
Cdd:PRK05776 4 LVIAEKPKAARKIAEALS-----EKPIRCRIYGVPYWIVkrdgkkivvapaaGHLFGLH------TK-----SKGFPVFD 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 70 FEDLPIYpknwkLLPKNDKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRK 149
Cdd:PRK05776 68 YEWKPLY-----EIDKGSKYTKKYYELLSSLSKYADEFINACDYDIEGSVIGYLIIKYLGDPKKAKRMKFSALTKSDIRR 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 150 GLTNLrdnETFD-GMRLAALGRSRADWLLGMNLSRAYTLA-QQAQGKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYF 227
Cdd:PRK05776 143 AFRNL---ETLDyEMINAGIARHELDWLWGINVSRALMSSvRDASGKRVILSAGRVQSPTLKYVVEREIERNLFVPLPYF 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 228 VIKARL-GGGKPFTAVWEPEESQAgIDEQKRLVDRCiaaalqqrlKAVGQATVVRCSRTPKKIAQPKAFSLADIQLGASN 306
Cdd:PRK05776 220 SVSIIIeKNGYEFTLKYENKKFET-KEEAKEILEEI---------KKTGYLKVTKVEVKIEILEPPPPFNLGDLQVEAAR 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 307 QFGFSAEKTLNLCQSLYEthKA-TSYPRTDCSFLPESqyADAKNVLAAIAKtMP---PLAGLVAKCDCSIQSPTWNDKKI 382
Cdd:PRK05776 290 IYGFSPYKTQSIAEDLYL--DGlISYPRTNSQKLPPT--LNIRNILKGLSR-SPqyrPLVNLLLKETKGVLKPVQGPKDD 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 383 TAHHGIIPTQQAAdgSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATE--RFSAKGRLVTKPGWKVVT--SAA 458
Cdd:PRK05776 365 PAHPAIYPTGEPP--KNLSKDEFKLYDLIVRRFLASFAAPAVLSNTIVTLRVPGFplVFSASGQRIEERGWLKYYpfHKF 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 459 DEDKaddegqaLPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAAII 538
Cdd:PRK05776 443 DEEE-------LPLLKKGERVKIVDVKVRKSYTKPPSRYSKASLLKWMESV-----------------GIGTEATRARII 498
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 539 AELKRKKYLEVKGKKIVATELGLHLLQV----VPDVMKnPVLTALFERILREVEAGNVPLDVFIEKQKQLVINELRRLRK 614
Cdd:PRK05776 499 ETLFKRGYLTSNGKYIEVTPLGFGVAEVlekyFPDIVS-VELTRDFEEKLEMIRTGKATREEVIEEAKETLNKLLEEFKK 577
|
|
| TOP1Ac |
smart00437 |
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, ... |
284-560 |
1.92e-66 |
|
Bacterial DNA topoisomerase I DNA-binding domain; Bacterial DNA topoisomerase I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase alpha subunit
Pssm-ID: 214663 [Multi-domain] Cd Length: 259 Bit Score: 217.88 E-value: 1.92e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 284 RTPKKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQSLYEtHKATSYPRTDCSFLPESQYADAKNvlaAIAKTMPPLAG 363
Cdd:smart00437 2 EKEKKKNPPPPFTTSTLQQEASRKLGFSAKKTMQIAQKLYE-KGLITYPRTDSTRLSEEAVLEARN---YIQKLYGKEYL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 364 LVAKCDCSIQSPTWNDKKITAHHGIIPTQQA---ADGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFS 440
Cdd:smart00437 78 DLAVVLLKPRKPRKGKKAQGAHEAIRPTKPIftpELEKELSEDEFKLYELIWRRFLASQMPDAKYEETKVTIKIGGEKFK 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 441 AKGRLVTKPGWKVVTSAADedkaDDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENihqavndpqskk 520
Cdd:smart00437 158 AKGKTLLFDGWLKVYPEEK----KEEEIELPTLKKGDELKVEEVEVEEKETKPPARYTEASLIKLMEK------------ 221
|
250 260 270 280
....*....|....*....|....*....|....*....|
gi 2527498429 521 flkegDGIGTPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:smart00437 222 -----RGIGRPSTYASIIETLLDRGYVTKKKKKLIPTELG 256
|
|
| PRK07220 |
PRK07220 |
DNA topoisomerase I; Validated |
1-614 |
2.77e-54 |
|
DNA topoisomerase I; Validated
Pssm-ID: 180892 [Multi-domain] Cd Length: 740 Bit Score: 197.29 E-value: 2.77e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGC----VTRGDG-----FITCKDGSVVTWCFGHLLEQAEPDAYlpddvprtkkgskvwrfe 71
Cdd:PRK07220 1 MHLIITEKNIAARRIAQILAPkkpkKTRVSGvdvyrYEDNGDDTVVVGLSGHIVNIDFPKEY------------------ 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 72 dlpiypKNW-KLLPKN--DKGV------KKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVL---EHFRYTGRVQRFWF 139
Cdd:PRK07220 63 ------NNWqKVDARDliDAEIittptqKKIVTALKKLGKEADRVTIATDYDREGELIGVEALniiKKVNPDIKFDRVRY 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 140 SAQDSASIRKGLTNlRDNETFDgmrLAALGRSRA--DWLLGMNLSRAYTLAQQAQGKkELIAVGRVQTPTLALVAKRDVA 217
Cdd:PRK07220 137 SAITKKEIERAFSN-PVEVDFN---LADAGHSRQviDLVWGAALTRYISLAAGRLGK-MFLSVGRVQSPTLALIVDREKE 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 218 IRDFKPVPYFVIKARLG-GGKPFTAVwepeesqagiDEQKRLVDRCIAAALQQRLKAVgqATVVRCSRTPKKIAQPKAFS 296
Cdd:PRK07220 212 REAFVPTPYWEIYATLEnNGETFVAQ----------HSTRRFWEKEEADRVFEKLGKT--AEVTEVEKGTKTDKPPTPFN 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 297 LADIqLGASNQFGFSAEKTLNLCQSLYeTHKATSYPRTDCSFLPESqyADAKNVLAAIAK-TMPPLAG-LVAKcdcSIQS 374
Cdd:PRK07220 280 TTEF-ISAANSIGFSAANAMRIAESLY-TNGYISYPRTDNTVYPES--LDLREQIEIFAEgPFGEYAQkLLEK---GELV 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 375 PTWNDKKITAHHGIIPTQqAADGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVV 454
Cdd:PRK07220 353 PTRGKKETTDHPPIYPAS-LAKKSELKEDEWKVYELVVRRFFATFAGPAEWETMKLRFDIGGEEFRANGSRLTEPGWRWY 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 455 TS-AADEDKaddegqALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPAT 533
Cdd:PRK07220 432 YPyNAPEDR------LLPELSEGEELKVKKKEMLDKETQPPGRYGQGRLIKLMEDL-----------------GLGTKAT 488
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 534 RAAIIAELKRKKYleVKGKKIVATELGLHL---LQVVPDVMKNPVLTALFERILREVEAGNVPLDVFIEKQKQL---VIN 607
Cdd:PRK07220 489 RHEIISKLYSRAY--IHGNPIQPTNTAFAVvdaLEKYAPTITKPDMTSRLEEDMDKIAEGKIKEDAVLEESREMleqVFD 566
|
....*..
gi 2527498429 608 ELRRLRK 614
Cdd:PRK07220 567 ELDKNRE 573
|
|
| PRK14973 |
PRK14973 |
DNA topoisomerase I; Provisional |
1-604 |
8.09e-48 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 184936 [Multi-domain] Cd Length: 936 Bit Score: 180.43 E-value: 8.09e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRgdgFITCKDGSVVTWCF---------GHLLEQaepdaylpDDVPrtkkGSKVWRFE 71
Cdd:PRK14973 1 MHLIIAEKNIAANRIAQILAGKTK---VQVKKDGGVSTYSFddtvvvglrGHVVEV--------DFEP----GYTNWRSE 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 72 DLPiyPK---NWKLLPKNDKgvKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTG---RVQRFWFSAQDSA 145
Cdd:PRK14973 66 EHT--PRsliDADTIKKPTE--KKIVGLIQKLAKKADRVTIATDFDTEGELIGKEAYELVRAVNpkvPIDRARFSAITKE 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 146 SIRKGLTNLRDNEtFDgmrLAALGRSRA--DWLLGMNLSRAYTLAQQaQGKKELIAVGRVQTPTLALVAKRDVAIRDFKP 223
Cdd:PRK14973 142 EIVTAFAEPTDLD-FA---LAAAGEARQiiDLIWGASLTRFISLAAH-RGGDNILSVGRVQSPTLAMIVDREKEIEAFVP 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 224 VPYFVIKArlgggkpftavwEPEESQAGIDEQK---RLVDRCIAAALQQRLKAVGQATVVrcsRTPKKIAQ-PKAFSLAD 299
Cdd:PRK14973 217 EKYWMLSL------------ATEKDGEGIEARHthgRFTDSAAAEAAYDATKEPLVVTEV---KEGHKVDRaPTPFDTTT 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 300 IQLGASnQFGFSAEKTLNLCQSLYeTHKATSYPRTDCSFLPESqyADAKNVLAAIAKTmpPLAGLVAKCDCSIQS-PTWN 378
Cdd:PRK14973 282 FIVAAS-RLGFSAANAMRIAEDLY-MNGYISYPRTDNTIYPKS--LDLNGVLATLAKG--AFSKDVAWVKDNRRPvPTRG 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 379 DKKITAHHGIIPTqQAADGSKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATERFSAKGRLVTKPGWKVVT--S 456
Cdd:PRK14973 356 KKSSTDHPPIHPT-GVATREELGDDRWKLYELVVRRFLATLSPDAEWATMKVNFDAGGEPYTATGGRLLEAGWRTVYpyS 434
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 457 AADEDkaddegqALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIGTPATRAA 536
Cdd:PRK14973 435 EAKEN-------ILPAFALGEKLPILAVNLEEKETQPPARYSQSRLIQRMEEL-----------------GLGTKSTRHE 490
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2527498429 537 IIAELKRKKYLEvkGKKIVATELG---LHLLQVVPDVMKNPVLTALFERILREVEAGNVPLD-VFIEKQKQL 604
Cdd:PRK14973 491 VIGKLVSRKYIE--GNPLRPTLVGravTESLEEHAGTITEPDMTQTLEEHMQQIKERKRTRDdVVTESRKML 560
|
|
| TOPRIM_TopoIA_TopoIII |
cd03362 |
TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type ... |
1-154 |
1.73e-42 |
|
TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA duplex, covalently linking to the 5' phosphoryl end of the DNA break and, allowing the other strand of the duplex to pass through the gap. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173782 [Multi-domain] Cd Length: 151 Bit Score: 150.08 E-value: 1.73e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDG------FITCKDGSVVTWCFGHLLEQAEPDAYlpddvprtKKGSKVWRFEDlP 74
Cdd:cd03362 1 MVLIIAEKPSVAKAIAKILGGGSKKKGkgryyeFYGEGGGYVVTWASGHLLELDFPEEY--------DPWDKVWPLED-P 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 75 IYPKNWKLLPKNDKgvKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGR--VQRFWFSAQDSASIRKGLT 152
Cdd:cd03362 72 LFPAPFKLKVDKGK--KKQFKVLKKLAKRADEIVIATDADREGELIGREILEYAKCVKRkpVKRAWFSSLTPKAIRRAFK 149
|
..
gi 2527498429 153 NL 154
Cdd:cd03362 150 NL 151
|
|
| PRK06599 |
PRK06599 |
DNA topoisomerase I; Validated |
72-611 |
2.10e-39 |
|
DNA topoisomerase I; Validated
Pssm-ID: 235840 [Multi-domain] Cd Length: 675 Bit Score: 153.48 E-value: 2.10e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 72 DLPiyPKNWKLLPKND--------KGVKKQLATIGKLLKKASLVVHAGDPDRE-EQL---LVDEVLEHFRYTGRVQRFWF 139
Cdd:PRK06599 36 DLP--KKKGGVDPDNDfapkyeiiEGKEKVVDALKKAAKKADAVYLATDPDREgEAIawhIAEVLKEAKLKDKNVKRVVF 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 140 SAQDSASIRKGLTNLRDNEtfdgMRL--AALGRSRADWLLGMNLS--------RAytlaqqaqgkkelIAVGRVQTPTLA 209
Cdd:PRK06599 114 NEITKKAVQEAIENPRDID----MDLvdAQQARRALDYLVGFKLSpllwkkvrRG-------------LSAGRVQSVALR 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 210 LVAKRDVAIRDFKPVPYFVIKARL--GGGKPFTAV-WEPEESQAgidEQKRLVDRCIAAALQQRLKAvGQATVVRCSRTP 286
Cdd:PRK06599 177 LICEREDEIEAFIPQEYWTIEADLatSNGEPFTAKlVEVNGKKL---EKFSITNEEQAKALVKALEG-QAYTVDKIEKKE 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 287 KKIAQPKAFSLADIQLGASNQFGFSAEKTLNLCQSLYE-------THKATSYPRTDCSFLPESQYADAKNVL-AAIAKTM 358
Cdd:PRK06599 253 RKRNPPPPFITSTLQQEASRKLGFSAKKTMRIAQKLYEgidlgegTVGLITYMRTDSVRLSNEALDEARKYItKKYGKEY 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 359 PPLAGLVAKcdcsiqsptwNDKKIT--AHHGIIPT------QQAAdgSKFSDDERKIYRLIAERYL-SNFLPA-HEYLAC 428
Cdd:PRK06599 333 LPAKPRVYK----------KKSKNAqeAHEAIRPTsinrtpESLK--PYLTPDQFKLYELIWKRTVaSQMAPAiLDQTSV 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 429 SIELRIATERFSAKGRLVTKPGWKVVTSAADEDKADDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMEN 508
Cdd:PRK06599 401 DIASENGKYVFRATGSVILFPGFLKVYGESKDDEEEDDEKLLPPLKEGEKLKLDELLPEQHFTEPPPRYSEASLVKKLEE 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 509 IhqavndpqskkflkegdGIGTPATRAAIIAELKRKKYLEVKGKKIVATELGlhllQVVpdvmkNPVLTALFERI----- 583
Cdd:PRK06599 481 Y-----------------GIGRPSTYASIISTLQDREYVELEKKRFIPTDLG----RIV-----NDFLVEHFPKYvdydf 534
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 2527498429 584 -------LREVEAGNVP----LDVF-------IEKQKQLVINELRR 611
Cdd:PRK06599 535 tagledeLDEIAEGKKDwkpvLREFwepfisnLEKVEEDVRTEKVT 580
|
|
| PRK08780 |
PRK08780 |
DNA topoisomerase I; Provisional |
79-560 |
3.50e-38 |
|
DNA topoisomerase I; Provisional
Pssm-ID: 181555 [Multi-domain] Cd Length: 780 Bit Score: 150.68 E-value: 3.50e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 79 NWKLLPKNDKGVKkqlaTIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTG-----RVQRFWFSAQDSASIRKGLTN 153
Cdd:PRK08780 53 RYDLIDKNEKHVE----AIAKAAKSADDLYLATDPDREGEAISWHLAEILKERGllkdkPMQRVVFTEITPRAIKEAMAK 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 154 LRD--NETFDgmrlAALGRSRADWLLGMNLSRayTLAQQAQGKkelIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKA 231
Cdd:PRK08780 129 PRDiaSDLVD----AQQARRALDYLVGFNLSP--LLWRKIQRG---LSAGRVQSPALRMIVEREEEIEAFIAREYWSIEA 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 232 RLG-GGKPFTAVWEPEESQAGidEQKRLVDRCIAAALQQRLK--AVGQATVVRCSRTPKKIAQPKAFSLADIQLGASNQF 308
Cdd:PRK08780 200 DCAhPSQPFNAKLIKLDGQKF--EQFTITDGDTAEAARLRIQqaAQGTLHVTDVESKERKRNPAPPFTTSTLQQEASRKL 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 309 GFSAEKTLNLCQSLYE--------THKATSYPRTDCSFLPESQYADAKNVLAAI--AKTMPplaglvakcDCSIQSPTWN 378
Cdd:PRK08780 278 GFTTRRTMQVAQKLYEgvdlgdegSVGLITYMRTDSVNLSQDALAEIRDVIARDygTASLP---------DQPNTYKTKS 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 379 DKKITAHHGIIPTQQ----AADGSKFSDDERKIYRLIAERYL-SNFLPAhEYLACSIELRIATER-FSAKGRLVTKPGWK 452
Cdd:PRK08780 349 KNAQEAHEAVRPTSAlrtpAQVARFLSDDQRRLYELIWKRAVaCQMIPA-TLNTVSVDLAAGSEHvFRATGSTVVVPGFL 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 453 VVTSAADEDKA---DDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENIhqavndpqskkflkegdGIG 529
Cdd:PRK08780 428 AVYEEGKDDKSaedEDEGRKLPPMKEGDNVPLERIRAEQHFTEPPPRYTEASLVKALEEY-----------------GIG 490
|
490 500 510
....*....|....*....|....*....|.
gi 2527498429 530 TPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:PRK08780 491 RPSTYASIISTLQFRKYVEMEGRRFRPTDVG 521
|
|
| TOPRIM_TopoIA |
cd01028 |
TOPRIM_TopoIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the ... |
1-154 |
8.91e-37 |
|
TOPRIM_TopoIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in the type IA family of DNA topoisomerases (TopoIA). This subgroup contains proteins similar to the Type I DNA topoisomerases: E. coli topisomerases I and III, eukaryotic topoisomerase III and, ATP-dependent reverse gyrase found in archaea and thermophilic bacteria. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA. These enzymes cleave one strand of the DNA duplex, covalently link to the 5' phosphoryl end of the DNA break and allow the other strand of the duplex to pass through the gap. Reverse gyrase is also able to insert positive supercoils in the presence of ATP and negative supercoils in the presence of AMPPNP. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173778 [Multi-domain] Cd Length: 142 Bit Score: 133.89 E-value: 8.91e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGCVTRGDGFITCKDGSVVTWCFGHLLEQAEPDAYLPDDvprtkkgsKVWRFEDLPIYPKNw 80
Cdd:cd01028 1 KVLIIAEKPSKAKTIAKILGKGSKKKGFYGEGGGYVVTASVGHLLELPFPEEYVDWD--------KDWPLELFPFEPKY- 71
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2527498429 81 kllpKNDKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFR-YTGRVQRFWFSAQDSASIRKGLTNL 154
Cdd:cd01028 72 ----VVIPDKKKQLKALKKLAKKADEIVLATDPDREGELIAWEILEVLKcDNKPVKRAWFSEITPKAIREAFKNL 142
|
|
| PRK07561 |
PRK07561 |
DNA topoisomerase I subunit omega; Validated |
1-560 |
4.50e-34 |
|
DNA topoisomerase I subunit omega; Validated
Pssm-ID: 236048 [Multi-domain] Cd Length: 859 Bit Score: 138.39 E-value: 4.50e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGcvtrgDGFItckdgsvVTWCFGHLLEqaepdayLP---DDVPRTKKGSKVWRfedLPIYP 77
Cdd:PRK07561 2 KSLVIVESPAKAKTINKYLG-----SDYV-------VKASVGHIRD-------LPtsaSSVPAKEKGALWAR---MGVDP 59
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 78 -KNWKLLPKNDKGVKKQLATIGKLLKKASLVVHAGDPDRE---------EQLLVDEVlehfrytgRVQRFWFSAQDSASI 147
Cdd:PRK07561 60 dHDFEALYEVLPGKEKVVSELKKAAKDADELYLATDPDREgeaiawhllEVLGGDDV--------PVKRVVFNEITKNAI 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 148 RKGLTNLRDnetFDgMRL--AALGRsRA-DWLLGMNLS--------RAytlaqqaqgkkeLIAvGRVQTPTLALVAKRDV 216
Cdd:PRK07561 132 QEAFENPRE---LD-INLvnAQQAR-RFlDRLVGYMVSpllwkkiaRG------------LSA-GRVQSVAVRLIVERER 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 217 AIRDFKPVPYFVIKARL--GGGKPFTAVWepeesqAGIDEQK----RLVDRCIAAALQQRLKAvGQATVVRCSRTPKKiA 290
Cdd:PRK07561 194 EIEAFVPEEYWDIHADLttPRGDAFEARL------THLDGKKfapvDLLNEAQAEAAVAALEG-ARYSVASVEDKPTT-R 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 291 QPKA-FSLADIQLGASNQFGFSAEKTLNLCQSLYETHKATsYPRTDCSFLPESQYADAKNVLAAI--AKTMPPlaglvak 367
Cdd:PRK07561 266 KPSApFTTSTLQQEASRKLGFSVKKTMRIAQRLYEAGYIT-YMRTDSTNLSQEAINAARGYIGDNygKKYLPE------- 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 368 cdcsiqSPTWNDKKIT----AHHGIIPT---QQAADGSKFSDDERKIYRLIAERYL-SNFLPAhEYLACSIELRIATERF 439
Cdd:PRK07561 338 ------KPRQYSSKAKnaqeAHEAIRPSdvfRTPDQLKGLEGDAQRLYELIWKRFVaSQMAPA-RYDSTTVTIAAGDAEL 410
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 440 SAKGRLVTKPGW-KVVTSAADeDKADDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMEnihqavndpqs 518
Cdd:PRK07561 411 RATGRVLRFDGFlKVYVEGRD-DALDDEDRRLPALKVGDALTLEKLDPTQHFTKPPARYSEASLVKELE----------- 478
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 2527498429 519 kkflKEgdGIGTPATRAAIIAELKRKKYLEVKGKKIVATELG 560
Cdd:PRK07561 479 ----KL--GIGRPSTYASIISTIQDRGYVRLENRRFYPEKMG 514
|
|
| PRK06319 |
PRK06319 |
DNA topoisomerase I/SWI domain fusion protein; Validated |
79-581 |
1.42e-32 |
|
DNA topoisomerase I/SWI domain fusion protein; Validated
Pssm-ID: 235778 [Multi-domain] Cd Length: 860 Bit Score: 133.79 E-value: 1.42e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 79 NWKLLPKNDKGVKKqlatIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRYTGRVQRFWFSAQDSASIRKGLTNLRDNE 158
Cdd:PRK06319 53 DYQILPDKEEVINK----ICKLAKKCDVVYLSPDPDREGEAIAWHIANQLPKNTKIQRISFNAITKGAVTEALKHPREID 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 159 tfdgMRL--AALGRSRADWLLGMNLSRayTLAQQAQgKKELIAVGRVQTPTLALVAKRDVAIRDFKPVPYFVIKARLGG- 235
Cdd:PRK06319 129 ----MALvnAQQARRLLDRIVGYKISP--ILSRKLQ-RRSGVSAGRVQSVALKLVVDREKAIEAFVPVEYWNIRVHLKDp 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 236 --GKPFTAV--------WEPEESQAGIDEQKRLVD-RCIAAALQQRLKAvGQATVVRCSRTPKKIAQPKAFSLADIQLGA 304
Cdd:PRK06319 202 ktQKTFWAHlysvdgkkWEKEIPEGKTEDEVLLINsKEKADHIVELLES-ATYTVTRVESKEKRRNAYPPFITSTLQQEA 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 305 SNQFGFSAEKTLNLCQSLYE-----THKAT---SYPRTDCSFL-PESqyadAKNVLAAIAKTMPPlaglvakcDCSIQSP 375
Cdd:PRK06319 281 SRHFRFSSSRTMNIAQTLYEgvdldSEGATgliTYMRTDSVRTdPEA----LKQVRKYIEGTFGK--------EFLPSSP 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 376 T-WNDKKIT--AHHGIIPT--QQAADG--SKFSDDERKIYRLIAERYLSNFLPAHEYLACSIELRIATE-RFSAKGRLVT 447
Cdd:PRK06319 349 NvYTTKKMAqdAHEAIRPTdiTLTPEKlrSKLTEDQYKLYSLIWKRFVASQMIPAIYDTLAIRITTNKGiDLRATGSLLK 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 448 KPGW-KVVTSAADEDKADDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMENihqavndpqskkflkegD 526
Cdd:PRK06319 429 FKGFlAVYEEKRDDEGDEEENIHLPKLHEQDVLTKEELSAEQAFTKPLPRFTEASLVKELEK-----------------S 491
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 2527498429 527 GIGTPATRAAIIAELKRKKYLEVKGKKIVATELGLHLLQVV----PDVMkNPVLTALFE 581
Cdd:PRK06319 492 GIGRPSTYATIMNKIQSREYTLKENQRLRPTELGKIISQFLetnfPRIM-DIGFTALME 549
|
|
| TOP1Bc |
smart00436 |
Bacterial DNA topoisomeraes I ATP-binding domain; Extension of TOPRIM in Bacterial DNA ... |
134-228 |
7.28e-21 |
|
Bacterial DNA topoisomeraes I ATP-binding domain; Extension of TOPRIM in Bacterial DNA topoisomeraes I and III, Eukaryotic DNA topoisomeraes III, reverse gyrase beta subunit
Pssm-ID: 214662 [Multi-domain] Cd Length: 89 Bit Score: 87.25 E-value: 7.28e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 134 VQRFWFSAQDSASIRKGLTNLRDNetFDGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGkkelIAVGRVQTPTLALVAK 213
Cdd:smart00436 1 IKRIEFSEITKKAIREALKNPREI--DENLVNAQLARRILDRLIGFNLSRLLTKKLRNGV----LSAGRVQTPTLGLIVE 74
|
90
....*....|....*
gi 2527498429 214 RDVAIRDFKPVPYFV 228
Cdd:smart00436 75 REREIKNFVPKPYWE 89
|
|
| PTZ00407 |
PTZ00407 |
DNA topoisomerase IA; Provisional |
76-584 |
1.93e-13 |
|
DNA topoisomerase IA; Provisional
Pssm-ID: 173597 [Multi-domain] Cd Length: 805 Bit Score: 73.44 E-value: 1.93e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 76 YPKNWKLLPknDKGVKKQLAT-IGKLLKKASLVVHAGDPDREEQLLVDEVLEHFR---------YTgrvqRFWFSAQDSA 145
Cdd:PTZ00407 98 YTLEWELLP--GRRIQETLERyIEEKADNVTEIILATDPDREGELIAVHALQTIKrlypklkvpFS----RAYMHSITED 171
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 146 SIRKGLTNlRDNETFD-GMRLAALGRSRADWLLGMnLSRAYTLAQQAQGKkeliAVGRVQTPTLALVAKRDVAIRDF--K 222
Cdd:PTZ00407 172 GIRKAMRE-RHVEACDyDLANAAETRHAMDRIFGF-LGSSVVRAANSQMR----SIGRVQTPALILINEREDKIKAFleS 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 223 PVPYFVIKARL----GGGKPFTAVWEPEESQAGIDEQKRlvdrcIAAALQQRLK--AVGQATVVRCSRTPK----KIAQP 292
Cdd:PTZ00407 246 NKSTFEVQAMCqfpsPHGTTFSQVVTITPDRKGGASHWA-----TEAEARRCLEqwKLNNCTGFSVPLEPKpqpsVVPPP 320
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 293 KAFSLADIQLGASNQFGFSAEKTLNLCQSLYETHKATsYPRTDCSFLPESQYADaknVLAAIAKT-----MPPLAGLVAK 367
Cdd:PTZ00407 321 QPFTMATAIAKANRQLKYSSEMVSGCLQDLFQLGHIT-YPRTDSTRIDESALPD---IYAAVKKEfgkefLYRLEDRTVS 396
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 368 CDCSIQSPTWNDKKIT---------------AHHGIIPTQQAADGSKF--SDDERKIYRLIAERYLSNFL-PAH-EYLAC 428
Cdd:PTZ00407 397 AQEGKGSKKTGKKRSTkqkkgadtpvgnvedAHEAIRPTNIDTTGESLslSPPTRAVYDLVRRNTLAVFMiPMKtEKIVA 476
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 429 SIELRIATE---RFSAKGRLVTKPGWK-----------VVTSAADEDK-------------ADDEGQALPELKSGLQLPV 481
Cdd:PTZ00407 477 TVKFTSGSGeklEFELQGKRVVEPGWTrafhkgdkgttGPAEETDEDItaveegapvvpslSQEEFKAIMNLRSQLGSGV 556
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 482 SGIDCE-------EERTKPPAAFTEGTLIRAMENihqavndpqskkflkegDGIGTPATRAAIIAELKRKKYLEVKGK-K 553
Cdd:PTZ00407 557 QKGFFElrspqvrENRPVPPLPHSEGTLIEELKN-----------------NGVGRPSTYPMIVKTLLARGYIAVNPKgR 619
|
570 580 590
....*....|....*....|....*....|....
gi 2527498429 554 IVATELGLHLLQVVPDVMKNPV---LTALFERIL 584
Cdd:PTZ00407 620 CETTPVGRMLVETAKSTFPSIVdigFTAAFEKKL 653
|
|
| Toprim |
pfam01751 |
Toprim domain; This is a conserved region from DNA primase. This corresponds to the Toprim ... |
2-140 |
1.49e-05 |
|
Toprim domain; This is a conserved region from DNA primase. This corresponds to the Toprim domain common to DnaG primases, topoisomerases, OLD family nucleases and RecR proteins. Both DnaG motifs IV and V are present in the alignment, the DxD (V) motif may be involved in Mg2+ binding and mutations to the conserved glutamate (IV) completely abolish DnaG type primase activity. DNA primase EC:2.7.7.6 is a nucleotidyltransferase it synthesizes the oligoribonucleotide primers required for DNA replication on the lagging strand of the replication fork; it can also prime the leading stand and has been implicated in cell division. This family also includes the atypical archaeal A subunit from type II DNA topoisomerases. Type II DNA topoisomerases catalyze the relaxation of DNA supercoiling by causing transient double strand breaks.
Pssm-ID: 396354 [Multi-domain] Cd Length: 93 Bit Score: 43.88 E-value: 1.49e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 2 RLFIAEKPSVAKAIVAELGCVTRgdgfitckdgsVVTWCFGHLLEQAEPDAylpddvprtkkgskvwrfedlpiypknwk 81
Cdd:pfam01751 1 ELIIVEGPSDAIALEKALGGGFQ-----------AVVAVLGHLLSLEKGPK----------------------------- 40
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 82 llpkndkgvKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHFRY-TGRVQRFWFS 140
Cdd:pfam01751 41 ---------KKALKALKELALKAKEVILATDPDREGEAIALKLLELKELlENAGGRVEFS 91
|
|
| COG1754 |
COG1754 |
Uncharacterized C-terminal domain of topoisomerase IA [Function unknown]; |
49-557 |
2.69e-05 |
|
Uncharacterized C-terminal domain of topoisomerase IA [Function unknown];
Pssm-ID: 441360 [Multi-domain] Cd Length: 892 Bit Score: 47.50 E-value: 2.69e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 49 EPDAYLPDDVPRTKKGSKVWRFEDLPIYPKNWKLLPKNDKGVKKQLATIGKLLKKASLVVHAGDPDREEQLLVDEVLEHF 128
Cdd:COG1754 24 PKYAKKKKGVKAVLKKADVAKADDKVVDDDDKKDDVDDDKKKKKKLLKAAAKKKKALALALAADDEDEELLAADDELEEE 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 129 ----RYTGRVQRFWFSAQDSASIRKGLTNLRDNETFDGMRLAALGRSRADWLLGMNLSRAYTLAQQAQGKKELIAVGRVQ 204
Cdd:COG1754 104 kvikLLLLELAVERVARAAAVRAAAAEAARLAALLRRRLVLRRLVRRLLVRLLLLRLVLLLLRLLVARRAARRRRRRRRR 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 205 TPTLALVAKRDVAIRDFKPVPYFVIKARLGGGKPFTAVWEPEESQAGIDEQKRLVDRCIAAALQQRLKAVGQATVVRCSR 284
Cdd:COG1754 184 RARRRELERRRERARAAEAEEAAAAAAAAADAGRAAAARDGKGGGKLAAGGAAAAAAAAAAAAAAAAALAEAAAAAAVRE 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 285 TPKKIAQPKAFSLADIQLGASNQFGFS-AEKTLNLCQSLYETHKATSYPRTDcsflpeSQYADAKNVLAAIAKTMPPLAG 363
Cdd:COG1754 264 PPTPTTTTTAQAATTTTTRAAAARTTRsAQQAARRTRLGGEGTYTTTTTTTT------TTSAAAAAAAAAAAALAAAAAA 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 364 LVAKCDCSIQSPTWNDKKITAHHGIIPTQQ---AADGSKFSDDERKIYRLIAERYLSnflpaheyLACSIELRIATERFS 440
Cdd:COG1754 338 AYAPPPPYYYKKKAAAAAAAAAAAAAAAARrppAARAALLSDDRLLLELLLLRRTAA--------AAAAAAAATATTTAA 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 441 AKGRLVTKPGWKVVTS------------AADEDKADDEGQALPELKSGLQLPVSGIDCEEERTKPPAAFTEGTLIRAMEn 508
Cdd:COG1754 410 AAAAGAGAVGTAAAGAvfffggfllfaeEEDDDEEDDDDDDLPALEEGLPLLKEGVLADQHFTQPPPRYTEAPLVKRME- 488
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 2527498429 509 ihqavndpqSKKFLKEGDGIGTPATRAAIIAELKRKKYLEVKGKKIVAT 557
Cdd:COG1754 489 ---------ELGIGRPSTYASILSTLADRIYVRLDKRRLIPEDKGRLVT 528
|
|
| TOPRIM_TopoIA_TopoI |
cd03363 |
TOPRIM_TopoIA_TopoI: The topoisomerase-primase (TOPRIM) domain found in members of the type IA ... |
1-116 |
1.13e-03 |
|
TOPRIM_TopoIA_TopoI: The topoisomerase-primase (TOPRIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to Escherichia coli DNA topoisomerase I. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA duplex, covalently linking to the 5' phosphoryl end of the DNA break and, allowing the other strand of the duplex to pass through the gap. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases the conserved glutamate is believed to act as a general base in strand joining and, as a general acid in strand cleavage. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.
Pssm-ID: 173783 [Multi-domain] Cd Length: 123 Bit Score: 39.08 E-value: 1.13e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2527498429 1 MRLFIAEKPSVAKAIVAELGcvtrgdgfitckDGSVVTWCFGHLLeqaepdaylpdDVPRTKKGSKVWRFEDLPIYPKnw 80
Cdd:cd03363 1 KKLVIVESPAKAKTIKKYLG------------KEYEVLASVGHIR-----------DLPKKGLGVDGEDDGFEPKYVV-- 55
|
90 100 110
....*....|....*....|....*....|....*.
gi 2527498429 81 kllpknDKGVKKQLATIGKLLKKASLVVHAGDPDRE 116
Cdd:cd03363 56 ------IPGKKKVVKELKKLAKKADEIYLATDPDRE 85
|
|
| |
