NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|2544577432|ref|WP_300361771|]
View 

HlyD family secretion protein [uncultured Pseudoalteromonas sp.]

Protein Classification

HlyD family secretion protein( domain architecture ID 11446287)

HlyD family secretion protein similar to Escherichia coli protein YhiI, Acinetobacter baumannii colistin resistance protein EmrA, and Burkholderia cepacia fusaric acid resistance protein FusE

Gene Ontology:  GO:0022857|GO:0016020|GO:0055085
TCDB:  3.A.1

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
13-331 1.02e-55

Multidrug resistance efflux pump EmrA [Defense mechanisms];


:

Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 184.87  E-value: 1.02e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  13 ISLAGFVVLFLYFVIADLYMPVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYD 92
Cdd:COG1566    14 LLLLALGLALWAAGRNGPDEPVTADGRVEARVVTVAAKVSGRVTEVLVKEGDRVKKGQVLARLDPTDLQAALAQAEAQLA 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  93 DAKLQNQRLDSSVKAIEAQLAAAQAKLHEQTLL------FERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSE 166
Cdd:COG1566    94 AAEAQLARLEAELGAEAEIAAAEAQLAAAQAQLdlaqreLERYQALYKKGAVSQQELDEARAALDAAQAQLEAAQAQLAQ 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 167 AKLA---RGKIGDDNLALRHAQNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIV-ANKADLVADFREK 242
Cdd:COG1566   174 AQAGlreEEELAAAQAQVAQAEAALAQAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVpLDDLWVEAYVPET 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 243 SLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGVSDGQLNANGLLsatessnrwvRDAQRQRIHIQLDEHQmLAKFPSGA 322
Cdd:COG1566   254 DLGRVKPGQPVEVRVDAYPDRVFEGKVTSISPGAGFTSPPKNATG----------NVVQRYPVRIRLDNPD-PEPLRPGM 322


                  ....*....
gi 2544577432 323 RATVQLLPD 331
Cdd:COG1566   323 SATVEIDTE 331
 
Name Accession Description Interval E-value
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
13-331 1.02e-55

Multidrug resistance efflux pump EmrA [Defense mechanisms];


Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 184.87  E-value: 1.02e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  13 ISLAGFVVLFLYFVIADLYMPVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYD 92
Cdd:COG1566    14 LLLLALGLALWAAGRNGPDEPVTADGRVEARVVTVAAKVSGRVTEVLVKEGDRVKKGQVLARLDPTDLQAALAQAEAQLA 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  93 DAKLQNQRLDSSVKAIEAQLAAAQAKLHEQTLL------FERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSE 166
Cdd:COG1566    94 AAEAQLARLEAELGAEAEIAAAEAQLAAAQAQLdlaqreLERYQALYKKGAVSQQELDEARAALDAAQAQLEAAQAQLAQ 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 167 AKLA---RGKIGDDNLALRHAQNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIV-ANKADLVADFREK 242
Cdd:COG1566   174 AQAGlreEEELAAAQAQVAQAEAALAQAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVpLDDLWVEAYVPET 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 243 SLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGVSDGQLNANGLLsatessnrwvRDAQRQRIHIQLDEHQmLAKFPSGA 322
Cdd:COG1566   254 DLGRVKPGQPVEVRVDAYPDRVFEGKVTSISPGAGFTSPPKNATG----------NVVQRYPVRIRLDNPD-PEPLRPGM 322


                  ....*....
gi 2544577432 323 RATVQLLPD 331
Cdd:COG1566   323 SATVEIDTE 331
PRK10476 PRK10476
multidrug transporter subunit MdtN;
15-333 5.36e-38

multidrug transporter subunit MdtN;


Pssm-ID: 182488 [Multi-domain]  Cd Length: 346  Bit Score: 139.01  E-value: 5.36e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  15 LAGFVVLFLYFVI--ADLYmPVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAE--LA 90
Cdd:PRK10476   18 IVALAIVALVFVIwrTDSA-PSTDDAYIDADVVHVASEVGGRIVELAVTENQAVKKGDLLFRIDPRPYELTVAQAQadLA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  91 YDDAKLQN-------QRLDSSVKAIEAQLAAAQAKLHEQTLlfERGESLLKKRSISDQEYEtinanyqssKANVA--AIE 161
Cdd:PRK10476   97 LADAQIMTtqrsvdaERSNAASANEQVERARANAKLATRTL--ERLEPLLAKGYVSAQQVD---------QARTAqrDAE 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 162 AQLSEAKL----ARGKIGDDN--LALRHA-QNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIVANKA- 233
Cdd:PRK10476  166 VSLNQALLqaqaAAAAVGGVDalVAQRAArEAALAIAELHLEDTTVRAPFDGRVVGLKVSVGEFAAPMQPIFTLIDTDHw 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 234 DLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGV-SDGQLNANGLLSATESSNRWVRDAQRQRIHIQLDE- 311
Cdd:PRK10476  246 YAIANFRETDLKNIRVGDCATVYSMIDRGRPFEGKVDSIGWGVlPDDGGNVPRGLPYVPRSINWVRVAQRFPVRIMLDKp 325

                         330       340
                  ....*....|....*....|..
gi 2544577432 312 HQMLAKFpsGARATVQLLPDSS 333
Cdd:PRK10476  326 DPELFRI--GASAVVELRPGAA 345
CusB_dom_1 pfam00529
Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli ...
 33-278 5.23e-30

Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.


Pssm-ID: 425733 [Multi-domain]  Cd Length: 322  Bit Score: 116.75  E-value: 5.23e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  33 PVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQNQRLDSSVKAIEAQL 112
Cdd:pfam00529   9 EAPGRVVVSGNAKAVQPQVSGIVTRVLVKEGDRVKAGDVLFQLDPTDYQAALDSAEAQLAKAQAQVARLQAELDRLQALE 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 113 AAAQAKLHEQTLL------------------------FERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSE-- 166
Cdd:pfam00529  89 SELAISRQDYDGAtaqlraaqaavkaaqaqlaqaqidLARRRVLAPIGGISRESLVTAGALVAQAQANLLATVAQLDQiy 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 167 ----------AKLARGKIGDDNLALRHAQNQLEQAKLNLSYTTVTADTDGKTANLQVTP-GTYANKGQPLMAIVANKADL 235
Cdd:pfam00529 169 vqitqsaaenQAEVRSELSGAQLQIAEAEAELKLAKLDLERTEIRAPVDGTVAFLSVTVdGGTVSAGLRLMFVVPEDNLL 248

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*..
gi 2544577432 236 V-ADFREKSLVHVQIGSTAKVSFDALPGKV---FTGKIVSFEAGVSD 278
Cdd:pfam00529 249 VpGMFVETQLDQVRVGQPVLIPFDAFPQTKtgrFTGVVVGISPDTGP 295
RND_mfp TIGR01730
RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion ...
 45-269 1.98e-26

RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273776 [Multi-domain]  Cd Length: 322  Bit Score: 107.02  E-value: 1.98e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQnqrldssvkaieaqlaaaqakLHEQTL 124
Cdd:TIGR01730  27 ADLAAEVAGKITKISVREGQKVKKGQVLARLDDDDYQLALQAALAQLAAAEAQ---------------------LELAQR 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 125 LFERGESLLKKRSISDQEYETINANYQSSKANVAAIEAqlseaklargkigddnlalrhaqnQLEQAKLNLSYTTVTADT 204
Cdd:TIGR01730  86 SFERAERLVKRNAVSQADLDDAKAAVEAAQADLEAAKA------------------------SLASAQLNLRYTEIRAPF 141

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2544577432 205 DGKTANLQVTPGTYANKGQPLMAIVAN-KADLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKI 269
Cdd:TIGR01730 142 DGTIGRRLVEVGAYVTAGQTLATIVDLdPLEADFSVPERDLPQLRRGQTLTVELDALPGEEFKGKL 207
 
Name Accession Description Interval E-value
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
13-331 1.02e-55

Multidrug resistance efflux pump EmrA [Defense mechanisms];


Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 184.87  E-value: 1.02e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  13 ISLAGFVVLFLYFVIADLYMPVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYD 92
Cdd:COG1566    14 LLLLALGLALWAAGRNGPDEPVTADGRVEARVVTVAAKVSGRVTEVLVKEGDRVKKGQVLARLDPTDLQAALAQAEAQLA 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  93 DAKLQNQRLDSSVKAIEAQLAAAQAKLHEQTLL------FERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSE 166
Cdd:COG1566    94 AAEAQLARLEAELGAEAEIAAAEAQLAAAQAQLdlaqreLERYQALYKKGAVSQQELDEARAALDAAQAQLEAAQAQLAQ 173

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 167 AKLA---RGKIGDDNLALRHAQNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIV-ANKADLVADFREK 242
Cdd:COG1566   174 AQAGlreEEELAAAQAQVAQAEAALAQAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVpLDDLWVEAYVPET 253

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 243 SLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGVSDGQLNANGLLsatessnrwvRDAQRQRIHIQLDEHQmLAKFPSGA 322
Cdd:COG1566   254 DLGRVKPGQPVEVRVDAYPDRVFEGKVTSISPGAGFTSPPKNATG----------NVVQRYPVRIRLDNPD-PEPLRPGM 322


                  ....*....
gi 2544577432 323 RATVQLLPD 331
Cdd:COG1566   323 SATVEIDTE 331
PRK10476 PRK10476
multidrug transporter subunit MdtN;
15-333 5.36e-38

multidrug transporter subunit MdtN;


Pssm-ID: 182488 [Multi-domain]  Cd Length: 346  Bit Score: 139.01  E-value: 5.36e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  15 LAGFVVLFLYFVI--ADLYmPVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAE--LA 90
Cdd:PRK10476   18 IVALAIVALVFVIwrTDSA-PSTDDAYIDADVVHVASEVGGRIVELAVTENQAVKKGDLLFRIDPRPYELTVAQAQadLA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  91 YDDAKLQN-------QRLDSSVKAIEAQLAAAQAKLHEQTLlfERGESLLKKRSISDQEYEtinanyqssKANVA--AIE 161
Cdd:PRK10476   97 LADAQIMTtqrsvdaERSNAASANEQVERARANAKLATRTL--ERLEPLLAKGYVSAQQVD---------QARTAqrDAE 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 162 AQLSEAKL----ARGKIGDDN--LALRHA-QNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIVANKA- 233
Cdd:PRK10476  166 VSLNQALLqaqaAAAAVGGVDalVAQRAArEAALAIAELHLEDTTVRAPFDGRVVGLKVSVGEFAAPMQPIFTLIDTDHw 245

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 234 DLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGV-SDGQLNANGLLSATESSNRWVRDAQRQRIHIQLDE- 311
Cdd:PRK10476  246 YAIANFRETDLKNIRVGDCATVYSMIDRGRPFEGKVDSIGWGVlPDDGGNVPRGLPYVPRSINWVRVAQRFPVRIMLDKp 325

                         330       340
                  ....*....|....*....|..
gi 2544577432 312 HQMLAKFpsGARATVQLLPDSS 333
Cdd:PRK10476  326 DPELFRI--GASAVVELRPGAA 345
AcrA COG0845
Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope ...
 45-279 3.23e-35

Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope biogenesis, Defense mechanisms];


Pssm-ID: 440606 [Multi-domain]  Cd Length: 324  Bit Score: 130.83  E-value: 3.23e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDdaklqnqrldssvkaieaqlaAAQAKLHEQTL 124
Cdd:COG0845    24 VEVRARVSGRVEEVLVDEGDRVKKGQVLARLDPPDLQAALAQAQAQLA---------------------AAQAQLELAKA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 125 LFERGESLLKKRSISDQEYETINANYQSSKANVAAieaqlseaklargkigddnlalrhAQNQLEQAKLNLSYTTVTADT 204
Cdd:COG0845    83 ELERYKALLKKGAVSQQELDQAKAALDQAQAALAA------------------------AQAALEQARANLAYTTIRAPF 138

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2544577432 205 DGKTANLQVTPGTYANKGQPLMAIVA-NKADLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKIVSFEAGVSDG 279
Cdd:COG0845   139 DGVVGERNVEPGQLVSAGTPLFTIADlDPLEVEFDVPESDLARLKVGQPVTVTLDAGPGKTFEGKVTFIDPAVDPA 214
CusB_dom_1 pfam00529
Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli ...
 33-278 5.23e-30

Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.


Pssm-ID: 425733 [Multi-domain]  Cd Length: 322  Bit Score: 116.75  E-value: 5.23e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  33 PVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQNQRLDSSVKAIEAQL 112
Cdd:pfam00529   9 EAPGRVVVSGNAKAVQPQVSGIVTRVLVKEGDRVKAGDVLFQLDPTDYQAALDSAEAQLAKAQAQVARLQAELDRLQALE 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 113 AAAQAKLHEQTLL------------------------FERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSE-- 166
Cdd:pfam00529  89 SELAISRQDYDGAtaqlraaqaavkaaqaqlaqaqidLARRRVLAPIGGISRESLVTAGALVAQAQANLLATVAQLDQiy 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 167 ----------AKLARGKIGDDNLALRHAQNQLEQAKLNLSYTTVTADTDGKTANLQVTP-GTYANKGQPLMAIVANKADL 235
Cdd:pfam00529 169 vqitqsaaenQAEVRSELSGAQLQIAEAEAELKLAKLDLERTEIRAPVDGTVAFLSVTVdGGTVSAGLRLMFVVPEDNLL 248

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*..
gi 2544577432 236 V-ADFREKSLVHVQIGSTAKVSFDALPGKV---FTGKIVSFEAGVSD 278
Cdd:pfam00529 249 VpGMFVETQLDQVRVGQPVLIPFDAFPQTKtgrFTGVVVGISPDTGP 295
RND_mfp TIGR01730
RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion ...
 45-269 1.98e-26

RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273776 [Multi-domain]  Cd Length: 322  Bit Score: 107.02  E-value: 1.98e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQnqrldssvkaieaqlaaaqakLHEQTL 124
Cdd:TIGR01730  27 ADLAAEVAGKITKISVREGQKVKKGQVLARLDDDDYQLALQAALAQLAAAEAQ---------------------LELAQR 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 125 LFERGESLLKKRSISDQEYETINANYQSSKANVAAIEAqlseaklargkigddnlalrhaqnQLEQAKLNLSYTTVTADT 204
Cdd:TIGR01730  86 SFERAERLVKRNAVSQADLDDAKAAVEAAQADLEAAKA------------------------SLASAQLNLRYTEIRAPF 141

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2544577432 205 DGKTANLQVTPGTYANKGQPLMAIVAN-KADLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKI 269
Cdd:TIGR01730 142 DGTIGRRLVEVGAYVTAGQTLATIVDLdPLEADFSVPERDLPQLRRGQTLTVELDALPGEEFKGKL 207
PRK10559 PRK10559
p-hydroxybenzoic acid efflux pump subunit AaeA;
6-326 8.54e-24

p-hydroxybenzoic acid efflux pump subunit AaeA;


Pssm-ID: 182548 [Multi-domain]  Cd Length: 310  Bit Score: 99.43  E-value: 8.54e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432   6 KFARY---VKISLAGFVVLFLYFViadLYM--PVTPQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPF 80
Cdd:PRK10559    7 KISRTaitLVLVILAFIAIFRAWV---FYTesPWTRDARFSADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRY 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  81 ELAVQQAE--LAYDDAKLQNQRLDSSVKAIeaqlaaaqaklheqtllfergeslLKKRSISDQEYETINANYQSSkanva 158
Cdd:PRK10559   84 QKALAEAEadVAYYQVLAQEKRREAGRRNR------------------------LGVQAMSREEIDQANNVLQTV----- 134

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 159 aieaqlsEAKLARgkigddnlalrhAQNQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYANKGQPLMAIVA-NKADLVA 237
Cdd:PRK10559  135 -------LHQLAK------------AQATRDLAKLDLERTVIRAPADGWVTNLNVYTGEFITRGSTAVALVKqNSFYVLA 195

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 238 DFREKSLVHVQIGSTAKVSfdalP---GKVFTGKIVSFEAGVSDGQLNANGLLSATESSN-RWVRDAQRQRIHIQLDeHQ 313
Cdd:PRK10559  196 YMEETKLEGVRPGYRAEIT----PlgsNKVLKGTVDSVAAGVTNSSSTRDSKGMATIDSNlEWVRLAQRVPVRIRLD-NQ 270

                         330
                  ....*....|...
gi 2544577432 314 MLAKFPSGARATV 326
Cdd:PRK10559  271 QGNLYPAGTTATV 283
PRK15136 PRK15136
multidrug efflux MFS transporter periplasmic adaptor subunit EmrA;
45-319 9.42e-18

multidrug efflux MFS transporter periplasmic adaptor subunit EmrA;


Pssm-ID: 185090 [Multi-domain]  Cd Length: 390  Bit Score: 83.59  E-value: 9.42e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQNQRLDSSVKAIEAQLAAAQAKLHEQTL 124
Cdd:PRK15136   62 VQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDAEQAFEKAKTALANSVRQTHQLMINSKQYQANIELQKTALAQAQS 141

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 125 LFERGESLLKKRSISDQEYetinanyQSSKANVAAIEAQLSEAK----LARGKIGDDNL----ALRHAQNQLEQAKLNLS 196
Cdd:PRK15136  142 DLNRRVPLGNANLIGREEL-------QHARDAVASAQAQLDVAIqqynANQAMILNTPLedqpAVQQAATEVRNAWLALQ 214

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 197 YTTVTADTDGKTANLQVTPGTYANKGQPLMAIV-ANKADLVADFREKSLVHVQIGSTAKVSFDAL-PGKVFTGKIVsfea 274
Cdd:PRK15136  215 RTKIVSPMTGYVSRRSVQVGAQISPTTPLMAVVpATNLWVDANFKETQLANMRIGQPATITSDIYgDDVVYTGKVV---- 290

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*
gi 2544577432 275 GVSDGQLNANGLLSATESSNRWVRDAQRQRIHIQLDEHQmLAKFP 319
Cdd:PRK15136  291 GLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDAKQ-LAQHP 334
HlyD_D23 pfam16576
Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and ...
 141-269 6.07e-11

Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.


Pssm-ID: 435440 [Multi-domain]  Cd Length: 214  Bit Score: 61.37  E-value: 6.07e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 141 QEYetINAnYQSSKANVAAIEAQLSEAKLArgkigddNLALRHAQ-NQLEQAKLNLSYTTVTADTDGKTANLQVTPGTYA 219
Cdd:pfam16576  61 QEY--LLA-LRSGDALSKSELLRAARQRLR-------LLGMPEAQiAELERTGKVQPTVTVYAPISGVVTELNVREGMYV 130

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2544577432 220 NKGQPLMAIvankADL-----VADFREKSLVHVQIGSTAKVSFDALPGKVFTGKI 269
Cdd:pfam16576 131 QPGDTLFTI----ADLstvwvEADVPEQDLALVKVGQPAEVTLPALPGKTFEGKV 181
PRK03598 PRK03598
putative efflux pump membrane fusion protein; Provisional
 45-269 1.18e-10

putative efflux pump membrane fusion protein; Provisional


Pssm-ID: 235136 [Multi-domain]  Cd Length: 331  Bit Score: 61.90  E-value: 1.18e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQNQRL-----DSSVKAIEAQLAAAQAKL 119
Cdd:PRK03598   44 VNLGFRVGGRLASLAVDEGDAVKAGQVLGELDAAPYENALMQAKANVSVAQAQLDLMlagyrDEEIAQARAAVKQAQAAY 123

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 120 HEQTLLFERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSEakLARG----KIGDDNLALRHAQNQLEQAKLNL 195
Cdd:PRK03598  124 DYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQATLKSAQDKLSQ--YREGnrpqDIAQAKASLAQAQAALAQAELNL 201

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2544577432 196 SYTTVTADTDGKTANLQVTPGTYANKGQPLMAIVANKADLV-ADFREKSLVHVQIGSTAKVSFDALPGKVFTGKI 269
Cdd:PRK03598  202 QDTELIAPSDGTILTRAVEPGTMLNAGSTVFTLSLTRPVWVrAYVDERNLGQAQPGRKVLLYTDGRPDKPYHGQI 276
Biotin_lipoyl_2 pfam13533
Biotin-lipoyl like;
43-88 3.28e-09

Biotin-lipoyl like;


Pssm-ID: 433286  Cd Length: 50  Bit Score: 52.06  E-value: 3.28e-09
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 2544577432  43 PVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAE 88
Cdd:pfam13533   1 PVVKIASPVSGKVVAVNVKEGQQVKKGDVLATLDSPELQLQLQQAE 46
PRK09578 PRK09578
MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;
46-224 1.69e-08

MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 169982 [Multi-domain]  Cd Length: 385  Bit Score: 55.57  E-value: 1.69e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  46 QVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYddAKLQNQRLDSSVKAieaqlaaaqaklheqtll 125
Cdd:PRK09578   65 EVRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAAGAL--AKAEAAHLAALDKR------------------ 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 126 fERGESLLKKRSISDQEYETINANYQSSKANVAAieaqlseaklargkigddnlalrhAQNQLEQAKLNLSYTTVTADTD 205
Cdd:PRK09578  125 -RRYDDLVRDRAVSERDYTEAVADERQAKAAVAS------------------------AKAELARAQLQLDYATVTAPID 179

                         170
                  ....*....|....*....
gi 2544577432 206 GKTANLQVTPGTYANKGQP 224
Cdd:PRK09578  180 GRARRALVTEGALVGQDQA 198
PRK11556 PRK11556
MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;
45-222 1.20e-06

MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 183194 [Multi-domain]  Cd Length: 415  Bit Score: 49.79  E-value: 1.20e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAE--LAYDDAKLQNQRLDssvkaieaqlaaaqaklheq 122
Cdd:PRK11556   88 VTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPRPFKVALAQAQgqLAKDQATLANARRD-------------------- 147

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 123 tllFERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQLSeaklargkigddnlalrhaqnqleQAKLNLSYTTVTA 202
Cdd:PRK11556  148 ---LARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEASVA------------------------SAQLQLDYSRITA 200

                         170       180
                  ....*....|....*....|
gi 2544577432 203 DTDGKTANLQVTPGTYANKG 222
Cdd:PRK11556  201 PISGRVGLKQVDVGNQISSG 220
PRK09859 PRK09859
multidrug transporter subunit MdtE;
36-282 2.42e-06

multidrug transporter subunit MdtE;


Pssm-ID: 137559 [Multi-domain]  Cd Length: 385  Bit Score: 48.94  E-value: 2.42e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  36 PQARVYHPVVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAE--LAYDDAKLQNQRLDssvkaieaqla 113
Cdd:PRK09859   53 PGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKgsLAKALSTASNARIT----------- 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 114 aaqaklheqtllFERGESLLKKRSISDQEYETINANYQSSKANVAAieaqlseaklargkigddnlalrhAQNQLEQAKL 193
Cdd:PRK09859  122 ------------FNRQASLLKTNYVSRQDYDTARTQLNEAEANVTV------------------------AKAAVEQATI 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 194 NLSYTTVTADTDGKTANLQVTPGTYankgqplmaIVANKADLVADFREKSLVHVQIGSTAKvSFDALPGKVFTGKIVSFE 273
Cdd:PRK09859  166 NLQYANVTSPITGVSGKSSVTVGAL---------VTANQADSLVTVQRLDPIYVDLTQSVQ-DFLRMKEEVASGQIKQVQ 235


                  ....*....
gi 2544577432 274 aGVSDGQLN 282
Cdd:PRK09859  236 -GSTPVQLN 243
PRK11578 PRK11578
macrolide transporter subunit MacA; Provisional
 45-269 8.06e-06

macrolide transporter subunit MacA; Provisional


Pssm-ID: 183211 [Multi-domain]  Cd Length: 370  Bit Score: 47.08  E-value: 8.06e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  45 VQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDEPFELAVQQAELAYDDAKLQNQRldssvkaieaqlaaAQAKLHEQTL 124
Cdd:PRK11578   62 VDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQ--------------AEAELKLARV 127

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 125 LFERGESLLKKRSISDQEYETINANYQSSKANVAAIEAQlseaklargkigddnlaLRHAQNQLEQAKLNLSYTTVTADT 204
Cdd:PRK11578  128 TLSRQQRLAKTQAVSQQDLDTAATELAVKQAQIGTIDAQ-----------------IKRNQASLDTAKTNLDYTRIVAPM 190

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2544577432 205 DGKTANLQVTPG--TYANKGQPLMAIVANKADLV--ADFREKSLVHVQIGStaKVSFDAL--PGKVFTGKI 269
Cdd:PRK11578  191 AGEVTQITTLQGqtVIAAQQAPNILTLADMSTMLvkAQVSEADVIHLKPGQ--KAWFTVLgdPLTRYEGVL 259
PRK15030 PRK15030
multidrug efflux RND transporter periplasmic adaptor subunit AcrA;
44-223 1.69e-05

multidrug efflux RND transporter periplasmic adaptor subunit AcrA;


Pssm-ID: 184990 [Multi-domain]  Cd Length: 397  Bit Score: 46.25  E-value: 1.69e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432  44 VVQVTPQVSGRVTDVLVTNNQAVEKQQALFKINDepfelAVQQAelAYDDAKLQNQRLDSSVKAIEaqlaaaqaklheqt 123
Cdd:PRK15030   65 IAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDP-----ATYQA--TYDSAKGDLAKAQAAANIAQ-------------- 123

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2544577432 124 LLFERGESLLKKRSISDQEYETINANYQSSKANVAAieaqlseaklargkigddnlalrhAQNQLEQAKLNLSYTTVTAD 203
Cdd:PRK15030  124 LTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTA------------------------AKAAVETARINLAYTKVTSP 179

                         170       180
                  ....*....|....*....|
gi 2544577432 204 TDGKTANLQVTPGTYANKGQ 223
Cdd:PRK15030  180 ISGRIGKSNVTEGALVQNGQ 199
AccB COG0511
Biotin carboxyl carrier protein [Lipid transport and metabolism]; Biotin carboxyl carrier ...
 51-75 5.02e-03

Biotin carboxyl carrier protein [Lipid transport and metabolism]; Biotin carboxyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis


Pssm-ID: 440277 [Multi-domain]  Cd Length: 136  Bit Score: 36.80  E-value: 5.02e-03
                          10        20
                  ....*....|....*....|....*
gi 2544577432  51 VSGRVTDVLVTNNQAVEKQQALFKI 75
Cdd:COG0511   111 VSGTVVEILVENGQPVEYGQPLFVI 135
HlyD_3 pfam13437
HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator ...
 199-271 8.55e-03

HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator HlyD proteins.


Pssm-ID: 433206 [Multi-domain]  Cd Length: 104  Bit Score: 35.42  E-value: 8.55e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2544577432 199 TVTADTDGKTANLQVTPGTYANKGQPLMAIVA-NKADLVADFREKSLVHVQIGSTAKVSFDALPGKVFTGKIVS 271
Cdd:pfam13437   1 TIRAPVDGVVAELNVEEGQVVQAGDPLATIVPpDRLLVEAFVPAADLGSLKKGQKVTLKLDPGSDYTLEGKVVR 74
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH