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Conserved domains on  [gi|2569278746|ref|WP_306711786|]
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nucleotidyltransferase domain-containing protein [Haloarcula sp. H-GB4]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
105-206 2.64e-08

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


:

Pssm-ID: 441314  Cd Length: 95  Bit Score: 50.03  E-value: 2.64e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746 105 PIRTFVDRAQAALTDADDVdelLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRitdvvgDLQSDRFDGERFAFEP 184
Cdd:COG1708     4 LLRELLEEIVEALRRGPEV---AAVYLFGSYARGDARPDSDIDLLVVVDDPPLPDERL------ELLADLLRELGLPVDL 74

                          90       100
                  ....*....|....*....|..
gi 2569278746 185 YVESAESaYRAGSKLREIFAEG 206
Cdd:COG1708    75 VVLTPAE-LELRLFLREILREG 95
COG2512 COG2512
Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain ...
 6-85 8.84e-07

Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain [General function prediction only];


:

Pssm-ID: 442002 [Multi-domain]  Cd Length: 80  Bit Score: 45.29  E-value: 8.84e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746   6 ITVSIDAYPDPDTDvfrisaADDILRLLVDaHDTEFTIPELVDATGVTRSTVWRAVSLLDSIGAIQIRETPQRNYITINP 85
Cdd:COG2512     3 VVAALLLYPELLED------ERRVLELLRE-NGGRMTQSEIVKETGWSKSKVSRLLSRLEERGLIEKERVGRENVVELPE 75
 
Name Accession Description Interval E-value
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
105-206 2.64e-08

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 50.03  E-value: 2.64e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746 105 PIRTFVDRAQAALTDADDVdelLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRitdvvgDLQSDRFDGERFAFEP 184
Cdd:COG1708     4 LLRELLEEIVEALRRGPEV---AAVYLFGSYARGDARPDSDIDLLVVVDDPPLPDERL------ELLADLLRELGLPVDL 74

                          90       100
                  ....*....|....*....|..
gi 2569278746 185 YVESAESaYRAGSKLREIFAEG 206
Cdd:COG1708    75 VVLTPAE-LELRLFLREILREG 95
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 129-163 8.51e-07

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 45.87  E-value: 8.51e-07
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2569278746 129 IVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRI 163
Cdd:pfam01909  17 VVLFGSYARGTALPGSDIDLLVVFPEPVEEERLLK 51
COG2512 COG2512
Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain ...
 6-85 8.84e-07

Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain [General function prediction only];


Pssm-ID: 442002 [Multi-domain]  Cd Length: 80  Bit Score: 45.29  E-value: 8.84e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746   6 ITVSIDAYPDPDTDvfrisaADDILRLLVDaHDTEFTIPELVDATGVTRSTVWRAVSLLDSIGAIQIRETPQRNYITINP 85
Cdd:COG2512     3 VVAALLLYPELLED------ERRVLELLRE-NGGRMTQSEIVKETGWSKSKVSRLLSRLEERGLIEKERVGRENVVELPE 75
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 124-163 5.01e-06

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 43.56  E-value: 5.01e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 2569278746 124 DELLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRI 163
Cdd:cd05403    16 GGVEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELAR 55
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 28-80 5.48e-05

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 39.72  E-value: 5.48e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2569278746  28 DILRLLVDAHDTeFTIPELVDATGVTRSTVWRAVSLLDSIGaIQIRETPQRNY 80
Cdd:pfam08279   2 QILQLLLEARGP-ISGQELAEKLGVSRRTIRRDIKILEELG-VPIEAEPGRGY 52
 
Name Accession Description Interval E-value
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
105-206 2.64e-08

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 50.03  E-value: 2.64e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746 105 PIRTFVDRAQAALTDADDVdelLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRitdvvgDLQSDRFDGERFAFEP 184
Cdd:COG1708     4 LLRELLEEIVEALRRGPEV---AAVYLFGSYARGDARPDSDIDLLVVVDDPPLPDERL------ELLADLLRELGLPVDL 74

                          90       100
                  ....*....|....*....|..
gi 2569278746 185 YVESAESaYRAGSKLREIFAEG 206
Cdd:COG1708    75 VVLTPAE-LELRLFLREILREG 95
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 129-163 8.51e-07

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 45.87  E-value: 8.51e-07
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2569278746 129 IVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRI 163
Cdd:pfam01909  17 VVLFGSYARGTALPGSDIDLLVVFPEPVEEERLLK 51
COG2512 COG2512
Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain ...
 6-85 8.84e-07

Predicted transcriptional regulator, contains CW (cell wall-binding) repeats and an HTH domain [General function prediction only];


Pssm-ID: 442002 [Multi-domain]  Cd Length: 80  Bit Score: 45.29  E-value: 8.84e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746   6 ITVSIDAYPDPDTDvfrisaADDILRLLVDaHDTEFTIPELVDATGVTRSTVWRAVSLLDSIGAIQIRETPQRNYITINP 85
Cdd:COG2512     3 VVAALLLYPELLED------ERRVLELLRE-NGGRMTQSEIVKETGWSKSKVSRLLSRLEERGLIEKERVGRENVVELPE 75
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 124-163 5.01e-06

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 43.56  E-value: 5.01e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 2569278746 124 DELLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRI 163
Cdd:cd05403    16 GGVEKVYLFGSYARGDARPDSDIDLLVIFDDPLDPLELAR 55
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
129-160 1.16e-05

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


Pssm-ID: 441275  Cd Length: 96  Bit Score: 42.59  E-value: 1.16e-05
                          10        20        30
                  ....*....|....*....|....*....|..
gi 2569278746 129 IVVFGSVARGEADRQSDIDCFVVVDGDRTTAR 160
Cdd:COG1669    26 LGLFGSVARGEAREDSDIDLLVEFDEPTSLFD 57
BirA COG1654
Biotin operon repressor [Transcription];
22-162 1.43e-05

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 44.98  E-value: 1.43e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746  22 RISAADDILRLLvdAHDTEFTIPELVDATGVTRSTVWRAVSLLDSIGaIQIRETPQRNYITINPNRLQKDDPILAIPQSE 101
Cdd:COG1654     2 MSSTRLKLLRLL--ADGEFHSGEELAEELGVSRAAVWKHIKALRELG-YEIESVPGKGYRLAEPPDLLDPEEIRAGLSTK 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2569278746 102 FHAPIRTFVDraqaalTDADDVDELLgivvfgsvargEADRQSDIDCFVVVDGDRTTARRR 162
Cdd:COG1654    79 RLGREILYVI------SSTSTNLLAL-----------ELAAQGGDAGTVVAAEQQRGGRGR 122
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 28-80 5.48e-05

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 39.72  E-value: 5.48e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2569278746  28 DILRLLVDAHDTeFTIPELVDATGVTRSTVWRAVSLLDSIGaIQIRETPQRNY 80
Cdd:pfam08279   2 QILQLLLEARGP-ISGQELAEKLGVSRRTIRRDIKILEELG-VPIEAEPGRGY 52
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 124-214 2.06e-03

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


Pssm-ID: 465859  Cd Length: 93  Bit Score: 36.35  E-value: 2.06e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2569278746 124 DELLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRRitDVVGDLqSDRFDGERFAFepyVEsaesaYRAGSKL--RE 201
Cdd:pfam18765  11 PEIEAAYLFGSRAKGDAREDSDIDLAVLYDEKLDFEELL--ELASEL-EDILLLRKVDL---VD-----LNKAPPVlaHQ 79

                          90
                  ....*....|...
gi 2569278746 202 IFAEGITVYGSDQ 214
Cdd:pfam18765  80 ILKEGKLLYSRDE 92
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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