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Conserved domains on  [gi|2576161605|ref|WP_309474058|]
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MlaD family protein [Mycolicibacterium chitae]

Protein Classification

MlaD family protein( domain architecture ID 11444651)

MlaD family protein similar to MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane, and to mce (mammalian cell entry) proteins from Mycobacterium tuberculosis, virulence proteins required for invasion of non-phagocytic cells

Gene Ontology:  GO:0015914
PubMed:  28819315|34196044

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 22-208 9.05e-26

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 100.17  E-value: 9.05e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  22 SSRRLLLSIVgqLTLVAVGVAYLAFGALRVNPFDTQMAVQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDG--VLAT 99
Cdd:COG1463     4 RSRELKVGLF--VLVGLALLVGLALWLGGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyrVRVT 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605 100 ALIDGDVRIPlADTAVRVSALSPAGEQYLDFSPRGSDGPFIEDGTIIgrdRTETPVPLWRLLTNVDGVLAQADPAQISAV 179
Cdd:COG1463    82 LRIDPDVPIP-ADSVATIRSSGLLGEKYVELSPGGDSGPPLADGDVI---PLESPVDLEDLLSQLSPLLEALDPGALLDN 157

                         170       180       190
                  ....*....|....*....|....*....|
gi 2576161605 180 IDEL-GVSEQGPEKLRDLFNGAQMLLSTLD 208
Cdd:COG1463   158 LNTLtDTLADAAAQLDALLDNLNTLSDTLA 187
 
Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 22-208 9.05e-26

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 100.17  E-value: 9.05e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  22 SSRRLLLSIVgqLTLVAVGVAYLAFGALRVNPFDTQMAVQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDG--VLAT 99
Cdd:COG1463     4 RSRELKVGLF--VLVGLALLVGLALWLGGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyrVRVT 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605 100 ALIDGDVRIPlADTAVRVSALSPAGEQYLDFSPRGSDGPFIEDGTIIgrdRTETPVPLWRLLTNVDGVLAQADPAQISAV 179
Cdd:COG1463    82 LRIDPDVPIP-ADSVATIRSSGLLGEKYVELSPGGDSGPPLADGDVI---PLESPVDLEDLLSQLSPLLEALDPGALLDN 157

                         170       180       190
                  ....*....|....*....|....*....|
gi 2576161605 180 IDEL-GVSEQGPEKLRDLFNGAQMLLSTLD 208
Cdd:COG1463   158 LNTLtDTLADAAAQLDALLDNLNTLSDTLA 187
Mtu_fam_mce TIGR00996
virulence factor Mce family protein; Members of this paralogous family are found as six tandem ...
 27-208 2.41e-24

virulence factor Mce family protein; Members of this paralogous family are found as six tandem homologous proteins in the same orientation per cassette, in four separate cassettes in Mycobacterium tuberculosis. The six members of each cassette represent six subfamilies. One subfamily includes the protein mce (mycobacterial cell entry), a virulence protein required for invasion of non-phagocytic cells. [Cellular processes, Pathogenesis]


Pssm-ID: 273384 [Multi-domain]  Cd Length: 291  Bit Score: 97.36  E-value: 2.41e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  27 LLSIVGQLTLVAVGVAYLAFGALRVNPFD-TQMAVQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDGVLATALIDGD 105
Cdd:TIGR00996   1 RLGTVAVVVLALVVVLAVVFLRLPFTGFGpGTTTVVAYFTDAGGLYPGDKVRVAGVPVGKVTAIEPRGARARVTFSLDRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605 106 VRIPlADTAVRVSALSPAGEQYLDFSPRGSD-GPFIEDGTIIGRDRTETPVPLWRLLTNVDGVLAQADPAQISAVIDEL- 183
Cdd:TIGR00996  81 VTLP-ANATAAIRTTTLLGSRYIELTPPGGPpGGRLSDGGTIPLSRTTVPLELDDLLGSLTRLLNGLDPEKLNAILNALa 159

                         170       180
                  ....*....|....*....|....*.
gi 2576161605 184 -GVSEQGPEkLRDLFNGAQMLLSTLD 208
Cdd:TIGR00996 160 qALAGQGPQ-LRNLLDGLAQLTAALN 184
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 60-133 3.80e-10

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 54.51  E-value: 3.80e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2576161605  60 VQVQLDESGGLLASQDVTLRGIPVGRVQSVEV--TGDGVLATALIDGDVRIPL-ADTAVRVSALSPAGEQYLDFSPR 133
Cdd:pfam02470   5 ITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLdpDGKGVRVTLRIDPDYDVLLpKDSRAGIRSLGLLGGKYIALDPG 81
 
Name Accession Description Interval E-value
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 22-208 9.05e-26

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 100.17  E-value: 9.05e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  22 SSRRLLLSIVgqLTLVAVGVAYLAFGALRVNPFDTQMAVQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDG--VLAT 99
Cdd:COG1463     4 RSRELKVGLF--VLVGLALLVGLALWLGGLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGyrVRVT 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605 100 ALIDGDVRIPlADTAVRVSALSPAGEQYLDFSPRGSDGPFIEDGTIIgrdRTETPVPLWRLLTNVDGVLAQADPAQISAV 179
Cdd:COG1463    82 LRIDPDVPIP-ADSVATIRSSGLLGEKYVELSPGGDSGPPLADGDVI---PLESPVDLEDLLSQLSPLLEALDPGALLDN 157

                         170       180       190
                  ....*....|....*....|....*....|
gi 2576161605 180 IDEL-GVSEQGPEKLRDLFNGAQMLLSTLD 208
Cdd:COG1463   158 LNTLtDTLADAAAQLDALLDNLNTLSDTLA 187
Mtu_fam_mce TIGR00996
virulence factor Mce family protein; Members of this paralogous family are found as six tandem ...
 27-208 2.41e-24

virulence factor Mce family protein; Members of this paralogous family are found as six tandem homologous proteins in the same orientation per cassette, in four separate cassettes in Mycobacterium tuberculosis. The six members of each cassette represent six subfamilies. One subfamily includes the protein mce (mycobacterial cell entry), a virulence protein required for invasion of non-phagocytic cells. [Cellular processes, Pathogenesis]


Pssm-ID: 273384 [Multi-domain]  Cd Length: 291  Bit Score: 97.36  E-value: 2.41e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  27 LLSIVGQLTLVAVGVAYLAFGALRVNPFD-TQMAVQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDGVLATALIDGD 105
Cdd:TIGR00996   1 RLGTVAVVVLALVVVLAVVFLRLPFTGFGpGTTTVVAYFTDAGGLYPGDKVRVAGVPVGKVTAIEPRGARARVTFSLDRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605 106 VRIPlADTAVRVSALSPAGEQYLDFSPRGSD-GPFIEDGTIIGRDRTETPVPLWRLLTNVDGVLAQADPAQISAVIDEL- 183
Cdd:TIGR00996  81 VTLP-ANATAAIRTTTLLGSRYIELTPPGGPpGGRLSDGGTIPLSRTTVPLELDDLLGSLTRLLNGLDPEKLNAILNALa 159

                         170       180
                  ....*....|....*....|....*.
gi 2576161605 184 -GVSEQGPEkLRDLFNGAQMLLSTLD 208
Cdd:TIGR00996 160 qALAGQGPQ-LRNLLDGLAQLTAALN 184
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 60-133 3.80e-10

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 54.51  E-value: 3.80e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2576161605  60 VQVQLDESGGLLASQDVTLRGIPVGRVQSVEV--TGDGVLATALIDGDVRIPL-ADTAVRVSALSPAGEQYLDFSPR 133
Cdd:pfam02470   5 ITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLdpDGKGVRVTLRIDPDYDVLLpKDSRAGIRSLGLLGGKYIALDPG 81
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 37-142 7.62e-06

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 44.00  E-value: 7.62e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2576161605  37 VAVGVAYLAFGALRVNPFDTQMA-----VQVQLDESGGLLASQDVTLRGIPVGRVQSVEVTGDGVLA--TALIDGDVRIP 109
Cdd:TIGR04430  13 VLAGAAALLFLALKVGNLSSFSSgdtytVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAvvTLSIDKDYKLP 92

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 2576161605 110 lADTAVRVSALSPAGEQYLDFSPRGS-----DGPFIED 142
Cdd:TIGR04430  93 -DDSSASILTSGLLGEQYIGLSPGGDedylkDGDEIED 129
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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