|
Name |
Accession |
Description |
Interval |
E-value |
| NRPS_term_dom |
TIGR02353 |
non-ribosomal peptide synthetase terminal domain of unknown function; This domain is found ... |
601-1302 |
0e+00 |
|
non-ribosomal peptide synthetase terminal domain of unknown function; This domain is found exclusively in non-ribosomal peptide synthetases and always as the final domain in the polypeptide. This domain is roughly 700 amino acids in size and is found in polypeptides roughly twice that size.
Pssm-ID: 274093 [Multi-domain] Cd Length: 695 Bit Score: 676.47 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 601 LAQALTLPALIALHIMNWLAPFFTYHYYTGEPEDgVAFAVLASVLVFLASHLVSLGIAMSGSRLLLSGLKAGRYPLWGGN 680
Cdd:TIGR02353 1 VCQTLQLIPIVTLSGLQWLAPLLGYNWLYEALDD-VSWLYLRAVALVFAVPVGRLGFAIAAKWLLVGRWKPGTYPIWGST 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 681 YFRWWLADRLHQIAPAYLLSGSSLYNGYLRAMGARIGADVVIGSVTVRQPHLLRIGDGASLGSNVNLENVRVERGELVVG 760
Cdd:TIGR02353 80 YLRFWTVKRLVDAAPTVLLSGSPLYSLYLRALGAKIGKGVDIGSLPPVCTDLLTIGAGTIVRKEVMLLGYRAERGRLHTG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 761 AIEIGREAYVGSYAVMEGDSAIADHGRLEGLAALARGGRIGAREVWDGAPAQR----QRLWSPGqrqpRPDVTRLRRTGE 836
Cdd:TIGR02353 160 PVTLGRDAFIGTRSTLDIDTSIGDGAQLGHGSALQGGQSIPDGERWHGSPAQKtgadYRKVQPA----RPYTVRRRLYVA 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 837 ALFFSLGAALT--SVLFFLPIFPAFVLIDAIEARLqehsievhgkLFAVLQYALYALPASALLIVGTALIAAAIRWLVLP 914
Cdd:TIGR02353 236 GALFVVFVLLPplAFLFAIPVAITFDEIDWTLGPD----------MVGFILALVLTFVALAGFIAYTVLLLAAVRLLLNL 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 915 RLTPGSWPVHSNMYCRKWLANQIQVASLHVLHGVYATVYAPFWYRLLGARIGRNAEISTAMGLIPDMLTLGDDCFIADGV 994
Cdd:TIGR02353 306 VLKPGRYYVHSGFYYQAWTVQQLMDNSRVLLFPLYASSYIPHWYRALGAKIGKVAEISSAQHEVPDLTDIGEETFIADGL 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 995 MLGDEEVDGGWMSVDYTVIGPRSFVGNGAYVPDGTTLPHDVLIGVQSKAPATEQMQPGQTWVGSPALELPARETLHGFPD 1074
Cdd:TIGR02353 386 LMGNARLSGGWFRLGRTRIGRRSFLGNSGYYPPGAKTGDNVLLGVLSMTPKDGKVREGVGWLGSPPFELPRRVNRDDELE 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1075 ALTFRPSPWRRLGRALVEAARIVLPLSLVIGAG--YVIVGAEMPVAAEGDWLRLAFELGLAGIYYGIGCFLFVVALKWLL 1152
Cdd:TIGR02353 466 ALTFEPDPRRRLARKNVENLRIILPFLLVQWAMlfALVVLDLQALDDYTEWGAVALLAALILMAVGVGAFLILVERKWLV 545
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1153 IGRYRPRAAPMWTPFVWISEAVTSVYESIAVPNCLEYLRGTPMLPVALRLLGVRLGRGVYLDSTDFTEFDCVSIGDESEL 1232
Cdd:TIGR02353 546 FGRLKPQEHPLWSPFVWLHELHWKLYESVAVPNFLRPFRGTPFLPAILRLLGVKIGRGVYIDGTDLTERDLVTIGDDSTL 625
|
650 660 670 680 690 700 710
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1233 NAWSGPQTHLFEDRIMKIGRIRIGNRVTLGTRSTVLYDTALGDQVQVGPLSLIMKGESLPARSSWTGSPA 1302
Cdd:TIGR02353 626 NEGSVIQTHLFEDRVMKSDTVTIGDGATLGPGAIVLYGVVMGEGSVLGPDSLVMKGEEVPAHTRWRGNPA 695
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
14-483 |
0e+00 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 542.50 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTGhglregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd05930 81 EDSGAKLVLTD-----------------------------------PDDLAYVIYTSGSTGKPKGVMVEHRGLVNLLLWM 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLALFG-----A 248
Cdd:cd05930 126 QEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRKDPEALADLLAEEGITVLHLTPSLLRLLLqelelA 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 DVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAELRPGD----PVTIGQPLPNYGLLVVDAEMKL 322
Cdd:cd05930 206 ALPSLRLVLVGGEALPPDLVRRWREllPGARLVNLYGPTEATVDATYYRVPPDDeedgRVPIGRPIPNTRVYVLDENLRP 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 323 LPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAgepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELG 402
Cdd:cd05930 286 VPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFGPGE---RMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELG 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 403 EIEAALsRQLPNGTAAVVL--KPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDR 480
Cdd:cd05930 363 EIEAAL-LAHPGVREAAVVarEDGDGEKRLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDR 441
|
...
gi 2772088563 481 KAL 483
Cdd:cd05930 442 KAL 444
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
2-850 |
1.43e-168 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 537.90 E-value: 1.43e-168
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:COG1020 478 LHELFEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLD 557
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIW--DQPELMQKVDGPLLRrgGAGPRHPAYVIYTSGSTGKPKGV 159
Cdd:COG1020 558 PAYPAERLAYMLEDAGARLVLTQSALAARLPELGVPVLalDALALAAEPATNPPV--PVTPDDLAYVIYTSGSTGRPKGV 635
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 160 PINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAV 239
Cdd:COG1020 636 MVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWEIFGALLSGATLVLAPPEARRDPAALAELLARHRVTVLNLT 715
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 240 PTLLALF----GADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAELRPGD----PVTIGQPLP 309
Cdd:COG1020 716 PSLLRALldaaPEALPSLRLVLVGGEALPPELVRRWRArlPGARLVNLYGPTETTVDSTYYEVTPPDadggSVPIGRPIA 795
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 310 NYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEhAGEpRLYRTGDLARIDEQGQIHCLGRAD 389
Cdd:COG1020 796 NTRVYVLDAHLQPVPVGVPGELYIGGAGLARGYLNRPELTAERFVADPFGF-PGA-RLYRTGDLARWLPDGNLEFLGRAD 873
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 390 DQVKVRGFRVELGEIEAALsRQLPNGTAAVVL--KPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELV 467
Cdd:COG1020 874 DQVKIRGFRIELGEIEAAL-LQHPGVREAVVVarEDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLL 952
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 468 DTIPRLTSGKIDRKALrawpmAALSATERSESDLPETEAETALFAALARFFPGLPIQRGGDFFDDLGGHSLLAARLVSAL 547
Cdd:COG1020 953 LPLPLTGNGKLDRLAL-----PAPAAAAAAAAAAPPAEEEEEEAALALLLLLVVVVGDDDFFFFGGGLGLLLLLALARAA 1027
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 548 RedgryagmtvndvygQRRVGAIAAAMEAGQARVAAEPMVFATVPRPRRWLCGLAQALTLPALIALHIMNWLAPFFTYHY 627
Cdd:COG1020 1028 R---------------LLLLLLLLLLLFLAAAAAAAAAAAAAAAAAAAAPLAAAAAPLPLPPLLLSLLALLLALLLLLAL 1092
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 628 YTGEPEDGVAFAVLASVLVFLASHLVSLGIAMSGSRLLLSGLKAGRYPLWGGNYFRWWLADRLHQIAPAYLLSGsslyng 707
Cdd:COG1020 1093 LALLALLLLLLLLLLLLALLLLLALLLALLAALRARRAVRQEGPRLRLLVALAAALALAALLALLLAAAAAAAE------ 1166
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 708 yLRAMGARIGADVVIGSVTVRQPHLLRIGDGASLGSNVNLENVRVERGELVVGAIEIGREAYVGSYAVMEGDSAIADHGR 787
Cdd:COG1020 1167 -LLAAAALLLLLALLLLALLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLAAAAAALLALALLLALLALAA 1245
|
810 820 830 840 850 860
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 788 LEGLAALARGGRIGAREVWDGAPAQRQRLWSPGQRQPRPDVTRLRRTGEALFFSLGAALTSVL 850
Cdd:COG1020 1246 LLALAALAALAAALLALALALLALALLLLALALLLPALARARAARTARALALLLLLALLLLLA 1308
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
14-483 |
1.25e-149 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 460.22 E-value: 1.25e-149
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTGHGLREGLDAIDCPIWDQPElmQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd12116 81 EDAEPALVLTDDALPDRLPAGLPVLLLALA--AAAAAPAAPRTPVSPDDLAYVIYTSGSTGRPKGVVVSHRNLVNFLHSM 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPT----LLALFGAD 249
Cdd:cd12116 159 RERLGLGPGDRLLAVTTYAFDISLLELLLPLLAGARVVIAPRETQRDPEALARLIEAHSITVMQATPAtwrmLLDAGWQG 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 250 VPSLRLInLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLAELRPGD-PVTIGQPLPNYGLLVVDAEMKLLPAGAT 328
Cdd:cd12116 239 RAGLTAL-CGGEALPPDLAARLLSRVGSLWNLYGPTETTIWSTAARVTAAAgPIPIGRPLANTQVYVLDAALRPVPPGVP 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 329 GELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAAL 408
Cdd:cd12116 318 GELYIGGDGVAQGYLGRPALTAERFVPDPFAGPGS--RLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAAL 395
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 409 SRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd12116 396 AAHPGVAQAAVVVREDGGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
14-483 |
1.55e-146 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 450.94 E-value: 1.55e-146
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd17652 1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTghglregldaidcpiwdQPElmqkvdgpllrrggagprHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd17652 81 ADARPALLLT-----------------TPD------------------NLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQ 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVP-TLLALFGADVPS 252
Cdd:cd17652 126 IAAFDVGPGSRVLQFASPSFDASVWELLMALLAGATLVLAPAEELLPGEPLADLLREHRITHVTLPPaALAALPPDDLPD 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 253 LRLINLGGEICPPALVERWAtPTRRLFNSYGPTEATVSASLAE-LRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGEL 331
Cdd:cd17652 206 LRTLVVAGEACPAELVDRWA-PGRRMINAYGPTETTVCATMAGpLPGGGVPPIGRPVPGTRVYVLDARLRPVPPGVPGEL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 332 CIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQ 411
Cdd:cd17652 285 YIAGAGLARGYLNRPGLTAERFVADPFGAPGS--RMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEH 362
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 412 LPNGTAAVVLKPVD-GIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd17652 363 PGVAEAVVVVRDDRpGDKRLVAYVVPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
4-483 |
1.75e-143 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 444.72 E-value: 1.75e-143
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd12117 1 ELFEEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLlrRGGAGPRHPAYVIYTSGSTGKPKGVPINQ 163
Cdd:cd12117 81 LPAERLAFMLADAGAKVLLTDRSLAGRAGGLEVAVVIDEALDAGPAGNP--AVPVSPDDLAYVMYTSGSTGRPKGVAVTH 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 164 GAICHFLRSENeRLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLL 243
Cdd:cd12117 159 RGVVRLVKNTN-YVTLGPDDRVLQTSPLAFDASTFEIWGALLNGARLVLAPKGTLLDPDALGALIAEEGVTVLWLTAALF 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALFGADVP----SLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPGDPVT----IGQPLPNYGL 313
Cdd:cd12117 238 NQLADEDPecfaGLRELLTGGEVVSPPHVRRVlaACPGLRLVNGYGPTENTTFTTSHVVTELDEVAgsipIGRPIANTRV 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 314 LVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADDQVK 393
Cdd:cd12117 318 YVLDEDGRPVPPGVPGELYVGGDGLALGYLNRPALTAERFVADPFG--PGE-RLYRTGDLARWLPDGRLEFLGRIDDQVK 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 394 VRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGID-QLVAYIVPGGgqPVQIAQLRAQLRETLPPYMVPSRFELVDTIPR 472
Cdd:cd12117 395 IRGFRIELGEIEAALRAHPGVREAVVVVREDAGGDkRLVAYVVAEG--ALDAAELRAFLRERLPAYMVPAAFVVLDELPL 472
|
490
....*....|.
gi 2772088563 473 LTSGKIDRKAL 483
Cdd:cd12117 473 TANGKVDRRAL 483
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
27-420 |
2.03e-143 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 441.70 E-value: 2.03e-143
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGH 105
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 GLREGLDAIDCPI--WDQPELMQKVDGPL--LRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRA 181
Cdd:TIGR01733 81 ALASRLAGLVLPVilLDPLELAALDDAPAppPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRYGLDP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 182 DDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQG-ITVLHAVPTLLALFGA----DVPSLRLI 256
Cdd:TIGR01733 161 DDRVLQFASLSFDASVEEIFGALLAGATLVVPPEDEERDDAALLAALIAEHpVTVLNLTPSLLALLAAalppALASLRLV 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 257 NLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAELRPGD-----PVTIGQPLPNYGLLVVDAEMKLLPAGATG 329
Cdd:TIGR01733 241 ILGGEALTPALVDRWRArgPGARLINLYGPTETTVWSTATLVDPDDapresPVPIGRPLANTRLYVLDDDLRPVPVGVVG 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 330 ELCIIGPGVAEGYLGRPELTAEKFLPNPWAeHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALs 409
Cdd:TIGR01733 321 ELYIGGPGVARGYLNRPELTAERFVPDPFA-GGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAAL- 398
|
410
....*....|.
gi 2772088563 410 RQLPNGTAAVV 420
Cdd:TIGR01733 399 LRHPGVREAVV 409
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
6-484 |
3.07e-141 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 439.09 E-value: 3.07e-141
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 6 FEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTP 85
Cdd:cd17651 1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 86 AERVMVCLEDAQAVGIVTGHGLREGLDAIDCPI--WDQPELMQKVDGPllRRGGAGPRHPAYVIYTSGSTGKPKGVPINQ 163
Cdd:cd17651 81 AERLAFMLADAGPVLVLTHPALAGELAVELVAVtlLDQPGAAAGADAE--PDPALDADDLAYVIYTSGSTGRPKGVVMPH 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 164 GAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGIT-------VL 236
Cdd:cd17651 159 RSLANLVAWQARASSLGPGARTLQFAGLGFDVSVQEIFSTLCAGATLVLPPEEVRTDPPALAAWLDEQRISrvflptvAL 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 237 HAVPTLLALFGADVPSLRLINLGGE--ICPPALVERWAT-PTRRLFNSYGPTEATVSASLA----ELRPGDPVTIGQPLP 309
Cdd:cd17651 239 RALAEHGRPLGVRLAALRYLLTGGEqlVLTEDLREFCAGlPGLRLHNHYGPTETHVVTALSlpgdPAAWPAPPPIGRPID 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 310 NYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehAGEPRLYRTGDLARIDEQGQIHCLGRAD 389
Cdd:cd17651 319 NTRVYVLDAALRPVPPGVPGELYIGGAGLARGYLNRPELTAERFVPDPF---VPGARMYRTGDLARWLPDGELEFLGRAD 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 390 DQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGID-QLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVD 468
Cdd:cd17651 396 DQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRPGEkRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLD 475
|
490
....*....|....*.
gi 2772088563 469 TIPRLTSGKIDRKALR 484
Cdd:cd17651 476 ALPLTPNGKLDRRALP 491
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
4-483 |
1.21e-139 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 434.78 E-value: 1.21e-139
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd17646 2 ALVAEQAARTPDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPG 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLLRRgGAGPRHPAYVIYTSGSTGKPKGVPINQ 163
Cdd:cd17646 82 YPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEALAAPPATPPLV-PPRPDNLAYVIYTSGSTGRPKGVMVTH 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 164 GAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLL 243
Cdd:cd17646 161 AGIVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGARLVVARPGGHRDPAYLAALIREHGVTTCHFVPSML 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALF-----GADVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEATVSASLAELRPGD---PVTIGQPLPNYGLL 314
Cdd:cd17646 241 RVFlaepaAGSCASLRRVFCSGEALPPELAARFlALPGAELHNLYGPTEAAIDVTHWPVRGPAetpSVPIGRPVPNTRLY 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 315 VVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:cd17646 321 VLDDALRPVPVGVPGELYLGGVQLARGYLGRPALTAERFVPDPFG--PGS-RMYRTGDLARWRPDGALEFLGRSDDQVKI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALsRQLPNGTAAVVL--KPVDGIDQLVAYIVP-GGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIP 471
Cdd:cd17646 398 RGFRVEPGEIEAAL-AAHPAVTHAVVVarAAPAGAARLVGYVVPaAGAAGPDTAALRAHLAERLPEYMVPAAFVVLDALP 476
|
490
....*....|..
gi 2772088563 472 RLTSGKIDRKAL 483
Cdd:cd17646 477 LTANGKLDRAAL 488
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
4-486 |
4.29e-139 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 432.74 E-value: 4.29e-139
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd05918 3 DLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAFVPLDPS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TPAERVMVCLEDAQAVGIVTGHglregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQ 163
Cdd:cd05918 83 HPLQRLQEILQDTGAKVVLTSS----------------------------------PSDAAYVIFTSGSTGKPKGVVIEH 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 164 GAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITadPDALPLALARQGITVLHAVPTLL 243
Cdd:cd05918 129 RALSTSALAHGRALGLTSESRVLQFASYTFDVSILEIFTTLAAGGCLCIPSEEDR--LNDLAGFINRLRVTWAFLTPSVA 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALFG-ADVPSLRLINLGGEICPPALVERWAtPTRRLFNSYGPTEATVSASLAELRPG-DPVTIGQPLPNyGLLVVDAE-- 319
Cdd:cd05918 207 RLLDpEDVPSLRTLVLGGEALTQSDVDTWA-DRVRLINAYGPAECTIAATVSPVVPStDPRNIGRPLGA-TCWVVDPDnh 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 320 MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNP-WAEHAGEP---RLYRTGDLARIDEQGQIHCLGRADDQVKVR 395
Cdd:cd05918 285 DRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPaWLKQEGSGrgrRLYRTGDLVRYNPDGSLEYVGRKDTQVKIR 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 396 GFRVELGEIEAALSRQLPNGTAAVV--LKPVDG--IDQLVAYIVPGGGQP-----------------VQIAQLRAQLRET 454
Cdd:cd05918 365 GQRVELGEIEHHLRQSLPGAKEVVVevVKPKDGssSPQLVAFVVLDGSSSgsgdgdslflepsdefrALVAELRSKLRQR 444
|
490 500 510
....*....|....*....|....*....|..
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:cd05918 445 LPSYMVPSVFLPLSHLPLTASGKIDRRALREL 476
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
14-483 |
4.34e-138 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 429.04 E-value: 4.34e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd17643 1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTghglregldaidcpiwdQPElmqkvdgpllrrggagprHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd17643 81 ADSGPSLLLT-----------------DPD------------------DLAYVIYTSGSTGRPKGVVVSHANVLALFAAT 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPT-------LLALF 246
Cdd:cd17643 126 QRWFGFNEDDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPYEVARSPEDFARLLRDEGVTVLNQTPSafyqlveAADRD 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 GADVPSLRLINLGGEICPPALVERWA----TPTRRLFNSYGPTEATVSASL-----AELRPGDPVTIGQPLPNYGLLVVD 317
Cdd:cd17643 206 GRDPLALRYVIFGGEALEAAMLRPWAgrfgLDRPQLVNMYGITETTVHVTFrpldaADLPAAAASPIGRPLPGLRVYVLD 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 318 AEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPwaehAGEP--RLYRTGDLARIDEQGQIHCLGRADDQVKVR 395
Cdd:cd17643 286 ADGRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANP----FGGPgsRMYRTGDLARRLPDGELEYLGRADEQVKIR 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 396 GFRVELGEIEAALsRQLPNGTAAVVL--KPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRL 473
Cdd:cd17643 362 GFRIELGEIEAAL-ATHPSVRDAAVIvrEDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLT 440
|
490
....*....|
gi 2772088563 474 TSGKIDRKAL 483
Cdd:cd17643 441 VNGKLDRAAL 450
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
4-484 |
2.90e-135 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 420.95 E-value: 2.90e-135
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd17653 1 DAFERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TPAERVMVCLEDAQAVGIVTGhglregldaidcpiwdqpelmqkvdgpllrrggAGPRHPAYVIYTSGSTGKPKGVPINQ 163
Cdd:cd17653 81 LPSARIQAILRTSGATLLLTT---------------------------------DSPDDLAYIIFTSGSTGIPKGVMVPH 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 164 GAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLwiapreITADPDAlPLALARQGITVLHAVPTLL 243
Cdd:cd17653 128 RGVLNYVSQPPARLDVGPGSRVAQVLSIAFDACIGEIFSTLCNGGTL------VLADPSD-PFAHVARTVDALMSTPSIL 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 A-LFGADVPSLRLINLGGEICPPALVERWAtPTRRLFNSYGPTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKL 322
Cdd:cd17653 201 StLSPQDFPNLKTIFLGGEAVPPSLLDRWS-PGRRLYNAYGPTECTISSTMTELLPGQPVTIGKPIPNSTCYILDADLQP 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 323 LPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaeHAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELG 402
Cdd:cd17653 280 VPEGVVGEICISGVQVARGYLGNPALTASKFVPDPF--WPGS-RMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLE 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 403 EIEAALSRQLPNGTAAVVLkpVDGiDQLVAYIVPgggQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKA 482
Cdd:cd17653 357 EIEEVVLQSQPEVTQAAAI--VVN-GRLVAFVTP---ETVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKA 430
|
..
gi 2772088563 483 LR 484
Cdd:cd17653 431 LR 432
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
14-484 |
3.50e-134 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 418.70 E-value: 3.50e-134
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd17649 1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAqAVGIVTGHGlregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd17649 81 EDS-GAGLLLTHH---------------------------------PRQLAYVIYTSGSTGTPKGVAVSHGPLAAHCQAT 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLALFGADV--- 250
Cdd:cd17649 127 AERYGLTPGDRELQFASFNFDGAHEQLLPPLICGACVVLRPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEAdrt 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 251 -----PSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLAELRPGDP-----VTIGQPLPNYGLLVVDAEM 320
Cdd:cd17649 207 gdgrpPSLRLYIFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVWKCEAGAAragasMPIGRPLGGRSAYILDADL 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 321 KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVE 400
Cdd:cd17649 287 NPVPVGVTGELYIGGEGLARGYLGRPELTAERFVPDPFGAPGS--RLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIE 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 401 LGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGG--QPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:cd17649 365 LGEIEAALLEHPGVREAAVVALDGAGGKQLVAYVVLRAAaaQPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKL 444
|
....*.
gi 2772088563 479 DRKALR 484
Cdd:cd17649 445 DRKALP 450
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
2-483 |
9.52e-132 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 412.10 E-value: 9.52e-132
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:cd12115 1 LHDLVEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGhglregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPI 161
Cdd:cd12115 81 PAYPPERLRFILEDAQARLVLTD-----------------------------------PDDLAYVIYTSGSTGRPKGVAI 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 162 NQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIApreitADPDALPLALARQGITVLHAVPT 241
Cdd:cd12115 126 EHRNAAAFLQWAAAAFSAEELAGVLASTSICFDLSVFELFGPLATGGKVVLA-----DNVLALPDLPAAAEVTLINTVPS 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 242 LL-ALFGADV--PSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPGD--PVTIGQPLPNYGLL 314
Cdd:cd12115 201 AAaELLRHDAlpASVRVVNLAGEPLPRDLVQRLyaRLQVERVVNLYGPSEDTTYSTVAPVPPGAsgEVSIGRPLANTQAY 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 315 VVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPwaEHAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:cd12115 281 VLDRALQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDP--FGPGA-RLYRTGDLVRWRPDGLLEFLGRADNQVKV 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALsRQLPNGTAAVVLKPVDGI--DQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPR 472
Cdd:cd12115 358 RGFRIELGEIEAAL-RSIPGVREAVVVAIGDAAgeRRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPL 436
|
490
....*....|.
gi 2772088563 473 LTSGKIDRKAL 483
Cdd:cd12115 437 TPNGKIDRSAL 447
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
5-483 |
1.85e-131 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 412.88 E-value: 1.85e-131
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:cd17655 2 LFEEQAEKTPDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLLRRGGAgPRHPAYVIYTSGSTGKPKGVPINQG 164
Cdd:cd17655 82 PEERIQYILEDSGADILLTQSHLQPPIAFIGLIDLLDEDTIYHEESENLEPVSK-SDDLAYVIYTSGSTGKPKGVMIEHR 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 165 AICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLA 244
Cdd:cd17655 161 GVVNLVEWANKVIYQGEHLRVALFASISFDASVTEIFASLLSGNTLYIVRKETVLDGQALTQYIRQNRITIIDLTPAHLK 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 LF----GADVPSLRLINLGGEICPPALVERW---ATPTRRLFNSYGPTEATVSASLAELRPGDP----VTIGQPLPNYGL 313
Cdd:cd17655 241 LLdaadDSEGLSLKHLIVGGEALSTELAKKIielFGTNPTITNAYGPTETTVDASIYQYEPETDqqvsVPIGKPLGNTRI 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 314 LVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADDQVK 393
Cdd:cd17655 321 YILDQYGRPQPVGVAGELYIGGEGVARGYLNRPELTAEKFVDDPFV--PGE-RMYRTGDLARWLPDGNIEFLGRIDHQVK 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 394 VRGFRVELGEIEAALSrQLPNGTAAVVL--KPVDGIDQLVAYIVpgGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIP 471
Cdd:cd17655 398 IRGYRIELGEIEARLL-QHPDIKEAVVIarKDEQGQNYLCAYIV--SEKELPVAQLREFLARELPDYMIPSYFIKLDEIP 474
|
490
....*....|..
gi 2772088563 472 RLTSGKIDRKAL 483
Cdd:cd17655 475 LTPNGKVDRKAL 486
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
2-584 |
1.54e-130 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 434.09 E-value: 1.54e-130
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK10252 460 LSALVAQQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLD 539
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKV-DGPLLRRGGagPRHPAYVIYTSGSTGKPKGVP 160
Cdd:PRK10252 540 TGYPDDRLKMMLEDARPSLLITTADQLPRFADVPDLTSLCYNAPLAPqGAAPLQLSQ--PHHTAYIIFTSGSTGRPKGVM 617
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 161 INQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVP 240
Cdd:PRK10252 618 VGQTAIVNRLLWMQNHYPLTADDVVLQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVP 697
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 241 TLLALF---------GADVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSAS--------LAELRpGDPV 302
Cdd:PRK10252 698 SMLAAFvasltpegaRQSCASLRQVFCSGEALPADLCREWQQLTGaPLHNLYGPTEAAVDVSwypafgeeLAAVR-GSSV 776
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 303 TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQI 382
Cdd:PRK10252 777 PIGYPVWNTGLRILDARMRPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFA--PGE-RMYRTGDVARWLDDGAV 853
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 383 HCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLKPV--------DGIDQLVAYIVPGGGQPVQIAQLRAQLRET 454
Cdd:PRK10252 854 EYLGRSDDQLKIRGQRIELGEIDRAM-QALPDVEQAVTHACVinqaaatgGDARQLVGYLVSQSGLPLDTSALQAQLRER 932
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALrawPMAALSAteRSESDLPETEAETALFAALARFFpGLPIQRGGDFFDDLG 534
Cdd:PRK10252 933 LPPHMVPVVLLQLDQLPLSANGKLDRKAL---PLPELKA--QVPGRAPKTGTETIIAAAFSSLL-GCDVVDADADFFALG 1006
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 2772088563 535 GHSLLAARLVSALREDGRYAgMTVNDVYGQRRVGAIAAAMEAGQARVAAE 584
Cdd:PRK10252 1007 GHSLLAMKLAAQLSRQFARQ-VTPGQVMVASTVAKLATLLDAEEDESRRL 1055
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
5-597 |
8.53e-127 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 434.97 E-value: 8.53e-127
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:PRK12467 517 LIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEY 596
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAqAVGIVTGHglREGLDAIDCPIwDQPELMQKVDGPLLRRGGA-------GPRHPAYVIYTSGSTGKPK 157
Cdd:PRK12467 597 PQDRLAYMLDDS-GVRLLLTQ--SHLLAQLPVPA-GLRSLCLDEPADLLCGYSGhnpevalDPDNLAYVIYTSGSTGQPK 672
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 158 GVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLH 237
Cdd:PRK12467 673 GVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGATLHLLPPDCARDAEAFAALMADQGVTVLK 752
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 238 AVPTLLALF-----GADVPSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAEL----RPGDPVTIGQ 306
Cdd:PRK12467 753 IVPSHLQALlqasrVALPRPQRALVCGGEALQVDLLARVraLGPGARLINHYGPTETTVGVSTYELsdeeRDFGNVPIGQ 832
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 307 PLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLG 386
Cdd:PRK12467 833 PLANLGLYILDHYLNPVPVGVVGELYIGGAGLARGYHRRPALTAERFVPDPFGADGG--RLYRTGDLARYRADGVIEYLG 910
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 387 RADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVP-----GGGQPVQIAQLRAQLRETLPPYMVP 461
Cdd:PRK12467 911 RMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLVAYLVPaavadGAEHQATRDELKAQLRQVLPDYMVP 990
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 462 SRFELVDTIPRLTSGKIDRKALRAWPMAALSATERSesdlPETEAETALFAALARFFPGLPIQRGGDFFdDLGGHSLLAA 541
Cdd:PRK12467 991 AHLLLLDSLPLTPNGKLDRKALPKPDASAVQATFVA----PQTELEKRLAAIWADVLKVERVGLTDNFF-ELGGHSLLAT 1065
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 542 RLVSALRED-GRYagMTVNDVYGQRRVGAIAAAMEAGQ----------ARVAAEPMVFAtvpRPRRW 597
Cdd:PRK12467 1066 QVISRVRQRlGIQ--VPLRTLFEHQTLAGFAQAVAAQQqgaqpalpdvDRDQPLPLSYA---QERQW 1127
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
10-483 |
5.09e-124 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 391.61 E-value: 5.09e-124
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERV 89
Cdd:cd05945 1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 90 MVCLEDAQ-AVGIVTGHGLregldaidcpiwdqpelmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQGAICH 168
Cdd:cd05945 81 REILDAAKpALLIADGDDN------------------------------------AYIIFTSGSTGRPKGVQISHDNLVS 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 169 FLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLAL--- 245
Cdd:cd05945 125 FTNWMLSDFPLGPGDVFLNQAPFSFDLSVMDLYPALASGATLVPVPRDATADPKQLFRFLAEHGITVWVSTPSFAAMcll 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 246 ---FGAD-VPSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPG-----DPVTIGQPLPNYGLL 314
Cdd:cd05945 205 sptFTPEsLPSLRHFLFCGEVLPHKTARALqqRFPDARIYNTYGPTEATVAVTYIEVTPEvldgyDRLPIGYAKPGAKLV 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 315 VVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPwaehaGEPRlYRTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:cd05945 285 ILDEDGRPVPPGEKGELVISGPSVSKGYLNNPEKTAAAFFPDE-----GQRA-YRTGDLVRLEADGLLFYRGRLDFQVKL 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALsRQLPNGTAAVVL-KPV-DGIDQLVAYIVPG-GGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIP 471
Cdd:cd05945 359 NGYRIELEEIEAAL-RQVPGVKEAVVVpKYKgEKVTELIAFVVPKpGAEAGLTKAIKAELAERLPPYMIPRRFVYLDELP 437
|
490
....*....|..
gi 2772088563 472 RLTSGKIDRKAL 483
Cdd:cd05945 438 LNANGKIDRKAL 449
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
5-592 |
7.34e-119 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 411.27 E-value: 7.34e-119
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:PRK12316 516 LFEEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEY 595
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTGHGLREGLD---AIDCPIWDQP--ELMQKVDGPLLRRggAGPRHPAYVIYTSGSTGKPKGV 159
Cdd:PRK12316 596 PAERLAYMLEDSGVQLLLSQSHLGRKLPlaaGVQVLDLDRPaaWLEGYSEENPGTE--LNPENLAYVIYTSGSTGKPKGA 673
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 160 PINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAV 239
Cdd:PRK12316 674 GNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGARLVVAAPGDHRDPAKLVELINREGVDTLHFV 753
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 240 PTLLALF-----GADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAELRP--GDPVTIGQPLPN 310
Cdd:PRK12316 754 PSMLQAFlqdedVASCTSLRRIVCSGEALPADAQEQVFAklPQAGLYNLYGPTEAAIDVTHWTCVEegGDSVPIGRPIAN 833
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 311 YGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADD 390
Cdd:PRK12316 834 LACYILDANLEPVPVGVLGELYLAGRGLARGYHGRPGLTAERFVPSPFV--AGE-RMYRTGDLARYRADGVIEYAGRIDH 910
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 391 QVKVRGFRVELGEIEAALsRQLPNGTAAVVLKpVDGiDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTI 470
Cdd:PRK12316 911 QVKLRGLRIELGEIEARL-LEHPWVREAAVLA-VDG-KQLVGYVVLESEGGDWREALKAHLAASLPEYMVPAQWLALERL 987
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 471 PRLTSGKIDRKALRAwPMAALSATERSEsdlPETEAETALFAALARFfpgLPIQRGG--DFFDDLGGHSLLAARLVSALR 548
Cdd:PRK12316 988 PLTPNGKLDRKALPA-PEASVAQQGYVA---PRNALERTLAAIWQDV---LGVERVGldDNFFELGGDSIVSIQVVSRAR 1060
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 2772088563 549 EDGryAGMTVNDVYGQRRVGAIAAAMEAGQARVAAEPMVFATVP 592
Cdd:PRK12316 1061 QAG--IQLSPRDLFQHQTIRSLALVAKAGQATAADQGPASGEVA 1102
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
2-494 |
7.37e-118 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 375.30 E-value: 7.37e-118
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:COG0318 1 LADLLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTghglregldaidcpiwdqpelmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPI 161
Cdd:COG0318 81 PRLTAEELAYILEDSGARALVT----------------------------------------ALILYTSGTTGRPKGVML 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 162 NQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYL-VGATLWIAPReitADPDALPLALARQGITVLHAVP 240
Cdd:COG0318 121 THRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLlAGATLVLLPR---FDPERVLELIERERVTVLFGVP 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 241 TLLA-------LFGADVPSLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEAT--VSASLAELRPGDPVTIGQPLPN 310
Cdd:COG0318 198 TMLArllrhpeFARYDLSSLRLVVSGGAPLPPELLERFEERFgVRIVEGYGLTETSpvVTVNPEDPGERRPGSVGRPLPG 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 311 YGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADD 390
Cdd:COG0318 278 VEVRIVDEDGRELPPGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGW---------LRTGDLGRLDEDGYLYIVGRKKD 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 391 QVKVRGFRVELGEIEAALsRQLPNGT-AAVVLKPVDGIDQ-LVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVD 468
Cdd:COG0318 348 MIISGGENVYPAEVEEVL-AAHPGVAeAAVVGVPDEKWGErVVAFVVLRPGAELDAEELRAFLRERLARYKVPRRVEFVD 426
|
490 500
....*....|....*....|....*.
gi 2772088563 469 TIPRLTSGKIDRKALRAWPMAALSAT 494
Cdd:COG0318 427 ELPRTASGKIDRRALRERYAAGALEA 452
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
5-483 |
3.00e-116 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 371.38 E-value: 3.00e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:cd17644 5 LFEEQVERTPDAVAVVFEDQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNY 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTghglregldaidcpiwdQPELMqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQG 164
Cdd:cd17644 85 PQERLTYILEDAQISVLLT-----------------QPENL------------------AYVIYTSGSTGKPKGVMIEHQ 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 165 AICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLA 244
Cdd:cd17644 130 SLVNLSHGLIKEYGITSSDRVLQFASIAFDVAAEEIYVTLLSGATLVLRPEEMRSSLEDFVQYIQQWQLTVLSLPPAYWH 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 LF-------GADVP-SLRLINLGGEICPPALVERWATPTR---RLFNSYGPTEATVSASLAEL-----RPGDPVTIGQPL 308
Cdd:cd17644 210 LLvlelllsTIDLPsSLRLVIVGGEAVQPELVRQWQKNVGnfiQLINVYGPTEATIAATVCRLtqlteRNITSVPIGRPI 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 309 PNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGEpRLYRTGDLARIDEQGQIHCLGRA 388
Cdd:cd17644 290 ANTQVYILDENLQPVPVGVPGELHIGGVGLARGYLNRPELTAEKFISHPFNSSESE-RLYKTGDLARYLPDGNIEYLGRI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 389 DDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLKPVD--GIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFEL 466
Cdd:cd17644 369 DNQVKIRGFRIELGEIEAVLS-QHNDVKTAVVIVREDqpGNKRLVAYIVPHYEESPSTVELRQFLKAKLPDYMIPSAFVV 447
|
490
....*....|....*..
gi 2772088563 467 VDTIPRLTSGKIDRKAL 483
Cdd:cd17644 448 LEELPLTPNGKIDRRAL 464
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
5-584 |
1.95e-113 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 394.53 E-value: 1.95e-113
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:PRK12467 1579 LIEDQAAATPEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEY 1658
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTGHGLREGLDAID---CPIWDQPELMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPI 161
Cdd:PRK12467 1659 PRERLAYMIEDSGIELLLTQSHLQARLPLPDglrSLVLDQEDDWLEGYSDSNPAVNLAPQNLAYVIYTSGSTGRPKGAGN 1738
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 162 NQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPT 241
Cdd:PRK12467 1739 RHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLINGARLVIAPPGAHRDPEQLIQLIERQQVTTLHFVPS 1818
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 242 LLALFGADVP------SLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPGDP-----VTIGQPL 308
Cdd:PRK12467 1819 MLQQLLQMDEqvehplSLRRVVCGGEALEVEALRPWleRLPDTGLFNLYGPTETAVDVTHWTCRRKDLegrdsVPIGQPI 1898
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 309 PNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLGRA 388
Cdd:PRK12467 1899 ANLSTYILDASLNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFGTVGS--RLYRTGDLARYRADGVIEYLGRI 1976
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 389 DDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQI--------AQLRAQLRETLPPYMV 460
Cdd:PRK12467 1977 DHQVKIRGFRIELGEIEARLREQGGVREAVVIAQDGANGKQLVAYVVPTDPGLVDDdeaqvalrAILKNHLKASLPEYMV 2056
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 461 PSRFELVDTIPRLTSGKIDRKALRAwPMAALSATERSEsdlPETEAETALfAALARFFPGLP-IQRGGDFFdDLGGHSLL 539
Cdd:PRK12467 2057 PAHLVFLARMPLTPNGKLDRKALPA-PDASELQQAYVA---PQSELEQRL-AAIWQDVLGLEqVGLHDNFF-ELGGDSII 2130
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 2772088563 540 AARLVSALREDGryAGMTVNDVYGQRRVGAIAAAMEAGQARVAAE 584
Cdd:PRK12467 2131 SIQVVSRARQAG--IRFTPKDLFQHQTVQSLAAVAQEGDGTVSID 2173
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
2-582 |
2.45e-113 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 394.32 E-value: 2.45e-113
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK12316 2005 VHQRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLD 2084
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLD--------AIDCPIW--DQPELMQKVDgpllrrggAGPRHPAYVIYTSG 151
Cdd:PRK12316 2085 PNYPAERLAYMLEDSGAALLLTQRHLLERLPlpagvarlPLDRDAEwaDYPDTAPAVQ--------LAGENLAYVIYTSG 2156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 152 STGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITaDPDALPLALARQ 231
Cdd:PRK12316 2157 STGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSFDGAHEQWFHPLLNGARVLIRDDELW-DPEQLYDEMERH 2235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 232 GITVLHAVPTLLALFGADV------PSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPGDP-- 301
Cdd:PRK12316 2236 GVTILDFPPVYLQQLAEHAerdgrpPAVRVYCFGGEAVPAASLRLAweALRPVYLFNGYGPTEAVVTPLLWKCRPQDPcg 2315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 302 ---VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAeHAGEpRLYRTGDLARIDE 378
Cdd:PRK12316 2316 aayVPIGRALGNRRAYILDADLNLLAPGMAGELYLGGEGLARGYLNRPGLTAERFVPDPFS-ASGE-RLYRTGDLARYRA 2393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 379 QGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPY 458
Cdd:PRK12316 2394 DGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQDGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAY 2473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 459 MVPSRFELVDTIPRLTSGKIDRKALRAWPMAALsateRSESDLPETEAETALFAALARFFPGLPIQRGGDFFdDLGGHSL 538
Cdd:PRK12316 2474 MVPAHWVVLERLPLNPNGKLDRKALPKPDVSQL----RQAYVAPQEGLEQRLAAIWQAVLKVEQVGLDDHFF-ELGGHSL 2548
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 2772088563 539 LAARLVSALREDGRYAgMTVNDVYGQRRVGAIAAAMEAGQARVA 582
Cdd:PRK12316 2549 LATQVVSRVRQDLGLE-VPLRILFERPTLAAFAASLESGQTSRA 2591
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
1-550 |
3.30e-112 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 390.86 E-value: 3.30e-112
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPF 80
Cdd:PRK12316 4552 CVHQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPL 4631
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADTPAERVMVCLEDAQAVGIVTGHGLREGL---DAIDCPIWDQPELMQ--KVDGPLLRrggAGPRHPAYVIYTSGSTGK 155
Cdd:PRK12316 4632 DPEYPRERLAYMMEDSGAALLLTQSHLLQRLpipDGLASLALDRDEDWEgfPAHDPAVR---LHPDNLAYVIYTSGSTGR 4708
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 156 PKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITaDPDALPLALARQGITV 235
Cdd:PRK12316 4709 PKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSFDGSHEGLYHPLINGASVVIRDDSLW-DPERLYAEIHEHRVTV 4787
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 236 LHAVPTLLALF------GADVPSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASLAELRPGDP-----V 302
Cdd:PRK12316 4788 LVFPPVYLQQLaehaerDGEPPSLRVYCFGGEAVAQASYDLAwrALKPVYLFNGYGPTETTVTVLLWKARDGDAcgaayM 4867
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 303 TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQI 382
Cdd:PRK12316 4868 PIGTPLGNRSGYVLDGQLNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFGAPGG--RLYRTGDLARYRADGVI 4945
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 383 HCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVP--------GGGQPVQIAQLRAQLRET 454
Cdd:PRK12316 4946 DYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAVGKQLVGYVVPqdpaladaDEAQAELRDELKAALRER 5025
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALSATERSesdlPETEAETALFAALARFfpgLPIQRGG--DFFDD 532
Cdd:PRK12316 5026 LPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASLLQQAYVA----PRSELEQQVAAIWAEV---LQLERVGldDNFFE 5098
|
570
....*....|....*...
gi 2772088563 533 LGGHSLLAARLVSALRED 550
Cdd:PRK12316 5099 LGGHSLLAIQVTSRIQLE 5116
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
14-483 |
3.99e-110 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 354.08 E-value: 3.99e-110
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd17650 1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTghglregldaidcpiwdQPElmqkvdgpllrrggagprHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd17650 81 EDSGAKLLLT-----------------QPE------------------DLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAW 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGV-RADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTL-------LAL 245
Cdd:cd17650 126 RREYELdSFPVRLLQMASFSFDVFAGDFARSLLNGGTLVICPDEVKLDPAALYDLILKSRITLMESTPALirpvmayVYR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 246 FGADVPSLRLINLGGEICPPA----LVERWATPTRrLFNSYGPTEATVSAS-----LAELRPGDPVTIGQPLPNYGLLVV 316
Cdd:cd17650 206 NGLDLSAMRLLIVGSDGCKAQdfktLAARFGQGMR-IINSYGVTEATIDSTyyeegRDPLGDSANVPIGRPLPNTAMYVL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 317 DAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRG 396
Cdd:cd17650 285 DERLQPQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFA--PGE-RMYRTGDLARWRADGNVELLGRVDHQVKIRG 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 397 FRVELGEIEAALsRQLPNGTAAVVLKPVD--GIDQLVAYIVPggGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLT 474
Cdd:cd17650 362 FRIELGEIESQL-ARHPAIDEAVVAVREDkgGEARLCAYVVA--AATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTP 438
|
....*....
gi 2772088563 475 SGKIDRKAL 483
Cdd:cd17650 439 NGKVDRRAL 447
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
14-483 |
6.64e-109 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 350.93 E-value: 6.64e-109
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQG-VRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVC 92
Cdd:cd17648 1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAeIRPDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 LEDAQAVGIVTGhglregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRS 172
Cdd:cd17648 81 LEDTGARVVITN-----------------------------------STDLAYAIYTSGTTGKPKGVLVEHGSVVNLRTS 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 173 ENERLGVRA-DDRVYQGFS-VAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLALFG-AD 249
Cdd:cd17648 126 LSERYFGRDnGDEAVLFFSnYVFDFFVEQMTLALLNGQKLVVPPDEMRFDPDRFYAYINREKVTYLSGTPSVLQQYDlAR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 250 VPSLRLINLGGE-ICPP---ALVERWATPtrrLFNSYGPTEATVSASLAELRPGDPV--TIGQPLPNYGLLVVDAEMKLL 323
Cdd:cd17648 206 LPHLKRVDAAGEeFTAPvfeKLRSRFAGL---IINAYGPTETTVTNHKRFFPGDQRFdkSLGRPVRNTKCYVLNDAMKRV 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 324 PAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEhAGE------PRLYRTGDLARIDEQGQIHCLGRADDQVKVRGF 397
Cdd:cd17648 283 PVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFQT-EQErargrnARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQ 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 398 RVELGEIEAALSrQLPNGTAAVVL-------KPVDGIDQLVAYIVPGGGQpVQIAQLRAQLRETLPPYMVPSRFELVDTI 470
Cdd:cd17648 362 RIEPGEVEAALA-SYPGVRECAVVakedasqAQSRIQKYLVGYYLPEPGH-VPESDLLSFLRAKLPRYMVPARLVRLEGI 439
|
490
....*....|...
gi 2772088563 471 PRLTSGKIDRKAL 483
Cdd:cd17648 440 PVTINGKLDVRAL 452
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
2-582 |
9.68e-109 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 380.28 E-value: 9.68e-109
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK12467 3097 VHQLIEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLD 3176
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDaidcpiwdQPELMQKVDGPLLRRGGAGPRHP---------AYVIYTSGS 152
Cdd:PRK12467 3177 PEYPRERLAYMIEDSGVKLLLTQAHLLEQLP--------APAGDTALTLDRLDLNGYSENNPstrvmgenlAYVIYTSGS 3248
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITaDPDALPLALARQG 232
Cdd:PRK12467 3249 TGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFSFDGAQERFLWTLICGGCLVVRDNDLW-DPEELWQAIHAHR 3327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 233 ITVLHAVPTLLALF-----GADVPSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSASL----AELRPGDP 301
Cdd:PRK12467 3328 ISIACFPPAYLQQFaedagGADCASLDIYVFGGEAVPPAAFEQVkrKLKPRGLTNGYGPTEAVVTVTLwkcgGDAVCEAP 3407
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 302 -VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQG 380
Cdd:PRK12467 3408 yAPIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGVGLARGYHQRPSLTAERFVADPFSGSGG--RLYRTGDLARYRADG 3485
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 381 QIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMV 460
Cdd:PRK12467 3486 VIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLARDGAGGKQLVAYVVPADPQGDWRETLRDHLAASLPDYMV 3565
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 461 PSRFELVDTIPRLTSGKIDRKALrawpmAALSATERSESDLPETEAETALFAALARFfpgLPIQRGG--DFFDDLGGHSL 538
Cdd:PRK12467 3566 PAQLLVLAAMPLGPNGKVDRKAL-----PDPDAKGSREYVAPRSEVEQQLAAIWADV---LGVEQVGvtDNFFELGGDSL 3637
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 2772088563 539 LAARLVSALREDGRYaGMTVNDVYGQRRVGAIAAAMEAGQARVA 582
Cdd:PRK12467 3638 LALQVLSRIRQSLGL-KLSLRDLMSAPTIAELAGYSPLGDVPVN 3680
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
14-483 |
1.36e-108 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 351.19 E-value: 1.36e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd12114 1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLLRRggAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd12114 81 ADAGARLVLTDGPDAQLDVAVFDVLILDLDALAAPAPPPPVD--VAPDDLAYVIFTSGSTGTPKGVMISHRAALNTILDI 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLAL-------F 246
Cdd:cd12114 159 NRRFAVGPDDRVLALSSLSFDLSVYDIFGALSAGATLVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMlldvleaA 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 GADVPSLRLINLGGEICPPALVERWA--TPTRRLFNSYGPTEATVSASLAELRPGDP--VTI--GQPLPNYGLLVVDAEM 320
Cdd:cd12114 239 QALLPSLRLVLLSGDWIPLDLPARLRalAPDARLISLGGATEASIWSIYHPIDEVPPdwRSIpyGRPLANQRYRVLDPRG 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 321 KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpnpwaEHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVE 400
Cdd:cd12114 319 RDCPDWVPGELWIGGRGVALGYLGDPELTAARFV-----THPDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIE 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 401 LGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVP-GGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKID 479
Cdd:cd12114 394 LGEIEAALQAHPGVARAVVVVLGDPGGKRLAAFVVPdNDGTPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVD 473
|
....
gi 2772088563 480 RKAL 483
Cdd:cd12114 474 RAAL 477
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
6-395 |
1.51e-99 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 324.27 E-value: 1.51e-99
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 6 FEATAASQPDQTALVCGAL-SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:pfam00501 1 LERQAARTPDKTALEVGEGrRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTG-----HGLREGLDAIDCPI-------------WDQPELMQKVDGPLLRRGGAGPRHPAYV 146
Cdd:pfam00501 81 PAEELAYILEDSGAKVLITDdalklEELLEALGKLEVVKlvlvldrdpvlkeEPLPEEAKPADVPPPPPPPPDPDDLAYI 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 147 IYTSGSTGKPKGVPINQGAICHFLRS----ENERLGVRADDRVYQGFSVAFDMSFE-EIWISYLVGATLWIAPREITADP 221
Cdd:pfam00501 161 IYTSGTTGKPKGVMLTHRNLVANVLSikrvRPRGFGLGPDDRVLSTLPLFHDFGLSlGLLGPLLAGATVVLPPGFPALDP 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLALARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEATVSASL 293
Cdd:pfam00501 241 AALLELIERYKVTVLYGVPTLLNMLleagapkRALLSSLRLVLSGGAPLPPELARRFrELFGGALVNGYGLTETTGVVTT 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 294 AELRPGDPV---TIGQPLPNYGLLVVDAE-MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYR 369
Cdd:pfam00501 321 PLPLDEDLRslgSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRGPGVMKGYLNDPELTAEAFDEDGW---------YR 391
|
410 420
....*....|....*....|....*.
gi 2772088563 370 TGDLARIDEQGQIHCLGRADDQVKVR 395
Cdd:pfam00501 392 TGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
2-549 |
7.42e-99 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 350.24 E-value: 7.42e-99
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK05691 1133 LPELLNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLD 1212
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDAID--CPI-WDQPELMQ-KVDGPLLRRGGagpRHPAYVIYTSGSTGKPK 157
Cdd:PRK05691 1213 PDYPAERLAYMLADSGVELLLTQSHLLERLPQAEgvSAIaLDSLHLDSwPSQAPGLHLHG---DNLAYVIYTSGSTGQPK 1289
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 158 GVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLH 237
Cdd:PRK05691 1290 GVGNTHAALAERLQWMQATYALDDSDVLMQKAPISFDVSVWECFWPLITGCRLVLAGPGEHRDPQRIAELVQQYGVTTLH 1369
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 238 AVPTLLALF-----GADVPSLRLINLGGEICPPALVER--WATPTRRLFNSYGPTEATVSASLAELRP--GDPVTIGQPL 308
Cdd:PRK05691 1370 FVPPLLQLFideplAAACTSLRRLFSGGEALPAELRNRvlQRLPQVQLHNRYGPTETAINVTHWQCQAedGERSPIGRPL 1449
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 309 PNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEhAGEpRLYRTGDLARIDEQGQIHCLGRA 388
Cdd:PRK05691 1450 GNVLCRVLDAELNLLPPGVAGELCIGGAGLARGYLGRPALTAERFVPDPLGE-DGA-RLYRTGDRARWNADGALEYLGRL 1527
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 389 DDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVD 468
Cdd:PRK05691 1528 DQQVKLRGFRVEPEEIQARLLAQPGVAQAAVLVREGAAGAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLD 1607
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 469 TIPRLTSGKIDRKALrawPMAALSATERSEsdlPETEAETALfAALARFFPGLP-IQRGGDFFdDLGGHSLLAARLVSAL 547
Cdd:PRK05691 1608 QMPLGPSGKLDRRAL---PEPVWQQREHVE---PRTELQQQI-AAIWREVLGLPrVGLRDDFF-ALGGHSLLATQIVSRT 1679
|
..
gi 2772088563 548 RE 549
Cdd:PRK05691 1680 RQ 1681
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
5-594 |
7.50e-98 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 347.33 E-value: 7.50e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:PRK12316 3062 LFEEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEY 3141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTGHGLR----EGLDAIDcpIWDQPELMQKVDGPLLrrggAGPRHPAYVIYTSGSTGKPKGVP 160
Cdd:PRK12316 3142 PEERLAYMLEDSGAQLLLSQSHLRlplaQGVQVLD--LDRGDENYAEANPAIR----TMPENLAYVIYTSGSTGKPKGVG 3215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 161 INQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVP 240
Cdd:PRK12316 3216 IRHSALSNHLCWMQQAYGLGVGDRVLQFTTFSFDVFVEELFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYP 3295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 241 TLLALFGADVP-----SLRLINLGGEICPPALVERWATpTRRLFNSYGPTEATVSASLAELRPGDPVT--IGQPLPNYGL 313
Cdd:PRK12316 3296 SMLQAFLEEEDahrctSLKRIVCGGEALPADLQQQVFA-GLPLYNLYGPTEATITVTHWQCVEEGKDAvpIGRPIANRAC 3374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 314 LVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAgepRLYRTGDLARIDEQGQIHCLGRADDQVK 393
Cdd:PRK12316 3375 YILDGSLEPVPVGALGELYLGGEGLARGYHNRPGLTAERFVPDPFVPGE---RLYRTGDLARYRADGVIEYIGRVDHQVK 3451
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 394 VRGFRVELGEIEAALsRQLPNGTAAVVLKpvDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRL 473
Cdd:PRK12316 3452 IRGFRIELGEIEARL-LEHPWVREAVVLA--VDGRQLVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLT 3528
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 474 TSGKIDRKALRAwPMAALSATERSEsdlPETEAETALFAALARFfpgLPIQRGG--DFFDDLGGHSLLAARLVSALREDG 551
Cdd:PRK12316 3529 PNGKLDRKALPR-PDAALLQQDYVA---PVNELERRLAAIWADV---LKLEQVGltDNFFELGGDSIISLQVVSRARQAG 3601
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 2772088563 552 RYagMTVNDVYGQRRVGAIAAAMEAGQAR-VAAEPMVFATVPRP 594
Cdd:PRK12316 3602 IR--FTPKDLFQHQTIQGLARVARVGGGVaVDQGPVSGETLLLP 3643
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
5-483 |
2.73e-96 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 316.03 E-value: 2.73e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:cd17645 3 LFEEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTghglregldaidcpiwdQPELMqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQG 164
Cdd:cd17645 83 PGERIAYMLADSSAKILLT-----------------NPDDL------------------AYVIYTSGSTGLPKGVMIEHH 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 165 AICHFLRSENERLGVRADDR--VYQGFSvaFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHaVPTL 242
Cdd:cd17645 128 NLVNLCEWHRPYFGVTPADKslVYASFS--FDASAWEIFPHLTAGAALHVVPSERRLDLDALNDYFNQEGITISF-LPTG 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 243 LA--LFGADVPSLRLINLGGEIcppalVERWATPTRRLFNSYGPTEATVSASLAEL-RPGDPVTIGQPLPNYGLLVVDAE 319
Cdd:cd17645 205 AAeqFMQLDNQSLRVLLTGGDK-----LKKIERKGYKLVNNYGPTENTVVATSFEIdKPYANIPIGKPIDNTRVYILDEA 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 320 MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAehAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRV 399
Cdd:cd17645 280 LQLQPIGVAGELCIAGEGLARGYLNRPELTAEKFIVHPFV--PGE-RMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRI 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 400 ELGEIEAALSR-QLPNGTAAVVLKPVDGIDQLVAYIVPggGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:cd17645 357 EPGEIEPFLMNhPLIELAAVLAKEDADGRKYLVAYVTA--PEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKV 434
|
....*
gi 2772088563 479 DRKAL 483
Cdd:cd17645 435 DRKAL 439
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
14-483 |
4.75e-94 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 311.33 E-value: 4.75e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd17656 2 PDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTGHGLREGLD---AIDCPIWDqpeLMQKVDGPLLRRGGAGpRHPAYVIYTSGSTGKPKGVPINQGAICHFL 170
Cdd:cd17656 82 LDSGVRVVLTQRHLKSKLSfnkSTILLEDP---SISQEDTSNIDYINNS-DDLLYIIYTSGTTGKPKGVQLEHKNMVNLL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 171 RSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLAL----- 245
Cdd:cd17656 158 HFEREKTNINFSDKVLQFATCSFDVCYQEIFSTLLSGGTLYIIREETKRDVEQLFDLVKRHNIEVVFLPVAFLKFifser 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 246 -FGADVPS-LRLINLGGE--ICPPALVERWATPTRRLFNSYGPTEATVsASLAELRPGDPVT----IGQPLPNYGLLVVD 317
Cdd:cd17656 238 eFINRFPTcVKHIITAGEqlVITNEFKEMLHEHNVHLHNHYGPSETHV-VTTYTINPEAEIPelppIGKPISNTWIYILD 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 318 AEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAgepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGF 397
Cdd:cd17656 317 QEQQLQPQGIVGELYISGASVARGYLNRQELTAEKFFPDPFDPNE---RMYRTGDLARYLPDGNIEFLGRADHQVKIRGY 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 398 RVELGEIEAALSRQlPNGTAAVVLKPVD--GIDQLVAYIVPggGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTS 475
Cdd:cd17656 394 RIELGEIEAQLLNH-PGVSEAVVLDKADdkGEKYLCAYFVM--EQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPN 470
|
....*...
gi 2772088563 476 GKIDRKAL 483
Cdd:cd17656 471 GKVDRKAL 478
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
2-614 |
3.80e-91 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 326.35 E-value: 3.80e-91
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK05691 2190 LHGLFAAQAARTPQAPALTFAGQTLSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLD 2269
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAqAVGIVTGHglREGLDAI-DCPI----W----DQPELMQKVDGPLLRRggAGPRHPAYVIYTSGS 152
Cdd:PRK05691 2270 PEYPLERLHYMIEDS-GIGLLLSD--RALFEALgELPAgvarWcledDAAALAAYSDAPLPFL--SLPQHQAYLIYTSGS 2344
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLwIAPREITADPDALPLALARQG 232
Cdd:PRK05691 2345 TGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSINFDAASERLLVPLLCGARV-VLRAQGQWGAEEICQLIREQQ 2423
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 233 ITVLHAVPTL-------LALFGADVPsLRLINLGGEICPPALVE--RWATPTRRLFNSYGPTEaTVSASLAELRP----- 298
Cdd:PRK05691 2424 VSILGFTPSYgsqlaqwLAGQGEQLP-VRMCITGGEALTGEHLQriRQAFAPQLFFNAYGPTE-TVVMPLACLAPeqlee 2501
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 299 -GDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGepRLYRTGDLARID 377
Cdd:PRK05691 2502 gAASVPIGRVVGARVAYILDADLALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFAADGG--RLYRTGDLVRLR 2579
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 378 EQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVP--GGGQPVQIAQLR----AQL 451
Cdd:PRK05691 2580 ADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLALDTPSGKQLAGYLVSavAGQDDEAQAALRealkAHL 2659
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 452 RETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALsateRSESDLPETEAETALfAALARffPGLPIQRGG--DF 529
Cdd:PRK05691 2660 KQQLPDYMVPAHLILLDSLPLTANGKLDRRALPAPDPELN----RQAYQAPRSELEQQL-AQIWR--EVLNVERVGlgDN 2732
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 530 FDDLGGHSLLAARLVSALREDGRYagMTVNDVYGQRRVGAIAAAMEAGQARVAAEPMV-------------FAT-VPRPR 595
Cdd:PRK05691 2733 FFELGGDSILSIQVVSRARQLGIH--FSPRDLFQHQTVQTLAAVATHSEAAQAEQGPLqgasgltpiqhwfFDSpVPQPQ 2810
|
650
....*....|....*....
gi 2772088563 596 RWlcglAQALTLPALIALH 614
Cdd:PRK05691 2811 HW----NQALLLEPRQALD 2825
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
3-545 |
9.62e-88 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 315.95 E-value: 9.62e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 3 ADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDA 82
Cdd:PRK05691 3723 VRLFEAQVAAHPQRIAASCLDQQWSYAELNRAANRLGHALRAAGVGVDQPVALLAERGLDLLGMIVGSFKAGAGYLPLDP 3802
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 83 DTPAER-----------VMVCLEDAQAvgivTGHGLREGLDAIDCP---IWDQpelMQKVDGPLLRRG-GAGPRHPAYVI 147
Cdd:PRK05691 3803 GLPAQRlqriielsrtpVLVCSAACRE----QARALLDELGCANRPrllVWEE---VQAGEVASHNPGiYSGPDNLAYVI 3875
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 148 YTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLA 227
Cdd:PRK05691 3876 YTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIVPNAIAHDPQGLLAH 3955
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGITVLHAVPTL----LALFGADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTE-----ATVSASLAEL 296
Cdd:PRK05691 3956 VQAQGITVLESVPSLiqgmLAEDRQALDGLRWMLPTGEAMPPELARQWLQryPQIGLVNAYGPAEcsddvAFFRVDLAST 4035
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 297 RpGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEhAGEpRLYRTGDLARI 376
Cdd:PRK05691 4036 R-GSYLPIGSPTDNNRLYLLDEALELVPLGAVGELCVAGTGVGRGYVGDPLRTALAFVPHPFGA-PGE-RLYRTGDLARR 4112
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 377 DEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV-VLKPVDGiDQLVAYIVPGGG---QPVQIAQLRAQLR 452
Cdd:PRK05691 4113 RSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVaVQEGVNG-KHLVGYLVPHQTvlaQGALLERIKQRLR 4191
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 453 ETLPPYMVPSRFELVDTIPRLTSGKIDRKALrawPMAALSATERSESDLPETEAETALFAALARFfpgLPIQRGG--DFF 530
Cdd:PRK05691 4192 AELPDYMVPLHWLWLDRLPLNANGKLDRKAL---PALDIGQLQSQAYLAPRNELEQTLATIWADV---LKVERVGvhDNF 4265
|
570
....*....|....*
gi 2772088563 531 DDLGGHSLLAARLVS 545
Cdd:PRK05691 4266 FELGGHSLLATQIAS 4280
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
6-483 |
1.30e-80 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 274.47 E-value: 1.30e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 6 FEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTP 85
Cdd:PRK04813 8 IEEFAQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGHAYIPVDVSSP 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 86 AERVMVCLEDAQAVGIVTGHGLreGLDAIDCPIWDQPELMqkvdgpllrRGGAGPRHP-----------AYVIYTSGSTG 154
Cdd:PRK04813 88 AERIEMIIEVAKPSLIIATEEL--PLEILGIPVITLDELK---------DIFATGNPYdfdhavkgddnYYIIFTSGTTG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 155 KPKGVPINQGAICHFLRSENERLGVRADDRVY-QG-FSvaFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQG 232
Cdd:PRK04813 157 KPKGVQISHDNLVSFTNWMLEDFALPEGPQFLnQApYS--FDLSVMDLYPTLASGGTLVALPKDMTANFKQLFETLPQLP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 233 ITVLHAVPT------LLALFGAD-VPSLRLINLGGEICP----PALVERWatPTRRLFNSYGPTEATVSASLAE-----L 296
Cdd:PRK04813 235 INVWVSTPSfadmclLDPSFNEEhLPNLTHFLFCGEELPhktaKKLLERF--PSATIYNTYGPTEATVAVTSIEitdemL 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 297 RPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpnpwaEHAGEPRlYRTGDLARI 376
Cdd:PRK04813 313 DQYKRLPIGYAKPDSPLLIIDEEGTKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAFF-----TFDGQPA-YHTGDAGYL 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 377 DEqGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVL--KPVDGIDQLVAYIVPGGGQPVQIAQL----RAQ 450
Cdd:PRK04813 387 ED-GLLFYQGRIDFQIKLNGYRIELEEIEQNL-RQSSYVESAVVVpyNKDHKVQYLIAYVVPKEEDFEREFELtkaiKKE 464
|
490 500 510
....*....|....*....|....*....|....
gi 2772088563 451 LRETLPPYMVPSRFELVDTIPrLTS-GKIDRKAL 483
Cdd:PRK04813 465 LKERLMEYMIPRKFIYRDSLP-LTPnGKIDRKAL 497
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
142-479 |
2.26e-75 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 253.75 E-value: 2.26e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREitaDP 221
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKF---DP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLALARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEAT--VSA 291
Cdd:cd04433 78 EAALELIEREKVTILLGVPTLLARLlkapesaGYDLSSLRALVSGGAPLPPELLERFeEAPGIKLVNGYGLTETGgtVAT 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 292 SLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlpnpwaehagEPRLYRTG 371
Cdd:cd04433 158 GPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKGYWNNPEATAAVD----------EDGWYRTG 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 372 DLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPV-DGIDQLVAYIVPGGGQPVQIAQLRAQ 450
Cdd:cd04433 228 DLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDpEWGERVVAVVVLRPGADLDAEELRAH 307
|
330 340
....*....|....*....|....*....
gi 2772088563 451 LRETLPPYMVPSRFELVDTIPRLTSGKID 479
Cdd:cd04433 308 VRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
4-486 |
1.13e-70 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 248.10 E-value: 1.13e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVC-----GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWL 78
Cdd:COG0365 13 NCLDRHAEGRGDKVALIWegedgEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHS 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 79 P----FDADTPAERvmvcLEDAQAVGIVTGHGLREGLDAIDC-PIWDQ-----PELMQKVdgpLLRRGGAGPR------- 141
Cdd:COG0365 93 PvfpgFGAEALADR----IEDAEAKVLITADGGLRGGKVIDLkEKVDEaleelPSLEHVI---VVGRTGADVPmegdldw 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 -------------------HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENER-LGVRADDRVyqgFSVAfDMSfeeiW 201
Cdd:COG0365 166 dellaaasaefepeptdadDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYvLDLKPGDVF---WCTA-DIG----W 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 202 I---SYLV------GATLWIAP-REITADPDALPLALARQGITVLHAVPTLLALF---------GADVPSLRLINLGGEI 262
Cdd:COG0365 238 AtghSYIVygpllnGATVVLYEgRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALmkagdeplkKYDLSSLRLLGSAGEP 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 263 CPPALVERWatptRRLF-----NSYGPTEATvsASLAELRPGDPV---TIGQPLPNYGLLVVDAEMKLLPAGATGELCII 334
Cdd:COG0365 318 LNPEVWEWW----YEAVgvpivDGWGQTETG--GIFISNLPGLPVkpgSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 335 G--PGVAEGYLGRPELTAEKFlpnpWAEHAGeprLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQL 412
Cdd:COG0365 392 GpwPGMFRGYWNDPERYRETY----FGRFPG---WYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALV-SH 463
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 413 PN-GTAAVVLKPVDGIDQ-LVAYIVPGGGQPVQ---IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:COG0365 464 PAvAEAAVVGVPDEIRGQvVKAFVVLKPGVEPSdelAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKI 542
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
2-484 |
2.05e-70 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 244.01 E-value: 2.05e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:cd05936 1 LADLLEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDAidcpiwdqpelmqkvDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPI 161
Cdd:cd05936 81 PLYTPRELEHILNDSGAKALIVAVSFTDLLAA---------------GAPLGERVALTPEDVAVLQYTSGTTGVPKGAML 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 162 NQGAICHFLRSENERLGV--RADDR---------VYqGFSVAFDmsfeeiwISYLVGATLWIAPReitADPDALPLALAR 230
Cdd:cd05936 146 THRNLVANALQIKAWLEDllEGDDVvlaalplfhVF-GLTVALL-------LPLALGATIVLIPR---FRPIGVLKEIRK 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 231 QGITVLHAVPTLL-ALFGA------DVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEA----TVSASLAELRP 298
Cdd:cd05936 215 HRVTIFPGVPTMYiALLNApefkkrDFSSLRLCISGGAPLPVEVAERFEELTGvPIVEGYGLTETspvvAVNPLDGPRKP 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 299 GdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWaehageprlYRTGDLARIDE 378
Cdd:cd05936 295 G---SIGIPLPGTEVKIVDDDGEELPPGEVGELWVRGPQVMKGYWNRPEETAEAFV-DGW---------LRTGDIGYMDE 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 379 QGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLK---PVDGiDQLVAYIVPGGGQPVQIAQLRAQLRETL 455
Cdd:cd05936 362 DGYFFIVDRKKDMIIVGGFNVYPREVEEVLY-EHPAVAEAAVVGvpdPYSG-EAVKAFVVLKEGASLTEEEIIAFCREQL 439
|
490 500
....*....|....*....|....*....
gi 2772088563 456 PPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05936 440 AGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
1-485 |
1.13e-67 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 237.76 E-value: 1.13e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPF 80
Cdd:TIGR03098 1 LLHHLLEDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADTPAERVMVCLEDAQAVGIVTG--------HGLREGLDAIDCPIWDQPE-LMQKVDGP-------LLRRGGAGPRHP- 143
Cdd:TIGR03098 81 NPLLKAEQVAHILADCNVRLLVTSserldllhPALPGCHDLRTLIIVGDPAhASEGHPGEepaswpkLLALGDADPPHPv 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 -----AYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATL----WIAP 214
Cdd:TIGR03098 161 idsdmAAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVvlhdYLLP 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 215 REITAdpdalplALARQGITVLHAVPTL------LALFGADVPSLR-LINLGGEIcPPALVERWAT--PTRRLFNSYGPT 285
Cdd:TIGR03098 241 RDVLK-------ALEKHGITGLAAVPPLwaqlaqLDWPESAAPSLRyLTNSGGAM-PRATLSRLRSflPNARLFLMYGLT 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 286 EATVSASL-AELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPwaEHAGE 364
Cdd:TIGR03098 313 EAFRSTYLpPEEVDRRPDSIGKAIPNAEVLVLREDGSECAPGEEGELVHRGALVAMGYWNDPEKTAERFRPLP--PFPGE 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 365 PRLYRT----GDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAAL--SRQLPNGTAAVVLKPVDGiDQLVAYIVPGG 438
Cdd:TIGR03098 391 LHLPELavwsGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAyaTGLVAEAVAFGVPDPTLG-QAIVLVVTPPG 469
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 2772088563 439 GQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:TIGR03098 470 GEELDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
4-548 |
2.48e-62 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 234.19 E-value: 2.48e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSL---------SYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTG 74
Cdd:TIGR03443 240 DIFADNAEKHPDRTCVVETPSFLdpssktrsfTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAG 319
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 75 AAWLPFDADTPAERVMVCLEDAQAVGIVtghGLREG--LDAI--DCpIWDQPELMQKV-------DGPLlrRGGA----- 138
Cdd:TIGR03443 320 ATFSVIDPAYPPARQTIYLSVAKPRALI---VIEKAgtLDQLvrDY-IDKELELRTEIpalalqdDGSL--VGGSlegge 393
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 139 -------------------GPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEE 199
Cdd:TIGR03443 394 tdvlapyqalkdtptgvvvGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFPWMAKRFGLSENDKFTMLSGIAHDPIQRD 473
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 200 IWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLALFGAD----VPSLRLINLGGEIcppaLVERWAT-- 273
Cdd:TIGR03443 474 MFTPLFLGAQLLVPTADDIGTPGRLAEWMAKYGATVTHLTPAMGQLLSAQattpIPSLHHAFFVGDI----LTKRDCLrl 549
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 274 ----PTRRLFNSYGPTEATVSASLAEL--RPGDPVTI---------GQPLPNYGLLVVDA--EMKLLPAGATGELCIIGP 336
Cdd:TIGR03443 550 qtlaENVCIVNMYGTTETQRAVSYFEIpsRSSDSTFLknlkdvmpaGKGMKNVQLLVVNRndRTQTCGVGEVGEIYVRAG 629
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 337 GVAEGYLGRPELTAEKFLPN-----------------PWAEHAGEPR--LYRTGDLARIDEQGQIHCLGRADDQVKVRGF 397
Cdd:TIGR03443 630 GLAEGYLGLPELNAEKFVNNwfvdpshwidldkennkPEREFWLGPRdrLYRTGDLGRYLPDGNVECCGRADDQVKIRGF 709
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 398 RVELGEIEAALSRQlPNGTAAVVL--KPVDGIDQLVAYIVPGGGQP------------------VQ--------IAQLRA 449
Cdd:TIGR03443 710 RIELGEIDTHLSQH-PLVRENVTLvrRDKDEEPTLVSYIVPQDKSDeleefksevddeessdpvVKglikyrklIKDIRE 788
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 450 QLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALrAWP----MAALSATERSESDLPE-TEAETALFAALARFFPGLP-- 522
Cdd:TIGR03443 789 YLKKKLPSYAIPTVIVPLKKLPLNPNGKVDKPAL-PFPdtaqLAAVAKNRSASAADEEfTETEREIRDLWLELLPNRPat 867
|
650 660
....*....|....*....|....*.
gi 2772088563 523 IQRGGDFFdDLGGHSLLAARLVSALR 548
Cdd:TIGR03443 868 ISPDDSFF-DLGGHSILATRMIFELR 892
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
9-480 |
3.35e-61 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 216.32 E-value: 3.35e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 9 TAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAER 88
Cdd:cd17631 4 RARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPE 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 89 VMVCLEDAQAVGIVtghglregldaidcpiwdqpelmqkvdgpllrrggagpRHPAYVIYTSGSTGKPKGVPINQGAICH 168
Cdd:cd17631 84 VAYILADSGAKVLF--------------------------------------DDLALLMYTSGTTGRPKGAMLTHRNLLW 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 169 FLRSENERLGVRADDRvyqgFSVAFDMSFEE----IWISYL-VGATLWIAPReitADPDALPLALARQGITVLHAVPTLL 243
Cdd:cd17631 126 NAVNALAALDLGPDDV----LLVVAPLFHIGglgvFTLPTLlRGGTVVILRK---FDPETVLDLIERHRVTSFFLVPTMI 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 -------ALFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLaeLRPGD----PVTIGQPLPNYG 312
Cdd:cd17631 199 qallqhpRFATTDLSSLRAVIYGGAPMPERLLRALQARGVKFVQGYGMTETSPGVTF--LSPEDhrrkLGSAGRPVFFVE 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 313 LLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQV 392
Cdd:cd17631 277 VRIVDPDGREVPPGEVGEIVVRGPHVMAGYWNRPEATAAAF-RDGW---------FHTGDLGRLDEDGYLYIVDRKKDMI 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 393 KVRGFRVELGEIEAALSrQLPN-GTAAVVLKP-VDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTI 470
Cdd:cd17631 347 ISGGENVYPAEVEDVLY-EHPAvAEVAVIGVPdEKWGEAVVAVVVPRPGAELDEDELIAHCRERLARYKIPKSVEFVDAL 425
|
490
....*....|
gi 2772088563 471 PRLTSGKIDR 480
Cdd:cd17631 426 PRNATGKILK 435
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
2-485 |
6.96e-61 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 218.13 E-value: 6.96e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWL---PRGIDLLasqLAIAKTGAAWL 78
Cdd:PRK06187 8 IGRILRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDwnsHEYLEAY---FAVPKIGAVLH 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 79 PFDADTPAERVMVCLEDAQAVGIVTGH-------GLREGLDAIDCPIWDQPELMQKVDGPLLR----RGGA--------- 138
Cdd:PRK06187 85 PINIRLKPEEIAYILNDAEDRVVLVDSefvpllaAILPQLPTVRTVIVEGDGPAAPLAPEVGEyeelLAAAsdtfdfpdi 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 139 GPRHPAYVIYTSGSTGKPKGVpinqgAICH---FLRSE--NERLGVRADDRvyqgFSVAFDM--SFEeIWISYL---VGA 208
Cdd:PRK06187 165 DENDAAAMLYTSGTTGHPKGV-----VLSHrnlFLHSLavCAWLKLSRDDV----YLVIVPMfhVHA-WGLPYLalmAGA 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 209 TLwIAPREItaDPDALPLALARQGITVLHAVPTLLAL-------FGADVPSLRLINLGGEICPPALVERW-ATPTRRLFN 280
Cdd:PRK06187 235 KQ-VIPRRF--DPENLLDLIETERVTFFFAVPTIWQMllkapraYFVDFSSLRLVIYGGAALPPALLREFkEKFGIDLVQ 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 281 SYGPTEATVSASLAELRPGDP------VTIGQPLPNYGLLVVDAEMKLLPA--GATGELCIIGPGVAEGYLGRPELTAEK 352
Cdd:PRK06187 312 GYGMTETSPVVSVLPPEDQLPgqwtkrRSAGRPLPGVEARIVDDDGDELPPdgGEVGEIIVRGPWLMQGYWNRPEATAET 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 353 FlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvdgiDQL-- 430
Cdd:PRK06187 392 I-DGGW---------LHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVP----DEKwg 457
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 2772088563 431 ---VAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK06187 458 erpVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLRE 515
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
2-485 |
7.81e-59 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 212.07 E-value: 7.81e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK07656 7 LPELLARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLN 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 -ADTPAE----------RVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVD--GPLLRRGGAGPRHPAY--- 145
Cdd:PRK07656 87 tRYTADEaayilargdaKALFVLGLFLGVDYSATTRLPALEHVVICETEEDDPHTEKMKtfTDFLAAGDPAERAPEVdpd 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 ----VIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQ--------GFSVAfdmsfeeiWISYLV-GATLWI 212
Cdd:PRK07656 167 dvadILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYLAanpffhvfGYKAG--------VNAPLMrGATILP 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 213 APreiTADPDALPLALARQGITVLHAVPT----LLALFGA---DVPSLRLINLGGEICPPALVERWatptRRLFN----- 280
Cdd:PRK07656 239 LP---VFDPDEVFRLIETERITVLPGPPTmynsLLQHPDRsaeDLSSLRLAVTGAASMPVALLERF----ESELGvdivl 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 281 -SYGPTEATVSASLAelRPGDPV-----TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFL 354
Cdd:PRK07656 312 tGYGLSEASGVTTFN--RLDDDRktvagTIGTAIAGVENKIVNELGEEVPVGEVGELLVRGPNVMKGYYDDPEATAAAID 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 355 PNPWaehageprLYrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLV-AY 433
Cdd:PRK07656 390 ADGW--------LH-TGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGkAY 460
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 434 IVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK07656 461 VVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
14-483 |
6.22e-54 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 197.74 E-value: 6.22e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALV-------CGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPA 86
Cdd:cd17647 2 PERTCVVetpslnsSKTRSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ERVMVCLEDAQAVGIVtghglregldaidcpiwdqpelmqkvdgpLLRRGGA--GPRHPAYVIYTSGSTGKPKGVPINQG 164
Cdd:cd17647 82 ARQNIYLGVAKPRGLI-----------------------------VIRAAGVvvGPDSNPTLSFTSGSEGIPKGVLGRHF 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 165 AICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTLLA 244
Cdd:cd17647 133 SLAYYFPWMAKRFNLSENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTQDDIGTPGRLAEWMAKYGATVTHLTPAMGQ 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 LFGAD----VPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAEL--RPGDP---------VTIGQP 307
Cdd:cd17647 213 LLTAQattpFPKLHHAFFVGDILTKRDCLRLQTlaENVRIVNMYGTTETQRAVSYFEVpsRSSDPtflknlkdvMPAGRG 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 308 LPNYGLLVVDA--EMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPN-----------------PWAEHAGEPR-- 366
Cdd:cd17647 293 MLNVQLLVVNRndRTQICGIGEVGEIYVRAGGLAEGYRGLPELNKEKFVNNwfvepdhwnyldkdnnePWRQFWLGPRdr 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 367 LYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVL--KPVDGIDQLVAYIVPGGGQPVQ- 443
Cdd:cd17647 373 LYRTGDLGRYLPNGDCECCGRADDQVKIRGFRIELGEIDTHIS-QHPLVRENITLvrRDKDEEPTLVSYIVPRFDKPDDe 451
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2772088563 444 --------------------------IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd17647 452 sfaqedvpkevstdpivkgligyrklIKDIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVDKPKL 517
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
25-483 |
1.23e-52 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 191.92 E-value: 1.23e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVT- 103
Cdd:cd17654 16 TVSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAAYAPIDPASPEQRSLTVMKKCHVSYLLQn 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 104 GHGLREGLDAIDcpiwdQPELMQK-VDGPLlrrggagprhpAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRAD 182
Cdd:cd17654 96 KELDNAPLSFTP-----EHRHFNIrTDECL-----------AYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSE 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 183 DrVYQGFSVA-FDMSFEEIWISYLVGATLWIAPREITADPDALPLALA-RQGITVLHAVPTLLALFGADVP--------- 251
Cdd:cd17654 160 D-ILFLTSPLtFDPSVVEIFLSLSSGATLLIVPTSVKVLPSKLADILFkRHRITVLQATPTLFRRFGSQSIkstvlsats 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 SLRLINLGGEICPPALV-ERWAT--PTRRLFNSYGPTEATVSASLAELRPGD-PVTIGQPLPNYGLLVVDAEmkllPAGA 327
Cdd:cd17654 239 SLRVLALGGEPFPSLVIlSSWRGkgNRTRIFNIYGITEVSCWALAYKVPEEDsPVQLGSPLLGTVIEVRDQN----GSEG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 328 TGEL---CIIGPGVAEGYLGRPELTaekflpnpwaehageprLYRTGDLARIdEQGQIHCLGRADDQVKVRGFRVELGEI 404
Cdd:cd17654 315 TGQVflgGLNRVCILDDEVTVPKGT-----------------MRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLI 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 405 EAALSRQLPNGTAAVVLKpvdGIDQLVAYIVpggGQPVQiAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd17654 377 QQVIESCLGVESCAVTLS---DQQRLIAFIV---GESSS-SRIHKELQLTlLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
46-484 |
1.53e-52 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 191.88 E-value: 1.53e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 46 GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAW----LPFDADTPAE--RVMVCLEDAQAVGIVTGHGLREGLDAIDCPIw 119
Cdd:cd05922 14 GGVRGERVVLILPNRFTYIELSFAVAYAGGRLglvfVPLNPTLKESvlRYLVADAGGRIVLADAGAADRLRDALPASPD- 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 120 dqPELMQKVDGPLLRRGGAgPRHP------AYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAF 193
Cdd:cd05922 93 --PGTVLDADGIRAARASA-PAHEvshedlALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADDRALTVLPLSY 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 194 DMSFEEIWISYLVGATLWIAPREITadPDALPLALARQGITVLHAVPTLLALFG------ADVPSLRLINLGGEICPPAL 267
Cdd:cd05922 170 DYGLSVLNTHLLRGATLVLTNDGVL--DDAFWEDLREHGATGLAGVPSTYAMLTrlgfdpAKLPSLRYLTQAGGRLPQET 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 268 VERWAT--PTRRLFNSYGPTEATVSASL--AELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYL 343
Cdd:cd05922 248 IARLREllPGAQVYVMYGQTEATRRMTYlpPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHRGPNVMKGYW 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 344 GRPeltaekflPNPWAEHAGEPRLYrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKP 423
Cdd:cd05922 328 NDP--------PYRRKEGRGGGVLH-TGDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLP 398
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 424 VDGIDQLVAYIVpgGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05922 399 DPLGEKLALFVT--APDKIDPKDVLRSLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
15-485 |
2.96e-51 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 187.88 E-value: 2.96e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 15 DQTALVCGALSLSYRELDERASLMAHHLIKQG-VRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGkDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAELEYVI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVtghglregldaidcpiwdqpelmqkvdgpllrrggagprHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:cd05941 81 TDSEPSLVL---------------------------------------DPALILYTSGTTGRPKGVVLTHANLAANVRAL 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQ--------GFSVAFDMSFeeiwisyLVGATLwiaprEITADPDALPLALAR--QGITVLHAVPT-- 241
Cdd:cd05941 122 VDAWRWTEDDVLLHvlplhhvhGLVNALLCPL-------FAGASV-----EFLPKFDPKEVAISRlmPSITVFMGVPTiy 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 242 --LLALFGADVP-----------SLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEATVSASL---AELRPGdpvTI 304
Cdd:cd05941 190 trLLQYYEAHFTdpqfaraaaaeRLRLMVSGSAALPVPTLEEWEAITgHTLLERYGMTEIGMALSNpldGERRPG---TV 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 305 GQPLPNYGLLVVDAE-MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIH 383
Cdd:cd05941 267 GMPLPGVQARIVDEEtGEPLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW---------FKTGDLGVVDEDGYYW 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 384 CLGR-ADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVP-GGGQPVQIAQLRAQLRETLPPYMV 460
Cdd:cd05941 338 ILGRsSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGErVVAVVVLrAGAAALSLEELKEWAKQRLAPYKR 417
|
490 500
....*....|....*....|....*
gi 2772088563 461 PSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05941 418 PRRLILVDELPRNAMGKVNKKELRK 442
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
27-484 |
3.24e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 187.11 E-value: 3.24e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGhg 106
Cdd:cd05934 5 TYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVVD-- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 107 lregldaidcpiwdqpelmqkvdgpllrrggagprhPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDrVY 186
Cdd:cd05934 83 ------------------------------------PASILYTSGTTGPPKGVVITHANLTFAGYYSARRFGLGEDD-VY 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 187 QGFSVAFDMS--FEEIWISYLVGATLWIAPReitADPDALPLALARQGITVLHAVPTLLALFGADVPS-------LRLIn 257
Cdd:cd05934 126 LTVLPLFHINaqAVSVLAALSVGATLVLLPR---FSASRFWSDVRRYGATVTNYLGAMLSYLLAQPPSpddrahrLRAA- 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 258 LGGEICP---PALVERWATPtrrLFNSYGPTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCI- 333
Cdd:cd05934 202 YGAPNPPelhEEFEERFGVR---LLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIr 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 334 --IGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQ 411
Cdd:cd05934 279 glRGWGFFKGYYNMPEATAEAM-RNGW---------FHTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRH 348
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 412 LPNGTAAVVLKPVD-GIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05934 349 PAVREAAVVAVPDEvGEDEVKAVVVLRPGETLDPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
16-484 |
5.36e-51 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 186.90 E-value: 5.36e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 16 QTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawLPFDADT---PAERVMVc 92
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGA--IAVVINPllhPDDYAYI- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 LEDAQAVGIVTGHglreglDAIdcpiwdqpelmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQGAICHFLRS 172
Cdd:cd05919 78 ARDCEARLVVTSA------DDI-----------------------------AYLLYSSGTTGPPKGVMHAHRDPLLFADA 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 173 -ENERLGVRADDRVYQGFSVAFDMSF-EEIWISYLVGATLWIAPREITadPDALPLALARQGITVLHAVPTLLA------ 244
Cdd:cd05919 123 mAREALGLTPGDRVFSSAKMFFGYGLgNSLWFPLAVGASAVLNPGWPT--AERVLATLARFRPTVLYGVPTFYAnlldsc 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 -LFGADVPSLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEAT---VSASLAELRPGdpvTIGQPLPNYGLLVVDAE 319
Cdd:cd05919 201 aGSPDALRSLRLCVSAGEALPRGLGERWMEHFgGPILDGIGATEVGhifLSNRPGAWRLG---STGRPVPGYEIRLVDEE 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 320 MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRV 399
Cdd:cd05919 278 GHTIPPGEEGDLLVRGPSAAVGYWNNPEKSRATFN-GGW---------YRTGDKFCRDADGWYTHAGRADDMLKVGGQWV 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 400 ELGEIEAALSRQLPNGTAAVVLKP-VDGIDQLVAYIVPGGGQPVQIA---QLRAQLRETLPPYMVPSRFELVDTIPRLTS 475
Cdd:cd05919 348 SPVEVESLIIQHPAVAEAAVVAVPeSTGLSRLTAFVVLKSPAAPQESlarDIHRHLLERLSAHKVPRRIAFVDELPRTAT 427
|
....*....
gi 2772088563 476 GKIDRKALR 484
Cdd:cd05919 428 GKLQRFKLR 436
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
4-484 |
9.76e-49 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 182.18 E-value: 9.76e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd05959 8 LVDLNLNEGRGDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TPAERVMVCLEDAQA-VGIVTGHglregldaidcpIWDQPELMQKVDGPLLRR----GGAGPRH---------------- 142
Cdd:cd05959 88 LTPDDYAYYLEDSRArVVVVSGE------------LAPVLAAALTKSEHTLVVlivsGGAGPEAgalllaelvaaeaeql 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 ---------PAYVIYTSGSTGKPKGVPINQGAICHFLRSENER-LGVRADDRVYQ----------GFSVAFDMSfeeiwi 202
Cdd:cd05959 156 kpaathaddPAFWLYSSGSTGRPKGVVHLHADIYWTAELYARNvLGIREDDVCFSaaklffayglGNSLTFPLS------ 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 203 sylVGATLWIAPREITadPDALPLALARQGITVLHAVPTLLA-LFGADVP------SLRLINLGGEICPPALVERWatpt 275
Cdd:cd05959 230 ---VGATTVLMPERPT--PAAVFKRIRRYRPTVFFGVPTLYAaMLAAPNLpsrdlsSLRLCVSAGEALPAEVGERW---- 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 276 RRLF-----NSYGPTEAT---VSASLAELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPE 347
Cdd:cd05959 301 KARFgldilDGIGSTEMLhifLSNRPGRVRYG---TTGKPVPGYEVELRDEDGGDVADGEPGELYVRGPSSATMYWNNRD 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 348 LTAEKFLpNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVV-LKPVDG 426
Cdd:cd05959 378 KTRDTFQ-GEW---------TRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVgVEDEDG 447
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 427 IDQLVAYIVPGGGQPVQIA---QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05959 448 LTKPKAFVVLRPGYEDSEAleeELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
2-486 |
2.56e-47 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 178.42 E-value: 2.56e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawLPFD 81
Cdd:COG1021 27 LGDLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGA--IPVF 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 AdTPAERVM----VClEDAQAVGIVT-----GHGLREGLDAI--DCP------IWDQPE--------LMQKVDGPLLRrg 136
Cdd:COG1021 105 A-LPAHRRAeishFA-EQSEAVAYIIpdrhrGFDYRALARELqaEVPslrhvlVVGDAGeftsldalLAAPADLSEPR-- 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 137 gAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVA--FDMSFEEIWISYLVGATLWIAP 214
Cdd:COG1021 181 -PDPDDVAFFQLSGGTTGLPKLIPRTHDDYLYSVRASAEICGLDADTVYLAALPAAhnFPLSSPGVLGVLYAGGTVVLAP 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 215 reiTADPD-ALPLaLARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERwATPTR--RLFNSYGP 284
Cdd:COG1021 260 ---DPSPDtAFPL-IERERVTVTALVPPLALLWldaaersRYDLSSLRVLQVGGAKLSPELARR-VRPALgcTLQQVFGM 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 285 TEATVSASlaelRPGDPV-----TIGQPLPNYG-LLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPW 358
Cdd:COG1021 335 AEGLVNYT----RLDDPEeviltTQGRPISPDDeVRIVDEDGNPVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGF 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 359 aehageprlYRTGDLARIDEQGQIHCLGRADDQVkVRGfrvelGE------IEAALSRqLPN-GTAAVVLKPvdgiDQLV 431
Cdd:COG1021 411 ---------YRTGDLVRRTPDGYLVVEGRAKDQI-NRG-----GEkiaaeeVENLLLA-HPAvHDAAVVAMP----DEYL 470
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 432 -----AYIVPGGgQPVQIAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:COG1021 471 gerscAFVVPRG-EPLTLAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKIDKKALRAA 530
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
2-484 |
2.74e-47 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 178.71 E-value: 2.74e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGAL------SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGA 75
Cdd:PRK13295 26 INDDLDACVASCPDKTAVTAVRLgtgaprRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 76 AWLP------------------------------FDADTPAERVMVCLEDAQAVGIVTGHGlREGLDAIDC-PIWDQpel 124
Cdd:PRK13295 106 VLNPlmpifrerelsfmlkhaeskvlvvpktfrgFDHAAMARRLRPELPALRHVVVVGGDG-ADSFEALLItPAWEQ--- 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 125 mQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEE-IWIS 203
Cdd:PRK13295 182 -EPDAPAILARLRPGPDDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTGFMYgLMMP 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 204 YLVGAT-----LWiapreitaDPDALPLALARQGITVLHAVPTLL-------ALFGADVPSLRLINLGGEICPPALVER- 270
Cdd:PRK13295 261 VMLGATavlqdIW--------DPARAAELIRTEGVTFTMASTPFLtdltravKESGRPVSSLRTFLCAGAPIPGALVERa 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 271 WATPTRRLFNSYGPTEatvSASLAELRPGDP-----VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGR 345
Cdd:PRK13295 333 RAALGAKIVSAWGMTE---NGAVTLTKLDDPderasTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQVRGCSNFGGYLKR 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 346 PELTAEKFlpNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVD 425
Cdd:PRK13295 410 PQLNGTDA--DGW---------FDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDE 478
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 426 GI-DQLVAYIVPGGGQPVQIAQLRAQLRE--TLPPYMvPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK13295 479 RLgERACAFVVPRPGQSLDFEEMVEFLKAqkVAKQYI-PERLVVRDALPRTPSGKIQKFRLR 539
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
25-485 |
3.18e-47 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 174.84 E-value: 3.18e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPAERVMVcLEDAQAvgivt 103
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRlTPNELAFQ-LKDSDV----- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 104 ghglreGLDAIdcpiwdqpelmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADD 183
Cdd:cd05912 75 ------KLDDI-----------------------------ATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGLTEDD 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 184 R------VYQ--GFSVAFDmsfeeiwiSYLVGATLWIAPReitADPDALPLALARQGITVLHAVPT----LLALFGADVP 251
Cdd:cd05912 120 NwlcalpLFHisGLSILMR--------SVIYGMTVYLVDK---FDAEQVLHLINSGKVTIISVVPTmlqrLLEILGEGYP 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 -SLRLINLGGEICPPALVERWATPTRRLFNSYGPTE-----ATVSASLAELRPGdpvTIGQPLPNYGLLVVDAEMkllPA 325
Cdd:cd05912 189 nNLRCILLGGGPAPKPLLEQCKEKGIPVYQSYGMTEtcsqiVTLSPEDALNKIG---SAGKPLFPVELKIEDDGQ---PP 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 326 GATGELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIE 405
Cdd:cd05912 263 YEVGEILLKGPNVTKGYLNRPDATEESF-ENGW---------FKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIE 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 406 AALsRQLPN-GTAAVVLKPVDGIDQL-VAYIVpgGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd05912 333 EVL-LSHPAiKEAGVVGIPDDKWGQVpVAFVV--SERPISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHEL 409
|
..
gi 2772088563 484 RA 485
Cdd:cd05912 410 KQ 411
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
2-484 |
5.91e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 177.05 E-value: 5.91e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK08316 13 IGDILRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVN 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDA------IDCPIWDQPELMQKVDGPLL---RRGGAGPRH---------- 142
Cdd:PRK08316 93 FMLTGEELAYILDHSGARAFLVDPALAPTAEAalallpVDTLILSLVLGGREAPGGWLdfaDWAEAGSVAepdveladdd 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR------VYQgfSVAFDMsFEEIWIsyLVGATLWIAPRe 216
Cdd:PRK08316 173 LAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIplhalpLYH--CAQLDV-FLGPYL--YVGATNVILDA- 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 217 itADPDALPLALARQGITVLHAVPT----LL--ALFG-ADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTE- 286
Cdd:PRK08316 247 --PDPELILRTIEAERITSFFAPPTvwisLLrhPDFDtRDLSSLRKGYYGASIMPVEVLKELRErlPGLRFYNCYGQTEi 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 287 ---ATVsaslaeLRPGD----PVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWa 359
Cdd:PRK08316 325 aplATV------LGPEEhlrrPGSAGRPVLNVETRVVDDDGNDVAPGEVGEIVHRSPQLMLGYWDDPEKTAEAFR-GGW- 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 360 ehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDG-IDQLVAYIVPGG 438
Cdd:PRK08316 397 --------FHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKwIEAVTAVVVPKA 468
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2772088563 439 GQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK08316 469 GATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELR 514
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
14-484 |
2.64e-45 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 171.73 E-value: 2.64e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVC--GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMV 91
Cdd:cd05926 1 PDAPALVVpgSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 92 CLEDAQAVGIVTGHG----------------LREGLDAIDCPIWDQPE---LMQKVDGPLLRRGGAGPRHPAYVIYTSGS 152
Cdd:cd05926 81 YLADLGSKLVLTPKGelgpasraasklglaiLELALDVGVLIRAPSAEslsNLLADKKNAKSEGVPLPDDLALILHTSGT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICHFLRSENERLGVRADDRVY--------QGFSVAFdmsfeeiwISYLV-GATLWIAPREITAD--P 221
Cdd:cd05926 161 TGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLvvmplfhvHGLVASL--------LSTLAaGGSVVLPPRFSASTfwP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DalplaLARQGITVLHAVPTLL--------ALFGADVPSLRLINLGGEICPP----ALVERWATPtrrLFNSYGPTEATV 289
Cdd:cd05926 233 D-----VRDYNATWYTAVPTIHqillnrpePNPESPPPKLRFIRSCSASLPPavleALEATFGAP---VLEAYGMTEAAH 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 290 SASLAELRPGD--PVTIGQPLpNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprl 367
Cdd:cd05926 305 QMTSNPLPPGPrkPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGW--------- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 368 YRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLK-PVDGIDQLV-AYIVPGGGQPVQIA 445
Cdd:cd05926 375 FRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVL-LSHPAVLEAVAFGvPDEKYGEEVaAAVVLREGASVTEE 453
|
490 500 510
....*....|....*....|....*....|....*....
gi 2772088563 446 QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05926 454 ELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVA 492
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
25-485 |
4.69e-44 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 166.40 E-value: 4.69e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPfdadtpaerVMVCLEDAQAVGIvtg 104
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNP---------ILPFFREHELAFI--- 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 hglregLDAIDCPIWDQPELMQKvdgpllRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR 184
Cdd:cd05903 69 ------LRRAKAKVFVVPERFRQ------FDPAAMPDAVALLLFTSGTTGEPKGVMHSHNTLSASIRQYAERLGLGPGDV 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 185 V--------YQGFSVAFDMSFeeiwisyLVGAT-----LWiapreitaDPDALPLALARQGITVLHAVPTLLALF----- 246
Cdd:cd05903 137 FlvaspmahQTGFVYGFTLPL-------LLGAPvvlqdIW--------DPDKALALMREHGVTFMMGATPFLTDLlnave 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 --GADVPSLRLINLGGEICPPALVER-WATPTRRLFNSYGPTEatVSASLAELRPGDP----VTIGQPLPNYGLLVVDAE 319
Cdd:cd05903 202 eaGEPLSRLRTFVCGGATVPRSLARRaAELLGAKVCSAYGSTE--CPGAVTSITPAPEdrrlYTDGRPLPGVEIKVVDDT 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 320 MKLLPAGATGELCIIGPGVAEGYLGRPELTAEkFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRV 399
Cdd:cd05903 280 GATLAPGVEGELLSRGPSVFLGYLDRPDLTAD-AAPEGW---------FRTGDLARLDEDGYLRITGRSKDIIIRGGENI 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 400 ELGEIEAALSRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGGQPVQIAQLRAQL-RETLPPYMVPSRFELVDTIPRLTSGK 477
Cdd:cd05903 350 PVLEVEDLLLGHPGVIEAAVVALPDERLGERAcAVVVTKSGALLTFDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGK 429
|
....*...
gi 2772088563 478 IDRKALRA 485
Cdd:cd05903 430 VQKFRLRE 437
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
2-483 |
1.29e-43 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 166.35 E-value: 1.29e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawLPFD 81
Cdd:cd05920 17 LGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGA--VPVL 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 AdTPAERVM----VClEDAQAVGIVtGHGLREGLDAidcpiwdqpelmqkvdGPLLRRGGAGPRHPAYVIYTSGSTGKPK 157
Cdd:cd05920 95 A-LPSHRRSelsaFC-AHAEAVAYI-VPDRHAGFDH----------------RALARELAESIPEVALFLLSGGTTGTPK 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 158 GVPINQGAICHFLRSENERLGVRADDRVYQGFSVA--FDMSFEEIWISYLVGATLWIAPreiTADPD-ALPLaLARQGIT 234
Cdd:cd05920 156 LIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAhnFPLACPGVLGTLLAGGRVVLAP---DPSPDaAFPL-IEREGVT 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 235 VLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEATVSASlaelRPGDP----- 301
Cdd:cd05920 232 VTALVPALVSLWldaaasrRADLSSLRLLQVGGARLSPALARRVpPVLGCTLQQVFGMAEGLLNYT----RLDDPdevii 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 302 VTIGQPL-PNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQG 380
Cdd:cd05920 308 HTQGRPMsPDDEIRVVDEEGNPVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGF---------YRTGDLVRRTPDG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 381 QIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvdgiDQL-----VAYIVPgGGQPVQIAQLRAQLRET- 454
Cdd:cd05920 379 YLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMP----DELlgersCAFVVL-RDPPPSAAQLRRFLRERg 453
|
490 500
....*....|....*....|....*....
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd05920 454 LAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
25-478 |
3.64e-43 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 165.08 E-value: 3.64e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPAE----------RVMVC- 92
Cdd:cd05911 10 ELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIyTADElahqlkiskpKVIFTd 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 -------LEDAQAVG-----IVTGHGLREGLDAIDcpIWDQPELMQKVDGPLLRrgGAGPRHPAYVIYTSGSTGKPKGVP 160
Cdd:cd05911 90 pdglekvKEAAKELGpkdkiIVLDDKPDGVLSIED--LLSPTLGEEDEDLPPPL--KDGKDDTAAILYSSGTTGLPKGVC 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 161 I-NQGAICHFLRSEN-ERLGVRADDRVYqGFSVAFDMS-FEEIWISYLVGATLWIAPReitADPDALPLALARQGITVLH 237
Cdd:cd05911 166 LsHRNLIANLSQVQTfLYGNDGSNDVIL-GFLPLYHIYgLFTTLASLLNGATVIIMPK---FDSELFLDLIEKYKITFLY 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 238 AVPTLLALFG-------ADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVSASLAELRPGDPVTIGQPL 308
Cdd:cd05911 242 LVPPIAAALAksplldkYDLSSLRVILSGGAPLSKELQELLAKrfPNATIKQGYGMTETGGILTVNPDGDDKPGSVGRLL 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 309 PNYGLLVVDAE-MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGR 387
Cdd:cd05911 322 PNVEAKIVDDDgKDSLGPNEPGEICVRGPQVMKGYYNNPEATKETFDEDGW---------LHTGDIGYFDEDGYLYIVDR 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 388 ADDQVKVRGFRVELGEIEAALsRQLPN-GTAAVV-LKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLREtlppyMVPSRFE 465
Cdd:cd05911 393 KKELIKYKGFQVAPAELEAVL-LEHPGvADAAVIgIPDEVSGELPRAYVVRKPGEKLTEKEVKDYVAK-----KVASYKQ 466
|
490
....*....|....*....
gi 2772088563 466 L------VDTIPRLTSGKI 478
Cdd:cd05911 467 LrggvvfVDEIPKSASGKI 485
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
26-485 |
6.89e-43 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 162.89 E-value: 6.89e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTgh 105
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 glreglDAIDcpiwdqpelmqkvdgpllrrggagprhPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRV 185
Cdd:cd05972 79 ------DAED---------------------------PALIYFTSGTTGLPKGVLHTHSYPLGHIPTAAYWLGLRPDDIH 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 186 YQ----GFSVAFDMSFEEIWisyLVGATLwIAPREITADPDALPLALARQGITVLHAVPTLLALF------GADVPSLRL 255
Cdd:cd05972 126 WNiadpGWAKGAWSSFFGPW---LLGATV-FVYEGPRFDAERILELLERYGVTSFCGPPTAYRMLikqdlsSYKFSHLRL 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 256 INLGGEICPPALVERW-ATPTRRLFNSYGPTE--ATVSASL-AELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGEL 331
Cdd:cd05972 202 VVSAGEPLNPEVIEWWrAATGLPIRDGYGQTEtgLTVGNFPdMPVKPG---SMGRPTPGYDVAIIDDDGRELPPGEEGDI 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 332 CI-IGP-GVAEGYLGRPELTAEKFlpnpwaeHAGeprLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALS 409
Cdd:cd05972 279 AIkLPPpGLFLGYVGDPEKTEASI-------RGD---YYLTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALL 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 410 RQLPNGTAAVVLKPVDGIDQLV-AYIV--PGGGQPVQIAQ-LRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05972 349 EHPAVAEAAVVGSPDPVRGEVVkAFVVltSGYEPSEELAEeLQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
26-486 |
1.28e-42 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 162.29 E-value: 1.28e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP-FDADTPaERVMVCLEDAQAVGIVTg 104
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPlFSAFGP-EAIRDRLENSEAKVLIT- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 hglregldaidcpiwdQPELMQKVDgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR 184
Cdd:cd05969 79 ----------------TEELYERTD----------PEDPTLLHYTSGTTGTPKGVLHVHDAMIFYYFTGKYVLDLHPDDI 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 185 VYQGFSVAF-DMSFEEIWISYLVGATLWIAPREItaDPDALPLALARQGITVLHAVPT---LLALFGA------DVPSLR 254
Cdd:cd05969 133 YWCTADPGWvTGTVYGIWAPWLNGVTNVVYEGRF--DAESWYGIIERVKVTVWYTAPTairMLMKEGDelarkyDLSSLR 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 255 LINLGGEICPPALVeRWATPT--RRLFNSYGPTEaTVSASLAELrPGDPV---TIGQPLPNYGLLVVDAEMKLLPAGATG 329
Cdd:cd05969 211 FIHSVGEPLNPEAI-RWGMEVfgVPIHDTWWQTE-TGSIMIANY-PCMPIkpgSMGKPLPGVKAAVVDENGNELPPGTKG 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 330 ELCIIG--PGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAA 407
Cdd:cd05969 288 ILALKPgwPSMFRGIWNDEERYKNSF-IDGW---------YLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESA 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 408 LSRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGG-QPVQiaQLRAQL----RETLPPYMVPSRFELVDTIPRLTSGKIDRK 481
Cdd:cd05969 358 LMEHPAVAEAGVIGKPDPLRGEIIkAFISLKEGfEPSD--ELKEEIinfvRQKLGAHVAPREIEFVDNLPKTRSGKIMRR 435
|
....*
gi 2772088563 482 ALRAW 486
Cdd:cd05969 436 VLKAK 440
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
16-484 |
1.03e-41 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 159.57 E-value: 1.03e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 16 QTALVCGALSLSYRELDERASLMAHHLI-KQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAwlpfdadtpaervmvcle 94
Cdd:cd05958 1 RTCLRSPEREWTYRDLLALANRIANVLVgELGIVPGNRVLLRGSNSPELVACWFGIQKAGAI------------------ 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 95 daqAVGIVTGhgLREGldAIDCPIWDQPELMQKVDGPLLRRGGAGprhpaYVIYTSGSTGKPKGVPinqgaicHFLRSEN 174
Cdd:cd05958 63 ---AVATMPL--LRPK--ELAYILDKARITVALCAHALTASDDIC-----ILAFTSGTTGAPKATM-------HFHRDPL 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 175 ER--------LGVRADDRVYQGFSVAFDMSFEEIWI-SYLVGATLWIAPReitADPDALPLALARQGITVLHAVPT---- 241
Cdd:cd05958 124 ASadryavnvLRLREDDRFVGSPPLAFTFGLGGVLLfPFGVGASGVLLEE---ATPDLLLSAIARYKPTVLFTAPTayra 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 242 ---LLALFGADVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEAT---VSASLAELRPGdpvTIGQPLPNYGLL 314
Cdd:cd05958 201 mlaHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATGiPIIDGIGSTEMFhifISARPGDARPG---ATGKPVPGYEAK 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 315 VVDAEMKLLPAGATGELCIIGPgvaEGYLGRPELTAEKFLPNPWaehageprLYrTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:cd05958 278 VVDDEGNPVPDGTIGRLAVRGP---TGCRYLADKRQRTYVQGGW--------NI-TGDTYSRDPDGYFRHQGRSDDMIVS 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGGQ---PVQIAQLRAQLRETLPPYMVPSRFELVDTI 470
Cdd:cd05958 346 GGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVkAFVVLRPGVipgPVLARELQDHAKAHIAPYKYPRAIEFVTEL 425
|
490
....*....|....
gi 2772088563 471 PRLTSGKIDRKALR 484
Cdd:cd05958 426 PRTATGKLQRFALR 439
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
25-486 |
8.35e-41 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 158.26 E-value: 8.35e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHhLIKQGVRPGQIVGLWLPRGIDLLASQLAIA--------------------------------- 71
Cdd:cd05909 7 SLTYRKLLTGAIALAR-KLAKMTKEGENVGVMLPPSAGGALANFALAlsgkvpvmlnytaglrelraciklagiktvlts 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 72 ----KTGAAWLPFDADTPAErvMVCLEDAQAvGIVTGHGLREGLDAIDCPIWdqpelmqkvdgpLLRRGGAGPRHP---A 144
Cdd:cd05909 86 kqfiEKLKLHHLFDVEYDAR--IVYLEDLRA-KISKADKCKAFLAGKFPPKW------------LLRIFGVAPVQPddpA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 145 YVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQ--------GFSVAfdmsfeeIWISYLVGATLWIAPRE 216
Cdd:cd05909 151 VILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGalpffhsfGLTGC-------LWLPLLSGIKVVFHPNP 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 217 ItaDPDALPLALARQGITVLHAVPTLLALF-----GADVPSLRLINLGGEICPPALVERWA-TPTRRLFNSYGPTEAT-- 288
Cdd:cd05909 224 L--DYKKIPELIYDKKATILLGTPTFLRGYaraahPEDFSSLRLVVAGAEKLKDTLRQEFQeKFGIRILEGYGTTECSpv 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 VSASLAEL--RPGdpvTIGQPLPNYGLLVVDAEMKL-LPAGATGELCIIGPGVAEGYLGRPELTAEKFlpnpwaeHAGep 365
Cdd:cd05909 302 ISVNTPQSpnKEG---TVGRPLPGMEVKIVSVETHEeVPIGEGGLLLVRGPNVMLGYLNEPELTSFAF-------GDG-- 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 366 rLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPN-GTAAVVLKPvDGI--DQLVAYIVPGGGQPv 442
Cdd:cd05909 370 -WYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEILPEdNEVAVVSVP-DGRkgEKIVLLTTTTDTDP- 446
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 2772088563 443 qiAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:cd05909 447 --SSLNDILKNAgISNLAKPSYIHQVEEIPLLGTGKPDYVTLKAL 489
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
2-493 |
1.29e-40 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 158.75 E-value: 1.29e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGA------ 75
Cdd:PRK06164 12 LASLLDAHARARPDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGAtviavn 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 76 -----------------AWL------------------PFDADTPAERVMVCLEDAQAVgivTGHGLREGLDAIDCPiwD 120
Cdd:PRK06164 92 tryrshevahilgrgraRWLvvwpgfkgidfaailaavPPDALPPLRAIAVVDDAADAT---PAPAPGARVQLFALP--D 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 121 QPELmqkvdgPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEI 200
Cdd:PRK06164 167 PAPP------AAAGERAADPDAGALLFTTSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 201 WISYLVGATLWIAPreiTADPDALPLALARQGITvlHAVPT------LLALFG--ADVPSLRLINLG------GEIcpPA 266
Cdd:PRK06164 241 LGALAGGAPLVCEP---VFDAARTARALRRHRVT--HTFGNdemlrrILDTAGerADFPSARLFGFAsfapalGEL--AA 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 267 LVERWATPTRRLfnsYGPTEatVSASLAELRPGDPVTI-----GQPL-PNYGLLVVDAEM-KLLPAGATGELCIIGPGVA 339
Cdd:PRK06164 314 LARARGVPLTGL---YGSSE--VQALVALQPATDPVSVrieggGRPAsPEARVRARDPQDgALLPDGESGEIEIRAPSLM 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 340 EGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV 419
Cdd:PRK06164 389 RGYLDNPDATARALTDDGY---------FRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQV 459
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 420 VLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSG---KIDRKALRAWPMAALSA 493
Cdd:PRK06164 460 VGATRDGKTVPVAFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQARLAA 536
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
27-485 |
1.46e-40 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 156.44 E-value: 1.46e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGhg 106
Cdd:cd05971 8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTD-- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 107 lregldaidcpiwdqpelmqkvdgpllrrggaGPRHPAYVIYTSGSTGKPKGVpinqgaichflrsenerlgVRADdRVY 186
Cdd:cd05971 86 --------------------------------GSDDPALIIYTSGTTGPPKGA-------------------LHAH-RVL 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 187 QGFSVAFDMSFEEI--------------WISYLVGATL--W------IAPREITADPDALPLALARQGITVLHAVPTLLA 244
Cdd:cd05971 114 LGHLPGVQFPFNLFprdgdlywtpadwaWIGGLLDVLLpsLyfgvpvLAHRMTKFDPKAALDLMSRYGVTTAFLPPTALK 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 LFGADVP-------SLRLINLGGEicPPALVE-RWAtptRRLFNS-----YGPTEAT-VSASLAELRPGDPVTIGQPLPN 310
Cdd:cd05971 194 MMRQQGEqlkhaqvKLRAIATGGE--SLGEELlGWA---REQFGVevnefYGQTECNlVIGNCSALFPIKPGSMGKPIPG 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 311 YGLLVVDAEMKLLPAGATGELCIIGPGVAE--GYLGRPELTAEKFLpNPWaehageprlYRTGDLARIDEQGQIHCLGRA 388
Cdd:cd05971 269 HRVAIVDDNGTPLPPGEVGEIAVELPDPVAflGYWNNPSATEKKMA-GDW---------LLTGDLGRKDSDGYFWYVGRD 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 389 DDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvDGI--DQLVAYIV--PGGGQPVQIA-QLRAQLRETLPPYMVPSR 463
Cdd:cd05971 339 DDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIP-DPIrgEIVKAFVVlnPGETPSDALArEIQELVKTRLAAHEYPRE 417
|
490 500
....*....|....*....|..
gi 2772088563 464 FELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05971 418 IEFVNELPRTATGKIRRRELRA 439
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
14-486 |
1.26e-39 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 154.35 E-value: 1.26e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPAERVMVc 92
Cdd:PRK03640 16 PDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRlSREELLWQ- 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 LEDAQAVGIVTGHGLrEGLDAIDCPIwDQPELMQKVDGPL-------LRRggagprhPAYVIYTSGSTGKPKGVPINQGA 165
Cdd:PRK03640 95 LDDAEVKCLITDDDF-EAKLIPGISV-KFAELMNGPKEEAeiqeefdLDE-------VATIMYTSGTTGKPKGVIQTYGN 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 166 icHFL--RSENERLGVRADDRVY--------QGFSVAFDmsfeeiwiSYLVGATLWIAPReitADPDALPLALARQGITV 235
Cdd:PRK03640 166 --HWWsaVGSALNLGLTEDDCWLaavpifhiSGLSILMR--------SVIYGMRVVLVEK---FDAEKINKLLQTGGVTI 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 236 LHAVPTLLALFGADVP------SLRLINLGGEICPPALVE---RWATPtrrLFNSYGPTEaTVSaSLAELRPGDPVT--- 303
Cdd:PRK03640 233 ISVVSTMLQRLLERLGegtypsSFRCMLLGGGPAPKPLLEqckEKGIP---VYQSYGMTE-TAS-QIVTLSPEDALTklg 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 304 -IGQPLPNYGLLVVDaEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWaehageprlYRTGDLARIDEQGQI 382
Cdd:PRK03640 308 sAGKPLFPCELKIEK-DGVVVPPFEEGEIVVKGPNVTKGYLNREDATRETFQ-DGW---------FKTGDIGYLDEEGFL 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 383 HCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVpgGGQPVQIAQLRAQLRETLPPYMVP 461
Cdd:PRK03640 377 YVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVpVAFVV--KSGEVTEEELRHFCEEKLAKYKVP 454
|
490 500
....*....|....*....|....*
gi 2772088563 462 SRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK03640 455 KRFYFVEELPRNASGKLLRHELKQL 479
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
10-485 |
2.62e-39 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 154.70 E-value: 2.62e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALV------CGALSLSYRELDERASLMAHHLIKQGvRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD 83
Cdd:cd05931 3 AAARPDRPAYTflddegGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 TP---AERVMVCLEDAQAVGIVTGHGLREGLDAI--DCPIWDQP-----ELMQKVDGPLLRRGGAGPRHPAYVIYTSGST 153
Cdd:cd05931 82 TPgrhAERLAAILADAGPRVVLTTAAALAAVRAFaaSRPAAGTPrllvvDLLPDTSAADWPPPSPDPDDIAYLQYTSGST 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 154 GKPKGVPINQGAICHFLRSENERLGVRADDR--------------------VYQGFSVAF--DMSF---EEIWISYL--V 206
Cdd:cd05931 162 GTPKGVVVTHRNLLANVRQIRRAYGLDPGDVvvswlplyhdmgligglltpLYSGGPSVLmsPAAFlrrPLRWLRLIsrY 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 207 GATlwiapreITADPD-ALPLALARqgitvlhAVPTLLAlfGADVPSLRLINLGGEICPPALVERWATPTRR-------L 278
Cdd:cd05931 242 RAT-------ISAAPNfAYDLCVRR-------VRDEDLE--GLDLSSWRVALNGAEPVRPATLRRFAEAFAPfgfrpeaF 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 279 FNSYGPTEATVSASLAelRPGDPVTI----------------------------GQPLPNYGLLVVDAE-MKLLPAGATG 329
Cdd:cd05931 306 RPSYGLAEATLFVSGG--PPGTGPVVlrvdrdalagravavaaddpaarelvscGRPLPDQEVRIVDPEtGRELPDGEVG 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 330 ELCIIGPGVAEGYLGRPELTAEKFLPnpwAEHAGEPRLYRTGDLARIDEqGQIHCLGRADDQVKVRGFRVELGEIEAALS 409
Cdd:cd05931 384 EIWVRGPSVASGYWGRPEATAETFGA---LAATDEGGWLRTGDLGFLHD-GELYITGRLKDLIIVRGRNHYPQDIEATAE 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 410 RQLPN---GTAAVVLKPVDGIDQLVAYI-VPGGGQPVQIAQLRAQLRETlppymVPSRFELV---------DTIPRLTSG 476
Cdd:cd05931 460 EAHPAlrpGCVAAFSVPDDGEERLVVVAeVERGADPADLAAIAAAIRAA-----VAREHGVApadvvlvrpGSIPRTSSG 534
|
....*....
gi 2772088563 477 KIDRKALRA 485
Cdd:cd05931 535 KIQRRACRA 543
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
2-484 |
1.86e-38 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 152.62 E-value: 1.86e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCG--ALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP 79
Cdd:PRK12583 20 IGDAFDATVARFPDREALVVRhqALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVN 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 FDADTPAERVMVCLEDAQAVGIVTGHGLR-----EGLDAIDCPIWD-QPELMQKVDGPLLRR----GGAGPRH------- 142
Cdd:PRK12583 100 INPAYRASELEYALGQSGVRWVICADAFKtsdyhAMLQELLPGLAEgQPGALACERLPELRGvvslAPAPPPGflawhel 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 -----------------------PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR--VYQGFSVAFDMSF 197
Cdd:PRK12583 180 qargetvsrealaerqasldrddPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRlcVPVPLYHCFGMVL 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 198 EEIwISYLVGATLwIAPREiTADPDALPLALARQGITVLHAVPTLLAL------FGA-DVPSLRLINLGGEICPPALVER 270
Cdd:PRK12583 260 ANL-GCMTVGACL-VYPNE-AFDPLATLQAVEEERCTALYGVPTMFIAeldhpqRGNfDLSSLRTGIMAGAPCPIEVMRR 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 271 WATPTR--RLFNSYGPTEAT-VSASLAelrPGDPV-----TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGY 342
Cdd:PRK12583 337 VMDEMHmaEVQIAYGMTETSpVSLQTT---AADDLerrveTVGRTQPHLEVKVVDPDGATVPRGEIGELCTRGYSVMKGY 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 343 LGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLK 422
Cdd:PRK12583 414 WNNPEATAESIDEDGW---------MHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGV 484
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 423 PVDGI-DQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK12583 485 PDEKYgEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMR 547
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
2-484 |
2.08e-38 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 152.21 E-value: 2.08e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALV--CGAlSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP 79
Cdd:PRK06087 25 LADYWQQTARAMPDKIAVVdnHGA-SYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVP 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 FDADTPAERVMVCLEDAQAVGIVTG------------HGLREGLDAID--------CPIWDQPELMQKVDG--PLLRRGG 137
Cdd:PRK06087 104 LLPSWREAELVWVLNKCQAKMFFAPtlfkqtrpvdliLPLQNQLPQLQqivgvdklAPATSSLSLSQIIADyePLTTAIT 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 138 AGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFeeiwiSYLVGATLWIAPREI 217
Cdd:PRK06087 184 THGDELAAVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGF-----LHGVTAPFLIGARSV 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 TAD---PDALPLALARQGIT-VLHAVPTLLALF------GADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEa 287
Cdd:PRK06087 259 LLDiftPDACLALLEQQRCTcMLGATPFIYDLLnllekqPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVYGSTE- 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 288 tvSASLAELRPGDPV-----TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaeha 362
Cdd:PRK06087 338 --SSPHAVVNLDDPLsrfmhTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRGPNVFMGYLDEPELTARALDEEGW---- 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 363 geprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGI-DQLVAYIVPGGG-Q 440
Cdd:PRK06087 412 -----YYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLgERSCAYVVLKAPhH 486
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 2772088563 441 PVQIAQLRAQL-RETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK06087 487 SLTLEEVVAFFsRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLR 531
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
1-484 |
3.18e-38 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 150.80 E-value: 3.18e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTAL---VCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAW 77
Cdd:PRK07514 1 MNNNLFDALRAAFADRDAPfieTPDGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 78 LPFD-ADTPAE----------RVMVC----LEDAQAVGIVTGHGLREGLDAidcpiwdqpelmqKVDGPLLRRG-GAGPR 141
Cdd:PRK07514 81 LPLNtAYTLAEldyfigdaepALVVCdpanFAWLSKIAAAAGAPHVETLDA-------------DGTGSLLEAAaAAPDD 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HP---------AYVIYTSGSTGKPKGVPINQGAichfLRSENERL----GVRADDRVYQ--------GFSVAfdmsfeeI 200
Cdd:PRK07514 148 FEtvprgaddlAAILYTSGTTGRSKGAMLSHGN----LLSNALTLvdywRFTPDDVLIHalpifhthGLFVA-------T 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 201 WISYLVGATLWIAPReitADPDALPLALARQgiTVLHAVPT----LLA---LFGADVPSLRLINLGGeicPPALVE---R 270
Cdd:PRK07514 217 NVALLAGASMIFLPK---FDPDAVLALMPRA--TVMMGVPTfytrLLQeprLTREAAAHMRLFISGS---APLLAEthrE 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 271 WATPT-RRLFNSYGPTEATVSAS---LAELRPGdpvTIGQPLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGR 345
Cdd:PRK07514 289 FQERTgHAILERYGMTETNMNTSnpyDGERRAG---TVGFPLPGVSLRVTDPETgAELPPGEIGMIEVKGPNVFKGYWRM 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 346 PELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPnGTA--AVVLKP 423
Cdd:PRK07514 366 PEKTAEEFRADGF---------FITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEID-ELP-GVVesAVIGVP 434
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 424 -VDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK07514 435 hPDFGEGVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
14-485 |
4.53e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 150.91 E-value: 4.53e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVG-LWLPRG---IDLLASQLA-------------------I 70
Cdd:PRK06188 26 PDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVAlLSLNRPevlMAIGAAQLAglrrtalhplgslddhayvL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 71 AKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGHGlrEGLDAIDcpIWDQPELMQkvDGPLlrRGGAGPRHPAYVIYTS 150
Cdd:PRK06188 106 EDAGISTLIVDPAPFVERALALLARVPSLKHVLTLG--PVPDGVD--LLAAAAKFG--PAPL--VAAALPPDIAGLAYTG 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 151 GSTGKPKGVPINQGAICHFLRSENERLGVRADDRvyqgFSVAFDMSFEE---IWISYLVGATLWIAPReitADPDALPLA 227
Cdd:PRK06188 178 GTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPR----FLMCTPLSHAGgafFLPTLLRGGTVIVLAK---FDPAEVLRA 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGITVLHAVPTLL-------ALFGADVPSLRLINLGGE-ICPPALVERWATPTRRLFNSYGPTEATVSASL---AEL 296
Cdd:PRK06188 251 IEEQRITATFLVPTMIyalldhpDLRTRDLSSLETVYYGASpMSPVRLAEAIERFGPIFAQYYGQTEAPMVITYlrkRDH 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 297 RPGDPVTI---GQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDL 373
Cdd:PRK06188 331 DPDDPKRLtscGRPTPGLRVALLDEDGREVAQGEVGEICVRGPLVMDGYWNRPEETAEAF-RDGW---------LHTGDV 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 374 ARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGGQPVQIAQLRAQLR 452
Cdd:PRK06188 401 AREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVtAVVVLRPGAAVDAAELQAHVK 480
|
490 500 510
....*....|....*....|....*....|...
gi 2772088563 453 ETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK06188 481 ERKGSVHAPKQVDFVDSLPLTALGKPDKKALRA 513
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
4-484 |
9.52e-38 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 149.95 E-value: 9.52e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALV-CGALS----LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWL 78
Cdd:cd05970 21 DVVDAMAKEYPDKLALVwCDDAGeeriFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAI 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 79 PFDADTPAERVMVCLEDAQ--AVGIVTGHGLREGLDAI--DCPIwdqPELMQKVDGPLL------------------RRG 136
Cdd:cd05970 101 PATHQLTAKDIVYRIESADikMIVAIAEDNIPEEIEKAapECPS---KPKLVWVGDPVPegwidfrkliknaspdfeRPT 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 137 G---AGPRHPAYVIYTSGSTGKPKGVP---------INQGAICHFLRSENERLGVrADdrvyQGFSVAFDMSFEEIWISy 204
Cdd:cd05970 178 AnsyPCGEDILLVYFSSGTTGMPKMVEhdftyplghIVTAKYWQNVREGGLHLTV-AD----TGWGKAVWGKIYGQWIA- 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 lvGATLWIAPREiTADPDALPLALARQGITVLHAVPTLLA------LFGADVPSLRLINLGGEICPPALVERWATPTR-R 277
Cdd:cd05970 252 --GAAVFVYDYD-KFDPKALLEKLSKYGVTTFCAPPTIYRfliredLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGiK 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 278 LFNSYGPTEATVS-ASLAELRPgDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCI-----IGPGVAEGYLGRPELTAE 351
Cdd:cd05970 329 LMEGFGQTETTLTiATFPWMEP-KPGSMGKPAPGYEIDLIDREGRSCEAGEEGEIVIrtskgKPVGLFGGYYKDAEKTAE 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 352 KFlpnpwaeHAGeprLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV--VLKPVDGidQ 429
Cdd:cd05970 408 VW-------HDG---YYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPAVLECAVtgVPDPIRG--Q 475
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 430 LV-AYIVPGGG-QPVQ--IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05970 476 VVkATIVLAKGyEPSEelKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
7-483 |
4.36e-37 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 147.31 E-value: 4.36e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 7 EATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-T 84
Cdd:PRK06839 9 EKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRlT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAE----------RVMVCLEDAQAVGIvtghgLREGLDAIDCPIW--DQPELMQKVDGPLLRRGGAGPrhpaYVI-YTSG 151
Cdd:PRK06839 89 ENElifqlkdsgtTVLFVEKTFQNMAL-----SMQKVSYVQRVISitSLKEIEDRKIDNFVEKNESAS----FIIcYTSG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 152 STGKPKGVPINQGAIchFLRSENERLGV--RADDRvyqgfSVAFDMSFEEIWISYLVGATLWIAPREITA---DPDALPL 226
Cdd:PRK06839 160 TTGKPKGAVLTQENM--FWNALNNTFAIdlTMHDR-----SIVLLPLFHIGGIGLFAFPTLFAGGVIIVPrkfEPTKALS 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 227 ALARQGITVLHAVPTL-LALFGA------DVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEA--TVSASLAELR 297
Cdd:PRK06839 233 MIEKHKVTVVMGVPTIhQALINCskfettNLQSVRWFYNGGAPCPEELMREFIDRGFLFGQGFGMTETspTVFMLSEEDA 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKfLPNPWaehageprlYRTGDLARID 377
Cdd:PRK06839 313 RRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRGPNVMKEYWNRPDATEET-IQDGW---------LCTGDLARVD 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 378 EQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLRETLP 456
Cdd:PRK06839 383 EDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIpIAFIVKKSSSVLIEKDVIEHCRLFLA 462
|
490 500
....*....|....*....|....*..
gi 2772088563 457 PYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:PRK06839 463 KYKIPKEIVFLKELPKNATGKIQKAQL 489
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
2-479 |
8.95e-37 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 146.95 E-value: 8.95e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAwlPFD 81
Cdd:PRK07798 5 IADLFEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAV--PVN 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 AD---TPAERVMVcLEDAQAVGIVTGHGLREGLDAI--DCP-------IWDQPELMQKVDG----PLLRRGGAGPRHPA- 144
Cdd:PRK07798 83 VNyryVEDELRYL-LDDSDAVALVYEREFAPRVAEVlpRLPklrtlvvVEDGSGNDLLPGAvdyeDALAAGSPERDFGEr 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 145 -----YVIYTSGSTGKPKGVPINQGAICH-FLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLV-GATLWIA---- 213
Cdd:PRK07798 162 spddlYLLYTGGTTGMPKGVMWRQEDIFRvLLGGRDFATGEPIEDEEELAKRAAAGPGMRRFPAPPLMhGAGQWAAfaal 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 214 --------PREITADPDALPLALARQGITVLHAV------PTLLALF---GADVPSLRLINLGGEICPPALVERW--ATP 274
Cdd:PRK07798 242 fsgqtvvlLPDVRFDADEVWRTIEREKVNVITIVgdamarPLLDALEargPYDLSSLFAIASGGALFSPSVKEALleLLP 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 275 TRRLFNSYGPTEA-----TVSASLAELRPGDPVTIGQplpnyGLLVVDAEMKLLPAGaTGELCII--GPGVAEGYLGRPE 347
Cdd:PRK07798 322 NVVLTDSIGSSETgfggsGTVAKGAVHTGGPRFTIGP-----RTVVLDEDGNPVEPG-SGEIGWIarRGHIPLGYYKDPE 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 348 LTAEKFLpnpwaEHAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGI 427
Cdd:PRK07798 396 KTAETFP-----TIDGV-RYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERW 469
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 428 DQLVAYIV-PGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKID 479
Cdd:PRK07798 470 GQEVVAVVqLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKAD 522
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
25-469 |
1.16e-36 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 145.05 E-value: 1.16e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTG 104
Cdd:cd05907 5 PITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 HglregldaidcpiwdqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR 184
Cdd:cd05907 85 D----------------------------------PDDLATIIYTSGTTGRPKGVMLSHRNILSNALALAERLPATEGDR 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 185 vyqgfSVAF-DMS--FEEIWISYLV---GATLWIAPreitaDPDALPLALARQGITVLHAVPTLL-----ALFGADVP-- 251
Cdd:cd05907 131 -----HLSFlPLAhvFERRAGLYVPllaGARIYFAS-----SAETLLDDLSEVRPTVFLAVPRVWekvyaAIKVKAVPgl 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 -----------SLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLAELRPGDPVTIGQPLPnygllvvDAEM 320
Cdd:cd05907 201 krklfdlavggRLRFAASGGAPLPAELLHFFRALGIPVYEGYGLTETSAVVTLNPPGDNRIGTVGKPLP-------GVEV 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 321 KLlpaGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADD-QVKVRGFRV 399
Cdd:cd05907 274 RI---ADDGEILVRGPNVMLGYYKNPEATAEALDADGW---------LHTGDLGEIDEDGFLHITGRKKDlIITSGGKNI 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 400 ELGEIEAALSRQLPNGTAAVVLkpvDGIDQLVAYIVP---------------------GGGQPVQIAQLRAQLR---ETL 455
Cdd:cd05907 342 SPEPIENALKASPLISQAVVIG---DGRPFLVALIVPdpealeawaeehgiaytdvaeLAANPAVRAEIEAAVEaanARL 418
|
490
....*....|....
gi 2772088563 456 PPYMVPSRFELVDT 469
Cdd:cd05907 419 SRYEQIKKFLLLPE 432
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
27-484 |
4.04e-36 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 144.70 E-value: 4.04e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQGVRPGQIVG-----------LWL-------------PRgidLLASQLA--IAKTGAAWLPF 80
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVAtlawnthrhleLYYavpgmgavlhtinPR---LFPEQIAyiINHAEDRVVFV 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADT-----------PAERVMVCLEDAQAVGIVTGHGLREGLDAIDC--PIWDQPElmqkVDGpllrrggagpRHPAYVI 147
Cdd:cd12119 104 DRDFlplleaiaprlPTVEHVVVMTDDAAMPEPAGVGVLAYEELLAAesPEYDWPD----FDE----------NTAAAIC 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 148 YTSGSTGKPKGVPINQGAIchFLRSenerLGVRADDRVYQG-----------FSV-AFDMSFeeiwISYLVGATLWIAPR 215
Cdd:cd12119 170 YTSGTTGNPKGVVYSHRSL--VLHA----MAALLTDGLGLSesdvvlpvvpmFHVnAWGLPY----AAAMVGAKLVLPGP 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 216 EitADPDALPLALARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTE-- 286
Cdd:cd12119 240 Y--LDPASLAELIEREGVTFAAGVPTVWQGLldhleanGRDLSSLRRVVIGGSAVPRSLIEAFEERGVRVIHAWGMTEts 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 287 --ATVSASLAELRPGDP-------VTIGQPLPNYGLLVVDAEMKLLPA--GATGELCIIGPGVAEGYLGRPElTAEKFLP 355
Cdd:cd12119 318 plGTVARPPSEHSNLSEdeqlalrAKQGRPVPGVELRIVDDDGRELPWdgKAVGELQVRGPWVTKSYYKNDE-ESEALTE 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 356 NPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYI 434
Cdd:cd12119 397 DGW---------LRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERpLAVV 467
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 2772088563 435 VPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd12119 468 VLKEGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALR 517
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
2-485 |
5.59e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 145.18 E-value: 5.59e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK06178 35 LTEYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVS 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVT-------------GHGLREGL---------DAIDCPIwdqPELMQ----KVDG----- 130
Cdd:PRK06178 115 PLFREHELSYELNDAGAEVLLAldqlapvveqvraETSLRHVIvtsladvlpAEPTLPL---PDSLRaprlAAAGaidll 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 131 PLLRRGGAGPRHP-------AYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFsvafdmsFEEIWIS 203
Cdd:PRK06178 192 PALRACTAPVPLPppaldalAALNYTGGTTGMPKGCEHTQRDMVYTAAAAYAVAVVGGEDSVFLSF-------LPEFWIA 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 204 ---------YLVGATLWIAPReitADPDALPLALARQGIT----VLHAVPTLLALFGA---DVPSLRLINLGGEI--CPP 265
Cdd:PRK06178 265 genfgllfpLFSGATLVLLAR---WDAVAFMAAVERYRVTrtvmLVDNAVELMDHPRFaeyDLSSLRQVRVVSFVkkLNP 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 266 ALVERWATPTRRLF--NSYGPTEATVSASLA--------ELRpGDPVTIGQPLPNYGLLVVDAEM-KLLPAGATGELCII 334
Cdd:PRK06178 342 DYRQRWRALTGSVLaeAAWGMTETHTCDTFTagfqdddfDLL-SQPVFVGLPVPGTEFKICDFETgELLPLGAEGEIVVR 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 335 GPGVAEGYLGRPELTAEKFLpNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPN 414
Cdd:PRK06178 421 TPSLLKGYWNKPEATAEALR-DGW---------LHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAV 490
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 415 GTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSrFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK06178 491 LGSAVVGRPDPDKGQVpVAFVQLKPGADLTAAALQAWCRENMAVYKVPE-IRIVDALPMTATGKVRKQDLQA 561
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
25-483 |
1.28e-35 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 141.46 E-value: 1.28e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTG 104
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 hglrEGLDAIdcpiwdqpelmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVpinqgAICHFLRSENERLGVRADDR 184
Cdd:cd05935 81 ----SELDDL-----------------------------ALIPYTSGTTGLPKGC-----MHTHFSAAANALQSAVWTGL 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 185 VYQGFSVAFDMSFE------EIWISYLVGATLWIAPReitADPDALPLALARQGITVLHAVPTLLALFGA-------DVP 251
Cdd:cd05935 123 TPSDVILACLPLFHvtgfvgSLNTAVYVGGTYVLMAR---WDRETALELIEKYKVTFWTNIPTMLVDLLAtpefktrDLS 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 SLRLINLGGEICPPALVERWATPTRRLFNS-YGPTEATVSASLAElrPGDP--VTIGQPLPNYGLLVVDAE-MKLLPAGA 327
Cdd:cd05935 200 SLKVLTGGGAPMPPAVAEKLLKLTGLRFVEgYGLTETMSQTHTNP--PLRPklQCLGIP*FGVDARVIDIEtGRELPPNE 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 328 TGELCIIGPGVAEGYLGRPELTAEKFLpnpwaEHAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAA 407
Cdd:cd05935 278 VGEIVVRGPQIFKGYWNRPEETEESFI-----EIKGR-RFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAK 351
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 408 LSRQLPNGTAAVVLKPVDGIDQLV-AYIV--PGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd05935 352 LYKHPAI*EVCVISVPDERVGEEVkAFIVlrPEYRGKVTEEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
2-485 |
1.76e-35 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 143.60 E-value: 1.76e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGA------ 75
Cdd:PRK05605 34 LVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAvvvehn 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 76 ---------------------AW---------LPfdADTPAERVMvcledaqAVGIVTGHGLREGLdAIDCPIwdqPELM 125
Cdd:PRK05605 114 plytahelehpfedhgarvaiVWdkvaptverLR--RTTPLETIV-------SVNMIAAMPLLQRL-ALRLPI---PALR 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 126 QKVD---GP---------LLRRGGAG-----------PRHPAYVIYTSGSTGKPKGVPINQGAIChflrsENERLG---- 178
Cdd:PRK05605 181 KARAaltGPapgtvpwetLVDAAIGGdgsdvshprptPDDVALILYTSGTTGKPKGAQLTHRNLF-----ANAAQGkawv 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 179 ---VRADDRVY------QGFSVAFDMSFeeiwiSYLVGATLWIAPReitADPDALPLALARQGITVLHAVPTLLALF--- 246
Cdd:PRK05605 256 pglGDGPERVLaalpmfHAYGLTLCLTL-----AVSIGGELVLLPA---PDIDLILDAMKKHPPTWLPGVPPLYEKIaea 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 ----GADVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTE----ATVSASLAELRPGdpvTIGQPLPNYGLLVVD 317
Cdd:PRK05605 328 aeerGVDLSGVRNAFSGAMALPVSTVELWEKLTGgLLVEGYGLTEtspiIVGNPMSDDRRPG---YVGVPFPDTEVRIVD 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 318 AE--MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNpWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVR 395
Cdd:PRK05605 405 PEdpDETMPDGEEGELLVRGPQVFKGYWNRPEETAKSFLDG-W---------FRTGDVVVMEEDGFIRIVDRIKELIITG 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 396 GFRVELGEIEAALsRQLPNGT-AAVVLKP-VDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRL 473
Cdd:PRK05605 475 GFNVYPAEVEEVL-REHPGVEdAAVVGLPrEDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPRD 553
|
570
....*....|..
gi 2772088563 474 TSGKIDRKALRA 485
Cdd:PRK05605 554 QLGKVRRREVRE 565
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
10-483 |
1.87e-35 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 142.37 E-value: 1.87e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGAL--SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD-ADTPA 86
Cdd:cd05904 15 ASAHPSRPALIDAATgrALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANpLSTPA 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ErVMVCLEDAQAVGIVTGHGLREGLDAIDCPI--WDQPE--------LMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKP 156
Cdd:cd05904 95 E-IAKQVKDSGAKLAFTTAELAEKLASLALPVvlLDSAEfdslsfsdLLFEADEAEPPVVVIKQDDVAALLYSSGTTGRS 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 157 KGVPINQG----AICHFLRSENERlgvRADDRVYQGFSVAFD-MSFEEIWISYL-VGATLWIAPReitADPDALPLALAR 230
Cdd:cd05904 174 KGVMLTHRnliaMVAQFVAGEGSN---SDSEDVFLCVLPMFHiYGLSSFALGLLrLGATVVVMPR---FDLEELLAAIER 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 231 QGITVLHAVP-TLLAL------FGADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEAT-VSAS--LAELRP 298
Cdd:cd05904 248 YKVTHLPVVPpIVLALvkspivDKYDLSSLRQIMSGAAPLGKELIEAFRAkfPNVDLGQGYGMTESTgVVAMcfAPEKDR 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 299 GDPVTIGQPLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARID 377
Cdd:cd05904 328 AKYGSVGRLVPNVEAKIVDPETgESLPPNQTGELWIRGPSIMKGYLNNPEATAATIDKEGW---------LHTGDLCYID 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 378 EQGQIHCLGRADDQVKVRGFRVELGEIEAAL-SRqlPNGT-AAVVLKPVDGIDQL-VAYIV--PGGG-QPVQIAQLRAql 451
Cdd:cd05904 399 EDGYLFIVDRLKELIKYKGFQVAPAELEALLlSH--PEILdAAVIPYPDEEAGEVpMAFVVrkPGSSlTEDEIMDFVA-- 474
|
490 500 510
....*....|....*....|....*....|..
gi 2772088563 452 rETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd05904 475 -KQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
14-478 |
4.74e-35 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 142.72 E-value: 4.74e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALV------CGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAE 87
Cdd:cd17634 67 GDRTAIIyegddtSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPE 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 88 RVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGP----LLRRGG-------------------AGPRH-- 142
Cdd:cd17634 147 AVAGRIIDSSSRLLITADGGVRAGRSVPLKKNVDDALNPNVTSVehviVLKRTGsdidwqegrdlwwrdliakASPEHqp 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 -------PAYVIYTSGSTGKPKGV-PINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEeiWISY---LVGATLW 211
Cdd:cd17634 227 eamnaedPLFILYTSGTTGKPKGVlHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHS--YLLYgplACGATTL 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 212 I---APreITADPDALPLALARQGITVLHAVPTLL---------ALFGADVPSLRLINLGGEICPPAlVERWAT-----P 274
Cdd:cd17634 305 LyegVP--NWPTPARMWQVVDKHGVNILYTAPTAIralmaagddAIEGTDRSSLRILGSVGEPINPE-AYEWYWkkigkE 381
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 275 TRRLFNSYGPTEatVSASLAELRPG-DPVTIG---QPLPNYGLLVVDAEMKLLPAGATGELCIIG--PGVAEGYLGRPEl 348
Cdd:cd17634 382 KCPVVDTWWQTE--TGGFMITPLPGaIELKAGsatRPVFGVQPAVVDNEGHPQPGGTEGNLVITDpwPGQTRTLFGDHE- 458
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 349 taeKFLPNPWAEHAGeprLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVV--LKPVDG 426
Cdd:cd17634 459 ---RFEQTYFSTFKG---MYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVgiPHAIKG 532
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 427 iDQLVAYIVPGGGQ---PVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:cd17634 533 -QAPYAYVVLNHGVepsPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKI 586
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
1-486 |
6.95e-35 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 140.30 E-value: 6.95e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLwLPRGIDLLasQLAIAKTGAAW--L 78
Cdd:PRK07638 2 GITKEYKKHASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKESKNKTIAIL-LENRIEFL--QLFAGAAMAGWtcV 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 79 PFDADTPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLLRRG-GAGPRH-PAYVIYTSGSTGKP 156
Cdd:PRK07638 79 PLDIKWKQDELKERLAISNADMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYApIENVQNaPFYMGFTSGSTGKP 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 157 KGVPINQGAICHFLRSENERLGVRADDRVyqgfSVA---FDMSFEEIWISYL-VGATLWIAPREItadPDALPLALARQG 232
Cdd:PRK07638 159 KAFLRAQQSWLHSFDCNVHDFHMKREDSV----LIAgtlVHSLFLYGAISTLyVGQTVHLMRKFI---PNQVLDKLETEN 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 233 ITVLHAVPTLL-ALFGAD-VP--SLRLINLGGEICPPA---LVERWatPTRRLFNSYGPTEAT-VSASLAELRPGDPVTI 304
Cdd:PRK07638 232 ISVMYTVPTMLeSLYKENrVIenKMKIISSGAKWEAEAkekIKNIF--PYAKLYEFYGASELSfVTALVDEESERRPNSV 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 305 GQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELtAEKFLPNPWAEhageprlyrTGDLARIDEQGQIHC 384
Cdd:PRK07638 310 GRPFHNVQVRICNEAGEEVQKGEIGTVYVKSPQFFMGYIIGGVL-ARELNADGWMT---------VRDVGYEDEEGFIYI 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 385 LGRADDQVKVRGFRVELGEIEAalsrqlpngtaavVLKPVDGIDQLVAYIVPG---GGQPVQIA-------QLRAQLRET 454
Cdd:PRK07638 380 VGREKNMILFGGINIFPEEIES-------------VLHEHPAVDEIVVIGVPDsywGEKPVAIIkgsatkqQLKSFCLQR 446
|
490 500 510
....*....|....*....|....*....|..
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK07638 447 LSSFKIPKEWHFVDEIPYTNSGKIARMEAKSW 478
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
2-485 |
7.14e-35 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 141.33 E-value: 7.14e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK07470 9 LAHFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTN 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 -ADTPAE----------RVMVCLED----AQAVgivtghglREGLDAIDCPIW-DQPELMQKVDGPLLRRGGAGPR---- 141
Cdd:PRK07470 89 fRQTPDEvaylaeasgaRAMICHADfpehAAAV--------RAASPDLTHVVAiGGARAGLDYEALVARHLGARVAnaav 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 ---HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERL--GVRADDR------------VYQGFSVAfdmsfeeiwisy 204
Cdd:PRK07470 161 dhdDPCWFFFTSGTTGRPKAAVLTHGQMAFVITNHLADLmpGTTEQDAslvvaplshgagIHQLCQVA------------ 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 lVGATLWIAPREiTADPDALPLALARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVER-WATPTR 276
Cdd:PRK07470 229 -RGAATVLLPSE-RFDPAEVWALVERHRVTNLFTVPTILKMLvehpavdRYDHSSLRYVIYAGAPMYRADQKRaLAKLGK 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 277 RLFNSYGPTEAT-----VSASLAELRPGDPVTIGQ-PLPNYGLLV--VDAEMKLLPAGATGELCIIGPGVAEGYLGRPEL 348
Cdd:PRK07470 307 VLVQYFGLGEVTgnitvLPPALHDAEDGPDARIGTcGFERTGMEVqiQDDEGRELPPGETGEICVIGPAVFAGYYNNPEA 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 349 TAEKFLpNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV--VLKPVDG 426
Cdd:PRK07470 387 NAKAFR-DGW---------FRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAVSEVAVlgVPDPVWG 456
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 427 iDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK07470 457 -EVGVAVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKMVRE 514
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
143-486 |
1.27e-34 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 135.54 E-value: 1.27e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR------VYQ--GFSVafdmsfeeIWISYLVGATLWIAP 214
Cdd:cd17630 2 LATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSwllslpLYHvgGLAI--------LVRSLLAGAELVLLE 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 215 REitadpDALPLALARQGITVLHAVPTLLA------LFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTE-A 287
Cdd:cd17630 74 RN-----QALAEDLAPPGVTHVSLVPTQLQrlldsgQGPAALKSLRAVLLGGAPIPPELLERAADRGIPLYTTYGMTEtA 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 288 TVSASLAELRPGDPvTIGQPLPnygllvvDAEMKLLPagaTGELCIIGPGVAEGYL--GRPELTAEkflpNPWaehagep 365
Cdd:cd17630 149 SQVATKRPDGFGRG-GVGVLLP-------GRELRIVE---DGEIWVGGASLAMGYLrgQLVPEFNE----DGW------- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 366 rlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLkPVDGI---DQLVAYIVPGGGQPV 442
Cdd:cd17630 207 --FTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAAL-AAHPAVRDAFVV-GVPDEelgQRPVAVIVGRGPADP 282
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 2772088563 443 qiAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:cd17630 283 --AELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAW 324
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
2-485 |
4.20e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 138.48 E-value: 4.20e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK06145 4 LSASIAFHARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPIN 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLrEGLDAIDCPIWDQPELMQKvDGPLLRRGGAgPRHPAYV---------IYTSGS 152
Cdd:PRK06145 84 YRLAADEVAYILGDAGAKLLLVDEEF-DAIVALETPKIVIDAAAQA-DSRRLAQGGL-EIPPQAAvaptdlvrlMYTSGT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICHFLRSENERLGVRADDR---VYQGFSV-AFDM-SFEEIWisylVGATLWIApREItaDPDALPLA 227
Cdd:PRK06145 161 TDRPKGVMHSYGNLHWKSIDHVIALGLTASERllvVGPLYHVgAFDLpGIAVLW----VGGTLRIH-REF--DPEAVLAA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGITVLHAVPTLLALFGA-------DVPSLRLINLGGEICPPALVERWATPTR--RLFNSYGPTEATVSASLAEL-R 297
Cdd:PRK06145 234 IERHRLTCAWMAPVMLSRVLTvpdrdrfDLDSLAWCIGGGEKTPESRIRDFTRVFTraRYIDAYGLTETCSGDTLMEAgR 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGDPV-TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNpWaehageprlYRTGDLARI 376
Cdd:PRK06145 314 EIEKIgSTGRALAHVEIRIADGAGRWLPPNMKGEICMRGPKVTKGYWKDPEKTAEAFYGD-W---------FRSGDVGYL 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 377 DEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLKPVDGI--DQLVAYIVPGGGQPVQIAQLRAQLRET 454
Cdd:PRK06145 384 DEEGFLYLTDRKKDMIISGGENIASSEVERVIY-ELPEVAEAAVIGVHDDRwgERITAVVVLNPGATLTLEALDRHCRQR 462
|
490 500 510
....*....|....*....|....*....|.
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK06145 463 LASFKVPRQLKVRDELPRNPSGKVLKRVLRD 493
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
1-483 |
9.80e-34 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 137.26 E-value: 9.80e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTALV--CGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWL 78
Cdd:cd05923 2 TVFEMLRRAASRAPDACAIAdpARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 79 PFDADTPAERVMVCLEDAQAVGIVTGHGlREGLDAIDCPIWDQPELMQKVD-------GPLLRRGGAGPRHPAYVIYTSG 151
Cdd:cd05923 82 LINPRLKAAELAELIERGEMTAAVIAVD-AQVMDAIFQSGVRVLALSDLVGlgepesaGPLIEDPPREPEQPAFVFYTSG 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 152 STGKPKGVPINQGAICHFLRSENERLGVR--ADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITAdPDALPLaLA 229
Cdd:cd05923 161 TTGLPKGAVIPQRAAESRVLFMSTQAGLRhgRHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVVEEFDP-ADALKL-IE 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 230 RQGITVLHAVPTLL-ALF------GADVPSLRLINLGGEICPPALVERW--ATPTRRLfNSYGPTEATVSASLAELRPGd 300
Cdd:cd05923 239 QERVTSLFATPTHLdALAaaaefaGLKLSSLRHVTFAGATMPDAVLERVnqHLPGEKV-NIYGTTEAMNSLYMRDARTG- 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 301 pvTIGQPLPNYGLLVV---DAEMKLLPAGATGELCIIGPGVA--EGYLGRPELTAEKfLPNPWaehageprlYRTGDLAR 375
Cdd:cd05923 317 --TEMRPGFFSEVRIVrigGSPDEALANGEEGELIVAAAADAafTGYLNQPEATAKK-LQDGW---------YRTGDVGY 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRET- 454
Cdd:cd05923 385 VDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSADELDQFCRASe 464
|
490 500
....*....|....*....|....*....
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:cd05923 465 LADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
148-484 |
3.61e-33 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 132.02 E-value: 3.61e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 148 YTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRV------YQGFSVAFDmsfeeIWISYLVGATLwIAPrEITADP 221
Cdd:cd05917 9 FTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLcipvplFHCFGSVLG-----VLACLTHGATM-VFP-SPSFDP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLALARQGITVLHAVPTL-LALFGA------DVPSLRLINLGGEICPPALVerwatptRRLFN---------SYGPT 285
Cdd:cd05917 82 LAVLEAIEKEKCTALHGVPTMfIAELEHpdfdkfDLSSLRTGIMAGAPCPPELM-------KRVIEvmnmkdvtiAYGMT 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 286 EAtvSASLAELRPGDPV-----TIGQPLPNYGLLVVDAEMK-LLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWa 359
Cdd:cd05917 155 ET--SPVSTQTRTDDSIekrvnTVGRIMPHTEAKIVDPEGGiVPPVGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDGW- 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 360 ehageprlYRTGDLARIDEQGQIHCLGRADDQVkVRGfrvelG------EIEAALsrqlpNGTAAVVLKPVDGI------ 427
Cdd:cd05917 232 --------LHTGDLAVMDEDGYCRIVGRIKDMI-IRG-----GeniyprEIEEFL-----HTHPKVSDVQVVGVpderyg 292
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 428 DQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05917 293 EEVCAWIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
10-485 |
4.31e-33 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 136.99 E-value: 4.31e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGALS------LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA----WLP 79
Cdd:TIGR02188 67 LEARPDKVAIIWEGDEpgevrkITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIhsvvFGG 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 FDADTPAERVMvcleDAQAVGIVTGHGLREG-----LDAI-DCPIWDQPELMQKVdgPLLRRGGA--------------- 138
Cdd:TIGR02188 147 FSAEALADRIN----DAGAKLVITADEGLRGgkvipLKAIvDEALEKCPVSVEHV--LVVRRTGNpvvpwvegrdvwwhd 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 139 --------------GPRHPAYVIYTSGSTGKPKGVPINQGA-ICHFLRSENERLGVRADDRvyqgFSVAFDMSfeeiWI- 202
Cdd:TIGR02188 221 lmakasaycepepmDSEDPLFILYTSGSTGKPKGVLHTTGGyLLYAAMTMKYVFDIKDGDI----FWCTADVG----WIt 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 203 --SYLV------GATLWI---APReiTADPDALPLALARQGITVLHAVPTLLALFGA---------DVPSLRL------- 255
Cdd:TIGR02188 293 ghSYIVygplanGATTVMfegVPT--YPDPGRFWEIIEKHKVTIFYTAPTAIRALMRlgdewvkkhDLSSLRLlgsvgep 370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 256 IN----------LGGEICPpaLVER-WATPTrrlfnsyGPTEATVSASLAELRPGdpvTIGQPLPNYGLLVVDAEMKLLP 324
Cdd:TIGR02188 371 INpeawmwyykvVGKERCP--IVDTwWQTET-------GGIMITPLPGATPTKPG---SATLPFFGIEPAVVDEEGNPVE 438
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 325 -AGATGELCIIG--PGVAEGYLGRPeltaEKFLPNPWAEHagePRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVEL 401
Cdd:TIGR02188 439 gPGEGGYLVIKQpwPGMLRTIYGDH----ERFVDTYFSPF---PGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGT 511
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 402 GEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIV--PGGGQPVQIAQ-LRAQLRETLPPYMVPSRFELVDTIPRLTSGK 477
Cdd:TIGR02188 512 AEIESALVSHPAVAEAAVVGIPDDIKGQaIYAFVTlkDGYEPDDELRKeLRKHVRKEIGPIAKPDKIRFVPGLPKTRSGK 591
|
....*...
gi 2772088563 478 IDRKALRA 485
Cdd:TIGR02188 592 IMRRLLRK 599
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
2-408 |
1.81e-32 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 134.84 E-value: 1.81e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVC----GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAW 77
Cdd:COG1022 13 LPDLLRRRAARFPDRVALREkedgIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVT 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 78 LPFDADTPAERVMVCLEDAQAVGIVTG--------HGLREGLDAIDCPIWDQPELMQKVDGPL----LRRGGAGPRHPAY 145
Cdd:COG1022 93 VPIYPTSSAEEVAYILNDSGAKVLFVEdqeqldklLEVRDELPSLRHIVVLDPRGLRDDPRLLsldeLLALGREVADPAE 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 V---------------IYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVyqgfsVAF---DMSFEEIWISYLV- 206
Cdd:COG1022 173 LearraavkpddlatiIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRT-----LSFlplAHVFERTVSYYALa 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 207 -GATLWIAPreitaDPDALPLALARQGITVLHAVPTLL-------------------ALF----------------GADV 250
Cdd:COG1022 248 aGATVAFAE-----SPDTLAEDLREVKPTFMLAVPRVWekvyagiqakaeeagglkrKLFrwalavgrryararlaGKSP 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 251 PSL---------RLI------NLGGEI---------CPPALVerwatptrRLFNS--------YGPTEATVSAS---LAE 295
Cdd:COG1022 323 SLLlrlkhaladKLVfsklreALGGRLrfavsggaaLGPELA--------RFFRAlgipvlegYGLTETSPVITvnrPGD 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 296 LRPGdpvTIGQPLPNygllvvdAEMKLlpaGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLAR 375
Cdd:COG1022 395 NRIG---TVGPPLPG-------VEVKI---AEDGEILVRGPNVMKGYYKNPEATAEAFDADGW---------LHTGDIGE 452
|
490 500 510
....*....|....*....|....*....|....
gi 2772088563 376 IDEQGQIHCLGRADDQVKVR-GFRVELGEIEAAL 408
Cdd:COG1022 453 LDEDGFLRITGRKKDLIVTSgGKNVAPQPIENAL 486
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
2-486 |
2.82e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 132.42 E-value: 2.82e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPD-QTALVCGALSLSYRELDERASLMAHhlikqGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPF 80
Cdd:PRK07787 1 LASLNPAAVAAAADiADAVRIGGRVLSRSDLAGAATAVAE-----RVAGARRVAVLATPTLATVLAVVGALIAGVPVVPV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADT-PAERvmvcledaqavgivtGHGLRE-GLDAIDCPIWDQPELMQKVDGPLLRRG-----GAGPRHPAYVIYTSGST 153
Cdd:PRK07787 76 PPDSgVAER---------------RHILADsGAQAWLGPAPDDPAGLPHVPVRLHARSwhrypEPDPDAPALIVYTSGTT 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 154 GKPKGVPINQGAICHFLRSENERLGVRADDRVYQG---FSVafdmsfeEIWISYLVGAtLWIAPREI---TADPDALPLA 227
Cdd:PRK07787 141 GPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGlplFHV-------HGLVLGVLGP-LRIGNRFVhtgRPTPEAYAQA 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGiTVLHAVPTLLALFGAD------VPSLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEA--TVSASL-AELR 297
Cdd:PRK07787 213 LSEGG-TLYFGVPTVWSRIAADpeaaraLRGARLLVSGSAALPVPVFDRLAALTgHRPVERYGMTETliTLSTRAdGERR 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGdpvTIGQPLPNYGLLVVDAEMKLLPA-GAT-GELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLAR 375
Cdd:PRK07787 292 PG---WVGLPLAGVETRLVDEDGGPVPHdGETvGELQVRGPTLFDGYLNRPDATAAAFTADGW---------FRTGDVAV 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGR-ADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVPGGgqPVQIAQLRAQLRE 453
Cdd:PRK07787 360 VDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQrIVAYVVGAD--DVAADELIDFVAQ 437
|
490 500 510
....*....|....*....|....*....|...
gi 2772088563 454 TLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK07787 438 QLSVHKRPREVRFVDALPRNAMGKVLKKQLLSE 470
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
11-485 |
5.65e-32 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 133.59 E-value: 5.65e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 11 ASQPDQTAL-----VCGAL-SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGA----AWLPF 80
Cdd:cd05967 62 AGRGDQIALiydspVTGTErTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAihsvVFGGF 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADTPAERVmvclEDAQAVGIVTGH-GLREG--------LD-AI--------DCPIWDQPELMQKVDGPLL------RRG 136
Cdd:cd05967 142 AAKELASRI----DDAKPKLIVTAScGIEPGkvvpykplLDkALelsghkphHVLVLNRPQVPADLTKPGRdldwseLLA 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 137 GAGPR--------HPAYVIYTSGSTGKPKGVPINQGAICHFLR-SENERLGVRADDrVYQGFSvafDMSfeeiWI---SY 204
Cdd:cd05967 218 KAEPVdcvpvaatDPLYILYTSGTTGKPKGVVRDNGGHAVALNwSMRNIYGIKPGD-VWWAAS---DVG----WVvghSY 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 LV------GAT--LWIAPREITADPDALPLALARQGITVLHAVPTLL-----------ALFGADVPSLRLINLGGEICPP 265
Cdd:cd05967 290 IVygpllhGATtvLYEGKPVGTPDPGAFWRVIEKYQVNALFTAPTAIrairkedpdgkYIKKYDLSSLRTLFLAGERLDP 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 266 ALVErWATPT--RRLFNSYGPTEA----TVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGP--- 336
Cdd:cd05967 370 PTLE-WAENTlgVPVIDHWWQTETgwpiTANPVGLEPLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPlpp 448
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 337 GVAEGYLGRPELTAEKFLpnpwaehAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGT 416
Cdd:cd05967 449 GCLLTLWKNDERFKKLYL-------SKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAE 521
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 417 AAVVLKP--VDGIDQLVAYIVPGGGQPVQIA---QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05967 522 CAVVGVRdeLKGQVPLGLVVLKEGVKITAEElekELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRK 595
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
26-484 |
8.62e-32 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 130.33 E-value: 8.62e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP-FDADTPaERVMVCLEDAQAVGIVTG 104
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPlFTAFGP-KAIEHRLRTSGARLVVTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 HGLREGLDaidcpiwdqpelmqkvDGPLLrrggagprhpayVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR 184
Cdd:cd05973 80 AANRHKLD----------------SDPFV------------MMFTSGTTGLPKGVPVPLRALAAFGAYLRDAVDLRPEDS 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 185 vyqgFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLA---LARQGITVLHAVPT---LLALFGADVP-----SL 253
Cdd:cd05973 132 ----FWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTwrvIERLGVTNLAGSPTayrLLMAAGAEVParpkgRL 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 254 RLINLGGEICPPALVeRWATPTRRL--FNSYGPTEATVSASLAElRPGDPV---TIGQPLPNYGLLVVDAEMKLLPAGAT 328
Cdd:cd05973 208 RRVSSAGEPLTPEVI-RWFDAALGVpiHDHYGQTELGMVLANHH-ALEHPVhagSAGRAMPGWRVAVLDDDGDELGPGEP 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 329 GELCIigpGVAEGylgrPELTAEKFLPNPWAEHAGepRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAAL 408
Cdd:cd05973 286 GRLAI---DIANS----PLMWFRGYQLPDTPAIDG--GYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESAL 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 409 SRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGG---QPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05973 357 IEHPAVAEAAVIGVPDPERTEVVkAFVVLRGGhegTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLR 436
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
18-484 |
1.01e-31 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 131.36 E-value: 1.01e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 18 ALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQ 97
Cdd:PRK12406 4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 98 AVGIVTG----HGLREGLDAiDCPIWD---QPELMQ--KVDgPLLRRGGAG--------PRHPAY----------VIYTS 150
Cdd:PRK12406 84 ARVLIAHadllHGLASALPA-GVTVLSvptPPEIAAayRIS-PALLTPPAGaidwegwlAQQEPYdgppvpqpqsMIYTS 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 151 GSTGKPKGVPINQGAICHFLRSENER-------LGVRA--DDRVYQGFSVAFDMSfeeiwiSYLVGATLWIAPReitADP 221
Cdd:PRK12406 162 GTTGHPKGVRRAAPTPEQAAAAEQMRaliyglkPGIRAllTGPLYHSAPNAYGLR------AGRLGGVLVLQPR---FDP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLALARQGITVLHAVPT----LLALFGA-----DVPSLRLINLGGEICPP----ALVERWAtPTrrLFNSYGPTE-- 286
Cdd:PRK12406 233 EELLQLIERHRITHMHMVPTmfirLLKLPEEvrakyDVSSLRHVIHAAAPCPAdvkrAMIEWWG-PV--IYEYYGSTEsg 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 287 --ATVSASLAELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAE-GYLGRPELTAEkflpnpwAEHAG 363
Cdd:PRK12406 310 avTFATSEDALSHPG---TVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGNPDfTYHNKPEKRAE-------IDRGG 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 364 eprLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLkpvdGI------DQLVAYIVPG 437
Cdd:PRK12406 380 ---FITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLH-AVPGVHDCAVF----GIpdaefgEALMAVVEPQ 451
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 2772088563 438 GGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK12406 452 PGATLDEADIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
10-485 |
1.69e-31 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 131.91 E-value: 1.69e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGA------LSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA----WLP 79
Cdd:cd05966 63 LKERGDKVAIIWEGdepdqsRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVhsvvFAG 142
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 FDADTPAERVmvclEDAQAVGIVTGHGLREGLDAIdcpiwdqpELMQKVDGPL-----------LRRGGAG--------- 139
Cdd:cd05966 143 FSAESLADRI----NDAQCKLVITADGGYRGGKVI--------PLKEIVDEALekcpsvekvlvVKRTGGEvpmtegrdl 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 140 -------------------PRHPAYVIYTSGSTGKPKGVPINQGAichFLrsenerLGVraddrvYQGFSVAFDMSFEEI 200
Cdd:cd05966 211 wwhdlmakqspecepewmdSEDPLFILYTSGSTGKPKGVVHTTGG---YL------LYA------ATTFKYVFDYHPDDI 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 201 --------WI---SYLV------GATLWI---APReiTADPDALPLALARQGITVLHAVPT---LLALFGADVP------ 251
Cdd:cd05966 276 ywctadigWItghSYIVygplanGATTVMfegTPT--YPDPGRYWDIVEKHKVTIFYTAPTairALMKFGDEWVkkhdls 353
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 SLRL-------IN----------LGGEICPpaLVER-WATPTrrlfnsyGPTEATVSASLAELRPGdpvTIGQPLPNYGL 313
Cdd:cd05966 354 SLRVlgsvgepINpeawmwyyevIGKERCP--IVDTwWQTET-------GGIMITPLPGATPLKPG---SATRPFFGIEP 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 314 LVVDAEMKLLPAGATGELCIIG--PGVAEGYLGRPEltaeKFLPNPWAEHAGeprLYRTGDLARIDEQGQIHCLGRADDQ 391
Cdd:cd05966 422 AILDEEGNEVEGEVEGYLVIKRpwPGMARTIYGDHE----RYEDTYFSKFPG---YYFTGDGARRDEDGYYWITGRVDDV 494
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 392 VKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVP-GGGQPVQ--IAQLRAQLRETLPPYMVPSRFELV 467
Cdd:cd05966 495 INVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEaIYAFVTLkDGEEPSDelRKELRKHVRKEIGPIATPDKIQFV 574
|
570
....*....|....*...
gi 2772088563 468 DTIPRLTSGKIDRKALRA 485
Cdd:cd05966 575 PGLPKTRSGKIMRRILRK 592
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
7-502 |
3.95e-31 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 131.69 E-value: 3.95e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 7 EATAASQPDQTALVCGALsLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLasQLAIAKTGAAWLPFDADTPA 86
Cdd:PRK06060 13 QASEAGWYDRPAFYAADV-VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLV--QLLLACLARGVMAFLANPEL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ERVMVCLE--DAQAVGIVTGHGLREGLDAIDcpIWDQPELMQKVdgpllRRGGAGPRHP------AYVIYTSGSTGKPKG 158
Cdd:PRK06060 90 HRDDHALAarNTEPALVVTSDALRDRFQPSR--VAEAAELMSEA-----ARVAPGGYEPmggdalAYATYTSGTTGPPKA 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 159 V--------PINQGAICHFLRSENERLGVrADDRVYQGFSVAfdmsfEEIWISYLVGATLWIAPREITADPDALplALAR 230
Cdd:PRK06060 163 AihrhadplTFVDAMCRKALRLTPEDTGL-CSARMYFAYGLG-----NSVWFPLATGGSAVINSAPVTPEAAAI--LSAR 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 231 QGITVLHAVPTLLA-LFGADVP----SLRLINLGGEICPPALVERW-----ATPtrrLFNSYGPTEAT---VSASLAELR 297
Cdd:PRK06060 235 FGPSVLYGVPNFFArVIDSCSPdsfrSLRCVVSAGEALELGLAERLmeffgGIP---ILDGIGSTEVGqtfVSNRVDEWR 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPE--LTAEKFLpnpwaehageprlyRTGDLAR 375
Cdd:PRK06060 312 LG---TLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVRGPAIAKGYWNRPDspVANEGWL--------------DTRDRVC 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVV-LKPVDGIDQLVAYIVPGGGQPVQIAQLR---AQL 451
Cdd:PRK06060 375 IDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVaVRESTGASTLQAFLVATSGATIDGSVMRdlhRGL 454
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 452 RETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW----PMAALSATErSESDLP 502
Cdd:PRK06060 455 LNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALRKQsptkPIWELSLTE-PGSGVR 508
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
27-484 |
4.83e-31 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 129.51 E-value: 4.83e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGH 105
Cdd:cd05928 43 SFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSD 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 GLREGLDAI--DCPIWDQPELM--QKVDG-----PLLRRggAGPRH---------PAYVIYTSGSTGKPKGVPINQGAIC 167
Cdd:cd05928 123 ELAPEVDSVasECPSLKTKLLVseKSRDGwlnfkELLNE--ASTEHhcvetgsqePMAIYFTSGTTGSPKMAEHSHSSLG 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 168 HFLRSENER-LGVRADDRVYQ----GFSVAFDMSFEEIWISylvGATLWI--APReitADPDALPLALARQGITVLHAVP 240
Cdd:cd05928 201 LGLKVNGRYwLDLTASDIMWNtsdtGWIKSAWSSLFEPWIQ---GACVFVhhLPR---FDPLVILKTLSSYPITTFCGAP 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 241 TLLALFGAD------VPSLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEATVSASL---AELRPGdpvTIGQPLPN 310
Cdd:cd05928 275 TVYRMLVQQdlssykFPSLQHCVTGGEPLNPEVLEKWKAQTgLDIYEGYGQTETGLICANfkgMKIKPG---SMGKASPP 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 311 YGLLVVDAEMKLLPAGATGELCI-IGP----GVAEGYLGRPELTAEKFLPNpwaehageprLYRTGDLARIDEQGQIHCL 385
Cdd:cd05928 352 YDVQIIDDNGNVLPPGTEGDIGIrVKPirpfGLFSGYVDNPEKTAATIRGD----------FYLTGDRGIMDEDGYFWFM 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 386 GRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvDGIDQLV--AYIVPG----GGQPVQ-IAQLRAQLRETLPPY 458
Cdd:cd05928 422 GRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSP-DPIRGEVvkAFVVLApqflSHDPEQlTKELQQHVKSVTAPY 500
|
490 500
....*....|....*....|....*.
gi 2772088563 459 MVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05928 501 KYPRKVEFVQELPKTVTGKIQRNELR 526
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
146-478 |
6.38e-31 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 124.92 E-value: 6.38e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 VIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDR--VYQGFSVAFdmSFEEIWISYLV-GATlwIAPrEITADPD 222
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRylIINPFFHTF--GYKAGIVACLLtGAT--VVP-VAVFDVD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 223 ALPLALARQGITVLHAVPTL-LALFG------ADVPSLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEATVsASL 293
Cdd:cd17638 80 AILEAIERERITVLPGPPTLfQSLLDhpgrkkFDLSSLRAAVTGAATVPVELVRRMRSelGFETVLTAYGLTEAGV-ATM 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 294 AelRPGDPV-----TIGQPLPNYGLLVVDAemkllpagatGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlY 368
Cdd:cd17638 159 C--RPGDDAetvatTCGRACPGFEVRIADD----------GEVLVRGYNVMQGYLDDPEATAEAIDADGW---------L 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 369 RTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQL 447
Cdd:cd17638 218 HTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVgKAFVVARPGVTLTEEDV 297
|
330 340 350
....*....|....*....|....*....|.
gi 2772088563 448 RAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:cd17638 298 IAWCRERLANYKVPRFVRFLDELPRNASGKV 328
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
2-478 |
7.04e-31 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 129.31 E-value: 7.04e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPF 80
Cdd:PRK08314 12 LFHNLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADTPAERVMVCLEDAQAVGIVTGHGLREGL------------------DAIDcpiwDQPE------LMQKVDGPLLRRG 136
Cdd:PRK08314 92 NPMNREEELAHYVTDSGARVAIVGSELAPKVapavgnlrlrhvivaqysDYLP----AEPEiavpawLRAEPPLQALAPG 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 137 G------------------AGPRHPAYVIYTSGSTGKPKGvpinqgaiC-HFLRSENERLgvraddrvyQGFSVAFDMSF 197
Cdd:PRK08314 168 GvvawkealaaglappphtAGPDDLAVLPYTSGTTGVPKG--------CmHTHRTVMANA---------VGSVLWSNSTP 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 198 EEIWISYL-----------------VGATLWIAPReitADPDALPLALARQGITVLHAVPTLLALFGA-------DVPSL 253
Cdd:PRK08314 231 ESVVLAVLplfhvtgmvhsmnapiyAGATVVLMPR---WDREAAARLIERYRVTHWTNIPTMVVDFLAspglaerDLSSL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 254 RLINLGGEICPPALVER-WATPTRRLFNSYGPTEaTVSASLAElrPGD---PVTIGQPLPNYGLLVVDAEM-KLLPAGAT 328
Cdd:PRK08314 308 RYIGGGGAAMPEAVAERlKELTGLDYVEGYGLTE-TMAQTHSN--PPDrpkLQCLGIPTFGVDARVIDPETlEELPPGEV 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 329 GELCIIGPGVAEGYLGRPELTAEKFLpnpwaEHAGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAAL 408
Cdd:PRK08314 385 GEIVVHGPQVFKGYWNRPEATAEAFI-----EIDGK-RFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLL 458
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 409 SRQLPNGTAAVVLKPVDGIDQLV-AYIVPGGGQPVQIA--QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:PRK08314 459 YKHPAIQEACVIATPDPRRGETVkAVVVLRPEARGKTTeeEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
10-489 |
1.94e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 127.23 E-value: 1.94e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALV--CGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAE 87
Cdd:PRK09088 5 ARLQPQRLAAVdlALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSAS 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 88 RVMVCLEDAQAVGIVTGhglrEGLDAIDCPIWDQPELMQKVDG--PLLRRGgAGPRHPAYVIYTSGSTGKPKGVPINQGA 165
Cdd:PRK09088 85 ELDALLQDAEPRLLLGD----DAVAAGRTDVEDLAAFIASADAlePADTPS-IPPERVSLILFTSGTSGQPKGVMLSERN 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 166 ICHflRSENERLGVRADDRvyQGFSVAFDMsFEEIWI------SYLVGATLWIAPreiTADPDALPLALARQGITVLH-- 237
Cdd:PRK09088 160 LQQ--TAHNFGVLGRVDAH--SSFLCDAPM-FHIIGLitsvrpVLAVGGSILVSN---GFEPKRTLGRLGDPALGITHyf 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 238 AVPTLLALF-------GADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEA------TVSASLAELRPGdpvTI 304
Cdd:PRK09088 232 CVPQMAQAFraqpgfdAAALRHLTALFTGGAPHAAEDILGWLDDGIPMVDGFGMSEAgtvfgmSVDCDVIRAKAG---AA 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 305 GQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHC 384
Cdd:PRK09088 309 GIPTPTVQTRVVDDQGNDCPAGVPGELLLRGPNLSPGYWRRPQATARAFTGDGW---------FRTGDIARRDADGFFWV 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 385 LGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSR 463
Cdd:PRK09088 380 VDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVgYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKH 459
|
490 500
....*....|....*....|....*.
gi 2772088563 464 FELVDTIPRLTSGKIDRKALRAWPMA 489
Cdd:PRK09088 460 LRLVDALPRTASGKLQKARLRDALAA 485
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
2-484 |
2.18e-30 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 127.49 E-value: 2.18e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGAL-----SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA 76
Cdd:PRK08008 9 LRQMWDDLADVYGHKTALIFESSggvvrRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 77 WLPFDADTPAERVMVCLEDAQAVGIVT---------------GHGL------REGLDAIDcPIWDQPELMQKVDGPLLRR 135
Cdd:PRK08008 89 MVPINARLLREESAWILQNSQASLLVTsaqfypmyrqiqqedATPLrhicltRVALPADD-GVSSFTQLKAQQPATLCYA 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 136 GGAGPRHPAYVIYTSGSTGKPKGVpinqgAICHFlrseNERLG---------VRADDRvYQGFSVAFDMSFE--EIWISY 204
Cdd:PRK08008 168 PPLSTDDTAEILFTSGTTSRPKGV-----VITHY----NLRFAgyysawqcaLRDDDV-YLTVMPAFHIDCQctAAMAAF 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 LVGATL-----------WIAPREITAdpdalplalarqgiTVLHAVPTLLALFGADVPS-------LR----LINLGGEi 262
Cdd:PRK08008 238 SAGATFvllekysarafWGQVCKYRA--------------TITECIPMMIRTLMVQPPSandrqhcLRevmfYLNLSDQ- 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 263 cppalvERWATPTR---RLFNSYGPTEATVSasLAELRPGDP---VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIG- 335
Cdd:PRK08008 303 ------EKDAFEERfgvRLLTSYGMTETIVG--IIGDRPGDKrrwPSIGRPGFCYEAEIRDDHNRPLPAGEIGEICIKGv 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 336 PG--VAEGYLGRPELTAEKFLPNPWaehageprLYrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQlP 413
Cdd:PRK08008 375 PGktIFKEYYLDPKATAKVLEADGW--------LH-TGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATH-P 444
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 414 NGTAAVVLKPVDGI-DQLV-AYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK08008 445 KIQDIVVVGIKDSIrDEAIkAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
2-491 |
1.21e-29 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 128.50 E-value: 1.21e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALV-CGALSLSYRELDERASLMAHhLIKQGVRPGQIVGLWLPR--------------------- 59
Cdd:PRK08633 617 LAEAWIDTAKRNWSRLAVAdSTGGELSYGKALTGALALAR-LLKRELKDEENVGILLPPsvagalanlalllagkvpvnl 695
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 60 ----GIDLLASQLAIAK-----TGAAWL------PFDADTPAERVMVCLEDAQAvGIVTGHGLREGLDAIDCPIWdqpel 124
Cdd:PRK08633 696 nytaSEAALKSAIEQAQiktviTSRKFLeklknkGFDLELPENVKVIYLEDLKA-KISKVDKLTALLAARLLPAR----- 769
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 125 mqkvdgpLLRRGGAGPRHP---AYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQ--------GFSVAF 193
Cdd:PRK08633 770 -------LLKRLYGPTFKPddtATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSslpffhsfGLTVTL 842
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 194 dmsfeeiWISYLVGatlwiaPREIT-ADP-DALPLA--LARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEI 262
Cdd:PRK08633 843 -------WLPLLEG------IKVVYhPDPtDALGIAklVAKHRATILLGTPTFLRLYlrnkklhPLMFASLRLVVAGAEK 909
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 263 CPPALVERWATP-TRRLFNSYGPTEATVSASLA---ELRPGDPV-------TIGQPLPNYGLLVVDAE-MKLLPAGATGE 330
Cdd:PRK08633 910 LKPEVADAFEEKfGIRILEGYGATETSPVASVNlpdVLAADFKRqtgskegSVGMPLPGVAVRIVDPEtFEELPPGEDGL 989
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 331 LCIIGPGVAEGYLGRPELTAEKFlpnpwaEHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSR 410
Cdd:PRK08633 990 ILIGGPQVMKGYLGDPEKTAEVI------KDIDGIGWYVTGDKGHLDEDGFLTITDRYSRFAKIGGEMVPLGAVEEELAK 1063
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 411 QLPNGTAAVVlkpVDGID------QLVAYIVPGGGQPvqiAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:PRK08633 1064 ALGGEEVVFA---VTAVPdekkgeKLVVLHTCGAEDV---EELKRAIKESgLPNLWKPSRYFKVEALPLLGSGKLDLKGL 1137
|
....*...
gi 2772088563 484 RAWPMAAL 491
Cdd:PRK08633 1138 KELALALL 1145
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
25-485 |
3.06e-29 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 124.91 E-value: 3.06e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTG 104
Cdd:cd05968 91 TLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKALITA 170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 HG---------LREGLD--AIDCPIWDQ---------PELMQKVD---------GPLLRRGGAGPRHPAYVIYTSGSTGK 155
Cdd:cd05968 171 DGftrrgrevnLKEEADkaCAQCPTVEKvvvvrhlgnDFTPAKGRdlsydeekeTAGDGAERTESEDPLMIIYTSGTTGK 250
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 156 PKGV-------PINQGAICHFLrsenerLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWI---APREitADPDALP 225
Cdd:cd05968 251 PKGTvhvhagfPLKAAQDMYFQ------FDLKPGDLLTWFTDLGWMMGPWLIFGGLILGATMVLydgAPDH--PKADRLW 322
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 226 LALARQGITVLHAVPTLL-ALFGA--------DVPSLRLINLGGEICPPA----LVERWATPTRRLFNSYGPTEatVSAS 292
Cdd:cd05968 323 RMVEDHEITHLGLSPTLIrALKPRgdapvnahDLSSLRVLGSTGEPWNPEpwnwLFETVGKGRNPIINYSGGTE--ISGG 400
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 293 L---AELRPGDPVTIGQPLPNYGLLVVDAEMKLLPaGATGELCIIGP--GVAEGYLGRPELTAEKF---LPNPWAEhage 364
Cdd:cd05968 401 IlgnVLIKPIKPSSFNGPVPGMKADVLDESGKPAR-PEVGELVLLAPwpGMTRGFWRDEDRYLETYwsrFDNVWVH---- 475
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 365 prlyrtGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAAL-SRQLPNGTAAV-VLKPVDGiDQLVAYIV--PGGGQ 440
Cdd:cd05968 476 ------GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLnAHPAVLESAAIgVPHPVKG-EAIVCFVVlkPGVTP 548
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2772088563 441 PVQIA-QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05968 549 TEALAeELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRA 594
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
2-490 |
5.27e-29 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 123.72 E-value: 5.27e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK06155 23 LPAMLARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPIN 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMqKVDGPLLRR-------------------GGAGPRH 142
Cdd:PRK06155 103 TALRGPQLEHILRNSGARLLVVEAALLAALEAADPGDLPLPAVW-LLDAPASVSvpagwstaplppldapapaAAVQPGD 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSV----AFDMSFEEIwisyLVGATLWIAPReIT 218
Cdd:PRK06155 182 TAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLfhtnALNAFFQAL----LAGATYVLEPR-FS 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 219 AdpDALPLALARQGITVLHavptllaLFGADVPSL------------RL-INLGGEIcPPALV----ERWATPtrrLFNS 281
Cdd:PRK06155 257 A--SGFWPAVRRHGATVTY-------LLGAMVSILlsqparesdrahRVrVALGPGV-PAALHaafrERFGVD---LLDG 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 282 YGPTEATV--SASLAELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIG--PG-VAEGYLGRPELTAEKFlPN 356
Cdd:PRK06155 324 YGSTETNFviAVTHGSQRPG---SMGRLAPGFEARVVDEHDQELPDGEPGELLLRAdePFaFATGYFGMPEKTVEAW-RN 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 357 PWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVD-GIDQLVAYIV 435
Cdd:PRK06155 400 LW---------FHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSElGEDEVMAAVV 470
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 436 PGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAA 490
Cdd:PRK06155 471 LRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQGVTA 525
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
2-507 |
3.00e-28 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 121.46 E-value: 3.00e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVC--GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawlp 79
Cdd:PRK08315 18 IGQLLDRTAARYPDREALVYrdQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGA---- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 fdadtpaerVMVC---------LE------DAQAVGIVTG------------------HGLREGLDAIDCP-----IW-- 119
Cdd:PRK08315 94 ---------ILVTinpayrlseLEyalnqsGCKALIAADGfkdsdyvamlyelapelaTCEPGQLQSARLPelrrvIFlg 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 120 -DQPELMQKVDGpLLRRGGAGPR----------HPAYVI---YTSGSTGKPKGVP------INQGaichFLRSENERLGv 179
Cdd:PRK08315 165 dEKHPGMLNFDE-LLALGRAVDDaelaarqatlDPDDPIniqYTSGTTGFPKGATlthrniLNNG----YFIGEAMKLT- 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 180 rADDRV------YQGF--------SVAfdmsfeeiwisylVGATLwIAPREITaDPDALPLALARQGITVLHAVPTL--- 242
Cdd:PRK08315 239 -EEDRLcipvplYHCFgmvlgnlaCVT-------------HGATM-VYPGEGF-DPLATLAAVEEERCTALYGVPTMfia 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 243 ---LALFGA-DVPSLRLINLGGEICPPALVERwatpTRRLFN------SYGPTEAT-VSaslAELRPGDPV-----TIGQ 306
Cdd:PRK08315 303 eldHPDFARfDLSSLRTGIMAGSPCPIEVMKR----VIDKMHmsevtiAYGMTETSpVS---TQTRTDDPLekrvtTVGR 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 307 PLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCL 385
Cdd:PRK08315 376 ALPHLEVKIVDPETgETVPRGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGW---------MHTGDLAVMDEEGYVNIV 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 386 GRADDQVkVRGfrvelG------EIEAALSRQLPNGTAAVVlkpvdGI------DQLVAYIVPGGGQPVQIAQLRAQLRE 453
Cdd:PRK08315 447 GRIKDMI-IRG-----GeniyprEIEEFLYTHPKIQDVQVV-----GVpdekygEEVCAWIILRPGATLTEEDVRDFCRG 515
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 454 TLPPYMVPSRFELVDTIPRLTSGKIDRKALRawpmaalsatERSESDLPETEAE 507
Cdd:PRK08315 516 KIAHYKIPRYIRFVDEFPMTVTGKIQKFKMR----------EMMIEELGLQAAK 559
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
26-485 |
4.34e-28 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 120.85 E-value: 4.34e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP---FDADTPAERVMVCLEDA-----Q 97
Cdd:cd05906 40 QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPltvPPTYDEPNARLRKLRHIwqllgS 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 98 AVgIVTGHGLREGLDAIDCPiwdQPELMQKVDGPLLRRGGAGPRH--------PAYVIYTSGSTGKPKGVPINQGAICHF 169
Cdd:cd05906 120 PV-VLTDAELVAEFAGLETL---SGLPGIRVLSIEELLDTAADHDlpqsrpddLALLMLTSGSTGFPKAVPLTHRNILAR 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 170 LRSENERLGVRADDRVYQGfsVAFD----MSFEEIWISYLVGATLWIAPREITADPdALPLALA-RQGITVLHAVPTLLA 244
Cdd:cd05906 196 SAGKIQHNGLTPQDVFLNW--VPLDhvggLVELHLRAVYLGCQQVHVPTEEILADP-LRWLDLIdRYRVTITWAPNFAFA 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 245 LFGADVP----------SLRLINLGGE--ICPPA-----LVERWATPTRRLFNSYGPTE----ATVSASLAELRPGDP-- 301
Cdd:cd05906 273 LLNDLLEeiedgtwdlsSLRYLVNAGEavVAKTIrrllrLLEPYGLPPDAIRPAFGMTEtcsgVIYSRSFPTYDHSQAle 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 302 -VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEqG 380
Cdd:cd05906 353 fVSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGW---------FRTGDLGFLDN-G 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 381 QIHCLGRADDQVKVRGFRVELGEIEAALSRQ---LPNGTAAV-VLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETL- 455
Cdd:cd05906 423 NLTITGRTKDTIIVNGVNYYSHEIEAAVEEVpgvEPSFTAAFaVRDPGAETEELAIFFVPEYDLQDALSETLRAIRSVVs 502
|
490 500 510
....*....|....*....|....*....|....*.
gi 2772088563 456 ------PPYMVPSRFELvdtIPRLTSGKIDRKALRA 485
Cdd:cd05906 503 revgvsPAYLIPLPKEE---IPKTSLGKIQRSKLKA 535
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
26-486 |
1.10e-27 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 118.33 E-value: 1.10e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIvtgh 105
Cdd:cd05910 3 LSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAF---- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 glregldaIDCPIWDQpelmqkvdgpllrrggagprhPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRV 185
Cdd:cd05910 79 --------IGIPKADE---------------------PAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVD 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 186 YQGFSVaFDMsfeeiwISYLVGATLWIAP----REITADPDALPLALARQGITVLHAVPTLLALFGA-------DVPSLR 254
Cdd:cd05910 130 LATFPL-FAL------FGPALGLTSVIPDmdptRPARADPQKLVGAIRQYGVSIVFGSPALLERVARycaqhgiTLPSLR 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 255 LINLGGEICPPALVERWA---TPTRRLFNSYGPTEATVSASLA--ELR--------PGDPVTIGQPLPNYGLLVVDA--- 318
Cdd:cd05910 203 RVLSAGAPVPIALAARLRkmlSDEAEILTPYGATEALPVSSIGsrELLatttaatsGGAGTCVGRPIPGVRVRIIEIdde 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 319 ------EMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPwaehaGEPRLYRTGDLARIDEQGQIHCLGRADDQV 392
Cdd:cd05910 283 piaewdDTLELPRGEIGEITVTGPTVTPTYVNRPVATALAKIDDN-----SEGFWHRMGDLGYLDDEGRLWFCGRKAHRV 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 393 KVRGFRVELGEIEAalsrqlpngtaavVLKPVDGIDQ--LVAYIVPGGGQPV-----------QIAQLRAQLRETLPPYM 459
Cdd:cd05910 358 ITTGGTLYTEPVER-------------VFNTHPGVRRsaLVGVGKPGCQLPVlcveplpgtitPRARLEQELRALAKDYP 424
|
490 500 510
....*....|....*....|....*....|...
gi 2772088563 460 VPSRFELV------DTIPRLTSgKIDRKALRAW 486
Cdd:cd05910 425 HTQRIGRFlihpsfPVDIRHNA-KIFREKLAVW 456
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
2-485 |
2.01e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 118.88 E-value: 2.01e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFD 81
Cdd:PRK07788 51 FAGLVAHAARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLN 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 ADTPA---------ERVMVCLEDAQAVGIVTG--------HGLREGLDAIDCPIWDQPELMQKVDGPLLRRGGAGPRHPA 144
Cdd:PRK07788 131 TGFSGpqlaevaarEGVKALVYDDEFTDLLSAlppdlgrlRAWGGNPDDDEPSGSTDETLDDLIAGSSTAPLPKPPKPGG 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 145 YVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLwIAPREItaDPDAL 224
Cdd:PRK07788 211 IVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHATGWAHLTLAMALGSTV-VLRRRF--DPEAT 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 225 PLALARQGITVLHAVPTLL---------ALFGADVPSLRLINLGGEICPPALVERwATPT--RRLFNSYGPTE---ATVs 290
Cdd:PRK07788 288 LEDIAKHKATALVVVPVMLsrildlgpeVLAKYDTSSLKIIFVSGSALSPELATR-ALEAfgPVLYNLYGSTEvafATI- 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 291 ASLAELRPgDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELcIIGPGVA-EGYL-GRPELTAEKFLpnpwaehageprly 368
Cdd:PRK07788 366 ATPEDLAE-APGTVGRPPKGVTVKILDENGNEVPRGVVGRI-FVGNGFPfEGYTdGRDKQIIDGLL-------------- 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 369 RTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrqlpnGTAAVVLKPVDGID------QLVAYIVPGGGQPV 442
Cdd:PRK07788 430 SSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLA-----GHPDVVEAAVIGVDdeefgqRLRAFVVKAPGAAL 504
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 2772088563 443 QIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK07788 505 DEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELRE 547
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
26-485 |
2.03e-27 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 116.90 E-value: 2.03e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPfdadtpaervmvcledaqAVGIVTGH 105
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIP------------------ATTLLTPD 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 GLREGLDAIDCPIWDQPElMQKVDGPLLrrggagprhpayVIYTSGSTGKPKGV-------PINQGAICHF--LRSENER 176
Cdd:cd05974 63 DLRDRVDRGGAVYAAVDE-NTHADDPML------------LYFTSGTTSKPKLVehthrsyPVGHLSTMYWigLKPGDVH 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 177 LGVRADDRVYQGFSVAFdmsfeeiwISYLVGATLWIApREITADPDALPLALARQGITVLHAVPTLLALF------GADV 250
Cdd:cd05974 130 WNISSPGWAKHAWSCFF--------APWNAGATVFLF-NYARFDAKRVLAALVRYGVTTLCAPPTVWRMLiqqdlaSFDV 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 251 PsLRLINLGGEICPPALVER----WATPTRrlfNSYGPTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKllpAG 326
Cdd:cd05974 201 K-LREVVGAGEPLNPEVIEQvrraWGLTIR---DGYGQTETTALVGNSPGQPVKAGSMGRPLPGYRVALLDPDGA---PA 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 327 ATGELCI-IGP----GVAEGYLGRPELTAekflpnpwaeHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVEL 401
Cdd:cd05974 274 TEGEVALdLGDtrpvGLMKGYAGDPDKTA----------HAMRGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISP 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 402 GEIEAALSRQLPNGTAAVVLKPvDGIDQLV--AYIVPGGG---QPVQIAQLRAQLRETLPPYMVPSRFELVDtIPRLTSG 476
Cdd:cd05974 344 FELESVLIEHPAVAEAAVVPSP-DPVRLSVpkAFIVLRAGyepSPETALEIFRFSRERLAPYKRIRRLEFAE-LPKTISG 421
|
....*....
gi 2772088563 477 KIDRKALRA 485
Cdd:cd05974 422 KIRRVELRR 430
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
10-506 |
2.35e-27 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 118.34 E-value: 2.35e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPAEr 88
Cdd:PRK07786 27 ALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRlTPPE- 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 89 VMVCLEDAQAVGIVTGHGLREGLDAIDCpiwDQPELMQKV-------DGPL-----LRRGGAGPR-------HPAYVIYT 149
Cdd:PRK07786 106 IAFLVSDCGAHVVVTEAALAPVATAVRD---IVPLLSTVVvaggssdDSVLgyedlLAEAGPAHApvdipndSPALIMYT 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 150 SGSTGKPKGVPIN----QGAICHFLRsenerlGVRADDRVYQGF--SVAFDMS-FEEIWISYLVGATLWIAPREiTADPD 222
Cdd:PRK07786 183 SGTTGRPKGAVLThanlTGQAMTCLR------TNGADINSDVGFvgVPLFHIAgIGSMLPGLLLGAPTVIYPLG-AFDPG 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 223 ALPLALARQGITVLHAVPTLLALFGADVP------SLRLINLGGEICPPALVERWAT--PTRRLFNSYGPTEatVSASLA 294
Cdd:PRK07786 256 QLLDVLEAEKVTGIFLVPAQWQAVCAEQQarprdlALRVLSWGAAPASDTLLRQMAAtfPEAQILAAFGQTE--MSPVTC 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 295 ELRPGDPV----TIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlpnpwaeHAGeprLYRT 370
Cdd:PRK07786 334 MLLGEDAIrklgSVGKVIPTVAARVVDENMNDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAF-------AGG---WFHS 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 371 GDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVP-GGGQPVQIAQLR 448
Cdd:PRK07786 404 GDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVpVAVAAVrNDDAALTLEDLA 483
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 2772088563 449 AQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAwPMAALSATERSESDLPETEA 506
Cdd:PRK07786 484 EFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE-RYGACVNVERRSASAGFTER 540
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
5-483 |
4.37e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 117.40 E-value: 4.37e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADT 84
Cdd:PRK13383 40 LLAVTAARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEF 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAERVMVCLEDAQAVGIVTGHGLREGLDAIDcpiwdqpELMQKVDGPLL--RRGGAGPRHPA---YVIYTSGSTGKPKGV 159
Cdd:PRK13383 120 RSDALAAALRAHHISTVVADNEFAERIAGAD-------DAVAVIDPATAgaEESGGRPAVAApgrIVLLTSGTTGKPKGV 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 160 P----INQGAICHFLRSENERLgvRADDRVYQGFSVAFDMSFEEIWISYLVGATLwIAPREITADPDALPLALARQgiTV 235
Cdd:PRK13383 193 PrapqLRSAVGVWVTILDRTRL--RTGSRISVAMPMFHGLGLGMLMLTIALGGTV-LTHRHFDAEAALAQASLHRA--DA 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 236 LHAVPTLLALF---------GADVPSLRLINLGGEICPPALVERWA-TPTRRLFNSYGPTEATVS--ASLAELRPGdPVT 303
Cdd:PRK13383 268 FTAVPVVLARIlelpprvraRNPLPQLRVVMSSGDRLDPTLGQRFMdTYGDILYNGYGSTEVGIGalATPADLRDA-PET 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 304 IGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRpeltaekflpnpwAEHAGEPRLYRTGDLARIDEQGQIH 383
Cdd:PRK13383 347 VGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAGTRYTDG-------------GGKAVVDGMTSTGDMGYLDNAGRLF 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 384 CLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPS 462
Cdd:PRK13383 414 IVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHrLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPR 493
|
490 500
....*....|....*....|.
gi 2772088563 463 RFELVDTIPRLTSGKIDRKAL 483
Cdd:PRK13383 494 DINIVSSIPRNPTGKVLRKEL 514
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
2-461 |
4.94e-27 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 118.05 E-value: 4.94e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAwlpfd 81
Cdd:PRK08279 39 LGDVFEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAV----- 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 82 adtpaeRVMV-----------CLEDAQAVGIVTGHGLRE------GLDAIDCPIW-------DQPELMQKVDGPLLRRGG 137
Cdd:PRK08279 114 ------VALLntqqrgavlahSLNLVDAKHLIVGEELVEafeearADLARPPRLWvaggdtlDDPEGYEDLAAAAAGAPT 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 138 AGP--------RHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVY--------QGFSVAfdmsfeeiW 201
Cdd:PRK08279 188 TNPasrsgvtaKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYcclplyhnTGGTVA--------W 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 202 ISYLV-GATLWIApREITAD---PDAlplalARQGITVLHAVPTLLAlFGADVP--------SLRLI---NLGGEICpPA 266
Cdd:PRK08279 260 SSVLAaGATLALR-RKFSASrfwDDV-----RRYRATAFQYIGELCR-YLLNQPpkptdrdhRLRLMignGLRPDIW-DE 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 267 LVERWATPtrRLFNSYGPTEATVSASLAELRPGdpvTIGQ-PLP---NYGLLVVDAE-----------MKLLPAGATGEL 331
Cdd:PRK08279 332 FQQRFGIP--RILEFYAASEGNVGFINVFNFDG---TVGRvPLWlahPYAIVKYDVDtgepvrdadgrCIKVKPGEVGLL 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 332 C--IIGPGVAEGYlGRPELTAEKFLPNPWAEhaGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALS 409
Cdd:PRK08279 407 IgrITDRGPFDGY-TDPEASEKKILRDVFKK--GD-AWFNTGDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALS 482
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 410 RqlpngtaavvlkpVDGIDQLVAY---------------IVPGGGQPVQIAQLRAQLRETLPPYMVP 461
Cdd:PRK08279 483 G-------------FPGVEEAVVYgvevpgtdgragmaaIVLADGAEFDLAALAAHLYERLPAYAVP 536
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
9-493 |
6.10e-27 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 117.01 E-value: 6.10e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 9 TAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAwlPFDADTPAER 88
Cdd:PRK10946 32 TRHAASDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQR 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 89 VMVCLEDAQ---AVGIVT-GHGLREGLDAIDCPIWDQPELM----------QKVDGPLLRRGGAGPRHP------AYVIY 148
Cdd:PRK10946 110 SELNAYASQiepALLIADrQHALFSDDDFLNTLVAEHSSLRvvlllnddgeHSLDDAINHPAEDFTATPspadevAFFQL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 149 TSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVA--FDMSFEEIWISYLVGATLWIAPreitaDPDAL-- 224
Cdd:PRK10946 190 SGGSTGTPKLIPRTHNDYYYSVRRSVEICGFTPQTRYLCALPAAhnYPMSSPGALGVFLAGGTVVLAP-----DPSATlc 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 225 -PLaLARQGITVLHAVPTLLAL---------FGADVPSLRLINLGGEICPPALVERwaTPTR---RLFNSYGPTEATVSA 291
Cdd:PRK10946 265 fPL-IEKHQVNVTALVPPAVSLwlqaiaeggSRAQLASLKLLQVGGARLSETLARR--IPAElgcQLQQVFGMAEGLVNY 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 292 SlaelRPGDP-----VTIGQPL-PNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehagep 365
Cdd:PRK10946 342 T----RLDDSderifTTQGRPMsPDDEVWVADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGF------- 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 366 rlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvdgiDQLV-----AYIVPGggQ 440
Cdd:PRK10946 411 --YCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSME----DELMgekscAFLVVK--E 482
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 441 PVQIAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALSA 493
Cdd:PRK10946 483 PLKAVQLRRFLREQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA 536
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
15-503 |
1.85e-26 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 116.15 E-value: 1.85e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 15 DQTALVC----GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP-FDADTPaERV 89
Cdd:PRK04319 59 DKVALRYldasRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPlFEAFME-EAV 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 90 MVCLEDAQAVGIVTGHGLREGLDAIDCP-----------------IWDQPELMQKVDgPLLRRGGAGPRHPAYVIYTSGS 152
Cdd:PRK04319 138 RDRLEDSEAKVLITTPALLERKPADDLPslkhvllvgedveegpgTLDFNALMEQAS-DEFDIEWTDREDGAILHYTSGS 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGA-ICHFLrSENERLGVRADDrVY---------QGFSVAfdmsfeeIWISYLVGATLWIAPREItaDPD 222
Cdd:PRK04319 217 TGKPKGVLHVHNAmLQHYQ-TGKYVLDLHEDD-VYwctadpgwvTGTSYG-------IFAPWLNGATNVIDGGRF--SPE 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 223 ALPLALARQGITVLHAVPTLL-ALFGA--------DVPSLRLINLGGEICPPALVeRWATPT--RRLFNSYGPTE--ATV 289
Cdd:PRK04319 286 RWYRILEDYKVTVWYTAPTAIrMLMGAgddlvkkyDLSSLRHILSVGEPLNPEVV-RWGMKVfgLPIHDNWWMTEtgGIM 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 290 SASLA--ELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIG--PGVAEGYLGRPELTAEKFLPNpWaehagep 365
Cdd:PRK04319 365 IANYPamDIKPG---SMGKPLPGIEAAIVDDQGNELPPNRMGNLAIKKgwPSMMRGIWNNPEKYESYFAGD-W------- 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 366 rlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvdgiDQLVAYIVPG------GG 439
Cdd:PRK04319 434 --YVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKP----DPVRGEIIKAfvalrpGY 507
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2772088563 440 QPVQ--IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWpmaalsatersESDLPE 503
Cdd:PRK04319 508 EPSEelKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKAW-----------ELGLPE 562
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
26-485 |
2.48e-26 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 116.01 E-value: 2.48e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA----WLPFDADTPAERVmvclEDAQAVGI 101
Cdd:PRK00174 99 ITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVhsvvFGGFSAEALADRI----IDAGAKLV 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 102 VTG-HGLREG----L-DAIDCPIwDQPELMQKVdgPLLRRGGA----------------------------GPRHPAYVI 147
Cdd:PRK00174 175 ITAdEGVRGGkpipLkANVDEAL-ANCPSVEKV--IVVRRTGGdvdwvegrdlwwhelvagasdecepepmDAEDPLFIL 251
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 148 YTSGSTGKPKGVPINQGAichFLrsenerLGVraddrvYQGFSVAFDMSFEEI--------WI---SYLV------GATL 210
Cdd:PRK00174 252 YTSGSTGKPKGVLHTTGG---YL------VYA------AMTMKYVFDYKDGDVywctadvgWVtghSYIVygplanGATT 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 211 WI---APReiTADPDALPLALARQGITVLHAVPTLLALF---GADVP------SLRL-------IN----------LGGE 261
Cdd:PRK00174 317 LMfegVPN--YPDPGRFWEVIDKHKVTIFYTAPTAIRALmkeGDEHPkkydlsSLRLlgsvgepINpeawewyykvVGGE 394
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 262 ICPpaLVERW---------------ATPtrrlfnsygpteatvsaslaeLRPGdpvTIGQPLPNYGLLVVDAEMKLLPAG 326
Cdd:PRK00174 395 RCP--IVDTWwqtetggimitplpgATP---------------------LKPG---SATRPLPGIQPAVVDEEGNPLEGG 448
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 327 ATGELCIIG--PGVAEGYLGRPEltaeKFLPNPWAEHAGEprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEI 404
Cdd:PRK00174 449 EGGNLVIKDpwPGMMRTIYGDHE----RFVKTYFSTFKGM---YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEI 521
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 405 EAALSRQLPNGTAAVVLKPVD----GIdqlVAYIVPGGGQPVQ---IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGK 477
Cdd:PRK00174 522 ESALVAHPKVAEAAVVGRPDDikgqGI---YAFVTLKGGEEPSdelRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGK 598
|
....*...
gi 2772088563 478 IDRKALRA 485
Cdd:PRK00174 599 IMRRILRK 606
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
249-484 |
7.26e-26 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 114.15 E-value: 7.26e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 DVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSAS------LAELRpgdpvTIGQPLPNYGLLVVDAEMK 321
Cdd:PRK12492 331 DFSALKLTNSGGTALVKATAERWEQLTGcTIVEGYGLTETSPVAStnpygeLARLG-----TVGIPVPGTALKVIDDDGN 405
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 322 LLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVEL 401
Cdd:PRK12492 406 ELPLGERGELCIKGPQVMKGYWQQPEATAEALDAEGW---------FKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYP 476
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 402 GEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRK 481
Cdd:PRK12492 477 NEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRR 556
|
...
gi 2772088563 482 ALR 484
Cdd:PRK12492 557 ELR 559
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
9-485 |
9.12e-26 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 112.47 E-value: 9.12e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 9 TAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTP--- 85
Cdd:cd05929 1 LEARDLDRAQVFHQRRLLLLDVYSIALNRNARAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPrae 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 86 AERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPElmqkvdgPLLRRGGAGprhpAYVIYTSGSTGKPKGVPINQGa 165
Cdd:cd05929 81 ACAIIEIKAAALVCGLFTGGGALDGLEDYEAAEGGSPE-------TPIEDEAAG----WKMLYSGGTTGRPKGIKRGLP- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 166 ichflrsenerlGVRADDRVYQGFSVAFDMSFEEIWIS----------------YLVGATLWIAPReitADPDALPLALA 229
Cdd:cd05929 149 ------------GGPPDNDTLMAAALGFGPGADSVYLSpaplyhaapfrwsmtaLFMGGTLVLMEK---FDPEEFLRLIE 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 230 RQGITVLHAVPT----LLALFGA-----DVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEA---TVSASLAEL 296
Cdd:cd05929 214 RYRVTFAQFVPTmfvrLLKLPEAvrnayDLSSLKRVIHAAAPCPPWVKEQWiDWGGPIIWEYYGGTEGqglTIINGEEWL 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 297 R-PGdpvTIGQPLPNyGLLVVDAEMKLLPAGATGELCIIGPGVAEgYLGRPELTAEKFLPNPWAEhageprlyrTGDLAR 375
Cdd:cd05929 294 ThPG---SVGRAVLG-KVHILDEDGNEVPPGEIGEVYFANGPGFE-YTNDPEKTAAARNEGGWST---------LGDVGY 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIV---PGGGQPVQIAQ-LRAQL 451
Cdd:cd05929 360 LDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVqpaPGADAGTALAEeLIAFL 439
|
490 500 510
....*....|....*....|....*....|....
gi 2772088563 452 RETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05929 440 RDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLRD 473
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
142-480 |
9.58e-26 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 109.42 E-value: 9.58e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIaprEITADP 221
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIG---QRKFNP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLALARQGITVLHAVPTLL-ALFGADVP--SLRLINLGGEICPPALVE--RWATPTRRLFNSYGPTEATVSASLA-- 294
Cdd:cd17633 78 KSWIRKINQYNATVIYLVPTMLqALARTLEPesKIKSIFSSGQKLFESTKKklKNIFPKANLIEFYGTSELSFITYNFnq 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 295 ELRPgdPVTIGQPLPNygllvVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKflpnpWaehageprlYRTGDLA 374
Cdd:cd17633 158 ESRP--PNSVGRPFPN-----VEIEIRNADGGEIGKIFVKSEMVFSGYVRGGFSNPDG-----W---------MSVGDIG 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 375 RIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLK-PVDGIDQLVAYIVPGGGqpVQIAQLRAQLRE 453
Cdd:cd17633 217 YVDEEGYLYLVGRESDMIIIGGINIFPTEIESVL-KAIPGIEEAIVVGiPDARFGEIAVALYSGDK--LTYKQLKRFLKQ 293
|
330 340
....*....|....*....|....*..
gi 2772088563 454 TLPPYMVPSRFELVDTIPRLTSGKIDR 480
Cdd:cd17633 294 KLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
2-393 |
1.36e-25 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 113.07 E-value: 1.36e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGAL----------SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIA 71
Cdd:PRK09274 8 IARHLPRAAQERPDQLAVAVPGGrgadgklaydELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALF 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 72 KTGAAWLPFDADTPAERVMVCLEDAQA---VGIVTGHGLREGLDaidcpiWDQPELMQK--VDGPL---------LRRGG 137
Cdd:PRK09274 88 KAGAVPVLVDPGMGIKNLKQCLAEAQPdafIGIPKAHLARRLFG------WGKPSVRRLvtVGGRLlwggttlatLLRDG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 138 AG---------PRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVafdMSFeeiwISYLVGA 208
Cdd:PRK09274 162 AAapfpmadlaPDDMAAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPL---FAL----FGPALGM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 209 TLWIAP----REITADPDALPLALARQGITVLHAVPTLLALFGAD-------VPSLRLINLGGEICPPALVER---WATP 274
Cdd:PRK09274 235 TSVIPDmdptRPATVDPAKLFAAIERYGVTNLFGSPALLERLGRYgeangikLPSLRRVISAGAPVPIAVIERfraMLPP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 275 TRRLFNSYGPTEA------TVSASLAELR----PGDPVTIGQPLPNYGLLVVD---------AEMKLLPAGATGELCIIG 335
Cdd:PRK09274 315 DAEILTPYGATEAlpissiESREILFATRaatdNGAGICVGRPVDGVEVRIIAisdapipewDDALRLATGEIGEIVVAG 394
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 2772088563 336 PGVAEGYLGRPELTAEKFLPNPWAEHageprLYRTGDLARIDEQGQIHCLGRADDQVK 393
Cdd:PRK09274 395 PMVTRSYYNRPEATRLAKIPDGQGDV-----WHRMGDLGYLDAQGRLWFCGRKAHRVE 447
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
2-484 |
5.11e-25 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 111.30 E-value: 5.11e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPrgiDLLasQLAIAKTG---AAW 77
Cdd:PRK08974 25 LVDMFEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMP---NLL--QYPIALFGilrAGM 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 78 LPFDAD---TPAErvmvcLE----DAQAVGIVT----GHGLREGLD------AIDCPIWDQ----------------PEL 124
Cdd:PRK08974 100 IVVNVNplyTPRE-----LEhqlnDSGAKAIVIvsnfAHTLEKVVFktpvkhVILTRMGDQlstakgtlvnfvvkyiKRL 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 125 MQKVDGP-------LLRRGgagpRHPAYVI------------YTSGSTGKPKGVPINQG-----------AICHFLRsEN 174
Cdd:PRK08974 175 VPKYHLPdaisfrsALHKG----RRMQYVKpelvpedlaflqYTGGTTGVAKGAMLTHRnmlanleqakaAYGPLLH-PG 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 175 ERLGVRADDrVYQGFSVAFD-MSFEEIWISYLvgatLWIAPREItadpDALPLALARQGITVLHAVPTLlalFGA----- 248
Cdd:PRK08974 250 KELVVTALP-LYHIFALTVNcLLFIELGGQNL----LITNPRDI----PGFVKELKKYPFTAITGVNTL---FNAllnne 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 -----DVPSLRLINLGGEICPPALVERWATPT-RRLFNSYGPTEAtvsASLAELRPGDPV----TIGQPLPNYGLLVVDA 318
Cdd:PRK08974 318 efqelDFSSLKLSVGGGMAVQQAVAERWVKLTgQYLLEGYGLTEC---SPLVSVNPYDLDyysgSIGLPVPSTEIKLVDD 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 319 EMKLLPAGATGELCIIGPGVAEGYLGRPELTAEkFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFR 398
Cdd:PRK08974 395 DGNEVPPGEPGELWVKGPQVMLGYWQRPEATDE-VIKDGW---------LATGDIAVMDEEGFLRIVDRKKDMILVSGFN 464
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 399 VELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:PRK08974 465 VYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKI 544
|
....*.
gi 2772088563 479 DRKALR 484
Cdd:PRK08974 545 LRRELR 550
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
3-485 |
6.33e-25 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 111.00 E-value: 6.33e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 3 ADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDA 82
Cdd:PRK13382 46 TSGFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNT 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 83 DTPAERVMVCLEDAQAVGIVTGHGLREGLDAI--DCP------IW----DQPELMQKVDGPLLRRGGAGPRHPAYVIYTS 150
Cdd:PRK13382 126 SFAGPALAEVVTREGVDTVIYDEEFSATVDRAlaDCPqatrivAWtdedHDLTVEVLIAAHAGQRPEPTGRKGRVILLTS 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 151 GSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLwIAPREItaDPDALPLALAR 230
Cdd:PRK13382 206 GTTGTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFHAWGFSQLVLAASLACTI-VTRRRF--DPEATLDLIDR 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 231 QGITVLHAVPTLLALFgADVP----------SLRLINLGGEICPPALVErwATPTR---RLFNSYGPTEATV--SASLAE 295
Cdd:PRK13382 283 HRATGLAVVPVMFDRI-MDLPaevrnrysgrSLRFAAASGSRMRPDVVI--AFMDQfgdVIYNNYNATEAGMiaTATPAD 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 296 LRPGdPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRpelTAEKFlpnpwaeHAGeprLYRTGDLAR 375
Cdd:PRK13382 360 LRAA-PDTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGYTSG---STKDF-------HDG---FMASGDVGY 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVPGGGQPVQIAQLRAQLRET 454
Cdd:PRK13382 426 LDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQrLAAFVVLKPGASATPETLKQHVRDN 505
|
490 500 510
....*....|....*....|....*....|.
gi 2772088563 455 LPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK13382 506 LANYKVPRDIVVLDELPRGATGKILRRELQA 536
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
2-603 |
8.07e-25 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 113.34 E-value: 8.07e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVC------GALSLSYRELDERASLMAHHLIKQGVrPGQIVGLWLPRGIDLLASQLAIAKTGA 75
Cdd:PRK05691 11 LVQALQRRAAQTPDRLALRFladdpgEGVVLSYRDLDLRARTIAAALQARAS-FGDRAVLLFPSGPDYVAAFFGCLYAGV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 76 AWLPfdADTPA-------ERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQ-PELMQkVDG--PLLRRGGAGPR---- 141
Cdd:PRK05691 90 IAVP--AYPPEsarrhhqERLLSIIADAEPRLLLTVADLRDSLLQMEELAAANaPELLC-VDTldPALAEAWQEPAlqpd 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HPAYVIYTSGSTGKPKGVPINQGAIC--HFLRSENERLGVRADDRVYQGFSVAFDMSfeeiwisyLVGATL--------- 210
Cdd:PRK05691 167 DIAFLQYTSGSTALPKGVQVSHGNLVanEQLIRHGFGIDLNPDDVIVSWLPLYHDMG--------LIGGLLqpifsgvpc 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 211 -WIAPREITADPDALPLALARQGITV----------LHAVPTLLALFGADVPSLRLINLGGEICPPALVERWA------- 272
Cdd:PRK05691 239 vLMSPAYFLERPLRWLEAISEYGGTIsggpdfayrlCSERVSESALERLDLSRWRVAYSGSEPIRQDSLERFAekfaacg 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 273 -TPTRrLFNSYGPTEATV---------------------SASLAELRPGDPV-TIGQPLPNYGLLVVD-AEMKLLPAGAT 328
Cdd:PRK05691 319 fDPDS-FFASYGLAEATLfvsggrrgqgipaleldaealARNRAEPGTGSVLmSCGRSQPGHAVLIVDpQSLEVLGDNRV 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 329 GELCIIGPGVAEGYLGRPELTAEKFLpnpwaEHAGEPRLyRTGDLARIDEqGQIHCLGRADDQVKVRGFRVELGEIEAAL 408
Cdd:PRK05691 398 GEIWASGPSIAHGYWRNPEASAKTFV-----EHDGRTWL-RTGDLGFLRD-GELFVTGRLKDMLIVRGHNLYPQDIEKTV 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 409 SRQLP---NGTAAVVLKPVDG---------IDQLVAYIVPGggqPVQIAQLRAQLRETLppYMVPSRFELVD--TIPRLT 474
Cdd:PRK05691 471 EREVEvvrKGRVAAFAVNHQGeegigiaaeISRSVQKILPP---QALIKSIRQAVAEAC--QEAPSVVLLLNpgALPKTS 545
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 475 SGKIDRKALRAW-------PMAALSATERSESDLPETEAE--TALFAALARFFPGLPIQRGGDFFDDLGGHSLLAARLVS 545
Cdd:PRK05691 546 SGKLQRSACRLRladgsldSYALFPALQAVEAAQTAASGDelQARIAAIWCEQLKVEQVAADDHFFLLGGNSIAATQVVA 625
|
650 660 670 680 690 700
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 546 ALREDgryAGMTVN--DVYGQRRVGAIAAAMEAGQARVAAEPMVFATVPRPRRWLCGLAQ 603
Cdd:PRK05691 626 RLRDE---LGIDLNlrQLFEAPTLAAFSAAVARQLAGGGAAQAAIARLPRGQALPQSLAQ 682
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
249-484 |
1.51e-24 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 109.85 E-value: 1.51e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 DVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGA 327
Cdd:PRK05677 324 DFSALKLTLSGGMALQLATAERWKEVTGcAICEGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVIDDDGNELPLGE 403
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 328 TGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAA 407
Cdd:PRK05677 404 VGELCVKGPQVMKGYWQRPEATDEILDSDGW---------LKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDV 474
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 408 LSrQLPN--GTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK05677 475 LA-ALPGvlQCAAIGVPDEKSGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
2-483 |
1.86e-24 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 109.59 E-value: 1.86e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGA--LSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP 79
Cdd:PRK05852 18 IADLVEVAATRLPEAPALVVTAdrIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVP 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 80 FDADTPAERVMVCLEDAQA-VGIVTGHGLREGlDAIDCPIWDQPELMQKVDGP-----LLRRGGAGPRHP---------- 143
Cdd:PRK05852 98 LDPALPIAEQRVRSQAAGArVVLIDADGPHDR-AEPTTRWWPLTVNVGGDSGPsggtlSVHLDAATEPTPatstpeglrp 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 --AYVIYTSGSTGKPKGVPINQGAICHFLRS--ENERLGVRaDDRV-----YQGFSVAFDMsfeeiwISYLVGATLWIAP 214
Cdd:PRK05852 177 ddAMIMFTGGTTGLPKMVPWTHANIASSVRAiiTGYRLSPR-DATVavmplYHGHGLIAAL------LATLASGGAVLLP 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 215 REITADPDALPLALARQGITVLHAVPT----LLAL-----FGADVPSLRLInlggEICPPALVERWATPTRRLFNS---- 281
Cdd:PRK05852 250 ARGRFSAHTFWDDIKAVGATWYTAVPTihqiLLERaatepSGRKPAALRFI----RSCSAPLTAETAQALQTEFAApvvc 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 282 -YGPTEATVSASLAEL----RPGDPVTIGQPLPNYG---LLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKF 353
Cdd:PRK05852 326 aFGMTEATHQVTTTQIegigQTENPVVSTGLVGRSTgaqIRIVGSDGLPLPAGAVGEVWLRGTTVVRGYLGDPTITAANF 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 354 lPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQlPNGTAAVVLKPVDGI--DQLV 431
Cdd:PRK05852 406 -TDGW---------LRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASH-PNVMEAAVFGVPDQLygEAVA 474
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 432 AYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:PRK05852 475 AVIVPRESAPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAV 526
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
4-489 |
8.35e-24 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 108.12 E-value: 8.35e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVC--------GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGID----LLASQLA-I 70
Cdd:PRK07529 29 ELLSRAAARHPDAPALSFlldadpldRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPEthfaLWGGEAAgI 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 71 A-----------------KTGAAWL----PF-DADT-----------PAERVMVCLEDAQAVGIVTGHGLREGLDAIDCP 117
Cdd:PRK07529 109 AnpinpllepeqiaellrAAGAKVLvtlgPFpGTDIwqkvaevlaalPELRTVVEVDLARYLPGPKRLAVPLIRRKAHAR 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 118 IWDQPELMQKVDGPLLRRGGA-GPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQG---FSVaf 193
Cdd:PRK07529 189 ILDFDAELARQPGDRLFSGRPiGPDDVAAYFHTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGlplFHV-- 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 194 DMSFEEIWISYLVGATLWIAPREITADPDALP---LALARQGITVLHAVPTLLALF------GADVPSLRLINLGGEICP 264
Cdd:PRK07529 267 NALLVTGLAPLARGAHVVLATPQGYRGPGVIAnfwKIVERYRINFLSGVPTVYAALlqvpvdGHDISSLRYALCGAAPLP 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 265 PALVERWATPTR-RLFNSYGPTEATVSASLA----ELRPGdpvTIGQPLP--NYGLLVVDAEMKLL---PAGATGELCII 334
Cdd:PRK07529 347 VEVFRRFEAATGvRIVEGYGLTEATCVSSVNppdgERRIG---SVGLRLPyqRVRVVILDDAGRYLrdcAVDEVGVLCIA 423
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 335 GPGVAEGYLgrpelTAEKFLpNPWAEhagePRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPN 414
Cdd:PRK07529 424 GPNVFSGYL-----EAAHNK-GLWLE----DGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAV 493
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 415 GTAAVVLKPVDGIDQL-VAYI--VPGGGqpVQIAQLRAQLRETLP-PYMVPSRFELVDTIPRLTSGKIDRKALRAWPMA 489
Cdd:PRK07529 494 ALAAAVGRPDAHAGELpVAYVqlKPGAS--ATEAELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPALRRDAIR 570
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
249-489 |
1.40e-23 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 106.88 E-value: 1.40e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 DVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSASLAELRPGD-PVTIGQPLPNYGLLVVDAEMKLLPAG 326
Cdd:PRK08751 327 DFSSLKMTLGGGMAVQRSVAERWKQVTGlTLVEAYGLTETSPAACINPLTLKEyNGSIGLPIPSTDACIKDDAGTVLAIG 406
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 327 ATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEA 406
Cdd:PRK08751 407 EIGELCIKGPQVMKGYWKRPEETAKVMDADGW---------LHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIED 477
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 407 ALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK08751 478 VIAMMPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDA 557
|
...
gi 2772088563 487 PMA 489
Cdd:PRK08751 558 AKA 560
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
26-436 |
1.86e-23 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 105.52 E-value: 1.86e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVtgh 105
Cdd:cd17640 6 ITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALV--- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 glregldaidcpiwdqpelmqkVDGpllrrggaGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRV 185
Cdd:cd17640 83 ----------------------VEN--------DSDDLATIIYTSGTTGNPKGVMLTHANLLHQIRSLSDIVPPQPGDRF 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 186 yqgfsvafdMSFEEIWISYLVGATLWIAPR---EITADPDALPLALARQGITVLHAVPTLL------------------- 243
Cdd:cd17640 133 ---------LSILPIWHSYERSAEYFIFACgcsQAYTSIRTLKDDLKRVKPHYIVSVPRLWeslysgiqkqvsksspikq 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALFGADVPSLRL---INLGGEIcPPALVERWATPTRRLFNSYGPTEatVSASLAELRPGDPV--TIGQPLPNYGLLVVDA 318
Cdd:cd17640 204 FLFLFFLSGGIFkfgISGGGAL-PPHVDTFFEAIGIEVLNGYGLTE--TSPVVSARRLKCNVrgSVGRPLPGTEIKIVDP 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 319 EMK-LLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVR-G 396
Cdd:cd17640 281 EGNvVLPPGEKGIVWVRGPQVMKGYYKNPEATSKVLDSDGW---------FNTGDLGWLTCGGELVLTGRAKDTIVLSnG 351
|
410 420 430 440
....*....|....*....|....*....|....*....|..
gi 2772088563 397 FRVELGEIEAALSRQLPNGTAAVVlkpvdGIDQ--LVAYIVP 436
Cdd:cd17640 352 ENVEPQPIEEALMRSPFIEQIMVV-----GQDQkrLGALIVP 388
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
141-480 |
3.01e-23 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 102.72 E-value: 3.01e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 141 RHPAYVIYTSGSTGKPKGVPINQGAIchFLRSEN---ERLGVRADDRVYQGFSVAFdmSFEEIWISYLVGATLWIAPREI 217
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTF--FAVPDIlqkEGLNWVVGDVTYLPLPATH--IGGLWWILTCLIHGGLCVTGGE 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 TADPDALPLALARQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEATV 289
Cdd:cd17635 77 NTTYKSLFKILTTNAVTTTCLVPTLLSKLvselksaNATVPSLRLIGYGGSRAIAADVRFIeATGLTNTAQVYGLSETGT 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 290 SASLAELR-PGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWaehageprlY 368
Cdd:cd17635 157 ALCLPTDDdSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPANMLGYWNNPERTAEVLI-DGW---------V 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 369 RTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLKPVDGI--DQLVAYIVPGGGQ-PVQIA 445
Cdd:cd17635 227 NTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAE-GVSGVQECACYEISDEEfgELVGLAVVASAELdENAIR 305
|
330 340 350
....*....|....*....|....*....|....*
gi 2772088563 446 QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDR 480
Cdd:cd17635 306 ALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
10-485 |
3.71e-23 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 105.45 E-value: 3.71e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGAL--SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWL---PFDadT 84
Cdd:PLN02246 33 LSEFSDRPCLIDGATgrVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTtanPFY--T 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 85 PAE--------RVMVCLEDAQAVGIVTGHGLREGLD--AIDCP------IWdqpELMQKVDGPLlRRGGAGPRHPAYVIY 148
Cdd:PLN02246 111 PAEiakqakasGAKLIITQSCYVDKLKGLAEDDGVTvvTIDDPpegclhFS---ELTQADENEL-PEVEISPDDVVALPY 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 149 TSGSTGKPKGVPINQG----AICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYL-VGATLWIAPR-EITAdpd 222
Cdd:PLN02246 187 SSGTTGLPKGVMLTHKglvtSVAQQVDGENPNLYFHSDDVILCVLPMFHIYSLNSVLLCGLrVGAAILIMPKfEIGA--- 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 223 aLPLALARQGITVLHAVPTL-LALFGA------DVPSLRLI-----NLGGEIcPPALVERWatPTRRLFNSYGPTEA--T 288
Cdd:PLN02246 264 -LLELIQRHKVTIAPFVPPIvLAIAKSpvvekyDLSSIRMVlsgaaPLGKEL-EDAFRAKL--PNAVLGQGYGMTEAgpV 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 VSASLAELRPGDPVTIGqplpNYGLLVVDAEMKL--------LPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAe 360
Cdd:PLN02246 340 LAMCLAFAKEPFPVKSG----SCGTVVRNAELKIvdpetgasLPRNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWL- 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 361 HageprlyrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGG 439
Cdd:PLN02246 415 H--------TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVpVAFVVRSNG 486
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2772088563 440 QPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PLN02246 487 SEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLRA 532
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
228-485 |
5.84e-23 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 102.17 E-value: 5.84e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGITVLHAVPTLLALF-----GADVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSASLA----ELR 297
Cdd:cd05944 93 VERYRITSLSTVPTVYAALlqvpvNADISSLRFAMSGAAPLPVELRARFEDATGlPVVEGYGLTEATCLVAVNppdgPKR 172
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGdpvTIGQPLPNYG--LLVVDAEMKLL---PAGATGELCIIGPGVAEGYLgRPELTAEKFlpnpwaehaGEPRLYRTGD 372
Cdd:cd05944 173 PG---SVGLRLPYARvrIKVLDGVGRLLrdcAPDEVGEICVAGPGVFGGYL-YTEGNKNAF---------VADGWLNTGD 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 373 LARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQL 451
Cdd:cd05944 240 LGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELpVAYVQLKPGAVVEEEELLAWA 319
|
250 260 270
....*....|....*....|....*....|....*
gi 2772088563 452 RETLPPY-MVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:cd05944 320 RDHVPERaAVPKHIEVLEELPVTAVGKVFKPALRA 354
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
145-479 |
1.17e-22 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 101.30 E-value: 1.17e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 145 YVIYTSGSTGKPKGVPINQGAI-------CHFLRSENERLGVRADDRVYQGFSVAFD----MSFEEIWISY--LVGATLW 211
Cdd:cd05924 7 YILYTGGTTGMPKGVMWRQEDIfrmlmggADFGTGEFTPSEDAHKAAAAAAGTVMFPapplMHGTGSWTAFggLLGGQTV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 212 IAPREiTADPDALPLALARQGITVLHAV------PTLLALFGA---DVPSLRLINLGGEICPPALVERW--ATPTRRLFN 280
Cdd:cd05924 87 VLPDD-RFDPEEVWRTIEKHKVTSMTIVgdamarPLIDALRDAgpyDLSSLFAISSGGALLSPEVKQGLleLVPNITLVD 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 281 SYGPTEATVSASlAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPG-VAEGYLGRPELTAEKFlpnpwa 359
Cdd:cd05924 166 AFGSSETGFTGS-GHSAGSGPETGPFTRANPDTVVLDDDGRVVPPGSGGVGWIARRGhIPLGYYGDEAKTAETF------ 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 360 EHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQlPNGTAAVVL-KPVDGIDQLVAYIV--- 435
Cdd:cd05924 239 PEVDGVRYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSH-PAVYDVLVVgRPDERWGQEVVAVVqlr 317
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 2772088563 436 PGGGqpVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKID 479
Cdd:cd05924 318 EGAG--VDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
25-484 |
4.28e-22 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 101.90 E-value: 4.28e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTG 104
Cdd:PRK08276 11 VVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVS 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 HGLREGLDAIDCPIWDQPELMQKVDGPLlrrGGAGP------RHPAYVI----------YTSGSTGKPKGV--PINQGAI 166
Cdd:PRK08276 91 AALADTAAELAAELPAGVPLLLVVAGPV---PGFRSyeealaAQPDTPIadetagadmlYSSGTTGRPKGIkrPLPGLDP 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 167 chflrseNERLGVRAddrvyqgFSVAFDMSFEEIWIsYLVGATLW-IAPREITA---------------DPDALPLALAR 230
Cdd:PRK08276 168 -------DEAPGMML-------ALLGFGMYGGPDSV-YLSPAPLYhTAPLRFGMsalalggtvvvmekfDAEEALALIER 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 231 QGITVLHAVPT----LLALFGA-----DVPSLRLINLGGEICPPALVER---WATPTrrLFNSYGPTEA---TVSASLAE 295
Cdd:PRK08276 233 YRVTHSQLVPTmfvrMLKLPEEvraryDVSSLRVAIHAAAPCPVEVKRAmidWWGPI--IHEYYASSEGggvTVITSEDW 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 296 L-RPGdpvTIGQPLPNyGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEhageprlyrTGDLA 374
Cdd:PRK08276 311 LaHPG---SVGKAVLG-EVRILDEDGNELPPGEIGTVYFEMDGYPFEYHNDPEKTAAARNPHGWVT---------VGDVG 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 375 RIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGI-DQLVAYIVPGGG---QPVQIAQLRAQ 450
Cdd:PRK08276 378 YLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMgERVKAVVQPADGadaGDALAAELIAW 457
|
490 500 510
....*....|....*....|....*....|....
gi 2772088563 451 LRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK08276 458 LRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
144-483 |
1.44e-21 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 100.49 E-value: 1.44e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 AYVIYTSGSTGKPKGVPINQGAIC-------HFL----RSENERLGVRADDRVYqGFSVAFDMSFEEiwisylvGATLWI 212
Cdd:PRK06710 209 ALLQYTGGTTGFPKGVMLTHKNLVsntlmgvQWLynckEGEEVVLGVLPFFHVY-GMTAVMNLSIMQ-------GYKMVL 280
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 213 APReitADPDALPLALARQGITVLHAVPTL-LALFGA------DVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGP 284
Cdd:PRK06710 281 IPK---FDMKMVFEAIKKHKVTLFPGAPTIyIALLNSpllkeyDISSIRACISGSAPLPVEVQEKFETVTGgKLVEGYGL 357
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 285 TEA---TVSASLAELRPgdPVTIGQPLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGRPELTAeKFLPNPWae 360
Cdd:PRK06710 358 TESspvTHSNFLWEKRV--PGSIGVPWPDTEAMIMSLETgEALPPGEIGEIVVKGPQIMKGYWNKPEETA-AVLQDGW-- 432
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 361 hageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV--VLKPVDGiDQLVAYIVPGG 438
Cdd:PRK06710 433 -------LHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTigVPDPYRG-ETVKAFVVLKE 504
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 2772088563 439 GQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:PRK06710 505 GTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
24-455 |
2.24e-21 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 99.47 E-value: 2.24e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 24 LSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVT 103
Cdd:cd05932 5 VEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 104 G---------HGLREGLDAIDCPIWDQP------ELMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICH 168
Cdd:cd05932 85 GklddwkamaPGVPEGLISISLPPPSAAncqyqwDDLIAQHPPLEERPTRFPEQLATLIYTSGTTGQPKGVMLTFGSFAW 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 169 FLRSENERLGVRADDRVYQGFSVAFdmSFEEIWI--SYLVGATLWIAPREItadpDALPLALARQGITVLHAVPTLLALF 246
Cdd:cd05932 165 AAQAGIEHIGTEENDRMLSYLPLAH--VTERVFVegGSLYGGVLVAFAESL----DTFVEDVQRARPTLFFSVPRLWTKF 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 --------------------------------GADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLA 294
Cdd:cd05932 239 qqgvqdkipqqklnlllkipvvnslvkrkvlkGLGLDQCRLAGCGSAPVPPALLEWYRSLGLNILEAYGMTENFAYSHLN 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 295 elRPGDPV--TIGQPLPnygllvvDAEMKLlpaGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGD 372
Cdd:cd05932 319 --YPGRDKigTVGNAGP-------GVEVRI---SEDGEILVRSPALMMGYYKDPEATAEAFTADGF---------LRTGD 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 373 LARIDEQGQIHCLGRADDQVKV-RGFRVELGEIEAALSrQLPNGTAAVVLKpvDGIDQLVAYIVPG-GGQPVQIAQLRAQ 450
Cdd:cd05932 378 KGELDADGNLTITGRVKDIFKTsKGKYVAPAPIENKLA-EHDRVEMVCVIG--SGLPAPLALVVLSeEARLRADAFARAE 454
|
....*
gi 2772088563 451 LRETL 455
Cdd:cd05932 455 LEASL 459
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
10-484 |
2.88e-21 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 99.38 E-value: 2.88e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGAL--SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPA 86
Cdd:PRK13391 7 AQTTPDKPAVIMASTgeVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHlTPA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ERVMVcLEDAQAVGIVTGHGLRE--GLDAIDCPIWdQPELMQKVDGPLLRRGG---AGPRHPAYVI----------YTSG 151
Cdd:PRK13391 87 EAAYI-VDDSGARALITSAAKLDvaRALLKQCPGV-RHRLVLDGDGELEGFVGyaeAVAGLPATPIadeslgtdmlYSSG 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 152 STGKPKGV--------PINQGAICHFLrseNERLGVRADDR------VYQGFSVAFDMSFEEiwisylVGATLWIAPRei 217
Cdd:PRK13391 165 TTGRPKGIkrplpeqpPDTPLPLTAFL---QRLWGFRSDMVylspapLYHSAPQRAVMLVIR------LGGTVIVMEH-- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 tADPDALPLALARQGITVLHAVPTL----LALFGA-----DVPSLRLINLGGEICPPALVE---RWATPTrrLFNSYGPT 285
Cdd:PRK13391 234 -FDAEQYLALIEEYGVTHTQLVPTMfsrmLKLPEEvrdkyDLSSLEVAIHAAAPCPPQVKEqmiDWWGPI--IHEYYAAT 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 286 EAT-VSASLAELRPGDPVTIGQPLpnYGLLVV-DAEMKLLPAGATGELCIIGpGVAEGYLGRPELTAEkflpnpwAEHAg 363
Cdd:PRK13391 311 EGLgFTACDSEEWLAHPGTVGRAM--FGDLHIlDDDGAELPPGEPGTIWFEG-GRPFEYLNDPAKTAE-------ARHP- 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 364 EPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIV---PGGGQ 440
Cdd:PRK13391 380 DGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVqpvDGVDP 459
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 2772088563 441 PVQIAQ-LRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK13391 460 GPALAAeLIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
146-480 |
5.45e-21 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 95.80 E-value: 5.45e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 VIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQ--------GFSVAFDMsfeeiwisYLVGATLWIAPRei 217
Cdd:cd17637 5 IIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNmlplfhiaGLNLALAT--------FHAGGANVVMEK-- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 tADPDALPLALARQGITVLHAVPTLLALF-------GADVPSLRLInLGGEIcpPALVERWATPTRRLFNS-YGPTEAT- 288
Cdd:cd17637 75 -FDPAEALELIEEEKVTLMGSFPPILSNLldaaeksGVDLSSLRHV-LGLDA--PETIQRFEETTGATFWSlYGQTETSg 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 -VSASLAELRPGdpvTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprl 367
Cdd:cd17637 151 lVTLSPYRERPG---SAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRGPLVFQGYWNLPELTAYTF-RNGW--------- 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 368 YRTGDLARIDEQGQIHCLGR--ADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLKPVD-----GIDqlvAYIVPGGGQ 440
Cdd:cd17637 218 HHTGDLGRFDEDGYLWYAGRkpEKELIKPGGENVYPAEVEKVI-LEHPAIAEVCVIGVPDpkwgeGIK---AVCVLKPGA 293
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 2772088563 441 PVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDR 480
Cdd:cd17637 294 TLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
114-485 |
2.79e-20 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 96.29 E-value: 2.79e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 114 IDCPIWDqpELMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVY------- 186
Cdd:PRK07867 127 VDSPAWA--DELAAHRDAEPPFRVADPDDLFMLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYvsmplfh 204
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 187 -----QGFSVAFdmsfeeiwisyLVGATLWIaPREITAD---PDalplaLARQGITVLHAVPTLLALFGADVP------- 251
Cdd:PRK07867 205 snavmAGWAVAL-----------AAGASIAL-RRKFSASgflPD-----VRRYGATYANYVGKPLSYVLATPErpddadn 267
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 252 SLRLInLGGEICPPAL---VERWATptrRLFNSYGPTE---------ATVSASLAELRPG----DPVTiGQPLPNyglLV 315
Cdd:PRK07867 268 PLRIV-YGNEGAPGDIarfARRFGC---VVVDGFGSTEggvaitrtpDTPPGALGPLPPGvaivDPDT-GTECPP---AE 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 316 VDAEMKLLPAGATGELC-IIGPGVAEGYLGRPELTAEKFlpnpwaeHAGeprLYRTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:PRK07867 340 DADGRLLNADEAIGELVnTAGPGGFEGYYNDPEADAERM-------RGG---VYWSGDLAYRDADGYAYFAGRLGDWMRV 409
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALSRQLPNGTAAV--VLKPVDGiDQLVAYIVPGGGQPVQIAQLRAQLRET--LPPYMVPSRFELVDTI 470
Cdd:PRK07867 410 DGENLGTAPIERILLRYPDATEVAVyaVPDPVVG-DQVMAALVLAPGAKFDPDAFAEFLAAQpdLGPKQWPSYVRVCAEL 488
|
410
....*....|....*
gi 2772088563 471 PRLTSGKIDRKALRA 485
Cdd:PRK07867 489 PRTATFKVLKRQLSA 503
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
11-484 |
1.40e-19 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 94.14 E-value: 1.40e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 11 ASQPDQTALV--CGALSLSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAE 87
Cdd:PLN02574 50 HNHNGDTALIdsSTGFSISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLG 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 88 RVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGP---------LLRRGGAGPRHP------AYVIYTSGS 152
Cdd:PLN02574 130 EIKKRVVDCSVGLAFTSPENVEKLSPLGVPVIGVPENYDFDSKRiefpkfyelIKEDFDFVPKPVikqddvAAIMYSSGT 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICH----FLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLV--GATLWIAPReitADPDALPL 226
Cdd:PLN02574 210 TGASKGVVLTHRNLIAmvelFVRFEASQYEYPGSDNVYLAALPMFHIYGLSLFVVGLLslGSTIVVMRR---FDASDMVK 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 227 ALARQGITVLHAVPTLLALF--------GADVPSLRLINLGGEICPPALVERW--ATPTRRLFNSYGPTEATVSAS--LA 294
Cdd:PLN02574 287 VIDRFKVTHFPVVPPILMALtkkakgvcGEVLKSLKQVSCGAAPLSGKFIQDFvqTLPHVDFIQGYGMTESTAVGTrgFN 366
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 295 ELRPGDPVTIGQPLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDL 373
Cdd:PLN02574 367 TEKLSKYSSVGLLAPNMQAKVVDWSTgCLLPPGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGW---------LRTGDI 437
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 374 ARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLR 452
Cdd:PLN02574 438 AYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIpVAFVVRRQGSTLSQEAVINYVA 517
|
490 500 510
....*....|....*....|....*....|..
gi 2772088563 453 ETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PLN02574 518 KQVAPYKKVRKVVFVQSIPKSPAGKILRRELK 549
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
10-484 |
1.93e-19 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 93.53 E-value: 1.93e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGAL--SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDAD-TPA 86
Cdd:PRK13390 7 AQIAPDRPAVIVAETgeQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHlTAP 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ER-VMVCleDAQAVGIVTGHGLrEGLDA-IDCPIWDQPELMQKVDG-----PLLrrGGAGPR---HP--AYVIYTSGSTG 154
Cdd:PRK13390 87 EAdYIVG--DSGARVLVASAAL-DGLAAkVGADLPLRLSFGGEIDGfgsfeAAL--AGAGPRlteQPcgAVMLYSSGTTG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 155 KPKGV--------------PINQGAICHFlrsenerlGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPReitAD 220
Cdd:PRK13390 162 FPKGIqpdlpgrdvdapgdPIVAIARAFY--------DISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKR---FD 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 221 PDALPLALARQGITVLHAVPT----LLALFGA-----DVPSLRLINLGGEICP----PALVErWATPTrrLFNSYGPTEA 287
Cdd:PRK13390 231 AQATLGHVERYRITVTQMVPTmfvrLLKLDADvrtryDVSSLRAVIHAAAPCPvdvkHAMID-WLGPI--VYEYYSSTEA 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 288 tvsASLAELRPGD----PVTIGQPLPNyGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEkflpnpwAEHAG 363
Cdd:PRK13390 308 ---HGMTFIDSPDwlahPGSVGRSVLG-DLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAA-------AQHPA 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 364 EPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKP-------VDGIDQLVAYIVP 436
Cdd:PRK13390 377 HPFWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPdpemgeqVKAVIQLVEGIRG 456
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 2772088563 437 GGgqpvQIA-QLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK13390 457 SD----ELArELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
26-485 |
2.38e-19 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 93.53 E-value: 2.38e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTP-------AERVMVCLEDAQA 98
Cdd:PRK09192 50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPLPMGfggresyIAQLRGMLASAQP 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 99 VGIVTGHGLREGLDAIDCPI-------WDQPELMQKVDGPLLRrggAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLR 171
Cdd:PRK09192 130 AAIITPDELLPWVNEATHGNpllhvlsHAWFKALPEADVALPR---PTPDDIAYLQYSSGSTRFPRGVIITHRALMANLR 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 172 S-ENERLGVRADDRVYQGFSVAFDMSfeeiwisyLVGATLWIAPREITADPDAL------PLA----LARQGITVLHAVP 240
Cdd:PRK09192 207 AiSHDGLKVRPGDRCVSWLPFYHDMG--------LVGFLLTPVATQLSVDYLPTrdfarrPLQwldlISRNRGTISYSPP 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 241 -------------TLLALfgaDVPSLRLINLGGEICPP----ALVERWATP--TRRLF-NSYGPTEATVSASLAEL---- 296
Cdd:PRK09192 279 fgyelcarrvnskDLAEL---DLSCWRVAGIGADMIRPdvlhQFAEAFAPAgfDDKAFmPSYGLAEATLAVSFSPLgsgi 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 297 -----------------RPGDP-------VTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPElTAEK 352
Cdd:PRK09192 356 vveevdrdrleyqgkavAPGAEtrrvrtfVNCGKALPGHEIEIRNEAGMPLPERVVGHICVRGPSLMSGYFRDEE-SQDV 434
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 353 FLPNPWAEhageprlyrTGDLARIDEqGQIHCLGRADDQVKVRGFRVELGEIEAAlSRQLPN---GTAAVVLKPVDGIDQ 429
Cdd:PRK09192 435 LAADGWLD---------TGDLGYLLD-GYLYITGRAKDLIIINGRNIWPQDIEWI-AEQEPElrsGDAAAFSIAQENGEK 503
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 430 LVAYIVPGGGQPVQIAQLRAQLRETLppYM---VPSRFELV--DTIPRLTSGKIDRKALRA 485
Cdd:PRK09192 504 IVLLVQCRISDEERRGQLIHALAALV--RSefgVEAAVELVppHSLPRTSSGKLSRAKAKK 562
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
2-484 |
2.39e-19 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 93.55 E-value: 2.39e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA----- 76
Cdd:PRK07059 25 LADLLEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVvvnvn 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 77 --WLPFD-----ADTPAERVMVcLED-----AQAVG-------IVTGHGLREGLDAI-----------DCPIWDQP---- 122
Cdd:PRK07059 105 plYTPRElehqlKDSGAEAIVV-LENfattvQQVLAktavkhvVVASMGDLLGFKGHivnfvvrrvkkMVPAWSLPghvr 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 123 --ELMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKG-VPINQGAICHFLRSE---NERLGVRADDRV---------YQ 187
Cdd:PRK07059 184 fnDALAEGARQTFKPVKLGPDDVAFLQYTGGTTGVSKGaTLLHRNIVANVLQMEawlQPAFEKKPRPDQlnfvcalplYH 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 188 GF--SVAFDMSFEeiwisyLVGATLWIA-PREITAdpdaLPLALARQGITVLHAVPTLL-ALFGA------DVPSLRLIN 257
Cdd:PRK07059 264 IFalTVCGLLGMR------TGGRNILIPnPRDIPG----FIKELKKYQVHIFPAVNTLYnALLNNpdfdklDFSKLIVAN 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 258 LGGEICPPALVERWATPTR-RLFNSYGPTEATVSASLAELRPGD-PVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIG 335
Cdd:PRK07059 334 GGGMAVQRPVAERWLEMTGcPITEGYGLSETSPVATCNPVDATEfSGTIGLPLPSTEVSIRDDDGNDLPLGEPGEICIRG 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 336 PGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNG 415
Cdd:PRK07059 414 PQVMAGYWNRPDETAKVMTADGF---------FRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVL 484
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 416 TAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK07059 485 EVAAVGVPDEHSGEAVKLFVVKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
62-485 |
2.78e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 92.02 E-value: 2.78e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 62 DLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGHGlregldaidcpiwdqpelmqkvDGPLLRRGGAGPR 141
Cdd:PRK08308 44 DIITLVFFLKEKGASVLPIHPDTPKEAAIRMAKRAGCHGLLYGES----------------------DFTKLEAVNYLAE 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFdmsfeeiwiSY------LVGATLWIAPR 215
Cdd:PRK08308 102 EPSLLQYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTH---------SYglicgvLAALTRGSKPV 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 216 EIT-ADPDalpLALARQGIT---VLHAVPTLLALFGADVP---SLRLINLGGEICPPALVERWATPTRRLFNSYGPTEAT 288
Cdd:PRK08308 173 IITnKNPK---FALNILRNTpqhILYAVPLMLHILGRLLPgtfQFHAVMTSGTPLPEAWFYKLRERTTYMMQQYGCSEAG 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 VSASLAELRpgDPVTIGQPLPNYGLLVVDAEmkllpaGATGELCIigpgvaegylgrpeltaekflpnpwaeHAGEPRLY 368
Cdd:PRK08308 250 CVSICPDMK--SHLDLGNPLPHVSVSAGSDE------NAPEEIVV---------------------------KMGDKEIF 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 369 rTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLK---PVDGIDQLVAYIVPGGGQPVQia 445
Cdd:PRK08308 295 -TKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVM-LRLPGVQEAVVYRgkdPVAGERVKAKVISHEEIDPVQ-- 370
|
410 420 430 440
....*....|....*....|....*....|....*....|
gi 2772088563 446 qLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK08308 371 -LREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLLEL 409
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
10-494 |
1.04e-18 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 91.40 E-value: 1.04e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERV 89
Cdd:PLN02860 17 ATLRGNAVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRWSFEEA 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 90 MVCLEDAQAVGIVTGHGLR---EGLDAIDCPIW------DQP---------------ELMQKVDGPLLRRGGAGPRHPAY 145
Cdd:PLN02860 97 KSAMLLVRPVMLVTDETCSswyEELQNDRLPSLmwqvflESPsssvfiflnsfltteMLKQRALGTTELDYAWAPDDAVL 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 VIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDrVY---------QGFSVAFDMsfeeiwisYLVGATLWIAPRe 216
Cdd:PLN02860 177 ICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDD-VYlhtaplchiGGLSSALAM--------LMVGACHVLLPK- 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 217 itADPDALPLALARQGITVLHAVPTLLA---------LFGADVPSLRLINLGGEICPPALVE--RWATPTRRLFNSYGPT 285
Cdd:PLN02860 247 --FDAKAALQAIKQHNVTSMITVPAMMAdlisltrksMTWKVFPSVRKILNGGGSLSSRLLPdaKKLFPNAKLFSAYGMT 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 286 EATVSASLAEL-----------------------RPGDPVTIGQPLPNygllvVDAEMKLLPAGATGELCIIGPGVAEGY 342
Cdd:PLN02860 325 EACSSLTFMTLhdptlespkqtlqtvnqtksssvHQPQGVCVGKPAPH-----VELKIGLDESSRVGRILTRGPHVMLGY 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 343 LGRPELTAEKFLPNPWAEhageprlyrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLK 422
Cdd:PLN02860 400 WGQNSETASVLSNDGWLD---------TGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLS-QHPGVASVVVVG 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 423 PVDG--IDQLVAYI------------VPGGGQPVQIAQ--LRAQLRE-TLPPYMVPSRFEL-VDTIPRLTSGKIDRKALR 484
Cdd:PLN02860 470 VPDSrlTEMVVACVrlrdgwiwsdneKENAKKNLTLSSetLRHHCREkNLSRFKIPKLFVQwRKPFPLTTTGKIRRDEVR 549
|
570
....*....|
gi 2772088563 485 AWPMAALSAT 494
Cdd:PLN02860 550 REVLSHLQSL 559
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
144-484 |
1.71e-18 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 90.66 E-value: 1.71e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 AYVIYTSGSTGKPKGVPINQGAIC-HFLRSENERLGVRADDRVYQGFSVAFDMSFEEI-WISYL-VGATLWIAPReitAD 220
Cdd:cd17642 187 ALIMNSSGSTGLPKGVQLTHKNIVaRFSHARDPIFGNQIIPDTAILTVIPFHHGFGMFtTLGYLiCGFRVVLMYK---FE 263
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 221 PDALPLALARQGITVLHAVPTLLALFGA-------DVPSLRLINLGG-----EIcPPALVERWATPTRRlfNSYGPTEAT 288
Cdd:cd17642 264 EELFLRSLQDYKVQSALLVPTLFAFFAKstlvdkyDLSNLHEIASGGaplskEV-GEAVAKRFKLPGIR--QGYGLTETT 340
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 VSASLAELRPGDPVTIGQPLPNYGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprl 367
Cdd:cd17642 341 SAILITPEGDDKPGAVGKVVPFFYAKVVDLDTgKTLGPNERGELCVKGPMIMKGYVNNPEATKALIDKDGW--------- 411
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 368 YRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVpgggqpvqIAQL 447
Cdd:cd17642 412 LHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVV--------VLEA 483
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 2772088563 448 RAQLRETLPPYMVPSR----------FELVDTIPRLTSGKIDRKALR 484
Cdd:cd17642 484 GKTMTEKEVMDYVASQvstakrlrggVKFVDEVPKGLTGKIDRRKIR 530
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
144-485 |
1.77e-17 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 85.87 E-value: 1.77e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 AYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADdrvyqgfsvafdmsfeeiWISYLVG---ATLWIAPREITA- 219
Cdd:PRK07824 38 ALVVATSGTTGTPKGAMLTAAALTASADATHDRLGGPGQ------------------WLLALPAhhiAGLQVLVRSVIAg 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 220 ------------DPDALPLALARQG----ITVLHAVPTLLALFG-ADVPSLRLIN---LGGEICPPALVERWATPTRRLF 279
Cdd:PRK07824 100 sepveldvsagfDPTALPRAVAELGggrrYTSLVPMQLAKALDDpAATAALAELDavlVGGGPAPAPVLDAAAAAGINVV 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 280 NSYGPTEATvsaslaelrpGDPVTIGQPLPNYGLLVVDaemkllpagatGELCIIGPGVAEGYLGRPEltaekflPNPWA 359
Cdd:PRK07824 180 RTYGMSETS----------GGCVYDGVPLDGVRVRVED-----------GRIALGGPTLAKGYRNPVD-------PDPFA 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 360 EhagePRLYRTGDLARIDEqGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVPGG 438
Cdd:PRK07824 232 E----PGWFRTDDLGALDD-GVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQrVVAAVVGDG 306
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 2772088563 439 GQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK07824 307 GPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
143-479 |
2.14e-17 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 85.05 E-value: 2.14e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAIChflrSENERLGVRAD---DRVYQG----FSVAFDMSfeeIWISYLVGATLWIAPR 215
Cdd:cd17636 2 PVLAIYTAAFSGRPNGALLSHQALL----AQALVLAVLQAideGTVFLNsgplFHIGTLMF---TLATFHAGGTNVFVRR 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 216 eitADPDALPLALARQGIT-------VLHAVPTLLALFGADVPSLRLINLGGEICPPALVerWATPTRRLFNSYGPTEAT 288
Cdd:cd17636 75 ---VDAEEVLELIEAERCThafllppTIDQIVELNADGLYDLSSLRSSPAAPEWNDMATV--DTSPWGRKPGGYGQTEVM 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 289 VSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFLpNPWaehageprlY 368
Cdd:cd17636 150 GLATFAALGGGAIGGAGRPSPLVQVRILDEDGREVPDGEVGEIVARGPTVMAGYWNRPEVNARRTR-GGW---------H 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 369 RTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPN-GTAAVVLKPVDGIDQLV-AYIVPGGGQPVQIAQ 446
Cdd:cd17636 220 HTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCL-RQHPAvADAAVIGVPDPRWAQSVkAIVVLKPGASVTEAE 298
|
330 340 350
....*....|....*....|....*....|...
gi 2772088563 447 LRAQLRETLPPYMVPSRFELVDTIPRLTSGKID 479
Cdd:cd17636 299 LIEHCRARIASYKKPKSVEFADALPRTAGGADD 331
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
25-484 |
2.37e-17 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 87.65 E-value: 2.37e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAA----WLPFDADTPAERVMVCLEDAqavg 100
Cdd:PLN02654 120 SLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVhsvvFAGFSAESLAQRIVDCKPKV---- 195
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 101 IVTGHGLREGLDAID---------------------CPIWDQPELMQKVDGPLLRRGGAG-----PRHPA---------- 144
Cdd:PLN02654 196 VITCNAVKRGPKTINlkdivdaaldesakngvsvgiCLTYENQLAMKREDTKWQEGRDVWwqdvvPNYPTkcevewvdae 275
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 145 ---YVIYTSGSTGKPKGVPINQGAIchflrsenerlgvraddRVYQG--FSVAFDMSFEEI--------WI---SY---- 204
Cdd:PLN02654 276 dplFLLYTSGSTGKPKGVLHTTGGY-----------------MVYTAttFKYAFDYKPTDVywctadcgWItghSYvtyg 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 --LVGATLWI---APReiTADPDALPLALARQGITVLHAVPTLLALFGADVP---------SLRLINLGGE-ICPPALve 269
Cdd:PLN02654 339 pmLNGATVLVfegAPN--YPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDeyvtrhsrkSLRVLGSVGEpINPSAW-- 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 270 RWatptrrLFNSYGPTEATVSASLAELRPG----DPVTIGQP-------LPNYGL--LVVDAEMKLLPAGATGELCIIG- 335
Cdd:PLN02654 415 RW------FFNVVGDSRCPISDTWWQTETGgfmiTPLPGAWPqkpgsatFPFFGVqpVIVDEKGKEIEGECSGYLCVKKs 488
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 336 -PGVAEGYLG---RPELTAEKflpnPWAEHageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQ 411
Cdd:PLN02654 489 wPGAFRTLYGdheRYETTYFK----PFAGY------YFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSH 558
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 412 LPNGTAAVVlkpvdGIDQLV------AYIVPGGGQPVQiAQLRAQL----RETLPPYMVPSRFELVDTIPRLTSGKIDRK 481
Cdd:PLN02654 559 PQCAEAAVV-----GIEHEVkgqgiyAFVTLVEGVPYS-EELRKSLiltvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 632
|
...
gi 2772088563 482 ALR 484
Cdd:PLN02654 633 ILR 635
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
23-485 |
3.61e-17 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 86.72 E-value: 3.61e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 23 ALSLSYRELDERASLMAHHLiKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP-FDADTP--AERVMVCLEDAQAV 99
Cdd:PRK12476 66 AVELTWTQLGVRLRAVGARL-QQVAGPGDRVAILAPQGIDYVAGFFAAIKAGTIAVPlFAPELPghAERLDTALRDAEPT 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 100 GIVTGHGLREGLDAI--DCPIWDQPELMQKVDGPLLRRGGAGPRHP-----AYVIYTSGSTGKPKGVPINQGAICHFLRs 172
Cdd:PRK12476 145 VVLTTTAAAEAVEGFlrNLPRLRRPRVIAIDAIPDSAGESFVPVELdtddvSHLQYTSGSTRPPVGVEITHRAVGTNLV- 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 173 eNERLGVRADDRVYQGFS---VAFDMSFEEIWISYLVG--ATL------------WI--------APREITADPD-ALPL 226
Cdd:PRK12476 224 -QMILSIDLLDRNTHGVSwlpLYHDMGLSMIGFPAVYGghSTLmsptafvrrpqrWIkalsegsrTGRVVTAAPNfAYEW 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 227 ALARqgitvlhAVPTllALFGADVPSLRLINlGGEICPPALVERWAT-------PTRRLFNSYGPTEATVSAS------- 292
Cdd:PRK12476 303 AAQR-------GLPA--EGDDIDLSNVVLII-GSEPVSIDAVTTFNKafapyglPRTAFKPSYGIAEATLFVAtiapdae 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 293 ----------LAELR-----PGDP-----VTIGQPLPNYGLLVVDAEMKL-LPAGATGELCIIGPGVAEGYLGRPELTAE 351
Cdd:PRK12476 373 psvvyldreqLGAGRavrvaADAPnavahVSCGQVARSQWAVIVDPDTGAeLPDGEVGEIWLHGDNIGRGYWGRPEETER 452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 352 KF-----LPNPWAEHAG----EPRLYRTGDLA-RIDeqGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLP---NGTAA 418
Cdd:PRK12476 453 TFgaklqSRLAEGSHADgaadDGTWLRTGDLGvYLD--GELYITGRIADLIVIDGRNHYPQDIEATVAEASPmvrRGYVT 530
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 419 VVLKPVDGIDQLV--AYIVPGGG----QPVQIAqLRAQL--RETLPPYMVpsRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK12476 531 AFTVPAEDNERLVivAERAAGTSradpAPAIDA-IRAAVsrRHGLAVADV--RLVPAGAIPRTTSGKLARRACRA 602
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
22-480 |
4.26e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 85.96 E-value: 4.26e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 22 GALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGI 101
Cdd:cd05914 4 GGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 102 VTGhglregldaidcpiwDQPELmqkvdgpllrrggagprhpAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRA 181
Cdd:cd05914 84 FVS---------------DEDDV-------------------ALINYTSGTTGNSKGVMLTYRNIVSNVDGVKEVVLLGK 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 182 DDRVYQ--GFSVAFDMSFEEIwISYLVGATLWIapreITADPDALPLALARQGITVLHAVPTLLALFG----ADVPSLRL 255
Cdd:cd05914 130 GDKILSilPLHHIYPLTFTLL-LPLLNGAHVVF----LDKIPSAKIIALAFAQVTPTLGVPVPLVIEKifkmDIIPKLTL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 256 INLGGEICPPALVERWATPTRR-LFNSYG-----------PTEATVSASLAELrpGDPVTIG------QPLPNY------ 311
Cdd:cd05914 205 KKFKFKLAKKINNRKIRKLAFKkVHEAFGgnikefviggaKINPDVEEFLRTI--GFPYTIGygmtetAPIISYsppnri 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 312 -----GLLVVDAEMKL---LPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIH 383
Cdd:cd05914 283 rlgsaGKVIDGVEVRIdspDPATGEGEIIVRGPNVMKGYYKNPEATAEAFDKDGW---------FHTGDLGKIDAEGYLY 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 384 CLGRADDQ-VKVRGFRVELGEIEAALSRQLPNGTAAVVLKpvDGIDQLVAYIVP------GGGQPVQIAQL----RAQLR 452
Cdd:cd05914 354 IRGRKKEMiVLSSGKNIYPEEIEAKINNMPFVLESLVVVQ--EKKLVALAYIDPdfldvkALKQRNIIDAIkwevRDKVN 431
|
490 500
....*....|....*....|....*....
gi 2772088563 453 ETLPPYMVPSRFELVDT-IPRLTSGKIDR 480
Cdd:cd05914 432 QKVPNYKKISKVKIVKEeFEKTPKGKIKR 460
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
10-510 |
1.85e-16 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 84.62 E-value: 1.85e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVcgALS--------LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGA----AW 77
Cdd:PRK10524 63 LAKRPEQLALI--AVStetdeertYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAihsvVF 140
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 78 LPFDADTPAERvmvcLEDAQAVGIVTGH-GLREG--------LDAIDCPIWDQPE----------LMQKVDG-----PLL 133
Cdd:PRK10524 141 GGFASHSLAAR----IDDAKPVLIVSADaGSRGGkvvpykplLDEAIALAQHKPRhvllvdrglaPMARVAGrdvdyATL 216
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 134 RRGGAGPR---------HPAYVIYTSGSTGKPKGVPINQGaichflrsenerlgvraddrvyqGFSVAFDMSFEEI---- 200
Cdd:PRK10524 217 RAQHLGARvpvewlesnEPSYILYTSGTTGKPKGVQRDTG-----------------------GYAVALATSMDTIfggk 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 201 ------------WI---SYLVGATLwIA----------PreITADPDALPLALARQGITVLHAVPTLL---------ALF 246
Cdd:PRK10524 274 agetffcasdigWVvghSYIVYAPL-LAgmatimyeglP--TRPDAGIWWRIVEKYKVNRMFSAPTAIrvlkkqdpaLLR 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 GADVPSLRLINLGGEicppALVE---RWATPT--RRLFNSYGPTEA-----TVSASLAEL--RPGDPvtiGQPLPNYGLL 314
Cdd:PRK10524 351 KHDLSSLRALFLAGE----PLDEptaSWISEAlgVPVIDNYWQTETgwpilAIARGVEDRptRLGSP---GVPMYGYNVK 423
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 315 VVDAemkllpagATGELCiiGPG-----VAEGYLGRPELTA-----EKFLPNPWaEHAGEPrLYRTGDLARIDEQGQIHC 384
Cdd:PRK10524 424 LLNE--------VTGEPC--GPNekgvlVIEGPLPPGCMQTvwgddDRFVKTYW-SLFGRQ-VYSTFDWGIRDADGYYFI 491
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 385 LGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVlkpvdGI-DQL-----VAYIVPGGGQPVQIAQLRAQLR------ 452
Cdd:PRK10524 492 LGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVV-----GVkDALkgqvaVAFVVPKDSDSLADREARLALEkeimal 566
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 453 --ETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAwpmaalSATERSESDLPETEAETAL 510
Cdd:PRK10524 567 vdSQLGAVARPARVWFVSALPKTRSGKLLRRAIQA------IAEGRDPGDLTTIEDPAAL 620
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
25-497 |
2.08e-16 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 85.02 E-value: 2.08e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHhLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTG--AAWLPFDAdTPAERVMVClEDAQAVGIV 102
Cdd:PRK06814 658 PLTYRKLLTGAFVLGR-KLKKNTPPGENVGVMLPNANGAAVTFFALQSAGrvPAMINFSA-GIANILSAC-KAAQVKTVL 734
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 103 TG---------HGLREGLDAIDCPIW-----DQPELMQKVDG------PLLRRGGAGPRHPAYVIYTSGSTGKPKGVPIN 162
Cdd:PRK06814 735 TSrafiekarlGPLIEALEFGIRIIYledvrAQIGLADKIKGllagrfPLVYFCNRDPDDPAVILFTSGSEGTPKGVVLS 814
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 163 QGAICHFLRSENERLGVRADDRVYQGFSVaFDmSFEeiwisyLVGATLwiapreitadpdaLPLAlarQGITV------L 236
Cdd:PRK06814 815 HRNLLANRAQVAARIDFSPEDKVFNALPV-FH-SFG------LTGGLV-------------LPLL---SGVKVflypspL 870
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 237 H--AVPTLL------ALFGADV----------P----SLRLINLGGEICPPALVERWATPTR-RLFNSYGPTEATVSASL 293
Cdd:PRK06814 871 HyrIIPELIydtnatILFGTDTflngyaryahPydfrSLRYVFAGAEKVKEETRQTWMEKFGiRILEGYGVTETAPVIAL 950
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 294 ---AELRPGdpvTIGQPLPnyGLlvvdaEMKLLP-AG--ATGELCIIGPGVAEGYLgRPEltaekflpNPWAEHAGEPRL 367
Cdd:PRK06814 951 ntpMHNKAG---TVGRLLP--GI-----EYRLEPvPGidEGGRLFVRGPNVMLGYL-RAE--------NPGVLEPPADGW 1011
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 368 YRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvDGI--DQLVAYIV-PGGGQPVQI 444
Cdd:PRK06814 1012 YDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIP-DARkgERIILLTTaSDATRAAFL 1090
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 445 AQLRAQlreTLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALSATERS 497
Cdd:PRK06814 1091 AHAKAA---GASELMVPAEIITIDEIPLLGTGKIDYVAVTKLAEEAAAKPEAA 1140
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
23-485 |
2.35e-16 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 84.40 E-value: 2.35e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 23 ALSLSYRELDERASLMAHHLiKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLP-FDADTP--AERVMVCLEDAQAV 99
Cdd:PRK07769 53 ARDLTWSQFGARNRAVGARL-QQVTKPGDRVAILAPQNLDYLIAFFGALYAGRIAVPlFDPAEPghVGRLHAVLDDCTPS 131
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 100 GIVTGHGLREGLDAI--DCPIWDQPELMqKVD------GPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLR 171
Cdd:PRK07769 132 AILTTTDSAEGVRKFfrARPAKERPRVI-AVDavpdevGATWVPPEANEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVL 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 172 SENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATL--------------WIapREITADPD------------ALP 225
Cdd:PRK07769 211 QVIDALEGQEGDRGVSWLPFFHDMGLITVLLPALLGHYItfmspaafvrrpgrWI--RELARKPGgtggtfsaapnfAFE 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 226 LALARqgitvlhAVPTllalfgADVPSLRLINL-----GGEICPPALVER-------WATPTRRLFNSYGPTEATVSASL 293
Cdd:PRK07769 289 HAAAR-------GLPK------DGEPPLDLSNVkgllnGSEPVSPASMRKfneafapYGLPPTAIKPSYGMAEATLFVST 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 294 --------------AELRPGDPVTIGQPLPN-------------YGLLVVDAEM-KLLPAGATGELCIIGPGVAEGYLGR 345
Cdd:PRK07769 356 tpmdeeptviyvdrDELNAGRFVEVPADAPNavaqvsagkvgvsEWAVIVDPETaSELPDGQIGEIWLHGNNIGTGYWGK 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 346 PELTAEKF-------LPNPWAEHAGE-PRLYRTGDL-ARIDeqGQIHCLGRADDQVKVRGFRVELGEIE----------- 405
Cdd:PRK07769 436 PEETAATFqnilksrLSESHAEGAPDdALWVRTGDYgVYFD--GELYITGRVKDLVIIDGRNHYPQDLEytaqeatkalr 513
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 406 ----AALS---RQLP-----NGTAAVVLKPVDGIDQLVayIV----PGGG----QPVqIAQLRAQL--RETLPP---YMV 460
Cdd:PRK07769 514 tgyvAAFSvpaNQLPqvvfdDSHAGLKFDPEDTSEQLV--IVaeraPGAHkldpQPI-ADDIRAAIavRHGVTVrdvLLV 590
|
570 580
....*....|....*....|....*
gi 2772088563 461 PSrfelvDTIPRLTSGKIDRKALRA 485
Cdd:PRK07769 591 PA-----GSIPRTSSGKIARRACRA 610
|
|
| EntF2 |
COG3319 |
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase ... |
3-599 |
3.39e-16 |
|
Thioesterase domain of type I polyketide synthase or non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 442548 [Multi-domain] Cd Length: 855 Bit Score: 84.37 E-value: 3.39e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 3 ADLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDA 82
Cdd:COG3319 11 AAAAAAAAAAAAAAAALAAAAAAAAAAALLLLAAALLVALAALALAALALAALLAVALLAAALALAALAALAALALALAA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 83 DTPAERVMVCLEDAQAVGIVTGHGLREGLDAIDCPIWDQPELMQKVDGPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPIN 162
Cdd:COG3319 91 AAAALLLAALALLLALLAALALALLALLLAALLLALAALAAAAAAAALAAAAAAAAALAAAAGLGGGGGGAGVLVLVLAA 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 163 QGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPREITADPDALPLALARQGITVLHAVPTL 242
Cdd:COG3319 171 LLALLLAALLALALALAALLLLALAAALALALLLLLALLLLLLLLLALLLLLLLALLAAAALLALLLALLLLLLAALLLL 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 243 LALFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKL 322
Cdd:COG3319 251 LALALLLLLALLLLLGLLALLLALLLLLALLLLAAAAALAAGGTATTAAVTTTAAAAAPGVAGALGPIGGGPGLLVLLVL 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 323 LPAGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWAEHAGEP-----RLYRTGDLARIDEQGQIHCLGRADDQVKVRGF 397
Cdd:COG3319 331 LVLLLPLLLGVGGGGGGGGGGGGAGGLAGRGLRAAAALRDPAGagargRLRRGGDRGRRLGGGLLLGLGRLRLQRLRRGL 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 398 RVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGK 477
Cdd:COG3319 411 REELEEAEAALAEAAAVAAAVAAAAAAAAAAAALAAAVVAAAALAAAALLLLLLLLLLPPPLPPALLLLLLLLLLLLLAA 490
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 478 IDRKALRAWPMAALSATErsesdlPETEAETALFAALARFFPGLPIQRGGDFFDDLGGHSLLAARLVSALREDGRYAGMT 557
Cdd:COG3319 491 LLLAAAAPAAAAAAAAAP------APAAALELALALLLLLLLGLGLVGDDDDFFGGGGGSLLALLLLLLLLALLLRLLLL 564
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 2772088563 558 VNDVYGQRRVGAIAAAMEAGQARVAAEPMVFATVPRPRRWLC 599
Cdd:COG3319 565 LALLLAPTLAALAAALAAAAAAAALSPLVPLRAGGSGPPLFC 606
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
10-493 |
3.68e-16 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 83.00 E-value: 3.68e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAE-- 87
Cdd:PRK09029 13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPll 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 88 ---------RVMVCLEDAQAVGIVTGHGLREGLDAIDCPiWDqpelmqkvdgpllrrggagPRHPAYVIYTSGSTGKPKg 158
Cdd:PRK09029 93 eellpsltlDFALVLEGENTFSALTSLHLQLVEGAHAVA-WQ-------------------PQRLATMTLTSGSTGLPK- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 159 vpinqgAICHFLRS--ENERlGV------RADDR------VY----QGFsvafdmsfeeIWISYLVGATLWIapreitad 220
Cdd:PRK09029 152 ------AAVHTAQAhlASAE-GVlslmpfTAQDSwllslpLFhvsgQGI----------VWRWLYAGATLVV-------- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 221 PDALPLALARQGITvlHA--VPT----LLAlFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEA--TVSAS 292
Cdd:PRK09029 207 RDKQPLEQALAGCT--HAslVPTqlwrLLD-NRSEPLSLKAVLLGGAAIPVELTEQAEQQGIRCWCGYGLTEMasTVCAK 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 293 LAELRPGdpvtIGQPLPNYGLLVVDaemkllpagatGELCIIGPGVAEGYLGRPELTaekflpnPWAEHAGeprLYRTGD 372
Cdd:PRK09029 284 RADGLAG----VGSPLPGREVKLVD-----------GEIWLRGASLALGYWRQGQLV-------PLVNDEG---WFATRD 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 373 LARIDeQGQIHCLGRADDQVKVRGFRVELGEIEAALSrQLPNGTAAVVLkPVDGID---QLVAYIVPGGGQPVqiAQLRA 449
Cdd:PRK09029 339 RGEWQ-NGELTILGRLDNLFFSGGEGIQPEEIERVIN-QHPLVQQVFVV-PVADAEfgqRPVAVVESDSEAAV--VNLAE 413
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2772088563 450 QLRETLPPYMVPSRFELVDTipRLTSG--KIDRKALRAWPMAALSA 493
Cdd:PRK09029 414 WLQDKLARFQQPVAYYLLPP--ELKNGgiKISRQALKEWVAQQLGN 457
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
201-486 |
4.45e-16 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 82.35 E-value: 4.45e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 201 WISYLVGATLWIAP-----REITADPDALPLalarqgitVLHAVPTLLA-LFGADVPSL---RLINLGGEICPPALVERW 271
Cdd:PRK07445 179 MRSFLTGGKLVILPykrlkSGQELPPNPSDF--------FLSLVPTQLQrLLQLRPQWLaqfRTILLGGAPAWPSLLEQA 250
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 272 ATPTRRLFNSYGPTEatvSAS-LAELRPGDPVT----IGQPLPNygllvvdAEMKLlPAGATGELCIIGPGVAEGYlgrp 346
Cdd:PRK07445 251 RQLQLRLAPTYGMTE---TASqIATLKPDDFLAgnnsSGQVLPH-------AQITI-PANQTGNITIQAQSLALGY---- 315
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 347 eltaekflpnpWAEHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAA-LSRQLPNGTAAVVLKPVD 425
Cdd:PRK07445 316 -----------YPQILDSQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAiLATGLVQDVCVLGLPDPH 384
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 426 GIDQLVAYIVPGGGQPvQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK07445 385 WGEVVTAIYVPKDPSI-SLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQI 444
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
101-485 |
4.66e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 79.69 E-value: 4.66e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 101 IVTGHGLREGLDAIDCP-----IWDQPELMQKVD--GPLLRRGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSE 173
Cdd:PRK13388 103 LVTDAEHRPLLDGLDLPgvrvlDVDTPAYAELVAaaGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRAL 182
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 174 NERLGVRADDRVYQGFSVAFDMSFEEIWISYLV-GATLwIAPREITAD---PDalplaLARQGITVLHAVPTLLALF--- 246
Cdd:PRK13388 183 TERFGLTRDDVCYVSMPLFHSNAVMAGWAPAVAsGAAV-ALPAKFSASgflDD-----VRRYGATYFNYVGKPLAYIlat 256
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 247 -----GADVPsLRlINLGGEICP---PALVERWATptrRLFNSYGPTEATVSASLAELRPgdPVTIGQPLPnyGLLVVDA 318
Cdd:PRK13388 257 perpdDADNP-LR-VAFGNEASPrdiAEFSRRFGC---QVEDGYGSSEGAVIVVREPGTP--PGSIGRGAP--GVAIYNP 327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 319 EMK--------------LLPAGATGELC-IIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIH 383
Cdd:PRK13388 328 ETLtecavarfdahgalLNADEAIGELVnTAGAGFFEGYYNNPEATAERM-RHGM---------YWSGDLAYRDADGWIY 397
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 384 CLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV--VLKPVDGiDQLVAYIVPGGGQ---PVQIAQ-LRAQlrETLPP 457
Cdd:PRK13388 398 FAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVyaVPDERVG-DQVMAALVLRDGAtfdPDAFAAfLAAQ--PDLGT 474
|
410 420
....*....|....*....|....*...
gi 2772088563 458 YMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK13388 475 KAWPRYVRIAADLPSTATNKVLKRELIA 502
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
26-436 |
7.05e-15 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 79.18 E-value: 7.05e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWlprgidllasqlaiAKTGAAWLpfdadTPAERVMvcledAQAVGIVTG- 104
Cdd:cd17639 6 MSYAEVWERVLNFGRGLVELGLKPGDKVAIF--------------AETRAEWL-----ITALGCW-----SQNIPIVTVy 61
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 105 ---------HGLRE-GLDAIDCpiwdqpelmqkvdgpllrrgGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSEN 174
Cdd:cd17639 62 atlgedaliHSLNEtECSAIFT--------------------DGKPDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLG 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 175 ERLG--VRADDRV--YQGFSVAFDMSFEEIWISYlvGATLWIA-PREITADPDALPlalarQG-ITVLHavPTLLAlfga 248
Cdd:cd17639 122 DRVPelLGPDDRYlaYLPLAHIFELAAENVCLYR--GGTIGYGsPRTLTDKSKRGC-----KGdLTEFK--PTLMV---- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 249 DVP-----------------------------SLRLINLGGEICPPALVE------RWAT----------------PTRR 277
Cdd:cd17639 189 GVPaiwdtirkgvlaklnpmgglkrtlfwtayQSKLKALKEGPGTPLLDElvfkkvRAALggrlrymlsggaplsaDTQE 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 278 LFNS--------YGPTEaTVSASlAELRPGDPVT--IGQPLPNygllvvdAEMKLL--PAGAT--------GELCIIGPG 337
Cdd:cd17639 269 FLNIvlcpviqgYGLTE-TCAGG-TVQDPGDLETgrVGPPLPC-------CEIKLVdwEEGGYstdkppprGEILIRGPN 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 338 VAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVR-GFRVELGEIEAAL-SRQLPNG 415
Cdd:cd17639 340 VFKGYYKNPEKTKEAFDGDGW---------FHTGDIGEFHPDGTLKIIDRKKDLVKLQnGEYIALEKLESIYrSNPLVNN 410
|
490 500
....*....|....*....|..
gi 2772088563 416 TAAVvlkpVDGI-DQLVAYIVP 436
Cdd:cd17639 411 ICVY----ADPDkSYPVAIVVP 428
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
26-470 |
3.24e-14 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 76.62 E-value: 3.24e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawlpfdadtpaerVMVCLEDAQavgivTGH 105
Cdd:cd05940 4 LTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGA-------------VAALINYNL-----RGE 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 GLREGLDAidcpiwdqpelmqkvdgpllrrggAGPRH----PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRA 181
Cdd:cd05940 66 SLAHCLNV------------------------SSAKHlvvdAALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALP 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 182 DDRVY--------QGFSVAFDMSFeeiwisyLVGATLWIApREITAD---PDALplalaRQGITVLHAVPTLLALFgADV 250
Cdd:cd05940 122 SDVLYtclplyhsTALIVGWSACL-------ASGATLVIR-KKFSASnfwDDIR-----KYQATIFQYIGELCRYL-LNQ 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 251 P--------SLRLI---NLGGEICpPALVERWATPTRRLFnsYGPTEATVSASLAELRPGDPVTIGQPLP---NYGLLVV 316
Cdd:cd05940 188 PpkpterkhKVRMIfgnGLRPDIW-EEFKERFGVPRIAEF--YAATEGNSGFINFFGKPGAIGRNPSLLRkvaPLALVKY 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 317 DAE-----------MKLLPAGATGEL-CIIGPGVA-EGYLGrPELTAEKFLPNPWAEhaGEpRLYRTGDLARIDEQGQIH 383
Cdd:cd05940 265 DLEsgepirdaegrCIKVPRGEPGLLiSRINPLEPfDGYTD-PAATEKKILRDVFKK--GD-AWFNTGDLMRLDGEGFWY 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 384 CLGRADDQVKVRGFRVELGEIEAALSR------------QLPN-----GTAAVVLKPvdgidqlvayivpggGQPVQIAQ 446
Cdd:cd05940 341 FVDRLGDTFRWKGENVSTTEVAAVLGAfpgveeanvygvQVPGtdgraGMAAIVLQP---------------NEEFDLSA 405
|
490 500
....*....|....*....|....
gi 2772088563 447 LRAQLRETLPPYMVPSRFELVDTI 470
Cdd:cd05940 406 LAAHLEKNLPGYARPLFLRLQPEM 429
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
139-379 |
6.55e-14 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 76.24 E-value: 6.55e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 139 GPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSEnerLGVRADDRVYQ--------------GFSVAFDMSFEEiwisy 204
Cdd:PRK12582 218 TPDTVAKYLFTSGSTGMPKAVINTQRMMCANIAMQ---EQLRPREPDPPppvsldwmpwnhtmGGNANFNGLLWG----- 289
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 lvGATLWIAP---------------REITadPDAL---PLALARQgITVLHAVPTLLALFGADvpsLRLINLGGEICPPA 266
Cdd:PRK12582 290 --GGTLYIDDgkplpgmfeetirnlREIS--PTVYgnvPAGYAML-AEAMEKDDALRRSFFKN---LRLMAYGGATLSDD 361
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 267 LVERW------ATPTRRLFNS-YGPTEA---TVSASLAELRPGdpvTIGQPLPnyGLlvvdaEMKLLPAGATGELCIIGP 336
Cdd:PRK12582 362 LYERMqalavrTTGHRIPFYTgYGATETaptTTGTHWDTERVG---LIGLPLP--GV-----ELKLAPVGDKYEVRVKGP 431
|
250 260 270 280
....*....|....*....|....*....|....*....|....
gi 2772088563 337 GVAEGYLGRPELTAEKFlpnpwaehaGEPRLYRTGDLAR-IDEQ 379
Cdd:PRK12582 432 NVTPGYHKDPELTAAAF---------DEEGFYRLGDAARfVDPD 466
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
10-485 |
8.95e-14 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 75.82 E-value: 8.95e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 10 AASQPDQTAL--VCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAE 87
Cdd:PRK05857 24 ARQQPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIA 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 88 RVMVCLEDAQAVGIVTGHG-------LREGLDAIDCPIWDQPELMQKVDGPLLR-----RGGAGPRHPAYVIYTSGSTGK 155
Cdd:PRK05857 104 AIERFCQITDPAAALVAPGskmassaVPEALHSIPVIAVDIAAVTRESEHSLDAaslagNADQGSEDPLAMIFTSGTTGE 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 156 PKGVPINQG---AICHFLRSEnerlGVRADDRVyQGFSVAFDMSFEEI----WI-SYLVGATLWIAPREITAdpdALPLA 227
Cdd:PRK05857 184 PKAVLLANRtffAVPDILQKE----GLNWVTWV-VGETTYSPLPATHIgglwWIlTCLMHGGLCVTGGENTT---SLLEI 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 228 LARQGITVLHAVPTLLA-------LFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEA--------TVSAS 292
Cdd:PRK05857 256 LTTNAVATTCLVPTLLSklvselkSANATVPSLRLVGYGGSRAIAADVRFIEATGVRTAQVYGLSETgctalclpTDDGS 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 293 LAELRPGdpvTIGQPLPNYGLLVVDAE------MKLLPAGATGELCIIGPGVAEGYLGRPELTAEkFLPNPWAEhagepr 366
Cdd:PRK05857 336 IVKIEAG---AVGRPYPGVDVYLAATDgigptaPGAGPSASFGTLWIKSPANMLGYWNNPERTAE-VLIDGWVN------ 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 367 lyrTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIV------PGGGQ 440
Cdd:PRK05857 406 ---TGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGLAVvasaelDESAA 482
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 2772088563 441 PVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK05857 483 RALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASLAA 527
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
140-382 |
3.98e-13 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 73.79 E-value: 3.98e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 140 PRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSenerlgvraddrVYQGFSVAFDMSFEEIWISYL-------------- 205
Cdd:cd05927 113 PEDLATICYTSGTTGNPKGVMLTHGNIVSNVAG------------VFKILEILNKINPTDVYISYLplahifervvealf 180
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 206 --VGATLWI---APREITADPDAL-PlalarqgiTVLHAVPTLL------------------------------------ 243
Cdd:cd05927 181 lyHGAKIGFysgDIRLLLDDIKALkP--------TVFPGVPRVLnriydkifnkvqakgplkrklfnfalnyklaelrsg 252
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 -----------------ALFGADVpslRLINLGGEICPPALVERW-ATPTRRLFNSYGPTEATVSASLAelRPGDPV--T 303
Cdd:cd05927 253 vvraspfwdklvfnkikQALGGNV---RLMLTGSAPLSPEVLEFLrVALGCPVLEGYGQTECTAGATLT--LPGDTSvgH 327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 304 IGQPLPNYGLLVVD-AEMKLLPAG--ATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQG 380
Cdd:cd05927 328 VGGPLPCAEVKLVDvPEMNYDAKDpnPRGEVCIRGPNVFSGYYKDPEKTAEALDEDGW---------LHTGDIGEWLPNG 398
|
..
gi 2772088563 381 QI 382
Cdd:cd05927 399 TL 400
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
11-478 |
1.21e-12 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 72.30 E-value: 1.21e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 11 ASQPDQTALVCG----ALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPA 86
Cdd:cd05943 80 ADADDPAAIYAAedgeRTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGV 159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 87 ERVMVCLEDAQAVGIVT---------GHGLREGLDAIDCPIWDQPELM--------QKVDGP----------LLRRGGAG 139
Cdd:cd05943 160 PGVLDRFGQIEPKVLFAvdaytyngkRHDVREKVAELVKGLPSLLAVVvvpytvaaGQPDLSkiakaltledFLATGAAG 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 140 PR--------HPAYVIYTSGSTGKPKgvPINQGAICHFLRSENErLGVRAD----DRVYQGFSVAFDMsfeeiW---ISY 204
Cdd:cd05943 240 ELefeplpfdHPLYILYSSGTTGLPK--CIVHGAGGTLLQHLKE-HILHCDlrpgDRLFYYTTCGWMM-----WnwlVSG 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 205 L-VGATLWI---APreITADPDALPLALARQGITVLHAVPT-LLALFGADVP--------SLRLINLGGEICPP---ALV 268
Cdd:cd05943 312 LaVGATIVLydgSP--FYPDTNALWDLADEEGITVFGTSAKyLDALEKAGLKpaethdlsSLRTILSTGSPLKPesfDYV 389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 269 ERWATPTRRLFNSYGPTEatVSASLAELRPGDPVTIGQ-PLPNYGLLVV--DAEMKLLPaGATGELCIIG--PGVAEGYL 343
Cdd:cd05943 390 YDHIKPDVLLASISGGTD--IISCFVGGNPLLPVYRGEiQCRGLGMAVEafDEEGKPVW-GEKGELVCTKpfPSMPVGFW 466
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 344 GRPEltAEKF-------LPNPWAeHageprlyrtGDLARIDEQGQIHCLGRADDQVKVRGFR---------VE-LGEIEA 406
Cdd:cd05943 467 NDPD--GSRYraayfakYPGVWA-H---------GDWIEITPRGGVVILGRSDGTLNPGGVRigtaeiyrvVEkIPEVED 534
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 407 AL--SRQLPNGTAAVVL--KPVDGI---DQLVAYIvpgggqpvqiaqlRAQLRETLPPYMVPSRFELVDTIPRLTSGKI 478
Cdd:cd05943 535 SLvvGQEWKDGDERVILfvKLREGVeldDELRKRI-------------RSTIRSALSPRHVPAKIIAVPDIPRTLSGKK 600
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
26-485 |
1.34e-12 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 72.12 E-value: 1.34e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDA-----------DTPAERVMVC- 92
Cdd:PRK05620 39 TTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKqlmndqivhiiNHAEDEVIVAd 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 LEDAQAVGIVTGH--GLRE----GLDAIDCPIWDQPELMQKVDGPLLRRGGAG--------PRHPAYVIYTSGSTGKPKG 158
Cdd:PRK05620 119 PRLAEQLGEILKEcpCVRAvvfiGPSDADSAAAHMPEGIKVYSYEALLDGRSTvydwpeldETTAAAICYSTGTTGAPKG 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 159 VPINQGAIchFLRSenerLGVRADDrvyqGFSVAFDMSF---EEIW--ISYLVGATLWIAPREIT-ADPDALPLALARQG 232
Cdd:PRK05620 199 VVYSHRSL--YLQS----LSLRTTD----SLAVTHGESFlccVPIYhvLSWGVPLAAFMSGTPLVfPGPDLSAPTLAKII 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 233 ITVL----HAVPT----LLALFGADVP---SLRLINLGGEICPPALVERWAtptRR----LFNSYGPTEATVSASLAelR 297
Cdd:PRK05620 269 ATAMprvaHGVPTlwiqLMVHYLKNPPermSLQEIYVGGSAVPPILIKAWE---ERygvdVVHVWGMTETSPVGTVA--R 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 298 PGDPVTiGQPLPNY----GLLVVDAEMKLLPAGAT--------GELCIIGPGVAEGYLGRP----------------ELT 349
Cdd:PRK05620 344 PPSGVS-GEARWAYrvsqGRFPASLEYRIVNDGQVmestdrneGEIQVRGNWVTASYYHSPteegggaastfrgedvEDA 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 350 AEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVD--GI 427
Cdd:PRK05620 423 NDRFTADGW---------LRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDkwGE 493
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 428 DQLVAYIVPGGGQPVQ--IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK05620 494 RPLAVTVLAPGIEPTRetAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQ 553
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
148-484 |
1.60e-12 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 71.71 E-value: 1.60e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 148 YTSGSTGKPKGVPINQGA-ICHFLRSEN-ERLGVRADDRVY--------QGFSVAFDMSfeeiwisyLVGATLWIAPREI 217
Cdd:PRK06018 184 YTSGTTGDPKGVLYSHRSnVLHALMANNgDALGTSAADTMLpvvplfhaNSWGIAFSAP--------SMGTKLVMPGAKL 255
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 taDPDALPLALARQGITVLHAVPTL-------LALFGADVPSLRLINLGGEICPPALVERWATPTRRLFNSYGPTEATVS 290
Cdd:PRK06018 256 --DGASVYELLDTEKVTFTAGVPTVwlmllqyMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDMGVEVRHAWGMTEMSPL 333
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 291 ASLAELRP------GDP---VTIGQPLPNYG--LLVVDAEMKLLP--AGATGELCIIGPGVAEGYL--GRPELTAEKFlp 355
Cdd:PRK06018 334 GTLAALKPpfsklpGDArldVLQKQGYPPFGveMKITDDAGKELPwdGKTFGRLKVRGPAVAAAYYrvDGEILDDDGF-- 411
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 356 npwaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEaalsrQLPNGTAAVVLKPVDGIdqlvaYIV 435
Cdd:PRK06018 412 ------------FDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLE-----NLAVGHPKVAEAAVIGV-----YHP 469
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 436 PGGGQPVQIAQLRAQ---LRETLPPYM--------VPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PRK06018 470 KWDERPLLIVQLKPGetaTREEILKYMdgkiakwwMPDDVAFVDAIPHTATGKILKTALR 529
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
25-464 |
6.59e-12 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 69.63 E-value: 6.59e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTG--AAWLPFDADtpAERVMVCLEDAQAVGI 101
Cdd:cd05938 5 TYTYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNEPAFLWIWLGLAKLGcpVAFLNTNIR--SKSLLHCFRCCGAKVL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 102 VTGHGLREGLDAIdCP--------IW-----DQPE----LMQKVDG------PLLRRGGAGPRHPAYVIYTSGSTGKPKG 158
Cdd:cd05938 83 VVAPELQEAVEEV-LPalradgvsVWylshtSNTEgvisLLDKVDAasdepvPASLRAHVTIKSPALYIYTSGTTGLPKA 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 159 VPINQG---AICHFLRSenerLGVRADDRVY--------QGFSVAFDMSFEeiwisylVGATLWIAPR----EITADpda 223
Cdd:cd05938 162 ARISHLrvlQCSGFLSL----CGVTADDVIYitlplyhsSGFLLGIGGCIE-------LGATCVLKPKfsasQFWDD--- 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 224 lplalARQ-GITVLHAVptllalfgadvpslrlinlgGEIC------PPALVER----------------WATPTRR--- 277
Cdd:cd05938 228 -----CRKhNVTVIQYI--------------------GELLrylcnqPQSPNDRdhkvrlaignglradvWREFLRRfgp 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 278 --LFNSYGPTEATVSASLAELRPGdpvTIGQP------LPNYGLLVVDAEMKLLPAGATGeLCI-IGPG-----VA---- 339
Cdd:cd05938 283 irIREFYGSTEGNIGFFNYTGKIG---AVGRVsylyklLFPFELIKFDVEKEEPVRDAQG-FCIpVAKGepgllVAkitq 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 340 ----EGYLGRPELTAEKFLPNPWaeHAGEprLY-RTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSR---- 410
Cdd:cd05938 359 qspfLGYAGDKEQTEKKLLRDVF--KKGD--VYfNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLGLldfl 434
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 411 --------QLPN-----GTAAVVLKPvdgidqlvayivpggGQPVQIAQLRAQLRETLPPYMVPsRF 464
Cdd:cd05938 435 qevnvygvTVPGhegriGMAAVKLKP---------------GHEFDGKKLYQHVREYLPAYARP-RF 485
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
8-485 |
1.01e-11 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 69.25 E-value: 1.01e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 8 ATAASQPdqTALVCGALS----LSYRELDERASLMAHHLIKQGVRPGQIVGLwlprgidLLASQLAIAKTG-AAWLPFDA 82
Cdd:PRK07768 10 ANARTSP--RGMVTGEPDapvrHTWGEVHERARRIAGGLAAAGVGPGDAVAV-------LAGAPVEIAPTAqGLWMRGAS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 83 DT----PAERV--MVCLEDAQAVgivtghglregLDAIDCPI------WDQ--PELMQK----------VDGPLLRRGGA 138
Cdd:PRK07768 81 LTmlhqPTPRTdlAVWAEDTLRV-----------IGMIGAKAvvvgepFLAaaPVLEEKgirvltvadlLAADPIDPVET 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 139 GPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRAD-DRVYQGFSVAFDMSFeeiwISYL-----VGATL-W 211
Cdd:PRK07768 150 GEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGM----VGFLtvpmyFGAELvK 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 212 IAPREITADPDALPLALARQGITVLHAVPTLLALFGA-----------DVPSLRLINLGGEICPPALVERWATPTRR--- 277
Cdd:PRK07768 226 VTPMDFLRDPLLWAELISKYRGTMTAAPNFAYALLARrlrrqakpgafDLSSLRFALNGAEPIDPADVEDLLDAGARfgl 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 278 ----LFNSYGPTEATVSAS-----------------LAELRPGDP---------VTIGQPLPNYGLLVVDAEMKLLPAGA 327
Cdd:PRK07768 306 rpeaILPAYGMAEATLAVSfspcgaglvvdevdadlLAALRRAVPatkgntrrlATLGPPLPGLEVRVVDEDGQVLPPRG 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 328 TGELCIIGPGVAEGYlgrpeLTAEKFLP----NPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGE 403
Cdd:PRK07768 386 VGVIELRGESVTPGY-----LTMDGFIPaqdaDGW---------LDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTD 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 404 IEAALSR---QLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLRE--------------TLPPymvpsrfe 465
Cdd:PRK07768 452 IERAAARvegVRPGNAVAVRLDAGHSREGFaVAVESNAFEDPAEVRRIRHQVAHevvaevgvrprnvvVLGP-------- 523
|
570 580
....*....|....*....|
gi 2772088563 466 lvDTIPRLTSGKIDRKALRA 485
Cdd:PRK07768 524 --GSIPKTPSGKLRRANAAE 541
|
|
| PaaK |
COG1541 |
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and ... |
149-480 |
1.17e-11 |
|
Phenylacetate-coenzyme A ligase PaaK, adenylate-forming domain family [Coenzyme transport and metabolism];
Pssm-ID: 441150 [Multi-domain] Cd Length: 423 Bit Score: 68.25 E-value: 1.17e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 149 TSGSTGKPKGVPINQG-------AICHFLRSenerLGVRADDRV-----YQGFSVA--FDMSFEEIwisylvGAT-LWIA 213
Cdd:COG1541 91 SSGTTGKPTVVGYTRKdldrwaeLFARSLRA----AGVRPGDRVqnafgYGLFTGGlgLHYGAERL------GATvIPAG 160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 214 PreitADPDALPLALARQGITVLHAVPT-LLALfgADV----------PSLRLINLGGEICPPALV----ERWATptrRL 278
Cdd:COG1541 161 G----GNTERQLRLMQDFGPTVLVGTPSyLLYL--AEVaeeegidprdLSLKKGIFGGEPWSEEMRkeieERWGI---KA 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 279 FNSYGPTEATVSAS----------------LAELRpgDPVTiGQPLPnygllvvdaemkllpAGATGELCIIGpgvaegy 342
Cdd:COG1541 232 YDIYGLTEVGPGVAyeceaqdglhiwedhfLVEII--DPET-GEPVP---------------EGEEGELVVTT------- 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 343 lgrpeLTAEKFlpnpwaehagePRL-YRTGDLARIDEQ----GQIH-----CLGRADDQVKVRGFRVELGEIEAALsRQL 412
Cdd:COG1541 287 -----LTKEAM-----------PLIrYRTGDLTRLLPEpcpcGRTHprigrILGRADDMLIIRGVNVFPSQIEEVL-LRI 349
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 413 PN--GTAAVVLKPVDGIDQLVAYI--VPGGGQPVQIAQLRAQLRETLppyMVPSRFELV--DTIPRlTSGKIDR 480
Cdd:COG1541 350 PEvgPEYQIVVDREGGLDELTVRVelAPGASLEALAEAIAAALKAVL---GLRAEVELVepGSLPR-SEGKAKR 419
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
274-484 |
1.20e-11 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 68.85 E-value: 1.20e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 274 PTRRLFNSYGPTEAtvsaSLAELRPGDPV---------TIGQPLPNYGLLVVDAEMKL-LPAGATGELCIIGPGVAEGYL 343
Cdd:PLN02330 328 PGVQVQEAYGLTEH----SCITLTHGDPEkghgiakknSVGFILPNLEVKFIDPDTGRsLPKNTPGELCVRSQCVMQGYY 403
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 344 GRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKP 423
Cdd:PLN02330 404 NNKEETDRTIDEDGW---------LHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLP 474
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 424 VDGIDQLVAYIvpgggqpVQIAQLRAQLRETLPPYMVPS--------RFELVDTIPRLTSGKIDRKALR 484
Cdd:PLN02330 475 DEEAGEIPAAC-------VVINPKAKESEEDILNFVAANvahykkvrVVQFVDSIPKSLSGKIMRRLLK 536
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
27-390 |
2.48e-11 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 67.83 E-value: 2.48e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVTGHg 106
Cdd:cd17641 13 TWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAED- 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 107 lREGLDAIdCPIWDQ-----------PELMQKVDGPLLRRGG--------AGPRHP---------------AYVIYTSGS 152
Cdd:cd17641 92 -EEQVDKL-LEIADRipsvryviycdPRGMRKYDDPRLISFEdvvalgraLDRRDPglyerevaagkgedvAVLCTTSGT 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 153 TGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMsfEEIwisYLVGATLW--------------------I 212
Cdd:cd17641 170 TGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIG--EQM---YSVGQALVcgfivnfpeepetmmedlreI 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 213 APREITADP---------------DALPL--ALARQGITVLHAV--------PTLLA-----------LFGADVPSLRLI 256
Cdd:cd17641 245 GPTFVLLPPrvwegiaadvrarmmDATPFkrFMFELGMKLGLRAldrgkrgrPVSLWlrlaswladalLFRPLRDRLGFS 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 257 NL-----GGEICPPALVERW---ATPTRRLfnsYGPTEATVSASLAELRPGDPVTIGQPLPNygllvvdAEMKLlpaGAT 328
Cdd:cd17641 325 RLrsaatGGAALGPDTFRFFhaiGVPLKQL---YGQTELAGAYTVHRDGDVDPDTVGVPFPG-------TEVRI---DEV 391
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 329 GELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGRADD 390
Cdd:cd17641 392 GEILVRSPGVFVGYYKNPEATAEDFDEDGW---------LHTGDAGYFKENGHLVVIDRAKD 444
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
140-484 |
3.74e-11 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 67.13 E-value: 3.74e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 140 PRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLV-GATLWIAP-REI 217
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLAPLIaGMNQYLMPtRLF 184
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 TADPDALPLALARQGITVL----HAVPTLLALFGA------DVPSLRLINLGGEICPPALVERWATPT-----RR--LFN 280
Cdd:cd05908 185 IRRPILWLKKASEHKATIVsspnFGYKYFLKTLKPekandwDLSSIRMILNGAEPIDYELCHEFLDHMskyglKRnaILP 264
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 281 SYGPTEATVSASLAE--------------LRPGDP--------------VTIGQPLPNYGLLVVDAEMKLLPAGATGELC 332
Cdd:cd05908 265 VYGLAEASVGASLPKaqspfktitlgrrhVTHGEPepevdkkdsecltfVEVGKPIDETDIRICDEDNKILPDGYIGHIQ 344
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 333 IIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIdEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQL 412
Cdd:cd05908 345 IRGKNVTPGYYNNPEATAKVFTDDGW---------LKTGDLGFI-RNGRLVITGREKDIIFVNGQNVYPHDIERIAEELE 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 413 PNGTAAVVLKPVDGI----DQLVAYI---------VPGGGQPVQIAQLRA--QLRETLPpymvpsrfelVDTIPRLTSGK 477
Cdd:cd05908 415 GVELGRVVACGVNNSntrnEEIFCFIehrkseddfYPLGKKIKKHLNKRGgwQINEVLP----------IRRIPKTTSGK 484
|
....*..
gi 2772088563 478 IDRKALR 484
Cdd:cd05908 485 VKRYELA 491
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
26-484 |
4.70e-11 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 66.68 E-value: 4.70e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 26 LSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTG--AAWLPFDADTPAerVMVCLEDAQAVGIVT 103
Cdd:cd05939 4 WTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGveTALINSNLRLES--LLHCITVSKAKALIF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 104 GHglregLDAIDCPIWDQPELMQKVDGpllrrggagpRHPAYVIYTSGSTGKPKGvpinqGAICHFlrsenerlgvradd 183
Cdd:cd05939 82 NL-----LDPLLTQSSTEPPSQDDVNF----------RDKLFYIYTSGTTGLPKA-----AVIVHS-------------- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 184 RVYqgfsvafdmsfeeiWISYLVGATLWIAPREITADPdaLPLALARQGI-----TVLHAVPTLL------ALFGADVPS 252
Cdd:cd05939 128 RYY--------------RIAAGAYYAFGMRPEDVVYDC--LPLYHSAGGImgvgqALLHGSTVVIrkkfsaSNFWDDCVK 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 253 LR--LINLGGEIC------PPALVER----------------WATPTRRlFNS------YGPTEATVSASLAELRPGdpv 302
Cdd:cd05939 192 YNctIVQYIGEICryllaqPPSEEEQkhnvrlavgnglrpqiWEQFVRR-FGIpqigefYGATEGNSSLVNIDNHVG--- 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 303 TIGQpLPNYG-------LLVVDAEMKLLPAGATGeLCI-IGPGVA----------------EGYLGRPElTAEKFLPNPW 358
Cdd:cd05939 268 ACGF-NSRILpsvypirLIKVDEDTGELIRDSDG-LCIpCQPGEPgllvgkiiqndplrrfDGYVNEGA-TNKKIARDVF 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 359 AEhaGEpRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAV--VLKP-VDGIDQLVAYIV 435
Cdd:cd05939 345 KK--GD-SAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVygVEVPgVEGRAGMAAIVD 421
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 2772088563 436 PGGGQPVQIaqLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05939 422 PERKVDLDR--FSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQ 468
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
4-486 |
5.84e-11 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 66.89 E-value: 5.84e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 4 DLFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAawlpfdad 83
Cdd:PRK08162 22 SFLERAAEVYPDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGA-------- 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 84 tpaerVMVCLE---DAQAVGIVTGHG--------------LREGLDAIDCPiwdQPELMQKVDGPLlrrgGAGPR----- 141
Cdd:PRK08162 94 -----VLNTLNtrlDAASIAFMLRHGeakvlivdtefaevAREALALLPGP---KPLVIDVDDPEY----PGGRFigald 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 --------HPAYVI-------------YTSGSTGKPKGVpinqgaICH----FLRSenerlgvraddrvyQGFSVAFDMS 196
Cdd:PRK08162 162 yeaflasgDPDFAWtlpadewdaialnYTSGTTGNPKGV------VYHhrgaYLNA--------------LSNILAWGMP 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 197 FEEIwisYLvgATL-------WIAPREITA-----------DPDALPLALARQGITVLHAVPTLLALF---------GAD 249
Cdd:PRK08162 222 KHPV---YL--WTLpmfhcngWCFPWTVAAragtnvclrkvDPKLIFDLIREHGVTHYCGAPIVLSALinapaewraGID 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 250 VPSLRLInlGGEICPPALVERWATPTRRLFNSYGPTE----ATVSASLAE---LRPGDPVTI----GQPLP-NYGLLVVD 317
Cdd:PRK08162 297 HPVHAMV--AGAAPPAAVIAKMEEIGFDLTHVYGLTEtygpATVCAWQPEwdaLPLDERAQLkarqGVRYPlQEGVTVLD 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 318 AE-MKLLPA-GAT-GELCIIGPGVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKV 394
Cdd:PRK08162 375 PDtMQPVPAdGETiGEIMFRGNIVMKGYLKNPKATEEAF-AGGW---------FHTGDLAVLHPDGYIKIKDRSKDIIIS 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 395 RGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQL-VAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELvDTIPRL 473
Cdd:PRK08162 445 GGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVpCAFVELKDGASATEEEIIAHCREHLAGFKVPKAVVF-GELPKT 523
|
570
....*....|...
gi 2772088563 474 TSGKIDRKALRAW 486
Cdd:PRK08162 524 STGKIQKFVLREQ 536
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
403-477 |
1.16e-10 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 58.71 E-value: 1.16e-10
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2772088563 403 EIEAALSRQLPNGTAAVVLKPVDGIDQ-LVAYIVPGGGQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGK 477
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEaPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| AcpA |
COG3433 |
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites ... |
284-582 |
1.94e-10 |
|
Acyl carrier protein/domain [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 442659 [Multi-domain] Cd Length: 295 Bit Score: 63.62 E-value: 1.94e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 284 PTEATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGATGELciigPGVAEGYLGRPELTAEKFLPNPWAEHAG 363
Cdd:COG3433 2 AIATPPPAPPTPDEPPPVIPPAIVQARALLLIVDLQGYFGGFGGEGGL----LGAGLLLRIRLLAAAARAPFIPVPYPAQ 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 364 EPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPVDGIDQLVAYIVPGGGQPVQ 443
Cdd:COG3433 78 PGRQADDLRLLLRRGLGPGGGLERLVQQVVIRAERGEEEELLLVLRAAAVVRVAVLAALRGAGVGLLLIVGAVAALDGLA 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 444 IAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALSATERSESDLPETEAETALFAALARFFPGLP- 522
Cdd:COG3433 158 AAAALAALDKVPPDVVAASAVVALDALLLLALKVVARAAPALAAAEALLAAASPAPALETALTEEELRADVAELLGVDPe 237
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2772088563 523 -IQRGGDFFDdLGGHSLLAARLVSALREDGryAGMTVNDVYGQRRVGAIAAAMEAGQARVA 582
Cdd:COG3433 238 eIDPDDNLFD-LGLDSIRLMQLVERWRKAG--LDVSFADLAEHPTLAAWWALLAAAQAAAA 295
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
1-482 |
1.98e-10 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 64.96 E-value: 1.98e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1 MLADLFEATAASQPDQTALV--------CG-ALSLSYRELDERASLMAHHLIKQGVrPGQIVGLWLPRGIDLLASQLAIA 71
Cdd:PRK05850 2 SVPSLLRERASLQPDDAAFTfidyeqdpAGvAETLTWSQLYRRTLNVAEELRRHGS-TGDRAVILAPQGLEYIVAFLGAL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 72 KTG--AAWLPfdadTPA-----ERVMVCLEDAQ---------AVGIVTGHGLREGLD------AIDCPIWDQPelmqkvD 129
Cdd:PRK05850 81 QAGliAVPLS----VPQggahdERVSAVLRDTSpsvvlttsaVVDDVTEYVAPQPGQsappviEVDLLDLDSP------R 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 130 GPLLRRGGAgpRHPAYVIYTSGSTGKPKGVPIN---------QGAICHFLRSENER-------------------LGVRA 181
Cdd:PRK05850 151 GSDARPRDL--PSTAYLQYTSGSTRTPAGVMVShrnvianfeQLMSDYFGDTGGVPppdttvvswlpfyhdmglvLGVCA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 182 DdrVYQGFSVAF--DMSFEEI---WISYLVgatlwIAPREITADPD-ALPLALARQgitvlhavpTLLALFGADVPSLRL 255
Cdd:PRK05850 229 P--ILGGCPAVLtsPVAFLQRparWMQLLA-----SNPHAFSAAPNfAFELAVRKT---------SDDDMAGLDLGGVLG 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 256 INLGGEICPPALVERWATPTRRlFN--------SYGPTEATV---------------------SASLAELRPGDPvtiGQ 306
Cdd:PRK05850 293 IISGSERVHPATLKRFADRFAP-FNlretairpSYGLAEATVyvatrepgqppesvrfdyeklSAGHAKRCETGG---GT 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 307 PLPNYG------LLVVDAE-MKLLPAGATGELCIIGPGVAEGYLGRPELTAEKF---LPNPWAEHAGEPRLyRTGDLARI 376
Cdd:PRK05850 369 PLVSYGsprsptVRIVDPDtCIECPAGTVGEIWVHGDNVAAGYWQKPEETERTFgatLVDPSPGTPEGPWL-RTGDLGFI 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 377 DEqGQIHCLGRADDQVKVRGFRVELGEIEAALsRQLPNGTAAVVLKPVDGIDQLVAYI---VPGGGQPVQIAQLRAQLRE 453
Cdd:PRK05850 448 SE-GELFIVGRIKDLLIVDGRNHYPDDIEATI-QEITGGRVAAISVPDDGTEKLVAIIelkKRGDSDEEAMDRLRTVKRE 525
|
570 580 590
....*....|....*....|....*....|....*.
gi 2772088563 454 TLPPY-----MVPSRFELV--DTIPRLTSGKIDRKA 482
Cdd:PRK05850 526 VTSAIskshgLSVADLVLVapGSIPITTSGKIRRAA 561
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
14-484 |
9.93e-10 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 62.73 E-value: 9.93e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 14 PDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCL 93
Cdd:PLN03102 28 PNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAIL 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 94 EDAQAVGIVTGHGL----REGLDAIdcPIWD-QPEL----MQKVDGP------------LLRRGGAGP------------ 140
Cdd:PLN03102 108 RHAKPKILFVDRSFeplaREVLHLL--SSEDsNLNLpvifIHEIDFPkrpsseeldyecLIQRGEPTPslvarmfriqde 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 141 RHPAYVIYTSGSTGKPKGVPIN-QGAichFLRSENERLGVRADD-RVYQGFSVAFDMS-FEEIWISYLVGATLwIAPREI 217
Cdd:PLN03102 186 HDPISLNYTSGTTADPKGVVIShRGA---YLSTLSAIIGWEMGTcPVYLWTLPMFHCNgWTFTWGTAARGGTS-VCMRHV 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 218 TAdPDALPlALARQGITVLHAVPTLLALF--------GADVPSLRLINlGGEICPPALVERWATPTRRLFNSYGPTEATV 289
Cdd:PLN03102 262 TA-PEIYK-NIEMHNVTHMCCVPTVFNILlkgnsldlSPRSGPVHVLT-GGSPPPAALVKKVQRLGFQVMHAYGLTEATG 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 290 SASLAELR------PGD---PVTIGQPLPNYGLLVVDAE----MKLLPA-GAT-GELCIIGPGVAEGYLGRPELTAEKFl 354
Cdd:PLN03102 339 PVLFCEWQdewnrlPENqqmELKARQGVSILGLADVDVKnketQESVPRdGKTmGEIVIKGSSIMKGYLKNPKATSEAF- 417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 355 PNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSR----------QLPNGT------AA 418
Cdd:PLN03102 418 KHGW---------LNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKypkvletavvAMPHPTwgetpcAF 488
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 419 VVLKPVD-GIDQLVAYIVPGGGQPVQIAqlraqlRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:PLN03102 489 VVLEKGEtTKEDRVDKLVTRERDLIEYC------RENLPHFMCPRKVVFLQELPKNGNGKILKPKLR 549
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
5-485 |
1.10e-09 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 62.55 E-value: 1.10e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 5 LFEATAASQPDQTALVCGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAW----LPF 80
Cdd:PLN02479 25 FLERAAVVHPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVncvnIRL 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 81 DADTPA--------ERVMVCLE----DAQAVGIVTGHGLREGLDAIDCPIWDQ---PELMQKVdgplLRRGG-------- 137
Cdd:PLN02479 105 NAPTIAfllehsksEVVMVDQEfftlAEEALKILAEKKKSSFKPPLLIVIGDPtcdPKSLQYA----LGKGAieyekfle 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 138 --------AGPRHPAYVI---YTSGSTGKPKGVPIN-QGAicHFLRSENERLGVRADDRVYQGFSVAFDMS-FEEIW-IS 203
Cdd:PLN02479 181 tgdpefawKPPADEWQSIalgYTSGTTASPKGVVLHhRGA--YLMALSNALIWGMNEGAVYLWTLPMFHCNgWCFTWtLA 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 204 YLVGATlwIAPREITADpdALPLALARQGITVLHAVPTLL-ALFGADV-------PSLRLINLGGEICPPALVERWATPT 275
Cdd:PLN02479 259 ALCGTN--ICLRQVTAK--AIYSAIANYGVTHFCAAPVVLnTIVNAPKsetilplPRVVHVMTAGAAPPPSVLFAMSEKG 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 276 RRLFNSYGPTE----ATVSASLAELRPGDPVT------------IGQPlpnyGLLVVDAE-MKLLPA-GAT-GELCIIGP 336
Cdd:PLN02479 335 FRVTHTYGLSEtygpSTVCAWKPEWDSLPPEEqarlnarqgvryIGLE----GLDVVDTKtMKPVPAdGKTmGEIVMRGN 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 337 GVAEGYLGRPELTAEKFlPNPWaehageprlYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGT 416
Cdd:PLN02479 411 MVMKGYLKNPKANEEAF-ANGW---------FHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLE 480
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 417 AAVVLKPVDGI-DQLVAYIVPGGG-----QPVQIAQLRAQLRETLPPYMVPsRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PLN02479 481 ASVVARPDERWgESPCAFVTLKPGvdksdEAALAEDIMKFCRERLPAYWVP-KSVVFGPLPKTATGKIQKHVLRA 554
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
140-486 |
2.25e-09 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 62.03 E-value: 2.25e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 140 PRHPAYVIYTSGSTGKPKGVPINQGAichfLRSENERLGVRAD----DRVYQGFSV--AFDMSFEeIWISYLVGATLWIA 213
Cdd:PRK08043 364 PEDAALILFTSGSEGHPKGVVHSHKS----LLANVEQIKTIADftpnDRFMSALPLfhSFGLTVG-LFTPLLTGAEVFLY 438
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 214 PREITADpdALPLALARQGITVLHAVPTLLALFGA-----DVPSLRLINLGGEICPPALVERWATP-TRRLFNSYGPTEA 287
Cdd:PRK08043 439 PSPLHYR--IVPELVYDRNCTVLFGTSTFLGNYARfanpyDFARLRYVVAGAEKLQESTKQLWQDKfGLRILEGYGVTEC 516
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 288 TVSASLAELRPGDPVTIGQPLPNygllvvdAEMKLLP-AGAT--GELCIIGPGVAEGYLgRPELTAEkfLPNPWAEHAG- 363
Cdd:PRK08043 517 APVVSINVPMAAKPGTVGRILPG-------MDARLLSvPGIEqgGRLQLKGPNIMNGYL-RVEKPGV--LEVPTAENARg 586
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 364 --EPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAAVVLKPvDGI--DQLVAYIVPGGg 439
Cdd:PRK08043 587 emERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKS-DASkgEALVLFTTDSE- 664
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 2772088563 440 qpVQIAQLRAQLRET-LPPYMVPSRFELVDTIPRLTSGKIDRKALRAW 486
Cdd:PRK08043 665 --LTREKLQQYAREHgVPELAVPRDIRYLKQLPLLGSGKPDFVTLKSM 710
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
142-483 |
5.08e-09 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 60.53 E-value: 5.08e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 142 HPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYL-VGATLWIAPREITAD 220
Cdd:PTZ00237 255 HPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVSFHGFLYGSLsLGNTFVMFEGGIIKN 334
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 221 P---DALPLALARQGITVLHAVPTLLALF------------GADVPSLRLINLGGEICPPALVERWATPTR-RLFNSYGP 284
Cdd:PTZ00237 335 KhieDDLWNTIEKHKVTHTLTLPKTIRYLiktdpeatiirsKYDLSNLKEIWCGGEVIEESIPEYIENKLKiKSSRGYGQ 414
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 285 TEATVSASLAELRPGDPV-TIGQPLPNYGLLVVDAEMKLLPAGATGELCI---IGPGVAEGYLGRPELTAEKFlpnpwae 360
Cdd:PTZ00237 415 TEIGITYLYCYGHINIPYnATGVPSIFIKPSILSEDGKELNVNEIGEVAFklpMPPSFATTFYKNDEKFKQLF------- 487
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 361 hAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSRQlPNgtaavVLKPVD-GIDQLVAYIVPGG- 438
Cdd:PTZ00237 488 -SKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKH-PL-----VLECCSiGIYDPDCYNVPIGl 560
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 2772088563 439 --------GQPVQIAQLRAQLRETLP----PYMVPSRFELVDTIPRLTSGKIDRKAL 483
Cdd:PTZ00237 561 lvlkqdqsNQSIDLNKLKNEINNIITqdieSLAVLRKIIIVNQLPKTKTGKIPRQII 617
|
|
| WbbJ |
COG0110 |
Acetyltransferase, isoleucine patch superfamily [General function prediction only]; |
1204-1303 |
7.80e-09 |
|
Acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 439880 [Multi-domain] Cd Length: 140 Bit Score: 55.65 E-value: 7.80e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1204 GVRLGRGVYLDS-TDFTEFDCVSIGDESELNAWS--GPQTHLFED---RIMKIGRIRIGNRVTLGTRSTVLYDTALGDQV 1277
Cdd:COG0110 27 NITIGDNVYIGPgVTIDDPGGITIGDNVLIGPGVtiLTGNHPIDDpatFPLRTGPVTIGDDVWIGAGATILPGVTIGDGA 106
|
90 100
....*....|....*....|....*.
gi 2772088563 1278 QVGPLSLIMKgeSLPARSSWTGSPAR 1303
Cdd:COG0110 107 VVGAGSVVTK--DVPPYAIVAGNPAR 130
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
137-485 |
3.91e-08 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 57.52 E-value: 3.91e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 137 GAGPRHPAYVIYTSGSTGKPKGVPINQGAIChflrsENERLGVRADDRVYQGFSVAFDMSFEEIWIS------YLVGATL 210
Cdd:PRK06334 179 DKDPEDVAVILFTSGTEKLPKGVPLTHANLL-----ANQRACLKFFSPKEDDVMMSFLPPFHAYGFNsctlfpLLSGVPV 253
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 211 WIA-----PREITADPDAlplalarQGITVLHAVPTLLALF-------GADVPSLRLINLGGEICPPALVERWAT--PTR 276
Cdd:PRK06334 254 VFAynplyPKKIVEMIDE-------AKVTFLGSTPVFFDYIlktakkqESCLPSLRFVVIGGDAFKDSLYQEALKtfPHI 326
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 277 RLFNSYGPTEAT-VSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKL-LPAGATGELCIIGPGVAEGYLGRPEltAEKFL 354
Cdd:PRK06334 327 QLRQGYGTTECSpVITINTVNSPKHESCVGMPIRGMDVLIVSEETKVpVSSGETGLVLTRGTSLFSGYLGEDF--GQGFV 404
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 355 pnpwaeHAGEPRLYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALsrqlpngTAAVVLKPVDGIDQLVAYI 434
Cdd:PRK06334 405 ------ELGGETWYVTGDLGYVDRHGELFLKGRLSRFVKIGAEMVSLEALESIL-------MEGFGQNAADHAGPLVVCG 471
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 435 VPGGGQ--------PVQIAQLRAQLRETLPPYMVP-SRFELVDTIPRLTSGKIDRKALRA 485
Cdd:PRK06334 472 LPGEKVrlclfttfPTSISEVNDILKNSKTSSILKiSYHHQVESIPMLGTGKPDYCSLNA 531
|
|
| WbbJ |
COG0110 |
Acetyltransferase, isoleucine patch superfamily [General function prediction only]; |
947-1060 |
5.15e-08 |
|
Acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 439880 [Multi-domain] Cd Length: 140 Bit Score: 53.34 E-value: 5.15e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 947 GVYATVYAPFWYRLLGARIGRNAEIST-AMGLIPDMLTLGDDCFIADGVMLGD-----EEVDGGWMSVDYTVIGPRSFVG 1020
Cdd:COG0110 12 GDGVVIGPGVRIYGGNITIGDNVYIGPgVTIDDPGGITIGDNVLIGPGVTILTgnhpiDDPATFPLRTGPVTIGDDVWIG 91
|
90 100 110 120
....*....|....*....|....*....|....*....|
gi 2772088563 1021 NGAYVPDGTTLPHDVLIGvqSKAPATEQMQPGQTWVGSPA 1060
Cdd:COG0110 92 AGATILPGVTIGDGAVVG--AGSVVTKDVPPYAIVAGNPA 129
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
143-405 |
7.18e-08 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 56.70 E-value: 7.18e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGV-RADDRVYQGFSVAFDMSFEEIWISYLVGATLWIAPreiTADP 221
Cdd:PRK05851 154 PAVLQGTAGSTGTPRTAILSPGAVLSNLRGLNARVGLdAATDVGCSWLPLYHDMGLAFLLTAALAGAPLWLAP---TTAF 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 222 DALPLA----LARQGITVLHAVPTLLALFG--------ADVPSLRLINLGGEICPPALVERWATPTRRL-FN------SY 282
Cdd:PRK05851 231 SASPFRwlswLSDSRATLTAAPNFAYNLIGkyarrvsdVDLGALRVALNGGEPVDCDGFERFATAMAPFgFDagaaapSY 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 283 GPTEATVSASLAELRPG---DPVT------------IGQPLPNYGLLVVDAEMKLLPAGAT-GELCIIGPGVAEGYLGRP 346
Cdd:PRK05851 311 GLAESTCAVTVPVPGIGlrvDEVTtddgsgarrhavLGNPIPGMEVRISPGDGAAGVAGREiGEIEIRGASMMSGYLGQA 390
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 347 ELTaekflPNPWaehageprlYRTGDLARIDEQGQIHClGRADDQVKVRGFRVELGEIE 405
Cdd:PRK05851 391 PID-----PDDW---------FPTGDLGYLVDGGLVVC-GRAKELITVAGRNIFPTEIE 434
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
143-496 |
2.48e-07 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 54.82 E-value: 2.48e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 143 PAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLWI---APreitA 219
Cdd:PLN03051 121 VTNILFSSGTTGEPKAIPWTHLSPLRCASDGWAHMDIQPGDVVCWPTNLGWMMGPWLLYSAFLNGATLALyggAP----L 196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 220 DPDALPLALArQGITVLHAVPTLL---------ALFGADVPSLRLINLGGEICPPALVeRWATPTRRLFNSY----GPTE 286
Cdd:PLN03051 197 GRGFGKFVQD-AGVTVLGLVPSIVkawrhtgafAMEGLDWSKLRVFASTGEASAVDDV-LWLSSVRGYYKPVieycGGTE 274
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 287 -ATVSASLAELRPGDPVTIGQPLPNYGLLVVDAEMKLLPAGAtgelciigPGVAEGYLGRPELTAEKFLPNpwAEH---- 361
Cdd:PLN03051 275 lASGYISSTLLQPQAPGAFSTASLGTRFVLLNDNGVPYPDDQ--------PCVGEVALAPPMLGASDRLLN--ADHdkvy 344
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 362 -AGEPR-------LYRTGDLARIDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSR--QLPNGTAAVVLKPVDG-IDQL 430
Cdd:PLN03051 345 yKGMPMygskgmpLRRHGDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERACDRavAGIAETAAVGVAPPDGgPELL 424
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2772088563 431 VAYIVPG----GGQPVQIAQLRAQ----LRETLPPYMVPSRFELVDTIPRLTSGKIDRKALRAWPMAALSATER 496
Cdd:PLN03051 425 VIFLVLGeekkGFDQARPEALQKKfqeaIQTNLNPLFKVSRVKIVPELPRNASNKLLRRVLRDQLKKELSGRSK 498
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
277-387 |
1.11e-06 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 53.06 E-value: 1.11e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 277 RLFNSYGPTEATVSASLAelRPGD--PVTIGQPLPNygllvvdAEMKLL------------PagaTGELCIIGPGVAEGY 342
Cdd:PTZ00216 454 MVIQGWGLTETVCCGGIQ--RTGDlePNAVGQLLKG-------VEMKLLdteeykhtdtpeP---RGEILLRGPFLFKGY 521
|
90 100 110 120
....*....|....*....|....*....|....*....|....*
gi 2772088563 343 LGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGR 387
Cdd:PTZ00216 522 YKQEELTREVLDEDGW---------FHTGDVGSIAANGTLRIIGR 557
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
27-484 |
1.92e-06 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 52.05 E-value: 1.92e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 27 SYRELDERASLMAHHLIKQ-GVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQAVGIVtgh 105
Cdd:cd05937 7 TYSETYDLVLRYAHWLHDDlGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFVI--- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 106 glregldaidcpiwdqpelmqkVDgpllrrggagPRHPAYVIYTSGSTGKPKGVPINQGAIchfLRSENER---LGVRAD 182
Cdd:cd05937 84 ----------------------VD----------PDDPAILIYTSGTTGLPKAAAISWRRT---LVTSNLLshdLNLKNG 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 183 DRVYQGF----SVAFDMSFEEIWISylvGATLWIAP--------REITADPDALPLALARQGITVLHAVPT-------LL 243
Cdd:cd05937 129 DRTYTCMplyhGTAAFLGACNCLMS---GGTLALSRkfsasqfwKDVRDSGATIIQYVGELCRYLLSTPPSpydrdhkVR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALFGAdvpslrliNLGGEICPpALVERWATPTRRLFnsYGPTEATvsASLAELRPGdPVTIGQpLPNYGLLV-------- 315
Cdd:cd05937 206 VAWGN--------GLRPDIWE-RFRERFNVPEIGEF--YAATEGV--FALTNHNVG-DFGAGA-IGHHGLIRrwkfenqv 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 316 ----VDAE------------MKLLPAGATGELCIIGP----GVAEGYLGRPELTAEKFLPNPWAEhaGEPrLYRTGDLAR 375
Cdd:cd05937 271 vlvkMDPEtddpirdpktgfCVRAPVGEPGEMLGRVPfknrEAFQGYLHNEDATESKLVRDVFRK--GDI-YFRTGDLLR 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 376 IDEQGQIHCLGRADDQVKVRGFRVELGEIEAALSR------------QLPN-----GTAAVVLKPVDGIDqlvayivpgg 438
Cdd:cd05937 348 QDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAhpdiaeanvygvKVPGhdgraGCAAITLEESSAVP---------- 417
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2772088563 439 gQPVQIAQLRAQLRETLPPYMVPSRFELVDTIPRLTSGKIDRKALR 484
Cdd:cd05937 418 -TEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLR 462
|
|
| LbH_MAT_like |
cd04647 |
Maltose O-acyltransferase (MAT)-like: This family is composed of maltose O-acetyltransferase, ... |
1204-1303 |
2.08e-06 |
|
Maltose O-acyltransferase (MAT)-like: This family is composed of maltose O-acetyltransferase, galactoside O-acetyltransferase (GAT), xenobiotic acyltransferase (XAT) and similar proteins. MAT and GAT catalyze the CoA-dependent acetylation of the 6-hydroxyl group of their respective sugar substrates. MAT acetylates maltose and glucose exclusively while GAT specifically acetylates galactopyranosides. XAT catalyzes the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients. Members of this family contain a a left-handed parallel beta-helix (LbH) domain with at least 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). They are trimeric in their active form.
Pssm-ID: 100053 [Multi-domain] Cd Length: 109 Bit Score: 47.84 E-value: 2.08e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1204 GVRLGRGVYLDstDFTEFDC---VSIGDESELnawsGPQTHLF------------EDRIMKIGRIRIGNRVTLGTRSTVL 1268
Cdd:cd04647 1 NISIGDNVYIG--PGCVISAgggITIGDNVLI----GPNVTIYdhnhdiddperpIEQGVTSAPIVIGDDVWIGANVVIL 74
|
90 100 110
....*....|....*....|....*....|....*
gi 2772088563 1269 YDTALGDQVQVGPLSLIMKgeSLPARSSWTGSPAR 1303
Cdd:cd04647 75 PGVTIGDGAVVGAGSVVTK--DVPPNSIVAGNPAK 107
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
2-379 |
2.32e-06 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 51.80 E-value: 2.32e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 2 LADLFEATAASQPDQTALV-----CGALSLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGID----LLASQLAiak 72
Cdd:PRK08180 41 LTDRLVHWAQEAPDRVFLAergadGGWRRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEhallALAAMYA--- 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 73 tGAAWLP------------------FDADTPAervMVCLEDAQA------------VGIVTGHGLREGLDAIDcpiWDqp 122
Cdd:PRK08180 118 -GVPYAPvspayslvsqdfgklrhvLELLTPG---LVFADDGAAfaralaavvpadVEVVAVRGAVPGRAATP---FA-- 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 123 ELMQKVDGPLLRRGGA--GPRHPAYVIYTSGSTGKPKGVPINQGAIC----------HFLRsenERLGVRAD----DRVY 186
Cdd:PRK08180 189 ALLATPPTAAVDAAHAavGPDTIAKFLFTSGSTGLPKAVINTHRMLCanqqmlaqtfPFLA---EEPPVLVDwlpwNHTF 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 187 QGfSVAFDMSFEEiwisylvGATLWIapreitaDpDALPLAlARQGITV--LHAV-PTLL----ALFGADVPSL------ 253
Cdd:PRK08180 266 GG-NHNLGIVLYN-------GGTLYI-------D-DGKPTP-GGFDETLrnLREIsPTVYfnvpKGWEMLVPALerdaal 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 254 --------RLINLGGEICPPAL---VERWATPTR----RLFNSYGPTEATVSASLAELRPGDPVTIGQPLPnyGLlvvda 318
Cdd:PRK08180 329 rrrffsrlKLLFYAGAALSQDVwdrLDRVAEATCgeriRMMTGLGMTETAPSATFTTGPLSRAGNIGLPAP--GC----- 401
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2772088563 319 EMKLLPAGATGELCIIGPGVAEGYLGRPELTAEKFlpnpwaehaGEPRLYRTGDLAR-IDEQ 379
Cdd:PRK08180 402 EVKLVPVGGKLEVRVKGPNVTPGYWRAPELTAEAF---------DEEGYYRSGDAVRfVDPA 454
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
25-159 |
2.58e-06 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 51.69 E-value: 2.58e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERAS-LMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWLPFDADTPAERVMVCLEDAQ------ 97
Cdd:cd17632 67 TITYAELWERVGaVAAAHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEprllav 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 98 -------AVGIVTGHGL----------------REGLDAIDCPIWDQPELMQKVDGPLLRRGGAGPRHP----------A 144
Cdd:cd17632 147 saehldlAVEAVLEGGTpprlvvfdhrpevdahRAALESARERLAAVGIPVTTLTLIAVRGRDLPPAPLfrpepdddplA 226
|
170
....*....|....*
gi 2772088563 145 YVIYTSGSTGKPKGV 159
Cdd:cd17632 227 LLIYTSGSTGTPKGA 241
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
146-409 |
2.86e-06 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 51.56 E-value: 2.86e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 146 VIYTSGSTGKPKGVPINQGAIC-------HFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLVGATLW------- 211
Cdd:PLN02614 228 IMYTSGTTGDPKGVMISNESIVtliagviRLLKSANAALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWrgdvkll 307
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 212 ------IAPREITADPDAL----------------------------PLALARQGITVLHAVPTLLALFGADVP-----S 252
Cdd:PLN02614 308 iedlgeLKPTIFCAVPRVLdrvysglqkklsdggflkkfvfdsafsyKFGNMKKGQSHVEASPLCDKLVFNKVKqglggN 387
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 253 LRLInLGGEICPPALVERW--ATPTRRLFNSYGPTE--ATVSASLAElRPGDPVTIGQPLPNYGLL---VVDAEMKLLPA 325
Cdd:PLN02614 388 VRII-LSGAAPLASHVESFlrVVACCHVLQGYGLTEscAGTFVSLPD-ELDMLGTVGPPVPNVDIRlesVPEMEYDALAS 465
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 326 GATGELCIIGPGVAEGYLGRPELTAEKFLpNPWAeHAGEPRLYR-TGDLARIDEQGQIHCLGRAdDQVKVRGFRVELGEI 404
Cdd:PLN02614 466 TPRGEICIRGKTLFSGYYKREDLTKEVLI-DGWL-HTGDVGEWQpNGSMKIIDRKKNIFKLSQG-EYVAVENIENIYGEV 542
|
....*
gi 2772088563 405 EAALS 409
Cdd:PLN02614 543 QAVDS 547
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
135-375 |
2.06e-05 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 48.97 E-value: 2.06e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 135 RGGAGPRHPAYVIYTSGSTGKPKGVPINQGAICHFLRSENERLGVRADDRVYQGFSVAFDMSFEEIWISYLV---GATLW 211
Cdd:cd05921 159 FAAVGPDTVAKFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEEPPVLVDWLPWNHTFGGNHNFNLVlynGGTLY 238
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 212 IAPREITADPDALPLA-LARQGITVLHAVP--------------TLLALFGADvpsLRLINLGGEICPPALVERW----- 271
Cdd:cd05921 239 IDDGKPMPGGFEETLRnLREISPTVYFNVPagwemlvaalekdeALRRRFFKR---LKLMFYAGAGLSQDVWDRLqalav 315
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 272 ATPTRR--LFNSYGPTE----ATVSASLAElRPGdpvTIGQPLPNygllvvdAEMKLLPAGATGELCIIGPGVAEGYLGR 345
Cdd:cd05921 316 ATVGERipMMAGLGATEtaptATFTHWPTE-RSG---LIGLPAPG-------TELKLVPSGGKYEVRVKGPNVTPGYWRQ 384
|
250 260 270
....*....|....*....|....*....|
gi 2772088563 346 PELTAEKFlpnpwaehaGEPRLYRTGDLAR 375
Cdd:cd05921 385 PELTAQAF---------DEEGFYCLGDAAK 405
|
|
| WbbJ |
COG0110 |
Acetyltransferase, isoleucine patch superfamily [General function prediction only]; |
713-775 |
3.49e-05 |
|
Acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 439880 [Multi-domain] Cd Length: 140 Bit Score: 44.86 E-value: 3.49e-05
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2772088563 713 GARIGADVVIGS-VTVRQPHLLRIGDGASLGSNVNLENV-----RVERGELVVGAIEIGREAYVGSYAV 775
Cdd:COG0110 27 NITIGDNVYIGPgVTIDDPGGITIGDNVLIGPGVTILTGnhpidDPATFPLRTGPVTIGDDVWIGAGAT 95
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
144-409 |
7.56e-05 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 47.03 E-value: 7.56e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 144 AYVIYTSGSTGKPKGVPINQGAICHFLRsenerlGVRAddrvyqgfsVAFDMSFEEIWISYLVGATLWiaprEITADPDA 223
Cdd:PLN02387 253 AVIMYTSGSTGLPKGVMMTHGNIVATVA------GVMT---------VVPKLGKNDVYLAYLPLAHIL----ELAAESVM 313
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 224 L----------PLALA------RQGI---------TVLHAVPTLL----------------------------------- 243
Cdd:PLN02387 314 AavgaaigygsPLTLTdtsnkiKKGTkgdasalkpTLMTAVPAILdrvrdgvrkkvdakgglakklfdiaykrrlaaieg 393
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 244 ALFGA--------DVPSLRLIN--LGGEI----CPPALVerwATPTRRLFN---------SYGPTEATVSASLAElrPGD 300
Cdd:PLN02387 394 SWFGAwglekllwDALVFKKIRavLGGRIrfmlSGGAPL---SGDTQRFINiclgapigqGYGLTETCAGATFSE--WDD 468
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 301 PVT--IGQPLP-NY---------GLLVVDAEMkllpagATGELCIIGPGVAEGYLGRPELTAEKFLpnpwAEHAGEpRLY 368
Cdd:PLN02387 469 TSVgrVGPPLPcCYvklvsweegGYLISDKPM------PRGEIVIGGPSVTLGYFKNQEKTDEVYK----VDERGM-RWF 537
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 2772088563 369 RTGDLARIDEQGQIHCLGRADDQVKVR-GFRVELGEIEAALS 409
Cdd:PLN02387 538 YTGDIGQFHPDGCLEIIDRKKDIVKLQhGEYVSLGKVEAALS 579
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
147-413 |
1.24e-04 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 46.20 E-value: 1.24e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 147 IYTSGSTGKPKGVPINQGAICHFLRSENERLGVR-ADDR-----VYQGFS-VAFDMSfeEIWISYLVGATLWIApreita 219
Cdd:cd05933 156 IYTSGTTGMPKGVMLSHDNITWTAKAASQHMDLRpATVGqesvvSYLPLShIAAQIL--DIWLPIKVGGQVYFA------ 227
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 220 DPDA----LPLALARQGITVLHAVPTL-------LALFGADVPSLR---------------LINLGGEIcPPALVERWA- 272
Cdd:cd05933 228 QPDAlkgtLVKTLREVRPTAFMGVPRVwekiqekMKAVGAKSGTLKrkiaswakgvgletnLKLMGGES-PSPLFYRLAk 306
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 273 -----------------------TPTRR------------LFNSYGPTEATvsaslaelrpgDPVTIGQPLpNYGLLVVD 317
Cdd:cd05933 307 klvfkkvrkalgldrcqkfftgaAPISRetlefflslnipIMELYGMSETS-----------GPHTISNPQ-AYRLLSCG 374
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 318 aemKLLP----------AGATGELCIIGPGVAEGYLGRPELTAEKFLPNPWaehageprlYRTGDLARIDEQGQIHCLGR 387
Cdd:cd05933 375 ---KALPgcktkihnpdADGIGEICFWGRHVFMGYLNMEDKTEEAIDEDGW---------LHSGDLGKLDEDGFLYITGR 442
|
330 340 350
....*....|....*....|....*....|..
gi 2772088563 388 ADDQVKVRGfrvelGE------IEAALSRQLP 413
Cdd:cd05933 443 IKELIITAG-----GEnvppvpIEDAVKKELP 469
|
|
| PRK10502 |
PRK10502 |
putative acyl transferase; Provisional |
684-773 |
1.32e-04 |
|
putative acyl transferase; Provisional
Pssm-ID: 236703 [Multi-domain] Cd Length: 182 Bit Score: 44.17 E-value: 1.32e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 684 WWLADRLhqIAPAYLLSGSSLYNGYLRAMGARIGADVVI-GSVTVRQPHLLRIGDGASLGSNVNLENvrvergelvVGAI 762
Cdd:PRK10502 24 WWAVQAT--LFAWSPQPLYRWRAFLLRLFGAKIGKGVVIrPSVRITYPWKLTIGDYAWIGDDVWLYN---------LGEI 92
|
90
....*....|...
gi 2772088563 763 EIGREAYV--GSY 773
Cdd:PRK10502 93 TIGAHCVIsqKSY 105
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
25-165 |
1.66e-04 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 45.94 E-value: 1.66e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 25 SLSYRELDERASLMAHHLIKQGVRPGQIVGLWLPRGIDLLASQLAIAKTGAAWL---P-FDADTPAER-------VMVC- 92
Cdd:PRK03584 114 ELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSscsPdFGVQGVLDRfgqiepkVLIAv 193
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 93 ----------------------LEDAQAVGIVTGHGLREGLDAIDCPI-WDqpELMQKVDGPLLRRGGAGPRHPAYVIYT 149
Cdd:PRK03584 194 dgyryggkafdrrakvaelraaLPSLEHVVVVPYLGPAAAAAALPGALlWE--DFLAPAEAAELEFEPVPFDHPLWILYS 271
|
170
....*....|....*.
gi 2772088563 150 SGSTGKPKgvPINQGA 165
Cdd:PRK03584 272 SGTTGLPK--CIVHGH 285
|
|
| PaaY |
COG0663 |
Carbonic anhydrase or acetyltransferase, isoleucine patch superfamily [General function ... |
1254-1303 |
2.34e-04 |
|
Carbonic anhydrase or acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 440427 [Multi-domain] Cd Length: 170 Bit Score: 43.09 E-value: 2.34e-04
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1254 RIGNRVTLGTRSTVLYDTALGDQVQVGPLSLIMKGESLPARSSWTGSPAR 1303
Cdd:COG0663 90 TIGDNVLIGMGAIVLDGAVIGDGSIVGAGALVTEGKVVPPGSLVVGSPAK 139
|
|
| AcpP |
COG0236 |
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the ... |
502-579 |
2.37e-04 |
|
Acyl carrier protein [Lipid transport and metabolism]; Acyl carrier protein is part of the Pathway/BioSystem: Fatty acid biosynthesis
Pssm-ID: 440006 [Multi-domain] Cd Length: 80 Bit Score: 40.99 E-value: 2.37e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 502 PETEAETALFAALARFFpGLP---IQRGGDFFDDLGGHSLLAARLVSALREdgRYaGMTVND--VYGQRRVGAIAAAMEA 576
Cdd:COG0236 2 PREELEERLAEIIAEVL-GVDpeeITPDDSFFEDLGLDSLDAVELIAALEE--EF-GIELPDteLFEYPTVADLADYLEE 77
|
...
gi 2772088563 577 GQA 579
Cdd:COG0236 78 KLA 80
|
|
| WbbJ |
COG0110 |
Acetyltransferase, isoleucine patch superfamily [General function prediction only]; |
709-809 |
4.05e-04 |
|
Acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 439880 [Multi-domain] Cd Length: 140 Bit Score: 41.78 E-value: 4.05e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 709 LRAMGARIGADVVIGSVTVRQPHLLRIGDGASLGSNVNLenvrvergeLVVGAIEIGREAYVGSYAVMegdsAIADHGRL 788
Cdd:COG0110 4 LLLFGARIGDGVVIGPGVRIYGGNITIGDNVYIGPGVTI---------DDPGGITIGDNVLIGPGVTI----LTGNHPID 70
|
90 100
....*....|....*....|...
gi 2772088563 789 EGLAALARGG--RIGAReVWDGA 809
Cdd:COG0110 71 DPATFPLRTGpvTIGDD-VWIGA 92
|
|
| PRK05289 |
PRK05289 |
acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase; |
713-775 |
9.38e-04 |
|
acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase;
Pssm-ID: 235390 [Multi-domain] Cd Length: 262 Bit Score: 42.39 E-value: 9.38e-04
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2772088563 713 GARIGADVVIGSVTVRQPHlLRIGDGASLGSNVNLEnvrvergelvvGAIEIGREAYVGSYAV 775
Cdd:PRK05289 14 GAKIGENVEIGPFCVIGPN-VVIGDGTVIGSHVVID-----------GHTTIGKNNRIFPFAS 64
|
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| PaaK |
cd05913 |
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic ... |
232-481 |
1.56e-03 |
|
Phenylacetate-CoA ligase (also known as PaaK); PaaK catalyzes the first step in the aromatic degradation pathway, by converting phenylacetic acid (PA) into phenylacetyl-CoA (PA-CoA). Phenylacetate-CoA ligase has been found in proteobacteria as well as gram positive prokaryotes. The enzyme is specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. PaaKs are members of the adenylate-forming enzyme (AFE) family. However, sequence comparison reveals divergent features of PaaK with respect to the superfamily, including a novel N-terminal sequence.
Pssm-ID: 341239 [Multi-domain] Cd Length: 425 Bit Score: 42.61 E-value: 1.56e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 232 GITVLHAVPTLlALFGADV----------PSLRLINLGGEicppalveRWATPTRRLFNSYGPTEATVSASLAELR-PGD 300
Cdd:cd05913 170 GPTVLCCTPSY-ALYLAEEaeeegidpreLSLKVGIFGAE--------PWTEEMRKRIERRLGIKAYDIYGLTEIIgPGV 240
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90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 301 PVTIGQPL------PNYGLLVVDAEM-KLLPAGATGELCIIgpgvaegylgrpELTAEkflpnpwaehAGEPRLYRTGDL 373
Cdd:cd05913 241 AFECEEKDglhiweDHFIPEIIDPETgEPVPPGEVGELVFT------------TLTKE----------AMPLIRYRTRDI 298
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170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 374 ARIDEQ----GQIH-----CLGRADDQVKVRGFRVELGEIEAALSRQLPNGTAA-VVLKPVDGIDQL-----VAYIVPGG 438
Cdd:cd05913 299 TRLLPGpcpcGRTHrridrITGRSDDMLIIRGVNVFPSQIEDVLLKIPGLGPHYqLILTRQEHLDELtikveVRPEADDD 378
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250 260 270 280
....*....|....*....|....*....|....*....|....*.
gi 2772088563 439 GQPVQIAQ-LRAQLRETLppyMVPSRFELV--DTIPRlTSGKIDRK 481
Cdd:cd05913 379 EKLEALKQrLERHIKSVL---GVTVEVELVepGSLPR-SEGKAKRV 420
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| LbH_WxcM_N_like |
cd03358 |
WcxM-like, Left-handed parallel beta-Helix (LbH) N-terminal domain: This group is composed of ... |
984-1038 |
1.79e-03 |
|
WcxM-like, Left-handed parallel beta-Helix (LbH) N-terminal domain: This group is composed of Xanthomonas campestris WcxM and proteins with similarity to the WcxM N-terminal domain. WcxM is thought to be bifunctional, catalyzing both the isomerization and transacetylation reactions of keto-hexoses. It contains an N-terminal LbH domain responsible for the transacetylation function and a C-terminal isomerase domain. The LbH domain contains imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X), typical of enzymes with acyltransferase activity.
Pssm-ID: 100048 [Multi-domain] Cd Length: 119 Bit Score: 39.41 E-value: 1.79e-03
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*
gi 2772088563 984 LGDDCFIADGVMlgdeeVDGGwmsvdyTVIGPRSFVGNGAYVPDGTTLPHDVLIG 1038
Cdd:cd03358 1 IGDNCIIGTNVF-----IEND------VKIGDNVKIQSNVSIYEGVTIEDDVFIG 44
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| PaaY |
COG0663 |
Carbonic anhydrase or acetyltransferase, isoleucine patch superfamily [General function ... |
983-1060 |
2.57e-03 |
|
Carbonic anhydrase or acetyltransferase, isoleucine patch superfamily [General function prediction only];
Pssm-ID: 440427 [Multi-domain] Cd Length: 170 Bit Score: 40.01 E-value: 2.57e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 983 TLGDDCFIADG--VMlgdeevdggwmsvDYTVIGPRSFVGNGAYVPDGTTLPhdvligvqskapateqmqPGQTWVGSPA 1060
Cdd:COG0663 90 TIGDNVLIGMGaiVL-------------DGAVIGDGSIVGAGALVTEGKVVP------------------PGSLVVGSPA 138
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| LbH_wcaF_like |
cd05825 |
wcaF-like: This group is composed of the protein product of the E. coli wcaF gene and similar ... |
1240-1303 |
2.71e-03 |
|
wcaF-like: This group is composed of the protein product of the E. coli wcaF gene and similar proteins. WcaF is part of the gene cluster responsible for the biosynthesis of the extracellular polysaccharide colanic acid. The wcaF protein is predicted to contain a left-handed parallel beta-helix (LbH) domain encoded by imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing hexapeptide repeats are often enzymes showing acyltransferase activity. Many are trimeric in their active forms.
Pssm-ID: 100063 [Multi-domain] Cd Length: 107 Bit Score: 38.74 E-value: 2.71e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2772088563 1240 THLFEDRIMKI--GRIRIGNRVTLGTRSTVLYDTALGDQVQVGPLSLIMKgeSLPARSSWTGSPAR 1303
Cdd:cd05825 42 SHDYRSPAFPLitAPIVIGDGAWVAAEAFVGPGVTIGEGAVVGARSVVVR--DLPAWTVYAGNPAV 105
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| LbH_gamma_CA_like |
cd04645 |
Gamma carbonic anhydrase-like: This family is composed of gamma carbonic anhydrase (CA), ... |
701-823 |
5.02e-03 |
|
Gamma carbonic anhydrase-like: This family is composed of gamma carbonic anhydrase (CA), Ferripyochelin Binding Protein (FBP), E. coli paaY protein, and similar proteins. CAs are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism, involving the nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Gamma CAs are trimeric enzymes with left-handed parallel beta helix (LbH) structural domain.
Pssm-ID: 100051 [Multi-domain] Cd Length: 153 Bit Score: 38.93 E-value: 5.02e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2772088563 701 GSSL-YNGYLRA-MGA-RIGADVVI--GSV-TVRQPHLLRIGDGASLGSNVNLENVRVERGELV-VGAIeIGREAYVGSY 773
Cdd:cd04645 23 GSSVwFGAVLRGdVNPiRIGERTNIqdGSVlHVDPGYPTIIGDNVTVGHGAVLHGCTIGDNCLIgMGAI-ILDGAVIGKG 101
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|
gi 2772088563 774 AVMEGDSAIAdhgrleglaalaRGGRIGAREVWDGAPAQRQRLWSPGQRQ 823
Cdd:cd04645 102 SIVAAGSLVP------------PGKVIPPGSLVAGSPAKVVRELTDEEIA 139
|
|
| LbH_gamma_CA_like |
cd04645 |
Gamma carbonic anhydrase-like: This family is composed of gamma carbonic anhydrase (CA), ... |
1254-1303 |
5.46e-03 |
|
Gamma carbonic anhydrase-like: This family is composed of gamma carbonic anhydrase (CA), Ferripyochelin Binding Protein (FBP), E. coli paaY protein, and similar proteins. CAs are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism, involving the nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three evolutionary distinct groups - alpha, beta and gamma carbonic anhydrases - which show no significant sequence identity or structural similarity. Gamma CAs are trimeric enzymes with left-handed parallel beta helix (LbH) structural domain.
Pssm-ID: 100051 [Multi-domain] Cd Length: 153 Bit Score: 38.93 E-value: 5.46e-03
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|
gi 2772088563 1254 RIGNRVTLGTRSTVLYDTALGDQVQVGPLSLIMKGESLPARSSWTGSPAR 1303
Cdd:cd04645 79 TIGDNCLIGMGAIILDGAVIGKGSIVAAGSLVPPGKVIPPGSLVAGSPAK 128
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