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Conserved domains on  [gi|955491677|ref|XP_014656749|]
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mitochondrial NAD-dependent isocitrate dehydrogenase subunit 1 precursor [Moesziomyces antarcticus]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
54-388 0e+00

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 552.76  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   54 GGVYTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGETHGSESLFKEAMESLKRNKVGLKGILFTPIETGSHNS 133
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEIPDEAVESIKRNKVALKGPLETPIGKGGHRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  134 WNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAFDFALK 213
Cdd:TIGR00175  81 LNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  214 NGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKssGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALV 293
Cdd:TIGR00175 161 NGRKKVTAVHKANIMKLADGLFLNVCREVAKEYP--DITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLV 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  294 GGPGTVPGCNIGREFALYEPGCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVRTADMGG 373
Cdd:TIGR00175 239 GGPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGG 318

                         330
                  ....*....|....*
gi 955491677  374 KSKTHEFTQAVLSNL 388
Cdd:TIGR00175 319 TATTSDFTEAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 54-388 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 552.76  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   54 GGVYTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGETHGSESLFKEAMESLKRNKVGLKGILFTPIETGSHNS 133
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEIPDEAVESIKRNKVALKGPLETPIGKGGHRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  134 WNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAFDFALK 213
Cdd:TIGR00175  81 LNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  214 NGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKssGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALV 293
Cdd:TIGR00175 161 NGRKKVTAVHKANIMKLADGLFLNVCREVAKEYP--DITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLV 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  294 GGPGTVPGCNIGREFALYEPGCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVRTADMGG 373
Cdd:TIGR00175 239 GGPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGG 318

                         330
                  ....*....|....*
gi 955491677  374 KSKTHEFTQAVLSNL 388
Cdd:TIGR00175 319 TATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 29-388 2.72e-142

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 408.87  E-value: 2.72e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  29 LPQVLRPATTLAAGTPKISKGPTKYGGV-YTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGethGSESLFKEA 107
Cdd:PLN00123   2 LKRLLSNALGSKAQRRSVTYMPRPGDGApRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG---DMKKVPEEV 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 108 MESLKRNKVGLKGILFTPIETGShNSWNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPG 187
Cdd:PLN00123  79 LESIRRNKVCLKGGLATPVGGGV-SSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPG 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 188 VVESLKVSTRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVS 267
Cdd:PLN00123 158 VVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKY--PGIKYNEIIVDNCCMQLVS 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 268 RPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEPGCRHVA---KDIMGTNAANPAAMILSATMMLRHL 344
Cdd:PLN00123 236 KPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNvgnEKLVEQKKANPVALLLSSAMMLRHL 315

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 955491677 345 GLDTQANQIAESVYKVIQDGKVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:PLN00123 316 QFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 57-388 5.79e-128

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 371.65  E-value: 5.79e-128
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEITDSVKEIFEVM----NVPVEWEQFNVSGE---THGsESLFKEAMESLKRNKVGLKGILFTPIETG 129
Cdd:COG0473    2 YKIAVLPGDGIGPEVVAAALKVLEAAaerfGLDFEFEEADIGGAaydKTG-TPLPDETLEALRKADAILLGAVGGPKWDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 130 SHNSW--NVAMRQQLDIYASLVICKSLPGYPTRHK-----DVDFAIIRENTEGEYSGLEHSSYPG----VVESLKVSTRA 198
Cdd:COG0473   81 GVRPEsgLLALRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 199 KAERISRFAFDFALKNgRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVSRPQQFDVMVMP 278
Cdd:COG0473  161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEY--PDVELDHMYVDAAAMQLVRNPEQFDVIVTE 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 279 NLYGNIVSNIGAALVGGPGTVPGCNIGRE-FALYEPgcrhV---AKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIA 354
Cdd:COG0473  238 NLFGDILSDLAAGLTGSLGLAPSANIGDEgKALFEP----VhgsAPDIAGKGIANPIATILSAAMMLRHLGEEEAADAIE 313

                        330       340       350
                 ....*....|....*....|....*....|....
gi 955491677 355 ESVYKVIQDGkVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:COG0473  314 AAVEKVLAEG-VRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
58-384 8.07e-96

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 289.96  E-value: 8.07e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   58 TVTLIPGDGVGVEITDSVKEIFEVMNVPV----EWEQFNVSG---ETHGsESLFKEAMESLKRNKVGLKGILFTPI-ETG 129
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGaaiDETG-EPLPDETLEACKKADAVLLGAVGGPKwDPA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  130 SHNSWN--VAMRQQLDIYASLVICKSLP--GYPTRHK----DVDFAIIRENTEGEYSGLEH---SSYPGVVESLKVSTRA 198
Cdd:pfam00180  80 GVRPENglLALRKELGLFANLRPAKVFPplGDASPLKneveGVDIVIVRELTGGIYFGIEKgikGSGNEVAVDTKLYSRD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  199 KAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKssGIESNDMIVDNTSMQLVSRPQQFDVMVMP 278
Cdd:pfam00180 160 EIERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYP--DVELEHQLVDNAAMQLVKNPSQFDVIVTP 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  279 NLYGNIVSNIGAALVGGPGTVPGCNIG-REFALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRH-LGLDTQANQIAES 356
Cdd:pfam00180 238 NLFGDILSDEASMLTGSLGLLPSASLGaNGFGIFEP-VHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAA 316

                         330       340       350
                  ....*....|....*....|....*....|.
gi 955491677  357 VYKVIQDGKvRTADMGGKS---KTHEFTQAV 384
Cdd:pfam00180 317 VLKVLESGI-RTGDLAGSAtyvSTSEFGEAV 346
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 54-388 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 552.76  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   54 GGVYTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGETHGSESLFKEAMESLKRNKVGLKGILFTPIETGSHNS 133
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEIPDEAVESIKRNKVALKGPLETPIGKGGHRS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  134 WNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAFDFALK 213
Cdd:TIGR00175  81 LNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  214 NGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKssGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALV 293
Cdd:TIGR00175 161 NGRKKVTAVHKANIMKLADGLFLNVCREVAKEYP--DITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLV 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  294 GGPGTVPGCNIGREFALYEPGCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVRTADMGG 373
Cdd:TIGR00175 239 GGPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGG 318

                         330
                  ....*....|....*
gi 955491677  374 KSKTHEFTQAVLSNL 388
Cdd:TIGR00175 319 TATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 29-388 2.72e-142

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 408.87  E-value: 2.72e-142
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  29 LPQVLRPATTLAAGTPKISKGPTKYGGV-YTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGethGSESLFKEA 107
Cdd:PLN00123   2 LKRLLSNALGSKAQRRSVTYMPRPGDGApRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG---DMKKVPEEV 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 108 MESLKRNKVGLKGILFTPIETGShNSWNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPG 187
Cdd:PLN00123  79 LESIRRNKVCLKGGLATPVGGGV-SSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPG 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 188 VVESLKVSTRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVS 267
Cdd:PLN00123 158 VVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKY--PGIKYNEIIVDNCCMQLVS 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 268 RPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEPGCRHVA---KDIMGTNAANPAAMILSATMMLRHL 344
Cdd:PLN00123 236 KPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNvgnEKLVEQKKANPVALLLSSAMMLRHL 315

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 955491677 345 GLDTQANQIAESVYKVIQDGKVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:PLN00123 316 QFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 57-388 5.79e-128

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 371.65  E-value: 5.79e-128
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEITDSVKEIFEVM----NVPVEWEQFNVSGE---THGsESLFKEAMESLKRNKVGLKGILFTPIETG 129
Cdd:COG0473    2 YKIAVLPGDGIGPEVVAAALKVLEAAaerfGLDFEFEEADIGGAaydKTG-TPLPDETLEALRKADAILLGAVGGPKWDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 130 SHNSW--NVAMRQQLDIYASLVICKSLPGYPTRHK-----DVDFAIIRENTEGEYSGLEHSSYPG----VVESLKVSTRA 198
Cdd:COG0473   81 GVRPEsgLLALRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 199 KAERISRFAFDFALKNgRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVSRPQQFDVMVMP 278
Cdd:COG0473  161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEY--PDVELDHMYVDAAAMQLVRNPEQFDVIVTE 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 279 NLYGNIVSNIGAALVGGPGTVPGCNIGRE-FALYEPgcrhV---AKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIA 354
Cdd:COG0473  238 NLFGDILSDLAAGLTGSLGLAPSANIGDEgKALFEP----VhgsAPDIAGKGIANPIATILSAAMMLRHLGEEEAADAIE 313

                        330       340       350
                 ....*....|....*....|....*....|....
gi 955491677 355 ESVYKVIQDGkVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:COG0473  314 AAVEKVLAEG-VRTPDLGGKAGTSEMGDAIIAAL 346
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 24-388 6.24e-127

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 369.98  E-value: 6.24e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  24 LVLPNLPQVLRPATTLAAGTPKISKGPTKYGGVYTVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVSGETHGSESL 103
Cdd:PLN00118   9 LLGNRLAQILGASSSSSGAFSSSARAFSSSSTPITATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTGS 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 104 F--KEAMESLKRNKVGLKGILFTPIETGsHNSWNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLE 181
Cdd:PLN00118  89 FltWESLESVRRNKVGLKGPMATPIGKG-HRSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLE 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 182 HSSYPGVVESLKVSTRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNT 261
Cdd:PLN00118 168 HQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKY--PEIVYEEVIIDNC 245

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 262 SMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGRE-FALYEpGCRHVAKDIMGTNAANPAAMILSATMM 340
Cdd:PLN00118 246 CMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGENgLALAE-AVHGSAPDIAGKNLANPTALLLSAVMM 324

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*...
gi 955491677 341 LRHLGLDTQANQIAESVYKVIQDGKVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:PLN00118 325 LRHLKLNEQAEQIHNAILNTIAEGKYRTADLGGSSTTTDFTKAICDHL 372
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
59-388 5.52e-105

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 317.99  E-value: 5.52e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  59 VTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNV------SGETHGSESlfkEAMESLKRNKVGLKGilftPIET---G 129
Cdd:PRK09222   7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIgekvykKGWTSGISP---SAWESIRRTKVLLKA----PITTpqgG 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 130 SHNSWNVAMRQQLDIYASLVICKSL-PGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAF 208
Cdd:PRK09222  80 GYKSLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAF 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 209 DFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKSsgIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNI 288
Cdd:PRK09222 160 EYARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPD--IEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDI 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 289 GAALVGGPGTVPGCNIGREFALYEP--GCrhvAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGkV 366
Cdd:PRK09222 238 AAEISGSVGLAGSANIGEEYAMFEAvhGS---APDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLEDG-I 313

                        330       340
                 ....*....|....*....|....*...
gi 955491677 367 RTADM--GGKSK----THEFTQAVLSNL 388
Cdd:PRK09222 314 HTADIynEGVSKkkvgTKEFAEAVIENL 341
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 58-388 9.17e-105

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 312.04  E-value: 9.17e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  58 TVTLIPGDGVGVEITDSVKEIFEVMNVPVEWEqFNVSG----ETHGsESLFKEAMESLKRNKVGLKGILFTPIETGsHNS 133
Cdd:PRK08997   4 TITVIPGDGIGPSIIDATLKILDKLGCDFEYE-FADAGltalEKHG-ELLPQRTLDLIEKNKIALKGPLTTPVGEG-FTS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 134 WNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGL--EHSSYPGVVESLKVSTRAKAERISRFAFDFA 211
Cdd:PRK08997  81 INVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEgqTVSEDGETAEATSIITRKGAERIVRFAYELA 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 212 LKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKSsgIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAA 291
Cdd:PRK08997 161 RKEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPD--IEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAG 238

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 292 LVGGPGTVPGCNIGREFALYEpGCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVRTADM 371
Cdd:PRK08997 239 LVGGLGMAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDL 317

                        330
                 ....*....|....*..
gi 955491677 372 GGKSKTHEFTQAVLSNL 388
Cdd:PRK08997 318 GGTHGTTDFTQAVIDRL 334
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
58-384 8.07e-96

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 289.96  E-value: 8.07e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   58 TVTLIPGDGVGVEITDSVKEIFEVMNVPV----EWEQFNVSG---ETHGsESLFKEAMESLKRNKVGLKGILFTPI-ETG 129
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGaaiDETG-EPLPDETLEACKKADAVLLGAVGGPKwDPA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  130 SHNSWN--VAMRQQLDIYASLVICKSLP--GYPTRHK----DVDFAIIRENTEGEYSGLEH---SSYPGVVESLKVSTRA 198
Cdd:pfam00180  80 GVRPENglLALRKELGLFANLRPAKVFPplGDASPLKneveGVDIVIVRELTGGIYFGIEKgikGSGNEVAVDTKLYSRD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  199 KAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKssGIESNDMIVDNTSMQLVSRPQQFDVMVMP 278
Cdd:pfam00180 160 EIERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYP--DVELEHQLVDNAAMQLVKNPSQFDVIVTP 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  279 NLYGNIVSNIGAALVGGPGTVPGCNIG-REFALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRH-LGLDTQANQIAES 356
Cdd:pfam00180 238 NLFGDILSDEASMLTGSLGLLPSASLGaNGFGIFEP-VHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAA 316

                         330       340       350
                  ....*....|....*....|....*....|.
gi 955491677  357 VYKVIQDGKvRTADMGGKS---KTHEFTQAV 384
Cdd:pfam00180 317 VLKVLESGI-RTGDLAGSAtyvSTSEFGEAV 346
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 57-388 8.90e-92

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 278.94  E-value: 8.90e-92
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEITDSVKEIFEVMNVPVEW-------EQFNVSGEThgsesLFKEAMESLKRNKvglkGILFTPI-ET 128
Cdd:PRK14025   2 HKICVIEGDGIGKEVVPAALHVLEATGLPFEFvyaeagdEVFEKTGKA-----LPEETIEAAKEAD----AVLFGAAgET 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 129 GShnSWNVAMRQQLDIYASLVICKSLPGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAF 208
Cdd:PRK14025  73 AA--DVIVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAF 150

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 209 DFALK----NGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKSsgIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNI 284
Cdd:PRK14025 151 EMAKRrkkmGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPD--IKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDI 228

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 285 VSNIGAALVGGPGTVPGCNIGREFALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDG 364
Cdd:PRK14025 229 LSDGAAGLVGGLGLAPSANIGDKYGLFEP-VHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLALG 307

                        330       340
                 ....*....|....*....|....
gi 955491677 365 KVrTADMGGKSKTHEFTQAVLSNL 388
Cdd:PRK14025 308 LT-TPDLGGNLSTMEMAEEVAKRV 330
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 59-385 6.04e-86

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 263.55  E-value: 6.04e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   59 VTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFN---VSGETHGSeSLFKEAMESLKRNKVGLKGILFTPiETGSHNSWN 135
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdEALKKYGS-ALPEDTLEEIRKADAILFGAVTTP-ANPGYKSVI 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  136 VAMRQQLDIYASLVICKSLPGYPTRH-KDVDFAIIRENTEGEYSGLEHSsYPGVVESLKVSTRAKAERISRFAFDFALKN 214
Cdd:TIGR02088  79 VTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFEFG-FSDRAIAIRVITREGSERIARFAFNLAKER 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  215 GRKkVTCVHKANIMKLGDGLFLNTFRRVAEEYkssGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVG 294
Cdd:TIGR02088 158 NRK-VTCVHKANVLKGTDGLFREVCREIAKRY---GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASALAG 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  295 GPGTVPGCNIGREFALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVrTADMGGK 374
Cdd:TIGR02088 234 SLGLAPSANIGDRKALFEP-VHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDLGGT 311

                         330
                  ....*....|.
gi 955491677  375 SKTHEFTQAVL 385
Cdd:TIGR02088 312 AKTKEVGDEIA 322
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 59-388 3.23e-79

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 251.22  E-value: 3.23e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   59 VTLIPGDGVGVEITDSVKEIFEVMNVPVEWEQFNVsGE----THGSESLFKEAMESLKRNKVGLKGILFTPiETGSHNSW 134
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEI-GEkvykKGWPSGISPSSWESIRRTKVLLKAPITTP-QGGGHKSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  135 NVAMRQQLDIYASLVICKSL-PGYPTRHKDVDFAIIRENTEGEYSGLEHSSYPGVVESLKVSTRAKAERISRFAFDFALK 213
Cdd:TIGR02924  81 NVTLRKTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  214 NGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYKSsgIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALV 293
Cdd:TIGR02924 161 HNRKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPD--IESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEIS 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  294 GGPGTVPGCNIGREFALYEpGCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGkVRTADM-- 371
Cdd:TIGR02924 239 GSVGLAGSANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLEDG-VHTADIyn 316

                         330       340
                  ....*....|....*....|.
gi 955491677  372 --GGKSK--THEFTQAVLSNL 388
Cdd:TIGR02924 317 ekTSKQKvgTKEFAEAVTANL 337
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 57-388 2.04e-72

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 229.99  E-value: 2.04e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEITDSVKEIFEVM----NVPVEWEQFNVSG---ETHGS---ESLFKEAMES---------------L 111
Cdd:PRK00772   3 YKIAVLPGDGIGPEVMAEAVKVLDAVaekfGFDFEFEEALVGGaaiDAHGVplpEETLEACRAAdavllgavggpkwdnL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 112 KRNKVGLKGILftpietgshnswnvAMRQQLDIYASL--VIC-KSL----PGYPTRHKDVDFAIIRENTEGEYSGlEHSS 184
Cdd:PRK00772  83 PPDVRPERGLL--------------ALRKELGLFANLrpAKLyPGLadasPLKPEIVAGLDILIVRELTGGIYFG-EPRG 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 185 YPGVVESLK-----VSTRAKAERISRFAFDFALKNgRKKVTCVHKANIMKlGDGLFLNTFRRVAEEYKssGIESNDMIVD 259
Cdd:PRK00772 148 REGLGGEERafdtmVYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYP--DVELSHMYVD 223

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 260 NTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGRE-FALYEP--GCrhvAKDIMGTNAANPAAMILS 336
Cdd:PRK00772 224 NAAMQLVRNPKQFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPihGS---APDIAGKGIANPIATILS 300

                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 955491677 337 ATMMLRH-LGLDTQANQIAESVYKVIQDGkVRTADM---GGKSKTHEFTQAVLSNL 388
Cdd:PRK00772 301 AAMMLRYsLGLEEAADAIEAAVEKVLAQG-YRTADIaegGGKVSTSEMGDAILAAL 355
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 62-388 1.74e-61

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 203.42  E-value: 1.74e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  62 IPGDGVGVEITDSVKEIFE-------VMNVPVEWEQFNVsGETH---GSESLFKEAMESLKRNKVGLKGILFTPIETGsH 131
Cdd:COG0538   24 IEGDGIGPEITRAIWKVIDaavekayGGKRDIEWKEVDA-GEKArdeTGDWLPDETAEAIKEYGVGIKGPLTTPVGGG-W 101

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 132 NSWNVAMRQQLDIYASLVICKSLPGYPTRHK---DVDFAIIRENTEGEYSGLEHSS-YPGVVE----------------- 190
Cdd:COG0538  102 RSLNVTIRQILDLYVCRRPVRYFKGVPSPVKhpeKVDIVIFRENTEDIYAGIEWKAgSPEALKliffledemgvtvirfp 181

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 191 -----SLKVSTRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAE-EYK----------------- 247
Cdd:COG0538  182 edsgiGIKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEeEFGdkfitegpwekykgpkp 261

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 248 SSGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGRE-FALYEPGcrH-VAKDIMGT 325
Cdd:COG0538  262 AGKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGDDgGAEFEAT--HgTAPKYAGK 339

                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 955491677 326 NAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVrTAD----MGGKS--KTHEFTQAVLSNL 388
Cdd:COG0538  340 DSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDlarlMEGATelSTSEFGDAIIENL 407
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 62-388 4.26e-53

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 181.41  E-value: 4.26e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  62 IPGDGVGVEITDSVKEIFEVM-------NVPVEWEQ-------FNVSGEthgSESLFKEAMESLKRNKVGLKGILFTPIe 127
Cdd:PRK07006  25 IEGDGIGPDITPAMLKVVDAAvekaykgERKISWMEiyagekaTKVYGE---DVWLPEETLDLIREYRVAIKGPLTTPV- 100

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 128 TGSHNSWNVAMRQQLDIYASLVICKSLPGYPT---RHKDVDFAIIRENTEGEYSGLEHSSypGVVESLKV---------- 194
Cdd:PRK07006 101 GGGIRSLNVALRQELDLYVCLRPVRYFKGVPSpvkRPEDTDMVIFRENSEDIYAGIEWKA--GSAEAKKVikflqeemgv 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 195 ---------------STRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAE-EY------------ 246
Cdd:PRK07006 179 kkirfpetsgigikpVSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKDWGYQLAEeEFgdelidggpwdk 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 247 ----KSSG-IESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEpgCRH-VAK 320
Cdd:PRK07006 259 iknpETGKeIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIAPGANINDGHAIFE--ATHgTAP 336

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 955491677 321 DIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQdGKVRTAD----MGGKS--KTHEFTQAVLSNL 388
Cdd:PRK07006 337 KYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSMEKTIA-SKTVTYDfarlMEGATevKCSEFGDALIKNM 409
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
62-385 3.86e-52

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 178.87  E-value: 3.86e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  62 IPGDGVGVEITDSVKEifeVMNVPVE----------WEQFNVS--GETHGSESLFKEAMESLKRNKVGLKGILFTPIETG 129
Cdd:PRK06451  29 VEGDGIGPEITHAAMK---VINKAVEkaygsdreikWVEVLAGdkAEKLTGNRFPKESEELIEKYRVLLKGPLETPIGKG 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 130 sHNSWNVAMRQQLDIYASLVICKSLPGY--PTRHKD-VDFAIIRENTEGEYSGLEhssYPGVVES--------------- 191
Cdd:PRK06451 106 -WKSINVAIRLMLDLYANIRPVKYIPGIesPLKNPEkIDLIIFRENTDDLYRGIE---YPYDSEEakkirdflrkelgve 181

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 192 --------LKVSTRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLF--------LNTFRR--VAEE-------- 245
Cdd:PRK06451 182 veddtgigIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFrewayevaLKEFRDyvVTEEevtknyng 261

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 246 -YKSSGIESNDMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEpGCRHVAKDIMG 324
Cdd:PRK06451 262 vPPSGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAG 340

                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 955491677 325 TNAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVrTAD----MGGKS-KTHEFTQAVL 385
Cdd:PRK06451 341 KNVANPTGIIKGGELMLRFMGWDKAADLIDKAIMESIKQKKV-TQDlarfMGVRAlSTTEYTDELI 405
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 59-388 1.11e-51

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 176.05  E-value: 1.11e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677   59 VTLIPGDGVGVEITDS----VKEIFEVMNVPVEWEQFNVSG---ETHGsESLFKEAMESLKRNKVGLKGILFTPI----- 126
Cdd:TIGR00169   2 IAVLPGDGIGPEVMAQalkvLKAVAERFGLKFEFEEHLIGGaaiDATG-QPLPEETLKGCKEADAVLLGAVGGPKwdnlp 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  127 -----ETGShnswnVAMRQQLDIYASLVICKSLPGY----PTRH---KDVDFAIIRENTEGEY----SGLEHSSYPGVVE 190
Cdd:TIGR00169  81 rdqrpEQGL-----LKLRKSLDLFANLRPAKVFPGLedlsPLKEeiaKGVDFVVVRELTGGIYfgepKGREGEGEAWDTE 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  191 slkVSTRAKAERISRFAFDFALKNgRKKVTCVHKANIMKLGDgLFLNTFRRVAEEYKSsgIESNDMIVDNTSMQLVSRPQ 270
Cdd:TIGR00169 156 ---VYTVPEIERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPD--VELEHQYIDNAAMQLVKSPT 228

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  271 QFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEPGCRHVAKDIMGTNAANPAAMILSATMMLRH-LGLDTQ 349
Cdd:TIGR00169 229 QFDVVVTSNLFGDILSDEASVITGSLGMLPSASLGSDGFGLFEPVHGSAPDIAGKGIANPIAQILSAAMLLRYsFNLEEA 308

                         330       340       350
                  ....*....|....*....|....*....|....*....
gi 955491677  350 ANQIAESVYKVIQDGKvRTADMGGKSKTHEFTQAVLSNL 388
Cdd:TIGR00169 309 ADAIEAAVKKVLAEGY-RTPDLGSSATTAVGTAEMGEEL 346
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 58-388 1.75e-51

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 175.68  E-value: 1.75e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  58 TVTLIPGDGVGVEI----TDSVKEIFEVM-NVPVEWEQFNVSGE---THGsESLFKEAMESLKRNKVGLKGILFTPIETG 129
Cdd:PRK08194   5 KIAVIPGDGVGKEVvpaaVRVLKAVAEVHgGLKFEFTEFPWSCEyylEHG-EMMPEDGLEQLKQFDAIFLGAVGNPKLVP 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 130 SHNS-WN--VAMRQQLDIYASLVICKSLPGY--PTRH-KDVDFAIIRENTEGEYS---GLEHSSYPGVVESLKVSTRAKA 200
Cdd:PRK08194  84 DHISlWGllIKIRREFEQVINIRPAKQLRGIksPLANpKDFDLLVVRENSEGEYSevgGRIHRGEDEIAIQNAVFTRKGT 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 201 ERISRFAFDFAlKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVSRPQQFDVMVMPNL 280
Cdd:PRK08194 164 ERAMRYAFELA-AKRRKHVTSATKSNGIVHSMPFWDEVFQEVGKDY--PEIETDSQHIDALAAFFVTRPEEFDVIVASNL 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 281 YGNIVSNIGAALVGGPGTVPGCNI---GREFALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRHLGLDTQANQIAESV 357
Cdd:PRK08194 241 FGDILTDIGAAIMGSIGIAPAANInvnGKYPSMFEP-VHGSAPDIAGKGIANPIGQIWTAKLMLDHFGEEELGSHLLDVI 319

                        330       340       350
                 ....*....|....*....|....*....|.
gi 955491677 358 YKVIQDGkVRTADMGGKSKTHEFTQAVLSNL 388
Cdd:PRK08194 320 EDVTEDG-IKTPDIGGRATTDEVTDEIISRL 349
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 57-372 1.26e-50

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 173.19  E-value: 1.26e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEItdsVKEIFEVMN------VPVEWEQFNVSGETH--GSESLFKEAMESLKRNKVGLKGILFTP--- 125
Cdd:PRK03437   5 MKLAVIPGDGIGPEV---VAEALKVLDavaaggPGVETTEYDLGARRYlrTGETLPDSVLAELRQHDAILLGAIGDPsvp 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 126 ---IETGshnsWNVAMRQQLDIYASLVICKSLPGYPTRHK---DVDFAIIRENTEGEYSGLEHSSYPG----VVESLKVS 195
Cdd:PRK03437  82 sgvLERG----LLLKLRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGNGGALRVGtpheVATEVSVN 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 196 TRAKAERISRFAFDFALKNGRKKVTCVHKANIMKLGDGLFLNTFRRVAEEYksSGIESNDMIVDNTSMQLVSRPQQFDVM 275
Cdd:PRK03437 158 TAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEY--PDVTVDYQHVDAATIFMVTDPSRFDVI 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 276 VMPNLYGNIVSNIGAALVGGPGTVPGCNI---GREFALYEP--GCrhvAKDIMGTNAANPAAMILSATMMLRHLGLDTQA 350
Cdd:PRK03437 236 VTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFEPvhGS---APDIAGQGIADPTAAILSVALLLDHLGEEDAA 312

                        330       340
                 ....*....|....*....|....*
gi 955491677 351 NQIAESVYKVIQ---DGKVRTADMG 372
Cdd:PRK03437 313 ARIEAAVEADLAergKMGRSTAEVG 337
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
38-387 2.08e-33

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 129.83  E-value: 2.08e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  38 TLAAGTPKISKGPTkyggvytVTLIPGDGVGVEITDSVKEIFEVM-------NVPVEWeqFNV-----SGETHGS-ESLF 104
Cdd:PRK07362  17 TFKNGKPVVPDNPI-------IPFIRGDGTGVDIWPATQKVLDAAvakayggERKINW--FKVyagdeACDLYGTyQYLP 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 105 KEAMESLKRNKVGLKGILFTPIeTGSHNSWNVAMRQQLDIYASLVICKSLPGYPTRHK---DVDFAIIRENTEGEYSGLE 181
Cdd:PRK07362  88 EDTLEAIREYGVAIKGPLTTPI-GGGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGIE 166

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 182 HSSYPGVVESL----------------------------KVSTRAKAERISRFAFDFALK-NGRKK-VTCVHKANIMKLG 231
Cdd:PRK07362 167 WEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEHALRlPGDKRhVTLVHKGNIMKYT 246

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 232 DGLF--------LNTFRRVAEEYKSSGIESN------------------------------------------------- 254
Cdd:PRK07362 247 EGAFrdwgyelaTTEFRDECVTERESWILSNkeknpnisiednarmiepgydsltpekkaaicaevkevldsiwsshgng 326

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 255 ---------DMIVDNTSMQLVSRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREFALYEpGCRHVAKDIMGT 325
Cdd:PRK07362 327 kwkekvlvdDRIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAGL 405

                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 955491677 326 NAANPAAMILSATMMLRHLGLDTQANQIAESVYKVIQDGKVrTADMG-------GKSKTHEFTQAVLSN 387
Cdd:PRK07362 406 DRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIANKQV-TYDLArlmeppvDPLSCSEFAEAIISH 473
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 57-371 1.25e-25

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 107.08  E-value: 1.25e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677  57 YTVTLIPGDGVGVEITDSVKEI-----------FEVMNVPVEWEQFNVSGEthgseSLFKEAMESLKRNKVGLKGIL--F 123
Cdd:PLN02329  47 YNIALLPGDGIGPEVISVAKNVlqkagslegleFDFQEMPVGGAALDLVGV-----PLPEETFTAAKQSDAILLGAIggY 121

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 124 TPIETGSHNSWNVAM---RQQLDIYASLVICKSLPGY---PTRHKDV----DFAIIRENTEGEYSGL-------EHSSYP 186
Cdd:PLN02329 122 KWDKNEKHLRPEMALfylRRDLKVFANLRPATVLPQLvdaSTLKKEVaegvDMMIVRELTGGIYFGEprgitinENGEEV 201

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 187 GVveSLKVSTRAKAERISRFAFDFALKNgRKKVTCVHKANIMklgDGLFLNTFRRVAEEYKSSGIESNDMIVDNTSMQLV 266
Cdd:PLN02329 202 GV--STEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVL---DASILWRKRVTALASEYPDVELSHMYVDNAAMQLI 275

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 955491677 267 SRPQQFDVMVMPNLYGNIVSNIGAALVGGPGTVPGCNIGREF-ALYEPgCRHVAKDIMGTNAANPAAMILSATMMLRH-L 344
Cdd:PLN02329 276 RDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGpGLFEP-IHGSAPDIAGQDKANPLATILSAAMLLKYgL 354

                        330       340
                 ....*....|....*....|....*..
gi 955491677 345 GLDTQANQIAESVYKVIQDGkVRTADM 371
Cdd:PLN02329 355 GEEKAAKRIEDAVVDALNKG-FRTGDI 380
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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