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Conserved domains on  [gi|2217347096|ref|XP_047305230|]
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DDB1- and CUL4-associated factor 1 isoform X2 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1304 7.23e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 61.97  E-value: 7.23e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200     51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200    112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2217347096 1247 RSAQAIHKFDKFNMNISGV-FHPNGLE------------WDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1304
Cdd:cd00200    165 RTGKCVATLTGHTGEVNSVaFSPDGEKllssssdgtiklWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1304 7.23e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 61.97  E-value: 7.23e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200     51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200    112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2217347096 1247 RSAQAIHKFDKFNMNISGV-FHPNGLE------------WDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1304
Cdd:cd00200    165 RTGKCVATLTGHTGEVNSVaFSPDGEKllssssdgtiklWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1300 4.80e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 4.80e-09
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLltsATWSQPLSA-LW---GMKSVFDM 1172
Cdd:COG2319    166 TSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLL---ASGSADGTVrLWdlaTGKLLRTL 242

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 KHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVR 1247
Cdd:COG2319    243 TGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTL----TGHSGGVNSVAFSPDGKLLAsgsDDGTvrLWDLA 318

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2217347096 1248 SAQAIHKFDKFNMNISGV-FHPNGLE------------WDLRTFHLLHTVPAldqcrvvfnHTGTV 1300
Cdd:COG2319    319 TGKLLRTLTGHTGAVRSVaFSPDGKTlasgsddgtvrlWDLATGELLRTLTG---------HTGAV 375
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1304 7.23e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 61.97  E-value: 7.23e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200     51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200    112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2217347096 1247 RSAQAIHKFDKFNMNISGV-FHPNGLE------------WDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1304
Cdd:cd00200    165 RTGKCVATLTGHTGEVNSVaFSPDGEKllssssdgtiklWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1300 4.80e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 4.80e-09
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLltsATWSQPLSA-LW---GMKSVFDM 1172
Cdd:COG2319    166 TSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLL---ASGSADGTVrLWdlaTGKLLRTL 242

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 KHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVR 1247
Cdd:COG2319    243 TGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTL----TGHSGGVNSVAFSPDGKLLAsgsDDGTvrLWDLA 318

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2217347096 1248 SAQAIHKFDKFNMNISGV-FHPNGLE------------WDLRTFHLLHTVPAldqcrvvfnHTGTV 1300
Cdd:COG2319    319 TGKLLRTLTGHTGAVRSVaFSPDGKTlasgsddgtvrlWDLATGELLRTLTG---------HTGAV 375
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1286 5.17e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 57.23  E-value: 5.17e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSAT------WS----QPLSALWG- 1165
Cdd:COG2319    124 RSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHSGAVTSVAFSPDGKLLASGSDdgtvrlWDlatgKLLRTLTGh 203

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1166 MKSVFDmkhsftedhyVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANnykrNCATFNPTDDLVL---NDGV 1242
Cdd:COG2319    204 TGAVRS----------VAFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGSV----RSVAFSPDGRLLAsgsADGT 269

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1243 --LWDVRSAQAIHKFDKFNMNISGV-FHPNG-------------LeWDLRTFHLLHTVPA 1286
Cdd:COG2319    270 vrLWDLATGELLRTLTGHSGGVNSVaFSPDGkllasgsddgtvrL-WDLATGKLLRTLTG 328
WD40 COG2319
WD40 repeat [General function prediction only];
1042-1285 7.93e-08

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 56.46  E-value: 7.93e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1042 QRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYN 1121
Cdd:COG2319     27 ALLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWD 106

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1122 VFSGQEEASYNCHNSAITHLEPSRDGSLLLTSAT------WS----QPLSALWGmksvfdmkHSFTEDHyVEFSKHSQDR 1191
Cdd:COG2319    107 LATGLLLRTLTGHTGAVRSVAFSPDGKTLASGSAdgtvrlWDlatgKLLRTLTG--------HSGAVTS-VAFSPDGKLL 177

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1192 VIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVRSAQAIHKFDKFNMNISGV- 1265
Cdd:COG2319    178 ASGSDDGTVRLWDLATGKLLRTL----TGHTGAVRSVAFSPDGKLLAsgsADGTvrLWDLATGKLLRTLTGHSGSVRSVa 253

                          250       260       270
                   ....*....|....*....|....*....|...
gi 2217347096 1266 FHPNG-------------LeWDLRTFHLLHTVP 1285
Cdd:COG2319    254 FSPDGrllasgsadgtvrL-WDLATGELLRTLT 285
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1300 5.99e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 53.11  E-value: 5.99e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200     93 TSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHTDWVNSVAFSPDGTFVASS------------------- 153

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGN--KLLTLFNPDLannykrNCATFNPTDDLVL---NDGV--LWD 1245
Cdd:cd00200    154 ---------------SQDGTI-------KLWDLRTGKcvATLTGHTGEV------NSVAFSPDGEKLLsssSDGTikLWD 205

                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2217347096 1246 VRSAQAIHKFDKFNMNISGV-FHPNGL------------EWDLRTFHLLHTVPAldqcrvvfnHTGTV 1300
Cdd:cd00200    206 LSTGKCLGTLRGHENGVNSVaFSPDGYllasgsedgtirVWDLRTGECVQTLSG---------HTNSV 264
WD40 COG2319
WD40 repeat [General function prediction only];
1092-1270 3.56e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 47.98  E-value: 3.56e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1092 DESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfd 1171
Cdd:COG2319    245 HSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHSGGVNSVAFSPDGKLLASG------------------ 306

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1172 mkhsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:COG2319    307 ----------------SDDGTV-------RLWDLATGKLLRTL----TGHTGAVRSVAFSPDGKTLAsgsDDGTvrLWDL 359

                          170       180
                   ....*....|....*....|....*
gi 2217347096 1247 RSAQAIHKFDKFNMNISGV-FHPNG 1270
Cdd:COG2319    360 ATGELLRTLTGHTGAVTSVaFSPDG 384
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1066-1154 1.70e-04

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 45.40  E-value: 1.70e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217347096 1066 VDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSR 1145
Cdd:cd00200    150 VASSSQDGTIKLWDLRTGKCVATLTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGHENGVNSVAFSP 229


                   ....*....
gi 2217347096 1146 DGSLLLTSA 1154
Cdd:cd00200    230 DGYLLASGS 238
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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