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Conserved domains on  [gi|2462562190|ref|XP_054175441|]
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chromatin assembly factor 1 subunit A isoform X2 [Homo sapiens]

Protein Classification

RLF2 family protein( domain architecture ID 12173874)

RLF2 (RAP1 localization factor 2) family protein similar to Saccharomyces cerevisiae chromatin assembly factor 1 subunit p90, which acts as a component of chromatin assembly factor 1 (CAF-1) that assembles histone octamers onto replicating DNA in vitro

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CAF1-p150_C2 pfam15539
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 646-935 0e+00

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


:

Pssm-ID: 434777  Cd Length: 288  Bit Score: 559.99  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 646 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLH 725
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGADLKVLQQFTACLLETVPSEEEQTPKASKREKRDQQILAQLLPLLH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 726 GNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTpsEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQV 805
Cdd:pfam15539  81 GNVNGSKVIIREFQEHCRRGLLSKGTGSPPSPSTSRPHTPT--EDAAIPSKARLKRIISENSVYEKRPDFRMCWYVHPQV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 806 LQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADT 885
Cdd:pfam15539 159 LQSFDQEHLPVPCQWSYVTMVPSAPREDSGSVPATGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQVGAEDMDGFQADT 238

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 2462562190 886 EEEEEEEGDCMIVDVPDAVEVQAPCGAASGAGGGVGVDTGKATLTASPLG 935
Cdd:pfam15539 239 EEEEEEDGDCMIMDIPDAGEVQAPCGTTSGAGGAVGMDTSKSPLPASPLG 288
CAF1-p150_N pfam15557
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-226 1.52e-150

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


:

Pssm-ID: 464767  Cd Length: 230  Bit Score: 443.56  E-value: 1.52e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190   1 MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDD----QGTSVQSKSPDLEASLDTLE 76
Cdd:pfam15557   1 MLEEPECGAPGARGAAAAMDCKDRPAFPVKKLIQARLPFKRLNLVPKEKAEDGSDDtrssQSAPVQSQVPDLETSLDTLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190  77 NNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGD 156
Cdd:pfam15557  81 NNCHMGSDIDFRPKLVNGKGPLDNFLRSRVKTSIGQSMVIIDLTEDSSEQPDSTVAHSKLNSAASPSREAVNGVREEAGD 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 157 QQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWS 226
Cdd:pfam15557 161 ERGLPKASQKDKLAFPEETLSDIPCKTEEEGVGSGGAERSGDSQKGSPQSCPKLTGGLRTCSEKDQDGWS 230
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 382-460 1.79e-27

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


:

Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 109.39  E-value: 1.79e-27
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2462562190 382 QRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVL 460
Cdd:pfam11600  86 LRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEEKRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPFEIKEHMVL 164
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 539-582 5.11e-19

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


:

Pssm-ID: 463507  Cd Length: 75  Bit Score: 82.16  E-value: 5.11e-19
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 2462562190 539 KLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSD 582
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
 
Name Accession Description Interval E-value
CAF1-p150_C2 pfam15539
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 646-935 0e+00

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 434777  Cd Length: 288  Bit Score: 559.99  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 646 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLH 725
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGADLKVLQQFTACLLETVPSEEEQTPKASKREKRDQQILAQLLPLLH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 726 GNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTpsEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQV 805
Cdd:pfam15539  81 GNVNGSKVIIREFQEHCRRGLLSKGTGSPPSPSTSRPHTPT--EDAAIPSKARLKRIISENSVYEKRPDFRMCWYVHPQV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 806 LQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADT 885
Cdd:pfam15539 159 LQSFDQEHLPVPCQWSYVTMVPSAPREDSGSVPATGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQVGAEDMDGFQADT 238

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 2462562190 886 EEEEEEEGDCMIVDVPDAVEVQAPCGAASGAGGGVGVDTGKATLTASPLG 935
Cdd:pfam15539 239 EEEEEEDGDCMIMDIPDAGEVQAPCGTTSGAGGAVGMDTSKSPLPASPLG 288
CAF1-p150_N pfam15557
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-226 1.52e-150

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 464767  Cd Length: 230  Bit Score: 443.56  E-value: 1.52e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190   1 MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDD----QGTSVQSKSPDLEASLDTLE 76
Cdd:pfam15557   1 MLEEPECGAPGARGAAAAMDCKDRPAFPVKKLIQARLPFKRLNLVPKEKAEDGSDDtrssQSAPVQSQVPDLETSLDTLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190  77 NNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGD 156
Cdd:pfam15557  81 NNCHMGSDIDFRPKLVNGKGPLDNFLRSRVKTSIGQSMVIIDLTEDSSEQPDSTVAHSKLNSAASPSREAVNGVREEAGD 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 157 QQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWS 226
Cdd:pfam15557 161 ERGLPKASQKDKLAFPEETLSDIPCKTEEEGVGSGGAERSGDSQKGSPQSCPKLTGGLRTCSEKDQDGWS 230
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 382-460 1.79e-27

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 109.39  E-value: 1.79e-27
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2462562190 382 QRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVL 460
Cdd:pfam11600  86 LRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEEKRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPFEIKEHMVL 164
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 539-582 5.11e-19

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


Pssm-ID: 463507  Cd Length: 75  Bit Score: 82.16  E-value: 5.11e-19
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 2462562190 539 KLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSD 582
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
 
Name Accession Description Interval E-value
CAF1-p150_C2 pfam15539
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 646-935 0e+00

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 434777  Cd Length: 288  Bit Score: 559.99  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 646 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLH 725
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGADLKVLQQFTACLLETVPSEEEQTPKASKREKRDQQILAQLLPLLH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 726 GNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTpsEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQV 805
Cdd:pfam15539  81 GNVNGSKVIIREFQEHCRRGLLSKGTGSPPSPSTSRPHTPT--EDAAIPSKARLKRIISENSVYEKRPDFRMCWYVHPQV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 806 LQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADT 885
Cdd:pfam15539 159 LQSFDQEHLPVPCQWSYVTMVPSAPREDSGSVPATGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQVGAEDMDGFQADT 238

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|
gi 2462562190 886 EEEEEEEGDCMIVDVPDAVEVQAPCGAASGAGGGVGVDTGKATLTASPLG 935
Cdd:pfam15539 239 EEEEEEDGDCMIMDIPDAGEVQAPCGTTSGAGGAVGMDTSKSPLPASPLG 288
CAF1-p150_N pfam15557
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-226 1.52e-150

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 464767  Cd Length: 230  Bit Score: 443.56  E-value: 1.52e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190   1 MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDD----QGTSVQSKSPDLEASLDTLE 76
Cdd:pfam15557   1 MLEEPECGAPGARGAAAAMDCKDRPAFPVKKLIQARLPFKRLNLVPKEKAEDGSDDtrssQSAPVQSQVPDLETSLDTLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190  77 NNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGD 156
Cdd:pfam15557  81 NNCHMGSDIDFRPKLVNGKGPLDNFLRSRVKTSIGQSMVIIDLTEDSSEQPDSTVAHSKLNSAASPSREAVNGVREEAGD 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562190 157 QQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWS 226
Cdd:pfam15557 161 ERGLPKASQKDKLAFPEETLSDIPCKTEEEGVGSGGAERSGDSQKGSPQSCPKLTGGLRTCSEKDQDGWS 230
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 382-460 1.79e-27

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 109.39  E-value: 1.79e-27
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2462562190 382 QRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVL 460
Cdd:pfam11600  86 LRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEEKRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPFEIKEHMVL 164
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 539-582 5.11e-19

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


Pssm-ID: 463507  Cd Length: 75  Bit Score: 82.16  E-value: 5.11e-19
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 2462562190 539 KLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSD 582
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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