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Conserved domains on  [gi|2462576457|ref|XP_054199555|]
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mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 5-125 3.08e-49

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


:

Pssm-ID: 214817  Cd Length: 124  Bit Score: 169.71  E-value: 3.08e-49
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462576457    5 ENVLQMLEAHMQ-SYKGNDPLGEWERYIQWVEENFPEN--KEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSD 81
Cdd:smart00777   1 EQQRQAFEAELQdLYEGDDPLDLWLRYIKWTEENYPQGgkESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEYCDE 80

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....
gi 2462576457   82 LHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRG 125
Cdd:smart00777  81 PRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
PKc_like super family cl21453
Protein Kinases, catalytic domain; The protein kinase superfamily is mainly composed of the ...
 786-821 3.40e-12

Protein Kinases, catalytic domain; The protein kinase superfamily is mainly composed of the catalytic domains of serine/threonine-specific and tyrosine-specific protein kinases. It also includes RIO kinases, which are atypical serine protein kinases, aminoglycoside phosphotransferases, and choline kinases. These proteins catalyze the transfer of the gamma-phosphoryl group from ATP to hydroxyl groups in specific substrates such as serine, threonine, or tyrosine residues of proteins.


The actual alignment was detected with superfamily member cd14028:

Pssm-ID: 473864 [Multi-domain]  Cd Length: 290  Bit Score: 67.95  E-value: 3.40e-12
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2462576457 786 LVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLK 821
Cdd:cd14028     1 SVYVDHLLGEGAFAQVYQATQLDLNDAKSNQKFVLK 36
 
Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 5-125 3.08e-49

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 214817  Cd Length: 124  Bit Score: 169.71  E-value: 3.08e-49
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462576457    5 ENVLQMLEAHMQ-SYKGNDPLGEWERYIQWVEENFPEN--KEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSD 81
Cdd:smart00777   1 EQQRQAFEAELQdLYEGDDPLDLWLRYIKWTEENYPQGgkESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEYCDE 80

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....
gi 2462576457   82 LHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRG 125
Cdd:smart00777  81 PRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
Mad3_BUB1_I pfam08311
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ...
 6-126 6.64e-46

Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 462420  Cd Length: 123  Bit Score: 160.00  E-value: 6.64e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462576457   6 NVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPEN--KEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLH 83
Cdd:pfam08311   1 QERQQFEEEIREYDGDDPLEPWLRYIKWTEESYPQGgkESGLLELLERCTRYFKDDERYKNDPRYLKLWLKYADFCDDPR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2462576457  84 QFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGI 126
Cdd:pfam08311  81 DIFQFLYSNGIGTKLALFYEEWAELLERQGRFKEADEIYQLGI 123
STKc_Bub1_vert cd14028
Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint ...
 786-821 3.40e-12

Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint protein Bub1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Bub1 (Budding uninhibited by benzimidazoles 1) contains an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding, a GLEBS motif for Bub3/kinetochore binding, and a C-terminal kinase domain. It is involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. Bub1 contributes to the inhibition of APC/C by phosphorylating its crucial cofactor, Cdc20, rendering it unable to activate APC/C. In addition, Bub1 facilitates the localization to kinetochores of other SAC and motor proteins including Mad1, Mad2, BubR1, and Plk1. It acts as the master organizer of the functional inner centromere. Bub1 also play roles in protecting sister chromatid cohesion and normal metaphase congression. The Bub1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.


Pssm-ID: 270930 [Multi-domain]  Cd Length: 290  Bit Score: 67.95  E-value: 3.40e-12
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2462576457 786 LVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLK 821
Cdd:cd14028     1 SVYVDHLLGEGAFAQVYQATQLDLNDAKSNQKFVLK 36
 
Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 5-125 3.08e-49

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 214817  Cd Length: 124  Bit Score: 169.71  E-value: 3.08e-49
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462576457    5 ENVLQMLEAHMQ-SYKGNDPLGEWERYIQWVEENFPEN--KEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSD 81
Cdd:smart00777   1 EQQRQAFEAELQdLYEGDDPLDLWLRYIKWTEENYPQGgkESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEYCDE 80

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....
gi 2462576457   82 LHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRG 125
Cdd:smart00777  81 PRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
Mad3_BUB1_I pfam08311
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ...
 6-126 6.64e-46

Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 462420  Cd Length: 123  Bit Score: 160.00  E-value: 6.64e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462576457   6 NVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPEN--KEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLH 83
Cdd:pfam08311   1 QERQQFEEEIREYDGDDPLEPWLRYIKWTEESYPQGgkESGLLELLERCTRYFKDDERYKNDPRYLKLWLKYADFCDDPR 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2462576457  84 QFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGI 126
Cdd:pfam08311  81 DIFQFLYSNGIGTKLALFYEEWAELLERQGRFKEADEIYQLGI 123
STKc_Bub1_vert cd14028
Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint ...
 786-821 3.40e-12

Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint protein Bub1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Bub1 (Budding uninhibited by benzimidazoles 1) contains an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding, a GLEBS motif for Bub3/kinetochore binding, and a C-terminal kinase domain. It is involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. Bub1 contributes to the inhibition of APC/C by phosphorylating its crucial cofactor, Cdc20, rendering it unable to activate APC/C. In addition, Bub1 facilitates the localization to kinetochores of other SAC and motor proteins including Mad1, Mad2, BubR1, and Plk1. It acts as the master organizer of the functional inner centromere. Bub1 also play roles in protecting sister chromatid cohesion and normal metaphase congression. The Bub1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.


Pssm-ID: 270930 [Multi-domain]  Cd Length: 290  Bit Score: 67.95  E-value: 3.40e-12
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2462576457 786 LVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLK 821
Cdd:cd14028     1 SVYVDHLLGEGAFAQVYQATQLDLNDAKSNQKFVLK 36
STKc_Bub1_BubR1 cd13981
Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 ...
 786-821 2.82e-03

Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 and BubR1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily is composed of Bub1 (Budding uninhibited by benzimidazoles 1), BubR1, and similar proteins. They contain an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding and a C-terminal kinase domain. Bub1 and BubR1 are involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. Impaired SAC leads to genomic instabilities and tumor development. Bub1 and BubR1 facilitate the localization of SAC proteins to kinetochores and regulate kinetochore-microtubule (K-MT) attachments. Repression studies of Bub1 and BubR1 show that they exert an additive effect in misalignment phenotypes and may function cooperatively or in parallel pathways in regulating K-MT attachments. The Bub1/BubR1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.


Pssm-ID: 270883 [Multi-domain]  Cd Length: 298  Bit Score: 40.80  E-value: 2.82e-03
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 2462576457 786 LVYVHHLLGEGAFAQVYEATQGDLNdaKNKQKFVLK 821
Cdd:cd13981     1 TYVISKELGEGGYASVYLAKDDDEQ--SDGSLVALK 34
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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