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Conserved domains on  [gi|2462537865|ref|XP_054230890|]
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cis-aconitate decarboxylase isoform X2 [Homo sapiens]

Protein Classification

MmgE/PrpD family protein( domain architecture ID 10005273)

MmgE/PrpD family protein similar to Mycobacterium tuberculosis 2-methylcitrate dehydratase that catalyzes the dehydration of 2-methylcitrate to form the cis isomer 2-methyl-aconitate

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PrpD COG2079
2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];
79-529 9.50e-118

2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];


:

Pssm-ID: 441682 [Multi-domain]  Cd Length: 446  Bit Score: 355.25  E-value: 9.50e-118
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865  79 TESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQPDiRLPPTYAAFVNG 158
Cdd:COG2079     1 TERLAEFAAGLRYEDLPAEVVERAKRRLLDTLGVALAGLREPPVRALRAAAAALGGPGGATVLGTGG-RLSPEDAALANG 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 159 VAIHSMDFDDTWHPAT-HPSGAVLPVLTALAEALPRSpkfsGLDLLLAFNVGIEVQGRLlhfAKEANDMPKR--FHPPSV 235
Cdd:COG2079    80 TAAHALDFDDTHLAAIgHPSADVIPAALAVAEALGAS----GRDLLTAIVAGYEVQGRL---GLAVGPSHYRrgWHHTGT 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 236 VGTLGSAAAASKFLGLSSTKCREALAIAVSHAGAPMA--NAATQTKPLHIGNAAKHGIEAAFLAMLGLQGNKQVLDLEAG 313
Cdd:COG2079   153 LGTFGAAAAAARLLGLDAEQTAHALGIAASQAAGLRQylRFGTMTKPLHAGFAARNGVLAALLARAGFTGPPDILEGPFG 232
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 314 FGAFYANYSPKVLPSIASY--SWLLDqqDVAFKRFPAHLSTHWVADAAAsvrkHLVAERALLPTDyIKRIVLRIP--NVQ 389
Cdd:COG2079   233 FFAAFAGGEFDPEALTDGLgeRWEIL--ETSFKPYPACRHTHAAIDAAL----ALRAEHGLDPDD-IERIEVETHpaALR 305
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 390 YVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRPQVRELLSKVELEypPDnlPSFNILY-----CEISVTL 464
Cdd:COG2079   306 IIGDPDPATPEDAKFSLPYCVAVALLDGRLGLDDFEDERLADPDVRALAAKVTVV--ED--PELDARYpaarpARVTVTL 381
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2462537865 465 KDGATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTL 529
Cdd:COG2079   382 KDGRTLEAEVDYPKGHPENPLSDEELEAKFRALAAPVLGAARAERLLDAVRRLEDLPDVAELLAL 446
 
Name Accession Description Interval E-value
PrpD COG2079
2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];
79-529 9.50e-118

2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];


Pssm-ID: 441682 [Multi-domain]  Cd Length: 446  Bit Score: 355.25  E-value: 9.50e-118
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865  79 TESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQPDiRLPPTYAAFVNG 158
Cdd:COG2079     1 TERLAEFAAGLRYEDLPAEVVERAKRRLLDTLGVALAGLREPPVRALRAAAAALGGPGGATVLGTGG-RLSPEDAALANG 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 159 VAIHSMDFDDTWHPAT-HPSGAVLPVLTALAEALPRSpkfsGLDLLLAFNVGIEVQGRLlhfAKEANDMPKR--FHPPSV 235
Cdd:COG2079    80 TAAHALDFDDTHLAAIgHPSADVIPAALAVAEALGAS----GRDLLTAIVAGYEVQGRL---GLAVGPSHYRrgWHHTGT 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 236 VGTLGSAAAASKFLGLSSTKCREALAIAVSHAGAPMA--NAATQTKPLHIGNAAKHGIEAAFLAMLGLQGNKQVLDLEAG 313
Cdd:COG2079   153 LGTFGAAAAAARLLGLDAEQTAHALGIAASQAAGLRQylRFGTMTKPLHAGFAARNGVLAALLARAGFTGPPDILEGPFG 232
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 314 FGAFYANYSPKVLPSIASY--SWLLDqqDVAFKRFPAHLSTHWVADAAAsvrkHLVAERALLPTDyIKRIVLRIP--NVQ 389
Cdd:COG2079   233 FFAAFAGGEFDPEALTDGLgeRWEIL--ETSFKPYPACRHTHAAIDAAL----ALRAEHGLDPDD-IERIEVETHpaALR 305
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 390 YVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRPQVRELLSKVELEypPDnlPSFNILY-----CEISVTL 464
Cdd:COG2079   306 IIGDPDPATPEDAKFSLPYCVAVALLDGRLGLDDFEDERLADPDVRALAAKVTVV--ED--PELDARYpaarpARVTVTL 381
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2462537865 465 KDGATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTL 529
Cdd:COG2079   382 KDGRTLEAEVDYPKGHPENPLSDEELEAKFRALAAPVLGAARAERLLDAVRRLEDLPDVAELLAL 446
MmgE_PrpD pfam03972
MmgE/PrpD N-terminal domain; This entry represents the N-terminal domain of 2-methylcitrate ...
80-323 3.33e-81

MmgE/PrpD N-terminal domain; This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyzes the third step of the 2-methylcitric acid cycle.


Pssm-ID: 461115  Cd Length: 244  Bit Score: 253.94  E-value: 3.33e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865  80 ESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQpDIRLPPTYAAFVNGV 159
Cdd:pfam03972   1 EQLADYVAGLRYDDLPDEVVERAKRCLLDTLGCALAGLRHPPVTKALGAVPGTGGPGGATVIGT-GYRLDPVNAAFANGA 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 160 AIHSMDFDDTWHPA-THPSGAVLPVLTALAEALPRSpkfsGLDLLLAFNVGIEVQGRLlhfAKEANDMPKRFHPPSVVGT 238
Cdd:pfam03972  80 AIHALDFDDTHLAAeWHHPSDNLPAILAVAEHLGRS----GRDLLTALVVGYEIQGRL---ALANSFNHYGRHDHVTLGT 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 239 LGSAAAASKFLGLSSTKCREALAIAVSHAGAPMANAATQTKPL---HIGNAAKHGIEAAFLAMLGLQGNKQVLDLEAGFG 315
Cdd:pfam03972 153 FGAAAAAAKLLGLDAEQIANALGIAATQAAGLRQYRHAPNTSKrkwAAGDAARNGVFAALLAKRGFTGPSDLLEGEWGFY 232

                  ....*...
gi 2462537865 316 AFYANYSP 323
Cdd:pfam03972 233 DVLSGGKD 240
prpD PRK09425
bifunctional 2-methylcitrate dehydratase/aconitate hydratase;
147-525 5.52e-09

bifunctional 2-methylcitrate dehydratase/aconitate hydratase;


Pssm-ID: 181845  Cd Length: 480  Bit Score: 58.35  E-value: 5.52e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 147 RLPPTYAAFVNGVAIHSMDFDDTWHPAT--HPS---GAVLpvltALAEALPRS------PKFSGLDLLLAFNVGIEVQGR 215
Cdd:PRK09425   82 QLDPVQAAFNIGAMIRWLDFNDTWLAAEwgHPSdnlGGIL----AVADYLSRKavaegkEPLTMRDVLTAMIKAHEIQGV 157
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 216 LlhfAKEaNDMPKrfhppsvVG-------TLGSAAAASKFLGLSstkcREALAIAVSHA---GAPM------ANAATQtK 279
Cdd:PRK09425  158 L---ALE-NSFNR-------VGldhvllvKVASTAVVAKLLGGT----REEILNALSLAwvdGQSLrtyrhaPNTGSR-K 221
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 280 PLHIGNAAKHGIEAAFLAMLGLQGNKQVLdlEAGFGAFYA---NYSPKVLP-SIASYSwlldQQDVAFK-RFPA--HLST 352
Cdd:PRK09425  222 SWAAGDATSRAVRLALIALTGEMGYPSAL--TAPTWGFYDvlfKGKPFRFQrPYGSYV----MENVLFKiSFPAefHAQT 295
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 353 hwvadAA-ASVRKH-LVAERAllptDYIKRIVLRI--PNVQYVNRPFPVSEHEAR-HSFQY-VACAmLLDGGITVPSFHE 426
Cdd:PRK09425  296 -----AVeAAVTLHpQVKARL----DDIERITIRTheSAIRIIDKKGPLNNPADRdHCLQYmVAVP-LLFGRLTAEDYED 365
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 427 CQINRPQVRELLSKVELEYPPDnlpsFNILYCE---------ISVTLKDGATfTDRSDTFY--GHWRK-----PLsqedL 490
Cdd:PRK09425  366 AVAADPRIDALREKMVVVEDPQ----FTRDYLDpekrsianaVQVFFKDGSS-TEEVEVEYpiGHRRRraegiPL----L 436
                         410       420       430
                  ....*....|....*....|....*....|....*
gi 2462537865 491 EEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSV 525
Cdd:PRK09425  437 VEKFKANLATRFPAKQQQRILDLCLDQERLEAMPV 471
 
Name Accession Description Interval E-value
PrpD COG2079
2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];
79-529 9.50e-118

2-methylcitrate dehydratase PrpD [Carbohydrate transport and metabolism];


Pssm-ID: 441682 [Multi-domain]  Cd Length: 446  Bit Score: 355.25  E-value: 9.50e-118
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865  79 TESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQPDiRLPPTYAAFVNG 158
Cdd:COG2079     1 TERLAEFAAGLRYEDLPAEVVERAKRRLLDTLGVALAGLREPPVRALRAAAAALGGPGGATVLGTGG-RLSPEDAALANG 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 159 VAIHSMDFDDTWHPAT-HPSGAVLPVLTALAEALPRSpkfsGLDLLLAFNVGIEVQGRLlhfAKEANDMPKR--FHPPSV 235
Cdd:COG2079    80 TAAHALDFDDTHLAAIgHPSADVIPAALAVAEALGAS----GRDLLTAIVAGYEVQGRL---GLAVGPSHYRrgWHHTGT 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 236 VGTLGSAAAASKFLGLSSTKCREALAIAVSHAGAPMA--NAATQTKPLHIGNAAKHGIEAAFLAMLGLQGNKQVLDLEAG 313
Cdd:COG2079   153 LGTFGAAAAAARLLGLDAEQTAHALGIAASQAAGLRQylRFGTMTKPLHAGFAARNGVLAALLARAGFTGPPDILEGPFG 232
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 314 FGAFYANYSPKVLPSIASY--SWLLDqqDVAFKRFPAHLSTHWVADAAAsvrkHLVAERALLPTDyIKRIVLRIP--NVQ 389
Cdd:COG2079   233 FFAAFAGGEFDPEALTDGLgeRWEIL--ETSFKPYPACRHTHAAIDAAL----ALRAEHGLDPDD-IERIEVETHpaALR 305
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 390 YVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRPQVRELLSKVELEypPDnlPSFNILY-----CEISVTL 464
Cdd:COG2079   306 IIGDPDPATPEDAKFSLPYCVAVALLDGRLGLDDFEDERLADPDVRALAAKVTVV--ED--PELDARYpaarpARVTVTL 381
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2462537865 465 KDGATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTL 529
Cdd:COG2079   382 KDGRTLEAEVDYPKGHPENPLSDEELEAKFRALAAPVLGAARAERLLDAVRRLEDLPDVAELLAL 446
MmgE_PrpD pfam03972
MmgE/PrpD N-terminal domain; This entry represents the N-terminal domain of 2-methylcitrate ...
80-323 3.33e-81

MmgE/PrpD N-terminal domain; This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyzes the third step of the 2-methylcitric acid cycle.


Pssm-ID: 461115  Cd Length: 244  Bit Score: 253.94  E-value: 3.33e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865  80 ESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQpDIRLPPTYAAFVNGV 159
Cdd:pfam03972   1 EQLADYVAGLRYDDLPDEVVERAKRCLLDTLGCALAGLRHPPVTKALGAVPGTGGPGGATVIGT-GYRLDPVNAAFANGA 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 160 AIHSMDFDDTWHPA-THPSGAVLPVLTALAEALPRSpkfsGLDLLLAFNVGIEVQGRLlhfAKEANDMPKRFHPPSVVGT 238
Cdd:pfam03972  80 AIHALDFDDTHLAAeWHHPSDNLPAILAVAEHLGRS----GRDLLTALVVGYEIQGRL---ALANSFNHYGRHDHVTLGT 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 239 LGSAAAASKFLGLSSTKCREALAIAVSHAGAPMANAATQTKPL---HIGNAAKHGIEAAFLAMLGLQGNKQVLDLEAGFG 315
Cdd:pfam03972 153 FGAAAAAAKLLGLDAEQIANALGIAATQAAGLRQYRHAPNTSKrkwAAGDAARNGVFAALLAKRGFTGPSDLLEGEWGFY 232

                  ....*...
gi 2462537865 316 AFYANYSP 323
Cdd:pfam03972 233 DVLSGGKD 240
MmgE_PrpD_C pfam19305
MmgE/PrpD C-terminal domain; This entry represents the C-terminal domain of 2-methylcitrate ...
346-519 5.59e-44

MmgE/PrpD C-terminal domain; This entry represents the C-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyzes the third step of the 2-methylcitric acid cycle. This domain is related to the serine dehydratase beta chain.


Pssm-ID: 466033 [Multi-domain]  Cd Length: 173  Bit Score: 153.85  E-value: 5.59e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 346 FPAHLSTHWVADAAASVRKhlvaERALLPTDyIKRIVLRIP--NVQYVNRP-FPVSEHEARHSFQYVACAMLLDGGITVP 422
Cdd:pfam19305   1 YPAERHTHTAIDAALQLHR----EHGLRPDD-IESIVIRTHeaALRIIGKKgPPATRADADHSLQYVVAVALLDGRLTLE 75
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 423 SFHECQINRPQVRELLSKVELEYPPDNLPSFNILYCEISVTLKD-GATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKM 501
Cdd:pfam19305  76 DFEDERAADPRIDALRDKVEVVEDPEFTADYPEKRSIVVIVLLDgGRTLEVRVEYPKGHPRRPLSREELEEKFRRLAAGV 155
                         170
                  ....*....|....*...
gi 2462537865 502 LSWDTVESLIKIVKNLED 519
Cdd:pfam19305 156 LPESEAERIIIIVLVLEL 173
prpD PRK09425
bifunctional 2-methylcitrate dehydratase/aconitate hydratase;
147-525 5.52e-09

bifunctional 2-methylcitrate dehydratase/aconitate hydratase;


Pssm-ID: 181845  Cd Length: 480  Bit Score: 58.35  E-value: 5.52e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 147 RLPPTYAAFVNGVAIHSMDFDDTWHPAT--HPS---GAVLpvltALAEALPRS------PKFSGLDLLLAFNVGIEVQGR 215
Cdd:PRK09425   82 QLDPVQAAFNIGAMIRWLDFNDTWLAAEwgHPSdnlGGIL----AVADYLSRKavaegkEPLTMRDVLTAMIKAHEIQGV 157
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 216 LlhfAKEaNDMPKrfhppsvVG-------TLGSAAAASKFLGLSstkcREALAIAVSHA---GAPM------ANAATQtK 279
Cdd:PRK09425  158 L---ALE-NSFNR-------VGldhvllvKVASTAVVAKLLGGT----REEILNALSLAwvdGQSLrtyrhaPNTGSR-K 221
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 280 PLHIGNAAKHGIEAAFLAMLGLQGNKQVLdlEAGFGAFYA---NYSPKVLP-SIASYSwlldQQDVAFK-RFPA--HLST 352
Cdd:PRK09425  222 SWAAGDATSRAVRLALIALTGEMGYPSAL--TAPTWGFYDvlfKGKPFRFQrPYGSYV----MENVLFKiSFPAefHAQT 295
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 353 hwvadAA-ASVRKH-LVAERAllptDYIKRIVLRI--PNVQYVNRPFPVSEHEAR-HSFQY-VACAmLLDGGITVPSFHE 426
Cdd:PRK09425  296 -----AVeAAVTLHpQVKARL----DDIERITIRTheSAIRIIDKKGPLNNPADRdHCLQYmVAVP-LLFGRLTAEDYED 365
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462537865 427 CQINRPQVRELLSKVELEYPPDnlpsFNILYCE---------ISVTLKDGATfTDRSDTFY--GHWRK-----PLsqedL 490
Cdd:PRK09425  366 AVAADPRIDALREKMVVVEDPQ----FTRDYLDpekrsianaVQVFFKDGSS-TEEVEVEYpiGHRRRraegiPL----L 436
                         410       420       430
                  ....*....|....*....|....*....|....*
gi 2462537865 491 EEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSV 525
Cdd:PRK09425  437 VEKFKANLATRFPAKQQQRILDLCLDQERLEAMPV 471
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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