P-loop containing Nucleoside Triphosphate Hydrolases; Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a hydrophobic residue). The Walker A and B motifs bind the beta-gamma phosphate moiety of the bound nucleotide (typically ATP or GTP) and the Mg2+ cation, respectively. The P-loop NTPases are involved in diverse cellular functions, and they can be divided into two major structural classes: the KG (kinase-GTPase) class which includes Ras-like GTPases and its circularly permutated YlqF-like; and the ASCE (additional strand catalytic E) class which includes ATPase Binding Cassette (ABC), DExD/H-like helicases, 4Fe-4S iron sulfur cluster binding proteins of NifH family, RecA-like F1-ATPases, and ATPases Associated with a wide variety of Activities (AAA). Also included are a diverse set of nucleotide/nucleoside kinase families.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647 245 NFGFCGRSGSGKSSLINSLRGLNNGDPQSA--GRSHCDrMEPFRFIEGEFQQIVLWEIPYPRTFSSSSVVFDANMGFEKl 322
Cdd:cd04104   3 NIAVTGESGAGKSSFINALRGIGHEEEGAAptGVVETT-MKRTPYPHPKFPNVTLWDLPGIGSTAFPPDDYLEEMKFSE- 80

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*
gi 71985647 323 YEshklklfkrLFILIPDGAPTDEDITFARVALSRRTSITFLLTKSDEDLDAENR 377
Cdd:cd04104  81 YD---------FFIIISSTRFSSNDVKLAKAIQMMGKKFYFVRTKVDSDLSNEQR 126

p47 GTPase family includes IGTP, TGTP/Mg21, IRG-47, GTPI, LRG-47, and IIGP1; The p47 GTPase family consists of several highly homologous proteins, including IGTP, TGTP/Mg21, IRG-47, GTPI, LRG-47, and IIGP1. They are found in higher eukaryotes where they play a role in immune resistance against intracellular pathogens. p47 proteins exist at low resting levels in mouse cells, but are strongly induced by Type II interferon (IFN-gamma). ITGP is critical for resistance to Toxoplasma gondii infection and in involved in inhibition of Coxsackievirus-B3-induced apoptosis. TGTP was shown to limit vesicular stomatitis virus (VSV) infection of fibroblasts in vitro. IRG-47 is involved in resistance to T. gondii infection. LRG-47 has been implicated in resistance to T. gondii, Listeria monocytogenes, Leishmania, and mycobacterial infections. IIGP1 has been shown to localize to the ER and to the Golgi membranes in IFN-induced cells and inflamed tissues. In macrophages, IIGP1 interacts with hook3, a microtubule binding protein that participates in the organization of the cis-Golgi compartment.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647 245 NFGFCGRSGSGKSSLINSLRGLNNGDPQSA--GRSHCDrMEPFRFIEGEFQQIVLWEIPYPRTFSSSSVVFDANMGFEKl 322
Cdd:cd04104   3 NIAVTGESGAGKSSFINALRGIGHEEEGAAptGVVETT-MKRTPYPHPKFPNVTLWDLPGIGSTAFPPDDYLEEMKFSE- 80

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*
gi 71985647 323 YEshklklfkrLFILIPDGAPTDEDITFARVALSRRTSITFLLTKSDEDLDAENR 377
Cdd:cd04104  81 YD---------FFIIISSTRFSSNDVKLAKAIQMMGKKFYFVRTKVDSDLSNEQR 126

Interferon-inducible GTPase (IIGP); Interferon-inducible GTPase (IIGP) is thought to play a role in in intracellular defence. IIGP is predominantly associated with the Golgi apparatus and also localizes to the endoplasmic reticulum and exerts a distinct role in IFN-induced intracellular membrane trafficking or processing.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647   239 IDANRKNFGFCGRSGSGKSSLINSLRGLNNGDPQSAGRSHCD-RMEPFRFIEGEFQQIVLWEIPYPRTFSSSSVVFDANM 317
Cdd:pfam05049  31 ISSAPLKIAVTGDSGNGKSSFINALRGIGHEEDGSAPTGVVEtTMKRTPYSHPHFPNVVLWDLPGLGATNFTVESYLEEM 110

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 71985647   318 GFEKlYEshklklfkrLFILIPDGAPTDEDITFARVALSRRTSITFLLTKSDEDLDAE 375
Cdd:pfam05049 111 KFSE-YD---------FFIIISSERFSLNDVKLAKAIQRMGKRFYFVRTKLDSDLSNE 158

p47 GTPase family includes IGTP, TGTP/Mg21, IRG-47, GTPI, LRG-47, and IIGP1; The p47 GTPase family consists of several highly homologous proteins, including IGTP, TGTP/Mg21, IRG-47, GTPI, LRG-47, and IIGP1. They are found in higher eukaryotes where they play a role in immune resistance against intracellular pathogens. p47 proteins exist at low resting levels in mouse cells, but are strongly induced by Type II interferon (IFN-gamma). ITGP is critical for resistance to Toxoplasma gondii infection and in involved in inhibition of Coxsackievirus-B3-induced apoptosis. TGTP was shown to limit vesicular stomatitis virus (VSV) infection of fibroblasts in vitro. IRG-47 is involved in resistance to T. gondii infection. LRG-47 has been implicated in resistance to T. gondii, Listeria monocytogenes, Leishmania, and mycobacterial infections. IIGP1 has been shown to localize to the ER and to the Golgi membranes in IFN-induced cells and inflamed tissues. In macrophages, IIGP1 interacts with hook3, a microtubule binding protein that participates in the organization of the cis-Golgi compartment.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647 245 NFGFCGRSGSGKSSLINSLRGLNNGDPQSA--GRSHCDrMEPFRFIEGEFQQIVLWEIPYPRTFSSSSVVFDANMGFEKl 322
Cdd:cd04104   3 NIAVTGESGAGKSSFINALRGIGHEEEGAAptGVVETT-MKRTPYPHPKFPNVTLWDLPGIGSTAFPPDDYLEEMKFSE- 80

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*
gi 71985647 323 YEshklklfkrLFILIPDGAPTDEDITFARVALSRRTSITFLLTKSDEDLDAENR 377
Cdd:cd04104  81 YD---------FFIIISSTRFSSNDVKLAKAIQMMGKKFYFVRTKVDSDLSNEQR 126

Interferon-inducible GTPase (IIGP); Interferon-inducible GTPase (IIGP) is thought to play a role in in intracellular defence. IIGP is predominantly associated with the Golgi apparatus and also localizes to the endoplasmic reticulum and exerts a distinct role in IFN-induced intracellular membrane trafficking or processing.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647   239 IDANRKNFGFCGRSGSGKSSLINSLRGLNNGDPQSAGRSHCD-RMEPFRFIEGEFQQIVLWEIPYPRTFSSSSVVFDANM 317
Cdd:pfam05049  31 ISSAPLKIAVTGDSGNGKSSFINALRGIGHEEDGSAPTGVVEtTMKRTPYSHPHFPNVVLWDLPGLGATNFTVESYLEEM 110

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 71985647   318 GFEKlYEshklklfkrLFILIPDGAPTDEDITFARVALSRRTSITFLLTKSDEDLDAE 375
Cdd:pfam05049 111 KFSE-YD---------FFIIISSERFSLNDVKLAKAIQRMGKRFYFVRTKLDSDLSNE 158

Rat sarcoma (Ras)-like superfamily of small guanosine triphosphatases (GTPases); Ras-like GTPase superfamily. The Ras-like superfamily of small GTPases consists of several families with an extremely high degree of structural and functional similarity. The Ras superfamily is divided into at least four families in eukaryotes: the Ras, Rho, Rab, and Sar1/Arf families. This superfamily also includes proteins like the GTP translation factors, Era-like GTPases, and G-alpha chain of the heterotrimeric G proteins. Members of the Ras superfamily regulate a wide variety of cellular functions: the Ras family regulates gene expression, the Rho family regulates cytoskeletal reorganization and gene expression, the Rab and Sar1/Arf families regulate vesicle trafficking, and the Ran family regulates nucleocytoplasmic transport and microtubule organization. The GTP translation factor family regulates initiation, elongation, termination, and release in translation, and the Era-like GTPase family regulates cell division, sporulation, and DNA replication. Members of the Ras superfamily are identified by the GTP binding site, which is made up of five characteristic sequence motifs, and the switch I and switch II regions.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71985647 538 GFIGGRGVGKSSLIDAMRGmssKNPLSATKLNNRSKAgsCERFEFDDNVLKYSVTLYELSYPKKISSYFEFIDLVNVASF 617
Cdd:cd00882   1 VVVGRGGVGKSSLLNALLG---GEVGEVSDVPGTTRD--PDVYVKELDKGKVKLVLVDTPGLDEFGGLGREELARLLLRG 75

                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 71985647 618 TALFILV---DQTPSEQDLAF--AKIAYRRNTTILFLISKCDK 655
Cdd:cd00882  76 ADLILLVvdsTDRESEEDAKLliLRRLRKEGIPIILVGNKIDL 118

ATP-binding cassette domain 2 of multidrug resistance-associated protein; The ABC subfamily C is also known as MRP (multidrug resistance-associated protein). Some of the MRP members have five additional transmembrane segments in their N-terminus, but the function of these additional membrane-spanning domains is not clear. The MRP was found in the multidrug-resistance lung cancer cell in which p-glycoprotein was not overexpressed. MRP exports glutathione by drug stimulation, as well as, certain substrates in conjugated forms with anions, such as glutathione, glucuronate, and sulfate.

                        10
                ....*....|....*..
gi 71985647 247 GFCGRSGSGKSSLINSL 263
Cdd:cd03244  34 GIVGRTGSGKSSLLLAL 50